CN113999795B - Burkholderia cepacia with deodorizing function and application thereof - Google Patents
Burkholderia cepacia with deodorizing function and application thereof Download PDFInfo
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- 241000589513 Burkholderia cepacia Species 0.000 title claims abstract description 49
- 230000001877 deodorizing effect Effects 0.000 title claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 32
- 230000004151 fermentation Effects 0.000 claims abstract description 32
- 239000010865 sewage Substances 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 7
- 238000009629 microbiological culture Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 229910021529 ammonia Inorganic materials 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 230000000813 microbial effect Effects 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 235000015278 beef Nutrition 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 235000019733 Fish meal Nutrition 0.000 claims description 4
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 4
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 4
- 240000008042 Zea mays Species 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 4
- 235000005822 corn Nutrition 0.000 claims description 4
- 239000004467 fishmeal Substances 0.000 claims description 4
- 235000013312 flour Nutrition 0.000 claims description 4
- 238000004321 preservation Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000001580 bacterial effect Effects 0.000 description 17
- 239000001963 growth medium Substances 0.000 description 17
- 239000002609 medium Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 7
- 102000004190 Enzymes Human genes 0.000 description 7
- 238000009630 liquid culture Methods 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 4
- 229930182566 Gentamicin Natural products 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 150000001299 aldehydes Chemical class 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 229960002518 gentamicin Drugs 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 108010093965 Polymyxin B Proteins 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 229920000024 polymyxin B Polymers 0.000 description 3
- 229960005266 polymyxin b Drugs 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 238000004887 air purification Methods 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000013587 production medium Substances 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 108010040201 Polymyxins Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The invention discloses burkholderia cepacia with a deodorizing function and application thereof, and belongs to the technical field of microorganisms. The strain is obtained by separating and purifying urban river sewage, is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center) for 04 month 23 years, has a preservation address of No. 1 and No. 3 of North Chen West Lu in the Korean area of Beijing city, has a preservation number of CGMCC No.22231, and is named as Burkholderia cepacia (Burkholderia cepacia) in taxonomy. The Burkholderia cepacia (Burkholderia cepacia) fermentation extract contains a large amount of deodorizing bacteria, and has obvious effects on deodorizing and purifying air.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to Burkholderia cepacia with a deodorizing function and application thereof.
Background
Burkholderia cepacia is a gram-negative bacillus widely existing in water, soil, plants and human bodies, and some strains in the bacillus have great economic and ecological values in aspects of biological control and sewage purification. Some of these strains have been found to degrade toxic and carcinogenic chemical components of pesticides and herbicides. Some countries in Europe and America use the strain as a biological pesticide by abandoning or limiting the use of the strain as a conditional pathogen, but at present, the biological pesticide prepared by using the strain as a raw material is produced and used in a plurality of countries to engage in agricultural production and pest control activities. China was allowed to register this type of product in 1996.
In recent years, burkholderia cepacia has shown good application prospects in agricultural production. However, the domestic application of Burkholderia cepacia is mostly only remained in agriculture, but the research on water purification is almost not performed.
Disclosure of Invention
The invention aims to provide a burkholderia cepacia with a deodorizing function, which is preserved in China general microbiological culture collection center (CGMCC) with a preservation number of 22231 and is named as burkholderia cepacia (Burkholderia cepacia) in 2021, 04 and 23.
The second object of the present invention is to provide a fermentation method of burkholderia cepacia using a seed medium of NA: 0.5% peptone, 0.3% beef extract, 0.5% sodium chloride, distilled water, and pH was adjusted to 7.0.
Preferably, the liquid fermentation medium used in the fermentation of burkholderia cepacia (Burkholderia cepacia) is: 15g of corn flour, 4g of glucose, 21g of bean cake powder, 4g of fish meal and CaCO 3 9g,(NH 4 ) 2 SO 4 1.5g,K 2 HPO 4 0.5g，MgSO 4 ·7H 2 O0.1 g, naC10.1g, 1000ml of water, and pH 7.5.
The third object of the present invention is to provide a microbial agent comprising Burkholderia cepacia (Burkholderia cepacia).
The fourth object of the present invention is to provide a fermentation broth obtained by the fermentation method of Burkholderia cepacia.
The fifth purpose of the invention is to provide the application of the burkholderia cepacia (Burkholderia cepacia) or the microbial agent containing the burkholderia cepacia in the ammonia removal of sewage.
The sixth object of the present invention is to provide an application of the Burkholderia cepacia (Burkholderia cepacia) or a microbial agent containing the same in removing aldehyde.
The seventh object of the invention is to provide the application of the fermentation method of Burkholderia cepacia in removing ammonia from sewage.
The invention aims at providing the application of the fermentation method of Burkholderia cepacia in formaldehyde removal of sewage.
Compared with the prior art, the invention has the following beneficial effects:
the invention successfully separates a deodorizing and purifying strain, fully utilizes the characteristic of the strain, and develops a deodorizing product of the microbial lysis extracting solution applied to environmental deodorization and atmosphere peculiar smell removal. The biological enzyme substances generated in the microbial fermentation process thoroughly decompose odor and smell, purify air and beautify environment.
Biological preservation description:
preservation mechanism: china general microbiological culture Collection center (China Committee for culture Collection);
preservation number: CGMCC No.22231;
preservation date: 2021, 04, 23;
preservation address: beijing, chaoyang area, north Chenxi Lu No. 1, 3;
taxonomic naming: burkholderia cepacia (Burkholderia cepacia).
Drawings
FIG. 1 is a phylogenetic tree of B.cepacia BBC-1 of the present invention.
Detailed Description
Example 1
The method for separating and purifying burkholderia cepacia comprises the following steps:
(1) 20 parts of water sample is taken from a Baoding sewage treatment plant and LB solid medium is prepared.
LB solid medium: 1% of tryptone, 0.5% of yeast powder, 1% of sodium chloride, 1.8% of agar, distilled water and regulating the pH value to 7.2.
20 water samples were subjected to plate streak culture at 37℃for 24 h.
(2) Single colony is picked and shake flask culture is carried out.
(1) Taking 4 conical flasks of 250ml, pouring 30ml of trypticase soy peptone liquid culture medium respectively, selectively picking colonies according to the form and state of bacteria on a flat plate, respectively inoculating into the conical flasks filled with the culture medium at 100rpm/min and 36 ℃, measuring OD value and pH value after shaking culture for 16 hours, and observing by a smear microscope.
(2) Preparing BCSA agar containing polymyxin B and gentamicin.
After autoclaving the agar, cooling to about 50 ℃, adding 2.4ml of filtered polymyxin B,1ml of filtered gentamicin, shaking up and pouring into a plate. Inoculating the cultured bacterial solutions in shake flasks, culturing at 36 ℃ for 24 hours, and observing the growth state of the bacterial colonies. The microscopic observation shows that the bacteria pollution is avoided, and the shake flask has good growth.
The tryptone liquid culture medium is a general enrichment culture medium, BCSA agar containing polymyxin B and gentamicin (containing polymyxin B60000U/100 ml and gentamicin 1mg/100 ml) is a selective culture medium of Burkholderia cepacia, has an inhibiting effect on other microorganisms (reference documents: gao Yecheng, yu Xiaoyi and Ni Qinggui. Burkholderia cepacia detection method [ J ].2014, annual meeting of the food society of Guangdong province, 2014, 251-255.) and is named as Burkholderia cepacia BBC-1 because the colony on the plate grows well through observation.
The strain morphology of burkholderia cepacia BBC-1 is observed: gram-negative rod-shaped cells with a plurality of extreme flagella, on TSA culture medium, colony is bright yellowish, raised, and the edge is neat to irregular; colonies on KMB medium were pale yellow green, non-fluorescent, and pale yellow green non-fluorescent diffuse pigments were produced on the medium.
The sequence of the 16sRNA sequence of the bacterium is as follows:
SEQ ID NO.1：
TGGGGAATCTTGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGAATGATGAAGGTCTTAGGATTGTAAAATTCTTTCACCGGGGACGATAATGACGGTACCCGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGTGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTAGGCGGACAGTTAAGTCGGGGGTGAAAGCCCGGGGCTCAACCTCGGAATTGCCTTCGATACTGGCTGTCTTGAGTACGGGAGAGGTGTGTGGGACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGACACACTGGCCCGTTACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGA。
example 2
The cultivation method of the burkholderia cepacia BBC-1 isolated and obtained in example 1 is as follows:
(1) And (5) seed culture.
NA medium: peptone 5g, beef extract 3g, naCl 5g, distilled water 1000ml, ph7.0.
20 mu L of strain is inoculated into 30ml of liquid culture medium, and cultured for 12 hours at 30 ℃ and 200r/min. Bacterial liquid is taken, OD600 and pH are detected.
(2) And (5) shaking and fermenting.
Initial enzyme production medium: peptone 5g, K 2 HPO 4 ：2g，MgSO 4 ·7H 2 O:0.05g, 1000ml of distilled water, pH7.0. Inoculating the seed bacterial liquid into the initial enzyme production culture medium, and culturing for 15h at 30 ℃ and 200r/min with the inoculum size of 0.5%.
(3) And (5) screening a carbon source.
The carbon source of the initial enzyme-producing medium was changed, 5 kinds of mediums were prepared according to Table 1, and the inoculated amount was 0.5%,30℃and 200r/min, and cultured for 15 hours.
TABLE 1
And (3) taking bacterial liquid, detecting OD600 and ph, and screening out an optimal carbon source according to bacterial concentration detection.
The results of the carbon source screening are shown in Table 2.
TABLE 2
| Primary culture medium | Glucose 5g | Glucose 7.5g | Starch 2.5g | Starch 10g | |
| Ph before sterilization | 7.03 | 7.03 | 7.04 | 7.02 | 7.02 |
| After sterilization Ph | 6.99 | 6.81 | 6.76 | 6.96 | 6.97 |
| OD | 1.0587 | 6.5412 | 1.7601 | 1.0069 | 0.7813 |
| Bacterial liquid ph | 7.78 | 4.85 | 4.06 | 7.72 | 7.65 |
(4) And (5) screening nitrogen sources.
And (5) continuously optimizing the nitrogen source according to the carbon source screened by the steps. 6 different culture media were prepared according to Table 3, the inoculum size was 0.5%, the temperature was 30℃and the speed was 200r/min, and the culture was carried out for 12 hours.
TABLE 3 Table 3
And (3) taking bacterial liquid, detecting OD600 and ph, and screening out an optimal nitrogen source according to bacterial concentration detection.
The nitrogen source screening results are shown in table 4.
TABLE 4 Table 4
(5) And (5) screening inorganic salts.
(1) And (5) seed culture.
NA medium: 5g of peptone, 3g of beef extract, 5g of NaCl, 1000ml of distilled water and pH7.0. 20 mu L of strain is inoculated into 30ml of liquid culture medium, and cultured for 12 hours at 30 ℃ and 200r/min. Bacterial liquid is taken, OD600 and pH are detected.
(2) And (5) shaking and fermenting.
Initial enzyme production medium: glucose 2.5g, peptone 2g, K 2 HPO 4 ：2g，MgSO 4 ·7H 2 O:0.5g, 1000ml of distilled water, pH7.0. Inoculating the seed bacterial liquid into the initial enzyme production culture medium, and culturing for 12h at 30 ℃ and 200r/min with the inoculum size of 0.5%.
(3) And (5) screening inorganic salts.
3 kinds of culture mediums were prepared in accordance with Table 5 by changing the inorganic salts of the initial enzyme-producing culture medium, and the inoculated amount was 0.5%, at 30℃and 200r/min, and cultured for 12 hours.
TABLE 5
| Peptone | K 2 HPO 4 | MgSO 4 ·7H 2 O | Glucose | FeSO4 | |
| ① | 5g | 2g | 0.05％ | ||
| ② | 5g | 2g | 0.1％ | 2.5g | |
| ③ | 5g | 2g | 0.05％ | 2.5g | 0.016％ |
The test results are shown in Table 6.
TABLE 6
Example 3
Microbial agent deodorization test:
(1) And (5) ammonia removal test.
The liquid culture adopts NA culture medium: 0.5% peptone, 0.3% beef extract, 0.5% sodium chloride, distilled water, and pH was adjusted to 7.0. OD value and pH value were detected, and the culture conditions were 30℃and 200r/min.
The formula of the liquid fermentation medium of the seed tank comprises the following steps: 15g of corn flour, 5g of glucose, 5g of yeast extract, 21g of bean cake powder, 4g of fish meal and CaCO 3 9g,(NH4) 2 SO 4 1.5g,K 2 HPO 4 0.5g，MgSO 4 ·7H 2 O0.1 g, naC10.1g, 1000ml of water, pH 7.5 and inoculum size 0.5%.
Test group: adding 0.25% seed bacterial liquid (namely 1g of bacterial liquid obtained by culturing bacterial strains by adopting a liquid fermentation culture medium of a seed tank), adding 0.5g of glucose, 150ml of water, aerating for 0.3:1/min, adding water to 800ml after 5 hours, and continuing fermentation, wherein the control group: taking 5 new FS pellets, adding 800ml of purified water, and performing fermentation test. After the fermentation, the reference of the fermentation broth for ammonia removal ability was as follows: the results of the detection of the Nahner reagent spectrophotometry for measuring the ammonia in the environment air and the exhaust gas of HJ533-2009 are shown in Table 7.
TABLE 7
| Name of the name | Ammonia concentration before spraying | Spray quantity | Ammonia concentration after spraying | Effect mg/m 3 /g |
| Test group | 95.78 | 2.01g | 13.85 | 40.76 |
| Control group | 95.92 | 2.31g | 20.50 | 32.65 |
(2) Aldehyde removal test.
Laboratory liquid culture uses NA medium: 0.5% peptone, 0.3% beef extract, 0.5% sodium chloride, distilled water, and pH was adjusted to 7.0. OD value and pH value were detected, and the culture conditions were 30℃and 200r/min.
A liquid fermentation culture medium of a seed tank, which comprises the following formula: 15g of corn flour, 4g of glucose, 21g of bean cake powder, 4g of fish meal and CaCO 3 9g,(NH4) 2 SO 4 1.5g,K 2 HPO 4 0.5g,MgSO 4 ·7H 2 O0.1 g, naC10.1g, 1000ml of water, and pH 7.5.
Test group: adding 0.25% bacterial liquid (namely 1g of bacterial liquid obtained by culturing bacterial strains by adopting a liquid fermentation culture medium of a seed tank), adding 0.5g of glucose, 150ml of water, aerating for 0.3:1/min, adding water to 800ml after 5 hours, and continuing fermentation, wherein the control group: taking 5 new FS pellets, adding 800ml of purified water, and performing fermentation test. After the fermentation, the fermentation broth was subjected to the acetylacetone method for measuring the purification effect of an indoor air purification product of Q/BT 2761-2006, GB/T15516-1995, and the results are shown in Table 8.
TABLE 8
| Name of the name | Aldehyde concentration mg/m before spraying 3 | Spray amount/g | Aldehyde concentration mg/m after spraying 3 | 1g removal effect mg/m 3 |
| Test group | 114.41 | 2.17g | 18.94 | 44.00 |
| Control group | 114.67 | 2.13g | 39.14 | 35.42 |
Experiments show that: the Burkholderia cepacia fermentation liquor contains a large amount of deodorizing bacteria, has obvious effects on water purification and odor removal, and has positive significance in the aspects of sewage purification and air purification.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Sequence listing
<110> Hebei Anfu Seisakusho biological Environment technology development Co., ltd
<120> Burkholderia cepacia with deodorizing function and application thereof
<130> 2021.10.21
<141> 2021-11-12
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 405
<212> DNA
<213> Burkholderia cepacia (Burkholderia cepacia)
<400> 1
tggggaatct tgcgcaatgg gcgaaagcct gacgcagcca tgccgcgtga atgatgaagg 60
tcttaggatt gtaaaattct ttcaccgggg acgataatga cggtacccgg agaagaagcc 120
ccggctaact tcgtgccagc agccgtggta atacgaaggg ggctagcgtt gctcggaatt 180
actgggcgta aagggcgcgt aggcggacag ttaagtcggg ggtgaaagcc cggggctcaa 240
cctcggaatt gccttcgata ctggctgtct tgagtacggg agaggtgtgt gggactccga 300
gtgtagaggt gaaattcgta gatattcgga agaacaccag tggcgaaggc gacacactgg 360
cccgttactg acgctgaggc gcgaaagcgt ggggagcaaa cagga 405
Claims (10)
1. The Burkholderia cepacia with the deodorizing function is characterized in that the Burkholderia cepacia is preserved in China general microbiological culture Collection center (CGMCC) No.22231 at the date of 23/04/2021, and is named as Burkholderia cepacia @ in taxonomyBurkholderia cepacia）。
2. The method of claim 1, wherein the seed medium used in the fermentation is NA medium: 0.5% peptone, 0.3% beef extract, 0.5% sodium chloride, distilled water, and pH was adjusted to 7.0.
3. The method of fermentation of burkholderia cepacia according to claim 2, wherein the liquid fermentation medium used in the fermentation is: 15g of corn flour, 4g of glucose, 21g of bean cake powder, 4g of fish meal and CaCO 3 9g, (NH 4 ) 2 SO 4 1.5g, K 2 HPO 4 0.5g，MgSO 4 •7H 2 O0.1 g, naC 1.1 g, 1000ml of water, and pH 7.5.
4. The method of fermentation of burkholderia cepacia according to claim 3, wherein the inoculum size in the fermentation medium is 0.5%.
5. A microbial agent comprising the Burkholderia cepacia according to claim 1.
6. Fermentation broth obtainable by a fermentation process according to any of claims 2-4.
7. Use of the burkholderia cepacia according to claim 1 or the microbial agent according to claim 5 for removing ammonia from sewage.
8. Use of the burkholderia cepacia according to claim 1 or the microbial agent according to claim 5 for removing formaldehyde from sewage.
9. Use of the fermentation process of burkholderia cepacia according to any one of claims 2 to 4 for ammonia removal in sewage.
10. Use of the fermentation process of burkholderia cepacia according to any one of claims 2 to 4 for formaldehyde removal in sewage.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726395A (en) * | 2012-07-05 | 2012-10-17 | 浙江大学 | Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex |
| CN103396971A (en) * | 2013-08-22 | 2013-11-20 | 牛赡光 | Burkholderia cepacia and application thereof |
| KR101489865B1 (en) * | 2013-08-29 | 2015-02-16 | 경북대학교 산학협력단 | Novel strain Burkholderia cepacia JBK9 having antifungial activity against phytopathogenic fungi, its culture, and composition containg them for controlling phytopathogenic fungi |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB0814830D0 (en) * | 2008-08-13 | 2008-09-17 | Univ Cardiff | Antimicrobial agent and method for the production thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726395A (en) * | 2012-07-05 | 2012-10-17 | 浙江大学 | Application of glutaraldehyde cross-linked chitosan for inhibiting growth of burkholderia cepacia complex |
| CN103396971A (en) * | 2013-08-22 | 2013-11-20 | 牛赡光 | Burkholderia cepacia and application thereof |
| KR101489865B1 (en) * | 2013-08-29 | 2015-02-16 | 경북대학교 산학협력단 | Novel strain Burkholderia cepacia JBK9 having antifungial activity against phytopathogenic fungi, its culture, and composition containg them for controlling phytopathogenic fungi |
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