CN108865917A - A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application - Google Patents
A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application Download PDFInfo
- Publication number
- CN108865917A CN108865917A CN201710320168.4A CN201710320168A CN108865917A CN 108865917 A CN108865917 A CN 108865917A CN 201710320168 A CN201710320168 A CN 201710320168A CN 108865917 A CN108865917 A CN 108865917A
- Authority
- CN
- China
- Prior art keywords
- parts
- weight
- complex microorganism
- bacillus
- mildew agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/50—Sulfur oxides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/72—Organic compounds not provided for in groups B01D53/48 - B01D53/70, e.g. hydrocarbons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to microorganism field, a kind of specifically complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application.By 1-5 parts of spherical lysine bacillus, 1-5 parts of bacillus thuringiensis, 150-250 parts of saccharomycete, 300-450 parts of photosynthetic bacteria and the manure of livestock and poultry mixing crossed by 60 DEG C~150 DEG C of high-temperature process, in 36-38 DEG C culture 6-8 days.The present invention by screening multiple beneficial microorganism, with its mutually not antagonism, the feature that cooperates with expands numerous culture mutually, the microbial inoculum is in microbial deodorant, fruits and vegetables cleaning, aquaculture, mould proof field with significant effect.
Description
Technical field
The invention belongs to microorganism field, specifically a kind of complex microorganism deodorization, anti-mildew agent and its preparation side
Method and application.
Background technique
With the continuous development of living standards of the people, the wastewater and waste materials that the daily production and living of resident generate gradually increase, and support
Field, rubbish heap, contaminated water body are grown, always there is the foul gas constantly spread, foul gas directly affects the life of the common people around
Bioplasm amount, or even endanger the health of the people.In addition, the pursuit with resident to quality of the life, the apparatus in toilet is increasingly
It is more, such as bathtub, toilet seat, public place urinal, hand washing sink, supporter, washing machine etc., it is easy to produce daily cleaning dead angle,
Desired thorough cleaning is extremely inconvenient time-consuming and laborious, generates dirt over time, and the peculiar smell in toilet influences the daily life of people
It is living, and pest is bred, quality of life generation is seriously affected.
Traditional elimination peculiar smell method, the mode often using essence covering smell or being evacuated with physics, or use ozone oxidation
Peculiar smell is decomposed, or chemically.Although peculiar smell can be made to obtain a degree of alleviation, it finally can not these methods yet
It fundamentally really solves the problems, such as, furthermore there is also the possibility of secondary pollution for essence and chemical deodorizing, if improper use is very
To being harmful to the human body, for example concentrated hydrochloric acid is used in conjunction with 84 thimerosals and causes human casualty accident commonplace.
Space of the kitchen as processing food, oil smoke is attached to kitchen everywhere when cooking, and water consumption big comparison in kitchen is damp
It is wet, it is also easy to cause mould growth, causes kitchen wall surface to change colour over time and breed pest.In addition, the parlor of most families
Middle raising ornamental fish goes the algae and Other Waste impurity in water removal to keep water quality, builds one completely to ornamental fish
Comfortable environment, cleaning fish jar is essential engineering.At present the way of most people be it is diligent change water, it is diligent clean fish jar, and
And use the chemical cleaners such as glass cleaner.This mode easily causes ornamental fish frequently to replace environment and leads to disease, very
To ornamental fish death.
In recent years, curbed environmental pollution using microbial technique caused the extensive of people note that its efficiently, it is economical and
Advantage without secondary pollution, makes it have more promotional value than physical method and chemical method.Utilize microbial deodorant, fruit-vegetable clear
It washes, aquaculture and mould proof field etc. also increasingly allow people to approve to receive.
Chinese patent 105624072A discloses a kind of fowl and animal excrement deodorant activities microbial inoculum and preparation method thereof.The biological bacteria
Agent has used bacillus subtilis, bacillus cereus, arthrobacterium, streptomycete, saccharomycete, streptococcus lactis, and uses YPD
Culture medium culture, YPD culture medium include 1% yeast extract, 2% peptone, 2% D-glucose.
The fowl and animal excrement deodorant activities microbial inoculum that the patent application provides utilizes the association between six kinds of dejection degradation bacterium/deodorization bacterium
Same-action has the characteristics that bacterial strain combination is new, structure is steady, enzyme activity is strong, bacterium number is high, while solving at traditional Composting
The problems such as reason needs fecaluria separation, dejection degradation is slow, deodorizing effect is unobvious, improves the processing speed and utilization of fowl and animal excrement
Rate, the excrement by deodorizing process can also do organic fertilizer application into farmland, increase soil fertility, and have good ecological benefits
And economic benefit.Its simple process of composite bacteria agent provided by the invention, and the protective agent used in preparation process is non-poisonous material
Matter can guarantee the safety of microbial inoculum.
But the technology culture matrix manufacturing is complicated, and large-scale industrial production is at high cost, is not easy to promote the use of, and is using
Cheng Zhong after fowl and animal excrement deodorant activities microbial inoculum is added into septic tank, is supplied oxygen using mobile air blower, leads to foul gas
It overflows, deodorization effectively can not be carried out to pollution sources.
Currently, mostly using chemical cleaner and frequent cleans for the toilet deodorization prior art to reach holding toilet
The purpose of free from extraneous odour.Chinese patent 104946544A discloses a kind of for domestic waste, public restroom, livestock culture
The microbial bacterial agent of the deodorization of field, including candida utili, lactobacillus plantarum, streptococcus lactis, brewer's yeast, thermophilus
Bacterium, bacillus licheniformis, bafillus natto.
It, can be rubbish by synergistic effect between the beneficial microbe contained in the deodorization composite bacteria agent of the invention
Field, public restroom, the corruption of spoilage organisms degrades and decomposes transformation to fermentation in fowl and livestock farm, to the effluvium generated
Body hydrogen sulfide and ammonia absorb degradation, eliminate the stench of rubbish and landfill leachate, while beneficial bacterium lactobacillus plantarum can produce
Lactogenesis acid inhibits the growth of putrefactivebacteria, and from the basic generation for solving foul gas, stink and disease-free can be eliminated by forming one
Evil propagates dumping environment.
But contains brewer's yeast in the microbial inoculum, alcohol and carbon dioxide are generated in oxygen-free environment, carbon dioxide is in water
Use environment, i.e., can not be acidified by the lactic acid that may neutralize lactobacillus plantarum, streptococcus lactis and streptococcus thermophilus production.Its
Environment-adapting ability is poor, and deodorizing effect is weaker.
Chinese patent 104560811A discloses a kind of for cultivating the complex micro organism fungicide of water process, and the microbial inoculum is by wrapping
Bafillus natto, lactic acid bacillus and bacillus coagulans are included.It in use, can be straight by complex micro organism fungicide
It connects dispensing in water, into composite bacteria in cultivation water through growth and breeding after a period of time, can produce metabolite, purified water
It can also be used as feed while matter, aquatic products is made to be not easy to fall ill, improve quality.
Though the microbial inoculum can play the function of purifying aquaculture water, microbial inoculum effective time in water body is short, used
Culture medium is the fermentation medium manually prepared, and the production of the culture medium still will cause pollution, and environment is unfriendly.
Summary of the invention
Technical problem to be solved by the present invention lies in providing a kind of complex microorganism deodorization, anti-mildew agent, and further
Open preparation method.
Complex microorganism deodorization, anti-mildew agent, which is characterized in that the effective component including following parts by weight：
Spherical lysine bacillus 1-5 parts by weight, bacillus thuringiensis 1-5 parts by weight.
Further, complex microorganism deodorization, anti-mildew agent, the effective component including following parts by weight：
Spherical lysine bacillus 1-5 parts by weight, bacillus thuringiensis 1-5 parts by weight, saccharomycete 150-250 weight
Part, photosynthetic bacteria 300-450 parts by weight；
It is preferred that the effective component of following parts by weight：Spherical 2 parts by weight of lysine bacillus, 2 weight of bacillus thuringiensis
Measure part, 200 parts by weight of saccharomycete, 300 parts by weight of photosynthetic bacteria.
Further, the saccharomycete is Angel Yeast；The photosynthetic bacteria is eel treasured photosynthetic bacteria.
The complex microorganism deodorization, anti-mildew agent method, it is characterised in that：Include the following steps：
(1) according to selected bacterial strain, preparation is suitable for the spherical lysine bacillus, bacillus thuringiensis, ferment
The culture medium that female bacterium, photosynthetic bacteria are cultivated；
(2) a selected amount of spherical lysine bacillus, Su Yun gold bud bacillus, saccharomycete, photosynthetic bacteria is taken to be added
In the culture medium, cultivated in 36-38 DEG C to get the complex microorganism deodorization, anti-mildew agent.
Further, in the step (1), the culture medium is matched by the manure of livestock and poultry that 60 DEG C~150 DEG C of high-temperature process is crossed
It is made, whole microbial bacterias and the culture medium mass ratio are 0.01~0.1 in microbial inoculum：1.
Further, the specific preparation steps of the culture medium include：Take selected manure of livestock and poultry pulvis in 30 DEG C~50 DEG C
Under the conditions of add water to humidity be 20%~40% culture obtain.
Further, the manure of livestock and poultry is selected from least one of cow dung, pig manure, chicken manure.
Further, in the step (2), further include the steps that by selected bacterial strain be suitable for activating in activating solution；
Preferably, the activating solution of the activation step is identical as the culture medium.
Complex microorganism deodorization that the method is prepared, anti-mildew agent.
Above-mentioned complex microorganism deodorization, anti-mildew agent fruits and vegetables cleaning, aquaculture, it is mould proof in application.
The addition microbial inoculum in moisture is only needed to spray to object or mix microbial inoculum with lotion the present invention provides a kind of
To object smear, no pollution to the environment is harmless, and continuous and effective can pre- mould proof and deodorization.
The invention has the advantages that：
1. the present invention utilizes microbial deodorant, mould proof technology, mutual not using them by screening multiple beneficial microorganism
Antagonism expands numerous culture under the action of collaboration mutually, applies the deodorization, mould proof in polluted-water, pipeline and textile field
Microbial bacterial agent.
Using the odorant that can convert or degrade peculiar microorganism flora decomposition, absorption and metabolism to water
The foul gas such as hydrogen sulfide, sulfur dioxide, ammonia, nitrogen, phosphorus, carbon disulfide, methane that body, sewer pipe etc. distribute and nuisance
Matter, pathogen etc. are purified, and are decomposed, and harmless odorless substance is converted into, and reduce foul gas and harmful substance, pathogen etc.
To water body to the pollution of air, reduction processing difficulty and expense.Reaching thoroughly improves water quality, environment purification, protection human health
Purpose.
2. being reduced using fowl and animal excrement as culture medium in the preparation process of complex microorganism deodorizing fungus-proof microbial inoculum of the invention
Cost, preparation process are more efficient.
3. can grow and be metabolized in completely inorganic environment using photosynthetic bacteria in the present invention, they can be by inorganic dirt
Contaminate object such as H2S、NH3Equal substances are changed into containing prot th and become the component part of neonatal cell, or are further converted to low
The NO of toxicity3 +And molecular sulfur.Meanwhile photosynthetic bacteria also reduces CO in air using photosynthesis2Content, so as to improve sky
The quality of gas.Photosynthetic bacteria in water body, can quickly and effectively by the odorants such as ammonia and hydrogen sulfide be oxidized to it is odorless nontoxic and
The nitrate of non-volatility contains sulfoprotein and microbial cell matter.Therefore, more can by the foul smell that the substance release of nitrogenous sulfur-bearing goes out
Effectively controlled.And utilize the Environmental adaptation of Bacillus sphaericus and strength deodorization, inhibit pathogen ability etc..Greatly
The catabolic enzyme for measuring beneficial microbe and its generation, can make in water body or sewer pipe (including；Primary pollution sources) generate ammonia
The organic matters such as nitrogen, hydrogen sulfide, mercaptan, methane, phosphorus decompose, and harmful microorganism is made to be inhibited, and it is numerous beneficial to mushroom to carry out duration
It grows, degradation of contaminant, deodorization and the contaminated water quality of purification reduce dirt.Multiple-microorganism collective effect is more advantageous to suction
The pernicious gas receiving, decompose water body, having foul smell in sewer pipe.
4. complex microorganism deodorizing fungus-proof microbial inoculum of the invention is pulvis, preparation method is simple, at low cost, can with it is independent
Using being used after can also being mixed with carriers such as liquid, lotion, water absorbent gels, using wide variety, simply.
5. the effect that the microorganism in the microbial inoculum can produce antibacterial again.Without chemical addition agent, secondary pollution, nothing are not generated
Secondary disaster repairs original ecological environment, improves self-purification capacity.
Detailed description of the invention
Fig. 1 is purifying aquaculture water quality experiment schematic diagram, wherein left side is 1 schematic diagram of comparative example,
Right side is 1 schematic diagram of experimental example.
Fig. 2 is that simulation removal mould tests schematic diagram, wherein left side is that aspergillus niger is tested, right side is Penicillium citrinum experiment.
Fig. 3 is mould prevention schematic diagram.
Fig. 4 is to remove closestool to urinate alkali experiment schematic diagram, wherein Fig. 4 (a) is the 0th day fouled state of experiment, and Fig. 4 (b) is real
The 7th day fouled state is tested, Fig. 4 (c) is fouled state before scrubbing for experiment the 20th day, and Fig. 4 (d) is dirt after experiment scrub in the 20th day
State, Fig. 4 (e) are the 42nd day fouled state of experiment, and Fig. 4 (f) is the 60th day fouled state of experiment.
Specific embodiment
Spherical shape lysine bacillus of the present invention is purchased from CGMCC：(number：1.270), bacillus thuringiensis
Purchased from CGMCC (number：1.785)；
For the yeast used for Angel Yeast, photosynthetic bacteria is eel treasured photosynthetic bacteria.
Embodiment 1
1, it is standard by 150kg tank in aseptic production department, opens temperature control system, culture tank is made to rise to 37 DEG C.(temperature control system
Temperature of uniting control is at 37 DEG C)
2, the 100kg chicken manure using high-temperature sterilization pot after 100 DEG C of sterilizings is added in culture tank.Open the stirring of fermentor
System is stirred 10 minutes；Obtain culture medium.
3, the drain hole for opening fermentor is released 1kg culture medium and is mixed with 2kg water as activating solution, and actication of culture is used for,
2000g Angel Yeast bacteria powder is added in 1.2kg activating solution.Take addition 3000g eel treasured photosynthetic bacteria powder in 1.2kg activating solution
Agent.Spherical lysine bacillus 20g, bacillus thuringiensis 20g are uniformly mixed with 300g activating solution respectively.This step is used for
Activated spawn.
4, activated saccharomycete, photosynthetic bacteria, spherical lysine bacillus, bacillus thuringiensis are sequentially added
Fermentation in culture tank, adding water to humidity in culture tank is 25%.
5, it opens stirring system to stir 15 minutes, opens temperature control system, temperature control is produced in 37 DEG C of culture completions in 7 days.(note：7
Daily early, middle and late stirring system of opening respectively stirs 15 minutes in it).
6, it detects：PH is between 6-7, beneficial viable count：Every gram 500,000,000 or more.
7, it is packed using sealed in unit.
Embodiment 2
It is saccharomycete 1500g, photosynthetic bacteria 4000g, spherical lysine bacillus 50g, Su Yun with 1 difference of embodiment
Golden bacillus 50g, manure of livestock and poultry are cow dung.
Embodiment 3
It is saccharomycete 2500g, photosynthetic bacteria 4500g, spherical lysine bacillus 10g, Su Yun with 1 difference of embodiment
Golden bacillus 10g.
Experimental example 1
The complex microorganism deodorization for preparing according to the method for embodiment 1, anti-mildew agent 10g, it is useless with 300g cinder or building
Material is sufficiently mixed, and is pressed into water purification stone.Water purification stone is placed in the fish jar that volume is 10L, is added and places in the sun 1 day
Tap water 8L, after be put into fancy carp 2.Every daily 5 fishes of fish eat, the sundries such as every 3 days cleaning fish excrement and fish food, right column in attached drawing 1
Water quality photo when being from top to bottom respectively the 0th day, the 10th day, the 20th day, the 27th day；As shown in Figure 1, after being put into water purification stone,
Fish jar water quality is without significant change in 27 days, and as clear as crystal, fish jar inside has no that microorganism adheres to caused fish jar transparency and reduces.
For fancy carp without obvious illness symptom, activity is normal.
Comparative example 1
Try to please in the fish jar that product is 10L, be added and place 1 day tap water 8L in the sun, after be put into fancy carp 2.Every fish
Daily 5 fishes food, the sundries such as every 3 days cleaning fish excrement and fish food, left column from top to bottom respectively the 0th day in attached drawing 1, the 10th day,
Water quality photo at the 20th day, the 27th day；As shown in Figure 1, fish jar inner glass transparency gradually decreases, water quality is gradually muddy,
Two articles of fancy carps are all dead within 27th day.
Experimental example 2
Simulation removal mould experiment
Experimental strain：Aspergillus niger is selected (to be purchased from CGMCC, deposit number：3.6478) and Penicillium citrinum (is purchased from CGMCC, preservation
Number：3.825) it is used as experimental strain.
Experimental method：Each inoculated aspergillus niger and Penicillium citrinum in two plating mediums, will be by the made bacterium of 3 method of embodiment
Agent takes 1g to be applied on wet filter paper as sample, sample is placed in plating medium center, at 21 DEG C ± 1 DEG C
It is cultivated in environment, observes sample to the growth inhibitory effect of mould, and record the width in growth inhibition area.
For experimental result as shown in Fig. 2, left side is that aspergillus niger is tested in Fig. 2, right side is Penicillium citrinum experiment.
Table 1：Mould fungus inhibition effect statistical form
Experimental example 3
Mould prevention
Experimental strain：1, aspergillus niger (is purchased from CGMCC, deposit number：3.6478), 2, Penicillium citrinum (is purchased from CGMCC, preservation
Number：3.825), 3, ball hair shell (is purchased from CGMCC, deposit number：3.9465), 4, myrothecium verrucaria (is purchased from CGMCC, preservation is compiled
Number：3.219)
Experimental method：Using inorganic salts agar medium, wet filter will be applied to by 3 method institute bacteriostat 1g of embodiment
It is used as sample on paper, sample is fitted tightly on culture medium, by mixing spore suspension (the suspension spore of four kinds of experimental strains
Subnumber is respectively 1~2 × 105A/mL) culture medium to be sprayed, culture environment is 28 ± 2 DEG C, after culture 14 days, observes mould
Growing state.
Experimental result on sample from can be seen that in following table without experimental strain as shown in figure 3, grow.According to 1 side of embodiment
It is good that microbial inoculum obtained by method preparation puts mould effect.
Table 2：Prevent fungus growth effect statistical form
In table：- without fungus growth
± mould slightly grows
+~mould continuously significantly increases
0 indicates no mycelia growth
Within the 1/3 of 1 growth covering sample area
2/3 or more of 2 growth covering sample areas
Experimental example 4
Remove closestool urine alkali experiment
Fig. 4 (a) is the 0th day fouled state of experiment, and Fig. 4 (b) is the 7th day fouled state of experiment, and Fig. 4 (c) is experiment the 20th
Fouled state before it is scrubbed, Fig. 4 (d) are fouled state after experiment scrub in the 20th day, and Fig. 4 (e) is the 42nd day fouled state of experiment,
Fig. 4 (f) is the 60th day fouled state of experiment.
It takes a normal use 4 months but does not make clean closestool, as shown in Fig. 4 (a), closestool inner wall is attached in water
Yellow urine examination.Pungent urine stink can be smelt on the 0th day by testing.During the experiment in addition to the microbial inoculum that embodiment 2 is prepared, do not make
With any other cleaning agent or biological agent.
After microbial inoculum is put into closestool 7 days, as shown in Fig. 4 (b), the urine alkali of closestool inner wall softens, and pungent urine stink obtains
Alleviate.
After microbial inoculum is put into closestool 20 days, as shown in Fig. 4 (c), the urine alkali of closestool inner wall is gently scrubbed with toilet brush then may be used
Brush off 60%.Stink is urinated to mitigate.As a result as shown in Fig. 4 (d).
After microbial inoculum is put into closestool 42 days, as shown in Fig. 4 (e), closestool inner wall urinates alkali and only remains 20% or so, anuria stink.
After microbial inoculum is put into closestool 60 days, as shown in Fig. 4 (f), closestool inner wall urinates alkali and all disappears.Anuria stink.
Experimental example 5
Experimental material is the deodorizing fungus-proof microbial inoculum prepared in embodiment 1 in this experimental example.
1, sulphur removal sour water stink is tested
Table 3：Sulphur removal sour water stink experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 4.0 | 4.0 |
| After 2 hours | 3.7 | 4.0 |
| After 4 hours | 2.2 | 4.0 |
Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Aqueous sulfuric acid 4PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
2, ammonia is tested
Table 4：Except ammonia smell experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 100 | 100 |
| After 2 hours | 0.2 | 100 |
| After 4 hours | Less than 0.2 | 65.0 |
Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Ammonia 100PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
3、Methyl mercaptanExperiment
Table 5：Except methyl mercaptan smell experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 8.0 | 8.0 |
| After 2 hours | 7.5 | 8.0 |
| After 4 hours | 7.0 | 8.0 |
※：Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Methyl mercaptan8PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
4、TrimethylamineExperiment
Table 6：It removesTrimethylamineSmell experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 4.0 | 4.0 |
| After 2 hours | 3.7 | 4.0 |
| After 4 hours | 2.2 | 4.0 |
Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Methyl mercaptan4PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
5, acetic acid is tested
Table 7：Except acetic acid smell experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 50.0 | 50.0 |
| After 2 hours | 0.2 | 50.0 |
| After 4 hours | 0.1 | 27.5 |
Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Acetic acid 50PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
6, Formaldehyde Test
Table 8：Except formaldehyde odor experiment statistics table
| Experimental group (PPM) | Control group (PPM) | |
| After 0 minute | 14.0 | 14.0 |
| After 2 hours | 9.0 | 14.0 |
| After 4 hours | 6.0 | 14.0 |
※：Test method is as follows：
The weight of experimental material：10g (microbial inoculum：8g, water：2g mixing)
Experiment container：5 liters of Taide pulling bags
Gas flow in container：3 liters
Gas initial stage concentration：Formaldehyde 14PPM
Eudiometry method：Detection tube method
Test room temperature：20℃
Control group：Without experimental material, operate identical as experimental group.
Claims (10)
1. a kind of complex microorganism deodorization, anti-mildew agent, which is characterized in that the effective component including following parts by weight：
Spherical lysine bacillus 1-5 parts by weight, bacillus thuringiensis 1-5 parts by weight.
2. complex microorganism deodorization, anti-mildew agent according to claim 1, which is characterized in that including having for following parts by weight
Imitate ingredient：
Spherical lysine bacillus 1-5 parts by weight, bacillus thuringiensis 1-5 parts by weight, saccharomycete 150-250 parts by weight,
Photosynthetic bacteria 300-450 parts by weight；
It is preferred that the effective component of following parts by weight：Spherical 2 parts by weight of lysine bacillus, 2 parts by weight of bacillus thuringiensis,
200 parts by weight of saccharomycete, 300 parts by weight of photosynthetic bacteria.
3. complex microorganism deodorization, anti-mildew agent according to claim 1, it is characterised in that：The saccharomycete is Angel ferment
It is female；The photosynthetic bacteria is eel treasured photosynthetic bacteria.
4. a kind of method for preparing any one of claim 1-3 complex microorganism deodorization, anti-mildew agent, it is characterised in that：
Include the following steps：
(1) according to selected bacterial strain, preparation be suitable for the spherical lysine bacillus, bacillus thuringiensis, saccharomycete,
The culture medium of photosynthetic bacteria culture；
(2) it takes described in a selected amount of spherical lysine bacillus, Su Yun gold bud bacillus, saccharomycete, photosynthetic bacteria be added
In culture medium, cultivated in 36-38 DEG C to get the complex microorganism deodorization, anti-mildew agent.
5. the method according to claim 4 for preparing complex microorganism deodorization, anti-mildew agent, which is characterized in that the step
Suddenly in (1), the culture medium is prepared to obtain by the manure of livestock and poultry that 60 DEG C~150 DEG C of high-temperature process is crossed, whole microorganisms in microbial inoculum
Bacterium and the culture medium mass ratio are 1~10：100.
6. the method according to claim 5 for preparing complex microorganism deodorization, anti-mildew agent, which is characterized in that the training
Support base specific preparation steps include：Take selected manure of livestock and poultry pulvis added water under the conditions of 30 DEG C~50 DEG C humidity be 20%~
40% culture obtains.
7. the method according to claim 5 or 6 for preparing complex microorganism deodorization, anti-mildew agent, which is characterized in that described
Manure of livestock and poultry be selected from least one of cow dung, pig manure, chicken manure.
8. according to the described in any item methods for preparing complex microorganism deodorization, anti-mildew agent of claim 4-7, feature exists
In, in the step (2), further include the steps that by selected bacterial strain be suitable for activating solution in activate；Preferably, the activation
The activating solution of step is identical as the culture medium.
9. the complex microorganism deodorization being prepared by the described in any item methods of claim 4-8, anti-mildew agent.
10. complex microorganism deodorization, anti-mildew agent described in any one of claim 1-3 or claim 9 are in fruits and vegetables cleaning, aquatic products
Application in cultivating, being mould proof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710320168.4A CN108865917A (en) | 2017-05-09 | 2017-05-09 | A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710320168.4A CN108865917A (en) | 2017-05-09 | 2017-05-09 | A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108865917A true CN108865917A (en) | 2018-11-23 |
Family
ID=64287378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710320168.4A Pending CN108865917A (en) | 2017-05-09 | 2017-05-09 | A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108865917A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536408A (en) * | 2018-12-10 | 2019-03-29 | 福建农林大学 | A kind of complex microorganism bacterium solution and its application |
| CN110042071A (en) * | 2019-04-02 | 2019-07-23 | 辽宁石油化工大学 | A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180546A (en) * | 2011-03-24 | 2011-09-14 | 上海大微生物科技有限公司 | Concrete stone block for water quality purification and manufacturing method thereof |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
| CN104120085A (en) * | 2013-04-24 | 2014-10-29 | 刘智云 | Composite microbe deodorizing and degrading inoculant, and preparation method and application thereof |
-
2017
- 2017-05-09 CN CN201710320168.4A patent/CN108865917A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102180546A (en) * | 2011-03-24 | 2011-09-14 | 上海大微生物科技有限公司 | Concrete stone block for water quality purification and manufacturing method thereof |
| CN104120085A (en) * | 2013-04-24 | 2014-10-29 | 刘智云 | Composite microbe deodorizing and degrading inoculant, and preparation method and application thereof |
| CN103992968A (en) * | 2014-04-22 | 2014-08-20 | 安徽清明环保科技有限公司 | Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| JOSEF DEUTSCHER ET AL.: "How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria", 《MICROBIOL MOL BIOL REV》 * |
| 李彪等: "猪粪中除臭微生物的筛选和鉴定", 《家畜生态学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536408A (en) * | 2018-12-10 | 2019-03-29 | 福建农林大学 | A kind of complex microorganism bacterium solution and its application |
| CN110042071A (en) * | 2019-04-02 | 2019-07-23 | 辽宁石油化工大学 | A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104667320B (en) | Composite microbial deodorant for treating household garbage and preparation method of deodorant | |
| CN104388363B (en) | A kind of organic waste deodorization, decrement compound bacteria and preparation method thereof | |
| CN101544959B (en) | Complex probiotics for environmental remediation, preparation method and application thereof | |
| CN101269227B (en) | Biological agent for purifying air, preparation method and uses thereof | |
| CN109528624B (en) | Biological deodorization spray for pets, and preparation method and application thereof | |
| CN108048344B (en) | Two plants of deodorization bacterial strains and its application in preparation composite biological deodorant | |
| KR101534891B1 (en) | Microorganism capable of reducing malodor and its uses | |
| CN109609401A (en) | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof | |
| CN106520627A (en) | Microbial sludge deodorizer and preparation method thereof | |
| CN101665773A (en) | Microbial bacterial agent, preparation method thereof and method for treating animal excrements | |
| CN107325992A (en) | A kind of bacillus subtilis strain and its application | |
| JP2810308B2 (en) | Deodorant containing microorganisms and their cells | |
| CN102813948A (en) | Preparation method of composite biological deodorant | |
| CN107760632A (en) | A kind of complex micro organism fungicide and preparation method thereof | |
| CN108913626B (en) | Livestock and poultry manure deodorizing composite microbial inoculum and preparation method thereof | |
| CN106305487A (en) | Biological active padding material of fermentation bed for poultry, preparation method and application | |
| CN105255761B (en) | A kind of deodorization microbial inoculum and its application method for organic waste treatment | |
| CN109762767A (en) | A kind of sewage complex microorganism inorganic agent and the preparation method and application thereof | |
| US11680003B2 (en) | Treatment of animal and poultry waste to reduce odor | |
| CN106830348A (en) | The oil interceptor deodorizing method of biological agent is combined based on active microorganism | |
| CN108865917A (en) | A kind of complex microorganism deodorizing fungus-proof microbial inoculum and its preparation method and application | |
| CN106915827A (en) | The method for treating garbage percolation liquid of biological agent is combined based on active microorganism | |
| CN109988020A (en) | Aquaculture and planting industry cyclic utilization of waste method | |
| TW201813514A (en) | Biological agents to improve the growth of pigs and farms environment and use thereof | |
| CN109293185A (en) | A kind of henhouse waste deodorant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181123 |