JP2010194532A - Treating agent for cleaning wastewater and treating method for cleaning wastewater - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a treating agent for cleaning wastewater and a treating method for cleaning wastewater which can remarkably reduce the load for cleaning a grease trap. <P>SOLUTION: The treating agent for cleaning wastewater is used which includes mixed lactic acid bacteria comprising Lactobacillus fermentum and Enterococcus faecium, and pineapple enzyme. Preferably, the mixed lactic acid bacteria is carried with a hydrous gel, and the pineapple enzyme is formed by mixing with a water-soluble organic binder and further preferably contains Bacillus subtilis. A process (1) of cleaning wastewater by bringing the mixed lactic acid bacteria and the pineapple enzyme into contact with wastewater is included in the method. Further, a process (2) of cleaning wastewater by bringing the wastewater cleaned in the process (1) into contact with Bacillus subtilis is desirably included. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a waste water purification treatment agent and a waste water purification treatment method. More specifically, the present invention relates to a wastewater purification treatment agent suitable for purifying domestic wastewater or kitchen wastewater, and a purification treatment method for these wastewater, and is particularly provided in kitchens in the restaurant industry such as restaurants, restaurants and hotels. The present invention relates to a waste water purification treatment agent that is optimal for purifying waste water flowing into a grease trap and a purification treatment method for these waste water.

  In general, in the restaurant industry such as restaurants, restaurants, hotels, etc., the drainage from these kitchens contains a large amount of oil and fat, and when the oil and fat are cooled, it solidifies and clogs the drainage system (pipe). Since there is a danger, it is obliged to provide a grease trap for removing these oils and fats.

  Such wastewater flowing into the grease trap includes wastes such as fats and oils, leftovers, vegetable scraps, etc., and the wastes become oily substances composed of solid oils and liquid oils. Has become a scum in the pit of the grease strap and generates a rotting odor. For this reason, daily cleaning of the grease wrap is being carried out, but the current situation is that the operation is complicated and very difficult. Specifically, daily cleaning of the grease wrap must be done in a small place after the end of business hours (for example, at midnight), and it is extremely difficult to completely clean the deposits of oil and fat and sludge. is there. On the other hand, if you ask a specialist to clean the grease wrap every day, the cost burden increases, and it is difficult to install a dedicated purification device in a small kitchen, and the cost burden is also high. There is.

  Therefore, in view of such points, conventionally, aerobic bacteria (Patent Document 1), microbial preparations that produce enzymes such as lipases (Patent Document 2), active soil that performs ion exchange, and components that generate negative ions Purification of waste water as described above is also performed using a water purification material containing water (Patent Document 3), ozone gas (Patent Document 4) and the like.

JP-A-10-277573 JP 2003-266062 A JP 2006-26516 A JP 2008-23460 A

  However, even if a conventional waste water purification treatment agent or purification treatment method is applied, the oil and fat content in the waste water flowing into the grease trap cannot be sufficiently decomposed. For this reason, if the grease trap is not frequently cleaned, the oil and fat components become oily turbidity, and scum is generated in the grease strap or a spoiled odor is generated. Therefore, the grease wrap must be cleaned frequently, and this burden (person, cost, etc.) is a problem. That is, an object of the present invention is to provide a wastewater purification treatment agent and a wastewater purification treatment method that can remarkably reduce the burden of cleaning a grease wrap.

The feature of the wastewater purification treatment agent of the present invention is a mixed lactic acid bacterium comprising Lactobacillus fermentum and Enterococcus faecium,
The gist of the invention is that it contains and contains a pineapple enzyme.

  A feature of the method for purifying wastewater of the present invention is a step of purifying wastewater by bringing mixed lactic acid bacteria consisting of Lactobacillus fermentum and Enterococcus faecium into contact with pineapple enzyme and wastewater. The point including (1) is summarized.

  The wastewater purification treatment agent of the present invention has an extremely high ability to purify wastewater. This ability appears particularly in wastewater containing a large amount of fats and oils. Therefore, when the waste water purification treatment agent of the present invention is applied to the grease trap, the grease or the like does not scum in the grease strap or generate a rot odor without cleaning the grease strap. The burden is significantly improved.

  The waste water purification treatment method of the present invention can effectively purify waste water. This ability appears particularly in wastewater containing a large amount of fats and oils. Therefore, when the drainage purification method of the present invention is applied to a grease wrap, the grease or the like does not scum in the grease wrap or generate a decaying odor without cleaning the grease wrap. The burden to do is significantly improved.

  In the conventional technology (wastewater purification treatment agent), the limit of biological oxygen demand (hereinafter referred to as “BOD”) is about 250 ppm, but in the wastewater purification treatment agent of the present invention, it is reduced to 150 ppm or less. can do.

  Moreover, in the conventional waste water purification treatment agent, the limit was set to about 250 ppm of the normal hexane extract substance in the waste water, but in the waste water purification treatment agent of the present invention, it can be reduced to 6 ppm or less.

FIG. 2 is a plan view conceptually depicting a grease trap optimal for applying the waste water purification treatment agent of the present invention as viewed vertically from the ground. FIG. 2 is an end view conceptually drawn by cutting the grease strap shown in FIG. 1 in the vertical direction. It is the side view on which the microorganism group package used in the Example was drawn notionally. It is the side view on which the enzyme package used in the example was drawn notionally. 1 is a side view conceptually depicting a container that can accommodate a wastewater purification treatment agent of the present invention. FIG. 6 is a plan view conceptually depicting the container shown in FIG. 5 as viewed vertically from the ground. FIG. 7 is a side view conceptually depicting a state in which the lid body is removed from the container body with respect to the container shown in FIGS. 5 and 6. In the grease trap applied in the Example, it is sectional drawing drawn conceptually by cut | disconnecting the state which is ventilating the waste_water | drain which flows in in a perpendicular direction.

Lactobacillus fermentum is a plant lactic acid bacterium and has the morphological property of a fungus that it is a koji mold. In addition, as a physiological property of bacteria, it produces gas from glucose, has acid resistance, can survive even under strong acidity (pH 2.0), is resistant to heat, and can survive at 60 ° C for 30 minutes. Is possible.
Lactobacillus fermentum can be easily obtained from the market, for example, from SKY Life Co., Ltd.

Enterococcus faecium is also a plant lactic acid bacterium, and has the morphological property of a bacterium that is a cocci. In addition, the physiological properties of the fungus are that it does not produce gas from glucose, grows under alkaline conditions of pH 9.6 or acidic conditions of pH 4.8, has high bile resistance, is strong at low temperatures, and survives at 4 ° C. Is possible.
Enterococcus faecium can also be easily obtained from the market, for example, from SKY Life Co., Ltd.

  A mixed lactic acid bacterium composed of Lactobacillus fermentum and Enterococcus faecium produces various useful enzymes (for example, amylase, protease, lipase, etc.) in a wide range of temperature and pH, and these useful enzymes greatly contribute to purification of waste water. Contribute.

  Although there is no restriction | limiting in the ratio of each microbe contained in the mixed lactic acid bacteria which consist of a Lactobacillus fermentum and Enterococcus faecium, From a viewpoint of the purification processing capacity of waste water, (the number of bacteria of a Lactobacillus fermentum) / (Enterococcus faecium bacteria) The number) is preferably 0.5 to 1.5, more preferably 0.7 to 1.3, particularly preferably 0.8 to 1.2, and most preferably 0.9 to 1.1.

  As a mixed lactic acid bacterium composed of Lactobacillus fermentum and Enterococcus faecium, powders (SKY Life Co., Ltd.) having the same number of lactic acid bacteria can be used.

The mixed lactic acid bacteria composed of Lactobacillus fermentum and Enterococcus faecium may be used as a dry powder, a liquid dispersion, or a support supported on various carriers. When used as a carrier, the type of carrier is not limited, but includes hydrogels (gels, jellies, etc.), and examples include agar gels. Among these, from the viewpoint of handleability, a liquid dispersion and a support are preferable, a support is more preferable, and a hydrogel is particularly preferable.
In addition, it is preferable to use agar as the hydrous gel because it also serves as a medium for lactic acid bacteria. That is, when agar is used, lactic acid bacteria can grow on an agar gel.

  The pineapple enzyme is an enzyme extracted from pineapple juice, has an excellent ability to degrade proteins, includes various enzymes (such as those listed in paragraph 0009 of Japanese Patent No. 2849018), and directly. Contributes to the purification of wastewater.

  Pineapple enzyme is mainly produced in Southeast Asia, and can be obtained from TUSCOT Co., Ltd. (Thailand), Ho Chi Minh Agricultural Products (Vietnam Socialist Republic), and others.

  For the pineapple enzyme, an extract from pineapple juice may be used as it is, or a concentrated concentrate of the extract or a powder obtained by drying the extract may be used, or a mixture molded with a water-soluble organic binder. It may be a body (such as a granular body). The water-soluble organic binder is not limited as long as it can be dissolved in water and imparts moldability, but starch and the like can be preferably used from the viewpoint of load on waste water and availability. Among these, from the viewpoints of storage stability and handleability, a dry powder and a molded body are preferable, and a molded body is more preferable. In the case of forming a molded body, a shape having a surface area as large as possible is preferable in order to ensure a contact area with drainage, and examples thereof include a granular shape (a maximum diameter of about 5 to 15 mm, preferably about 10 mm).

Mixed lactic acid bacteria (two types of microorganisms) consisting of Lactobacillus fermentum and Enterococcus faecium and pineapple enzyme may be mixed to form an integrated wastewater purification treatment agent, but without mixing these separately It is preferable to use the constructed waste water purification treatment agent.
In the case where the mixed lactic acid bacteria and the pineapple enzyme are separately configured without being mixed, for example, each can be separately packaged with a packaging material to obtain a waste water purification treatment agent. That is, it can be set as the waste water purification treatment agent comprised from the microorganisms package body which packaged mixed lactic acid bacteria (2 types of microorganisms) with the packaging material, and the enzyme package body which packaged the pineapple enzyme with the packaging material.

  When the mixed lactic acid bacteria (two kinds of microorganisms) and the pineapple enzyme are packaged with such a packaging material, handling properties and workability are further improved.

  Various materials can be used as the packaging material, and the material is not particularly limited. For example, a bag made of a composite sheet obtained by laminating a polyethylene sheet and a polyamide sheet is preferable. It can be illustrated. In the case of such a composite sheet, it is preferable to make a bag so that the polyamide sheet comes into contact with the mixed lactic acid bacteria and the pineapple enzyme, and the polyethylene sheet comes into contact with the outside air. When a mixed lactic acid bacterium comes into contact with a polyethylene sheet or polypropylene sheet, the lactic acid produced by the mixed lactic acid bacterium polymerizes to form polylactic acid crystals, which may break the packaging material. Is preferably polyamide.

The number of bacteria contained in the mixed lactic acid bacteria (CFU / g) is preferably 10 ⁵ to 10 ⁸ and more preferably 10 ⁶ to 10 ⁷ based on the weight of the mixed lactic acid bacteria and the pineapple enzyme.
The bacterial count {CFU (Colony forming unit) / g} can be measured according to the BCP-added plate agar medium method.

The content of the pineapple enzyme is preferably 800 to 1200, more preferably 1000 to 1100, based on the weight of the mixed lactic acid bacterium and the pineapple enzyme, as bromelain activity (unit / g-dry).
In addition, bromelain activity is measured according to the food additive official standard (unit / g-dry is a unit per dry weight).

  It is preferable that the wastewater purification treatment agent of the present invention further contains Bacillus subtilis. When Bacillus subtilis is included, it is possible to decompose organic substances that cannot be purified by useful enzymes and pineapple enzymes produced by mixed lactic acid bacteria.

  Bacillus subtilis has the ability to coexist with different kinds of useful microorganisms, is a bacilli with both ends blunted, and is said to withstand tens of minutes at 100 ° C. Moreover, although the optimal temperature is 27-37 degreeC, it can proliferate well at 5-50 degreeC, and the optimal pH is 7-8.5, but it can proliferate also in 4-10, glucose (glucose), maltose (maltose) It produces organic acids, vitamin B group and UGF (unknown growth promoting factor) from sucrose but does not make gas.

Bacillus subtilis is ubiquitous in nature such as hay, milk, soil, miso and air, and can be easily obtained from, for example, commercially available natto.
Bacillus subtilis produces various useful enzymes (for example, amylase, protease, lipase, etc.), and these useful enzymes greatly contribute to purification of waste water.

  As Bacillus subtilis, Bacillus subtilis var. Natto is preferable from the viewpoints of availability and the like, although Bacillus subtilis can be widely used. Bacillus natto can be easily obtained, for example, by the method shown in the examples.

  Bacillus subtilis may be used as a dry powder, may be used as a liquid dispersion, or may be used as a carrier supported on various carriers. When used as a carrier, the type of carrier is not limited, but includes hydrogels (gels, jellies, etc.), and examples include agar gels. Among these, from the viewpoint of handleability, a liquid dispersion and a support are preferable, a support is more preferable, and a hydrogel is particularly preferable.

When the wastewater purification treatment agent of the present invention contains Bacillus subtilis, it may be mixed with a mixed lactic acid bacterium (two types of microorganisms) and / or pineapple enzyme to form an integrated wastewater purification treatment agent, but without mixing with these. It is preferable that the wastewater purification treatment agent is configured separately.
When the Bacillus subtilis, the mixed lactic acid bacterium, and the pineapple enzyme are separately configured without being mixed, for example, similarly to the above, each can be separately wrapped with a packaging material to be a drainage purification treatment agent. When such a packaging material is used to package Bacillus subtilis, mixed lactic acid bacteria (two types of microorganisms), and pineapple enzyme, handling and workability are further improved.

  Various materials can be used as the packaging material, and the material is not particularly limited. For example, a bag made of a composite sheet obtained by laminating a polyethylene sheet and a polyamide sheet (polyamide) Preferably, the sheet is made in contact with a mixed lactic acid bacterium or a pineapple enzyme and the polyethylene sheet is in contact with the outside air.

When the wastewater purification treatment agent of the present invention contains Bacillus subtilis, the Bacillus subtilis content (CFU / g) is preferably 10 ⁵ to 10 ⁸ , more preferably 10 based on the weight of the mixed lactic acid bacterium and pineapple enzyme. ^6, which is a 10 ^7.

The method for purifying wastewater of the present invention will be described.
In the step (1) of purifying the wastewater by bringing the mixed lactic acid bacteria consisting of Lactobacillus fermentum and Enterococcus faecium into contact with the pineapple enzyme and the wastewater, the mixed lactic acid bacteria (two kinds of microorganisms) ) And the pineapple enzyme may be mixed and the waste water purification treatment agent and waste water may be brought into contact with each other, but the waste water purification treatment agent separately constituted without mixing them and the waste water may be brought into contact with each other. Is preferred.

  In the step (1), when contacting the wastewater purification treatment agent separately constituted without mixing the mixed lactic acid bacteria (two kinds of microorganisms) and the pineapple enzyme, and the wastewater, there is no limitation in the order of contacting the wastewater, It is preferable to first contact with mixed lactic acid bacteria (including useful enzymes produced by mixed lactic acid bacteria) and then contact with pineapple enzyme.

  The amount of the mixed lactic acid bacteria (two kinds of microorganisms) and the pineapple enzyme used can be adjusted as appropriate depending on the amount of waste water and the degree of dirt.

  The wastewater purification treatment method of the present invention preferably further includes a step (2) of purifying the wastewater by bringing the wastewater purified in the step (1) into contact with Bacillus subtilis.

  When the method for purifying wastewater according to the present invention includes the step (2), the amount of Bacillus subtilis used can be appropriately adjusted depending on the amount of wastewater and the degree of contamination.

When mixed lactic acid bacteria (two types of microorganisms), pineapple enzyme and, if necessary, Bacillus subtilis are packaged in packaging material, the packaging material may be torn (breaked) and the contents directly put into the waste water .
The mixed lactic acid bacteria (two kinds of microorganisms), pineapple enzyme and, if necessary, Bacillus subtilis may be directly put into the waste water, but after storing in a container, it is preferable to contact the waste water, more preferably the mixed lactic acid bacteria (2 (Species microorganism), pineapple enzyme, and, if necessary, Bacillus subtilis are separately contained in a container for use. By storing in a container, the disappearance of mixed lactic acid bacteria (two types of microorganisms), pineapple enzyme and, if necessary, Bacillus subtilis can be easily confirmed, and maintenance is further facilitated. That is, mixed lactic acid bacteria, pineapple enzymes and, if necessary, Bacillus subtilis are discharged into the wastewater and gradually disappear by contact with the wastewater. Such disappearance reduces the weight of these mixed lactic acid bacteria, and as a result, the mixed lactic acid bacteria may float in the wastewater and be discharged from the wastewater treatment facility. When stored in a container, it is possible to prevent discharge due to such weight reduction.

Examples of the container include a resin net container, a metal net container, and a container having a hole (hole container).
There is no limitation on the shape of the container, and it may be a substantially square bag shape, a substantially circular bag shape, a substantially elliptic bag shape, a substantially columnar shape (substantially cylindrical shape), a substantially quadrangular prism shape, a substantially conical shape, a substantially quadrangular pyramid shape, a substantially spherical shape, or the like. included.
Note that “abbreviated” means a shape that is similar to, a shape that approximates to, a shape that is approximately.

  When using containers, different types of containers can be used in combination. Examples of combinations of different types of containers include a combination in which a resin mesh container is placed in a wire mesh container, a combination in which a resin mesh container is placed in a hole container, and a combination in which a wire mesh container is placed in a hole container.

Examples of the resin of the resin net container include polyamide, polypropylene, polyethylene, and polyurethane.
The mesh (μm) of the resin mesh container and the wire mesh container is preferably about 10 to 30, more preferably about 15 to 25.

  Examples of the hole container include a container in which a hole is formed in the container having the above shape, and a container in which a part of the container having the above shape is replaced with a resin net or a metal net.

Examples of the hole container include a hole container 8 including a substantially cylindrical container body 9 and a lid body 10 that can open and close the opening 12 at the top of the container body 9 (FIGS. 5 to 7). The container body 9 and the lid body 10 constituting such a hole container 8 may be entirely made of synthetic resin or the like. The type of synthetic resin is not critical, but for example, polyvinyl chloride and polyethylene can be used.
A hole 11 as shown in FIGS. 5 and 7 is formed in a substantially central portion of the container body 9. By forming such a hole 11 in the container main body 9, drainage flows into the container main body 9 through the hole 11.
A male screw may be formed in the lower portion 13 of the lid body 10. In this case, a female screw that can be screwed into the male screw is formed on the inner surface side immediately below the opening 12 at the top of the container body 9. The lid 10 can be attached to and detached from the upper portion of the container main body 9 by screwing and unscrewing the male screw and the female screw.
A handle 14 may be formed on the upper portion of the lid 10 by projecting three ribs 14a, 14b, 14c at equiangular intervals as shown in FIGS. 6 to 7 (14a, 14b, 14c Although not shown in the drawing, it means the three ribs shown in FIG.
By gripping the handle 14, an operation for attaching and detaching the lid 10 to and from the upper portion of the container main body 9 can be easily performed.
The hole 11 may be closed with a resin net or a metal net.

  The waste water purification treatment agent and waste water purification treatment method of the present invention can be widely applied to waste water containing biodegradable substances, but is suitable for domestic waste water or kitchen waste water, and particularly, restaurants, restaurants, hotels, etc. It is most suitable for drainage flowing into the grease traps provided in kitchens in the restaurant industry.

  The temperature of the drainage is not limited as long as it is not frozen, but is preferably 0 to 40 ° C, more preferably 4 to 30 ° C. That is, according to the waste water purification treatment agent and the waste water purification treatment method of the present invention, waste water can be purified efficiently even at 30 ° C. or lower, 12 ° C. or lower, and 5 ° C. or lower.

  Although there is no restriction | limiting as pH of a waste_water | drain, 5-7 are preferable, More preferably, it is 5.2-6.1. In order to adjust the pH of the wastewater to a suitable range, a pH adjuster (such as sodium bicarbonate, citric acid, malic acid, acetic acid and sodium salts of these acids) may be used in combination.

The basic structure of a general grease trap for treating wastewater that is optimal for applying the wastewater purification treatment agent and the wastewater purification treatment method of the present invention will be described below.
The grease strap 1 includes a partition plate 2 as shown in FIGS. 1 and 8, and as shown in FIG. 2, air is discharged into the drainage 3 such as domestic wastewater or kitchen wastewater accommodated in the greasetrap 1. Is provided with an air supply pipe 4 for feeding air and a pump 5 serving as a supply source for feeding air. Such a configuration is also the same as that of a general grease strap. The grease strap 1 has various sizes depending on the amount of drainage and the like, for example, one configured as a tank of 1 m in length × 1 m in width and 1.5 m in depth. In FIG. 1, a state where the grease strap 1 is partitioned by the partition plate 2 is schematically illustrated, and an outer frame and the like are not illustrated.

  A blower can be installed in the grease strap 1 as an aeration device. As the blower ability, for example, one that blows 40 liters of air into a 1 m × 1 m × 1.5 m pit per minute is used. However, the low aeration is desirably 5 hours per day, and depending on the activity status, the level of aeration can be adjusted.

  The inside of the grease trap 1 is a breeding environment for all kinds of germs, and there are many anaerobic bacteria, the proliferation of filamentous fungi and actinomycetes is excessive, the dissolved oxygen in the sewage is reduced, and the environment becomes anoxic. If the amount of released air (oxygen amount) is limited, the environment where oils and fats can be oxidatively decomposed is lost, resulting in an oxygen deficient state and a foul odor.

  By installing the wastewater purification treatment agent of the present invention on the grease trap 1 or applying the wastewater treatment method of the present invention, an environment in which such fats and oils can be oxidatively decomposed and an unpleasant odor environment is remarkably produced. Can be improved.

Examples of the present invention will be described below. Unless otherwise specified, “part” means “part by weight” and “%” means “% by weight”.
<Preparation of mixed lactic acid bacteria>
(1) Lactobacillus fermentum pure culture solution 1.5 parts, skim milk (skim milk powder) 4 parts, natural salt 1 part, molasses 1 part, sodium glutamate 0.5 part, starch starch 4 parts, defatted soybean powder 6 parts and water 82 parts were uniformly mixed, dried in a circulating drier (50 ° C., 50 hours), pulverized, and then a seed mesh 1 having a mesh size of 180 μm or less was adjusted using a wire mesh.

(2) Inoculum 2 was obtained in the same manner as in (1) above except that the Lactobacillus fermentum pure culture solution was changed to Enterococcus faecium pure culture solution.

(3) Each of the seed mother bacteria 1 and the seed mother bacteria 2 was uniformly mixed by 2.5 parts to obtain a mixed bacteria (powder).
8 parts agar (Ina Food Industry Co., Ltd.) is mixed with 1000 parts of water and heated up to 93 ° C. while uniformly dissolving, then boiled for 5 to 10 minutes and stirred at low speed (about 25 rotations per minute) While gradually lowering the temperature, the temperature was lowered to 40 ° C. to obtain a gel raw material.
Add 5 parts of the mixed bacteria (inoculum 1 and 2) to this gel stock, stir for about 1 hour, and then add 3 parts of saccharides (glucose, brown molasses) as nutrients for the mixed bacteria and stir. When the temperature was lowered to 25 ° C., a gel-like mixed lactic acid bacterium was obtained. The number of bacteria contained in the gel-like mixed lactic acid bacteria was 1.2 × 10 ⁷ CFU / g.

By storing 1 kg of the gel-like mixed lactic acid bacteria in a packaging material, deaeration, and completely sealing and sealing the open part of the packaging material with a sealing device, a microbial group package (mixed lactic acid bacteria) 6 as shown in FIG. Obtained.
The packaging material is a laminate sheet obtained by laminating a 35 μm-thick polyethylene sheet and a 20 μm-thick polyamide sheet into a bag 30 cm long × 25 cm wide with the polyamide sheet inside.

<Preparation of pineapple enzyme>
Pineapple enzyme purchased from TUSCOT Co., Ltd. (Thailand) (enzyme 70%, starch 30%; bromelain activity 1090 units / g-dry; moisture 11.7%; granular with a diameter of about 10 mm) is used as it is. Used as.
4 kg of pineapple enzyme was packaged with a packaging material to obtain an enzyme package 7 as shown in FIG. The same packaging material as that used for the mixed lactic acid bacteria was used.

<Preparation of Bacillus subtilis>
(1) Soybean natto (Tsurunoko Natto Honpo, Takahashi Food Industry Co., Ltd.) 20g was crushed with a juicer mixer (YAMAZEN juice mixer MJM-T760, Yamazen Co., Ltd.) until it became muddy, and then gauze from the muddy material. After squeezing out the filtrate, 100 ml of this filtrate was uniformly mixed with 1000 ml of pure water, added with 5 g of glucose, 2 g of peptone and 2 g of yeast extract, and cultured at about 40 ° C. for 48 hours. Bacillus subtilis culture solution (natto culture solution) )

(2) 8 parts of agar (Ina Food Industry Co., Ltd.) is mixed with 1000 parts of water and heated up to 93 ° C. while uniformly dissolving, then boiled for 5 to 10 minutes, at low speed (about 25 rotations per minute) While stirring, the temperature was gradually decreased to 40 ° C. while gradually decreasing the temperature to obtain a gel raw material.
After adding 20 parts of Bacillus subtilis culture solution to this gel substance and stirring for about 1 hour, 3 parts of saccharides (glucose, brown molasses), which are nutrient sources for the mixed bacteria, are added and stirred to adjust the temperature. When the temperature was lowered to 25 ° C., gel-like Bacillus subtilis (gel-like Bacillus natto) was obtained. The number of bacteria contained in the gel-like Bacillus subtilis was 10 ⁷ CFU / g.

  1 kg of this gel-like Bacillus subtilis was housed in a packaging material, deaerated, and the open part of the packaging material was completely sealed with a sealer to obtain a microorganism group package (Bacillus subtilis) 6.

<Example 1>
By applying the wastewater purification treatment agent of the present invention to an oil trap (yield amount 10t / day; FIG. 8) installed in a yakitori shop for one month as follows, the wastewater discharged from the oil trap is BOD and normal. The hexane extract was measured and shown in Table 1.
BOD was tested by the method defined in “21. Biochemical Oxygen Consumption (BOD)” and “32.3 Diaphragm Electrode Method” of JIS K0102: 2008. For normal hexane extract, JIS K0102: It was tested by the method defined in “24. Hexane Extracted Substance” and “24.2 Extraction Method” in 2008.

  Break the microbial group package (mixed lactic acid bacteria), take out 1 kg of gel-like mixed lactic acid bacteria, store it in a net made of polyamide 35 cm long x 30 cm wide, and place it between the oil trap partition plate and partition plate It was hung near the partition near the drainage inlet.

  Break the enzyme package, take out 1kg of pineapple enzyme, store it in a net made of polyamide 35cm long x 30cm wide, and close to the drainage outlet between the oil trap partition and partition Hanging around.

  Break the microbial group package (Bacillus subtilis), take out 1kg of gel-like mixed lactic acid bacteria, store it in a net made of polyamide 35cm long x 30cm wide, and between the oil trap partition plate and the drain outlet Hung on.

<Comparative Example 1>
In Example 1, BOD and normal hexane extract substances were measured for waste water discharged from an oil trap immediately before application of the waste water purification treatment agent of the present invention, and are shown in Table 2.

<Example 2>
Except that the microbial group package (Bacillus subtilis) was not used for the oil trap installed in the food supermarket (about 5,000 to 6,000 visitors; wastewater volume 30 t / day; FIG. 8). The waste water purification treatment agent of the present invention was applied for 1 month, and BOD and normal hexane extractable substances were measured for the waste water discharged from the oil trap.

<Comparative example 2>
In Example 2, BOD and normal hexane extract substances were measured for waste water discharged from an oil trap immediately before application of the waste water purification treatment agent of the present invention, and are shown in Table 2.

<Example 3>
In the same manner as in Example 1, the waste water purification treatment agent of the present invention was applied to an oil trap (about 100 people; waste water volume 20 t / day; FIG. 8) installed in a social welfare corporation cafeteria for 1 month. The waste water discharged from the trap was measured for BOD and normal hexane extract and shown in Table 1.

<Comparative Example 3>
In Example 3, BOD and normal hexane extract substances were measured for waste water discharged from an oil trap immediately before application of the waste water purification treatment agent of the present invention, and are shown in Table 2.

<Example 4>
In the same manner as in Example 1, the wastewater purification treatment agent of the present invention was applied to the oil trap (about 1500 people; wastewater volume 20 t / day; FIG. 8) installed in the cafeteria of the university of the Kansai region for one month. When applied, the normal hexane extract was measured for the wastewater discharged from the oil trap and shown in Table 1.

<Comparative example 4>
In Example 4, BOD and normal hexane extract substances were measured for waste water discharged from an oil trap immediately before application of the waste water purification treatment agent of the present invention, and are shown in Table 2.

As is apparent from Tables 1 and 2, when the wastewater purification treatment agent and the wastewater purification treatment method of the present invention were applied, BOD and normal hexane extractable substances could be significantly reduced.
Further, in the comparative example, although the inside of the oil trap was cleaned every day, the smell of rotting was present, but in the examples, the rotting was performed even though the operation of discarding the solid in the solid basket was performed every day. It did not smell.
As described above, when the wastewater purification treatment agent and the wastewater purification treatment method of the present invention are applied, the burden of cleaning the grease trap can be significantly reduced.

DESCRIPTION OF SYMBOLS 1 Grease strap 2 Partition plate 3 Drainage 4 Air supply pipe 5 Pump 6 Microorganism group packaging body 7 Enzyme packaging body 8 Container 9 Container body 10 Lid body 11 Hole 12 Upper part of container body 13 Lower part of lid body 14 Handle

Claims (9)

Mixed lactic acid bacteria consisting of Lactobacillus fermentum and Enterococcus faecium,
A wastewater purification treatment agent comprising a pineapple enzyme. The wastewater purification treatment agent according to claim 1, wherein the mixed lactic acid bacteria are supported on a hydrous gel, and the pineapple enzyme is mixed and molded together with a water-soluble organic binder. The number of bacteria contained in the mixed lactic acid bacteria is 10 ⁵ to 10 ⁸ CFU / g based on the weight of the mixed lactic acid bacteria and the pineapple enzyme, and the bromelain activity of the pineapple enzyme is 800 to 1200 units / based on the weight of the mixed lactic acid bacteria and the pineapple enzyme. The waste water purification treatment agent according to claim 1 or 2, which is g-dry. Furthermore, the waste water purification processing agent in any one of Claims 1-3 containing Bacillus subtilis. The waste water purification treatment agent according to claim 4, wherein the Bacillus subtilis is Bacillus subtilis var. Natto. The waste water purification treatment agent according to claim 4 or 5, wherein Bacillus subtilis is supported on a hydrous gel. The wastewater purification treatment agent according to any one of claims 4 to 6, wherein the number of bacteria contained in Bacillus subtilis is 10 ⁵ to 10 ⁸ CFU / g based on the weight of the mixed lactic acid bacterium and the pineapple enzyme. The method includes a step (1) of purifying wastewater by contacting a mixed lactic acid bacterium composed of Lactobacillus fermentum and Enterococcus faecium with a pineapple enzyme and wastewater. Purification treatment method. The wastewater purification treatment method according to claim 8, further comprising a step (2) of purifying the wastewater by bringing the wastewater purified in the step (1) into contact with Bacillus subtilis.

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