CN106011034A - Bacillus subtilis strain and application thereof - Google Patents
Bacillus subtilis strain and application thereof Download PDFInfo
- Publication number
- CN106011034A CN106011034A CN201610625978.6A CN201610625978A CN106011034A CN 106011034 A CN106011034 A CN 106011034A CN 201610625978 A CN201610625978 A CN 201610625978A CN 106011034 A CN106011034 A CN 106011034A
- Authority
- CN
- China
- Prior art keywords
- bacillus subtilis
- water
- waste water
- strain
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Belonging to the field of environmental microorganism application, the invention discloses a Bacillus subtilis strain and application thereof. The strain is named as Bacillus subtilis CM-A12, is currently preserved in China Center For Type Culture Collection (called CCTCC for short) with a preservation number of CCTCC NO:M 2015688, and the preservation date is November 23, 2015. The Bacillus subtilis strain provided by the invention has the advantages of simple cultivation condition, high temperature resistance, acid and alkali resistance, and high salinity resistance, can secrete protease, amylase and cellulase, and can effectively improve water quality and reduce COD and ammonia nitrogen in water.
Description
Technical field
The present invention relates to environmental microorganism application, particularly relate to a bacillus subtilis strain and application thereof.
Background technology
Along with socioeconomic development, the increase of population, improving constantly of people's living standard, the demand of water resource and consumption not
Breaking and increase, the discharge capacity of corresponding municipal sewage and industrial wastewater is also being on the increase.But sewage treatment facility is delayed and dirty
Dye controls the most capable so that global water resources is faced with two large problems: water environment pollution and shortage of water resources.This is in some states
Family and area, the especially developing country including China, aggravation also has the trend of aggravation.For ensureing human society
Economic sustainable development, water environment protection and improvement have become the significant problem of society.
In water environment protection and improvement, microorganism plays very important effect, serves as the role of " natural surroundings bodyguard ".
Its metabolic type has multiformity, the Degradation and Transformation of pollutant, resource recycle and the aspect such as ecological environmental protection all
There is great potential.
In microbial bacterial agent in terms of being applied to environmental conservation, the kind of microorganism mainly has yeast, lactic acid bacteria, spore bar
Bacterium and photosynthetic bacteria etc..Wherein bacillus cereus can be formed chemical factors tools such as heat, dry, good fortune is penetrated, chemicalses intracellular
There is the spore of the strongest resistance, there is the function resisting unsuitable environmental condition.Except vitality is strong, bacillus cereus can produce multiple
The outer hydrolase of the born of the same parents such as highly active protease, amylase, lipase, pectase, glucanase, cellulase, decomposition has
The ability of machine matter is strong, some difficult macromolecular substances decomposed in waste water can be changed into the small-molecule substance that can be utilized by self, point
Solve the Organic substance producing foul gas, there is the function of deodorization, can also effectively decompose simultaneously the feedstuff of deposition in aquaculture system,
Excreta, animal residue etc., promote material circulation and the purification of water quality of breeding environment.
Such as: application publication number is that the Chinese invention patent application document of CN103011424A discloses a kind of bacillus subtilis
DS3 application in degraded saponin waste water organic pollution.This bacillus subtilis DS3 is preserved in China typical culture collection
Center, preserving number is CCTCC NO:M 2010146.This bacillus subtilis DS3 is in high concentration sulphate ionic environment
Having the ability of organic pollution in stronger energy for growth and good removal saponin waste water, this bacterial strain can go within 72 hours
Except the COD 2150mg/L in saponin waste water, removal efficiency reaches 50.71%.
But above-mentioned bacterial strains is only in saponin waste water organic pollution, its application surface relative narrower.Should therefore, it is necessary to filter out
Wider with face, water treatment effect more preferably bacterial strain, to improve the promotional value of bacterial strain.
Summary of the invention
The invention provides a kind of bacillus subtilis strain and application thereof, this Bacillus strain condition of culture is simple, high temperature resistant,
Acid and alkali-resistance, resistance to high salt, it is possible to extracellular proteinase, amylase, cellulase, be effectively improved water quality, reduce the COD in water body,
Ammonia nitrogen, is a kind of multi-functional bacterial strain.
A kind of bacillus subtilis strain, it is characterised in that named bacillus subtilis (Bacillus subtilis) CM-A12,
Deposit number is CCTCC NO:M 2015688, and preservation date is on November 23rd, 2015.
The Chinese Typical Representative that described bacillus subtilis strain is deposited in Wuhan University of Wuhan, China city on November 23rd, 2015 is cultivated
CCTCC (is called for short) in thing preservation center, and deposit number is CCTCC NO:M 2015688, named bacillus subtilis
(Bacillus subtilis)CM-A12。
The biological property of described bacillus subtilis (Bacillus subtilis) CM-A12 is: bacterium colony is ecru, dashes forward in surface
Play fold, be dried tarnish；Under microscope, strain cell is brevibacterium.
The 16S rDNA sequence of described bacillus subtilis (Bacillus subtilis) CM-A12 is as shown in SEQ ID NO.1.
The invention provides the cultural method of a kind of bacillus subtilis strain, described bacillus subtilis strain is lived by the method
After change, be inoculated into original ph be 4～9, temperature be 25～40 DEG C, the mass concentration of salt be 0～14% culture medium in cultivate.
The invention provides the application in water remediation or waste water process of a kind of described bacillus subtilis strain.
Specifically, described water body is river course or lake；Described waste water is sanitary sewage, breeding wastewater, dyeing waste water, food row
Industry waste water or paper waste.
The application in processing waste water of the described bacillus subtilis strain, including: described bacillus subtilis bacterium is directly added
Biochemical treatment is carried out to water body or waste water；Or after immobilization, then water body or waste water are carried out biochemical treatment.
Described bacillus subtilis strain directly can add to water body or waste water as liquid bacterium solution or dry bacteria；Described solid
Determining includes: be immobilized onto in microsphere by bacterial strain, or carries out bio-film colonization and be fixed on filler by bacterial strain；Waste water is through directly throwing
When the microbial inoculum added or immobilized microsphere or filler, the pollutant in waste water are degraded by bacterial strain.
The invention provides a kind of microbial inoculum comprising described bacillus subtilis strain.Described microbial inoculum can be liquid bacterium solution or
The mycopowder of person's solid.
The invention provides a kind of microbe microsphere comprising described bacillus subtilis strain.
The Bacillus strain condition of culture that the present invention provides is simple, high temperature resistant, acid and alkali-resistance, resistance to high salt, it is possible to extracellular proteinase,
Amylase, cellulase, and it is effectively improved water quality, reduce the COD in water body, ammonia nitrogen.
Accompanying drawing explanation
Fig. 1 is cytological map under bacillus subtilis of the present invention (Bacillus subtilis) CM-A12 colonial morphology picture and microscope
Sheet.
Fig. 2 is that bacillus subtilis of the present invention (Bacillus subtilis) CM-A12 builds based on 16S rDNA sequence homology
Phylogenetic tree.
Fig. 3 is the impact that bacillus subtilis of the present invention (Bacillus subtilis) CM-A12 is grown by pH value.
Fig. 4 is the impact that bacillus subtilis of the present invention (Bacillus subtilis) CM-A12 is grown by temperature.
Fig. 5 is the impact that bacillus subtilis of the present invention (Bacillus subtilis) CM-A12 is grown by salinity.
Fig. 6 is that bacillus subtilis in embodiment 4 (Bacillus subtilis) CM-A12 is to ammonia nitrogen, the removal effect of COD.
Detailed description of the invention
The separation screening of embodiment 1 sporeformer
1, the collection of mud
Gather the mud of culture pond, water sample 7 parts, weigh 10g mud (water sample pipettes 10mL) respectively and be equipped with 90mL
In the conical flask of sterile saline, in 150r/min shaking table, shake 0.5h, take out and put into water-bath 30min in 80 DEG C of water-baths,
Use decimal dilution method that sample liquid dilutes certain multiple, standby.
Take 0.1mL dilute sample liquid with on solid LB media be coated with, in 30 DEG C cultivate 48h, select grow fine, bacterium colony
Plesiomorphism and the most bacterium colony of number, isolated and purified until the consistent single bacterium of colonial morphology, it is thus achieved that 12 strain sporeformer.
2, the screening of enzyme bacillus cereus is produced
(1) cellulase detection:
Sodium carboxymethyl cellulose plating medium (g/L): CMC-Na 15.0, NaCl 5.0, KH2PO41.0, MgSO40.2,
Peptone 10.0, yeast powder 5.0, agar 18.0, pH 7.2-7.5.
By in the bacterial strain dibbling that is separated to cellulose culture medium, cultivate 48-72h for 37 DEG C, dye with 1g/L Congo red dye liquor
20min, discards dye liquor, adds 1mol/L NaCl solution, after washing 20min, if there is hydrolysis in periphery of bacterial colonies,
Illustrate that CMC has been hydrolyzed, measure the ratio of transparent circle diameter and colony diameter.
(2) protease detection:
Milk culturemedium: skim milk 50ml, distilled water 50ml, agar powder 1.5g.
By in the bacterial strain dibbling that is separated to milk culturemedium, cultivate 24-48h for 37 DEG C, say if periphery of bacterial colonies has transparent circle to produce
Bright casein has been hydrolyzed, and measures the ratio of transparent circle diameter and colony diameter.
(3) amylase detection:
Starch culture-medium (g/L): peptone 10.0g, Carnis Bovis seu Bubali cream 5.0g, NaCl 5.0g, soluble starch 2.0g, pH 7.2-7.5,
Agar powder 20g.
By in the bacterial strain dibbling that is separated to culture medium, cultivate 24-48h for 37 DEG C, drip appropriate iodine liquid in media surface and make uniformly
It is covered with whole flat board, if water white transparency circle occurs in periphery of bacterial colonies, illustrates that starch is hydrolyzed, measure transparent circle diameter and bacterium colony
The ratio of diameter.
Through producing enzyme experiment, screening obtains the bacterial strain that a strain energy amylase, protease, cellulase and yield of enzyme are higher
CM-A12.The enzymatic productivity of this bacterial strain CM-A12 is as shown in table 1 below.
Table 1
| Enzyme | Cellulase | Protease | Amylase |
| Enzymatic productivity (hydrolytic circle (mm)/colony diameter (mm)) | 45/11 | 34/8 | 36/6 |
As can be seen from Table 1, bacillus subtilis CM-A12 has the strongest product amylase, protease and cellulase ability,
The transparent circle diameter of three kinds of enzymes and colony diameter ratio are all more than 4.0.
The qualification of embodiment 2 sporeformer CM-A12
(1) morphological characteristic
After bacterial strain CM-A12 cultivates 24 hours on the foster base of solid LB growth, bacterium colony transparent globule shape, protrusion of surface, wet
Moisten glossy；After cultivating 48h, bacterium colony dry tack free, in rice white.Basis of microscopic observation is brevibacterium to strain cell, as
Shown in Fig. 1.
(2) Physiology and biochemistry identifies (as shown in table 2)
Table 2
Illustrate: "+": reaction is positive；"-" reaction is negative.
(3) 16S rDNA measures
Not can determine that the kind of antibacterial by strain morphology observation of characteristics, the 16S rDNA gene order of bacterial strain checked order,
Carry out the qualification of molecular level.
The PCR amplification of a bacterial strain 16S rDNA
1. PCR amplification system:
Table 3PCR amplification system
| System | Content (μ l) |
| 2×MightyAmp Buffer Ver.2 | 30 |
| MightyAmp DNA Polymerase | 1.5 |
| DNTP primer 2 7F (2.5mmol/L) | 1.5 |
| DNTP primer 1492R (2.5mmol/L) | 1.5 |
| Template | Trace thalline |
| ddH2O | 25.5 |
| Total system | 60 |
2. PCR primer employing 16S rRNA bacterial universal primers:
27F:5 '-AGA GTT TGA TCM TGG CTC AG-3 ' (SEQ ID NO.2)
1492R:5 '-GGY TAC CTT GTT ACG ACTT-3 ' (SEQ ID NO.3)
3. PCR amplification condition:
Acquire the 16S rDNA fragment of a length of 1379bp through order-checking, the number of registration in GenBank is
KT891015, base sequence is as shown in example table 3.
The 16S rDNA sequence of bacterial strain CM-A12 and GenBank related data carry out BLAST sequence analysis analysis, send out
The 16S rDNA sequence of existing bacterial strain CM-A12 and the Bacillus subtilis in known array in GenBank data base
LL-16 (KF683170) sequence similarity is up to 99%, chooses the reference culture that 10 strain sequence similarity rates are high, uses Neighbor
The phylogenetic tree that Joining (NJ) achievement method builds as in figure 2 it is shown, in constructed growth tree, bacterial strain CM-A12
It is also in same branch with Bacillus subtilis LL-16 (KF683170).
Combining form feature, Physiology and biochemistry are identified and molecule 16S rDNA identifies, determine that this bacterium is bacillus subtilis (Bacillus
Subtilis), by its named bacillus subtilis (Bacillus subtilis) CM-A12, and it is military that this bacterial strain is delivered to be positioned at China
The China typical culture collection center (being called for short CCTCC) of Chinese Wuhan University carries out preservation, and deposit number is CCTCC NO:M
2015688, preservation date is on November 23rd, 2015.
Embodiment 3 bacillus subtilis (Bacillus subtilis) CM-A12 growth conditions optimizes
Strain cultured solution: peptone 10g/L, Carnis Bovis seu Bubali cream 5g/L, NaCl 5g/L, pH 7～7.2.
(1) invention bacterial strain the most suitable growth pH
With HCl and NaOH of 1.0mol/L the initial pH of culture fluid is adjusted to respectively 4.0,5.0,6.0,7.0,8.0,9.0,
10.0,11.0, by 1% (v/v) inoculum concentration access CM-A12,30 DEG C, 150r/min cultivate, respectively cultivate 20h, 40
After h, at 600nm, survey its absorbance with ultraviolet-uisible spectrophotometer, using the culture fluid do not inoculated as blank.
Known to Fig. 3, initial pH is between 4～9, and CM-A12 well-grown, pH growth scope is wide；In original ph
It is little to growth for bacterial strain CM-A12 during more than 9.0.
Economically considering, CM-A12 is when a large amount of production fermentation, and culture medium is nature pH；Raw being added to waste water
When thing processes application in engineering, water body pH, in the range of 4～9, reduces procedure of pH adjustment, reduces cost for wastewater treatment.
(2) invention bacterial strain optimum growth temperature
In culture fluid by 1% (v/v) inoculum concentration access CM-A12, be individually positioned in 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C,
37, in 40 DEG C of shaking table incubators, 150r/min cultivates, and respectively after cultivating 20h, 40h, uses ultraviolet-uisible spectrophotometer
Its absorbance is surveyed at 600nm.
As seen from Figure 4, temperature is the key factor affecting CM-A12 growth.CM-A12 is equal in 20～40 DEG C
Can grow, in 20～37 DEG C of temperature ranges, the highest more beneficially bacterial strain the growth of temperature.The most suitable growth of bacterial strain CM-A12
Temperature is 37～40 DEG C.
(3) salinity impact on strain growth
Respectively preparation salinity be 0.0%, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%,
10.0%, 11.0%, 12.0%, 13.0%, 14.0%, 15.0%, culture fluid, access the bacterial strain CM-A12 of 1% (v/v),
In 30 DEG C, 150r/min constant temperature culture, after 20h, 40h, at 600nm, survey its extinction with ultraviolet-uisible spectrophotometer
Degree.
As shown in Figure 5, in invention, bacterial strain CM-A12 can grow in salt concentration range 0.0～14.0%, belongs to moderate salt tolerant
Bacterium, but in 1.0% salt concentration range, the growth of bacterial strain CM-A12 is the fastest.
Embodiment 4 bacillus subtilis (Bacillus subtilis) CM-A12 is applied to industrial park composite waste
(1) using polyurethane sponge filler to carry out bio-film colonization as bio-carrier, filler specification is 2cm × 2cm × 2cm；
Loading sponge filler, growth medium in 1L conical flask, solid-liquid ratio is 3:10, by bacillus subtilis (Bacillus subtilis)
CM-A12 is inoculated in growth medium, and in 37 DEG C of shaking table incubators, 150r/min cultivates and filler carried out biofilm, then in 3 days
Displace culture fluid with isopyknic composite waste, so after domestication 5 days, add composite waste, every day sampling and measuring ammonia nitrogen,
COD。
(2) experimental result is as shown in Figure 6, and ammonia nitrogen, COD are had by bacillus subtilis (Bacillus subtilis) CM-A12
Well degradation capability, is down to 345mg/L, clearance 71% through the COD of strains A 12 processed waste water by 1206mg/L；
After process, ammonia nitrogen is down to 3.01mg/L, clearance 88% by 25.5mg/L.Visible bacillus subtilis (Bacillus subtilis)
CM-A12 has good water purification effect to the purification of waste water.
Embodiment 5 bacillus subtilis (Bacillus subtilis) CM-A12 is applied to dyeing waste water
(1) use embedding techniques to fix bacillus subtilis (Bacillus subtilis) CM-A12, make biological fixnig microsphere.
Embedded material is polyvinyl alcohol, sodium alginate, SiO 2 powder and calcium carbonate, and adds iron powder, concrete embedding method reference
Document: Min Hang, Zheng Yaotong, Qian Zepeng, Chen Meici, polyvinyl alcohol embedding anaerobic activated sludge processes the optimal condition of waste water
Research, environmental science, 1994,15 (5)；
(2) with dyeing waste water for processing object, loading immobilized microspheres, dyeing waste water in 1L conical flask, solid-liquid ratio is 3:
10, it is positioned in 150r/min, 37 DEG C of shaking table incubators, certain time sampling and measuring ammonia nitrogen, COD.
(3) before treatment of dyeing wastewater, waste water darker in color is deep, in aubergine；After administering after microsphere processes, water quality is the limpidest, slightly
Band yellow, CODcr clearance more than 60%, ammonia nitrogen removal reaches more than 95%.
Embodiment 6 bacillus subtilis (Bacillus subtilis) CM-A12 is applied to community pollution of river water
(1) using polyurethane sponge filler to carry out bio-film colonization as bio-carrier, filler specification is 5cm × 5cm × 5cm；
Loading sponge filler, growth medium in 5L reaction vessel, solid-liquid ratio is 3:10, by bacillus subtilis (Bacillus
Subtilis) CM-A12 is inoculated in growth medium, room temperature 25～35 DEG C, DO cultivate between 2.0～3.0mg/L 5 days right
Filler carries out biofilm.Then with community, Huzhou pollution of river water for processing object, flow is 0.9L/h, and hydraulic detention time is equal
For 12h, after three days, every day sampling and measuring ammonia nitrogen, COD.
(2) result shows, community pollution of river water through bacillus subtilis (Bacillus subtilis) CM-A12 process after,
The COD of river is down to 30～45mg/L by 80～90mg/L, and clearance reaches more than 50%；After process, ammonia nitrogen is by 15～30mg/L
Being down to 1～4.5mg/L, clearance reaches more than 85%.Visible bacillus subtilis (Bacillus subtilis) CM-A12 is to river
Road water body has good repair.
Claims (10)
1. a bacillus subtilis strain, it is characterised in that named bacillus subtilis (Bacillus subtilis) CM-A12,
Deposit number is CCTCC NO:M 2015688, and preservation date is on November 23rd, 2015.
2. bacillus subtilis strain as claimed in claim 1, it is characterised in that described bacillus subtilis (Bacillus
Subtilis) the 16S rDNA sequence of CM-A12 is as shown in SEQ ID NO.1.
3. the cultural method of a bacillus subtilis strain, it is characterised in that by the bacillus subtilis described in claim 1
Bacterial strain activation after, be inoculated into original ph be 4～9, temperature be 25～40 DEG C, the culture medium that mass concentration is 0～14% of salt
Middle cultivation.
4. the bacillus subtilis strain as claimed in claim 1 application in water remediation or waste water process.
Apply the most as claimed in claim 1, it is characterised in that described water body is river course or lake；Described waste water is life dirt
Water, breeding wastewater, dyeing waste water, food service industry waste water or paper waste.
6. the application as described in claim 4 or 5, it is characterised in that including: described bacillus subtilis is directly added
Biochemical treatment is carried out to water body or waste water；Or after immobilization, then water body or waste water are carried out biochemical treatment.
7. the microbial inoculum of the bacillus subtilis strain comprised described in claim 1.
8. the microbe microsphere of the bacillus subtilis strain comprised described in claim 1.
9. the microbial inoculum as claimed in claim 7 application in water remediation or waste water process.
10. the microbe microsphere as claimed in claim 8 application in water remediation or waste water process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610625978.6A CN106011034B (en) | 2016-08-02 | 2016-08-02 | One bacillus subtilis strain and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610625978.6A CN106011034B (en) | 2016-08-02 | 2016-08-02 | One bacillus subtilis strain and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106011034A true CN106011034A (en) | 2016-10-12 |
| CN106011034B CN106011034B (en) | 2019-07-30 |
Family
ID=57134214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610625978.6A Active CN106011034B (en) | 2016-08-02 | 2016-08-02 | One bacillus subtilis strain and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106011034B (en) |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967646A (en) * | 2017-04-27 | 2017-07-21 | 佛山科学技术学院 | A kind of high temperature-resistant acid-resistant and the quick bacillus subtilis of growth and its application |
| CN107354112A (en) * | 2017-08-23 | 2017-11-17 | 江苏南资环保股份有限公司 | One plant of thiophene worm aldehyde degradation bacteria and its application |
| CN107446842A (en) * | 2017-07-24 | 2017-12-08 | 常州市萤火虫生物科技有限公司 | One bacillus subtilis and its application in purifying water |
| CN107460148A (en) * | 2017-09-01 | 2017-12-12 | 湖州至美生物科技有限公司 | A kind of preparation method and application of bacillus subtilis bacterium powder |
| CN108587978A (en) * | 2018-05-21 | 2018-09-28 | 黑龙江东方学院 | The bacillus subtilis of one plant of production alkali protease |
| CN109182210A (en) * | 2018-10-15 | 2019-01-11 | 青岛农业大学 | A kind of bacterial strain for sewage treatment |
| CN109266583A (en) * | 2018-10-16 | 2019-01-25 | 青岛农业大学 | A kind of sewage treatment bacterial product using oyster shell as carrier |
| CN109679872A (en) * | 2019-01-09 | 2019-04-26 | 武汉设计工程学院 | The Bacillus subtillis CP-3 separated from cray enteron aisle |
| CN110438024A (en) * | 2019-04-17 | 2019-11-12 | 浙江亲水园生物科技有限公司 | A kind of bacillus subtilis strain and its application |
| CN112322526A (en) * | 2020-10-30 | 2021-02-05 | 中国热带农业科学院热带生物技术研究所 | Bacillus south China sea with cellulose hydrolysis effect and application thereof |
| CN112375720A (en) * | 2020-12-17 | 2021-02-19 | 洛阳师范学院 | Bacillus subtilis and application thereof |
| CN112553112A (en) * | 2020-12-19 | 2021-03-26 | 武汉水之国环保科技有限公司 | Denitrifying bacillus subtilis and application thereof in strain detoxification |
| CN112574918A (en) * | 2020-12-17 | 2021-03-30 | 山东省科学院生物研究所 | Ammonia nitrogen degrading bacteria, microbial agent and application thereof |
| CN112646742A (en) * | 2020-12-22 | 2021-04-13 | 武汉水之国环保科技有限公司 | Activated sludge culture agent and application thereof |
| CN112744929A (en) * | 2020-12-19 | 2021-05-04 | 武汉水之国环保科技有限公司 | Application of bacillus subtilis SZG-JD-001 in preparation of biological composite carbon source |
| CN112939240A (en) * | 2021-02-09 | 2021-06-11 | 谷尚昆 | Method for treating sewage by using bacillus |
| CN113249273A (en) * | 2021-06-30 | 2021-08-13 | 泸州老窖股份有限公司 | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment |
| CN113583890A (en) * | 2021-06-03 | 2021-11-02 | 南京雷德蒙德农业科技有限公司 | Bacillus subtilis strain and application thereof |
| CN114480566A (en) * | 2022-03-04 | 2022-05-13 | 河南金百合生物科技股份有限公司 | Method for detecting whether bacterial strain produces enzyme |
| CN115287212A (en) * | 2022-04-14 | 2022-11-04 | 国家林业和草原局竹子研究开发中心 | Salt-tolerant growth-promoting bacterium ACP81 and application thereof |
| CN115353987A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof |
| CN117070428A (en) * | 2023-10-17 | 2023-11-17 | 北京大北农科技集团股份有限公司 | Application of bacillus subtilis BS-22 strain in improving cultivation environment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103013874A (en) * | 2012-12-13 | 2013-04-03 | 中国地质大学（武汉） | Bacillus subtilis ds3 |
| CN103748214A (en) * | 2011-07-13 | 2014-04-23 | 生物地带有限公司 | Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same |
| CN104164391A (en) * | 2014-07-09 | 2014-11-26 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis and application thereof in aquaculture |
-
2016
- 2016-08-02 CN CN201610625978.6A patent/CN106011034B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103748214A (en) * | 2011-07-13 | 2014-04-23 | 生物地带有限公司 | Biological product for clearing of water, industrial wastewater and soil from chemicals resistant to degradation, and method for using the same |
| CN103013874A (en) * | 2012-12-13 | 2013-04-03 | 中国地质大学（武汉） | Bacillus subtilis ds3 |
| CN104164391A (en) * | 2014-07-09 | 2014-11-26 | 青岛玛斯特生物技术有限公司 | Bacillus subtilis and application thereof in aquaculture |
Non-Patent Citations (5)
| Title |
|---|
| 丁祥力: "淡水养殖水体净化菌的分离鉴定及其应用特性的研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 施安辉等: "高效水质净化剂菌种的选育、制剂研制及应用", 《山东食品发酵》 * |
| 汪文俊: "枯草芽孢杆菌对不同富营养化水体的净化作用", 《湖北农业科学》 * |
| 胡咏梅等: "枯草芽孢杆菌FY99-01菌株的净水作用", 《华中农业大学学报》 * |
| 陈尚智: "枯草芽孢杆菌的固定及其对微污染水体的净化研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967646A (en) * | 2017-04-27 | 2017-07-21 | 佛山科学技术学院 | A kind of high temperature-resistant acid-resistant and the quick bacillus subtilis of growth and its application |
| CN107446842A (en) * | 2017-07-24 | 2017-12-08 | 常州市萤火虫生物科技有限公司 | One bacillus subtilis and its application in purifying water |
| CN107446842B (en) * | 2017-07-24 | 2020-07-07 | 常州市萤火虫生物科技有限公司 | Bacillus subtilis and application thereof in water purification |
| CN107354112A (en) * | 2017-08-23 | 2017-11-17 | 江苏南资环保股份有限公司 | One plant of thiophene worm aldehyde degradation bacteria and its application |
| CN107354112B (en) * | 2017-08-23 | 2020-08-14 | 江苏南资环保科技有限公司 | Thioanal degrading bacterium and application thereof |
| CN107460148A (en) * | 2017-09-01 | 2017-12-12 | 湖州至美生物科技有限公司 | A kind of preparation method and application of bacillus subtilis bacterium powder |
| CN108587978A (en) * | 2018-05-21 | 2018-09-28 | 黑龙江东方学院 | The bacillus subtilis of one plant of production alkali protease |
| CN109182210A (en) * | 2018-10-15 | 2019-01-11 | 青岛农业大学 | A kind of bacterial strain for sewage treatment |
| CN109182210B (en) * | 2018-10-15 | 2021-05-07 | 青岛农业大学 | Bacterial strain for sewage treatment |
| CN109266583A (en) * | 2018-10-16 | 2019-01-25 | 青岛农业大学 | A kind of sewage treatment bacterial product using oyster shell as carrier |
| CN109266583B (en) * | 2018-10-16 | 2021-05-25 | 青岛农业大学 | Sewage treatment bacterium product using oyster shell as carrier |
| CN109679872A (en) * | 2019-01-09 | 2019-04-26 | 武汉设计工程学院 | The Bacillus subtillis CP-3 separated from cray enteron aisle |
| CN110438024A (en) * | 2019-04-17 | 2019-11-12 | 浙江亲水园生物科技有限公司 | A kind of bacillus subtilis strain and its application |
| CN110438024B (en) * | 2019-04-17 | 2023-03-28 | 浙江亲水园生物科技有限公司 | Bacillus subtilis strain and application thereof |
| CN112322526A (en) * | 2020-10-30 | 2021-02-05 | 中国热带农业科学院热带生物技术研究所 | Bacillus south China sea with cellulose hydrolysis effect and application thereof |
| CN112375720B (en) * | 2020-12-17 | 2023-06-16 | 洛阳师范学院 | Bacillus subtilis and application thereof |
| CN112574918A (en) * | 2020-12-17 | 2021-03-30 | 山东省科学院生物研究所 | Ammonia nitrogen degrading bacteria, microbial agent and application thereof |
| CN112375720A (en) * | 2020-12-17 | 2021-02-19 | 洛阳师范学院 | Bacillus subtilis and application thereof |
| CN112744929A (en) * | 2020-12-19 | 2021-05-04 | 武汉水之国环保科技有限公司 | Application of bacillus subtilis SZG-JD-001 in preparation of biological composite carbon source |
| CN112553112A (en) * | 2020-12-19 | 2021-03-26 | 武汉水之国环保科技有限公司 | Denitrifying bacillus subtilis and application thereof in strain detoxification |
| CN112744929B (en) * | 2020-12-19 | 2022-08-05 | 武汉水之国环保科技有限公司 | Application of bacillus subtilis SZG-JD-001 in preparation of biological composite carbon source |
| CN112646742B (en) * | 2020-12-22 | 2022-06-24 | 武汉水之国环保科技有限公司 | Activated sludge culture agent and application thereof |
| CN112646742A (en) * | 2020-12-22 | 2021-04-13 | 武汉水之国环保科技有限公司 | Activated sludge culture agent and application thereof |
| CN112939240B (en) * | 2021-02-09 | 2023-02-24 | 谷尚昆 | Method for treating sewage by using bacillus |
| CN112939240A (en) * | 2021-02-09 | 2021-06-11 | 谷尚昆 | Method for treating sewage by using bacillus |
| CN113583890A (en) * | 2021-06-03 | 2021-11-02 | 南京雷德蒙德农业科技有限公司 | Bacillus subtilis strain and application thereof |
| CN113249273B (en) * | 2021-06-30 | 2022-03-08 | 泸州老窖股份有限公司 | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment |
| CN113249273A (en) * | 2021-06-30 | 2021-08-13 | 泸州老窖股份有限公司 | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment |
| CN115353987A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof |
| CN115353987B (en) * | 2022-01-28 | 2023-07-07 | 齐齐哈尔大学 | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof |
| CN114480566A (en) * | 2022-03-04 | 2022-05-13 | 河南金百合生物科技股份有限公司 | Method for detecting whether bacterial strain produces enzyme |
| CN115287212A (en) * | 2022-04-14 | 2022-11-04 | 国家林业和草原局竹子研究开发中心 | Salt-tolerant growth-promoting bacterium ACP81 and application thereof |
| CN117070428A (en) * | 2023-10-17 | 2023-11-17 | 北京大北农科技集团股份有限公司 | Application of bacillus subtilis BS-22 strain in improving cultivation environment |
| CN117070428B (en) * | 2023-10-17 | 2024-02-27 | 北京大北农科技集团股份有限公司 | Application of bacillus subtilis BS-22 strain in improving cultivation environment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106011034B (en) | 2019-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106011034B (en) | One bacillus subtilis strain and its application | |
| Apun et al. | Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste | |
| Thippeswamy et al. | Isolation and identification of α-amylase producing Bacillus sp. from dhal industry waste | |
| Vaseekaran et al. | Isolation and identification of a bacterial strain producing thermostable α-amylase. | |
| CN104560816B (en) | Bacillus licheniformis with biomass hydrolase activity and application thereof | |
| CN103320356B (en) | Protease-producing strain exiguobacterium sp. and applications thereof | |
| CN105950495B (en) | One plant of Methylotrophic bacillus and its application in livestock breeding wastewater processing | |
| CN101245327B (en) | Bacillus licheniformis and method for producing alkali proteinase with sewage sludge as raw material | |
| CN106399156B (en) | Bacillus amyloliquefaciens and application thereof in gulfweed biodegradation | |
| CN102399726B (en) | Sporosarcina and application thereof | |
| Ayuningrum et al. | Screening of actinobacteria-producing amylolytic enzyme in sediment from Litopenaeus vannamei (Boone, 1931) ponds in Rembang District, Central Java, Indonesia | |
| CN102703420A (en) | Microbial degradation preparation for municipal garbage and sewage and preparation method thereof | |
| CN112646741B (en) | Bacillus subtilis subspecies desertae S2 and application thereof | |
| CN101245325B (en) | Stenotrophomonas maltophilia of strain producing novel alkali proteinase | |
| CN105199983B (en) | The application of one bacillus amyloliquefaciens and its protease of generation in terms of process hides depilation | |
| CN108239612B (en) | Neisseri (Nesterenkonia) strain X35 and application thereof | |
| CN112574918B (en) | Ammonia nitrogen degrading bacteria, microbial agent and application thereof | |
| CN101544958A (en) | High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application | |
| KR101108472B1 (en) | Bacillus subtilis cbs strain and direct-fed microorganisms comprising the same | |
| Nagvenkar et al. | Microbial diversity and enzyme production in mullet Mugil cephalus L.(Pisces) along Goa, west coast of India | |
| CN101407778A (en) | Spherical bacillus and organic solvent-resistant stable proteinase produced thereby | |
| CN102965297B (en) | Cellulose-degrading Escherichia coli | |
| CN114437961B (en) | Bacillus amyloliquefaciens composite microbial agent for reducing water surface tension and application thereof | |
| CN110791446B (en) | Ecological toilet treatment strain and application thereof | |
| De et al. | An insight into the celluloytic potential of three strains of Bacillus spp. isolated from benthic soil of aquaculture farms in East Kolkata Wetlands, India. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |