CN108499353A - A kind of special deodorant in scale pig farm and its application - Google Patents
A kind of special deodorant in scale pig farm and its application Download PDFInfo
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Abstract
The invention discloses a kind of special deodorant in scale pig farm and its applications, it is made of Raman culture of L, oval bifidobacterium culture, bifidobacterium longum culture, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filamentous fungi microbial inoculum, above-mentioned various bacterium cultures and microbial inoculum are added in 6 10% brown sugar aqueous solution, Raman lactobacillus strain concentration is made to reach 105‑107A/ml, oval bifidobacterium species concentration reach 106‑108A/ml, bifidobacterium longum strain concentration reach 104‑106A/ml, intermediary's streptococcus strain concentration reach 107‑109A/ml, Fei Shi bacterium acidi propionici strain concentration reaches 104‑108A/ml, solution aconitic acid aspergillus strain concentration reach 104‑107A/ml, filamentous fungus strain concentration reach 106‑109A/ml.This deodorant handles pig-breeding column home by spray pattern, can the foul gas substance such as fast degradation ammonia, hydrogen sulfide, methane, indoles, volatile fatty acid, mercaptan from the root cause, and will not have an adverse effect to live pig health.
Description
Technical field
The present invention relates to a kind of deodorant, more particularly to the special deodorant in a kind of scale pig farm and its application belong to environment
Protection technique field.
Background technology
Develop as pig-breeding industry surpasses scale direction, the excreta of pig is increasingly tight to the pollution of farm's ambient enviroment
Weight, substantial portion of pig farm gives out very niff, seriously polluted the living environment of surrounding resident.Have at present
160 kinds of volatile ingredients are identified from excrement to be come.More than 80 kinds of nitrogenous compound is also found in fecaluria, wherein having 10 kinds and stench
Taste is related.In addition, fecaluria also will produce ammonia, SO2, NO2, amine and amino acid derivativges etc. during the fermentation.Although ammonia
Between excrement stink it is related less, but numerous studies show that enviromental ammonia is excessively high can influence breeding performonce fo animals and health
Situation, animal feed intake and daily gain decline, and pneumonia incidence rises, and sexal maturity is postponed.
Currently, main method used by processing pig farm foul gas has：Absorption process, absorption method, oxidizing process, combustion method
Deng, but there are treatment effeciency is low, it is inconvenient for operation, and it is also easy to produce secondary pollution problems.Come deodorization it is in recent years by microorganism
The method in the comparison forward position come is broadly divided into regulation and control processing and external regulation and control in animal body and handles two kinds of forms, and regulation and control in vivo are
Microorganism is added in the form of feed addictive in swine rations, pig body is promoted to digest and assimilate nutriment, is reduced smelly
The generation of gas, but there are deodorizing effect unobvious, and there may be antibiotics resistance genes for microorganism, upset the micro- life of intact animal
Object fauna.External regulation and control are that form is concentrated mainly on single deodorization bacteria selection and deodorizing effect detection, and foul gas
Complicated component, effect also unobvious.Therefore a kind of stench of the quick treatment scale pig farm complicated component of energy how is developed
Gas is of great significance for improving breeding efficiency.
Invention content
Present invention aims at a kind of deodorant that efficiently can quickly remove scale pig farm foul gas is provided, can pass through
Sprinkling pig-breeding column home mode is handled, can fast degradation foul gas substance from the root cause, and live pig health will not be produced
Raw adverse effect.
In order to achieve the above object, technical solution provided by the invention is as follows：
A kind of special deodorant in scale pig farm is trained by Raman culture of L, oval bifidobacterium culture, bifidobacterium longum
Support object, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filamentous fungi microbial inoculum composition；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in the brown sugar aqueous solution of 6-10%, make Raman
Lactobacillus strain concentration reaches 105-107A/ml, oval bifidobacterium species concentration reach 106-108A/ml, bifidobacterium longum
Strain concentration reaches 104-106A/ml, intermediary's streptococcus strain concentration reach 107-109A/ml, Fei Shi bacterium acidi propionici strain concentration
Reach 104-108A/ml, solution aconitic acid aspergillus strain concentration reach 104-107A/ml, filamentous fungus strain concentration reach 106-
109A/ml.
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml.
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, its weight is added
30-50 times of water and the complex enzyme of 0.5-0.8% keep 40-50 DEG C of temperature, extract 12-18h, and filter residue is abandoned in filtering, and filtrate is true
Sky dry, pulverize 80 mesh sieve to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:2-3:1-3 is formed；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 12-24h is carried out under the conditions of 25-35 DEG C, obtains Raman culture of L.
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 18-32h is carried out in the test tube of culture solution, under the conditions of 25-35 DEG C, respectively obtains oval bifidobacterium culture and length
Bifidobacterium culture.
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 18-32h is carried out under the conditions of 25-35 DEG C, obtains intermediary's streptococcus culture.
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 4-8h is carried out under the conditions of 25-35 DEG C, and the polygonum capitatum powder of sterilized processing is then added into test tube, keeps temperature
20-30 DEG C continues shaking culture 12-18h, obtains Fei Shi bacterium acidi propionici cultures.
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween-80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 10-16h is carried out under the conditions of 25-35 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps temperature
25-35 DEG C of degree continues shaking culture 10-16h, obtains solution aconitic acid aspergillus culture.
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 0.5-1% is added, is mixed
After uniformly, very hot oven steams 30-40min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then will
Bean dregs square is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 13-16 DEG C of temperature, relative humidity 70-75%, hair
Ferment 72-96h, after fermentation, the white-colored hairs on picking bean dregs square carries out vacuum freezedrying to get filamentous fungi bacterium
Agent.
The application method of the present invention：Respectively sprinkling is primary in the column home of scale pig farm sooner or later daily, and daily dosage is 0.1-
0.2kg/m3。
Advantageous effect
1, this deodorant deodorization speed is fast, significant effect, various bacterium after culture is handled, it is producible abundant beneficial to microbial inoculum and
The enzyme and beneficial products of high activity, while microorganism fungus kind effectively absorbs the active constituent of Chinese herbal medicine etc. in culture solution, is sprayed onto
Scale pig farm column home, ammonia removal rate reaches 80-95% up to 80-95%, hydrogen sulfide removal rate up to 70-85%, methane removal rate in 12h,
Indoles removal rate is up to 80% or more, volatile fatty acid removal rate up to 85% or more, mercaptan removal rate up to 75% or more.
2, by the way that Longstamen Onion Bulb and polygonum capitatum are added in Fei Shi propionic acid bacteria culture fluid and fermentation process, Fei Shi bacterium acidi propionicis can be promoted
More vitamin B12s are generated in incubation, accelerate the decomposition to foul smell.
3, solution aconitic acid aspergillus absorbs nutrition and the active constituent of dark plum pulp and waxberry flesh in preparation process,
It produces the ability of itaconic acid and greatly enhances, and produced itaconic acid can effectively inhibit the growth of putrefactive microorganisms, can fundamentally drop
The generation of low foul gas.
4, filamentous fungi microbial inoculum is in preparation process, the Folium Artemisiae Argyi powder of addition, effectively can carry out purifying culture to filamentous fungi,
The filamentous fungi purity higher of acquisition, while active ingredient in Folium Artemisiae Argyi powder is absorbed, substantially increase its deodorizing effect.
Specific embodiment
Embodiment 1
A kind of special deodorant in scale pig farm is trained by Raman culture of L, oval bifidobacterium culture, bifidobacterium longum
Support object, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filamentous fungi microbial inoculum composition；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in 6% brown sugar aqueous solution, keep Raman newborn
Bacillus species concentration reaches 105A/ml, oval bifidobacterium species concentration reach 106A/ml, bifidobacterium longum strain concentration reach
To 104A/ml, intermediary's streptococcus strain concentration reach 107A/ml, Fei Shi bacterium acidi propionici strain concentration reaches 104A/ml, solution crow
Head love song mould species concentration reaches 104A/ml, filamentous fungus strain concentration reach 106A/ml.
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml.
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, its weight 30 is added
Times water and 0.5% complex enzyme, keep 40 DEG C of temperature, extract 12h, filtering, abandon filter residue, filter vacuum dry, pulverize 80
Mesh sieves to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:2:1 composition；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 25
Shaking culture is carried out under the conditions of DEG C for 24 hours, obtains Raman culture of L.
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 32h is carried out in the test tube of culture solution, under the conditions of 25 DEG C, respectively obtains oval bifidobacterium culture and long bifid bar
Bacterium culture.
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 25
Shaking culture 32h is carried out under the conditions of DEG C, obtains intermediary's streptococcus culture.
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 25
Shaking culture 8h is carried out under the conditions of DEG C, the polygonum capitatum powder of sterilized processing is then added into test tube, keeps 20 DEG C of continuation of temperature
Shaking culture 18h is carried out, Fei Shi bacterium acidi propionici cultures are obtained.
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 16h is carried out under the conditions of 25 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps 25 DEG C of temperature
Continue shaking culture 16h, obtains solution aconitic acid aspergillus culture.
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 0.5% is added, mixing is equal
After even, very hot oven steams 30min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then by bean dregs side
Block is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 13 DEG C of temperature, and relative humidity 70%, ferment 96h, fermentation knot
Shu Hou, the white-colored hairs on picking bean dregs square carry out vacuum freezedrying to get filamentous fungi microbial inoculum.
The application method of the present invention：Respectively sprinkling is primary in the column home of scale pig farm sooner or later daily, and daily dosage is 0.2kg/
m3。
Embodiment 2
A kind of special deodorant in scale pig farm is trained by Raman culture of L, oval bifidobacterium culture, bifidobacterium longum
Support object, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filamentous fungi microbial inoculum composition；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in 8% brown sugar aqueous solution, keep Raman newborn
Bacillus species concentration reaches 106A/ml, oval bifidobacterium species concentration reach 107A/ml, bifidobacterium longum strain concentration reach
To 105A/ml, intermediary's streptococcus strain concentration reach 10/ml, Fei Shi bacterium acidi propionici strain concentration and reach 106A/ml, the solution rhizome of Chinese monkshood
Love song mould species concentration reaches 105A/ml, filamentous fungus strain concentration reach 108A/ml；
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml.
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, its weight 40 is added
Times water and 0.65% complex enzyme, keep temperature 45 C, extract 15h, filtering, abandon filter residue, filter vacuum dry, pulverize
80 mesh sieve to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:2.5:2 compositions；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 18h is carried out under the conditions of DEG C, obtains Raman culture of L.
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 25h is carried out in the test tube of culture solution, under the conditions of 30 DEG C, respectively obtains oval bifidobacterium culture and long bifid bar
Bacterium culture.
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 25h is carried out under the conditions of DEG C, obtains intermediary's streptococcus culture.
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 6h is carried out under the conditions of DEG C, the polygonum capitatum powder of sterilized processing is then added into test tube, keeps 25 DEG C of continuation of temperature
Shaking culture 15h is carried out, Fei Shi bacterium acidi propionici cultures are obtained.
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 13h is carried out under the conditions of 30 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps 30 DEG C of temperature
Continue shaking culture 13h, obtains solution aconitic acid aspergillus culture.
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 0.75% is added, is mixed
After uniformly, very hot oven steams 305min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then by beans
Slag square is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 15 DEG C of temperature, relative humidity 73%, ferment 84h, hair
After ferment, the white-colored hairs on picking bean dregs square carries out vacuum freezedrying to get filamentous fungi microbial inoculum.
The application method of the present invention：Respectively sprinkling is primary in the column home of scale pig farm sooner or later daily, and daily dosage is 0.15kg/
m3。
Embodiment 3
A kind of special deodorant in scale pig farm is trained by Raman culture of L, oval bifidobacterium culture, bifidobacterium longum
Support object, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filamentous fungi microbial inoculum composition；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in 10% brown sugar aqueous solution, keep Raman newborn
Bacillus species concentration reaches 107A/ml, oval bifidobacterium species concentration reach 108A/ml, bifidobacterium longum strain concentration reach
To 106A/ml, intermediary's streptococcus strain concentration reach 109A/ml, Fei Shi bacterium acidi propionici strain concentration reaches 108A/ml, solution crow
Head love song mould species concentration reaches 107A/ml, filamentous fungus strain concentration reach 109A/ml；
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml.
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, its weight 50 is added
Times water and 0.8% complex enzyme, keep temperature 50 C, extract 18h, filtering, abandon filter residue, filter vacuum dry, pulverize 80
Mesh sieves to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:3:3 compositions；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 12h is carried out under the conditions of 35 DEG C, obtains Raman culture of L.
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 18h is carried out in the test tube of culture solution, under the conditions of 35 DEG C, respectively obtains oval bifidobacterium culture and long bifid
Alphacterium culture.
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 18h is carried out under the conditions of 35 DEG C, obtains intermediary's streptococcus culture.
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 35
Shaking culture 4h is carried out under the conditions of DEG C, the polygonum capitatum powder of sterilized processing is then added into test tube, keeps 30 DEG C of continuation of temperature
Shaking culture 12h is carried out, Fei Shi bacterium acidi propionici cultures are obtained.
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 10h is carried out under the conditions of 35 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps 35 DEG C of temperature
Continue shaking culture 10h, obtains solution aconitic acid aspergillus culture.
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 1% is added, is uniformly mixed
Afterwards, very hot oven steams 40min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then by bean dregs square
It is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 16 DEG C of temperature, relative humidity 75%, ferment 72h, fermentation ends
Afterwards, the white-colored hairs on picking bean dregs square carries out vacuum freezedrying to get filamentous fungi microbial inoculum.
The application method of the present invention：Respectively sprinkling is primary in the column home of scale pig farm sooner or later daily, and daily dosage is 0.1kg/
m3。
Comparative example 1：It is a kind of special deodorization of livestock and poultry farm disclosed in CN104307012A according to China Patent Publication No.
The deodorant that agent and its application invention disclosure are prepared, application method are：Sooner or later each in the column home of scale pig farm daily
Sprinkling is primary, and daily dosage is 0.3kg/m3 。
Comparative example 2：It is a kind of microbial deodorant and its system disclosed in CN102247611A according to China Patent Publication No.
The deodorant that Preparation Method disclosure of invention is prepared, application method are：It is respectively sprayed in the column home of scale pig farm sooner or later daily
Once, daily dosage is 0.3kg/m3 。
Test effect：
Sprinkling is implemented respectively in the consistent column home of ten thousand five, pig farm of Nian Xinxing agriculture and animal husbandries limited liability company subordinate scale of Jiangxi
The deodorant of example 1-3 and comparative example 1 and comparative example 2 measure odorous substance degradation situation in 12h, the results showed that：Spray embodiment
Ammonia removal rate reaches 80-95%, indoles removal up to 80-95%, hydrogen sulfide removal rate up to 70-85%, methane removal rate in 1-3 column homes
Rate sprays comparative example 1 and 2 columns up to 80% or more, volatile fatty acid removal rate up to 85% or more, mercaptan removal rate up to 75% or more
House ammonia removal rate be respectively 73% and 75%, hydrogen sulfide removal rate be respectively 45% and 51%, methane removal rate be respectively 68% and
65%, indoles removal rate is respectively that 58% and 65%, volatile fatty acid removal rate is respectively 72% and 66%, mercaptan removal rate difference
For 55% and 60%.Illustrate that deodorant of the present invention can quickly and effectively remove ammonia, hydrogen sulfide, methane, indoles, volatility in swinery house
The odorous gas such as aliphatic acid, mercaptan, significant effect.
Claims (4)
1. a kind of special deodorant in scale pig farm, it is characterised in that：It is cultivated by Raman culture of L, oval Bifidobacterium
Object, bifidobacterium longum culture, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution aconitic acid aspergillus culture, filiform
Fungal inoculant forms；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in the brown sugar aqueous solution of 6-10%, make Raman
Lactobacillus strain concentration reaches 105-107A/ml, oval bifidobacterium species concentration reach 106-108A/ml, bifidobacterium longum
Strain concentration reaches 104-106A/ml, intermediary's streptococcus strain concentration reach 107-109A/ml, Fei Shi bacterium acidi propionici strain concentration
Reach 104-108A/ml, solution aconitic acid aspergillus strain concentration reach 104-107A/ml, filamentous fungus strain concentration reach 106-
109A/ml；
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml；
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, its weight 30-50 is added
The complex enzyme of water again and 0.5-0.8% keeps 40-50 DEG C of temperature, extracts 12-18h, and filtering abandons filter residue, filter vacuum is done
It is dry, 80 mesh sieve is crushed to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:2-3:1-3 is formed；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 12-24h is carried out under the conditions of 25-35 DEG C, obtains Raman culture of L；
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 18-32h is carried out in the test tube of culture solution, under the conditions of 25-35 DEG C, respectively obtains oval bifidobacterium culture and length
Bifidobacterium culture；
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 18-32h is carried out under the conditions of 25-35 DEG C, obtains intermediary's streptococcus culture；
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese,
Shaking culture 4-8h is carried out under the conditions of 25-35 DEG C, and the polygonum capitatum powder of sterilized processing is then added into test tube, keeps temperature
20-30 DEG C continues shaking culture 12-18h, obtains Fei Shi bacterium acidi propionici cultures；
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 10-16h is carried out under the conditions of 25-35 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps temperature
25-35 DEG C of degree continues shaking culture 10-16h, obtains solution aconitic acid aspergillus culture；
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 0.5-1% is added, is uniformly mixed
Afterwards, very hot oven steams 30-40min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then by bean dregs
Square is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 13-16 DEG C of temperature, relative humidity 70-75%, ferment 72-
96h, after fermentation, the white-colored hairs on picking bean dregs square carries out vacuum freezedrying to get filamentous fungi microbial inoculum.
2. a kind of special deodorant in scale pig farm according to claim 1, it is characterised in that：By Raman lactobacillus culture
Object, oval bifidobacterium culture, bifidobacterium longum culture, intermediary's streptococcus culture, Fei Shi bacterium acidi propionicis culture, solution crow
The mould culture of head love song, filamentous fungi microbial inoculum composition；
Preparation method is：Above-mentioned various bacterium cultures and microbial inoculum are added to mixing in 8% brown sugar aqueous solution, keep Raman newborn
Bacillus species concentration reaches 106A/ml, oval bifidobacterium species concentration reach 107A/ml, bifidobacterium longum strain concentration reach
To 105A/ml, intermediary's streptococcus strain concentration reach 10/ml, Fei Shi bacterium acidi propionici strain concentration and reach 106A/ml, the solution rhizome of Chinese monkshood
Love song mould species concentration reaches 105A/ml, filamentous fungus strain concentration reach 108A/ml；
The preparation method of the Raman culture of L：
（1）Culture solution formula composition is：Yeast extract 4.5g, pancreas casein peptone 9.5g, beef extract 9.0g, glucose 18g, citric acid
Diammonium 1.8g, Tween 80 0.8ml, potassium dihydrogen phosphate 1.8g, sodium acetate 4.5g, magnesium sulfate 0.55g, manganese sulfate 0.2g, the rhizome of davallia
Extract 0.2g, distilled water 1000ml；
The preparation method of the Rhizoma Drynariae extract is as follows：The dry rhizome of davallia is taken, 60 mesh sieve is crushed, 40 times of its weight is added
Water and 0.65% complex enzyme, keep temperature 45 C, extract 15h, filtering, abandon filter residue, filter vacuum be dry, pulverize into 80 mesh
Sieve is to get Rhizoma Drynariae extract；
The complex enzyme is by cellulase, pectase, protease according to weight ratio 1:2.5:2 compositions；
（2）Aseptically, Raman lactobacillus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 18h is carried out under the conditions of DEG C, obtains Raman culture of L；
The preparation method of the oval bifidobacterium culture, bifidobacterium longum culture：
（1）Culture solution formula composition is：Peptone 20g, yeast powder 1.5g, glucose 20g, potato starch 0.5g, sodium chloride 5g,
Tomato leachate 350ml, Tween 80 1.5ml, coix seed powder 1.2g, pork liver extracting solution 50ml, distilled water 600ml；
（2）Aseptically, oval Bifidobacterium, bifidobacterium longum are respectively connected to equipped with sterilized above-mentioned with oese
Shaking culture 25h is carried out in the test tube of culture solution, under the conditions of 30 DEG C, respectively obtains oval bifidobacterium culture and long bifid bar
Bacterium culture；
Intermediary's streptococcus culture preparation method is as follows：
（1）Culture solution formula composition is：Yeast extract 8g, peptone 8g, glucose 12g, tomato leachate 150ml, di(2-ethylhexyl)phosphate
Hydrogen potassium 2g, Tween 80 0.5ml, silkworm chrysalis shell powder 1g, distilled water 850ml；
（2）Aseptically, intermediary streptococcus is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 25h is carried out under the conditions of DEG C, obtains intermediary's streptococcus culture；
The preparation method of the Fei Shi bacterium acidi propionicis culture is as follows：
（1）Culture solution formula composition is：Glucose 10g, sodium lactate 20g, yeast extract 5g, manganese sulfate 0.2g, dipotassium hydrogen phosphate 1g,
Macrostem onion powder 0.5g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, Fei Shi bacterium acidi propionicis are accessed in the test tube equipped with sterilized above-mentioned culture solution with oese, 30
Shaking culture 6h is carried out under the conditions of DEG C, the polygonum capitatum powder of sterilized processing is then added into test tube, keeps 25 DEG C of continuation of temperature
Shaking culture 15h is carried out, Fei Shi bacterium acidi propionici cultures are obtained；
The preparation method of the solution aconitic acid aspergillus culture is as follows：
（1）Culture solution formula composition is：Sucrose 35g, sodium nitrate 2g, magnesium sulfate 0.5g, potato starch 10g, dark plum pulp 0.2g,
Ferric sulfate 0.01g, potassium dihydrogen phosphate 1g, Tween 80 1ml, distilled water 1000ml；
（2）Aseptically, it is accessed in the test tube equipped with sterilized above-mentioned culture solution with oese by aconitic acid aspergillus is solved,
Shaking culture 13h is carried out under the conditions of 30 DEG C, and the waxberry flesh powder of sterilized processing is then added into test tube, keeps 30 DEG C of temperature
Continue shaking culture 13h, obtains solution aconitic acid aspergillus culture；
The preparation method of the filamentous fungi microbial inoculum is：New fresh bean dreg is taken, the Folium Artemisiae Argyi powder of its weight 0.75% is added, is uniformly mixed
Afterwards, very hot oven steams 305min, cooling, squeezes into 1cm3Square, and make bean dregs square water content be less than 20%, then by bean dregs side
Block is placed on the reticulate pattern air permeable plate for being laid with one layer of straw, keeps 15 DEG C of temperature, and relative humidity 73%, ferment 84h, fermentation knot
Shu Hou, the white-colored hairs on picking bean dregs square carry out vacuum freezedrying to get filamentous fungi microbial inoculum.
3. a kind of special deodorant in scale pig farm according to claim 1 or 2, it is characterised in that：Its application method：Daily
Sooner or later respectively sprinkling is primary in the column home of scale pig farm, and daily dosage is 0.1-0.2kg/m3。
4. a kind of special deodorant in scale pig farm according to claim 1 or 2, it is characterised in that：Its application method：Daily
Sooner or later respectively sprinkling is primary in the column home of scale pig farm, and daily dosage is 0.15kg/m3。
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Denomination of invention: A specialized deodorant for large-scale pig farms and its application Effective date of registration: 20230626 Granted publication date: 20210101 Pledgee: Wannian Sub branch of Shangrao Bank Co.,Ltd. Pledgor: JIANGXI WANNIAN XINXING AGRICULTURE AND ANIMAL HUSBANDRY CO.,LTD. Registration number: Y2023980045997 |
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