CN105132342A - GS microbial inoculum for disposing life wastewater and application of GS microbial inoculum - Google Patents
GS microbial inoculum for disposing life wastewater and application of GS microbial inoculum Download PDFInfo
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- CN105132342A CN105132342A CN201510667449.8A CN201510667449A CN105132342A CN 105132342 A CN105132342 A CN 105132342A CN 201510667449 A CN201510667449 A CN 201510667449A CN 105132342 A CN105132342 A CN 105132342A
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- microbial inoculum
- bacillus
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Abstract
The invention discloses GS microbial inoculum for disposing life wastewater and the application of the GS microbial inoculum and belongs to the field of biotechnology. The GS microbial inoculum is constituted by, by weight, 3-9 parts of bacillus, 24-48 parts of lactic acid bacteria and 12-24 parts of nitrobacteria, wherein the bacillus is constituted by methyl trophism bacillus, bacillus subtilis and bacillus amyloliquefaciens, and the lactic acid bacteria are constituted by lactobacillus casei and lactobacillus buchneri. According to the GS microbial inoculum for disposing the life wastewater and the application of the GS microbial inoculum, through identification and safety analysis on bacterial strains, reasonable compatibility is conducted on the bacillus, the lactic acid bacteria and the nitrobacteria, and the GS microbial inoculum for disposing the life wastewater is obtained. After the life wastewater is disposed through the GS microbial inoculum, the removal rate of COD and the removal rate of NH<4>-N reach over 90%.
Description
Technical field
The invention belongs to biological technical field, be specifically related to one way of life sewage disposal GS microbial inoculum and application thereof.
Background technology
Water is the zoic necessary material of institute on the earth, closely bound up with human lives.But along with society, economic developing rapidly, urbanization process is accelerated, a large amount of industrial sewages and sanitary sewage all can be had every day to produce, and water pollution problems has become the focus that people pay close attention to, and treatment rate of domestic sewage has become an important symbol of the whether civilized and civilization degree in area.Sanitary sewage mainly comprises these non-toxic organic compound of carbohydrate, starch, oleosin and urea, mainly causes the eutrophic pollution in river course, lake etc.In recent years, the seven large water systems such as the Changjiang river, the Yellow River, the Zhujiang River, Song Hua River, Huaihe River, Haihe River, the Liaohe River were all subject to pollution in various degree, and national eutrophic lake accounts for more than 50%, and water pollution situation is increasingly severe.
The administering method of sewage mainly contains Physical, chemical method and biological process.Physical (being mainly the precipitator method) process generally can only except the BOD of about 25-30%
5, colloid and deliquescent organism cannot be removed.Chemical method utilizes the organic polymer of synthetic to flocculate, and dosage is large, and easily causes secondary pollution.In Biochemical method technology, microorganism remediation is occupied an leading position, it is the function utilizing microbial decomposition oxidation of organic compounds, create a kind of controllable environment, microorganism raised growth is bred, improve a kind of method of wastewater treatment that it decomposes oxidation efficiency, comprise the pollutent degradation under nature and manual control condition or innoxious process.In recent years, domestic and international experts and scholars carry out the research of microbial treatment sewage one after another, are intended to develop microbial strains fast and effectively, for the improvement of water body environment pollution.Institute of Micro-biology of Chinese science research institute (Liu Zhipei etc., 2007) have studied the application of a kind of South Pole micrococci in low-temperature wastewater treatment, at 10-15 DEG C, effectively can remove the starch in sewage, albumen, fat and BOD, COD etc., embody the using value in low-temperature sewage.Become all Kai sun science and technology (Deng is quiet, 2014) to disclose and utilize low-cost food fermentation microorganism (milk-acid bacteria, Bacillus natto and yeast) and mud self microorganism, the research of sewage quality of making the life better, but effects of purification quality does not have data report.Harbin Institute of Technology (Yankee first etc., 2013) propose pseudomonas, genus bacillus, bacterium nitrobacter, Nitrosomonas and bacillus megaterium as sewage composite fungus agent, strong to adaptive capacity to environment, be applicable to the sewage disposal of northern area microbial augmentation.Shandong Hua Ya Environmental Protection Technology Co., Ltd (Mu Jun equality, 2013) sanitary sewage disposal microbial inoculum is disclosed, comprise polyacrylamide, polymine, silicon-dioxide and complex microorganism composition, wherein microorganism comprises Pseudomonas aeruginosa, Bacillus cereus, Candida lipolytica, nitrobacteria, Paracoccus denitrificans, subtilis.Report display Mu Jun equality microbes closes chemical method scrubbing, be more than 98%, but in microbial inoculum formula, Pseudomonas aeruginosa, Bacillus cereus is pathogenic bacterium, there is certain risk to water suspension, BOD clearance, COD clearance.Separately there is the application of cavity Nocardia bacteria bio-carrier in sanitary sewage disposal adapting to northern low temperature environment, adapt to halophilic decontamination bacterium reports such as process such as waste water after saliferous processing waste water or seawater utilization that brine waste pollutes.
The microorganism type being applied to sewage disposal is at present a lot, comprises for all kinds such as fungi, bacterium, actinomycetes, light and bacteriums.Screening efficient, safe, nontoxic microorganism and be used for water pollutions industry, is the basic demand of social sustainable development.
Summary of the invention
For prior art Problems existing, the object of the invention is to design the technical scheme that one way of life sewage disposal GS microbial inoculum and application thereof are provided.
Described one way of life sewage disposal GS microbial inoculum, it is characterized in that being made up of the composition of following weight part: genus bacillus 3 ~ 9 parts, milk-acid bacteria 24 ~ 48 parts and nitrobacteria 12 ~ 24 parts, described genus bacillus is made up of methylotrophic genus bacillus, subtilis and bacillus amyloliquefaciens, and described milk-acid bacteria Cheesecake Bacterium lacticum and class Lactobacillus buchneri are formed.
Described one way of life sewage disposal GS microbial inoculum, is characterized in that being made up of the composition of following weight part: genus bacillus 6 ~ 9 parts, milk-acid bacteria 24 ~ 36 parts and nitrobacteria 18 ~ 24 parts.
Described one way of life sewage disposal GS microbial inoculum, it is characterized in that described genus bacillus is made up of by weight 1:1:1 mixing methylotrophic genus bacillus, subtilis and bacillus amyloliquefaciens, described milk-acid bacteria Cheesecake Bacterium lacticum and class Lactobacillus buchneri are formed by weight 1:1 mixing.
The application of described GS microbial inoculum in process sanitary sewage.
Described application, it is characterized in that by sanitary sewage disposal GS microbial inoculum by 1 ‰ inoculum size make an addition in sanitary sewage, intermittent aeration, COD, NH after 3 days
4-N clearance all reaches more than 90%.
The present invention, passing through qualification and the safety analysis of bacterial strain, carries out reasonable compatibility to genus bacillus, milk-acid bacteria and nitrobacteria and obtains one way of life sewage disposal GS microbial inoculum.By this GS microbial inoculum to sanitary sewage disposal, COD, NH
4-N clearance all reaches more than 90%.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.The number related in the present invention is weight part.
1 experimental technique
The cultivation of 1.1 bacterium and preparation
Three kinds of genus bacillus, two kinds of bacterial classifications such as milk-acid bacteria, a kind of nitrobacteria are inoculated in liquid nutrient medium respectively, liquid culture based formulas as follows listed by, sterilizing 20min at equal 121 DEG C.
The bacillus culture mediums such as methylotrophic genus bacillus, subtilis, bacillus amyloliquefaciens: wheat bran 2%, Semen Maydis powder 1%, (NH
4)
2sO
40.2%, MgSO
40.05%, KH
2pO
40.3%, prepare with water.
The lactic acid bacteria culturing medium such as lactobacterium casei and class Lactobacillus buchneri: starch 1%, glucose 0.5%, MgSO
40.2%, K
2hPO
40.3%, KH
2pO
40.4%, yeast powder 0.05%, prepares with water.
Nitrobacteria substratum: (NH
4)
2sO
40.2%, NaNO
30.1%, MgSO
40.05%, NaCO
30.1%, FeSO
40.04%, K
2hPO
40.05%, prepare with water.
Different strains is inoculated in liquid nutrient medium by 1% respectively, and 30-36h cultivated by 37 DEG C of shaking tables, and shaking speed is 120r/min, makes bacterial strain be in growth late log phase, collect bacterium liquid, centrifugal 10 minutes of 5000r/min, abandons supernatant, get precipitation, add 20% sterile glycerol, mix rear freeze-drying.Wherein nitrobacteria culture temperature is 40 DEG C, and other operations are with other bacterium.
1.2 combination matching methods
According to respective growth characteristics and the functional performance of methylotrophic genus bacillus, subtilis, bacillus amyloliquefaciens, lactobacterium casei and class Lactobacillus buchneri, nitrobacteria, as rapid in bacillus category growth, milk-acid bacteria and nitrobacteria grow slower; And for example nitrobacteria deammoniation nitrogen ability is strong, the generic bacterium nutritional needs feature such as relatively, and methylotrophic genus bacillus, subtilis and bacillus amyloliquefaciens are classified as a class, are called bacillus category, the weight ratio of three is 1:1:1; Lactobacterium casei and class Lactobacillus buchneri are classified as a class, are called lactic acid bacteria class, both weight ratios are 1:1; Nitrobacteria is classified as separately a class.The different concns arranging three class bacterium is to carry out mixture, and get different compositions and make an addition in self-control sanitary sewage with 1 ‰ inoculum sizes, intermittent aeration, processes under normal temperature, within 3 days, measures chemical oxygen demand COD and the ammonia nitrogen (NH of sanitary sewage after compositions-treated afterwards
4 +-N), investigate clean effect.Experiment proportioning is to design orthogonal L9(
3 4) carry out, often group 5L sanitary sewage Air Exposure.Combination microbial inoculum called after GS microbial inoculum.
Table 1GS microbial inoculum proportioning test L9 (3
4) level of factor table
1.3 self-control sanitary sewages
After family expenses changing food waste (by washing rice, wash dish, wash the combined sewages such as fish, anchovy sauce and fish body, gravy, various vegetables mixed preparing) is mixed defibrination, placed that to adjust COD after within several days, giving out a foul smell be about 500mg/L and get final product, surveying original COD is 582mg/L, ammonia nitrogen NH
4 +-N is 228.3mg/L.
1.4 scrubbing deodorization functions measuring methods
COD assay method: potassium dichromate process, adopts multi-parameter water quality determinator (model: 5B-3B, producer: Lanzhou Lian Hua Environmental Protection Technology Co., Ltd) to measure.
Ammonia nitrogen determination: Berthelot spectrophotometry.Principle is: the basic solution of red mercury iodide and potassiumiodide and ammonia react generate light red brown colloidizing Jie thing, and this color is tool strong absorption in wider wavelength 410-425nm.
2 experimental results:
Test according to the combination of table 1 experimental factor level, experimental result is as following table 2.
Table 2L9(3
4) orthogonal design result
Table 3 analysis of variance table
F1-0.05(2,2)=19.00
According to the characteristic of bacterial strain, with COD clearance for index, bacillus category, lactic acid bacteria class and nitrobacteria 3 factors are utilized to be L9(3
4) orthogonal test.Experimental result is in table 2.Orthogonal experiments intuitive analysis table is extracted from table 2, affecting each factor primary-slave relation of COD clearance is: nitrobacteria > bacillus category > lactic acid bacteria class, show that the optimum strain of sanitary sewage disposal is combined as C3A2B2, i.e. nitrobacteria 24 parts, bacillus category 6 parts, lactic acid bacteria class 36 parts.
Above range analysis is direct result, in order to understand the conspicuous level of experimental error and factor, has follow-uply carried out variance analysis.Viewed from table 3 analysis of variance table, the impact of nitrobacteria on sanitary sewage COD clearance is conspicuous level, bacillus category and lactic acid bacteria class not remarkable on the impact of COD clearance.In conjunction with direct result, selection optimal processing parameter is C3A2B2, i.e. nitrobacteria 24 parts, bacillus category 6 parts, and lactic acid bacteria class 36 parts has carried out proving test on theoretical optimised process basis, and under this combination, process sanitary sewage 3 days, COD clearance is 94%.
Table 4L9(3
4) orthogonal design result
Table 5 analysis of variance table
F1-0.05(2,2)=19.00
According to the characteristic of bacterial strain, with NH
4 +-N clearance is index, utilizes bacillus category, lactic acid bacteria class and nitrobacteria 3 factors to be L9(3
4) orthogonal test.Experimental result is in table 4.Extract orthogonal experiments intuitive analysis table from table 2, affect NH
4 +the each factor primary-slave relation of-N clearance is: nitrobacteria > bacillus category > lactic acid bacteria class, show that the optimum strain of sanitary sewage disposal is combined as C3A3B1, i.e. nitrobacteria 24 parts, bacillus category 9 parts, lactic acid bacteria class 24 parts.
Above range analysis is direct result, in order to understand the conspicuous level of experimental error and factor, has follow-uply carried out variance analysis.Viewed from table 5 variance analysis, nitrobacteria and bacillus category are to sanitary sewage NH
4 +the impact of-N clearance is conspicuous level, and lactic acid bacteria class is to NH
4 +the impact of-N clearance is not remarkable.In conjunction with direct result, selection optimal processing parameter is C3A3B1, i.e. nitrobacteria 24 parts, bacillus category 9 parts, and lactic acid bacteria class 24 parts has carried out proving test on theoretical optimised process basis, under this combination, process sanitary sewage 3 days, NH
4 +-N clearance reaches 97%.
Embodiment 1:
According to genus bacillus, the substratum of milk-acid bacteria and nitrobacteria three class bacterium prepares substratum respectively, and at 121 DEG C sterilizing 20min, respectively corresponding inoculation of medium methylotrophic genus bacillus after cooling, subtilis, bacillus amyloliquefaciens, lactobacterium casei, class Lactobacillus buchneri and nitrobacteria, by postvaccinal liquid nutrient medium except nitrobacteria culture temperature is 40 DEG C, other bacterium cultivate 30-36h in 37 DEG C of shaking tables, shaking speed is 120r/min, bacterial strain is made to be in growth late log phase, collect bacterium liquid, centrifugal 10 minutes of 5000r/min, abandon supernatant, get precipitation, add 20% sterile glycerol, mix rear freeze-drying.
Using methylotrophic genus bacillus, subtilis, bacillus amyloliquefaciens three in the mixing of 1:1:1 ratio as bacillus category, lactobacterium casei mixes as lactic acid bacteria class in 1:1 ratio with class Lactobacillus buchneri.Get nitrobacteria 24 parts, bacillus category 9 parts, lactic acid bacteria class 24 parts mixes, making an addition to 5L with 1 ‰ inoculum sizes makes by oneself in sanitary sewage (COD:582mg/L, NH4+-N:228.3mg/L), intermittent aeration, process under normal temperature, within 3 days, measure chemical oxygen demand COD and the ammonia nitrogen (NH4+-N) of sanitary sewage afterwards, be respectively 56.4mg/L and 5.25mg/L, corresponding COD and ammonia nitrogen removal frank are respectively 90% and 97%.
Embodiment 2:
According to genus bacillus, the substratum of milk-acid bacteria and nitrobacteria three class bacterium prepares substratum respectively, and at 121 DEG C sterilizing 20min, respectively corresponding inoculation of medium methylotrophic genus bacillus after cooling, subtilis, bacillus amyloliquefaciens, lactobacterium casei, class Lactobacillus buchneri and nitrobacteria, by postvaccinal liquid nutrient medium except nitrobacteria culture temperature is 40 DEG C, other bacterium cultivate 30-36h in 37 DEG C of shaking tables, shaking speed is 120r/min, bacterial strain is made to be in growth late log phase, collect bacterium liquid, centrifugal 10 minutes of 5000r/min, abandon supernatant, get precipitation, add 20% sterile glycerol, mix rear freeze-drying.
Using methylotrophic genus bacillus, subtilis, bacillus amyloliquefaciens three in the mixing of 1:1:1 ratio as bacillus category, lactobacterium casei mixes as lactic acid bacteria class in 1:1 ratio with class Lactobacillus buchneri.Get nitrobacteria 24 parts, bacillus category 6 parts, lactic acid bacteria class 36 parts mixes, making an addition to 5L with 1 ‰ inoculum sizes makes by oneself in sanitary sewage (COD:582mg/L, NH4+-N:228.3mg/L), intermittent aeration, process under normal temperature, within 3 days, measure chemical oxygen demand COD and the ammonia nitrogen (NH4+-N) of sanitary sewage afterwards, be respectively 30.7mg/L and 14.25mg/L, corresponding COD and ammonia nitrogen removal frank are respectively 94% and 93%.
Claims (5)
1. one way of life sewage disposal GS microbial inoculum, it is characterized in that being made up of the composition of following weight part: genus bacillus 3 ~ 9 parts, milk-acid bacteria 24 ~ 48 parts and nitrobacteria 12 ~ 24 parts, described genus bacillus is made up of methylotrophic genus bacillus, subtilis and bacillus amyloliquefaciens, and described milk-acid bacteria Cheesecake Bacterium lacticum and class Lactobacillus buchneri are formed.
2. one way of life sewage disposal GS microbial inoculum as claimed in claim 1, is characterized in that being made up of the composition of following weight part: genus bacillus 6 ~ 9 parts, milk-acid bacteria 24 ~ 36 parts and nitrobacteria 18 ~ 24 parts.
3. one way of life sewage disposal GS microbial inoculum as claimed in claim 1 or 2, it is characterized in that described genus bacillus is made up of by weight 1:1:1 mixing methylotrophic genus bacillus, subtilis and bacillus amyloliquefaciens, described milk-acid bacteria Cheesecake Bacterium lacticum and class Lactobacillus buchneri are formed by weight 1:1 mixing.
4. the application of GS microbial inoculum as claimed in claim 1 in process sanitary sewage.
5. apply as claimed in claim 4, it is characterized in that by sanitary sewage disposal GS microbial inoculum by 1 ‰ inoculum size make an addition in sanitary sewage, intermittent aeration, COD, NH after 3 days
4-N clearance all reaches more than 90%.
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| CN108238681A (en) * | 2017-12-22 | 2018-07-03 | 江苏世邦生物工程科技有限公司 | Composite biological agent for low-temperature wastewater treatment and its preparation method and application |
| CN108793603A (en) * | 2018-06-25 | 2018-11-13 | 成都市市政工程（集团）有限责任公司 | Municipal domestic sewage processing method |
| CN110282754A (en) * | 2019-07-09 | 2019-09-27 | 浙江大学常州工业技术研究院 | A kind of breeding wastewater nutritional complexing agent |
| CN110438041A (en) * | 2019-07-22 | 2019-11-12 | 常德职业技术学院 | A kind of Novel compound microbial agent for Treatment of Sludge |
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| CN105524860A (en) * | 2015-12-25 | 2016-04-27 | 杨泽科 | Culture method of fungicide for water purification |
| CN107265612A (en) * | 2017-06-27 | 2017-10-20 | 兰溪市普润斯水产养殖技术有限公司 | Aerobic biochemical water purification agent |
| CN108238681A (en) * | 2017-12-22 | 2018-07-03 | 江苏世邦生物工程科技有限公司 | Composite biological agent for low-temperature wastewater treatment and its preparation method and application |
| CN108793603A (en) * | 2018-06-25 | 2018-11-13 | 成都市市政工程（集团）有限责任公司 | Municipal domestic sewage processing method |
| CN110282754A (en) * | 2019-07-09 | 2019-09-27 | 浙江大学常州工业技术研究院 | A kind of breeding wastewater nutritional complexing agent |
| CN110438041A (en) * | 2019-07-22 | 2019-11-12 | 常德职业技术学院 | A kind of Novel compound microbial agent for Treatment of Sludge |
| CN110468081A (en) * | 2019-09-17 | 2019-11-19 | 湖南艾布鲁环保科技股份有限公司 | Composite bacteria agent and its preparation method and application |
| CN116286436A (en) * | 2022-08-02 | 2023-06-23 | 岳阳渔美康生物科技有限公司 | Lactobacillus buchneri-like, microecological preparation and preparation method thereof |
| CN116286436B (en) * | 2022-08-02 | 2023-11-17 | 岳阳渔美康生物科技有限公司 | Lactobacillus buchneri-like, microecological preparation and preparation method thereof |
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