CN110042071A - A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof - Google Patents
A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof Download PDFInfo
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- CN110042071A CN110042071A CN201910263129.4A CN201910263129A CN110042071A CN 110042071 A CN110042071 A CN 110042071A CN 201910263129 A CN201910263129 A CN 201910263129A CN 110042071 A CN110042071 A CN 110042071A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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Abstract
The invention discloses a kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculums and preparation method thereof.The deodorizing microorganism composite bacteria agent includes the following components in terms of parts by volume: the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, the bacterium solution of bacillus thuringiensis, bacterium solution part of bacillus, mould bacterium solution part.It is beneficial in that, (1) microbial inoculum microorganism can well grow in waste, occupy advantageous ecological niche, it is suppressed that produce stench microbial growth, reduce stench release strength, realize the control of stench original position source.(2) efficient combination for realizing bacterial strain, the microbial inoculum after combination are significantly improved than single bacterium deodorizing effect.(3) meet bio-safety regulation, the bacterial strain for including in microbial inoculum is obtained from being coerced to screen in selection environment for a long time by Animal manure loss, will not be caused damages to ambient enviroment and the ecological balance.(4) use aspect, packaging, transport convenience, easily forms commercialization.
Description
Technical field
The invention belongs to agricultural planting organic fertilizer field, more particularly, to a kind of deodorizing microorganism composite bacteria agent and
Solid union microbial inoculum and preparation method thereof.
Background technique
As chemical fertilizer is largely applied, soil pollution of area source problem is more and more prominent, and crop straw burning, scale animal dung are big
Amount enters water body etc. and has seriously polluted the environment.Therefore, organic fertilizer production is greatly developed, agricultural wastes utilization of resources water is improved
It is flat, it is the important directions of domestic and international new-type fertilizer research.
Biological organic fertilizer can effectively improve chemical fertilizer utilization ratio 5%-10%, and make plant all one's life all can be by beneficial microbe
Protection, inhibit harmful microbe growth and breeding, reduce the generation of disease, promote strong sprout, promote stable high yield.But in reality
During production application, it has been found that the composition and quality of the microbial bacterial agent product of addition and containing for product living bacteria count
There are great differences with external product for amount etc., causes that decomposed time is long, decomposed is not thorough and the smoked people of the place stink that ferments
Phenomena such as.There is physical method in existing stage China in the main method of some foul gas such as processing hydrogen sulfide, ammonia, and chemical method is gone back
There is absorption process etc., but comparatively expense required for these methods is also relatively high.The method of common processing foul smell is at present
Biological deodorization method controls foul smell in composting process, this method efficiency by the way that microorganism is added to the inside in composting process
Height, expense are low.
Summary of the invention
It is an object of the invention to solve above-mentioned technical problem, a kind of deodorizing microorganism composite bacteria agent is provided, it can be efficient
Remove H in muck2S and ammonia shorten the muck decomposed time.Organic fertilizer is prepared for can significantly mention in crop planting
High crop yield, and avoid land fertility reduction, soil hardening caused by abusing because of chemical fertilizer.
To achieve the goals above, the first aspect of the present invention provides a kind of deodorizing microorganism composite bacteria agent, and the deodorization is micro-
Biological compound fungi agent includes the following components in terms of parts by volume:
2-5 parts of bacterium solution, 2-4 parts of the bacterium solution of bacillus cereus, the bacterium solution of bacillus thuringiensis of bacillus licheniformis
1-3 parts, 0-2 parts of the bacterium solution of bacillus, 0-2 parts of the bacterium solution of mould.
As a preferred embodiment of the present invention, the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
Bacterium solution, the bacterium solution of bacillus, the volume ratio of the bacterium solution of mould of bacillus are 10:4:4:1:1.
As a preferred embodiment of the present invention, in the deodorizing microorganism composite bacteria agent, total viable count >=2.5 ×
109CFU/mL。
The second aspect of the present invention provides the preparation method of above-mentioned deodorizing microorganism composite bacteria agent, the preparation method packet
It includes:
By bacillus licheniformis, bacillus cereus, bacillus thuringiensis, bacillus, mould is respectively in YPD solid
It is activated in slant medium, then is inoculated into microbial inoculum fluid nutrient medium and cultivates 20-28 hours respectively, adjusted separately each bacterial strain and exist
Bacteria containing amount in microbial inoculum fluid nutrient medium is 109Then five kinds of bacterium solutions are combined by CFU/mL, the micro- life of deodorization is prepared
Object composite bacteria agent.
As a preferred embodiment of the present invention, the formula of the microbial inoculum fluid nutrient medium are as follows: peptone 5g, fructus hordei germinatus leaching powder
10g, sodium chloride 5g, glucose 20g, water 1000mL, pH6.5-7.0.
The third aspect of the present invention provides a kind of solid union microbial inoculum, wherein the solid union microbial inoculum is by including above-mentioned
Deodorizing microorganism composite bacteria agent be prepared.
The fourth aspect of the present invention provides the preparation method of above-mentioned solid union microbial inoculum, which includes:
0.8-1.2kg adsorbent is added into deodorizing microorganism composite bacteria agent described in 1L, in 38-42 DEG C of constant incubator
Middle drying is cultivated 20-24 hours, until water content is 15-20%, 4-6g promotor is added, stirs evenly, obtains the solid-state and remove
Smelly complex microbial inoculum.
As a preferred embodiment of the present invention, the adsorbent is made of wheat bran, active carbon and diatomite.
As a preferred embodiment of the present invention, the weight ratio of wheat bran, active carbon and diatomite is 2:1:1.
In the present invention, YPD solid slope culture medium formula are as follows:
Peptone 20g, beef extract 10g, glucose 20g, water 1000mL, agar 15-20g, pH6.5-7.0.
As a preferred embodiment of the present invention, deodorizing microorganism composite bacteria agent viable count is more than or equal to 100,000,000 CFU/mL；pH
6-7；Suspension, color are micro- red.
As a preferred embodiment of the present invention, solid union microbial inoculum viable count is more than or equal to 100,000,000 CFU/g；Particle drying,
Surface is milky white.
The invention has the beneficial effects that:
(1) microbial inoculum microorganism can well grow in waste, occupy advantageous ecological niche, it is suppressed that it is raw to produce stench microorganism
Long breeding, reduces stench release strength, realizes the control of stench original position source.
(2) efficient combination for realizing bacterial strain, the microbial inoculum after combination are significantly improved than single bacterium deodorizing effect.
(3) meet bio-safety regulation, the bacterial strain for including in microbial inoculum from being coerced selection ring by Animal manure loss for a long time
Screening obtains in border, will not cause damages to ambient enviroment and the ecological balance.
(4) use aspect, packaging, transport convenience, easily forms commercialization.
Other features and advantages of the present invention will then part of the detailed description can be specified.
Specific embodiment
The preferred embodiment of the present invention is described in more detail below.Although the following describe preferred implementations of the invention
Mode, however, it is to be appreciated that may be realized in various forms the present invention without that should be limited by the embodiments set forth herein.Phase
Instead, these embodiments are provided so that the present invention is more thorough and complete, and can be by the scope of the present invention completely
It is communicated to those skilled in the art.
Bacterium solution in embodiment is obtained by self-control, and Homemade method is the method that those skilled in the art routinely use.
Embodiment 1
A kind of deodorizing microorganism composite bacteria agent, by the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
The bacterium solution of the bacterium solution of bacillus, the bacterium solution of bacillus, mould is formed according to the volume ratio of 10:4:4:1:1.The deodorization
Total viable count >=2.5 × 10 in complex microbial inoculum9CFU/mL。
Embodiment 2
A kind of deodorizing microorganism composite bacteria agent, by the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
The bacterium solution of the bacterium solution of bacillus, the bacterium solution of bacillus, mould is formed according to the volume ratio of 2:4:3:1:1.The deodorization
Total viable count >=2.5 × 10 in complex microbial inoculum9CFU/mL。
Embodiment 3
A kind of deodorizing microorganism composite bacteria agent, by the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
The bacterium solution of the bacterium solution of bacillus, the bacterium solution of bacillus, mould is formed according to the volume ratio of 5:4:3:1:1.The deodorization
Total viable count >=2.5 × 10 in complex microbial inoculum9CFU/mL。
Embodiment 4
A kind of deodorizing microorganism composite bacteria agent, by the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
The bacterium solution of the bacterium solution of bacillus, the bacterium solution of bacillus, mould is formed according to the volume ratio of 4:2:1:1:2.The deodorization
Total viable count >=2.5 × 10 in complex microbial inoculum9CFU/mL。
Embodiment 5
A kind of deodorizing microorganism composite bacteria agent, by the bacterium solution of bacillus licheniformis, the bacterium solution of bacillus cereus, Su Yunjin
The bacterium solution of bacillus is formed according to the volume ratio of 4:2:1.Total viable count >=2.5 in the deodorizing microorganism composite bacteria agent ×
109CFU/mL。
Test case
The deodorizing microorganism composite bacteria agent of embodiment 1 is used for cow dung organic fertilizer fermentation, can significantly shorten the deodorization time,
Removal rate reaches 95% or more, shows that the deodorization bacteria preparation has remarkable result to organic fertilizer fermentation deodorization.
Various embodiments of the present invention are described above, above description is exemplary, and non-exclusive, and
It is not limited to disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for this skill
Many modifications and changes are obvious for the those of ordinary skill in art field.
Claims (9)
1. a kind of deodorizing microorganism composite bacteria agent, which is characterized in that the deodorizing microorganism composite bacteria agent includes in terms of parts by volume
Following components:
2-5 parts of the bacterium solution of bacillus licheniformis, 2-4 parts of the bacterium solution of bacillus cereus, bacillus thuringiensis bacterium solution 1-3
Part, 0-2 parts of the bacterium solution of bacillus, 0-2 parts of the bacterium solution of mould.
2. deodorizing microorganism composite bacteria agent according to claim 1, wherein the bacterium solution of bacillus licheniformis, waxy gemma
Bacterium solution, the bacterium solution of bacillus thuringiensis, the bacterium solution of bacillus, the volume ratio of the bacterium solution of mould of bacillus are 10:4:4:1:
1。
3. deodorizing microorganism composite bacteria agent according to claim 1, wherein in the deodorizing microorganism composite bacteria agent, always
Viable count >=2.5 × 109CFU/mL。
4. the preparation method of deodorizing microorganism composite bacteria agent described in any one of claim 1-3, which is characterized in that the system
Preparation Method includes:
By bacillus licheniformis, bacillus cereus, bacillus thuringiensis, bacillus, mould is respectively in YPD solid slope
It is activated in culture medium, then is inoculated into microbial inoculum fluid nutrient medium and cultivates 20-28 hours respectively, adjust separately each bacterial strain in microbial inoculum
Bacteria containing amount in fluid nutrient medium is 109Then five kinds of bacterium solutions are combined by CFU/mL, it is multiple that deodorizing microorganism is prepared
Combined bacteria agent.
5. the preparation method according to claim 4, wherein the formula of the microbial inoculum fluid nutrient medium are as follows: peptone 5g, wheat
Bud soaks powder 10g, sodium chloride 5g, glucose 20g, water 1000mL, pH6.5-7.0.
6. a kind of solid union microbial inoculum, wherein the solid union microbial inoculum is as including described in any one of claim 1-3
Deodorizing microorganism composite bacteria agent is prepared.
7. the preparation method of solid union microbial inoculum as claimed in claim 6, which is characterized in that the preparation method includes:
0.8-1.2kg adsorbent is added into deodorizing microorganism composite bacteria agent described in 1L, is dried in 38-42 DEG C of constant incubator
Dry culture 20-24 hours is added 4-6g promotor, stirs evenly, it is micro- to obtain the solid-state deodorization until water content is 15-20%
Biological compound fungi agent.
8. preparation method according to claim 7, wherein the adsorbent is by wheat bran, active carbon and diatom local soil type
At.
9. preparation method according to claim 8, wherein the weight ratio of wheat bran, active carbon and diatomite is 2:1:
1。
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| CN201910263129.4A CN110042071A (en) | 2019-04-02 | 2019-04-02 | A kind of deodorizing microorganism composite bacteria agent and solid union microbial inoculum and preparation method thereof |
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Application publication date: 20190723 |