JP2001224365A - Combined microbial agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a combined microbial formulation that comprises a microorganism formulation that is safe, harmless and environmentally friendly and can remove microbicidal sliminess and another microorganism formulation that can quickly deodorize the toilet or in the deep position of the drainage pipe and can retain the odorless conditions according to the microorganism. SOLUTION: A powdery microorganism agent that includes the microorganism belonging to Bacillus that produce amylase, protease and lipase is combined with sodium hydrogen carbonate and glucose, or with sodium hydrogen carbonate and alum as bases to give the objective microbial formulation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microbial compounding agent capable of removing bad smell and slime generated in a drain of a toilet or a sink.

[0002]

2. Description of the Related Art Offensive odors generated at the drains of toilets and sinks include odors attached to the surface and odors from pipes clogged. In all cases, proteins and fats contained in the washed sewage are spoiled, which often causes odor. There is a liquid type deodorant as such an odor remover, but this type of odor remover only hits the surface of the outlet of the odor, and is disadvantageous in that it is neutralized on the way and does not reach the odor. Conventionally, preparations for removing slime have been mainly chlorine-based preparations, which require careful handling, and have problems such as safety and environmental pollution.

[0003]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and is intended to provide a safe, harmless and environmentally friendly slime-removing microbial compounding agent and a deep-drain pipe for toilets and sinks. It is an object of the present invention to provide a microbial combination agent that quickly eliminates a certain odor and maintains an odorless state by microorganisms.

[0004]

Means for Solving the Problems The microorganism combination of the present invention made to achieve the above object is a microorganism belonging to the genus Bacillus, which is a microorganism producing amylase and a microorganism producing protease. And a microorganism combination agent containing at least two kinds of microorganisms selected from lipase-producing microorganisms and sodium bicarbonate.

The Bacillus bacterium is an aerobic bacterium that can grow in the presence of oxygen, but uses a strain that can grow well in a place where the oxygen concentration is low.

[0006] Among these strains, strains having excellent enzyme productivity such as amylase, protease and lipase are preferable.

[0007] The Bacillus bacterium is put into a dormant state by fermentation and cell shrinkage of a purely cultured strain on a powdered carrier sterilized.

[0008] The microbial combination agent further contains alum as a base for the purpose of neutralizing odor. Glucose is included as a nutrient in any combination.

[0009]

[Action] Microorganisms of the genus Bacillus that produce enzymes such as amylase, protease, and lipase, and those containing sodium bicarbonate as a base, instantly kill slime bacteria by the astringent action of sodium bicarbonate. At the same time, the microorganisms which decompose dirt adhere to the portion at the same time as collecting and killing them, and the subsequent propagation prevents bad bacteria which generate a bad smell from growing.

[0010] A microorganism containing Bacillus spp. Which produces enzymes such as amylase, protease, and lipase, and a base containing fast-dissolving sodium bicarbonate as well as alum, which has an adhering property and is slowly dissolved, When applied to a dirty place where water is present and produces an odor, first of all, neutralization reaction between ammonia etc. and alum quickly eliminates odors and is suitable for activating microbial bacteria due to the presence of sodium bicarbonate. Na PH (6.0-8.0)
Microorganisms that have been adjusted to a low level and adhered to the soiled place begin to proliferate thereafter, decompose the soil, and maintain the deodorizing effect continuously.

Since the microbial compound of the present invention is of a powder type, it can reach the odor deep in the pipe by using the flow of water, adhere and decompose, and eliminate odor.

[0012] Glucose functions as a nutrient that activates the action of microbial bacteria that have awakened from the dormant state.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION The microbial preparation of the present invention can be used, for example, in the form of a powdered microbial agent containing a bacterium belonging to the genus Bacillus which produces enzymes such as amylase, protease and lipase.
It is obtained by mixing sodium bicarbonate as a base. In the said microbial combination agent, glucose may be contained and the microbial agent may contain a surfactant, a fungus that degrades cellulose, and a lipase enzyme. It can also be obtained by mixing sodium bicarbonate and alum as bases with a powdered microbial agent containing a bacterium belonging to the genus Bacillus which produces enzymes such as amylase, protease and lipase. In the microbial combination, the microbial agent may include a lipase enzyme.

The aforementioned microbial agents are available from the US ATCC (Am
erican Type Culture Colle
The abbreviation of ction) is purely cultured for each strain, and DNA analysis is carried out for each production lot, and bacteria having the same properties are always spread on a powdery carrier, fermentation, cell shrinkage are performed, and a dormant state is obtained. Quality stabilized and powdered. The above-mentioned powdery carrier is in a powdery form and supports the growth of bacteria during culturing and carries the bacteria, and examples thereof include rice bran and glucose. The dormant microorganisms start to proliferate and generate enzymes when they contain water.

Among the microorganisms belonging to the genus Bacillus used in the present invention, for example, Bacillus subtilis (Bacill) is a microorganism which produces amylase or protease.
ussubtilis, ATCC 55405),
As a lipase-producing microorganism, Bacillus lichniformi
s, ATCC54406).

[0016] The above-mentioned microbial combination agent is, for example, three types of microorganisms producing lipase, protease and amylase are used in places soiled with fat, protein and starch. The type and amount of the fungus are changed at any time.

A microorganism of the genus Bacillus producing amylase, protease, lipase, etc., and sodium hydrogen carbonate;
The mixing ratio of glucose is 0.1 to 5% by weight of microorganisms,
Sodium bicarbonate 80-90% by weight, glucose 1
The preferred range is 0.5 to 2% by weight of microbial bacteria, 82 to 86% by weight of sodium bicarbonate, and 13 to 17% by weight of glucose.

The lipase enzyme is formulated for decomposing animal and vegetable fats and oils.

The mixing ratio of Bacillus microorganisms producing amylase, protease, lipase, etc., sodium bicarbonate and alum is 10-20% by weight of microorganisms, 43-53% by weight of sodium hydrogencarbonate and 37% of alum. The preferred ranges are 11 to 17% by weight of microorganisms and 45 to 4% of sodium hydrogen carbonate.
8% by weight, alum 38-41% by weight.

[0020] The microorganism combination agent of the present invention is mainly used for
Removal of slime in bathrooms, washrooms, urinals, toilets, septic tanks, etc.
Used for removal of odors and odors.

[0021]

Embodiments of the present invention will be described below in detail.

Example 1 Bacillus subtilis (Ba) stored in liquid nitrogen
C. subtilis, ATCC 5540
5) and Bacillus litiniformis (Bacillus)
lichniformis, ATCC 55406). This strain is liquid-cultured in a flask.
Check the bacterial account here.

To make a powder formulation, rice bran or glucose is placed in a rotary (rotatable) sterilizer and
After sterilizing at 00 ° C. for 20 minutes or more, the bacteria cultured in the liquid are added and mixed. Mixed bacteria and rice bran,
Or put the bacteria and glucose powder in a tray,
Next, it is put into a fermentation apparatus whose temperature and humidity can be controlled. Raise the temperature and humidity to grow the bacteria on the powder in the tray. After the cultivation, when the bacterial quantity passes the quality standard, it is air-dried, the temperature is lowered, the bacterial cells shrink, and the bacterial growth is stopped (keeping dormant). The powdered product containing the solidified bacteria is ground in a grinder and ground.

A microbial compounding agent was obtained by mixing 16.75 g of sodium bicarbonate and 3 g of glucose with 0.25 g of the microbial agent of this powder. The microbial combination was suitable for removing slime from kitchen sinks.

EXAMPLE 2 7 g of sodium hydrogen carbonate and 6 g of alum were mixed with 2 g of the powdered microbial agent produced in the same manner as described above to obtain a microbial compounding agent. The microbial combination was effective in deodorizing toilets and sinks.

An ammonia water deodorizing test was carried out under the following conditions.

Test Method 1 g of a microbial compounding agent was put in 100 cc of water, and stirred for 30 minutes. 0.5 mL of supernatant water of a tap water biodeodorizer was added to 1 mL of ammonia water (400 ppm). The ammonia concentration was measured after 5 minutes using a detector tube.

As a result, the deodorizing effect was 45%. Similarly, the deodorizing effect of a commercially available deodorant of another company was 32.5%.

Further, a hydrogen sulfide deodorizing test using the compounding agent of the microorganism was performed under the following conditions.

Test method Hydrogen sulfide concentration 100 ppb in 1 liter tetrapack
(Parts per billion) were prepared and the following tests were performed. 1. Blank: Inject 10 mL of distilled water and shake for 1 minute. 2. 1 g of the microorganism combination agent is put into 100 cc of water, and after stirring for 30 minutes, 10 mL of supernatant water is injected and shaken for 1 minute. One minute later, measurement was performed by the FPD-gas chromatography method.

Results 1, Blank: 69 ppb 2, Combination of microorganisms: 2 ppb

[0032]

As described in detail above, the microbial compound of the present invention is a deodorant that utilizes microorganisms in the natural world and does not use chemicals that destroy the environment.
Harmless and environmentally friendly. In addition, by use, the odor can be quickly eliminated and microbial bacteria can be propagated thereafter to maintain an odorless state.

Claims (4)

[Claims] 1. A microorganism belonging to the genus Bacillus, wherein the microorganism comprises at least two kinds of microorganisms selected from a microorganism producing amylase, a microorganism producing protease, and a microorganism producing lipase. A microbial compounding agent comprising sodium hydrogen. 2. The bacterium of the genus Bacillus is a dormant state obtained by spraying a strain cultured on a sterilized powdery carrier, fermenting and shrinking cells. The microbial combination according to the above. 3. A microbial composition comprising the alum in the microbial composition of claim 1 or 2. 4. A microbial composition comprising glucose in the microbiological composition according to claim 1, 2 or 3.