CN102068711A - Microbial deodorant as well as preparation method and application thereof - Google Patents
Microbial deodorant as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial deodorant as well as a preparation method and application thereof. The microbial deodorant comprises bacillus and filamentous fungus. The preparation method comprises the steps of: uniformly mixing bacillus fermentation liquor and filamentous fungus fermentation liquor, and preparing the mixed liquor into lyophilized powder according to the conventional method in the field; or only uniformly mixing microbial fermentation liquor and excipient liquor; or only uniformly mixing the bacillus fermentation liquor and the filamentous fungus fermentation liquor. The microbial deodorant is simple to prepare and can be used for quickly removing peculiar smells and simultaneously removing smelly-source substances completely.
Description
Technical field
The invention belongs to the microbial environment protecting field, be specifically related to a kind of microbial deodorant and its production and application.
Background technology
Distribute in airborne stench and mainly be foul smell, be foul smell and be that foul smell is formed with the sulfur of mercaptan representative with the alkali of ammonia representative by acid with lower fatty acid representative.Removing malodorous conventional method at present mainly contains: chemical method, physics method and bioanalysis etc.Chemical method alleviates stench by using deodorizer and aromatic, and wherein smelly source material can not be thoroughly removed in the use of chemical deodorizing agent, and deodorizing effect is insufficient not thorough, and the aromatic of Shi Yonging is crossed by force and then can be brought unpleasant odour because of covering spice sometimes simultaneously.The physics method absorbs stink substance in the micropore of absorbent by using absorbent, but absorbent just is difficult to desorbing after in a single day absorbing stink substance, can't reuse to be difficult to handle, and causes second environmental pollution.The bioanalysis mainly bio-metabolic process by microorganism and relevant enzyme is used for the smelly source of digest and decompose material, this method service condition gentleness, and it is thorough to eliminate smelly source material, and can not cause second environmental pollution.In addition, prior art also has research to remove stench by the smelly source material that burns, but the smelly source material that burns can produce large quantity of exhaust gas and odor pollution environment, also has the development limitation.
Patent of invention liquid deodorant of the prior art, inventor mountain pass Ji Zi etc., application number is 00805018.X, this patent is mainly by suitable buffer agent and the surfactant of optimum ratio and add an amount of essence and remove stink.Patent of invention exhaust air deodorization device and method, inventor Zheng Shi controls etc., and application number is 200710129839.5, and this patent reaches the effect of deodorization dehumidifying by zeolite layer absorption waste gas.Patent of invention contains as the deodorizer of the dry cell of microorganism of active component and deodorizing method, old pool, the artificial sea of invention is true etc., application number is 200510003675.2, the antibacterial of the preferred Escherichia of this patent, brevibacterium and bacillus, make dry cell of microorganism deodorizer by fermentation, heat sterilization, though effect is better than active carbon, but this method is used the somatic cells adsorption of deactivation, can not utilize microbial activity enzyme decomposition smell chemical compound, from basic solution bad-smell problem, odor removal efficient is not unsatisfactory.
Summary of the invention
Technical problem to be solved by this invention is to have overcome the insufficient unpleasant odour that thoroughly easily do not produce of chemical method deodorizing effect in the existing deodorizer, the physics method easily causes secondary pollution by using absorbent, or bioanalysis can't fundamentally solve bad-smell problem and the unsatisfactory defective of odor removal efficient, provides a kind of abnormal flavour of removing fast thoroughly to eliminate microbial deodorant of smelly source material and its production and application simultaneously.
Microbial deodorant of the present invention, it contains bacillus and filamentous fungi Fungus.
Among the present invention, that described bacillus cereus is preferable is Bacillus licheniformis Bacillus licheniformis and/or Bacillus coagulans Bacillus coagulans.
When bacillus cereus contains Bacillus licheniformis Bacillus licheniformis and its deodorizing effect of Bacillus coagulans Bacillus coagulans is better simultaneously.
Wherein, described Bacillus licheniformis belongs to the bacteria culture of Bacillus, that preferable is Bacillus licheniformis Bacillus licheniformis, one or more that better is among Bacillus licheniformis Bacillus licheniformisCGMCC 1.265, Bacillus licheniformis Bacillus licheniformis CGMCC 1.1858 and the Bacillus licheniformis Bacillus licheniformis CGMCC 1.518.Described Bacillus licheniformis content preferable for the bacterium number accounts for the percentage ratio 30%～50% of microbial deodorant total bacteria count, better is 40%.Bacillus licheniformis can effectively be decomposed various carbohydrates, sugar and range protein aminoacid, for other effective microbes provide propagation required nutrient substance, reduces to produce malodorous compounds.
Wherein, described Bacillus coagulans belongs to the bacteria culture of Bacillus, that preferable is Bacillus coagulans Bacillus coagulans, one or more that better is among Bacillus coagulans Bacillus coagulansCGMCC 1.949, Bacillus coagulans Bacillus coagulans CGMCC 1.2009, Bacillus coagulans Bacillus coagulans ATCC 10545, Bacillus coagulans Bacillus coagulans ATCC21366 and the Bacillus coagulans Bacillus coagulans ATCC 31284.The content of described Bacillus coagulans preferable for the bacterium number accounts for the percentage ratio 30%～50% of microbial deodorant total bacteria count, better is 40%.Bacillus coagulans can effectively be decomposed various carbohydrates, sugar and range protein aminoacid and micromolecular compound, reduces to produce malodorous compounds, eliminates stink.
When bacillus cereus only contains Bacillus licheniformis Bacillus licheniformis and Bacillus coagulans Bacillus coagulans the two any, preferred concrete strain class as previously mentioned, simultaneously described bacillus cereus content preferable for the bacterium number accounts for the percentage ratio 60%～80% of microbial deodorant total bacteria count, better is 80%.
Among the present invention, what described filamentous fungi was preferable is monascus ruber.Described monascus ruber belongs to the strain of aspergillus fungi, that preferable is red monascus Monascus ruber, one or more that better is among red monascus Monascus ruber CGMCC 3.549, red monascus Monascus ruber CGMCC3.4636 and the red monascus Monascus ruber CGMCC 3.4701.The content of described red monascus preferable for the bacterium number accounts for the percentage ratio 20%～40% of microbial deodorant total bacteria count, better is 20%.Red monascus can be secreted multiple enzyme, and as protease, peptidase, lipase and other hydrolase etc., effectively decomposing organic matter and generation odor compound are eliminated abnormal flavour.
There is good synergism between the microorganism that microbial deodorant of the present invention contains.Under this synergism, effectively decompose a large amount of spoilage organism matter, suppress the harmful microorganism growth, eliminate stink.Wherein, filamentous fungi can effectively be decomposed various carbohydrates, sugar, starch and range protein aminoacid, for other effective microbes provide propagation required nutrient substance; Secreted multiple enzyme as cellulase, hemicellulase, xylanase, amylase, pectase and ligninase etc., can more effectively decompose the organic substance that produces stink.Secondary metabolite antibiotic and antibacterial peptide that bacillus cereus produced, can effectively suppress the harmful microbe breeding, kill deleterious pathogen to people and animals, its secreted multiple enzyme of while, can cut grease effectively and the protein refuse as protease, peptidase, lipase, amylase and saccharase etc., eliminate abnormal flavour.
Strain of the present invention all can be bought from China Committee for Culture Collection of Microorganisms common micro-organisms center (CGMCC) and American Type Culture Collecti (ATCC).
What the existence form of microbial deodorant of the present invention was preferable is the mixture of lyophilized powder, microbial inoculum, microbial inoculum and figuration liquid or the mixture of microbial inoculum and solid adjuvant material.
Wherein, described figuration liquid can be the conventional figuration liquid that uses in this area, the preferable glycerol 10% that contains, and water 80%, carbohydrate 5%, trace element 4% and spice 1%, percentage ratio account for the mass percent of figuration liquid total amount for each composition.What wherein, described carbohydrate was preferable is glucose and/or sodium acetate.Described trace element preferable for containing mass percent ferrous sulfate 0.4%, the aqueous solution of magnesium sulfate 0.5% and potassium chloride 1%.Described spice can be the conventional spice that uses in this area.
Wherein, described solid adjuvant material is the conventional solid adjuvant material in this area, and described solid adjuvant material is preferable contains milk powder and calcium carbonate; What wherein, the consumption of described milk powder was preferable is 10% of microbial inoculum percent by volume; What the consumption of described calcium carbonate was preferable is 50% of microbial inoculum quality percent by volume.
The invention still further relates to the preparation method of microbial deodorant of the present invention:
When the existence form of described microbial deodorant was lyophilized powder, preparation method comprised behind fermentation of bacillus liquid and the filamentous fungi fermentation liquid uniform mixing, makes lyophilized powder by this area conventional method and get final product; After perhaps fermentation of bacillus liquid and filamentous fungi fermentation liquid being made lyophilized powder by this area conventional method respectively, uniform mixing; Perhaps bacillus cereus lyophilized powder and filamentous fungi lyophilized powder uniform mixing get final product;
When the existence form of described microbial deodorant was microbial inoculum, preparation method comprised fermentation of bacillus liquid and filamentous fungi fermentation liquid uniform mixing, gets final product;
When the existence form of described microbial deodorant is the mixture of microbial inoculum and figuration liquid, preparation method comprises after fermentation of bacillus liquid and the mixing of filamentous fungi fermentation liquid, again with figuration liquid according to bacterium liquid: figuration liquid volume ratio is 1: 10～1: 50 uniform mixing, gets final product;
When described microbial deodorant existence form was the mixture of microbial inoculum and solid adjuvant material, preparation method comprised fermentation of bacillus liquid and filamentous fungi fermentation liquid and solid adjuvant material uniform mixing, 28 ℃～40 ℃ oven dry, and pulverizing gets final product.
Wherein, described fermentation of bacillus liquid and filamentous fungi fermentation liquid all obtain according to the cultivation program of this area routine, all cultivate and fermentation culture through slant culture, first order seed.
Wherein, the slant medium that bacillus cereus relates to, first order seed culture medium and fermentation medium preferable for nutrient broth medium, specifically filling a prescription preferable is: peptone 0.3%～2%, Carnis Bovis seu Bubali cream 0.3%～2%, sodium chloride 0.3%～1%, pH are 7.0; Wherein, slant medium also contains 1.4%～2% agar powder, and above-mentioned percentage ratio is mass percent.Wherein, what the cultivation temperature that first order seed is cultivated was preferable is 30 ℃～37 ℃, and what the first order seed incubation time was preferable is 1～2 day; What the cultivation temperature of fermentation culture was preferable is 30 ℃～37 ℃, and what fermented incubation time was preferable is 2～4 days, and what the inoculum concentration of fermentation culture was preferable is 5%～15%.
Wherein, the slant medium that filamentous fungi relates to, first order seed culture medium and fermentation medium preferable for improved PDA culture medium, specifically filling a prescription preferable is: Rhizoma Solani tuber osi 30%～40%, glucose 2%～3%, Fructus Hordei Germinatus extract 0.2%～0.5%, pH are 6.5～7.0; Wherein, slant medium also contains 1.4%～2.0% agar powder, and above-mentioned percentage ratio is mass percent.Wherein, what the cultivation temperature that first order seed is cultivated was preferable is 25 ℃～28 ℃, and what the first order seed incubation time was preferable is 4～6 days; What the cultivation temperature of fermentation culture was preferable is 25 ℃～28 ℃, and what fermented incubation time was preferable is 4～7 days, and what the inoculum concentration of fermentation culture was preferable is 5%～15%.
The invention still further relates to the application of microbial deodorant of the present invention in disposal of refuse deodorization or deodorizing waste water.
Microbial deodorant of the present invention directly is sprayed on the abnormal flavour that stink substance just can effectively be removed ammonia, trimethylamine, lower fatty acid etc., more can thoroughly eliminate smelly source material.
Agents useful for same of the present invention and raw material except that specified otherwise, all commercially available getting.
On the basis that meets this area general knowledge, each above-mentioned among the present invention technical characterictic can combination in any obtain preferred embodiments.
Positive progressive effect of the present invention is: the invention provides a kind of microbial deodorant and its production and application.This microbial deodorant can be removed abnormal flavour fast and thoroughly eliminate smelly source material simultaneously, keeps the deodorization effect for a long time.
The specific embodiment
Mode below by embodiment further specifies the present invention, but does not therefore limit the present invention among the described scope of embodiments.
Among the following embodiment, except that specified otherwise, percentage ratio is mass percent.Described trace element is the aqueous solution that contains ferrous sulfate 0.4%, magnesium sulfate 0.5% and potassium chloride 1%.
Embodiment 1
The preparation of microbial deodorant:
Bacillus licheniformis Bacillus licheniformis CGMCC 1.265, Bacillus coagulans Bacillus coagulans CGMCC 1.2009 and monascus Monascus ruber CGMCC 3.549 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 50%, Bacillus coagulans 30%, monascus 20%; Then with bacterium liquid and figuration liquid liquid according to 1: 10 uniform mixing of volume ratio, get final product.
Wherein, the prescription of figuration liquid is a glycerol 10%, water 80%, carbohydrates glucose 5%, trace element 4%, spice 1%.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 0.3%, and Carnis Bovis seu Bubali cream 2%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 2% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 37 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 37 ℃, and fermentation culture 4 days, the inoculum concentration of fermentation culture are 5%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 40%, and glucose 2%, Fructus Hordei Germinatus extract 0.5%, pH are 6.5; Wherein, slant medium also contains 2.0% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 25 ℃, and the first order seed incubation time is 4 days; The cultivation temperature of fermentation culture is 25 ℃, and fermented incubation time is 7 days, and the inoculum concentration of fermentation culture is 15%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 2
The preparation of microbial deodorant:
Bacillus licheniformis Bacillus licheniformis CGMCC 1.1858, Bacillus coagulans Bacillus coagulans CGMCC 1.949 and monascus Monascus ruber CGMCC 3.4636 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 30%, Bacillus coagulans 30%, monascus 40%; Then with bacterium liquid and figuration liquid liquid according to 1: 50 uniform mixing of volume ratio, get final product.
Wherein, the prescription of figuration liquid is a glycerol 10%, water 80%, carbohydrate sodium acetate 5%, trace element 4%, spice 1%.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 2%, and Carnis Bovis seu Bubali cream 0.3%, sodium chloride 0.3%, pH are 7.0; Wherein, slant medium also contains 1.4% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 30 ℃, and the first order seed incubation time is 1 day, and the cultivation temperature of fermentation culture is 30 ℃, and fermentation culture 4 days, the inoculum concentration of fermentation culture are 10%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 30%, and glucose 3%, Fructus Hordei Germinatus extract 0.2%, pH are 6.5; Wherein, slant medium also contains 1.4% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 28 ℃, and the first order seed incubation time is 5 days; The cultivation temperature of fermentation culture is 28 ℃, and fermented incubation time is 5 days, and the inoculum concentration of fermentation culture is 5%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 3
The preparation of microbial deodorant:
Bacillus licheniformis Bacillus licheniformis CGMCC 1.518, Bacillus coagulans Bacillus coagulans ATCC 10545 and monascus Monascus ruber CGMCC 3.4701 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 30%, Bacillus coagulans 30%, monascus 40%; Get final product.During use, 100 times of the direct thin ups of mixed bacteria liquid can be got final product.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 1%, and Carnis Bovis seu Bubali cream 1%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 1.8% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 34 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 34 ℃, and fermentation culture 3 days, the inoculum concentration of fermentation culture are 10%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 30%, and glucose 3%, Fructus Hordei Germinatus extract 0.2%, pH are 6.5; Wherein, slant medium also contains 1.4% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 28 ℃, and the first order seed incubation time is 6 days; The cultivation temperature of fermentation culture is 28 ℃, and fermented incubation time is 4 days, and the inoculum concentration of fermentation culture is 5%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 4
The preparation of microbial deodorant (solid preparation):
Bacillus licheniformis Bacillus licheniformis CGMCC 1.518, Bacillus coagulans Bacillus coagulans ATCC 31284 and monascus Monascus rubber CGMCC 3.4701 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 30%, Bacillus coagulans 30%, monascus 40%; Then microbial inoculum is added 10% (percent by volume) milk powder and 50% (quality percent by volume) calcium carbonate, mixing, oven dry below 40 ℃, pulverizing gets final product.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 1%, and Carnis Bovis seu Bubali cream 1%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 1.8% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 34 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 34 ℃, and fermentation culture 3 days, the inoculum concentration of fermentation culture are 10%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 30%, and glucose 3%, Fructus Hordei Germinatus extract 0.2%, pH are 6.5; Wherein, slant medium also contains 1.4% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 28 ℃, and the first order seed incubation time is 6 days; The cultivation temperature of fermentation culture is 28 ℃, and fermented incubation time is 4 days, and the inoculum concentration of fermentation culture is 5%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 5
The preparation of microbial deodorant:
Bacillus licheniformis Bacillus licheniformis CGMCC 1.265, Bacillus coagulans Bacillus coagulans ATCC 21366 and monascus Monascus ruber CGMCC 3.549 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 30%, Bacillus coagulans 50%, monascus 20%; Then with bacterium liquid and figuration liquid liquid according to 1: 30 uniform mixing of volume ratio, get final product.
Wherein, the prescription of figuration liquid is a glycerol 10%, water 80%, carbohydrates glucose 5%, trace element 4%, spice 1%.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 0.3%, and Carnis Bovis seu Bubali cream 2%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 2% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 37 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 37 ℃, and fermentation culture 2 days, the inoculum concentration of fermentation culture are 15%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 40%, and glucose 2%, Fructus Hordei Germinatus extract 0.5%, pH are 7.0; Wherein, slant medium also contains 2.0% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 25 ℃, and the first order seed incubation time is 4 days; The cultivation temperature of fermentation culture is 25 ℃, and fermented incubation time is 7 days, and the inoculum concentration of fermentation culture is 15%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 6
The preparation of microbial deodorant:
With Bacillus licheniformis Bacillus licheniformis CGMCC 1.265 and the Bacillus licheniformis Bacillus licheniformis CGMCC 1.518 of bacterium number than 1: 1, Bacillus coagulans Bacillus coagulans CGMCC 1.2009 and the Bacillus coagulans Bacillus coagulansATCC 10545 of bacterium number than 1: 1, and the bacterium number than 1: 1 red monascus Monascus ruber CGMCC 3.4636 and the fermentation liquid of monascus Monascus ruber CGMCC 3.549 make lyophilized powder respectively, the percentage ratio that accounts for total bacteria count according to the bacterium numbers of following various bacterium mixes: Bacillus licheniformis 40%, Bacillus coagulans 40%, monascus 20% gets final product.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 0.3%, and Carnis Bovis seu Bubali cream 2%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 2% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 37 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 37 ℃, and fermentation culture 4 days, the inoculum concentration of fermentation culture are 5%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 40%, and glucose 2%, Fructus Hordei Germinatus extract 0.5%, pH are 6.5; Wherein, slant medium also contains 2.0% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 25 ℃, and the first order seed incubation time is 4 days; The cultivation temperature of fermentation culture is 25 ℃, and fermented incubation time is 7 days, and the inoculum concentration of fermentation culture is 15%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 7
The preparation of microbial deodorant:
Bacillus licheniformis Bacillus licheniformis CGMCC 1.265 and monascus Monascusruber CGMCC 3.549 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus licheniformis 80% monascus 20%; Making lyophilized powder then gets final product;
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 0.3%, and Carnis Bovis seu Bubali cream 2%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 2% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 37 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 37 ℃, and fermentation culture 4 days, the inoculum concentration of fermentation culture are 5%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 40%, and glucose 2%, Fructus Hordei Germinatus extract 0.5%, pH are 6.5; Wherein, slant medium also contains 2.0% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 25 ℃, and the first order seed incubation time is 4 days; The cultivation temperature of fermentation culture is 25 ℃, and fermented incubation time is 7 days, and the inoculum concentration of fermentation culture is 15%.Count plate afterwards detects the bacterium number of filamentous fungi.
Embodiment 8
The preparation of microbial deodorant:
Bacillus coagulans Bacillus coagulans CGMCC 1.2009 and monascus Monascusruber CGMCC 3.549 are mixed according to the percentage ratio that the bacterium numbers of following various bacterium accounts for total bacteria count: Bacillus coagulans 60%, monascus 40%; After making lyophilized powder respectively, uniform mixing gets final product.
Wherein, the employed slant medium of bacillus cereus, first order seed culture medium and fermentation culture are nutrient broth medium, concrete prescription: peptone 0.3%, and Carnis Bovis seu Bubali cream 2%, sodium chloride 1%, pH are 7.0; Wherein, slant medium also contains 2% agar powder, and wherein, the cultivation temperature that first order seed is cultivated is 37 ℃, and the first order seed incubation time is 2 days, and the cultivation temperature of fermentation culture is 37 ℃, and fermentation culture 4 days, the inoculum concentration of fermentation culture are 5%.Count plate afterwards detects the bacterium number of bacillus cereus.
Wherein, the employed slant medium of filamentous fungi, first order seed culture medium and fermentation medium are improved PDA culture medium, concrete prescription: Rhizoma Solani tuber osi 40%, and glucose 2%, Fructus Hordei Germinatus extract 0.5%, pH are 6.5; Wherein, slant medium also contains 2.0% agar powder.Wherein, first order seed is cultivated and the cultivation temperature of fermentation culture is 25 ℃, and the first order seed incubation time is 4 days; The cultivation temperature of fermentation culture is 25 ℃, and fermented incubation time is 7 days, and the inoculum concentration of fermentation culture is 15%.Count plate afterwards detects the bacterium number of filamentous fungi.
Effect embodiment 1
With Bacillus licheniformis Bacillus licheniformis CGMCC 1.265, Bacillus coagulans Bacillus coagulans CGMCC 1.2009 and monascus Monascus ruber CGMCC 3.549 with the method for embodiment 1 cultivate zymocyte liquid, respectively get then with three kinds of bacterium liquid that embodiment 1 deodorizer bacterium is counted same amount and must can prepare BLC (Bacillus licheniformis), BCC (Bacillus coagulans), three kinds of single bacterium deodorizer of MRC (monascus) with the consistent mixed liquor of embodiment 1 volume with figuration liquid uniform mixing respectively.
The microbial deodorant that embodiments of the invention 1 are made, be made into the dilution bacterium liquid (using 10 times of deionized water dilutions) of 1/10th concentration respectively with BLC, BCC, the single bacterium deodorizer of MRC, mixing 10 minutes, deodorization bacterium liquid; 250 kilograms of rotten rubbish of sampling in the burning city domestic garbage factory trash repository fully mix the back and respectively get 50 kilograms with " five point-scores " at random, add 1#, 2#, 3#, 4#, 5# proof box (1 cubic metre) simultaneously, add a cover placement 3 days.
This test mainly detects ammonia, hydrogen sulfide, three indexs of odor concentration with reference to national odorant pollutant discharge standard GB 14554-93; After three days, detect initial value; Then 200mL clear water (1# case) and 100mL bacterium liquid (2#, 3#, 4#, 5# case) are evenly sprayed in the rubbish surface, took a sample respectively, detect in 10 minutes, 4 hours after the hydrojet from charge door.
Table 1 result of the test
By above-mentioned experiment as can be known, the deodorizer of the embodiment of the invention 1 is for rotten rubbish deodorization instant effect, and effect is obvious, persistent period reached more than 4 hours, and under equal consumption situation, its deodorizing effect is far above other three kinds single bacterium deodorizer, the synergism maximum, effect is best.
Effect embodiment 2
Implement on-the-spot deodorizing test in refuse landfill, meteorological condition be temperature about 25 ℃, wind speed is below 3 grades.Establish sampled point of laying at the center, operating area, test item: ammonia, two projects of hydrogen sulfide, background values detects before spraying; Consumption sprays 100mL 1/10th concentration deodorizer by one square metre to carry out; Detected in 10 minutes, 40 minutes behind the deodorizer of sprinkling embodiment 2 preparations.
Table 2 result of the test
Deodorizer is for household refuse landfill sites deodorization instant effect, and effect is obvious.
Effect embodiment 3
Hydrogen sulfide and ammonia are that topmost foul smell is formed in urban sewage treatment system.Instrument and method: methylene-blue colorimetric method is adopted in the detection of hydrogen sulfide, and ammonia adopts hypochlorous acid to receive-the salicylic acid spectrophotography.Sampled point is apart from the water surface 10～50cm, with 1L/min traffic sampling 20min.Instrument: Q-2C type atmosphere sampling instrument, ultra-violet and visible spectrophotometer.
Hydrogen sulfide in the static cesspool: mean concentration is 2.54mg/m
3, 32 ℃ of temperature degree, add the microbial deodorant of 10PPM embodiment 3 preparation after, after cure hydrogen mean concentration was 1.63mg/m in 10 minutes
3, after 30 minutes, concentration of hydrogen sulfide is 0.72mg/m
3, be 0.68mg/m after 4 hours
3, 1.3mg/m after 8 hours
3
Table 3 Sewage Plant deodorizing effect
| Sequence number | Add the bacterium time | Hydrogen sulfide mean concentration mg/m 3 | Clearance | Temperature ℃ |
| 1 | Former water | 2.54 | / | 32 |
| 2 | 10 minutes | 1.63 | 35.8％ | 32 |
| 3 | 30 minutes | 0.72 | 71.6％ | 32 |
| 4 | 4 hours | 0.68 | 73.2％ | 34 |
| 5 | 6 hours | 0.75 | 70.4％ | 33 |
| 6 | 8 hours | 1.3 | 48.8％ | 30 |
The result shows that the microbial deodorant zymocyte liquid of embodiment 3 preparations is better for hydrogen sulfide removal effect in municipal sewage plant's sewage, and the persistent period reached more than 4 hours.
Claims (10)
1. microbial deodorant, it is characterized in that: it contains bacillus and filamentous fungi Fungus.
2. microbial deodorant as claimed in claim 1 is characterized in that: described bacillus cereus is Bacillus licheniformis Bacillus licheniformis and/or Bacillus coagulans Bacillus coagulans; Described filamentous fungi is monascus ruber Monascus ruber.
3. microbial deodorant as claimed in claim 2 is characterized in that: described Bacillus licheniformis is one or more among Bacillus licheniformis Bacillus licheniformis CGMCC 1.265, Bacillus licheniformis Bacillus licheniformis CGMCC 1.1858 and the Bacillus licheniformis Bacillus licheniformisCGMCC 1.518; Described Bacillus coagulans is one or more among Bacillus coagulans Bacillus coagulans CGMCC 1.949, Bacillus coagulans Bacillus coagulans CGMCC1.2009, Bacillus coagulans Bacillus coagulans ATCC 10545, Bacillus coagulans Bacilluscoagulans ATCC 21366 and the Bacillus coagulans Bacillus coagulans ATCC 31284; Described monascus ruber is one or more among red monascus Monascus ruber CGMCC 3.549, red monascus Monascus ruber CGMCC 3.4636 and the red monascus Monascus ruberCGMCC 3.4701.
4. as each described microbial deodorant of claim 2～3, it is characterized in that: when bacillus cereus contains Bacillus licheniformis Bacillus licheniformis and Bacillus coagulans Bacilluscoagulans simultaneously, described Bacillus licheniformis content accounts for the percentage ratio 30%～50% of microbial deodorant total bacteria count for the bacterium number, and better is 40%; The content of described Bacillus coagulans accounts for the percentage ratio 30%～50% of microbial deodorant total bacteria count for the bacterium number, and better is 40%; The content of described red monascus accounts for the percentage ratio 20%～40% of microbial deodorant total bacteria count for the bacterium number, and better is 20%; When bacillus cereus only contains Bacillus licheniformis Bacillus licheniformis and Bacillus coagulans Bacilluscoagulans the two any, described bacillus cereus content accounts for the percentage ratio 60%～80% of microbial deodorant total bacteria count for the bacterium number, and better is 80%; The content of described red monascus accounts for the percentage ratio 20%～40% of microbial deodorant total bacteria count for the bacterium number, and better is 20%.
5. as each described microbial deodorant of claim 1～4, it is characterized in that: the existence form of described microbial deodorant is the mixture of lyophilized powder, microbial inoculum, microbial inoculum and figuration liquid or the mixture of microbial inoculum and solid adjuvant material;
Described figuration liquid contains glycerol 10%, water 80%, and carbohydrate 5%, trace element 4% and spice 1%, percentage ratio account for the mass percent of figuration liquid total amount for each composition; Described carbohydrate is glucose and/or sodium acetate; Described trace element is the ferrous sulfate 0.4% that contains mass percent, the aqueous solution of magnesium sulfate 0.5% and potassium chloride 1%;
Described solid adjuvant material contains milk powder and calcium carbonate; Wherein, the consumption of described milk powder is 10% of a microbial inoculum percent by volume; The consumption of described calcium carbonate is 50% of a microbial inoculum quality percent by volume.
6. the preparation method of a microbial deodorant is characterized in that:
When the existence form of described microbial deodorant was lyophilized powder, preparation method comprised behind fermentation of bacillus liquid and the filamentous fungi fermentation liquid uniform mixing, makes lyophilized powder by this area conventional method and get final product; After perhaps fermentation of bacillus liquid and filamentous fungi fermentation liquid being made lyophilized powder by this area conventional method respectively, uniform mixing; Perhaps bacillus cereus lyophilized powder and filamentous fungi lyophilized powder uniform mixing get final product; When the existence form of described microbial deodorant was microbial inoculum, preparation method comprised fermentation of bacillus liquid and filamentous fungi fermentation liquid uniform mixing, gets final product;
When the existence form of described microbial deodorant is the mixture of microbial inoculum and figuration liquid, preparation method comprises after fermentation of bacillus liquid and the mixing of filamentous fungi fermentation liquid, again with figuration liquid according to bacterium liquid: figuration liquid volume ratio is 1: 10～1: 50 uniform mixing, gets final product;
When described microbial deodorant existence form was the mixture of microbial inoculum and solid adjuvant material, preparation method comprised fermentation of bacillus liquid and filamentous fungi fermentation liquid and solid adjuvant material uniform mixing, 28 ℃～40 ℃ oven dry, and pulverizing gets final product.
7. preparation method as claimed in claim 6 is characterized in that: described fermentation of bacillus liquid and filamentous fungi fermentation liquid are cultivated and fermentation culture through slant culture, first order seed.
8. preparation method as claimed in claim 7, it is characterized in that: the slant medium that described bacillus cereus relates to, first order seed culture medium and fermentation medium are nutrient broth medium, concrete prescription is: peptone 0.3%～2%, Carnis Bovis seu Bubali cream 0.3%～2%, sodium chloride 0.3%～1%, pH are 7.0; Wherein, slant medium also contains 1.4%～2% agar powder, and above-mentioned percentage ratio is mass percent;
The slant medium that described filamentous fungi relates to, first order seed culture medium and fermentation medium are improved PDA culture medium, and concrete prescription is: Rhizoma Solani tuber osi 30%～40%, and glucose 2%～3%, Fructus Hordei Germinatus extract 0.2%～0.5%, pH are 6.5～7.0; Wherein, slant medium also contains 1.4%～2.0% agar powder, and above-mentioned percentage ratio is mass percent.
9. as claim 7 or 8 described preparation methoies, it is characterized in that: the cultivation temperature that described bacillus cereus first order seed is cultivated is 30 ℃～37 ℃, and the first order seed incubation time is 1～2 day; The cultivation temperature of fermentation culture is 30 ℃～37 ℃, and fermented incubation time is 2～4 days, and the inoculum concentration of fermentation culture is 5%～15%; The cultivation temperature that described filamentous fungi first order seed is cultivated is 25 ℃～28 ℃, and the first order seed incubation time is 4～6 days; The cultivation temperature of fermentation culture is 25 ℃～28 ℃, and fermented incubation time is 4～7 days, and the inoculum concentration of fermentation culture is 5%～15%.
10. one kind as each described microbial deodorant application in disposal of refuse deodorization or deodorizing waste water of claim 1～5.
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