JPH10313853A - Microorganism-containing semifluid composition, microbial agent and usage thereof - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject safe and readily handleable composition by dispersing a microbial raw material containing bacteria, molds, yeasts, actinomyces, etc., in a medium composed of an organic compound which is a liquid at normal temperatures or a viscosity imparting substance added thereto. SOLUTION: This microorganism-containing semifluid composition contains a microorganism dispersed in a medium and is capable of manifesting the semifluidity at normal temperatures. The composition is obtained by dispersing a liquid and/or a solid microbial raw material containing one or more microorganisms such as bacteria, molds, yeasts and actinomyces in a medium composed of an organic compound such as a polyhydric alcohol, a polyether or a nonionic surfactant which is a liquid at 15 deg.C or a medium composed of the organic compound and a viscosity imparting agent such as colloidal silica and/or 12-hydroxystearic acid. The resultant composition useful for decomposing and deodorizing organic pollutants and malodorous substances in domestic wastewater, decomposing and deodorizing animal excreta, decomposing, vanishing and deodorizing garbages, decomposing and deodorizing, etc., organic pollutants and malodorous substances sticking to surfaces of various materials.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism-containing semi-fluid composition, a microbial agent and a method of using the same, and more particularly, to a housewife who is effective in purifying sewage and deodorizing odorous substances. However, the present invention relates to a microorganism-containing composition, a microbial agent, and a method of using them which can be easily handled and exhibit semi-liquid properties such as cream and gel at normal temperature.

[0002]

2. Description of the Related Art It is well known that microorganisms such as protozoa and bacteria play a large role in purification treatment of sewage by activated sludge. Further, attempts to purify the polluted environment by actively utilizing microorganisms such as bacteria have recently become very active. P
Examples of purification of soil contaminated with organic halogen compounds such as CB and trichlorethylene, and bacterial treatment (decomposition treatment) of oil spills such as crude oil are on the news paper. As microorganisms such as bacteria used for these environmental purifications, not only useful microorganisms found from the natural world,
Microbes artificially created by high-tech techniques such as genetic modification have also been studied, and are now at the stage of practical use. The utilization of such microorganisms for environmental purification is not limited to industrial use, and recently, garbage eliminating machines using natural microorganisms have begun to spread to ordinary households. Heretofore, as a microbial agent used in such applications, a powder type and an aqueous solution type have been known.

[0003]

The microbial agent in powder form is
Although the number of viable bacteria can be increased, the effect is excellent in terms of effect expression, but there is a drawback that a part of the bacteria easily disperses into the atmosphere during handling. The aqueous solution type microbial agent can be easily handled, but has a drawback that it is difficult to obtain a product having a large number of viable bacteria and that it is easily spilled. An object of the present invention is to provide a microorganism-containing composition, a microbial agent, and a method for using the same, which can be safely and easily handled by not only specialists but also housewives at ordinary homes without scattering and spilling.

[0004]

Means for Solving the Problems As a result of intensive studies on the above-mentioned problems, the present inventors have found that the above objects can be achieved by combining a microorganism with a specific medium, and have reached the present invention. That is, the present invention relates to a composition which exhibits semi-fluidity at room temperature, in which microorganisms are dispersed in a medium, and comprises bacteria (A1), mold (A2), yeast (A)
3) a liquid and / or solid microbial raw material (A) containing one or more microorganisms selected from actinomycetes (A4)
A microorganism-containing semi-fluid composition dispersed in a medium comprising an organic compound (B) which is liquid at 5 ° C. or a medium comprising (B) and a viscosity-imparting substance (C); A microbial agent in which a substance is sealed in a flexible tube; and a method in which the above-mentioned microbial-containing semifluid composition or microbial agent is used for any of the following applications (1) to (4). (1) Decomposition and deodorization of organic pollutants and odorous substances contained in domestic wastewater. (2) Decomposition and deodorization of human and animal excrement. (3) Decomposition, disappearance and deodorization of garbage. (4) Decomposition and deodorization of organic pollutants and odorous substances adhering to the surface of glass, metal, wood, paper, plastic, and leather.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The microorganisms that can be used in the present invention include, for example, the following bacteria (A1), molds (A2), yeasts (A3) and actinomycetes (A4) used in the field of food industry and the like. Useful microorganisms are mentioned. 1. Bacteria (A1) (1) Nitrosomonas genus (Nitrosomonas) Nitrosomonas europae
a) etc. (2) Nitrobacter spp. Nitrobacter agilis and the like. (3) Thiobacillus genus (Thiobacillus) Thiobacillus thiooxydans (Thiobacillus thiox)
idans). (4) Pseudomonas fluorescens (Pseudomonas fluo)
rescens), Pseudomonas ovalis
alis), Pseudomonas milden barge (Pseudomonas)
mildenbergii) et al. (5) Acetobacter sp. Acetobacter aceti, Acetobacter suboxydans
dans), Acetobacter xylinum
linum) et al. (6) Azotobacter genus Azotobacter chroococcu
m) etc. (7) Rhizobium genus (Rhizobium) Rhizobium leguminosaru
m), Rhizobium trifolii
etc. (8) Aerobacterium (Aerobacter) Aerobacter aerogenes
s) etc. (9) Enterococcus lacti (Enterococcus lacti)
s), Enterococcus cremoris (Enterococcus cr)
emoris), Enterococcus faecalis (Enterococc)
us faecalis), Enterococcus faecium (Enter)
ococcus feacium). (10) Genus Leuconostoc (Leuconostoc)
nteroides) and the like. (11) Lactobacillus bulgaric (Lactobacillus bulgaric)
us), Lactobacillus acidophilus (Lactobacillu)
s acidophilus, Lactobacillus bifidus (Lacto)
lactobacillus bifidus, Lactobacillus delbruekii, Lactobacillus plantarum, Lactobacillus brevis, and the like. (12) Bifidobacterium thermophilum (Bifi
dobacterium thermophilum), bifidobacterium
Pseudolongum (Bifidobacterium pseudolongum)
etc. (13) Bacillus Bacillus subtilis (Bacillus subtilis), Bacillus natto (Bacillus natto), Bacillus megaterium (Bacillus megaterium), Bacillus cereus (B)
acillus cereus), Bacillus polymixer (Bacillus p
olymyxa), Bacillus coagul
ans), Bacillus lichen
iformis). (14) Clostridium butyricu (Clostridium butyricu)
m), Clostridium acetobutylicum
um acetobutyricum), Clostridium saccharoacetobutyricu
m), Clostridium d
issolvans) etc. 2. Mold (A2) (1) Mucor Genus (Mucor pusillus), Mucor rouxii (Mucor rouxii) and the like. (2) Rhizopus Rhizopus nigricans, Rhizopus oryzae, Rhizopus delemar, Rhizopus javanicus, and the like. (3) Phycomyces genus (Phycomyces) Phycomyces nitens,
Phycomyces b
lakesleeanus) and the like. (4) Eremothecium ashbyii (Eremothecium)
etc. (5) Monascus genus (Monascus) Monascus purple (Monascus purpureus), Monascus anka (Monascus Anka) and the like. (7) Neurospora genus (Neurospora) Neurospora sitophila
etc. (8) Claviceps genus (Claviceps) Claviceps purpurea and the like. (9) Genus Aspergillus (Aspergillus) Aspergillus oryzae, Aspergillus tamarii, Aspergillus niger, Aspergillus glaucus, Aspergillus glaucus, and Aspergillus glascus (10) Penicillium genus (Penicillium) Penicillium roquefort
i), Penicillium camembert (Penicillium camem)
berti) et al. (11) Botrytis genus (Botrytis) Botrytis cinera (Botrytis cinerea) and the like. (12) Trichoderma genus (Trichoderma viride) and the like. 3. Yeast (A3) (1) Saccharomyces cerevisiae (Saccharomyces cerevisia)
siae), Saccharomyces Carlsbergensis (Sa
ccharomyces carlsbergensis), Saccharomyces rouxii, Saccharomyces
Bayanus (Saccharomyces bayanus) and the like. (2) Lipomyces Lipomyces starkey and the like. (3) Candida genus (Candida) Candida tropicalis (Candida tropicalis), Candida lipolytica (Candida lipolytica) and the like. (4) Rhodotorula genus (Rhodotorula glutinis)
etc. 4. Actinomycetes (A4) (1) Streptomyces genus (Streptomyces) Streptomyces griseus (Streptomyces grise)
us), Streptomyces aureofaciens (St
reptomyces aureofaciens) and the like.

[0006] Among the bacteria (A1), bacteria of the genus Pseudomonas, Enterococcus, Lactobacillus, Bifidobacterium, Bacillus, and Clostridium are preferred because of their wide range of use and ease of handling. , Bacillus and Clostridium bacteria are more preferred for the same reason, and the spore-forming bacteria Bacillus and Clostridium bacteria are most preferred in that they have excellent heat resistance and can be stored for a long period of time. Among the molds (A2), molds of the genus Mucor, Rhizopus, and Aspergillus are preferable, and among the yeasts (A3), yeasts of the genus Saccharomyces are preferable.

The microorganisms exemplified above are, for example, the fifth edition of the JCM microorganism strain catalog (December 1992, published by RIKEN), LIST OF CULTURES 1992 MICROOGANISMS, NINT.
Inoculation of one or more strains described in H EDITION [May 1992, Fermentation Research Institute] into a medium containing carbon sources, nitrogen sources, inorganic salts, amino acids and vitamins as nutrient sources. And a known culture method. Examples of the carbon source include sugars such as glucose, sucrose, fructose, mannose and xylose; carbohydrates such as starch; vegetable oils such as soybean oil and corn oil; animal oils such as lard; and agricultural products such as bran, wheat, corn and the like. Is mentioned. As the nitrogen source, urea; peptone, meat extract,
Examples include proteins such as casein and yeast extract, and marine products such as fish meal. Examples of the inorganic salts include ammonium phosphate, ammonium chloride, ammonium sulfate, and ammonium nitrate.

As a culturing method, any of a liquid culturing method and a solid culturing method can be applied. It is desirable to select aerobic or anaerobic conditions depending on the nature of the microorganism used. The pH during culturing is preferably from 3 to 9, and more preferably from 4 to 8. The culturing temperature is usually 15 to 60 ° C, preferably 20 to 40 ° C. Culture time is usually 10 hours to 7 hours.
About a day. Although it varies depending on the type of microorganism, enzymes such as protease, amylase, lactase, lipase, hemicellulase, and cellulase are often produced by microorganisms during culture, and play a large role in the function of the composition of the present invention.

In the present invention, a culture containing enzymes and live microorganisms obtained after the culture can be diluted about 2 to 100 times with water and used as a liquid (aqueous solution) microorganism raw material (A). The obtained culture can be dried and powdered as it is to obtain a solid microorganism raw material (A),
Alternatively, adsorbed substances (carriers) such as sawdust, zeolite, perlite, balm curite, celite, acid clay, calcium carbonate, calcium silicate and the like are mixed, dried, powdered or granulated, and solid microbial raw material (A) It can also be. The amount of the adsorbing substance (carrier) used is 99% by mass or less, preferably 10 to 80% by mass, more preferably 30 to 60% by mass in the microorganism raw material (A).

Examples of the organic compound (B) which is liquid at 15 ° C. include a polyhydric alcohol (B1) having 2 to 22 carbon atoms and a molecular weight of 200 to 200 obtained by adding an alkylene oxide (2 to 4 carbon atoms) to an active hydrogen-containing compound. About 30,000 polyethers (B2), surfactants (B3), and other compounds (B4) are exemplified. Examples of (B1) include ethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol, glycerin, diglycerin and the like. (B2)
For example, polyethylene glycol (molecular weight 200 to 600)
Degree), polypropylene glycol, propylene oxide adduct of glycerin, propylene oxide adduct of sorbitol, ethylene oxide and propylene oxide adduct of sucrose, and propylene oxide adduct of bisphenol A. Examples of (B3) include nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and the like. Examples of (B4) include oily compounds such as liquid paraffin, soybean oil, castor oil, and silicone oil, and plasticizers such as dioctyl phthalate and dioctyl adipate. These compounds can be used alone or in combination of two or more. Among (B1) to (B4), (B1), (B2) and (B3) are preferable, and (B1)
In 1), ethylene glycol, propylene glycol, and glycerin are preferable in terms of inexpensiveness, and in (B2), polyethylene glycol (molecular weight of about 200 to 600) and propylene glycol (molecular weight of 200 to 4,
000) and a propylene oxide adduct of glycerin (molecular weight of about 300 to 6,000) are preferred because of their low viscosity and easy handling. Among (B3), nonionic surfactants such as polyhydric alcohol type and polyethylene glycol type are preferable because they have little effect on microorganisms. Among the above, water-soluble ones are preferable because they are easy to use for applications such as treatment of sewage such as domestic wastewater.

When the fluidity of a system in which the microbial raw material (A) is dispersed at 15 ° C. in a liquid medium of the organic compound (B) alone is large, the viscosity-imparting substance (C) can be used together with (B). . Examples of the viscosity-imparting substance (C) include a thixotropic property-imparting substance (d) that increases the viscosity of the system by thixotropy, and a filling substance (e) that increases the viscosity of the system by a filling effect. Examples of (d) include benzylidene sorbitol compounds, amino acid compounds, oil coagulants (d1) such as 12-hydroxystearic acid, and colloidal silica (d2). Colloidal silica (ultra-fine anhydrous silica) is a silica having a primary particle diameter of 7 to 40 nm and a specific surface area of 50 to 380 m 2 / g, called fumed silica obtained by a flame hydrolysis method of silicon tetrachloride. A hydrophilic silanol group (Si-O
H) and a hydrophobic siloxane group (Si-O-Si) and a hydrophobic siloxane group (Si-O-Si).
-Si) is present, for example, Aerosil (manufactured by Nippon Aerosil Co., Ltd.) is commercially available. Among (d1), 12-hydroxystearic acid and a benzylidene sorbitol compound are preferable, and
2-hydroxystearic acid is more preferred because it is inexpensive, has a low melting point and is easy to use. (E) has a melting point of 4
Organic compound (e1) or inorganic compound (e2) at 0 ° C. or higher
Is mentioned. (E1) includes, for example, urea, stearyl alcohol, adipic acid, pentaerythritol,
Low molecular weight organic compounds such as sorbitol, sucrose, benzophenone, hydrogenated castor oil, magnesium stearate, and paraffin; and high molecular weight organic compounds such as polyamide, polyethylene, polystyrene, and polymethacrylate polymers. 0.
Particles of 1 mm or less are preferable, and 0.1 to 30 μm
Fine particles having a particle size of about m are more preferable in that the thickening effect is large. (E2) includes silica, alumina, titanium dioxide, carbon black, iron oxide, anhydrous sodium sulfate, barium sulfate, calcium carbonate, celite, clay,
Examples thereof include talc, glass balloons (hollow glass), and shirasu balloons. Particularly, particles having a particle size of 0.1 mm or less are preferable, and particles having a particle size of about 0.1 to 30 μm have a thickening effect. It is more preferable in terms of a large point. The amount of (C) used when the organic compound (B) liquid at 15 ° C. and the viscosity-imparting substance (C) are used in combination as a medium for microorganisms is as follows:
The thixotropic substance (d) alone is 0.5 to 10% by mass, preferably 1 to 5% by mass with respect to (B), and the filler (e) alone is 10 to 10% by mass with respect to (B). 5
00 mass%, preferably 30 to 300 mass%.
If the amount of (d) used is less than 0.5% by mass or the amount of (e) used is less than 10% by mass, the composition of the present invention has too high a fluidity to easily spill during handling, and the use of (d) If the amount exceeds 10% by mass or the amount of (e) used exceeds 500% by mass, the fluidity of the composition of the present invention is too small to make it difficult to handle. The thixotropy-imparting substance (d) and the filling substance (e) can be used in combination.

Microorganism raw material (A) and medium [(B) +
(C)] is 1/99 to 90 /
10, preferably 10/90 to 70/30, more preferably 30/70 to 50/50. If the compounding ratio (weight ratio) is less than 1/99, it takes too much time to exhibit effects such as deodorization, and if it exceeds 90/10, it becomes difficult to disperse the microorganism raw material in the medium. In the present invention, if necessary, vitamins, nutrients such as minerals, enzymes,
Antibiotics, fragrances, coloring agents, antioxidants, UV absorbers,
Chelating agents and the like can be added.

The microorganism-containing semi-fluid composition of the present invention can be prepared by the following method using a mixing apparatus such as "Universal Mixing Stirrer DM Model" manufactured by Dalton and "Planetary Kneader Mixer" manufactured by Ashizawa. Can be manufactured. 15 ℃
The microbial raw material (A) is added to and mixed with the liquid organic compound (B), and if necessary, the viscosity-imparting substance (C) is further added and mixed to produce the semi-fluid microbial composition of the present invention. Alternatively, a semi-fluid medium is first prepared by mixing the liquid organic compound (B) and the viscosity-imparting substance (C) at 15 ° C., and the microorganism material (A) is charged therein, followed by further stirring. To produce the semi-fluid composition of the present invention. The mixing is usually performed at room temperature, and may be performed by heating to about 35 ° C. in some cases. The mixing time is about 10 minutes to 5 hours, and in most cases, about 20 to 60 minutes is sufficient. However, when the oil coagulant (d1) is used, the entire amount of the oil coagulant (d1) is added to the liquid organic compound (B), and the mixture is heated to dissolve the oil coagulant. It is desirable to add the microbial raw material (A) to the medium obtained after cooling and further mix to produce the semi-fluid composition of the present invention. Further, when the microorganism is a spore-forming heat-resistant bacterium such as a bacterium belonging to the genus Bacillus, a temperature of about 60 to 80 ° C. and a temperature of 5 to 300 ° C.
Mixing may be performed for about a minute. By performing such a heat-resistant treatment, bacteria other than the heat-resistant viable bacteria can be killed, and a semi-fluid composition having excellent storage stability and comprising only spores of the heat-resistant bacteria can be obtained.

Viscosity of the thus obtained semi-fluid composition of the present invention in the form of a cream, gel or paste at room temperature at 25 ° C. (measured at 4 revolutions with a rotational viscometer).
Is usually 2,000 to 1,000,000 cP, preferably 5,000 to 700,000 cP. If the viscosity is less than 2,000 cP, the fluidity of the composition of the present invention is too large, and if the viscosity exceeds 1,000,000 cP, it takes too much time to fill the container, which is not only inefficient, but also causes It is difficult to remove the product from the container. 25 ° C
Viscosity ratio at 4 rpm (viscosity at 4 revolutions / viscosity at 20 revolutions)
Is usually in the range of 1.2 to 20, preferably 2 to 1
It is in the range of 0. If the viscosity is the same, the greater the structural viscosity ratio, the stronger the thixotropic property (thixotropic property). Therefore, the composition is easy to handle because the composition can be extruded from the tube with a small force.

The total viable cell count (including the number of spores in the case of spore microorganisms) contained in the microorganism-containing semifluid composition of the present invention is usually 10 5 to 10 11 CFU / g, preferably 10 6 to 10 11 CFU / g.
10 10 CFU / g. When the viable cell count is less than 10 5 CFU / g, the effect of decomposing and deodorizing the contaminated substances is slowed down, and
If it exceeds U / g, the production cost of the composition increases. In addition, the total viable cell count can be determined according to a known culture method, and can also be determined by using a commercially available food plate (culture plate) as a simple method. Here, CFU is a colony forming unit, and 1
It is considered that one colony is formed when one microorganism is cultured. Therefore, it may be considered that the number of microorganisms before culture and the number of colonies after culture are substantially the same.

The water content of the microorganism-containing semi-fluid composition is usually 30% or less, preferably 10% or less, more preferably 5% or less.
% Or less. The lower the water content, the more dormant the microorganism is, so the storage stability of the composition is better.

In the present invention, the microorganism-containing semifluid composition is preferably filled and sealed in a plastic or metal flexible container or cartridge type container (similar to a container for filling a building sealant). In particular, handling is improved by filling and sealing a flexible tube made of a plastic film or a laminated film into a microbial agent.

As described above, the microorganism-containing semifluid composition of the present invention and the microbial agent filled and sealed in a flexible tube can be handled safely and easily. Housewives can easily use deodorization and washing of bathrooms, and removal of dirt and odor substances adhering to the surface of wood and plastic products, as well as toilets and restaurants at railway stations. It can also be used for business use such as purification of grease straps, and for industrial use such as purification and deodorization of industrial wastewater at paper mills and the like.

[0019]

The present invention will be further described below with reference to examples, but the present invention is not limited to these examples. In the following, parts and% indicate parts by mass and% by mass, respectively. First, the composition and the like of the raw materials used in the examples and comparative examples and the performance evaluation method will be described.

1. Composition of Raw Materials, etc. (1) Microbial Raw Materials (A) For bacteria, molds, yeasts, and actinomycetes, a culture medium suitable for each microorganism including a carbon source such as saccharides, a nitrogen source such as peptone, and minerals, and temperature conditions (25 to 37 ° C)
For about 24 to 72 hours, followed by adding calcium carbonate as a carrier and drying by a conventional method to obtain a powdery microorganism raw material. The results are shown in Tables 1 and 2.

[0021]

[Table 1]

[0022]

[Table 2]

(2) Organic compound (B) liquid at 15 ° C. 1) PG: propylene glycol 2) PPG: polypropylene glycol (molecular weight 2000) 3) Polyether 1: polypropylene glycol (molecular weight 2000) and ethylene oxide (hereinafter simply referred to as EO) 4) Polyether 2: Polyether 5 having a molecular weight of 1500 obtained by adding a 1: 1 mixture (mass ratio) of EO and PO (propylene oxide) to n-butanol. ) Surfactant 1: Nonionic surfactant with 6 moles of EO added to octylphenol 6) Surfactant 2: Nonionic surfactant of oleic diester of polyethylene glycol (molecular weight 600) (3) Viscosity-imparting substance (C) 1) 12HSA: 12-hydroxystearin Acid 2) Colloidal silica: Aerosil # 200 manufactured by Nippon Aerosil Co., Ltd. 3) Filling substance 1: Calcium carbonate (4) Additive 1) Fragrance: Fragrance "Rose" (powder) 2) Colorant: Food additive "Blue" No. 1) 3) Antioxidant: 2,6-di-t-butyl-4-methyl-phenol (DBP)

2. Performance Evaluation Method (1) Thixotropy (Thixotropic) Using a rotational viscometer (manufactured by Shibaura System Co., Ltd .: Vismetron VGH viscometer), the viscosity was determined from the ratio of the viscosity at 20 rotations at 25 ° C. to the viscosity at 4 rotations. Structural viscosity ratio = (Viscosity at 4 rotations) / (Viscosity at 20 rotations) (2) Total viable cell count The sample is diluted to an appropriate concentration with sterile physiological saline, and the diluted solution is used to facilitate the use of a culture plate. The total viable cell count (CFU / g) was determined by the method.・ Culture plate: Sanai Bio-Checker TTC manufactured by Sanai Oil Co., Ltd. ・ Culture conditions: 35 ° C. × 72 hrs (3) Ammonia gas concentration measurement It was measured by a suction method using a commercially available detector tube.・ Detector: Detector tube type gas measuring device GV-100S (manufactured by Gastec) ・ Detector tube: Ammonia detector tube No. 3L (Gastec)

Examples 1 to 9 The semi-fluid composition containing microorganisms of the present invention was prepared according to the formulations shown in Tables 3 and 4, and 300 g of the composition was filled in a polypropylene film tube to prepare the microbial agent of the present invention. Obtained.
The performance evaluation results are shown in Tables 3 and 4.

[0026]

[Table 3]

[0027]

[Table 4]

Next, an example of the use of the microorganism-containing semifluid composition (microbial agent) of the present invention in a standard family of four families will be described. Usage Example 1 Household garbage disposer (Sanyo Electric garbage nice SNS)
1 Kg of wood chips were placed in the tank of -K1), and about 1 liter of water was sprinkled on the chips. Next, after about 300 g of the composition (microbial agent) of Example 7 was sprinkled on the chip, the lid was closed, and then the stirring in the treatment tank was started. Two hours after the start of stirring, about 500 g of drained garbage (fresh vegetables, fruits, cooked scraps, and the like having a size of about 3 cm or less) was charged. After that, add about 500g of garbage every day and pay attention to the amount of water in the treatment tank.
When it was drenched, the garbage input was stopped for 2-3 days) and the garbage treatment was continued for 90 days. Odor (fermentation / rot odor) was observed until the 4th day of operation,
After that, the odor was reduced to a level that was not bothersome. Also, assuming that the average water content of the garbage input is 75%,
The extinction rate of garbage was calculated from the mass of the solid collected after the treatment, and was found to be about 90%. Half of the collected solid was left in the soil for about two months to age and used as compost, and the other half was treated as combustible waste. Use Example 2 About 10 g of the composition (microbial agent) of Example 5 is squeezed out immediately after the last person's use into the drain port of a bath washing area used by the whole family every day, and about 1 liter of the remaining hot water in the bath is used. Washes away. This operation was performed at approximately the same time every day for another 6 days. Then, once using about 20 g of the same microbial agent /
The same operation was continued for two months at a weekly pace. The unpleasant odor peculiar to sewage which rises through the drain port and was recognized before the use of the microbial agent decreased sharply from the third day of use and decreased by 10%.
Almost no more after the day. In addition, the moldy and unpleasant odor coming up from the drain of the washing machine that leads to the drain of the bathroom was significantly reduced one month after the start of the test in the bathroom, and almost two months after the start of the test in the bathroom. No side effect was observed. Use Example 3 After washing dinnerware and the like, about 1 composition (microbial agent) of Example 2 was placed in a drainage net trap of a stainless steel kitchen sink.
0 g was added and left, and the same operation was continued for 6 days. Thereafter, the same operation was continued once a week for one month. The unpleasant odor from the drain outlet observed before using the microbial agent of the present invention disappeared from the third day of use,
After one month, the slimy feeling of the wire mesh trap recognized before the start of use was reduced. Also, a drainage ditch (basin: 30 × 30) installed outdoors from around the 10th day of use.
X 30 cm) in the stored water. Use Example 4 About 10 g of the composition (microbial agent) of Example 4 was squeezed out into a hand-washing part of an on-tank flush toilet and was flushed with water. This operation was performed every three days and continued for two months. From the 7th day after the use of the composition of the present invention, the portion of the toilet that is exposed to water becomes less likely to be stained, and the beautiful state of the toilet is tested by cleaning the toilet once a week without using a cleaning agent. Continued until the end. In addition, the unpleasant odor drifting in the toilet room from the tenth day was reduced. Use Example 5 Since the clothes chest immediately after purchase emits an unpleasant odor (which is considered to be derived from the adhesive) in a closed state, about 40 g of the composition (microbial agent) of Example 8 is divided into several parts to be clothed. I attached it to the piece and wiped the entire surface of the inside of the clothes chest. When left undisturbed for one week, the unpleasant odor was reduced, and when the entire surface of the inside of the clothes chest was wiped with a wet cloth and dried naturally, the unpleasant odor almost disappeared.

Next, an example of use of the microorganism-containing semifluid composition (microbial agent) of the present invention for business use will be described. Use Example 6 After washing a male urinal (automatic flushing type) used about 60 times a day on average with a weak alkaline detergent twice a week,
About 10 g of the composition of Example 6 (microbial agent) was sprinkled on the upper part of the urinal dish and washed with water. This operation was continued for three months. In addition, the ammonia concentration in the lower space of the perforated plate was measured immediately before the start of use, 7 days, 20 days, 50 days, 9 days from the start of use.
Measurement was performed using a detector tube for each of the 0 days later. Table 5 shows the results. Seven days after the use of the microorganism-containing composition (microbial agent) of the present invention, the ammonia concentration was reduced to one third, and the ammonia odor was completely eliminated after the 20th day of use.

[0030]

[Table 5]

As shown in Tables 3 and 4, since the semi-fluid composition containing microorganisms (microbial agent) of the present invention has an appropriate fluidity, it can be easily extruded from a flexible tube and is easy to handle. As is clear from Use Examples 1 to 6, the microorganism-containing semi-fluid composition of the present invention (microbial agent)
It can be seen that is excellent in decomposability of organic matter, deodorizing property, clarification of sewage and the like.

[0032]

EFFECTS OF THE INVENTION The microorganism-containing composition (microbial agent) of the present invention which is semi-fluid at normal temperature has an appropriate viscosity and is easy to handle. It can be handled safely and easily. In addition, the composition (microbial agent) of the present invention utilizes the organic substance decomposability inherent in microorganisms, thereby decomposing garbage, deodorizing and purifying domestic wastewater, and deodorizing toilets. The present invention can be applied to antifouling of a toilet bowl, furthermore, removal of an unpleasant odor emitted from the surface of a material such as wood, and the effect can be continued for a long time. Therefore, the microbial-containing semi-fluid composition and the microbial agent of the present invention are not only used in general households as described above, but also used for business such as purification of grease traps in restaurant kitchen wastewater, and furthermore, wastewater treatment and the like. It is also promising for industrial applications.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI C12N 1/16 C12N 1/20 D 1/20 F B09B 3/00 ZABD // (C12N 1/00 C12R 1: 125 1:10 ) (C12N 1/00 C12R 1: 125 1:10 1:12 1:09) (C12N 1/00 C12R 1: 125 1:01 1:39) (C12N 1/00 C12R 1: 125 1:10 1: 01 1: 845) (C12N 1/00 C12R 1: 125 1:10 1:01 1:69 1:85 1: 465) (C12N 1/20 C12R 1: 125) (C12N 1/20 C12R 1:39)

Claims (6)

[Claims] 1. A composition showing a semi-fluidity at room temperature, in which microorganisms are dispersed in a medium, selected from bacteria (A1), molds (A2), yeasts (A3), and actinomycetes (A4). A liquid and / or solid microbial raw material (A) containing one or more microorganisms is prepared from a medium comprising an organic compound (B) which is liquid at 15 ° C., or from (B) and a viscosity-imparting substance (C). Microorganism-containing semi-fluid composition dispersed in a medium. 2. The microorganism-containing semi-fluid composition according to claim 1, wherein the microorganism raw material (A) contains one or more sporangial bacteria. 3. The microorganism-containing half according to claim 1, wherein the organic compound (B) liquid at 15 ° C. is at least one selected from polyhydric alcohols, polyethers, and nonionic surfactants. Flowable composition. 4. The microorganism-containing semifluid composition according to claim 1, wherein the viscosity-imparting substance (C) is colloidal silica and / or 12-hydroxystearic acid. 5. A microbial agent, wherein the microorganism-containing semifluid composition according to claim 1 is sealed in a flexible tube. 6. A method of using the microorganism-containing semi-fluid composition according to any one of claims 1 to 4 or the microorganism agent according to claim 5 for any of the following uses. (1) Decomposition and deodorization of organic pollutants and odorous substances contained in domestic wastewater. (2)
Decomposition and deodorization of human and animal excrement. (3) Decomposition, disappearance and deodorization of garbage. (4) glass, metal, wood, paper,
Decomposition and deodorization of organic pollutants and malodorous substances adhering to the surface of plastic and leather.