CN107041310A - A kind of tissue culture and rapid propagation method of ethnic drug fevervine - Google Patents
A kind of tissue culture and rapid propagation method of ethnic drug fevervine Download PDFInfo
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- CN107041310A CN107041310A CN201710292943.XA CN201710292943A CN107041310A CN 107041310 A CN107041310 A CN 107041310A CN 201710292943 A CN201710292943 A CN 201710292943A CN 107041310 A CN107041310 A CN 107041310A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The present invention relates to the method for Plant Tissue Breeding in agricultural biotechnologies, specifically, it is related to a kind of tissue culture and rapid propagation method of ethnic drug fevervine, including the following steps carried out successively：The explant of acquisition fevervine, explant induction, Multiplying culture, culture of rootage, transplanting；The explant inducing culture of described explant induction operation includes：MS culture mediums, 3.0~5.0mg/L KT, 0.5~1.0mg/L NAA, 25~30g/L sucrose, 3.5~4.0g/L agar, 0.5~1.0g/L activated carbons, explant inducing culture pH is 5.4~5.8, present invention selection fevervine stem-segment with node is explant, through inducing, breeding, take root, hardening and transplanting and other steps, it induces up to more than 91.5%, very high survival rate has been reached, the quick breeding by group culture system of ethnic drug fevervine has been established, it is achieved thereby that the purpose of the present invention.
Description
Technical field
The present invention relates to the method for Plant Tissue Breeding in agricultural biotechnologies, specifically, it is related to a kind of ethnic drug chicken
Swear the tissue culture and rapid propagation method of rattan.
Background technology
Ethnic drug fevervine (Paederia scandens) is Rubiaceae Paederia plant, with root or all herbal medicine, is
One of conventional Chinese herbal medicine of 24 ethnic groups such as Yi nationality, seedling, Tujia, with evacuate pathogenic wind dampness removing, promoting digestion and removing indigestion, cough-relieving, the work(such as analgesic
Effect, is usually used in rheumatalgia, traumatic injury, traumatic pain, liver and gall, stomach intestinal colic, icteric hepatitis, indigestion is small
Youngster's infantile malnutrition due to digestive disturbances or intestinalparasites, hemoptysis of pulmonary tuberculosis, bronchitis, leukopenia caused by radiotherapy, the treatment of the disease such as pesticide poisoning.
Paederia is generally weak prehensile shrub or liana, and the whole world there are about 40 kinds, and main product is regional in Tropical Asian, I
State-owned 12 kinds, be distributed mainly on it is southwestern, Central-South to South China, it is in the majority with the west and south, its like compared with warm environment, resist cold, give birth to
In hillside valley floor, small stream side and roadside or underbrush.Up to the present the research to fevervine is concentrated mainly on its active ingredient
In terms of pharmacology and clinical practice, and the research of its relevant reproduction technique is very few.Fevervine can pass through seed, cuttage, press strip etc.
Mode is bred, but there is cycle length, the shortcomings of breeding coefficient is low.Therefore, it is necessary to set up chicken by tissue culture technique
The tissue culture rapid propagation system of rattan is sweared, high quality seedling is provided for fevervine large-scale planting.The present invention selection fevervine stem-segment with node be
Explant, through inducing, breeding, take root, hardening and transplanting and other steps, establishes the quick breeding by group culture body of ethnic drug fevervine
System.The present invention has the features such as cycle is short, with low cost, breeding coefficient is high, can be directly used for factory's metaplasia of fevervine seedling
Production, for promoting the development of resources of ethnic drug fevervine to have important practical significance.
At present, the report of fevervine tissue culture technique is at home and abroad there is no, does not also there is fevervine tissue-culturing rapid propagation patent
Application.
The content of the invention
It is an object of the invention to provide a kind of tissue culture and rapid propagation method of ethnic drug fevervine, present invention selection fevervine belt segment
Stem section is explant, through inducing, breeding, take root, hardening and transplanting and other steps, and it induces up to more than 91.5%, reached
Very high survival rate, establishes the quick breeding by group culture system of ethnic drug fevervine, it is achieved thereby that the purpose of the present invention.
The technical scheme that the present invention is provided is：A kind of tissue culture and rapid propagation method of ethnic drug fevervine, including carry out successively
Following steps：The explant of acquisition fevervine, explant induction, Multiplying culture, culture of rootage, transplanting；
The explant inducing culture of described explant induction operation includes：MS culture mediums, 3.0~5.0mg/L KT,
0.5~1.0mg/LNAA, 25~30g/L sucrose, 3.5~4.0g/L agar, 0.5~1.0g/L activated carbons, explant induction training
It is 5.4~5.8 to support base pH.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, the method for described explant induction operation is：Will
2.0~2.5cm belt segment rattan is cut into after the belt segment rattan sterilization of the fevervine as explant collected and is inoculated with
Into inducing culture, being placed in 25~28 DEG C of full light cultures of progress 20~25 days can induced synthesis adventitious bud.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, the used propagation training of described Multiplying culture operation
Foster base includes：MS culture mediums, 3.0~5.0mg/L 6-BA, 0.5~1.0mg/LNAA, 0.1~0.3mg/L 2,4-D, 20~
30g/L sucrose, 3.5~4.0g/L agar, 0.1~0.5g/L activated carbons, proliferated culture medium pH are 5.4~5.8.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, described Multiplying culture operation is：It will be trained through propagation
The foster stem section for operating obtained adventitious bud to be cut into long 1.5~2.0cm is simultaneously inoculated into culture 15~20 days in proliferated culture medium
Realize the propagation of adventitious bud.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, the used training of taking root of described culture of rootage operation
Foster base includes：1/2MS culture mediums, 0.5~1.0mg/L GGR-7,0.05~0.1mg/L cycocels, 15~20g/L sucrose, 3.0
~4.0g/L agar, 0.1~0.3g/L activated carbons, root media pH are 5.4~5.8.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, the method for described culture of rootage operation is：From base
Portion cuts the adventitious bud for 2.0~3.0cm of length that Multiplying culture operation is obtained and is inoculated into root media and cultivates 20~25 days
Taking root for test tube seedling can be realized.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, the specific method of described transplanting operation is：Will be through
The good test tube seedling of the obtained root growth of culture of rootage operation cleans the training of root in the natural light lower refining seedling 1~2 day in greenhouse
It is 1 that foster base, which is transplanted in volume ratio,:Cultivation seedling produces seedling in 1 fertile soil, peat soil mixed-matrix.
In the tissue culture and rapid propagation method of above-mentioned ethnic drug fevervine, bar is cultivated in Multiplying culture operation, culture of rootage behaviour
Part is：Cultivation temperature is 25~28 DEG C, and intensity of illumination is 2500~3000lx, and light application time is 11~13 hours/day.
Beneficial effect：
The present invention establishes the quick breeding by group culture system of ethnic drug fevervine, the present invention using plant tissue culture technique
With the features such as the cycle is short, with low cost, breeding coefficient is high, the factorial praluction of fevervine seedling is can be directly used for, for promoting
The development of resources for entering ethnic drug fevervine has important practical significance.
Embodiment
With reference to embodiment, technical scheme is described in further detail, but not constituted pair
Any limitation of the present invention.
Embodiment one
(1) explant is gathered：Robust growth, the fevervine plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：First rinse 5 hours, be placed in super under running water when step (1) gathers back laboratory explant
Sterilized 10 seconds in 75% ethanol solution in net workbench, use after aseptic filter paper suck dry moisture, put after aseptic water washing 3 times
Sterilized 10 minutes in 0.1% mercuric chloride solution, after aseptic water washing 5 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 25 DEG C of full light cultures of progress 20 days can induced synthesis
Adventitious bud, inductivity is 95.8%, and pollution rate is less than 12%.Described inducing culture is：MS culture medium+5.0mg/L KT+
1.0mg/LNAA+25g/L sucrose+3.5g/L agar+0.5g/L activated carbons, pH is 5.4.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 15 days is cultivated in proliferated culture medium, growth coefficient reaches 7.0 times.Described proliferated culture medium
For：MS culture medium+5.0mg/L 6-BA+1.0mg/L NAA+0.3mg/L 2,4-D+20g/L sucrose+3.5g/L agar+0.1g/
L activated carbons, pH is 5.4.
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling for 20 days in root media, and rooting rate is 98%.Described root media is：1/
2MS culture medium+1.0mg/L GGR-7 (root-inducing powder)+0.1mg/L cycocel+15g/L sucrose+3.0g/L agar+0.1g/L activity
Charcoal, pH is 5.4.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 1 day in greenhouse, root is cleaned
Culture medium transplant in volume ratio be 1:Cultivation seedling produces seedling in 1 fertile soil, peat soil mixed-matrix, after transplanting 30 days
Survival rate 96.5%.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 25 DEG C, and intensity of illumination is 2500lx, light application time
For 11 hours/day.
Embodiment two
(1) explant is gathered：Robust growth, the fevervine plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：First rinse 6 hours, be placed in super under running water when step (1) gathers back laboratory explant
Sterilized 12 seconds in 75% ethanol solution in net workbench, use after aseptic filter paper suck dry moisture, put after aseptic water washing 4 times
Sterilized 14 minutes in 0.1% mercuric chloride solution, after aseptic water washing 6 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 26 DEG C of full light cultures of progress 23 days can induced synthesis
Adventitious bud, inductivity is 93.1%.Pollution rate is less than 10%.Described inducing culture is：MS culture medium+4.0mg/L KT+
0.8mg/LNAA+27g/L sucrose+3.8g/L agar+0.8g/L activated carbons, pH is 5.6.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 17 days is cultivated in proliferated culture medium, growth coefficient reaches 6.2 times.Described proliferated culture medium
For：MS culture medium+4.0mg/L 6-BA+0.8mg/L NAA+0.2mg/L 2,4-D+25g/L sucrose+3.7g/L agar+0.3g/
L activated carbons, pH is 5.6.
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling for 22 days in root media, and rooting rate is 94.8%.Described root media is：1/
2MS culture medium+0.8mg/L GGR-7 (root-inducing powder)+0.08mg/L cycocel+18g/L sucrose+3.5g/L agar+0.2g/L lives
Property charcoal, pH is 5.6.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 1.5 days in greenhouse, root is cleaned
It is 1 that the culture medium in portion, which is transplanted in volume ratio,:Cultivation seedling produces seedling in 1 fertile soil, peat soil mixed-matrix, transplants 30 days
Survival rate is 97% afterwards.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 27 DEG C, and intensity of illumination is 2700lx, light application time
For 12 hours/day.
Embodiment three
(1) explant is gathered：Robust growth, the fevervine plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：First rinse 7 hours, be placed in super under running water when step (1) gathers back laboratory explant
Sterilized 15 seconds in 75% ethanol solution in net workbench, use after aseptic filter paper suck dry moisture, put after aseptic water washing 5 times
Sterilized 15 minutes in 0.1% mercuric chloride solution, after aseptic water washing 7 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 28 DEG C of full light cultures of progress 25 days can induced synthesis
Adventitious bud, inductivity is 91.7%, and pollution rate is less than 8%.Described inducing culture is：MS culture medium+3.0mg/L KT+
0.5mg/LNAA+30g/L sucrose+4.0g/L agar+1.0g/L activated carbons, pH is 5.8.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 20 days is cultivated in proliferated culture medium, growth coefficient reaches 5.1 times.Described proliferated culture medium
For：MS culture medium+3.0mg/L 6-BA+0.5mg/L NAA+0.1mg/L 2,4-D+25g/L sucrose+3.8g/L agar+0.3g/
L activated carbons, pH is 5.6.
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling for 23 days in root media, and rooting rate is 92.9%.Described root media is：1/
2MS culture medium+0.5mg/L GGR-7 (root-inducing powder)+0.05mg/L cycocel+20g/L sucrose+4.0g/L agar+0.3g/L lives
Property charcoal, pH is 5.8.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 2 days in greenhouse, root is cleaned
Culture medium transplant in volume ratio be 1:Cultivation seedling produces seedling in 1 fertile soil, peat soil mixed-matrix, after transplanting 30 days
Survival rate is 95.6%.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 28 DEG C, and intensity of illumination is 3000lx, light application time
For 13 hours/day.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (8)
1. a kind of tissue culture and rapid propagation method of ethnic drug fevervine, it is characterised in that：Including the following steps carried out successively：Obtain chicken
The explant of arrow rattan, explant induction, Multiplying culture, culture of rootage, transplanting；
The explant inducing culture of described explant induction operation includes：MS culture mediums, 3.0~5.0mg/LKT, 0.5~
1.0mg/LNAA, 25~30g/L sucrose, 3.5~4.0g/L agar, 0.5~1.0g/L activated carbons, explant inducing culture pH
For 5.4~5.8.
2. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 1, it is characterised in that：Described explant is lured
The method for leading operation is：2.0~2.5cm will be cut into after the belt segment rattan sterilization of the fevervine as explant collected
Belt segment rattan and be inoculated into inducing culture, be placed in 25~28 DEG C carry out full light culture 20~25 days can induced synthesis not
Normal bud.
3. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 1, it is characterised in that：Described Multiplying culture
The used proliferated culture medium of operation includes：MS culture mediums, 3.0~5.0mg/L 6-BA, 0.5~1.0mg/LNAA, 0.1~
0.3mg/L 2,4-D, 20~30g/L sucrose, 3.5~4.0g/L agar, 0.1~0.5g/L activated carbons, proliferated culture medium pH is
5.4~5.8.
4. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 3, it is characterised in that：Described Multiplying culture
Operate and be：Through Multiplying culture obtained adventitious bud will be operated to be cut into long 1.5~2.0cm stem section and be inoculated into proliferated culture medium
The propagation of adventitious bud can be achieved for 15~20 days in culture.
5. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 1, it is characterised in that：Described culture of rootage
The used root media of operation includes：1/2MS culture mediums, 0.5~1.0mg/L GGR-7,0.05~0.1mg/L are short strong
Element, 15~20g/L sucrose, 3.0~4.0g/L agar, 0.1~0.3g/L activated carbons, root media pH are 5.4~5.8.
6. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 5, it is characterised in that：Described culture of rootage
The method of operation is：The adventitious bud for 2.0~3.0cm of length that Multiplying culture operation is obtained is cut from base portion and is inoculated into culture of rootage
Culture can realize taking root for test tube seedling in 20~25 days in base.
7. the tissue culture and rapid propagation method of ethnic drug fevervine according to claim 1, it is characterised in that：Described transplanting operation
Specific method be：The good test tube seedling of obtained root growth will be operated in the natural light lower refining seedling 1 in greenhouse through culture of rootage
~2 days, it was 1 that the culture medium of clean root, which is transplanted in volume ratio,:Cultivation seedling is produced in 1 fertile soil, peat soil mixed-matrix
Seedling.
8. according to the tissue culture and rapid propagation method of any described ethnic drug fevervines of claim 1-7, it is characterised in that：Multiplying culture
Condition of culture is in operation, culture of rootage behaviour：Cultivation temperature is 25~28 DEG C, and intensity of illumination is 2500~3000lx, illumination
Time is 11~13 hours/day.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982633A (en) * | 2022-05-26 | 2022-09-02 | 桂林理工大学 | Rapid propagation method of national medicine thymifoious euphorbia herb |
| CN115005096A (en) * | 2022-05-26 | 2022-09-06 | 桂林医学院 | Ethnic medicine alangium platanifolium seedling breeding method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284624A (en) * | 2015-11-16 | 2016-02-03 | 广西科技大学鹿山学院 | Rapid breeding method for stem-segment-formed seedling of oldenlandia corymbosa L. |
-
2017
- 2017-04-28 CN CN201710292943.XA patent/CN107041310B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284624A (en) * | 2015-11-16 | 2016-02-03 | 广西科技大学鹿山学院 | Rapid breeding method for stem-segment-formed seedling of oldenlandia corymbosa L. |
Non-Patent Citations (2)
| Title |
|---|
| 李任珠等: ""海南中草药材鸡屎藤离体培养的研究"", 《海南大学学报自然科学版》 * |
| 赵俊等: ""鸡屎藤愈伤组织诱导及细胞悬浮培养研究"", 《南方农业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114982633A (en) * | 2022-05-26 | 2022-09-02 | 桂林理工大学 | Rapid propagation method of national medicine thymifoious euphorbia herb |
| CN115005096A (en) * | 2022-05-26 | 2022-09-06 | 桂林医学院 | Ethnic medicine alangium platanifolium seedling breeding method |
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