CN107354112B - Thioanal degrading bacterium and application thereof - Google Patents
Thioanal degrading bacterium and application thereof Download PDFInfo
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- CN107354112B CN107354112B CN201710729727.7A CN201710729727A CN107354112B CN 107354112 B CN107354112 B CN 107354112B CN 201710729727 A CN201710729727 A CN 201710729727A CN 107354112 B CN107354112 B CN 107354112B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/04—Pesticides, e.g. insecticides, herbicides, fungicides or nematocides
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- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
Abstract
The invention discloses a thiacloprid aldehyde degrading bacterium and application thereof, wherein the degrading bacterium is identified as bacillus subtilisBacillus subtilisBelongs to gram-positive bacteria, which are preserved in China general microbiological culture Collection center (CGMCC) at 27.5.2017, and the preservation number is as follows: CGMCC NO.14200 can grow by taking thiacloprid as the only carbon source and energy source, has a degradation rate of more than 90% to 300 mg/L thiacloprid within 48 h, and can be applied to treatment of wastewater and sewage left by thiacloprid or polluted soil remediation.
Description
Technical Field
The invention belongs to the technical field of biodegradation bacteria, and particularly relates to thiacloprid aldehyde degrading bacteria and application thereof.
Background
China is a big country for producing and using pesticides, the annual pesticide use amount is about 80 ten thousand tons, the country is the first place in the world, and the total pesticide production amount is famous for the second place in the world from 1990 and is only second to the United states. At present, the structure of the pesticide product in China still mainly takes the pesticide as the main component, the heterocyclic pesticide with the advantages of super high efficiency, unique action mechanism, wide insecticidal spectrum, good environmental compatibility and the like becomes a new key point for developing and developing the pesticide, and the production capacity of the pesticide accounts for about 70 percent of the total production capacity of the pesticide. Due to the excellent control effect, the pesticide composition becomes a new choice and an important means for replacing high-toxicity and high-residue pesticides to control pests. In order to increase the yield and prevent diseases and insect pests, pesticide plays an important role in China for a long time, however, the popularization and the use of the pesticide have immeasurable influence on the living environment of people, particularly on soil, and the basic normal function of farming is lost due to the fact that the soil fertility is reduced in some areas because of abuse or excessive use of the pesticide. The adsorption-desorption effect of the soil on the pesticide can seriously affect the activities of the pesticide such as migration, transformation and the like in the soil.
Thiacloprid has the characteristics of stomach toxicity, contact killing, systemic property and the like, is effective on a plurality of pests, particularly piercing-sucking mouthpart pests, and has been widely applied to control hemiptera, coleopteran, certain lepidopteran and other pests on crops such as rice, fruit trees, cotton, tea leaves, turf, ornamental plants and the like. The pesticide has long residual time in water environment, complex metabolic pathway and no negligence about the safety problem of agricultural products and environment.
Microorganisms have the characteristics of rapid propagation, rapid mutation, various metabolic pathways and the like, and microbial remediation is considered to be an effective means for pollutant remediation. The method is applied in many aspects, so that the screening of the thiacloprid aldehyde degrading bacteria has great significance for treating the waste water and soil with the thiacloprid aldehyde residue.
Disclosure of Invention
The invention aims to provide a thiacloprid aldehyde degrading bacterium and application thereof, the bacterium agent can enable the thiacloprid aldehyde degrading rate to reach more than 90 percent, and the production and use cost is lower.
In order to achieve the purpose, the invention adopts the technical scheme that:
a thiacloprid aldehyde degrading bacterium, strain NZJ-37 is a gram-positive bacterium, and has the main biological characteristics that the bacterium is oval to cylindrical, the edge is smooth, the stain is white or yellowish, and the diameter is 1-2 mm. It has been preserved in China culture collection in 2017 at 27.5 monthsGeneral microbiological center of the regulatory commission (CGMCC), address: xilu No.1 Hospital No. 3, Beijing, Chaoyang, on Beijing, with a deposit number: CGMCC NO.14200, identified as Bacillus subtilisBacillus subtilisThe thiacloprid aldehyde can be used as a unique carbon source and a unique nitrogen source for growth, and is mineralized to release ammonia nitrogen.
A microbial inoculum comprising said bacillus subtilis NZJ-37.
The method for producing the microbial inoculum by the bacillus subtilis NZJ-37 comprises the following specific steps:
1) inoculating a test tube seed of a thiacloprid aldehyde degrading bacterium bacillus subtilis NZJ-37 into an LB culture medium, and performing shaking culture until the logarithmic phase, wherein the LB culture medium formula is as follows: 10.00g/L of NaCl, 10.00g/L of peptone, 5.00g/L of yeast powder and pH 7.0;
2) inoculating the strains cultured in the step 1) into a 500L seeding tank according to the inoculation amount of 10%, culturing to the logarithmic phase, wherein the formula of a culture medium used by the seeding tank is as follows: glucose 0.8%, (NH)4）2SO41%，K2HPO40.2%，MgSO40.05%，NaCl0.01%，CaCO30.3 percent, 0.02 percent of yeast extract and 7.2 to 7.5 of pH value.
3) Inoculating the seed solution obtained in the step 2) into a production tank according to the inoculation amount of 10% for fermentation culture, wherein the culture medium used by the production tank is the same as that of the seed tank, and the culture solution after fermentation is the microbial inoculum.
In the culture process of the seeding tank in the step 2) and the production tank in the step 3), the ventilation volume of sterile air is 1: 0.6-1.2, stirring speed of 180-240r/min, culture temperature of 30-35 ℃, culture time of the whole process flow of the steps 2 and 3 of 48-60 h, the number of the thalli after fermentation is over 10 hundred million/mL, and the culture solution is directly taken out of a tank and is packaged into liquid agents by a plastic packaging barrel or a packaging bottle or prepared into solid adsorbents.
The thiacloprid aldehyde degrading bacterium or the application of the microbial inoculum in degrading the thiacloprid aldehyde.
The thiacloprid aldehyde degrading bacteria or the microbial inoculum is applied to the remediation of the waste water or soil with the thiacloprid aldehyde residue. The thiacloprid aldehyde degrading bacteria or the microbial inoculum is directly added into the waste water or soil containing the thiacloprid aldehyde residue, and the bacterial strain can grow by taking the thiacloprid aldehyde as a unique carbon source and a unique nitrogen source and mineralize the thiacloprid aldehyde to release ammonia nitrogen so as to achieve the purpose of degrading the thiacloprid aldehyde.
Has the advantages that:
the degradation rate of the bacillus subtilis NZJ-37 on thiacloprid aldehyde reaches over 90 percent, the workload in the production and use processes is greatly reduced, and the production and use costs are reduced. The invention has important significance for protecting the ecological environment and reducing the cost of wastewater treatment. The prepared microbial inoculum has the advantages of low production and use cost, convenient use and good removal effect. The degrading bacteria NZJ-37 can grow on a culture medium which takes thiacloprid as a unique carbon source; degrading bacteria NZJ-37 are inoculated into an inorganic salt culture medium containing thiacloprid according to the inoculation amount of 1 percent, and the degradation rate of the thiacloprid with a certain concentration in the wastewater can reach more than 90 percent after the oscillating culture is carried out for 48 hours at the temperature of 30 ℃ and the pH value of 7.0, thereby having good degradation effect.
Drawings
FIG. 1 is a colony morphology of Bacillus subtilis NZJ-37;
FIG. 2 Shake flask degradation plot of Bacillus subtilis NZJ-37.
Detailed Description
Example 1 isolation and characterization of the strains
Preparing a soil sample polluted by the thiacloprid into suspension, adding 10mL of the suspension into 100mL of basal salt culture medium with the final concentration of the thiacloprid of 300 mg/L, and carrying out enrichment culture at 30 ℃ and 160 r/min. Transferring the cultured strain into a new thiacloprid aldehyde inorganic salt culture medium with the inoculation amount of 10 percent, and continuously transferring for 4 times to obtain the enrichment solution. 1.0 mL of the enrichment solution is prepared into 10-1The enriched liquid is then sucked with 1.0 ml of prepared 10-1Adding the enriched solution into 9.0 ml of sterile water, and fully and uniformly mixing to prepare 10-2The enrichment solution is subjected to gradient dilution by analogy. 0.1 ml of each gradient of the dilution was pipetted and spread on a solid medium containing 300 mg/L of thiacloprid as inorganic salt (formulation: 1.50 g K/L)2HPO4、0.50 g KH2PO4、0.20 g MgSO4·7H2O、1.00 g NaCl、1.00 g (NH4)2SO420.00 g agar, pH 7.0), and cultured at 30 ℃ for 7 days. And selecting a single colony from the bacterial strains to verify the degradation effect, storing a strain with higher degradation efficiency, and performing subsequent experiments. The colony pattern is shown in FIG. 1 and identified as Bacillus subtilisBacillus subtilis. The main biological characteristics are that the thallus is elliptical to cylindrical, the edge is smooth, the thallus is white or yellowish, and the diameter is 1-2 mm.
Example 2 Shake flask degradation experiment of Bacillus subtilis NZJ-37
In a culture medium containing 300 mg/L of thiacloprid aldehyde inorganic salt, the formula is as follows: each liter of the culture medium contains 1.50 g K2HPO4, 0.50gKH2PO4, 0.20g MgSO4 & 7H2O, 1.00g NaCl, 1.00g (NH4)2SO4 and pH 7.0, NZJ-37 is inoculated according to the inoculation amount of 1 percent, the culture medium is shaken at 30 ℃, and sampling and determination are carried out at intervals of 6 hours. As can be seen from FIG. 2, thiacloprid aldehyde is degraded by more than 90% within 48 h.
Example 3 preparation of Bacillus subtilis NZJ-37-containing microbial inoculum
Stock strains of Bacillus subtilis NZJ-37 screened and isolated in example 1 were activated on petri dishes and tested for degradation and plated on tube slants for use. The test-tube strains are inoculated in a 1000ml shake flask containing 200ml LB culture medium, and the formula of the LB culture medium is as follows: 5.00g/L of yeast extract, 10.00g/L of peptone, 10.00g/L of NaCl and 7.0 of pH, carrying out constant-temperature shaking culture until the logarithmic phase, and preparing an inoculation seeding tank. 500 liters of seeding tank and 400 liters of batch size, and the formula of the culture medium is as follows: glucose 0.8%, (NH)4）2SO41%，K2HPO40.2%，MgSO40.05%，NaCl 0.01%， CaCO30.3 percent, 0.02 percent of yeast extract and 7.2 to 7.5 of pH value. After the feeding is finished, high-pressure moist heat sterilization is carried out at 121 ℃, after the temperature is cooled to 33 ℃, the cultured shake flask strain is inoculated into a 500-liter seeding tank according to the inoculation amount of 10 percent, the strain is cultured to the logarithmic phase, the stirring speed is 220 r/min, and the introduction amount of sterile air is 1: 0.8. Inoculating the seed liquid reaching logarithmic phase into a production tank according to the inoculation amount of 10% for culture, wherein the culture medium composition of the production tank is the same as that of a seed tank. The capacity of the production tank is 5 tons, and the feeding amount is 4.5 tons. 1.1kg/cm production tank after feeding2Sterilizing at 121 deg.C, cooling to below 35 deg.C, introducing sterile air to maintain sterilityThe state is standby. The temperature of the production tank after inoculation is controlled at 35 ℃, the ventilation quantity of sterile air in the culture process of the production tank is 1: 1.2, the stirring speed is 240r/min, and the culture time of the whole process flow is 60 hours. The number of the thalli after fermentation is over 10 hundred million/ml. After fermentation, the culture solution is directly taken out of the tank and is subpackaged into liquid dosage forms by using a plastic packaging barrel or a packaging bottle or into solid microbial inoculum dosage forms by adopting a packaging bag for peat adsorption.
Example 4 soil remediation test
Uniformly spraying 300 mg/L thiacloprid aqueous solution on 300g of sterilization test soil, inoculating 30 mL of microbial inoculum into the test soil, uniformly mixing, adding sterile water to adjust the water content of the soil, culturing the test soil at 30 ℃, taking an un-inoculated part as a blank control, sampling every 6 h to determine the thiacloprid residual quantity, and applying the microbial inoculum for 48 h to ensure that the thiacloprid degradation rate reaches more than 90%.
Claims (7)
1. A strain of thiacloprid aldehyde degrading bacteria, which is classified and named as bacillus subtilis (Bacillus subtilis)Bacillus subtilis) NZJ-37, which is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms with the preservation number: CGMCC NO. 14200.
2. The use of the thiacloprid aldehyde degrading bacteria of claim 1 to degrade thiacloprid aldehyde.
3. The use of the thiacloprid aldehyde degrading bacteria of claim 1 to remediate wastewater or soil having thiacloprid aldehyde residues.
4. A microbial agent comprising the thiacloprid aldehyde degrading bacterium of claim 1.
5. The method for preparing the microbial inoculum according to claim 4, which comprises the following steps:
step 1): inoculating the test tube species of the thiacloprid aldehyde degrading bacteria into an LB culture medium, and carrying out shake culture to logarithmic phase;
step 2): inoculating the cultured strain in the step 1) into a 500L seeding tank according to the inoculation amount of 10 percent, culturing to logarithmic phase,
step 3): inoculating the seed solution obtained by the seed tank culture in the step 2) into a production tank for culture according to the inoculation amount of 10 percent;
the ventilation of sterile air during the cultivation process of the seeding tank and the production tank is 1: 0.6-1.2, stirring speed of 180-.
6. The method according to claim 5, wherein the LB medium is formulated as follows: NaCl 10.00g/L, peptone 10.00g/L, yeast powder 5.00g/L, pH 7.0.
7. The method of claim 5, wherein the media formulation used in the seed tank and the production tank is: glucose 0.8%, (NH)4）2SO41%，K2HPO40.2%，MgSO40.05%，NaCl 0.01%，CaCO30.3 percent, 0.02 percent of yeast extract and 7.2 to 7.5 of pH value.
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| CN108441451B (en) * | 2018-04-12 | 2020-06-30 | 江苏世邦生物工程科技有限公司 | Composite microbial soil remediation agent and preparation method and application thereof |
| CN110172430B (en) * | 2019-06-18 | 2022-03-11 | 江苏省农业科学院 | Enterobacter mori and application thereof in degrading thiamethoxam |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4531002A (en) * | 1983-08-26 | 1985-07-23 | Shell Oil Company | Process for preparing insecticidal N-acyl-tetrahydro-2-nitromethylene-2H-1,3-thiazines |
| JPS63270607A (en) * | 1987-04-28 | 1988-11-08 | Shionogi & Co Ltd | Stabilized insecticidal composition and stabilization method |
| US4923987A (en) * | 1988-07-25 | 1990-05-08 | E. I. Du Pont De Nemours And Company | Process for the preparation of nitromethylene heterocyclic compounds |
| JPH04193806A (en) * | 1990-11-26 | 1992-07-13 | Shionogi & Co Ltd | Stable insecticide composition and stabilizing method |
| CN1302865A (en) * | 2001-01-17 | 2001-07-11 | 南京农业大学 | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation |
| CN106011034A (en) * | 2016-08-02 | 2016-10-12 | 浙江至美环境科技有限公司 | Bacillus subtilis strain and application thereof |
-
2017
- 2017-08-23 CN CN201710729727.7A patent/CN107354112B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4531002A (en) * | 1983-08-26 | 1985-07-23 | Shell Oil Company | Process for preparing insecticidal N-acyl-tetrahydro-2-nitromethylene-2H-1,3-thiazines |
| JPS63270607A (en) * | 1987-04-28 | 1988-11-08 | Shionogi & Co Ltd | Stabilized insecticidal composition and stabilization method |
| US4923987A (en) * | 1988-07-25 | 1990-05-08 | E. I. Du Pont De Nemours And Company | Process for the preparation of nitromethylene heterocyclic compounds |
| JPH04193806A (en) * | 1990-11-26 | 1992-07-13 | Shionogi & Co Ltd | Stable insecticide composition and stabilizing method |
| CN1302865A (en) * | 2001-01-17 | 2001-07-11 | 南京农业大学 | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation |
| CN106011034A (en) * | 2016-08-02 | 2016-10-12 | 浙江至美环境科技有限公司 | Bacillus subtilis strain and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| WL108477--a novel neurotoxic insecticide;Harris, M. et al.;《Brighton Crop Protection Conference-Pests and Diseases》;19861231;第1卷;第115-122页 * |
| 新的神经毒性杀虫剂——WL108477;M.Harris et al.;《农药译丛》;19901231;第12卷(第2期);第61-63,32页 * |
| 新颖神经毒性杀虫剂——噻虫醛;曹瑾等;《广西化工》;19971231;第26卷;第26-30,46页 * |
| 神经毒性杀虫剂WL108477;M.Harris et al.;《农药》;19871231(第5期);第46-48页 * |
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