CN108499353B - Special deodorant for large-scale pig farm and application of special deodorant - Google Patents
Special deodorant for large-scale pig farm and application of special deodorant Download PDFInfo
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Abstract
The invention discloses a special deodorant for large-scale pig farms and application thereof, which consists of a lactobacillus raman culture, an oval bifidobacterium culture, a bifidobacterium longum culture, a streptococcus intermedius culture, a propionibacterium freudenreichii culture, an aspergillus aconiti dissociatus culture and a filamentous fungus agent, wherein the various bacteria cultures and the fungus agent are added into 6-10% of brown sugar aqueous solution to ensure that the concentration of lactobacillus raman strain reaches 105‑107The strain concentration of Bifidobacterium ovoid/ml reaches 106‑108The strain concentration of Bifidobacterium longum in each ml is 104‑106The concentration of the streptococcus intermedius strain reaches 10 per ml7‑109The concentration of the propionic acid bacteria strain reaches 10 per ml4‑108The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 104‑107The concentration of filamentous fungus strains reaches 10 per ml6‑109One per ml. The deodorant can be used for treating the live pig breeding pen house in a spraying mode, can radically and rapidly degrade malodorous gas substances such as ammonia gas, hydrogen sulfide, methane, indole, volatile fatty acid, mercaptan and the like, and cannot generate adverse effects on the health of the live pigs.
Description
Technical Field
The invention relates to a deodorant, in particular to a special deodorant for a large-scale pig farm and application thereof, and belongs to the technical field of environmental protection.
Background
With the super-scale development of the pig breeding industry, the pollution of pig excrement to the environment around the breeding farm is serious day by day, and a considerable part of the pig farm emits very unpleasant smell to seriously pollute the living environment of surrounding residents. Currently, 160 volatile components have been identified from feces. More than 80 nitrogen-containing compounds are also found in feces and urine, 10 of which are associated with malodor. In addition, ammonia, SO2, NO2, amines, amino acid derivatives, and the like are also produced during fermentation of the manure. Although the correlation between ammonia gas and feces odor is not great, a great deal of research shows that the production performance and the health condition of animals are affected by overhigh environmental ammonia gas concentration, the feed intake and the daily gain of the animals are reduced, the incidence rate of pneumonia is increased, and sexual maturity is delayed.
At present, the main methods adopted for treating the malodorous gas in the pig farm are as follows: absorption, adsorption, oxidation, combustion and the like, but have problems of low treatment efficiency, inconvenient operation, easy generation of secondary pollution and the like. The method for deodorizing by microorganisms is a method at the leading edge in recent years, and mainly comprises two forms of animal in-vivo regulation and control treatment and in-vitro regulation and control treatment, wherein the in-vivo regulation and control means that the microorganisms are added into daily ration of pigs in the form of feed additives to promote the digestion and absorption of nutrient substances by pig organisms and reduce the generation of odor, but the deodorization effect is not obvious, and the microorganisms possibly have antibiotic resistance genes to disturb the normal animal microorganism flora. The in vitro regulation and control mode mainly focuses on the screening of single deodorant strains and the detection of the deodorant effect, and the malodorous gas has complex components and unobvious effect. Therefore, how to develop a foul gas which can rapidly treat complex components in a large-scale pig farm is of great significance for improving the breeding efficiency.
Disclosure of Invention
The invention aims to provide a deodorant capable of efficiently and quickly removing malodorous gas in a large-scale pig farm, which can be treated by spraying on a live pig breeding house, can quickly degrade malodorous gas substances radically, and does not have adverse effects on the health of pigs.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows:
a special deodorant for large-scale pig farm comprises Lactobacillus raman culture, Bifidobacterium ovoid culture, Bifidobacterium longum culture, Streptococcus intermedius culture, propionic acid bacterium freudenreichii culture, Aspergillus aconiti Demonii culture, and filamentous fungus agent;
the preparation method comprises the following steps: adding the above culture and microbial inoculum into 6-10% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 105-107The strain concentration of Bifidobacterium ovoid/ml reaches 106-108The strain concentration of Bifidobacterium longum in each ml is 104-106The concentration of the streptococcus intermedius strain reaches 10 per ml7-109The concentration of the propionic acid bacteria strain reaches 10 per ml4-108The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 104-107The concentration of filamentous fungus strains reaches 10 per ml6-109One per ml.
The preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water.
The preparation method of the drynaria extract comprises the following steps: pulverizing dried rhizoma Drynariae, sieving with 60 mesh sieve, adding 30-50 times of water and 0.5-0.8% of complex enzyme, maintaining at 40-50 deg.C, leaching for 12-18 hr, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the complex enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:2-3: 1-3;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture at the temperature of 25-35 ℃ for 12-24h to obtain a Lactobacillus raman culture.
The preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) inoculating Bifidobacterium ovoid and Bifidobacterium longum into test tubes containing sterilized culture solution under sterile condition, and culturing at 25-35 deg.C for 18-32 hr under shaking to obtain Bifidobacterium ovoid culture and Bifidobacterium longum culture.
The preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing sterilized culture solution under aseptic condition with an inoculating loop, and culturing at 25-35 deg.C for 18-32h with shaking to obtain Streptococcus intermedius culture.
The preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) under the aseptic condition, inoculating the propionic acid bacterium freudenreichii into a test tube filled with the sterilized culture solution, performing shake culture at 25-35 ℃ for 4-8h, adding sterilized polygonum capitatum powder into the test tube, and continuing to perform shake culture at 20-30 ℃ for 12-18h to obtain the propionic acid bacterium freudenreichii culture.
The preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween-801 ml and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution under aseptic condition, performing shake culture at 25-35 deg.C for 10-16h, adding sterilized fructus Myricae Rubrae pulp powder into the test tube, and maintaining at 25-35 deg.C for further shake culture for 10-16h to obtain Aspergillus aconiti acidolyticus culture.
The preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 0.5-1 wt% into fresh bean dregs, mixing, steaming with strong fire for 30-40min, cooling, and squeezing to 1cm3And putting the bean dreg blocks on a reticulate pattern air-permeable plate paved with a layer of straws, keeping the temperature at 13-16 ℃ and the relative humidity at 70-75%, fermenting for 72-96h, after the fermentation is finished, picking white fuzz on the bean dreg blocks, and carrying out freeze vacuum drying to obtain the filamentous fungus agent.
The use method of the invention comprises the following steps: spraying the mixture once every morning and evening in a large-scale pigsty with daily dosage of 0.1-0.2kg/m3。
Advantageous effects
1. The deodorant has high deodorization speed and remarkable effect, after various bacteria are cultured, the beneficial bacteria can produce rich and high-activity enzymes and beneficial products, meanwhile, microbial strains effectively absorb active ingredients of Chinese herbal medicines and the like in a culture solution and are sprayed to a large-scale piggery, the ammonia removal rate reaches 80-95%, the hydrogen sulfide removal rate reaches 70-85%, the methane removal rate reaches 80-95%, the indole removal rate reaches more than 80%, the volatile fatty acid removal rate reaches more than 85%, and the mercaptan removal rate reaches more than 75% within 12 hours.
2. The allium macrostemon and the polygonum capitatum are added in the culture solution and the fermentation process of the freudenreichii, so that the production of more vitamin B12 by the freudenreichii in the culture process can be promoted, and the decomposition of odor is accelerated.
3. In the preparation process of the aspergillus aconiti anzevii, the nutrition and active ingredients of the dark plum pulp and the red bayberry pulp are absorbed, the capacity of producing the itaconic acid is greatly enhanced, the produced itaconic acid can effectively inhibit the growth of putrefying microorganisms, and the generation of malodorous gas can be fundamentally reduced.
4. In the preparation process of the filamentous fungus agent, the added folium artemisiae argyi powder can effectively purify and culture filamentous fungi, the obtained filamentous fungi have higher purity, and meanwhile, the effective components in the folium artemisiae argyi powder are absorbed, so that the deodorization effect of the agent is greatly improved.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Example 1
A special deodorant for large-scale pig farm comprises Lactobacillus raman culture, Bifidobacterium ovoid culture, Bifidobacterium longum culture, Streptococcus intermedius culture, propionic acid bacterium freudenreichii culture, Aspergillus aconiti Demonii culture, and filamentous fungus agent;
the preparation method comprises the following steps: adding the above bacteria culture and microbial inoculum into 6% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 105The strain concentration of Bifidobacterium ovoid/ml reaches 106The strain concentration of Bifidobacterium longum in each ml is 104The concentration of the streptococcus intermedius strain reaches 10 per ml7The concentration of the propionic acid bacteria strain reaches 10 per ml4The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 104The concentration of filamentous fungus strains reaches 10 per ml6One per ml.
The preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water.
The preparation method of the drynaria extract comprises the following steps: taking dried rhizoma Drynariae, pulverizing, sieving with 60 mesh sieve, adding 30 times of water and 0.5% complex enzyme, maintaining the temperature at 40 deg.C, leaching for 12 hr, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the complex enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:2: 1;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture at the temperature of 25 ℃ for 24h to obtain the Lactobacillus raman culture.
The preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) inoculating Bifidobacterium ovoid and Bifidobacterium longum into test tubes containing sterilized culture solution under sterile condition, and culturing at 25 deg.C for 32 hr under shaking to obtain Bifidobacterium ovoid culture and Bifidobacterium longum culture.
The preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing the sterilized culture solution by using an inoculating loop under aseptic conditions, and performing shake culture at 25 ℃ for 32h to obtain a Streptococcus intermedius culture.
The preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) inoculating the frey propionic acid bacteria into a test tube containing the sterilized culture solution under the aseptic condition, performing shake culture at 25 ℃ for 8h, adding sterilized polygonum capitatum powder into the test tube, and continuing to perform shake culture at 20 ℃ for 18h to obtain the frey propionic acid bacteria culture.
The preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution under aseptic condition by using an inoculating loop, performing shake culture at 25 ℃ for 16h, adding sterilized waxberry fruit powder into the test tube, and continuing shake culture at 25 ℃ for 16h to obtain Aspergillus aconiti acidolyticus culture.
The preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 0.5 wt% into fresh bean dregs, mixing, steaming with strong fire for 30min, cooling, and squeezing to 1cm3And putting the bean dreg blocks on a reticulate pattern air-permeable plate paved with a layer of straw, keeping the temperature at 13 ℃ and the relative humidity at 70%, fermenting for 96 hours, picking white fuzz on the bean dreg blocks after the fermentation is finished, and freezing and drying in vacuum to obtain the filamentous fungus agent.
The use method of the invention comprises the following steps: spraying the solution once every morning and evening in a large-scale pigsty with a daily dosage of 0.2kg/m3。
Example 2
A special deodorant for large-scale pig farm comprises Lactobacillus raman culture, Bifidobacterium ovoid culture, Bifidobacterium longum culture, Streptococcus intermedius culture, propionic acid bacterium freudenreichii culture, Aspergillus aconiti Demonii culture, and filamentous fungus agent;
the preparation method comprises the following steps: adding the above bacteria culture and microbial inoculum into 8% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 106The strain concentration of Bifidobacterium ovoid/ml reaches 107The strain concentration of Bifidobacterium longum in each ml is 105The concentration of the intermediate streptococcus strain reaches 10/ml and the concentration of the propionic acid bacterium strain reaches 106The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 105The concentration of filamentous fungus strains reaches 10 per ml8Per ml;
the preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water.
The preparation method of the drynaria extract comprises the following steps: taking dried rhizoma Drynariae, pulverizing, sieving with 60 mesh sieve, adding 40 times of water and 0.65% complex enzyme, maintaining the temperature at 45 deg.C, leaching for 15 hr, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the compound enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:2.5: 2;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture at the temperature of 30 ℃ for 18h to obtain the Lactobacillus raman culture.
The preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) inoculating Bifidobacterium ovoid and Bifidobacterium longum into test tubes containing sterilized culture solution under sterile condition, and culturing at 30 deg.C for 25 hr under shaking to obtain Bifidobacterium ovoid culture and Bifidobacterium longum culture.
The preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing the sterilized culture solution by using an inoculating loop under aseptic conditions, and performing shake culture at 30 ℃ for 25h to obtain a Streptococcus intermedius culture.
The preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) inoculating the frey propionic acid bacteria into a test tube containing the sterilized culture solution under the aseptic condition, performing shake culture at 30 ℃ for 6h, adding sterilized polygonum capitatum powder into the test tube, and continuing to perform shake culture at 25 ℃ for 15h to obtain the frey propionic acid bacteria culture.
The preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution under aseptic condition by using an inoculating loop, performing shake culture at 30 ℃ for 13h, adding sterilized waxberry fruit powder into the test tube, and continuing shake culture at 30 ℃ for 13h to obtain Aspergillus aconiti acidolyticus culture.
The preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 0.75 wt% into fresh bean dregs, and mixingMixing, steaming with strong fire for 305min, cooling, and squeezing to 1cm3And putting the bean dreg blocks on a reticulate pattern air-permeable plate paved with a layer of straw, keeping the temperature at 15 ℃ and the relative humidity at 73%, fermenting for 84h, picking white fuzz on the bean dreg blocks after fermentation is finished, and freezing and drying in vacuum to obtain the filamentous fungus agent.
The use method of the invention comprises the following steps: spraying the solution once every morning and evening in a large-scale pigsty with a daily dose of 0.15kg/m3。
Example 3
A special deodorant for large-scale pig farm comprises Lactobacillus raman culture, Bifidobacterium ovoid culture, Bifidobacterium longum culture, Streptococcus intermedius culture, propionic acid bacterium freudenreichii culture, Aspergillus aconiti Demonii culture, and filamentous fungus agent;
the preparation method comprises the following steps: adding the above bacteria culture and microbial inoculum into 10% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 107The strain concentration of Bifidobacterium ovoid/ml reaches 108The strain concentration of Bifidobacterium longum in each ml is 106The concentration of the streptococcus intermedius strain reaches 10 per ml9The concentration of the propionic acid bacteria strain reaches 10 per ml8The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 107The concentration of filamentous fungus strains reaches 10 per ml9Per ml;
the preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water.
The preparation method of the drynaria extract comprises the following steps: taking dried rhizoma Drynariae, pulverizing, sieving with 60 mesh sieve, adding 50 times of water and 0.8% of complex enzyme, maintaining the temperature at 50 deg.C, leaching for 18h, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the compound enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:3: 3;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture at the temperature of 35 ℃ for 12h to obtain the Lactobacillus raman culture.
The preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) inoculating Bifidobacterium ovoid and Bifidobacterium longum into test tubes containing sterilized culture solution under sterile condition, and culturing at 35 deg.C for 18 hr under shaking to obtain Bifidobacterium ovoid culture and Bifidobacterium longum culture.
The preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing the sterilized culture solution by using an inoculating loop under aseptic conditions, and performing shake culture at 35 ℃ for 18h to obtain a Streptococcus intermedius culture.
The preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) inoculating the frey propionic acid bacteria into a test tube containing the sterilized culture solution under the aseptic condition, performing shake culture at 35 ℃ for 4h, adding sterilized polygonum capitatum powder into the test tube, and continuing to perform shake culture at 30 ℃ for 12h to obtain the frey propionic acid bacteria culture.
The preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution under aseptic condition by using an inoculating loop, performing shake culture at 35 deg.C for 10h, adding sterilized fructus Myricae Rubrae powder into the test tube, and maintaining at 35 deg.C for further shake culture for 10h to obtain Aspergillus aconiti acidolyticus culture.
The preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 1 wt% into fresh bean dregs, mixing, steaming with strong fire for 40min, cooling, and squeezing to 1cm3And putting the bean dreg blocks on a reticulate pattern air-permeable plate paved with a layer of straw, keeping the temperature at 16 ℃ and the relative humidity at 75%, fermenting for 72 hours, picking white fuzz on the bean dreg blocks after the fermentation is finished, and freezing and drying in vacuum to obtain the filamentous fungus agent.
The use method of the invention comprises the following steps: spraying the solution once every morning and evening in a large-scale pigsty with a daily dosage of 0.1kg/m3。
Comparative example 1: the use method of the deodorant prepared according to the special deodorant for the livestock and poultry farm disclosed by the invention is as follows: spraying the solution once every morning and evening in a large-scale pigsty with a daily dosage of 0.3kg/m3 。
Comparative example 2: the use method of the deodorant prepared according to the contents disclosed by the invention of the microbial deodorant and the preparation method thereof disclosed by the Chinese patent publication No. CN102247611A is as follows: spraying the solution once every morning and evening in a large-scale pigsty with a daily dosage of 0.3kg/m3 。
The test effect is as follows:
the deodorizers of examples 1 to 3 and comparative examples 1 and 2 were sprayed to five identical houses in a pig farm belonging to the genus of Xinxing agriculture and animal husbandry, Inc., Wan Xin, Jiangxi, respectively, and the degradation of odorant was measured for 12 hours, and the results showed that: the ammonia removal rate in the spraying example 1-3 columns reaches 80-95%, the hydrogen sulfide removal rate reaches 70-85%, the methane removal rate reaches 80-95%, the indole removal rate reaches more than 80%, the volatile fatty acid removal rate reaches more than 85%, and the mercaptan removal rate reaches more than 75%, the ammonia removal rate in the spraying comparative example 1 and 2 columns respectively reaches 73% and 75%, the hydrogen sulfide removal rate respectively reaches 45% and 51%, the methane removal rate respectively reaches 68% and 65%, the indole removal rate respectively reaches 58% and 65%, the volatile fatty acid removal rate respectively reaches 72% and 66%, and the mercaptan removal rate respectively reaches 55% and 60%. The deodorant disclosed by the invention can be used for quickly and effectively removing odor gases such as ammonia gas, hydrogen sulfide, methane, indole, volatile fatty acid, mercaptan and the like in a pigsty, and has an obvious effect.
Claims (4)
1. A special deodorant for a large-scale pig farm is characterized in that: consists of a Lactobacillus raman culture, an oval bifidobacterium culture, a bifidobacterium longum culture, a streptococcus intermedius culture, a propionic acid bacterium culture, an aconitum acidolyticum culture and a filamentous fungus agent;
the preparation method comprises the following steps: adding the above culture and microbial inoculum into 6-10% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 105-107The strain concentration of Bifidobacterium ovoid/ml reaches 106-108The strain concentration of Bifidobacterium longum in each ml is 104-106The concentration of the streptococcus intermedius strain reaches 10 per ml7-109The concentration of the propionic acid bacteria strain reaches 10 per ml4-108The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 104-107The concentration of filamentous fungus strains reaches 10 per ml6-109Per ml;
the preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water;
the preparation method of the drynaria extract comprises the following steps: pulverizing dried rhizoma Drynariae, sieving with 60 mesh sieve, adding 30-50 times of water and 0.5-0.8% of complex enzyme, maintaining at 40-50 deg.C, leaching for 12-18 hr, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the complex enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:2-3: 1-3;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture at the temperature of 25-35 ℃ for 12-24h to obtain a Lactobacillus raman culture;
the preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) inoculating Bifidobacterium ovoid and Bifidobacterium longum into test tubes containing sterilized culture solution under aseptic condition, and culturing at 25-35 deg.C for 18-32 hr under shaking to obtain Bifidobacterium ovoid culture and Bifidobacterium longum culture;
the preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing sterilized culture solution under aseptic condition with an inoculating loop, and performing shake culture at 25-35 deg.C for 18-32h to obtain Streptococcus intermedius culture;
the preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) inoculating the propionic acid bacterium freudenreichii into a test tube containing the sterilized culture solution under aseptic condition, performing shake culture at 25-35 deg.C for 4-8h, adding sterilized herba Polygoni Capitati powder into the test tube, and maintaining the temperature at 20-30 deg.C for further shake culture for 12-18h to obtain propionic acid bacterium freudenreichii culture;
the preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution under aseptic condition by using an inoculating loop, performing shake culture at 25-35 deg.C for 10-16h, adding sterilized fructus Myricae Rubrae pulp powder into the test tube, and maintaining the temperature at 25-35 deg.C for further shake culture for 10-16h to obtain Aspergillus aconiti acidolyticus culture;
the preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 0.5-1 wt% into fresh bean dregs, mixing, steaming with strong fire for 30-40min, cooling, and squeezing to 1cm3And putting the bean dreg blocks on a reticulate pattern air-permeable plate paved with a layer of straws, keeping the temperature at 13-16 ℃ and the relative humidity at 70-75%, fermenting for 72-96h, after the fermentation is finished, picking white fuzz on the bean dreg blocks, and carrying out freeze vacuum drying to obtain the filamentous fungus agent.
2. The special deodorant for the large-scale pig farm according to claim 1, wherein: consists of a Lactobacillus raman culture, an oval bifidobacterium culture, a bifidobacterium longum culture, a streptococcus intermedius culture, a propionic acid bacterium culture, an aconitum acidolyticum culture and a filamentous fungus agent;
preparation method thereofThe method comprises the following steps: adding the above bacteria culture and microbial inoculum into 8% brown sugar water solution, and mixing to make the concentration of Lactobacillus raman strain reach 106The strain concentration of Bifidobacterium ovoid/ml reaches 107The strain concentration of Bifidobacterium longum in each ml is 105The concentration of the intermediate streptococcus strain reaches 10/ml and the concentration of the propionic acid bacterium strain reaches 106The concentration of the aspergillus aconiti acidolyticus strain per ml reaches 105The concentration of filamentous fungus strains reaches 10 per ml8Per ml;
the preparation method of the Lactobacillus raman culture comprises the following steps:
(1) the formula of the culture solution comprises: 4.5g of yeast extract, 9.5g of tryptone, 9.0g of beef extract, 18g of glucose, 1.8g of diammonium citrate, 800.8 ml of tween, 1.8g of monopotassium phosphate, 4.5g of sodium acetate, 0.55g of magnesium sulfate, 0.2g of manganese sulfate, 0.2g of drynaria extract and 1000ml of distilled water;
the preparation method of the drynaria extract comprises the following steps: taking dried rhizoma Drynariae, pulverizing, sieving with 60 mesh sieve, adding 40 times of water and 0.65% complex enzyme, maintaining the temperature at 45 deg.C, leaching for 15 hr, filtering, removing residue, vacuum drying the filtrate, pulverizing, and sieving with 80 mesh sieve to obtain rhizoma Drynariae extract;
the compound enzyme consists of cellulase, pectinase and protease according to the weight ratio of 1:2.5: 2;
(2) inoculating Lactobacillus raman into a test tube filled with the sterilized culture solution by using an inoculating loop under the aseptic condition, and performing shake culture for 18h at the temperature of 30 ℃ to obtain a Lactobacillus raman culture;
the preparation method of the oval bifidobacterium culture and the bifidobacterium longum culture comprises the following steps:
(1) the formula of the culture solution comprises: 20g of peptone, 1.5g of yeast powder, 20g of glucose, 0.5g of potato starch, 5g of sodium chloride, 350ml of tomato extract, 801.5 ml of tween, 1.2g of coix seed powder, 50ml of pork liver extract and 600ml of distilled water;
(2) respectively inoculating Bifidobacterium ovorans and Bifidobacterium longum into a test tube filled with the sterilized culture solution under sterile conditions by using an inoculating ring, and performing shaking culture at 30 ℃ for 25h to respectively obtain Bifidobacterium ovorans culture and Bifidobacterium longum culture;
the preparation method of the intermediate streptococcus culture comprises the following steps:
(1) the formula of the culture solution comprises: 8g of yeast extract, 8g of peptone, 12g of glucose, 150ml of tomato extract, 2g of monopotassium phosphate, 800.5 ml of tween, 1g of silkworm chrysalis shell powder and 850ml of distilled water;
(2) inoculating Streptococcus intermedius into a test tube containing the sterilized culture solution by using an inoculating loop under aseptic conditions, and performing shake culture at 30 ℃ for 25h to obtain Streptococcus intermedius culture;
the preparation method of the propionic acid bacterium freudenreichii culture comprises the following steps:
(1) the formula of the culture solution comprises: 10g of glucose, 20g of sodium lactate, 5g of yeast extract, 0.2g of manganese sulfate, 1g of dipotassium hydrogen phosphate, 0.5g of allium macrostemon powder, 801 ml of tween and 1000ml of distilled water;
(2) inoculating the frey propionic acid bacteria into a test tube containing the sterilized culture solution by using an inoculating loop under the aseptic condition, performing shake culture for 6h at the temperature of 30 ℃, adding sterilized polygonum capitatum powder into the test tube, and continuing to perform shake culture for 15h at the temperature of 25 ℃ to obtain a frey propionic acid bacteria culture;
the preparation method of the aspergillus aconiti acidolyticus culture comprises the following steps:
(1) the formula of the culture solution comprises: 35g of cane sugar, 2g of sodium nitrate, 0.5g of magnesium sulfate, 10g of potato starch, 0.2g of dark plum pulp, 0.01g of ferric sulfate, 1g of monopotassium phosphate, 801 ml of tween and 1000ml of distilled water;
(2) inoculating Aspergillus aconiti acidolyticus into a test tube containing the sterilized culture solution by using an inoculating loop under aseptic condition, performing shake culture at 30 ℃ for 13h, adding sterilized waxberry fruit powder into the test tube, and continuing to perform shake culture at 30 ℃ for 13h to obtain Aspergillus aconiti acidolyticus culture;
the preparation method of the filamentous fungus agent comprises the following steps: adding folium Artemisiae Argyi powder 0.75 wt% into fresh bean dregs, mixing, steaming with strong fire for 305min, cooling, and squeezing to 1cm3And the bean dregs blocks containAnd (3) putting the bean dreg squares on a reticulate pattern air-permeable plate paved with a layer of straw, keeping the temperature at 15 ℃ and the relative humidity at 73%, fermenting for 84h, after the fermentation is finished, picking white fuzz on the bean dreg squares, and performing freeze vacuum drying to obtain the filamentous fungus agent.
3. The special deodorant for large-scale pig farms according to claim 1 or 2, wherein: the using method comprises the following steps: spraying the mixture once every morning and evening in a large-scale pigsty with daily dosage of 0.1-0.2kg/m3。
4. The special deodorant for large-scale pig farms according to claim 1 or 2, wherein: the using method comprises the following steps: spraying the solution once every morning and evening in a large-scale pigsty with a daily dose of 0.15kg/m3。
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511940A (en) * | 2002-12-31 | 2004-07-14 | 福建农林大学 | Compound microbe fermentation strain preparing method and its use |
| CN101711885A (en) * | 2009-12-02 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for preparing high-concentration biological deodorant |
| CN102198284A (en) * | 2011-03-25 | 2011-09-28 | 江苏碧程环保设备有限公司 | Deodorant and preparation method thereof |
| CN104307012A (en) * | 2014-11-05 | 2015-01-28 | 双胞胎（集团）股份有限公司 | Deodorant special for livestock and poultry farms and application thereof |
| EP3015157A1 (en) * | 2014-10-30 | 2016-05-04 | HAGOLA Biofilter GmbH | Filter and filter system |
| CN107149695A (en) * | 2016-03-03 | 2017-09-12 | 上海市农药研究所有限公司 | A kind of complex microorganism deodorant and preparation method and application |
| CN107568071A (en) * | 2017-08-30 | 2018-01-12 | 黄山中科新佳生物科技有限公司 | Biological deodorant for livestock and poultry farm |
-
2018
- 2018-04-04 CN CN201810298012.5A patent/CN108499353B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1511940A (en) * | 2002-12-31 | 2004-07-14 | 福建农林大学 | Compound microbe fermentation strain preparing method and its use |
| CN101711885A (en) * | 2009-12-02 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for preparing high-concentration biological deodorant |
| CN102198284A (en) * | 2011-03-25 | 2011-09-28 | 江苏碧程环保设备有限公司 | Deodorant and preparation method thereof |
| EP3015157A1 (en) * | 2014-10-30 | 2016-05-04 | HAGOLA Biofilter GmbH | Filter and filter system |
| CN104307012A (en) * | 2014-11-05 | 2015-01-28 | 双胞胎（集团）股份有限公司 | Deodorant special for livestock and poultry farms and application thereof |
| CN107149695A (en) * | 2016-03-03 | 2017-09-12 | 上海市农药研究所有限公司 | A kind of complex microorganism deodorant and preparation method and application |
| CN107568071A (en) * | 2017-08-30 | 2018-01-12 | 黄山中科新佳生物科技有限公司 | Biological deodorant for livestock and poultry farm |
Non-Patent Citations (2)
| Title |
|---|
| "提高双歧杆菌活性的研究进展";余倩;《饮料工业》;20111231;17-20 * |
| "除臭微生物分离和筛选方法的改进与应用";陈书安等;《生物技术通报》;20061231;126-129 * |
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Denomination of invention: A specialized deodorant for large-scale pig farms and its application Effective date of registration: 20230626 Granted publication date: 20210101 Pledgee: Wannian Sub branch of Shangrao Bank Co.,Ltd. Pledgor: JIANGXI WANNIAN XINXING AGRICULTURE AND ANIMAL HUSBANDRY CO.,LTD. Registration number: Y2023980045997 |