JPH10309311A - Quickly soluble microorganisms contained tablet - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable safe and easy handling without scattering powder in case of handling by molding powdery or granular raw materials composed of microorganisms agents, foaming agents and binders into tablets. SOLUTION: The microorganisms agent is made powdery while containing microorganisms composed of bacteria, molds and yeast more than one kind and further containing enzyme, medium and carrier or the like. As the foaming agent, the agent of a type to discharge carbon dioxide gases through contact with water can be provided. As the binder, a water soluble or water dispersion organic or inorganic binder in solid state at 20 deg.C can be used. Concerning the production of tablet, the required components of microorganisms agent, foaming agent and binder and the arbitrary component of extending agent and additive as needed are mixed in a powder mixing tub such as Nauta mixer, afterwards, coarse grains are removed through the sieve of about 10 to 50 mesh and mixed raw materials are adjusted. Next, molding into tablet is performed by a rotary tablet machine and a tablet for about 3 to 100 g can be provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism-containing tablet, and more particularly to a kitchen or bathroom sink, a toilet deodorant,
The present invention relates to a tablet containing microorganisms, which is effective in deodorizing a septic tank and decomposing and eliminating garbage, and is rapidly soluble in water.

[0002]

2. Description of the Related Art Microbial agents are generally weak to heat and are easily deactivated in the presence of water, but can be stored for a long time under dry conditions. For this reason, powdered microbial agents are more commonly used than microbial agents as liquid microbial agents.For powdered microbial agents, cells isolated from a normal culture solution can be used as they are or as flour or bran. It is manufactured by adding low-temperature aeration drying, vacuum drying or freeze-drying, to which organic matter such as rice bran or the like, or inorganic matter such as diatomaceous earth or calcium carbonate is added.

[0003]

However, the powdered microbial agent produced in this way has a disadvantage that the powder is liable to fly during handling. An object of the present invention is to provide a safe and fast-dissolving microbial-containing tablet that does not flour during handling, and that can be easily handled by persons without specialized knowledge such as housewives in general households as well as specialists. It is in.

[0004]

Means for Solving the Problems As a result of intensive studies on the above problems, the present inventors have found that the above objects can be achieved by tableting a powdery microbial agent together with a foaming agent, and have reached the present invention. did. That is, the present invention is a microorganism-containing tablet obtained by tablet-forming a powdery or granular raw material comprising a microbial agent (A), a foaming agent (B), and a binder (C).

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, a microbial agent (A)
Contains one or more types of microorganisms such as bacteria, molds, yeasts, etc., and further contains enzymes, culture media, carriers and the like, and is in a powder form. The microorganisms in (A) are in a dormant (inactive) state because they contain substantially no water (about 5% by mass or less). Examples of the microorganism that may be contained in the microbial agent (A) include, for example, Bacillus, Lactobacillus, Streptococcus, and Enterococcus.
cus), Enterobacter, Nitrosomonas, Nitr
bacterium, Cellulomonas, Pseudomonas, Agrobacterium (A
grobacterium), Rhizopus, Aspergillus, yeast and the like. These microorganisms can be used in combination of two or more.
When they are used together, they often coexist and cooperatively act (share roles) under the same environment and often effectively act on decomposition of organic substances, malodorous substances, and the like.

Among the above microorganisms, Bacillus (Bacill)
us), Lactobacillus, Streptococcus, Enterococcus, and Nitrosomona
s), Nitrobacter, Rhizopus, Aspergillus, and Saccharomyces microorganisms have a large ability to purify sewage by fermenting and decomposing organic matter such as oil in sewage. And Bacillus, Lactobacillus, Streptococcus, Enterococcus
bac), and more preferably a bacterium belonging to the genus Bacillus, which is a thermostable bacterium.

In the present invention, examples of the species of the above microorganisms include the following. (1) Bacillus (Bacillus) Bacillus subtilis (IFO (Institute for Ferment)
ation Osaka: Fermentation Research Institute, hereinafter the same) 1371
9), Bacillus natto (IFO3013), Bacillus licheniformis (IFO12200), Bacillus coagulans (IFO12583), Bacillus macerans (IFO3490), Bacillus megaterium (IFO1208), Bacillus polymixer (IFO1)
3003). (2) Lactobacillus (Lactobacillus) Lactobacillus casei (IFO12521), Lactobacillus acidophilus (IFO13951), Lactobacillus delbrukki (IFO3202), and the like. (3) Streptococcus genus (Streptococcus) Streptococcus faecalis (IFO3971),
Streptococcus cremoris (IFO3427),
Streptococcus lactis (IFO12007),
Streptococcus salivarius (IFO1395
6), Streptococcus thermophilis (IFO1
3957), Streptococcus faecium (IFO)
12256). (4) Enterococcus Enterococcus faecalis (IFO3971, IF
O3889), Enterococcus feciliam (IFO)
3128, IFO3535) and the like. (5) Enterobacter Enterobacter Aerogenis (IFO12010,
IFO 13534). (6) Genus Nitrosomonas (Nitrosomonas) Nitrosomonas europaea (IFO14298) and the like. (7) Nitrobacter, Nitrobacter agiris (IFO 14297) and the like. (8) Cellulomonas (Cellulomonas) Cellulomonas flavigena (IFO3754), Cellulomonas biazotea (IFO12680), Cellulomonas seracea (IFO3748), Cellulomonas uda (IFO3747), Cellulomonas gerida (IFO3)
748). (9) Pseudomonas (Pseudomonas) Pseudomonas putida (IFO14164), Pseudomonas nitroreducens (IFO12694)
Such. (10) Agrobacterium Agrobacterium tumefaciens (IFO30
58, IFO 12667). (11) Rhizopus Rhizopus oryzae (IFO4706), Rhizopus formosaensis (IFO4732), Rhizopus japonicas (IFO4732), Rhizopus delemar (IFO4746) and the like. (12) Aspergillus (Aspergillus) Aspergillus oryzae (IFO30104), Aspergillus niger (IFO31125), Aspergillus usamii (IFO4388), Aspergillus
Sawyer (IFO4391), Aspergillus Terreus (IFO31217) and the like. (13) Saccharomyces Saccharomyces cerevisiae (IFO10217),
Saccharomyces Carlsbergensis (IFO11
67).

In the present invention, the microbial agent (A) can be produced by a conventionally known method, for example, as follows. That is, an appropriate amount of the above-mentioned microorganisms (one or more), a medium, and a carrier are added to water and mixed, and the mixture is added at a temperature of 25 to 50 ° C.
After culturing for about 72 hrs, the mixture is vacuum-dried by, for example, a spray-drying method and powdered. As the culture medium, agricultural and marine products such as fish meal, oily scum, bran, wort and its waste can be used, and as the carrier, inorganic or organic such as calcium carbonate, magnesium carbonate, calcium silicate, diatomaceous earth, zeolite, sawdust and the like. A system powder can be used. The microbial agent (A) thus obtained contains one or more enzymes such as protease, amylase, lactase, lipase, hemicellulase, and cellulase, which are usually produced by microorganisms during culture. In the present invention, in order to further enhance the functions of the microbial agent (A) such as organic substance decomposability and deodorization, enzymes other than those described above can be added to the microbial agent (A). It can be added later.

The total viable count of microorganisms contained in 1 g of the microbial agent (A) is usually about 10 ⁸ to 10 ^11.
It is preferable that the viable cell count of each of the microorganisms is in the following range from the viewpoint of decomposability of organic matter, deodorization, and the like. That is, (1) Bacillus subtilis: 10 ⁸ to 10 ¹¹ / g (2) Lactobacillus: 10 ⁶ to 10 ¹⁰ / g (3) Streptococcus: 10 ⁶ to 10 ¹⁰ / G (4) Enterococcus: 10 ⁶ to 10 ⁹ / g (5) Enterobacter: 10 ⁶ to 10 ⁹ / g (6) Nitrosomonas: 10 ⁶ to 10 ⁹ / G (7) Nitrobacter: 10 ⁶ to 10 ⁹ / g (8) Cellulomonas: 10 ⁶ to 10 ⁹ / g (9) Pseudomonas: 10 ⁶ to 10 ⁸ / g g (10) Agrobacterium: 10 ⁶ to 10 ⁸ / g (11) Rhizopus: 10 ⁶ to 10 ⁹ / g (12) Aspergillus: 1 ^{0 6 ~10 9 / g (13} ) the genus Saccharomyces ^{(Saccharomyces): 10 6 ~10 8} / g

In the present invention, the amount of the microbial agent (A) is 10% by mass or more, preferably 20 to 60% by mass, more preferably 20 to 50% by mass in the tablet. When the amount of the microbial agent (A) is less than 10% by mass, it takes time for the microorganisms to self-proliferate, and as a result, the onset of effects such as deodorization is delayed.

Examples of the foaming agent (B) include those which release carbon dioxide upon contact with water.
Specific examples thereof include a two-component system of a carbonate (b1) and an acid (b2). Carbonates (b1) and acids (b2)
Is usually 30:70 to 80:20, preferably 40:60 to 70:30. Examples of the carbonate (b1) include carbonates of alkali metals such as sodium carbonate, potassium carbonate, sodium hydrogen carbonate, potassium hydrogen carbonate and sodium sesquicarbonate; carbonates of alkaline earth metals such as magnesium carbonate, calcium carbonate and strontium carbonate Salts. Among the alkali metal carbonates and alkaline earth metal carbonates, alkali metal carbonates are preferred in that they are easily dissolved in water, and among alkali metals, sodium carbonate and sodium hydrogen carbonate are more inexpensive. preferable. Examples of the acids (b2) include inorganic acids and organic acids which are solid at room temperature. The inorganic acids are preferably weakly acidic, and specific examples thereof include boric acid, metasilicic acid, and silicic anhydride. Examples of the organic acid include compounds such as carboxylic acid, sulfonic acid, and sulfamic acid. Among the inorganic acid and the organic acid, the organic acid is more preferable in that a tablet having high strength is easily obtained. Among the organic acids, a weakly acidic carboxylic acid compound is preferable, and among them, citric acid, fumaric acid, maleic acid, succinic acid,
Aliphatic carboxylic acid compounds such as tartaric acid, malic acid, adipic acid and azelaic acid are more preferred because they do not inhibit the activity of microorganisms. The amount of the foaming agent (B) is 1 to 5 in the tablet.
0 mass%, preferably 2 to 30 mass%, more preferably 5 to 20 mass%. When (B) is less than 1% by mass, the dissolution rate of the tablet in water is low, and when it exceeds 50% by mass, product stability such as partial reaction of carbonates and acids with the passage of time, foaming and disintegration of the tablet. Gets worse.

Examples of the binder (C) include organic or inorganic binders which are water-soluble or water-dispersible and are solid at 20 ° C. Specific examples of the organic binder include polyethylene glycol (c1); trihydric or higher polyhydric alcohols such as glycerin, pentaerythritol, sorbitol, and sucrose; and active hydrogen-containing compounds such as ethylenediamine, diethylenepolyamine, and triethanolamine. A polyalkylene oxide adduct (c2) to which ethylene oxide alone or ethylene oxide and propylene oxide are added; polyoxyethylene alkyl (C8 to C22) ether, polyoxyethylene alkyl (C8 to C22) phenol ether,
Pluronic surfactants, tetronic surfactants, nonionic surfactants (c3) such as fatty acid (C8 to C20) glyceride, modified starch (c4) such as dextrin, soluble starch, and hydroxypropyl starch; Cellulose modified products such as hydroxypropylcellulose and hydroxypropylmethylcellulose (c
5) and the like. Among these organic binders,
Polyethylene glycol (c1), modified starch (c
4) is preferable in that a tablet having a large adhesive strength and a high strength is obtained, and polyethylene glycol having a molecular weight of 2,000 to 10,000 is particularly preferable. Examples of the inorganic binder include sodium silicate. The amount of the binder is 1 to 50% by mass in the tablet, preferably 3 to 50% by mass.
20% by mass. If (C) is less than 1% by mass, the tablet is brittle and fragile, and if it exceeds 50% by mass, the dissolution rate of the tablet becomes slow.

In the present invention, an extender (D) can be used if necessary. Examples of the bulking agent (D) include inorganic bulking agents (d1) such as sodium chloride, sodium sulfate, potassium sulfate, sodium nitrate, potassium nitrate, calcium phosphate, aluminum silicate, gypsum, kaolin, and zeolite; lactose, glucose, sucrose, Organic extenders (d2) such as D-sorbitol, D-mannitol, pectin, starch, flour, microcrystalline cellulose and the like. The amount of the bulking agent (D) used is 0 to 88% by mass in the tablet,
Preferably it is 20-70 mass%.

In the present invention, if necessary, additives (E) such as a lubricant, a coloring agent, a fragrance, an antioxidant, an ultraviolet absorber, a chelating agent (metal scavenger) and the like can be used.

The method for producing the microorganism-containing tablet of the present invention is generally as follows. That is, first, (A),
(B), essential components of (C), and if necessary (D),
After mixing the optional components of (E) in a powder mixing tank such as a Nauta mixer, a coarse material is removed by passing through a sieve of about 10 to 50 mesh to prepare a compounding raw material. Next, tableting (compression) molding is performed using this compounded raw material by, for example, a rotary tableting machine to obtain a tablet of about 3 to 100 g.

The microbial-containing tablet thus obtained is about 2
When immersed in 5 ° C. still water, it collapses while releasing gas, and loses and dissolves in lump in about 1 to 10 minutes (if insolubles exist, the insolubles settle or float on the water surface).

The number of viable bacteria in the microbial agent (A) or the microbial-containing tablet (unit: number / g) is determined by appropriately selecting the culture conditions using an ordinary medium such as a standard agar medium (for example, 35 (° C. × 24 to 72 hours) It can be measured according to a known method such as a mixed flat culture method.

[0018]

EXAMPLES The present invention will be further described with reference to examples below, but the present invention is not limited to these examples. In the following, parts and% indicate parts by mass and% by mass, respectively. First,
The compositions of the raw materials used in Examples and Comparative Examples are shown.

<Raw material composition> Microbial agent (A) (1) a-1: about 50% of carrier (calcium carbonate)
Bacillus subtilis (IFO13
719) 8 × 10 ⁹ / g, Bacillus licheniformis (IFO12200) 1 × 10 ⁹ / g, Enterococcus faecalis (IFO3971) 6 × 10 ⁸ / g, Rhizopus oryzae (IFO4706) 3 × 10 ⁷ / g
(2) a-2: about 50% of carrier (calcium carbonate)
Including Bacillus subtilis (IFO 13719) 5x
10 ⁹ / g, Bacillus licheniformis (IFO12
200) 1 × 10 ⁹ / g, Bacillus polymixer (IF
O3020) Powdered microbial agent containing 1 × 10 ⁹ / g of three genera / species

2. Blowing agent (B) (1) b-1: sodium hydrogen carbonate (2) b-2: sodium carbonate (3) b-3: citric acid (4) b-4: succinic acid Binder (C) (1) c-1: PEG 6000P (finely powdered polyethylene glycol having a molecular weight of 6000, manufactured by Sanyo Chemical Industries, Ltd.) (2) c-2: dextrin 4. Extender (D) (1) d-1: D-sorbitol (2) d-2: sodium sulfate (anhydrous) Additive (E) (1) e-1: talc (lubricant) (2) e-2: calcium stearate (lubricant) (3) e-3: edible blue No. 1 (colorant) (4) e-4: Rose-based powder fragrance (fragrance)

Next, a method for evaluating a tablet will be described. <Test method> 1. Tablet strength The strength of a tablet sample (25 mm in diameter and about 5 g in mass) was measured using a Kiya tablet hardness meter (n = 3), and the average value was shown. 2. Dissolution time One tablet sample (diameter: 25 mm; mass: about 5 g) was put into 500 ml of ion-exchanged water (about 25 ° C.) in a glass beaker, and allowed to stand. (Displayed in units of 5 minutes).

Examples 1 to 5; Comparative Examples 1 and 2 A microbial-containing tablet (25 m in diameter) of the present invention having a mass of about 5 g was prepared on a rotary tableting machine using a powder formulation prepared according to the formulation shown in Table 1.
m) was prepared, and the tablet strength and dissolution time were measured. Also,
No foaming agent was included for comparison with Examples 1 and 2 (the amount corresponding to the foaming agent was prepared by increasing the amount of extender).
Control samples (Comparative Examples 1 and 2) were prepared in the same manner,
The same evaluation as above was performed. Table 1 shows the results.

[0023]

[Table 1]

Use Example 1 A tablet containing a microorganism having a mass of about 100 g was obtained by tableting using a powdered raw material having the same formulation as in Example 1. Next, the obtained tablets were placed at a rate of one tablet / week in a grease strap (cleaned) of a cafeteria used by about 400 people a day, and the test was continued for two months. As a result, on the second day after the introduction, the grease trap was free of the unpleasant odor peculiar to the sewage observed before the test, and the water quality became clean with increased transparency. Further, even after two months, the wall surface of the grease strap was clean without the attachment of dirt such as oil solidified in the form of jelly.

Use Example 2 One tablet (weight: about 5 g) of Example 2 was put into a water tank of a flush toilet twice / weekly. As a result, the dark stains attached to the inner wall surface of the water tank began to fall off four weeks after the introduction, and the odor rising from under the trap recognized before the use of the tablet of the present invention was significantly reduced. I was

As is clear from Table 1, the microbial-containing tablet of the present invention has a significantly shorter time to dissolve in water (1/10 or less) and is easy to handle. Further, as shown in Use Example 1 and Use Example 2, the microorganism-containing tablet of the present invention is excellent in the effect of deodorizing unpleasant odors such as sewage and toilets, and in preventing oil from solidifying.

[0027]

EFFECTS OF THE INVENTION The microorganism-containing tablet of the present invention is not only excellent in degradability and deodorant properties of organic pollutants inherently possessed by microorganisms, but also has a high solubility in water and a conventional powder. Because the powder does not flutter during handling like a microbial agent,
Even people without microbial expertise can handle safely and easily. Therefore, the microorganism-containing tablet of the present invention is useful for applications such as purification and deodorization of kitchen drainage such as grease traps in restaurants, deodorization of septic tanks and flush toilets, deodorization of kitchen sinks in general households and drains of bathrooms. It is.

Claims (4)

[Claims] 1. A microorganism-containing tablet obtained by tablet-forming a powdery or granular raw material comprising a microbial agent (A), a foaming agent (B), and a binder (C). 2. The tablet according to claim 1, wherein the microbial agent (A) comprises a bacterium belonging to the genus Bacillus and contains the microbial agent (A) in an amount of 10% by mass or more. 3. The tablet according to claim 1, wherein the foaming agent (B) comprises two components of a carbonate and an acid. 4. The binder (C) having a molecular weight of 2,0
The tablet according to any one of claims 1 to 3, wherein the tablet is a polyethylene glycol of 00 to 10,000.