CN113816503A - Composite microbial agent for domestic sewage microbial treatment - Google Patents

Composite microbial agent for domestic sewage microbial treatment Download PDF

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Publication number
CN113816503A
CN113816503A CN202111208280.1A CN202111208280A CN113816503A CN 113816503 A CN113816503 A CN 113816503A CN 202111208280 A CN202111208280 A CN 202111208280A CN 113816503 A CN113816503 A CN 113816503A
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China
Prior art keywords
parts
domestic sewage
bacteria
microbial inoculum
culture medium
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CN202111208280.1A
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Chinese (zh)
Inventor
李喆
周婧
马路辉
王瑞
张逸飞
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Nanjing Polytechnic Institute
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Nanjing Polytechnic Institute
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Priority to CN202111208280.1A priority Critical patent/CN113816503A/en
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/348Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention discloses a composite microbial inoculum for treating domestic sewage microorganisms, which comprises mixed strains and a culture medium and is characterized in that: the mixed strain comprises flocculant producing bacteria, domestic sewage nutrient component degrading bacteria and aerobic denitrifying bacteria C, wherein the flocculant producing bacteria comprise A1 and A2, the domestic sewage nutrient component degrading bacteria comprise B1, B2, B3, B4, B5 and B6, the mixed strain consists of the following raw material bacteria in parts by weight, A1: 5-10 parts of A2: 5-10 parts of B1: 5-12 parts, B2: 3-10 parts, B3: 10-20 parts, B4: 6-10 parts of B5: 10-15 parts, B6: 4-8 parts of C: 3-5 parts. The flocculant of the invention reduces suspended matters and colloids in domestic sewage, increases the removal rate of COD, and can significantly improve the removal rates of COD, BOD, TOC, ammonia nitrogen, total phosphorus, chromaticity, turbidity and refractory substances, and the effluent is colorless, foamless, clear and transparent.

Description

Composite microbial agent for domestic sewage microbial treatment
Technical Field
The invention relates to the technical field of domestic sewage treatment, in particular to a compound microbial inoculum for domestic sewage microbial treatment.
Background
Domestic sewage contains a large amount of organic matters, suspended solids, nutrient substances and pathogens, and easily causes eutrophication of water bodies and endangers human health. Along with the development of social economy and rapid increase of population quantity in China, the discharge amount of domestic sewage is increased sharply, and the percentage of the total amount of the sewage discharge is increased year by year. Domestic sewage treatment has become the key point of water pollution treatment in China. In recent years, biological technology is widely applied to environmental pollution treatment, wherein biological treatment is a main method for treating sewage, and the biological treatment can realize the advantages of large treatment capacity, low cost, no secondary pollution and the like. The microbial inoculum for sewage treatment mainly considers the degradation performance of strains and the flocculation of microorganisms.
Disclosure of Invention
The invention aims to provide a composite microbial inoculum for treating domestic sewage microorganisms, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a domestic sewage microbiological treatment composite bacterial agent, includes mixed bacterial and culture medium, its characterized in that: the mixed strain comprises flocculant producing bacteria, domestic sewage nutrient component degrading bacteria and aerobic denitrifying bacteria C, wherein the flocculant producing bacteria comprise A1 and A2, the domestic sewage nutrient component degrading bacteria comprise B1, B2, B3, B4, B5 and B6, the mixed strain consists of the following raw material bacteria in parts by weight, A1: 5-10 parts of A2: 5-10 parts of B1: 5-12 parts, B2: 3-10 parts, B3: 10-20 parts, B4: 6-10 parts of B5: 10-15 parts, B6: 4-8 parts of C: 3-5 parts.
Preferably, the culture medium is a simulated domestic sewage culture medium, and the culture conditions are that the initial pH value is 6, the culture temperature is 35 ℃, and the rotating speed of a shaking table is 80 r/min.
Preferably, the effective period of the mixed strain is 30 days.
Preferably, the weight ratio of the mixed strain to the culture medium is 1: 5.
Preferably, the A1, the A2, the B5 and the B6 are bacilli, the B1 and the B2 are mucor, and the B3 and the B4 are streptomyces.
Compared with the prior art, the invention has the beneficial effects that:
the flocculant of the invention reduces suspended matters and colloids in domestic sewage, increases the removal rate of COD, and can significantly improve the removal rates of COD, BOD, TOC, ammonia nitrogen, total phosphorus, chromaticity, turbidity and refractory substances, and the effluent is colorless, foamless, clear and transparent.
Drawings
FIG. 1 is a comparative example of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the composite microbial inoculum for treating the domestic sewage microorganisms comprises a mixed strain and a culture medium, wherein the mixed strain consists of the following raw material bacteria in parts by weight, A1: 6 parts, A2: 9 parts, B1: 8 parts, B2: 5 parts, B3: 14 parts, B4: 8 parts, B5: 13 parts, B6: 4 parts and C: 3 parts, culture medium: 350 parts of (A).
The preparation method of the composite microbial inoculum for treating the domestic sewage microorganisms comprises the following steps: mixing the mixed strain and the culture medium according to a preset weight ratio, uniformly stirring by using a shaking table at a rotating speed of 80r/min, standing for 3 hours, and then keeping the temperature at 35 ℃ to obtain the domestic sewage treatment mixed microbial inoculum.
Example 2:
the composite microbial inoculum for treating the domestic sewage microorganisms comprises a mixed strain and a culture medium, wherein the mixed strain consists of the following raw material bacteria in parts by weight, A1: 7 parts, A2: 10 parts, B1: 6 parts, B2: 8 parts, B3: 16 parts, B4: 6 parts, B5: 11 parts, B6: 7 parts and C: 4 parts, medium: 375 parts of (A).
The preparation method of the composite microbial inoculum for treating the domestic sewage microorganisms comprises the following steps: mixing the mixed strain and the culture medium according to a preset weight ratio, uniformly stirring by using a shaking table at a rotating speed of 80r/min, standing for 3 hours, and then keeping the temperature at 35 ℃ to obtain the domestic sewage treatment mixed microbial inoculum.
Example 3:
the composite microbial inoculum for treating the domestic sewage microorganisms comprises a mixed strain and a culture medium, wherein the mixed strain consists of the following raw material bacteria in parts by weight, A1: 8 parts, A2: 5 parts, B1: 7 parts, B2: 6 parts, B3: 11 parts, B4: 7 parts, B5: 12 parts, B6: 6 parts and C: 5 parts, culture medium: 335 parts.
The preparation method of the composite microbial inoculum for treating the domestic sewage microorganisms comprises the following steps: mixing the mixed strain and the culture medium according to a preset weight ratio, uniformly stirring by using a shaking table at a rotating speed of 80r/min, standing for 3 hours, and then keeping the temperature at 35 ℃ to obtain the domestic sewage treatment mixed microbial inoculum.
And (3) effect testing:
the usage amount of the composite microbial inoculum in sewage is 10g/m3The test result is shown in figure 1, and the test comparison shows that the effect of adding the composite microbial inoculum on the removal rate of COD, BOD, TOC, ammonia nitrogen, total phosphorus, chromaticity, turbidity and refractory substances is more obvious than that of samples which are not added.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides a domestic sewage microbiological treatment composite bacterial agent, includes mixed bacterial and culture medium, its characterized in that: the mixed strain comprises flocculant producing bacteria, domestic sewage nutrient component degrading bacteria and aerobic denitrifying bacteria C, wherein the flocculant producing bacteria comprise A1 and A2, the domestic sewage nutrient component degrading bacteria comprise B1, B2, B3, B4, B5 and B6, the mixed strain consists of the following raw material bacteria in parts by weight, A1: 5-10 parts of A2: 5-10 parts of B1: 5-12 parts, B2: 3-10 parts, B3: 10-20 parts, B4: 6-10 parts of B5: 10-15 parts, B6: 4-8 parts of C: 3-5 parts.
2. The composite microbial inoculum for treating domestic sewage microorganisms according to claim 1, which is characterized in that: the culture medium is a simulated domestic sewage culture medium, and the culture conditions are that the initial pH value is 6, the culture temperature is 35 ℃, and the rotating speed of a shaking table is 80 r/min.
3. The composite microbial inoculum for treating domestic sewage microorganisms according to claim 1, which is characterized in that: the effective period of the mixed strain is 30 days.
4. The composite microbial inoculum for treating domestic sewage microorganisms according to claim 1, which is characterized in that: the weight ratio of the mixed strain to the culture medium is 1: 5.
5. The composite microbial inoculum for treating domestic sewage microorganisms according to claim 1, which is characterized in that: the A1, the A2, the B5 and the B6 are bacilli, the B1 and the B2 are mucor, and the B3 and the B4 streptomyces are streptomyces.
CN202111208280.1A 2021-10-18 2021-10-18 Composite microbial agent for domestic sewage microbial treatment Pending CN113816503A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110373344A (en) * 2019-04-29 2019-10-25 广西科学院 Carp streptomycete and its application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10313853A (en) * 1997-03-19 1998-12-02 Yuji Mae Microorganism-containing semifluid composition, microbial agent and usage thereof
CN101037655A (en) * 2006-11-29 2007-09-19 金明记 High-effective microorganism complex agent and method of using the same for treating city sewage
CN108624541A (en) * 2018-06-28 2018-10-09 绍兴市海清环保科技有限公司 A kind of denitrification mix bacterium agent for textile industry Industrial Waste Water Treatments
CN113151010A (en) * 2021-05-07 2021-07-23 马东兵 Microbial compound bacterium preparation and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10313853A (en) * 1997-03-19 1998-12-02 Yuji Mae Microorganism-containing semifluid composition, microbial agent and usage thereof
CN101037655A (en) * 2006-11-29 2007-09-19 金明记 High-effective microorganism complex agent and method of using the same for treating city sewage
CN108624541A (en) * 2018-06-28 2018-10-09 绍兴市海清环保科技有限公司 A kind of denitrification mix bacterium agent for textile industry Industrial Waste Water Treatments
CN113151010A (en) * 2021-05-07 2021-07-23 马东兵 Microbial compound bacterium preparation and preparation method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
徐志毅: "《环境保护技术和设备》", 31 December 1999, 上海交通大学出版社 *
沈德中: "《环境和资源微生物学》", 31 August 2003, 中国环境科学出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110373344A (en) * 2019-04-29 2019-10-25 广西科学院 Carp streptomycete and its application
CN110373344B (en) * 2019-04-29 2022-07-29 广西科学院 Streptomyces carpio and application thereof

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Application publication date: 20211221