CN105969698A - Composite microbial inoculant for water purification - Google Patents
Composite microbial inoculant for water purification Download PDFInfo
- Publication number
- CN105969698A CN105969698A CN201610546895.8A CN201610546895A CN105969698A CN 105969698 A CN105969698 A CN 105969698A CN 201610546895 A CN201610546895 A CN 201610546895A CN 105969698 A CN105969698 A CN 105969698A
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- China
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- microbial inoculum
- purification
- water
- water body
- bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
Abstract
The invention discloses a composite microbial inoculant for water purification, belonging to the field of microbial preparations. The composite microbial inoculant is composed of photosynthetic bacteria, lactobacillus, nitrobacteria, Bacillus, actinomycete and Bifidobacterium. After being directly added into a water body, the microbial solution can specifically remove or convert ammonia nitrogen, nitrite nitrogen or nitrate nitrogen under different dissolved oxygen conditions, control the inorganic nitrogen components which are harmless to the cultured organisms in the water body environment, quickly restore the ecological environment of the water body and establish a favorable water body environment.
Description
Technical field
The present invention relates to microbial preparation field, be specifically related to a kind of complex microbial inoculum for purification of water quality.
Background technology
Drinking water pollution is mostly derived from water intaking, controlling the water circulation, transmission & distribution water, during water storage etc., due to sanitary sewage, industrial wastewater, the unrest discharge of domestic refuse (rubbish) or industrial solid castoff (waste residue), ignore sanitary of water sources protection, Water warfare and sterilization are the most thorough, the a variety of causes such as the administration of health difference of transmission & distribution water and water storage link, pollute thing and enter in water, make the characteristic of water quality physicochemical property and biotic population, composition changes, cause water quality deterioration, it is detrimental to health, if people has drunk contaminant water, acute and chronic poisoning can be caused, canceration, infectious disease and some other unusual disease, the sense organ that the water polluted causes deteriorates, can bring inconvenience to the life of people, emotion is adversely affected.
Freshwater aquaculture industry is that the mankind are available for the waters cultivated according to the ecological habit of cultivation object and the requirement to water environment condition, utilization, use corresponding breeding facility and technology, is engaged in aquatic economic animal cultivation.In recent years, the culture fishery of China quickly grows, and cultivation scale constantly expands.People are highly efficient and productive in order to pursue, and often put into the substantial amounts of bait rich in nutrient substance such as nitrogens and fertilizer in breeding water body, cause building up of inorganic nitrogen in breeding water body (NH4+-N, NO2--N, NO3--N), affect water ecological setting quality.The quality of breeding water body environment determines the eco-environmental quality of breeding water body, and in breeding water body, three nitrogen (ammonia nitrogen, nitrite nitrogen, nitrate nitrogen) of excessive concentration are huge to aquatic animal and environmental hazard.Current breeding water body denitrogenation modifying agent is broadly divided into chemistry and biological two big classes.Chemical classes modifying agent effect is quick, and effect is directly perceived, and consumption is less, but effective object is single, and the persistent period is short, and often there is ecological insecurity, cures the symptoms, not the disease；Biological species environmental improvement agent is mainly based on microorganism formulations such as bacillus cereus, EM bacterium, and safely and effectively, but onset time is slow, large usage quantity, and effect is unstable by environmental influence.Therefore the nitrate pollution at cultivation water environment control on be badly in need of exploitation and a kind of produce simple, low cost, there is the novel microbial class environmental improvement agent that denitrification effect is not affected by water body environment condition, to meet the needs of industry development.
Microbial bacterial agent has very important effect at aspects such as water environment ecological recovery and water environment purifications.Utilizing microbial bacterial agent to carry out in the reparation of polluted-water, purification process and seldom can produce refuse and discharge pollutants, this water environment being undoubtedly China's pollution deterioration day by day provides a good ecological treatment method, and it has wide market and application prospect.Microbial ecological microbial inoculum has preferable disposal ability to main pollution-producing phosphorus, heavy metal and organic compound in water body, and has the higher ecological value.Therefore microbial bacterial agent is widely used in the sewage purification aspect such as agricultural, industry, the existing microbial ecological preparation processed for water, although obtaining some treatment effects, but generally there is the shortcomings such as microorganism culturing time length, the persistent period is short, microorganism fungus kind is single, the complex micro organism fungicide of the present invention can effectively solve produced problem in above-mentioned sewage purification process.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, the composite bacteria agent capable of a kind of purification of water quality is provided, this microbial inoculum is made up of photosynthetic bacteria, lactic acid bacteria, nitrobacteria, bacillus cereus, actinomycetes, bacillus bifidus, bacterium solution direct plunges into water body, can remove or convert ammonia nitrogen, nitrite nitrogen or nitrate nitrogen under different dissolved oxygen conditions targetedly, control inorganic nitrogen composition harmful to aquaculture organism in water body environment, quickly repair breeding water body ecological environment, build a good water body environment for aquaculture organisms.
A kind of purification of water quality complex microbial inoculum, this composite bacteria agent capable is made up of photosynthetic bacteria, lactic acid bacteria, nitrobacteria, bacillus cereus, actinomycetes, bacillus bifidus, the thalline volume ratio of the most each component is 2～4:0.5-1:1～2:1～3:1～2:0.5～1, the viable bacteria content >=5x10 of described composite bacteria agent capable12cfu/ml。
Preferably, a kind of purification of water quality complex microbial inoculum as above, the bacillus cereus in this microbial inoculum is any one or two kinds of in bacillus firmus, bacillus marinus.
Preferably, a kind of purification of water quality complex microbial inoculum as above, the actinomycetes in this microbial inoculum are streptomyces hygroscopicus.
Preferably, a kind of purification of water quality complex microbial inoculum as above, the photosynthetic bacteria in this microbial inoculum is Crimson rhodospirillum.
Preferably, described a kind of purification of water quality complex microbial inoculum, it is characterised in that: the addition when composite bacteria agent capable uses is the 0.1%-0.5% of sewage quality.
Compared with prior art, the beneficial effects of the present invention is:
1, the microbial inoculum of the present invention is mixed by a certain percentage formed by photosynthetic bacteria, lactic acid bacteria, nitrobacteria, bacillus cereus, actinomycetes, bifid bar so that various microorganisms are complemented each other；Various microorganisms in this microbial inoculum have strong adaptability, and fast breeding becomes dominant microflora.This microbial inoculum is joined in polluted-water, the indexs such as COD in sewage, ammonia nitrogen, total phosphorus, and suppression harmful microbe growth, accelerated decomposition Organic substance in water can be significantly reduced, there is significant purifying water quality.
2, the actinomycetes of the present invention are streptomyces hygroscopicus, and this bacterium is notable to water purification effect, are also to find that streptomyces hygroscopicus has the function of purification of water quality first.
Detailed description of the invention
For the technical characterstic of the present invention program can be clearly described, below in conjunction with specific embodiment, the present invention is illustrated.But protection scope of the present invention is not limited to these embodiments.Within every change without departing substantially from present inventive concept or equivalent replacement are included in protection scope of the present invention.
Embodiment 1
Respectively Crimson rhodospirillum, lactic acid bacteria, nitrobacteria, bacillus marinus, streptomyces hygroscopicus, the strain of bacillus bifidus are inoculated into equipped with activation culture in the shaking flask of volume is shaking flask volume the 30% of culture medium, the strain that activation culture is good is inoculated in fermentation tank and is enlarged cultivating, then will be enlarged by cultivating the bacterium solution obtained to mix according to volume ratio 2:1:2:1:2:0.5, the viable bacteria content of described composite bacteria agent capable is 5.7x1012cfu/ml。
Embodiment 2
Respectively Crimson rhodospirillum, lactic acid bacteria, nitrobacteria, bacillus firmus, bacillus marinus, streptomyces hygroscopicus, the strain of bacillus bifidus are inoculated into equipped with activation culture in the shaking flask of volume is shaking flask volume the 30% of culture medium, the strain that activation culture is good is inoculated in fermentation tank and is enlarged cultivating, then will be enlarged by cultivating the bacterium solution obtained to mix according to volume ratio 3:1:2:3:1.5:1, the mixed volume of bacillus firmus and bacillus marinus is than for 1:1, and the viable bacteria content of described composite bacteria agent capable is 5.7x1012cfu/ml。
Effect assessment: use embodiment 2(minister in ancient times's dragon purification of water quality liquid) prepare the composite bacteria agent capable for purification of water quality and do sewage purification experiment.Take 9 shaking flasks, divide three groups, each shaking flask takes sewage, experimental group 1 adds the composite bacteria agent capable of 0.1%, and experimental group 2 adds the composite bacteria agent capable of 0.05%, and matched group is without anything, binding up with gauze, be placed in constant temperature oscillation illumination box, 24h detects sewage index.Wherein ammonia-nitrogen content uses nessler reagent spectrophotometer (HJ535-2009) to measure, total phosphorus uses ammonium molybdate spectrophotometer method (GB11893-89) to measure, COD detection uses potassium dichromate-Sulfuric-Acid Digestion method (GB11914-89), and experimental result is shown in Table 1.
Claims (5)
1. a purification of water quality complex microbial inoculum, it is characterized in that: this composite bacteria agent capable is made up of photosynthetic bacteria, lactic acid bacteria, nitrobacteria, bacillus cereus, actinomycetes, bacillus bifidus, the thalline volume ratio of the most each component is 2～4:0.5-1:1～2:1～3:1～2:0.5～1, the viable bacteria content of described composite bacteria agent capable
≥ 5x1012cfu/ml。
2. a kind of purification of water quality complex microbial inoculum as claimed in claim 1, it is characterised in that: the bacillus cereus in this microbial inoculum is any one or two kinds of in bacillus firmus, bacillus marinus.
3. a kind of purification of water quality complex microbial inoculum as claimed in claim 1, it is characterised in that: the actinomycetes in this microbial inoculum are streptomyces hygroscopicus.
4. a kind of purification of water quality complex microbial inoculum as claimed in claim 1, it is characterised in that: the photosynthetic bacteria in this microbial inoculum is Crimson rhodospirillum.
5. a kind of purification of water quality complex microbial inoculum as claimed in claim 1, it is characterised in that: the addition when composite bacteria agent capable uses is the 0.1%-0.5% of sewage quality.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610546895.8A CN105969698A (en) | 2016-07-13 | 2016-07-13 | Composite microbial inoculant for water purification |
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| CN201610546895.8A CN105969698A (en) | 2016-07-13 | 2016-07-13 | Composite microbial inoculant for water purification |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754559A (en) * | 2017-02-06 | 2017-05-31 | 青岛农业大学 | A kind of probiotics preparation method for effectively purifying water |
| CN108118015A (en) * | 2018-02-08 | 2018-06-05 | 上海山恒生态科技股份有限公司 | A kind of microbial bacteria agent prescription to purify water |
| CN113955862A (en) * | 2020-07-21 | 2022-01-21 | 天津市富康德环保科技有限公司 | Microbial water purifying agent |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101033450A (en) * | 2007-01-18 | 2007-09-12 | 赵志龙 | Method of producing composite and highly effective microorganism preparation for waste water treatment |
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN102580132A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
| CN103130335A (en) * | 2011-11-27 | 2013-06-05 | 西安瑞捷生物科技有限公司 | Germ composite polluted water treatment agent |
-
2016
- 2016-07-13 CN CN201610546895.8A patent/CN105969698A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101033450A (en) * | 2007-01-18 | 2007-09-12 | 赵志龙 | Method of producing composite and highly effective microorganism preparation for waste water treatment |
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN103130335A (en) * | 2011-11-27 | 2013-06-05 | 西安瑞捷生物科技有限公司 | Germ composite polluted water treatment agent |
| CN102578156A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof |
| CN102580132A (en) * | 2012-01-31 | 2012-07-18 | 玉林市生命宝生物技术有限公司 | Household harmful microorganism elimination and deodorization solution and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754559A (en) * | 2017-02-06 | 2017-05-31 | 青岛农业大学 | A kind of probiotics preparation method for effectively purifying water |
| CN108118015A (en) * | 2018-02-08 | 2018-06-05 | 上海山恒生态科技股份有限公司 | A kind of microbial bacteria agent prescription to purify water |
| CN113955862A (en) * | 2020-07-21 | 2022-01-21 | 天津市富康德环保科技有限公司 | Microbial water purifying agent |
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Application publication date: 20160928 |
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