CN102688516B - Deodorizing and dedusting agent for chicken farm and preparation method thereof - Google Patents
Deodorizing and dedusting agent for chicken farm and preparation method thereof Download PDFInfo
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- CN102688516B CN102688516B CN 201110392111 CN201110392111A CN102688516B CN 102688516 B CN102688516 B CN 102688516B CN 201110392111 CN201110392111 CN 201110392111 CN 201110392111 A CN201110392111 A CN 201110392111A CN 102688516 B CN102688516 B CN 102688516B
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Abstract
The invention discloses a deodorizing and dedusting agent for a chicken farm, comprising the following components in parts by weight: 30-50 parts of bacillus subtilis fermentation liquor, 5-10 parts of soya bean lecithin and 1-2 parts of plant essential oil; and the mixed fermentation liquor is formed by fermenting bacillus subtilis liquor with the viable bacterium amount over 108/mL and enterococcus faecalis liquor which are in the weight ratio of 1:(1-5). The deodorizing and dedusting agent for the chicken farm, provided by the invention, is made of pure natural preparations so as to have no toxicity or harm to working staffs and the chicken crowd and have a health care function. The deodorizing and dedusting agent has the application amount of 5-10mL/m<3> when being used.
Description
Technical field
The present invention relates to a kind of chicken farm deodorizer used, for eliminating the deodorization dedusting agent of hen house or hatchery foul odour and feather dirt, relate to a kind of preparation method of this deodorization dedusting agent exactly simultaneously.
Background technology
Chicken individuals is little, grows soon, and health, skin and feather metabolism are vigorous.The ammonia that chicken manure just produces, hydrogen sulfide etc. cause insufferable fishy odor.Simultaneously, from hatching shell to growing up, the chicken Common Swift will experience down, juvenile plumage, young plumage and four moult of adult plumage.Especially the infancy, chicken be take down as main, in hatchery, picks up between young bird and hen house brood time, and fine hair flies upward, and causes greatly environmental pollution.Feather is divided into front wing, down and sends out plumage from structure, and small pieces front wing and a plumage and scurf etc. are also the key factors that causes large hen house floating dust.These floating dust not only endanger staff and chicken group's respiratory system, but also may carry pathogenic microorganism, spread disease.
Summary of the invention
The purpose of this invention is to provide a kind of chicken farm deodorization dedusting agent used, eliminate foul odour and feather dirt in hen house or hatchery.
In order to realize above purpose, the technical solution adopted in the present invention is: a kind of poultry place deodorization dedusting agent is comprised of the component of following weight portion: 30～50 parts of mixed culture fermentation liquid, 5～10 parts of soybean lecithins, 1～2 part of plants essential oil; Described mixed culture fermentation liquid is to be formed by the bacillus subtilis liquid more than number of viable 108/mL and the fermentation of enterococcus faecalis liquid, and the weight ratio of described bacillus subtilis liquid and enterococcus faecalis liquid is 1: 1～5.
Described fermentation of bacillus subtilis liquid is to adopt bacillus subtilis liquid to form in 30～39 ℃ of condition bottom fermentation 60～72h in culture medium.
Described Enterococcus faecalis fermentation liquid is to adopt enterococcus faecalis liquid to form in 30～39 ℃ of condition bottom fermentation 60～72h in culture medium.
Described culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water.
Chicken farm of the present invention deodorization dedusting agent used adopts following method preparation:
1) preparation of bacillus subtilis liquid: the first medium component is glucose 30g, peptone 18g, sodium chloride 8g, Carnis Bovis seu Bubali cream 1g, agar 20g, distilled water 1L, pH7.2,121 ℃ of autoclaving 20min; Getting by weight percentage 5% bacillus subtilis and 95% bacillus subtilis bacterium culture medium, under aseptic condition, bacillus subtilis is inoculated in to culture medium, is under 35～39 ℃ of conditions in temperature, cultivates 12～24 hours, forms bacillus subtilis liquid;
The preparation of enterococcus faecalis liquid: the second medium component and percentage by weight thereof are agar 15g, peptone 10g, Carnis Bovis seu Bubali cream 10g, glucose 20g, potassium dihydrogen phosphate 2g, sodium acetate 5g, ammonium citrate 2g, magnesium sulfate 0.5g, manganese sulfate 0.2g, distilled water 1L, pH6.5,121 ℃ of autoclaving 20min; Getting by weight percentage 5% enterococcus faecalis and 95% enterococcus faecalis culture medium, under aseptic condition, enterococcus faecalis is inoculated in to culture medium, is under 35～39 ℃ of conditions in temperature, cultivates 12～24 hours, makes enterococcus faecalis liquid.
Bacillus subtilis liquid and enterococcus faecalis liquid adopt the said method preparation or adopt commercial product.
2) mixed culture fermentation liquid: bacillus subtilis liquid and enterococcus faecalis liquid are mixed according to weight ratio 1: 1～5, the 3rd culture medium that adds 15～20 times of mixed liquor weight, this culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water, pH is 3.0～4.5, cultivate 60～72h under 30～39 ℃ of conditions, make mixed culture fermentation liquid.
3) mixed culture fermentation liquid, soybean lecithin and plants essential oil are mixed according to formula ratio and obtain the deodorization dedusting agent.
The fermentation of bacillus subtilis liquid added of the present invention and Enterococcus faecalis fermentation liquid can be eliminated the foul smell such as the ammonia of poultry in place, hydrogen sulfide; The soybean lecithin added has hydrophilic group, lipophilic group architectural characteristic, in absorption hen house and hatchery in air Corium Gallus domesticus consider to be worth doing and there is the material of oils and fats on the surface such as fine hair, settle down, play the effect of dedusting; The plants essential oil added is distinctive aromatic substance in the extraction plant, can further improve the abnormal smells from the patient in hen house or hatchery.Poultry of the present invention place deodorization dedusting agent, adopt pure natural preparation, all nontoxic to staff and chicken group, and health-care effect is arranged.During use, amount of application is 5～10mL/m
3
The specific embodiment
Embodiment 1
The poultry place deodorization dedusting agent of the present embodiment, be comprised of the component of following weight portion: 30 parts of mixed culture fermentation liquid, 5 parts of soybean lecithins, 1 part of plants essential oil; Described mixed culture fermentation liquid is by number of viable 10
8the bacillus subtilis liquid that/ml is above and the fermentation of enterococcus faecalis liquid form, and the weight ratio of described bacillus subtilis liquid and enterococcus faecalis liquid is 1: 1.
The poultry place deodorization dedusting agent of the present embodiment adopts following method preparation:
1) preparation of bacillus subtilis liquid: the first medium component is glucose 30g, peptone 18g, sodium chloride 8g, Carnis Bovis seu Bubali cream 1g, agar 20g, distilled water 1L, pH7.2,121 ℃ of autoclaving 20min; Getting by weight percentage 5% bacillus subtilis and 95% bacillus subtilis bacterium culture medium, under aseptic condition, bacillus subtilis is inoculated in to culture medium, is under 35 ℃ of conditions in temperature, cultivates 24 hours, forms bacillus subtilis liquid;
The preparation of enterococcus faecalis liquid: the second medium component and percentage by weight thereof are agar 15g, peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, glucose 20g, potassium dihydrogen phosphate 2g, sodium acetate 5g, ammonium citrate 2g, magnesium sulfate 0.5g, manganese sulfate 0.2g, distilled water 1L, pH6.5,121 ℃ of autoclaving 20min; Getting by weight percentage 5% enterococcus faecalis and 95% enterococcus faecalis culture medium, under aseptic condition, enterococcus faecalis is inoculated in to culture medium, is under 35 ℃ of conditions in temperature, cultivates 24 hours, makes enterococcus faecalis liquid.
2) mixed culture fermentation liquid: bacillus subtilis liquid and enterococcus faecalis liquid are mixed according to weight ratio at 1: 1, the 3rd culture medium that adds 15 times of mixed liquor weight, this culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water, pH is 3.0, cultivate 72h under 30 ℃ of conditions, make mixed culture fermentation liquid.
3) mixed culture fermentation liquid, soybean lecithin and plants essential oil are mixed according to formula ratio and obtain the deodorization dedusting agent.
Embodiment 2
The poultry place deodorization dedusting agent of the present embodiment, be comprised of the component of following weight portion: 40 parts of mixed culture fermentation liquid, 7 parts of soybean lecithins, 1.5 parts of plants essential oils; Described mixed culture fermentation liquid is by number of viable 10
8the bacillus subtilis liquid that/ml is above and the fermentation of enterococcus faecalis liquid form, and the weight ratio of described bacillus subtilis liquid and enterococcus faecalis liquid is 1: 3.
The poultry place deodorization dedusting agent of the present embodiment adopts following method preparation:
1) preparation of bacillus subtilis liquid: the first medium component is glucose 30g, peptone 18g, sodium chloride 8g, Carnis Bovis seu Bubali cream 1g, agar 20g, distilled water 1L, pH7.2,121 ℃ of autoclaving 20min; Getting by weight percentage 5% bacillus subtilis and 95% bacillus subtilis bacterium culture medium, under aseptic condition, bacillus subtilis is inoculated in to culture medium, is under 37 ℃ of conditions in temperature, cultivates 18 hours, forms bacillus subtilis liquid;
The preparation of enterococcus faecalis liquid: the second medium component and percentage by weight thereof are agar 15g, peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, glucose 20g, potassium dihydrogen phosphate 2g, sodium acetate 5g, ammonium citrate 2g, magnesium sulfate 0.5g, manganese sulfate 0.2g, distilled water 1L, pH6.5,121 ℃ of autoclaving 20min; Getting by weight percentage 5% enterococcus faecalis and 95% enterococcus faecalis culture medium, under aseptic condition, enterococcus faecalis is inoculated in to culture medium, is under 37 ℃ of conditions in temperature, cultivates 18 hours, makes enterococcus faecalis liquid.
2) mixed culture fermentation liquid: bacillus subtilis liquid and enterococcus faecalis liquid are mixed according to weight ratio at 1: 3, the 3rd culture medium that adds 18 times of mixed liquor weight, this culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water, pH is 3.8, cultivate 66h under 35 ℃ of conditions, make mixed culture fermentation liquid.
3) mixed culture fermentation liquid, soybean lecithin and plants essential oil are mixed according to formula ratio and obtain the deodorization dedusting agent.
Embodiment 3
The poultry place deodorization dedusting agent of the present embodiment, be comprised of the component of following weight portion: 50 parts of mixed culture fermentation liquid, 10 parts of soybean lecithins, 2 parts of plants essential oils; Described mixed culture fermentation liquid is by number of viable 10
8the bacillus subtilis liquid that/ml is above and the fermentation of enterococcus faecalis liquid form, and the weight ratio of described bacillus subtilis liquid and enterococcus faecalis liquid is 1: 5.
The poultry place deodorization dedusting agent of the present embodiment adopts following method preparation:
1) preparation of bacillus subtilis liquid: the first medium component is glucose 30g, peptone 18g, sodium chloride 8g, Carnis Bovis seu Bubali cream 1g, agar 20g, distilled water 1L, pH7.2,121 ℃ of autoclaving 20min; Getting by weight percentage 5% bacillus subtilis and 95% bacillus subtilis bacterium culture medium, under aseptic condition, bacillus subtilis is inoculated in to culture medium, is under 39 ℃ of conditions in temperature, cultivates 12 hours, forms bacillus subtilis liquid;
The preparation of enterococcus faecalis liquid: the second medium component and percentage by weight thereof are agar 15g, peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, glucose 20g, potassium dihydrogen phosphate 2g, sodium acetate 5g, ammonium citrate 2g, magnesium sulfate 0.5g, manganese sulfate 0.2g, distilled water 1L, pH6.5,121 ℃ of autoclaving 20min; Getting by weight percentage 5% enterococcus faecalis and 95% enterococcus faecalis culture medium, under aseptic condition, enterococcus faecalis is inoculated in to culture medium, is under 39 ℃ of conditions in temperature, cultivates 12 hours, makes enterococcus faecalis liquid.
2) mixed culture fermentation liquid: bacillus subtilis liquid and enterococcus faecalis liquid are mixed according to weight ratio at 1: 5, the 3rd culture medium that adds 20 times of mixed liquor weight, this culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water, pH is 3.8, cultivate 60h under 39 ℃ of conditions, make mixed culture fermentation liquid.
3) mixed culture fermentation liquid, soybean lecithin and plants essential oil are mixed according to formula ratio and obtain the deodorization dedusting agent.
Experimental example
Identical 4 hen houses of choice structure and facility, raise the identical AA broiler of age in days, 10000 every.Gather respectively 4 gases in house when 20 age in days, chosen distance ground 30～35cm is highly sampled, and every morning experimental period, 6:00 started to gather, and the persistent period is 30min, continuous acquisition 3 days.Ammonia is measured and is used the sampling of QT-2A type air sampler, by the nessler reagent colorimetry, is analyzed, and hydrogen sulfide is measured and used methylene-blue colorimetric method.Airborne dust adopts the middle flow airborne particulate matter collection device of KC-120H type with cutting head, gathers the following total air suspended particulates of 100 μ m, and flow-control is at 100L/min.Result of the test is shown in Table 1.
Table 1
Claims (4)
1. a poultry place deodorization dedusting agent, it is characterized in that: the component by following weight portion forms: 30～50 parts of mixed culture fermentation liquid, 5～10 parts of soybean lecithins, 1～2 part of plants essential oil; Described mixed culture fermentation liquid is to be formed by the bacillus subtilis liquid more than number of viable 108/ml and the fermentation of enterococcus faecalis liquid, and the weight ratio of described bacillus subtilis liquid and enterococcus faecalis liquid is 1: 1～5.
2. poultry according to claim 1 place deodorization dedusting agent, it is characterized in that: the fermentation condition of described mixed culture fermentation liquid is 30～39 ℃ of condition bottom fermentation 60～72h.
3. poultry according to claim 1 place deodorization dedusting agent, it is characterized in that: the culture medium adopted during fermentation is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water.
4. the preparation method of place deodorization dedusting agent of raising chickens as claimed in claim 1, it is characterized in that: its step is as follows:
1) preparation of bacillus subtilis liquid: the first medium component is glucose 30g, peptone 18g, sodium chloride 8g, Carnis Bovis seu Bubali cream 1g, agar 20g, distilled water 1L, PH7.2,121 ℃ of autoclaving 20min; Getting by weight percentage 5% bacillus subtilis and the first culture medium of 95%, under aseptic condition, bacillus subtilis is inoculated in to culture medium, is under 35～39 ℃ of conditions in temperature, cultivates 12～24 hours, forms bacillus subtilis liquid;
The preparation of enterococcus faecalis liquid: the second medium component is agar 15g, peptone 10g, Carnis Bovis seu Bubali cream 10g, glucose 20g, potassium dihydrogen phosphate 2g, sodium acetate 5g, ammonium citrate 2g, magnesium sulfate 0.5g, manganese sulfate 0.2g, distilled water 1L, PH6.5,121 ℃ of autoclaving 20min; Getting by weight percentage 5% enterococcus faecalis and the second culture medium of 95%, under aseptic condition, enterococcus faecalis is inoculated in to culture medium, is under 35～39 ℃ of conditions in temperature, cultivates 12～24 hours, makes enterococcus faecalis liquid;
2) mixed culture fermentation liquid: bacillus subtilis liquid and enterococcus faecalis liquid are mixed according to weight ratio 1: 1～5, the 3rd culture medium that adds 15～20 times of mixed liquor weight, this culture medium is comprised of the component of following weight portion: 1.0 parts, agar, 1.5 parts of Carnis Bovis seu Bubali creams, 1.0 parts of peptones, yeast soak 2.0 parts, powder, 1.5 parts of defoamer, 20 parts of sucrose, 1000 parts of distilled water, PH is 3.0～4.5, cultivate 60～72h under 30～39 ℃ of conditions, make mixed culture fermentation liquid;
3) mixed culture fermentation liquid, soybean lecithin and plants essential oil are mixed according to formula ratio and obtain the deodorization dedusting agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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