CN104307012B - A kind of special deodorant of livestock and poultry farm and its application - Google Patents
A kind of special deodorant of livestock and poultry farm and its application Download PDFInfo
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- CN104307012B CN104307012B CN201410615491.0A CN201410615491A CN104307012B CN 104307012 B CN104307012 B CN 104307012B CN 201410615491 A CN201410615491 A CN 201410615491A CN 104307012 B CN104307012 B CN 104307012B
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Abstract
The invention discloses a kind of special deodorant of livestock and poultry farm and its application, its strain composition is：Lactobacillus acidophilus 106~108Individual/ml, lactobacillus reuteri 107~109Individual/ml, bacillus polymyxa 104~106Individual/ml, bacillus lentus 106~108Individual/ml, bacillus coagulans 106~109Individual/ml, enterococcus faecalis 104~106Individual/ml, VREF 107~109Individual/ml, fold candida bacterium 104~107Individual/ml, Candida valida bacterium 104~107Individual/ml, Co-158 strain 104~106Individual/ml, Rhodopseudomonas palustris 106~108Individual/ml, Geotrichum Suaveolens 104~106Individual/ml, rod inulinase 106~108Individual/ml, rough spore inulinase 106~108Individual/ml and styreptomyces globispotus strain 106~109Individual/ml.The present invention handles the burst size of the excreta of poultry house and heap fermentation, effectively reduction foul gas with spray pattern, and technical support is provided for the effective processing and environmental protection of livestock and poultry farm stench.In addition the present invention can also be sprayed onto livestock and poultry body surface, keep livestock and poultry body surface sanitation and hygiene, be conducive to the growth of livestock and poultry.
Description
Technical field
The present invention relates to a kind of deodorant, a kind of special deodorant of livestock and poultry farm and its application are specifically related to, is belonged to
Environmental protection technical field.
Background technology
With the large-scale development of livestock and poultry breeding industry, pollution of the farm animal excrement to plant's surrounding environment is also increasingly tight
Weight, wherein it is one of major way to produce foul gas.The main cause for causing feces of livestock and poultry foul gas to produce is excrement heap
The volatilization and release of the material such as ammonia, hydrogen sulfide, methane and organic acid during product.Foul gas is not only to plant's environment week
Enclose resident and bring discomfort in smell, and the breathing to people, digestion, angiocarpy, endocrine and nervous system all have an impact；It is long
Phase, which lives in, can also cause the functional diseases such as apocleisis, insomnia, decrease of memory in odor pollution environment；Suck stench formaldehyde and
Benzene has strong carcinogenesis；High concentration stench can also make contactee occur pulmonary edema even death by suffocation.Therefore, plant dislikes
The reasonable processing of odour, is to control plant's environmental sanitation, improve epidemic prevention condition and reduce the important step of environmental pollution,
It is to ensure the important measures that livestock and poultry breeding industry develops in a healthy way.
At present, the method that processing foul gas is used mainly has combustion method, oxidizing process, absorption process, absorption method, neutralization deodorization
Method etc. is a variety of, but all there is the deficiencies such as treatment effeciency is low, produce secondary pollution, the requirement to equipment is high, not easy to operate.Therefore,
Seek the advanced subject that efficient, environmentally friendly deodorizing method is current domestic odor pollution Controlling research, one of them is exactly micro- life
Thing deodorization process.This is due to that microorganism can not only decompose feces of livestock and poultry and produce ammonia, hydrogen sulfide and methane during the fermentation
Deng foul gas, and a variety of inorganic acids can be produced, the sour environment for being unfavorable for putrefactive microorganisms life is formed, and from basic
Upper degraded produces the material of foul gas.Therefore, microbial deodorant method has traditional physical chemistry deodorizing method incomparable
Superiority, progressively highlight leading role in stench or toxic and harmful gas pollution control technology field.
Microbe deodorant processing plant stench, can be divided into regulation and control in vivo and in vitro two kinds of forms of regulation and control.At present
In terms of regulation and control in vivo are concentrated in the research of most scholars, such as using microorganism as feed addictive, to increase disappearing for forage protein
Change absorption so as to reduce foul smell discharge, but regulation and control belong to indirect deodorization in vivo, DeGrain is especially used as feed using microorganism
Additive, it is also possible to potential pathogenic, substitute and shift antibiotics resistance gene, upsets intact animal microbiota,
Produce the hidden danger such as unwanted metabolic products.The research in terms of regulation and control is concentrated mainly on single deodorization bacteria selection and deodorization in vitro
The detection of effect, and the complicated component of foul gas, including the odorant such as ammonia, hydrogen sulfide, methane and organic acid, and these
The removal of odorant needs the comprehensive function of multiple-microorganism to can be only achieved obvious effect.
The content of the invention
It is an object of the invention to provide a kind of special deodorant of livestock and poultry farm, with spray pattern handle poultry house and
The excreta of heap fermentation, in addition can also be sprayed onto livestock and poultry body surface, keep livestock and poultry body surface sanitation and hygiene, be conducive to livestock and poultry
Growth.The special deodorant of the livestock and poultry farm can not only reduce the foul gas in air, moreover it is possible to which effectively degraded produces evil
The material of odour, weakens the generation of odorant from root, with the advantage and security that conventional method is incomparable, with
Overcome conventional method exist treatment effeciency it is low, cost is high and there is the deficiencies such as certain potential safety hazard.
The technical solution adopted for the present invention to solve the technical problems is as follows：
A kind of special deodorant of livestock and poultry farm, is made up of following microorganism fungus kind：Lactobacillus acidophilus 106~108Individual/ml,
Lactobacillus reuteri 107~109Individual/ml, bacillus polymyxa 104~106Individual/ml, bacillus lentus 106~108Individual/ml, condensation
Bacillus 106~109Individual/ml, enterococcus faecalis 104~106Individual/ml, VREF 107~109Individual/ml, fold candida bacterium
104~107Individual/ml, Candida valida bacterium 104~107Individual/ml, Co-158 strain 104~106Individual/ml, the red false unit cell in marsh
Bacterium 106~108Individual/ml, Geotrichum Suaveolens 104~106Individual/ml, Aspergillusclavatus 106~108Individual/ml, rough spore aspergillus 106~108Individual/ml and ball
Spore streptomycete 106~109Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：8 ~ 10g of beef extract, 8 ~ 10g of peptone, 4 ~ 6g of yeast extract, 3 ~ 5g of glucose, Tween 80
0.5ml, 3 ~ 5g of sodium acetate, 2 ~ 4g of sodium citrate, 1 ~ 2g of dipotassium hydrogen phosphate, 0.4 ~ 0.6g of magnesium sulfate, 0.2 ~ 0.3g of manganese sulfate, sulphur
0.1 ~ 0.2g of sour ferrous iron, distilled water 1000g；All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：Two parts of 5 ~ 10ml of the culture medium being formulated by above-mentioned raw materials are measured to be fitted into two test tubes,
Under aseptic condition, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes,
6 ~ 12h of shaken cultivation under the conditions of 30 ~ 35 DEG C, adds 0.5 ~ 1g of plant extracts Jing Guo sterilization treatment afterwards, continue culture 12 ~
24h, obtains the culture of lactobacillus acidophilus and lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, are containing 1 ~ 3% chlorine
Change and 12 ~ 24h is soaked in sodium solution, soaking temperature is 35 ~ 37 DEG C, immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box
Interior drying, makes its moisture be less than 12%, drying temperature is 55 ~ 65 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；
The ethanol of addition 80 ~ 85% into powder, 3 ~ 5h of boiling, takes filtrate at a temperature of 60 ~ 70 DEG C, and filtrate is concentrated, and spraying is dry
It is dry, crush, cross 60 mesh sieves, produce plant extracts；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：25 ~ 30g of beef extract, 5 ~ 8g of peptone, 15 ~ 20g of glucose, 3 ~ 5g of sodium chloride, phosphoric acid
2 ~ 3g of disodium hydrogen, 0.2 ~ 0.4g of magnesium chloride, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare bacillus polymyxa nutrient solution, bacillus lentus culture
Liquid and bacillus coagulans nutrient solution, are distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa training on picking culture dish
In the test tube of nutrient solution, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C
24 ~ 36h, respectively obtains the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：3 ~ 5g of beef extract, 10 ~ 15g of peptone, 3 ~ 5g of sodium chloride, 8 ~ 10g of sucrose, phosphoric acid hydrogen two
1 ~ 2g of potassium, distilled water 1000g are constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, dispense
Enter test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and dung intestines ball
Bacterium is respectively connected in the test tube equipped with enterococcus faecalis nutrient solution and VREF nutrient solution, and vibration training is carried out under the conditions of 26 ~ 37 DEG C
24 ~ 36h is supported, the liquid culture of each bacterium is respectively obtained；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：2 ~ 4g of yeast extract, 4 ~ 6g of peptone, 3 ~ 6g of glucose, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, wrinkled with oese picking culture dish
Pleat candidiasis and Candida valida bacterium are respectively connected to that fold candida bacteria culture fluid and Candida valida bacterium are housed
In the test tube of nutrient solution, 24 ~ 36h of shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：2 ~ 4g of ammonium chloride, 0.5 ~ 1g of dipotassium hydrogen phosphate, 0.2 ~ 0.4g of magnesium chloride, sodium chloride 2 ~
4g, 0.5 ~ 1g of yeast extract, 1 ~ 2g of magnesium sulfate, 0.4 ~ 0.6g of sodium thiosulfate, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture
Liquid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with fermented flour on oese picking culture dish
Monad and Rhodopseudomonas palustris are respectively connected to that Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution are housed
Test tube in, under the conditions of 26 ~ 37 DEG C carry out 24 ~ 36h of shaken cultivation, respectively obtain the liquid culture of each bacterium；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：25 ~ 30g of sucrose, 10 ~ 15g of peptone, 0.5 ~ 1g of magnesium sulfate, 0.5 ~ 1g of potassium chloride, sulphur
0.01 ~ 0.03g of sour ferrous iron, 1 ~ 2g of dipotassium hydrogen phosphate, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and ball spore strepto-
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruit on oese picking culture dish
Fragrantly mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough
In the test tube of spore aspergillus nutrient solution and styreptomyces globispotus strain nutrient solution, 24 ~ 36h of shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C, respectively
To the liquid culture of each bacterium；
A kind of preparation method of the special deodorant of livestock and poultry farm is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached that above-mentioned strain is dense
Degree, is well mixed；
The livestock and poultry deodorant special culture solution is by following material composition：10 ~ 15g of beef extract, 3 ~ 5g of peptone, maltose
3 ~ 5g, 2 ~ 4g of glucose, 1 ~ 2g of sodium chloride, 1 ~ 2g of potassium dihydrogen phosphate, 2 ~ 4g of ferrous sulfate, 1 ~ 2g of magnesium sulfate, 1 ~ 2g of zinc sulfate,
Distilled water 1000g.
A kind of application method of the special deodorant of livestock and poultry farm：Sooner or later it is each in poultry house daily to spray once, daily
0.3 ~ 0.6kg/m of consumption3。
Beneficial effect
The present invention is on the foul smell and pig body suitable for empty face in processing livestock and poultry farm house ground excreta, house
Excrement is dirty, can effectively reduce the foul gas in air, and reduces the burst size of foul gas from root, is that livestock and poultry farm is disliked
Smelly processing and environmental protection provide technical support.The present invention is compared with conventional method, with following advantage：
（1）Odor removal efficient is high：Microorganism fungus kind used in the present invention is obtained by screening, by organic assembling,
Deodorizing effect is greatly improved, effectively the burst size of reduction livestock and poultry farm foul gas；
（2）Safety and environmental protection：The present invention is free of chemicals, uses pure microbial treatment method, does not result in secondary
Pollution, and each strain is conventional strain, safety is secure；
（3）The strong adaptability of strain：Organic assembling and the meticulous preparation of nutrient solution by a variety of strains, it is ensured that deodorization
Agent can remain efficient deodorizing effect under the conditions of normality；
（4）Play a role big：The excrement that the present invention both can be used for handling in poultry house is dirty, effectively can degrade again in poultry house
Foul gas in air.Simultaneously as deodorant liquid is not almost stimulated pig body into neutrality, it is also sprayable to arrive pig body surface face,
Livestock and poultry body surface sanitation and hygiene are kept, are conducive to the growth of livestock and poultry.
Specific embodiment
The present invention is further described below by way of specific embodiment.
Embodiment 1
A kind of special deodorant of livestock and poultry farm, is made up of following microorganism fungus kind：Lactobacillus acidophilus 106Individual/ml, Roy
Family name's lactobacillus 107Individual/ml, bacillus polymyxa 104Individual/ml, bacillus lentus 106Individual/ml, bacillus coagulans 106Individual/
Ml, enterococcus faecalis 104Individual/ml, VREF 107Individual/ml, fold candida bacterium 104Individual/ml, Candida valida bacterium 104
Individual/ml, Co-158 strain 104Individual/ml, Rhodopseudomonas palustris 106Individual/ml, Geotrichum Suaveolens 104Individual/ml, Aspergillusclavatus 106
Individual/ml, rough spore aspergillus 106Individual/ml and styreptomyces globispotus strain 106Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：Beef extract 8g, peptone 8g, yeast extract 4g, glucose 3g, Tween 80 0.5ml, acetic acid
Sodium 3g, sodium citrate 2g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.4g, manganese sulfate 0.2g, ferrous sulfate 0.1g, distilled water 1000g；
All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：Measure the two parts of culture medium being formulated by above-mentioned raw materials 5ml to be fitted into two test tubes, in nothing
Under the conditions of bacterium, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes, 30
Shaken cultivation 6h under the conditions of DEG C, adds the plant extracts 0.5g Jing Guo sterilization treatment afterwards, continues to cultivate 12h, obtains acidophilus
The culture of lactobacillus and lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, are containing 1% chlorination
12h is soaked in sodium solution, soaking temperature is 35 DEG C, and immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box and dried,
Its moisture is set to be less than 12%, drying temperature is 55 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；Into powder
The ethanol of addition 80%, boiling 3h, takes filtrate at a temperature of 60 DEG C, and filtrate is concentrated, and is spray-dried, and crushes, and crosses 60 mesh sieves, i.e.,
Obtain plant extracts；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：Beef extract 25g, peptone 5g, glucose 15g, sodium chloride 3g, disodium hydrogen phosphate 2g, chlorine
Change magnesium 0.2g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare bacillus polymyxa nutrient solution, bacillus lentus culture
Liquid and bacillus coagulans nutrient solution, are distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa training on picking culture dish
In the test tube of nutrient solution, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation is carried out under the conditions of 26 DEG C
24h, respectively obtains the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：Beef extract 3g, peptone 10g, sodium chloride 3g, sucrose 8, dipotassium hydrogen phosphate 1g, distilled water
1000g is constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, dispense
Enter test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and dung intestines ball
Bacterium is respectively connected in the test tube equipped with enterococcus faecalis nutrient solution and VREF nutrient solution, and shaken cultivation is carried out under the conditions of 26 DEG C
24h, respectively obtains the liquid culture of each bacterium；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：Yeast extract 2g, peptone 4g, glucose 3g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, wrinkled with oese picking culture dish
Pleat candidiasis and Candida valida bacterium are respectively connected to that fold candida bacteria culture fluid and Candida valida bacterium are housed
In the test tube of nutrient solution, shaken cultivation 24h is carried out under the conditions of 26 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：Ammonium chloride 2g, dipotassium hydrogen phosphate 0.5g, magnesium chloride 0.2g, sodium chloride 2g, yeast extract
0.5g, magnesium sulfate 1g, sodium thiosulfate 0.4g, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture
Liquid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with fermented flour on oese picking culture dish
Monad and Rhodopseudomonas palustris are respectively connected to that Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution are housed
Test tube in, under the conditions of 26 DEG C carry out shaken cultivation 24h, respectively obtain the liquid culture of each bacterium；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：Sucrose 25g, peptone 10g, magnesium sulfate 0.5g, potassium chloride 0.5g, ferrous sulfate
0.01g, dipotassium hydrogen phosphate 1g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and ball spore strepto-
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruit on oese picking culture dish
Fragrantly mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough
In the test tube of spore aspergillus nutrient solution and styreptomyces globispotus strain nutrient solution, shaken cultivation 24h is carried out under the conditions of 26 DEG C, each bacterium is respectively obtained
Liquid culture；
A kind of preparation method of the special deodorant of livestock and poultry farm is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached that above-mentioned strain is dense
Degree, is well mixed；
The livestock and poultry deodorant special culture solution is by following material composition：Beef extract 10g, peptone 3g, maltose 3g, Portugal
Grape sugar 2g, sodium chloride 1g, potassium dihydrogen phosphate 1g, ferrous sulfate 2g, magnesium sulfate 1g, zinc sulfate 1g, distilled water 1000g.
A kind of application method of the special deodorant of livestock and poultry farm：Sooner or later it is each in poultry house daily to spray once, daily
Consumption 0.3kg/m3。
Embodiment 2
A kind of special deodorant of livestock and poultry farm, is made up of following microorganism fungus kind：Lactobacillus acidophilus 108Individual/ml, Roy
Family name's lactobacillus 109Individual/ml, bacillus polymyxa 106Individual/ml, bacillus lentus 108Individual/ml, bacillus coagulans 109Individual/
Ml, enterococcus faecalis 106Individual/ml, VREF 109Individual/ml, fold candida bacterium 107Individual/ml, Candida valida bacterium 107
Individual/ml, Co-158 strain 106Individual/ml, Rhodopseudomonas palustris 108Individual/ml, Geotrichum Suaveolens 106Individual/ml, Aspergillusclavatus 108
Individual/ml, rough spore aspergillus 108Individual/ml and styreptomyces globispotus strain 109Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：Beef extract 10g, peptone 10g, yeast extract 6g, glucose 5g, Tween 80 0.5ml, vinegar
Sour sodium 5g, sodium citrate 4g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.6g, manganese sulfate 0.3g, ferrous sulfate 0.2g, distilled water
1000g；All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：The two parts of culture medium being formulated by above-mentioned raw materials 10ml are measured to be fitted into two test tubes,
Under aseptic condition, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes,
Shaken cultivation 12h under the conditions of 35 DEG C, adds the plant extracts 1g Jing Guo sterilization treatment afterwards, continues to cultivate 24h, obtains acidophilus
The culture of lactobacillus and lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, are containing 3% chlorination
24h is soaked in sodium solution, soaking temperature is 37 DEG C, and immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box and dried,
Its moisture is set to be less than 12%, drying temperature is 65 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；Into powder
The ethanol of addition 85%, boiling 5h, takes filtrate at a temperature of 70 DEG C, and filtrate is concentrated, and is spray-dried, and crushes, and crosses 60 mesh sieves, i.e.,
Obtain plant extracts；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：Beef extract 30g, peptone 8g, glucose 20g, sodium chloride 5g, disodium hydrogen phosphate 3g, chlorine
Change magnesium 0.4g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare bacillus polymyxa nutrient solution, bacillus lentus culture
Liquid and bacillus coagulans nutrient solution, are distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa training on picking culture dish
In the test tube of nutrient solution, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation is carried out under the conditions of 37 DEG C
36h, respectively obtains the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：Beef extract 5g, peptone 15g, sodium chloride 5g, sucrose 10g, dipotassium hydrogen phosphate 2g, distillation
Water 1000g is constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, dispense
Enter test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and dung intestines ball
Bacterium is respectively connected in the test tube equipped with enterococcus faecalis nutrient solution and VREF nutrient solution, and shaken cultivation is carried out under the conditions of 37 DEG C
36h, respectively obtains the liquid culture of each bacterium；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：Yeast extract 4g, peptone 6g, glucose 6g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, wrinkled with oese picking culture dish
Pleat candidiasis and Candida valida bacterium are respectively connected to that fold candida bacteria culture fluid and Candida valida bacterium are housed
In the test tube of nutrient solution, shaken cultivation 36h is carried out under the conditions of 37 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：Ammonium chloride 4g, dipotassium hydrogen phosphate 1g, magnesium chloride 0.4g, sodium chloride 4g, yeast extract 1g, sulphur
Sour magnesium 2g, sodium thiosulfate 0.6g, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture
Liquid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with fermented flour on oese picking culture dish
Monad and Rhodopseudomonas palustris are respectively connected to that Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution are housed
Test tube in, under the conditions of 37 DEG C carry out shaken cultivation 36h, respectively obtain the liquid culture of each bacterium；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：Sucrose 30g, peptone 15g, magnesium sulfate 1g, potassium chloride 1g, ferrous sulfate 0.03g, phosphorus
Sour hydrogen dipotassium 2g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and ball spore strepto-
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruit on oese picking culture dish
Fragrantly mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough
In the test tube of spore aspergillus nutrient solution and styreptomyces globispotus strain nutrient solution, shaken cultivation 36h is carried out under the conditions of 37 DEG C, each bacterium is respectively obtained
Liquid culture；
A kind of preparation method of the special deodorant of livestock and poultry farm is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached that above-mentioned strain is dense
Degree, is well mixed；
The livestock and poultry deodorant special culture solution is by following material composition：Beef extract 15g, peptone 5g, maltose 5g, Portugal
Grape sugar 4g, sodium chloride 2g, potassium dihydrogen phosphate 2g, ferrous sulfate 4g, magnesium sulfate 2g, zinc sulfate 2g, distilled water 1000g.
A kind of application method of the special deodorant of livestock and poultry farm：Sooner or later it is each in poultry house daily to spray once, daily
Consumption 0.6kg/m3。
Embodiment 3
A kind of special deodorant of livestock and poultry farm, is made up of following microorganism fungus kind：Lactobacillus acidophilus 107Individual/ml, Roy
Family name's lactobacillus 108Individual/ml, bacillus polymyxa 105Individual/ml, bacillus lentus 107Individual/ml, bacillus coagulans 107Individual/
Ml, enterococcus faecalis 105Individual/ml, VREF 108Individual/ml, fold candida bacterium 105Individual/ml, Candida valida bacterium 105
Individual/ml, Co-158 strain 105Individual/ml, Rhodopseudomonas palustris 107Individual/ml, Geotrichum Suaveolens 105Individual/ml, Aspergillusclavatus 107
Individual/ml, rough spore aspergillus 107Individual/ml and styreptomyces globispotus strain 107Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：Beef extract 9g, peptone 9g, yeast extract 5g, glucose 4g, Tween 80 0.5ml, acetic acid
Sodium 4g, sodium citrate 3g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 0.5g, manganese sulfate 0.25g, ferrous sulfate 0.15g, distilled water
1000g；All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：Measure the two parts of culture medium being formulated by above-mentioned raw materials 8ml to be fitted into two test tubes, in nothing
Under the conditions of bacterium, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes, 33
Shaken cultivation 10h under the conditions of DEG C, adds the plant extracts 0.6g Jing Guo sterilization treatment afterwards, continues to cultivate 18h, obtains acidophilus
The culture of lactobacillus and lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, are containing 2% chlorination
18h is soaked in sodium solution, soaking temperature is 36 DEG C, and immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box and dried,
Its moisture is set to be less than 12%, drying temperature is 60 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；Into powder
The ethanol of addition 82%, boiling 4h, takes filtrate at a temperature of 65 DEG C, and filtrate is concentrated, and is spray-dried, and crushes, and crosses 60 mesh sieves, i.e.,
Obtain plant extracts；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：Beef extract 26g, peptone 6g, glucose 16g, sodium chloride 4g, disodium hydrogen phosphate 2.5g,
Magnesium chloride 0.3g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare bacillus polymyxa nutrient solution, bacillus lentus culture
Liquid and bacillus coagulans nutrient solution, are distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa training on picking culture dish
In the test tube of nutrient solution, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation is carried out under the conditions of 30 DEG C
30h, respectively obtains the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：Beef extract 4g, peptone 12g, sodium chloride 4g, sucrose 9g, dipotassium hydrogen phosphate 1.5g, steaming
Distilled water 1000g is constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, dispense
Enter test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and dung intestines ball
Bacterium is respectively connected in the test tube equipped with enterococcus faecalis nutrient solution and VREF nutrient solution, and shaken cultivation is carried out under the conditions of 30 DEG C
30h, respectively obtains the liquid culture of each bacterium；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：Yeast extract 3g, peptone 5g, glucose 5g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and Candida valida
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, wrinkled with oese picking culture dish
Pleat candidiasis and Candida valida bacterium are respectively connected to that fold candida bacteria culture fluid and Candida valida bacterium are housed
In the test tube of nutrient solution, shaken cultivation 30h is carried out under the conditions of 30 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：Ammonium chloride 3g, dipotassium hydrogen phosphate 0.6g, magnesium chloride 0.3g, sodium chloride 3g, yeast extract
0.6g, magnesium sulfate 1.5g, sodium thiosulfate 0.5g, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris culture
Liquid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with fermented flour on oese picking culture dish
Monad and Rhodopseudomonas palustris are respectively connected to that Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution are housed
Test tube in, under the conditions of 30 DEG C carry out shaken cultivation 30h, respectively obtain the liquid culture of each bacterium；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：Sucrose 26g, peptone 16g, magnesium sulfate 0.6g, potassium chloride 0.6g, ferrous sulfate
0.02g, dipotassium hydrogen phosphate 1.5g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and ball spore strepto-
Bacteria culture fluid, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruit on oese picking culture dish
Fragrantly mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough
In the test tube of spore aspergillus nutrient solution and styreptomyces globispotus strain nutrient solution, shaken cultivation 30h is carried out under the conditions of 30 DEG C, each bacterium is respectively obtained
Liquid culture；
A kind of preparation method of the special deodorant of livestock and poultry farm is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached that above-mentioned strain is dense
Degree, is well mixed；
The livestock and poultry deodorant special culture solution is by following material composition：Beef extract 12g, peptone 4g, maltose 4g, Portugal
Grape sugar 3g, sodium chloride 1.5g, potassium dihydrogen phosphate 1.5g, ferrous sulfate 3g, magnesium sulfate 1.5g, zinc sulfate 1.5g, distilled water
1000g。
A kind of application method of the special deodorant of livestock and poultry farm：Sooner or later it is each in poultry house daily to spray once, daily
Consumption 0.4kg/m3。
Test effect：The deodorant living bacteria count of the present invention is more, good deodorization effect.Prepared with 1-3 of the embodiment of the present invention
Deodorant in twins（Group）The plant of limited company subordinate is tested, and as a result shows NH in excrement3With
H2S burst sizes decline NH in 55 ~ 75% and 30 ~ 40%, the deodorant best results that wherein embodiment 3 is prepared, excrement respectively3And H2S
Burst size declines 75% and 40% respectively.
Claims (6)
1. a kind of special deodorant of livestock and poultry farm, it is characterised in that be made up of following microorganism fungus kind：Lactobacillus acidophilus 106~
108Individual/ml, lactobacillus reuteri 107~109Individual/ml, bacillus polymyxa 104~106Individual/ml, bacillus lentus 106~108
Individual/ml, bacillus coagulans 106~109Individual/ml, enterococcus faecalis 104~106Individual/ml, VREF 107~109Individual/ml, gauffer are false
Silk saccharomycete 104~107Individual/ml, Candida valida bacterium 104~107Individual/ml, Co-158 strain 104~106Individual/ml, marsh
Red pseudomonas 106~108Individual/ml, Geotrichum Suaveolens 104~106Individual/ml, Aspergillusclavatus 106~108Individual/ml, rough spore aspergillus 106~108
Individual/ml and styreptomyces globispotus strain 106~109Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：8 ~ 10g of beef extract, 8 ~ 10g of peptone, 4 ~ 6g of yeast extract, 3 ~ 5g of glucose, tween
800.5ml, 3 ~ 5g of sodium acetate, 2 ~ 4g of sodium citrate, 1 ~ 2g of dipotassium hydrogen phosphate, 0.4 ~ 0.6g of magnesium sulfate, 0.2 ~ 0.3g of manganese sulfate,
0.1 ~ 0.2g of ferrous sulfate, distilled water 1000g；All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：Measure two parts of 5 ~ 10ml of the culture medium being formulated by above-mentioned raw materials to be fitted into two test tubes, sterile
Under the conditions of, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes, 30 ~ 35
6 ~ 12h of shaken cultivation under the conditions of DEG C, adds 0.5 ~ 1g of plant extracts Jing Guo sterilization treatment afterwards, continues to cultivate 12 ~ 24h,
Obtain the culture of lactobacillus acidophilus and lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, are containing 1 ~ 3% sodium chloride
12 ~ 24h is soaked in solution, soaking temperature is 35 ~ 37 DEG C, and immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box and dried
It is dry, its moisture is less than 12%, drying temperature is 55 ~ 65 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；
The ethanol of addition 80 ~ 85% into powder, 3 ~ 5h of boiling, takes filtrate at a temperature of 60 ~ 70 DEG C, and filtrate is concentrated, spraying
It dry, pulverize, cross 60 mesh sieves, produce plant extracts；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：25 ~ 30g of beef extract, 5 ~ 8g of peptone, 15 ~ 20g of glucose, 3 ~ 5g of sodium chloride, phosphoric acid hydrogen two
2 ~ 3g of sodium, 0.2 ~ 0.4g of magnesium chloride, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material prepare bacillus polymyxa nutrient solution, bacillus lentus nutrient solution and
Bacillus coagulans nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese picking is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa culture on culture dish
In the test tube of liquid, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation 24 is carried out under the conditions of 26 ~ 37 DEG C
~ 36h, respectively obtains the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：3 ~ 5g of beef extract, 10 ~ 15g of peptone, 3 ~ 5g of sodium chloride, 8 ~ 10g of sucrose, dipotassium hydrogen phosphate 1 ~
2g, distilled water 1000g are constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, be distributed into examination
Manage, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and VREF point
Jie Ru equipped with carried out in the test tube of enterococcus faecalis nutrient solution and VREF nutrient solution, under the conditions of 26 ~ 37 DEG C shaken cultivation 24 ~
36h, respectively obtains the liquid culture of each bacterium；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：2 ~ 4g of yeast extract, 4 ~ 6g of peptone, 3 ~ 6g of glucose, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and the training of Candida valida bacterium
Nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with gauffer on oese picking culture dish
Silk saccharomycete and Candida valida bacterium are respectively connected to cultivate equipped with fold candida bacteria culture fluid and Candida valida bacterium
In the test tube of liquid, 24 ~ 36h of shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：2 ~ 4g of ammonium chloride, 0.5 ~ 1g of dipotassium hydrogen phosphate, 0.2 ~ 0.4g of magnesium chloride, 2 ~ 4g of sodium chloride, ferment
Female 0.5 ~ 1g of cream, 1 ~ 2g of magnesium sulfate, 0.4 ~ 0.6g of sodium thiosulfate, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris nutrient solution, point
Load test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with Co-158 strain on oese picking culture dish
The test tube equipped with Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution is respectively connected to Rhodopseudomonas palustris
In, 24 ~ 36h of shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C, the liquid culture of each bacterium is respectively obtained；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：25 ~ 30g of sucrose, 10 ~ 15g of peptone, 0.5 ~ 1g of magnesium sulfate, 0.5 ~ 1g of potassium chloride, sulfuric acid are sub-
0.01 ~ 0.03g of iron, 1 ~ 2g of dipotassium hydrogen phosphate, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain training
Nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruity on oese picking culture dish
Mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to bent equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough spore
In the test tube of mould nutrient solution and styreptomyces globispotus strain nutrient solution, 24 ~ 36h of shaken cultivation is carried out under the conditions of 26 ~ 37 DEG C, is respectively obtained each
The liquid culture of bacterium.
2. the special deodorant of a kind of livestock and poultry farm according to claim 1, it is characterised in that by following microorganism fungus kind group
Into：Lactobacillus acidophilus 107Individual/ml, lactobacillus reuteri 108Individual/ml, bacillus polymyxa 105Individual/ml, bacillus lentus
107Individual/ml, bacillus coagulans 107Individual/ml, enterococcus faecalis 105Individual/ml, VREF 108Individual/ml, fold candida bacterium
105Individual/ml, Candida valida bacterium 105Individual/ml, Co-158 strain 105Individual/ml, Rhodopseudomonas palustris 107Individual/ml,
Geotrichum Suaveolens 105Individual/ml, Aspergillusclavatus 107Individual/ml, rough spore aspergillus 107Individual/ml and styreptomyces globispotus strain 107Individual/ml；
The lactobacillus acidophilus and the preparation method of lactobacillus reuteri：
（1）The composition of culture medium：Beef extract 9g, peptone 9g, yeast extract 5g, glucose 4g, Tween 80 0.5ml, sodium acetate
4g, sodium citrate 3g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 0.5g, manganese sulfate 0.25g, ferrous sulfate 0.15g, distilled water
1000g；All culture medium raw materials are carrying out sterilization treatment using preceding；
（2）Fermented and cultured：Measure the two parts of culture medium being formulated by above-mentioned raw materials 8ml to be fitted into two test tubes, in sterile bar
Under part, the lactobacillus acidophilus on picking culture dish is distinguished with oese and lactobacillus reuteri is accessed in two test tubes, 33 DEG C of bars
Shaken cultivation 10h under part, adds the plant extracts 0.6g Jing Guo sterilization treatment afterwards, continues to cultivate 18h, obtains the newborn bar of acidophilus
Bacterium and the culture of lactobacillus reuteri；
The preparation method of the plant extracts is：By matrimony vine and mulberries in mass ratio 1:2 mixing, molten containing 2% sodium chloride
18h is soaked in liquid, soaking temperature is 36 DEG C, and immersion is filtered after terminating, and takes filter residue；Filter residue is placed in drying box and dried, makes it
Moisture is less than 12%, and drying temperature is 60 DEG C, is crushed, and crosses 60 mesh sieves, obtains the powder of matrimony vine and mulberries；Added into powder
82% ethanol, boiling 4h, takes filtrate at a temperature of 65 DEG C, and filtrate is concentrated, and is spray-dried, and crushes, and crosses 60 mesh sieves, produces plant
Thing extract；
The preparation method of the bacillus polymyxa, bacillus lentus and bacillus coagulans：
（1）The composition of culture medium：Beef extract 26g, peptone 6g, glucose 16g, sodium chloride 4g, disodium hydrogen phosphate 2.5g, chlorination
Magnesium 0.3g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material prepare bacillus polymyxa nutrient solution, bacillus lentus nutrient solution and
Bacillus coagulans nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, oese picking is used
Bacillus polymyxa, bacillus lentus and bacillus coagulans are respectively connected to equipped with bacillus polymyxa culture on culture dish
In the test tube of liquid, bacillus lentus nutrient solution and bacillus coagulans nutrient solution, shaken cultivation 30h is carried out under the conditions of 30 DEG C,
Respectively obtain the liquid culture of each bacterium；
The enterococcus faecalis and the preparation method of VREF：
（1）The composition of culture medium：Beef extract 4g, peptone 12g, sodium chloride 4g, sucrose 9g, dipotassium hydrogen phosphate 1.5g, distilled water
1000g is constituted；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare enterococcus faecalis nutrient solution and VREF nutrient solution, be distributed into examination
Manage, often pipe 5ml, sterilizing is saved backup；Aseptically, with enterococcus faecalis on oese picking culture dish and VREF point
In the test tube that enterococcus faecalis nutrient solution and VREF nutrient solution Jie Ru be housed, shaken cultivation 30h is carried out under the conditions of 30 DEG C, point
The liquid culture of each bacterium is not obtained；
The preparation method of the fold candida bacterium and Candida valida bacterium：
（1）The composition of culture medium：Yeast extract 3g, peptone 5g, glucose 5g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare fold candida bacteria culture fluid and the training of Candida valida bacterium
Nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, it is false with gauffer on oese picking culture dish
Silk saccharomycete and Candida valida bacterium are respectively connected to cultivate equipped with fold candida bacteria culture fluid and Candida valida bacterium
In the test tube of liquid, shaken cultivation 30h is carried out under the conditions of 30 DEG C, the liquid culture of each bacterium is respectively obtained；
The preparation method of the Co-158 strain and Rhodopseudomonas palustris：
（1）The composition of culture medium：Ammonium chloride 3g, dipotassium hydrogen phosphate 0.6g, magnesium chloride 0.3g, sodium chloride 3g, yeast extract 0.6g, sulphur
Sour magnesium 1.5g, sodium thiosulfate 0.5g, distilled water 1000g compositions；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Co-158 strain and Rhodopseudomonas palustris nutrient solution, point
Load test tube, often pipe 5ml, sterilizing is saved backup；Aseptically, with Co-158 strain on oese picking culture dish
The test tube equipped with Co-158 strain nutrient solution and Rhodopseudomonas palustris nutrient solution is respectively connected to Rhodopseudomonas palustris
In, shaken cultivation 30h is carried out under the conditions of 30 DEG C, the liquid culture of each bacterium is respectively obtained；
The Geotrichum Suaveolens, Aspergillusclavatus, the preparation method of rough spore aspergillus and styreptomyces globispotus strain：
（1）The composition of culture medium：Sucrose 26g, peptone 16g, magnesium sulfate 0.6g, potassium chloride 0.6g, ferrous sulfate 0.02g, phosphorus
Sour hydrogen dipotassium 1.5g, distilled water 1000g；
（2）Fermented and cultured：Weigh above-mentioned culture medium raw material and prepare Geotrichum Suaveolens, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain training
Nutrient solution, is distributed into test tube, often pipe 5ml, and sterilizing is saved backup；Aseptically, with fruity on oese picking culture dish
Mould, Aspergillusclavatus, rough spore aspergillus and styreptomyces globispotus strain are respectively connected to bent equipped with Geotrichum Suaveolens nutrient solution, Aspergillusclavatus nutrient solution, rough spore
In the test tube of mould nutrient solution and styreptomyces globispotus strain nutrient solution, shaken cultivation 30h is carried out under the conditions of 30 DEG C, the liquid of each bacterium is respectively obtained
Body culture.
3. according to a kind of any special deodorant of livestock and poultry farm of claim 1-2, it is characterised in that preparation method is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached above-mentioned strain concentration, is mixed
Close uniform；
The livestock and poultry deodorant special culture solution is by following material composition：10 ~ 15g of beef extract, 3 ~ 5g of peptone, maltose 3 ~
5g, 2 ~ 4g of glucose, 1 ~ 2g of sodium chloride, 1 ~ 2g of potassium dihydrogen phosphate, 2 ~ 4g of ferrous sulfate, 1 ~ 2g of magnesium sulfate, 1 ~ 2g of zinc sulfate, steaming
Distilled water 1000g.
4. the special deodorant of a kind of livestock and poultry farm according to claim 3, it is characterised in that preparation method is as follows：
The liquid culture of each bacterium is added in livestock and poultry deodorant special culture solution, each bacterium is reached above-mentioned strain concentration, is mixed
Close uniform；
The livestock and poultry deodorant special culture solution is by following material composition：Beef extract 12g, peptone 4g, maltose 4g, glucose
3g, sodium chloride 1.5g, potassium dihydrogen phosphate 1.5g, ferrous sulfate 3g, magnesium sulfate 1.5g, zinc sulfate 1.5g, distilled water 1000g.
5. according to a kind of any special deodorant of livestock and poultry farm of claim 1-2, it is characterised in that application method：Daily
Sooner or later it is each in poultry house to spray once, daily 0.3 ~ 0.6kg/m of consumption3。
6. a kind of special deodorant of livestock and poultry farm according to claim 5, it is characterised in that application method：Exist sooner or later daily
It is each in poultry house to spray once, daily consumption 0.4kg/m3。
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| CN111821847A (en) * | 2020-07-28 | 2020-10-27 | 湖南艾凯环保科技有限公司 | Composite biological deodorant and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102688516B (en) * | 2011-11-30 | 2013-12-25 | 河南科技大学 | Deodorizing and dedusting agent for chicken farm and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247611A (en) * | 2011-07-07 | 2011-11-23 | 贵州大学 | Microorganism deodorant and preparation method thereof |
| CN102688516B (en) * | 2011-11-30 | 2013-12-25 | 河南科技大学 | Deodorizing and dedusting agent for chicken farm and preparation method thereof |
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