CN107149695A - A kind of complex microorganism deodorant and preparation method and application - Google Patents
A kind of complex microorganism deodorant and preparation method and application Download PDFInfo
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- CN107149695A CN107149695A CN201610119875.2A CN201610119875A CN107149695A CN 107149695 A CN107149695 A CN 107149695A CN 201610119875 A CN201610119875 A CN 201610119875A CN 107149695 A CN107149695 A CN 107149695A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2209/00—Aspects relating to disinfection, sterilisation or deodorisation of air
- A61L2209/20—Method-related aspects
- A61L2209/21—Use of chemical compounds for treating air or the like
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- Botany (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a kind of complex microorganism deodorant, for removing poultry cultivation stink, the deodorant is made up of the material mixing of following percentage by weight：Fungi 1 5%：Including S. cervisiae and/or candidiasis, beneficial bacteria 2 10%：Including streptococcus thermophilus 0 6%, Bifidobacterium 0 6%, bacillus subtilis, bacillus brevis, Bacillus cercus and bacillus megaterium totally 2 10%, carrier 68 97%.The complex microorganism deodorant is used in mixed way with organic materials, is laid in farming site and is carried out deodorization, have the advantages that simple to operate, good deodorization effect, lasting period length, reduce environmental pollution, it is safe to poultry.
Description
Technical field
The present application relates to a kind of complex microorganism deodorant and preparation method and application, belong to microorganism skill
Art field.
Background technology
Odorant species is various, there is different osmophores in different type material molecular structure, thus has not
Same smell and threshold value.In odorant it is larger to human health damage have thio-alcohol, ammonia, hydrogen sulfide,
Dimethyl disulfide, trimethylamine, formaldehyde, styrene, arm acid and phenols etc..Odor pollution main harm human body is exhaled
Desorption system, the circulatory system, digestive system, internal system and nervous system, have had a strong impact on the spirit of people
State, reduces quality of life.
With our people's growth in the living standard, the demand for animal products constantly rises.As China advises
Modelling aquaculture is developed rapidly, and the pollution that a large amount of feces of livestock and poultry of generation are caused turns into China environmental pollution control
The emphasis of system, wherein harm of the foul gas as caused by feces of livestock and poultry not only to livestock and poultry in itself is very big and right
Surrounding enviroment and health also result in grievous injury.Therefore, the exploitation of livestock and poultry cultivation deodorant and it is practical
A focus as research.
Deodorant be broadly divided into physical deodorization agent, chemical deodorizing agent, microbial deodorant, galenical deodorizer and
Composite type deodorization agent etc..Its general principle of microbiocidal deodorant is the stench being dissolved in the water using microorganism
Material absorbing in vivo, makes the process that it is degraded in microorganism itself by the metabolic activity of microorganism.Micro- life
Thing deodorization can be divided into three phases：1. the course of dissolution of foul gas, i.e., be transferred to liquid phase by gas phase；2. it is water-soluble
Odor pollutant is by microorganism adsorption, absorption in liquid；3. the odor pollutant into microbial cell is used as nutriment
Decompose and utilize for Institute of Micro-biology, pollutant is removed.
Microbiocidal deodorant has environmental protection, the repeatable advantage utilized due to it, increasingly by the weight of people
Depending on.But existing microbial deodorant agent producing process is complex, compound bacteria is difficult to obtain, and decomposes poultry and discards
The effect of thing is not good, and smell mouthfeel is not liked causing poultry weight to decline by poultry, and these all limit micro- life
Thing deodorant is further applied.
The content of the invention
The present patent application is that there is provided a kind of new for the above-mentioned weak point present in current microbial deodorant
Type complex microorganism deodorant, the stink substance that the deodorant can be produced during efficient-decomposition livestock culture,
And microorganism species are readily available, preparation technology is simple, can directly apply with livestock and poultry farm, and having
The characteristics of simple and easy to do safety and effectivity.
Livestock and poultry deodorant of the present invention, includes fungi and beneficial bacteria；Wherein fungi (weight containing 1-5%
Percentage, similarly hereinafter), beneficial bacteria contains 2-10%.Fungi includes：S. cervisiae, candidiasis or two
The mixture of person, content is：S. cervisiae 0-5%, candidiasis contain 0-5%；Beneficial bacteria includes：
Bacillus subtilis, streptococcus thermophilus, bacillus brevis, Bifidobacterium, Bacillus cercus, huge gemma
Bacillus or the mixing of above-mentioned multiple beneficial bacterium, content is：Streptococcus thermophilus 0-6%, Bifidobacterium 0-6%,
Bacillus subtilis, bacillus brevis, Bacillus cercus and bacillus megaterium 2-10%.
Present invention also offers the preparation method of complex microorganism deodorant, it is specially：Described fungi and will have
Beneficial bacterium is cultivated respectively, and solid fungicide or liquid bacterial agent is then made.
Wherein, the fluid nutrient medium component of described fungi is：Potato 20%, glucose 1.2g/L,
KH2PO40.1g/L, peptone 0.2g/L, yeast extract 0.15g/L；Add in solid medium liquid medium within
Enter agar 17g/L, pH 6.5.The fluid nutrient medium component of described beneficial bacteria is：Glucose 20g/L, ferment
Female cream 5g/L, peptone 5g/L, beef extract 5g/L, NaCl 5g/L；In solid medium liquid medium within
Add agar 17g/L, pH 6.8-7.2.
After the completion of microculture, the CMC using 0.5% is used as protective agent.Stone flour and/or diatom can be used
Soil makees carrier and makes solid fungicide, or liquid bacterial agent is made.
Present invention also offers complex microorganism deodorant application process, available in livestock and poultry cultivation.Specially：
It is laid in after the solid fungicide being made or liquid bacterial agent are mixed with organic materials in livestock and poultry cultivation place, organic matter
Material can be the mixing of any one or more in rice husk, sawdust Huo Ku stalks.
Complex microorganism of the present invention is used for chicken farm, and tiling is organically combined with raising chickens in place with chicken manure,
Deodorizing effect is obvious, and applied once, and 2-3 months effectively.
The microbial culture method of complex microorganism deodorant of the present invention is：
(1) above-mentioned correspondence 50ml nutrient solutions are formulated into respectively, and fungi group and bacterial flora are respectively pivoted to 250ml tri-
In the flask of angle, after conventional autoclaving, the microorganism fungus kind of solid medium culture is inoculated with respectively.Connect
Conical flask after kind is put in shaking table shaken cultivation, and the condition of culture of fungi group is：Shaking speed 210rpm,
24 DEG C of temperature, time 96h；The condition of culture of bacterial flora is 28-36 DEG C, 48-72h, and shaking speed is according to bacterium
Strain requires different and adjusted.
(2) bacterial fermentation tank culture：The same Shaking culture of early stage culture, takes out after 24 hours Shaking cultures, respectively
Three parts, i.e. bacillus subtilis, bacillus brevis, Bacillus cercus and bacillus megaterium is merged into close
Together, streptococcus thermophilus and Bifidobacterium are individually cultivated, and are cultivated respectively in fermentation tank, inoculum concentration 5%,
Throughput is adjusted according to bacterial strain to api request, cultivation temperature is 28-36 DEG C, incubation time 48-72h.
Speed of agitator is adjusted also according to the requirement for cultivating different bacterium.
The preparation of complex micro organism fungicide of the present invention and store method：Protected using 0.5% CMC
Agent, solid fungicide is made using talcum powder and/or diatomite as carrier, or liquid bacterial agent is made, and is positioned over room
Temperature is lower to be preserved.
The application of complex microorganism deodorant of the present invention：Complex micro organism fungicide is made according to the method described above
Mixed with organic materials, mixed proportion is：1: 99 (weight ratio), and stir.Then every square is pressed
Meter Hou Du is 10-30 centimetres) ground is laid in livestock culture room.
Complex microorganism deodorant of the present invention is applied during poultry, through experiment, with blank control group
Compare, the stink that the excreta such as fecaluria for producing is brought during poultry can be greatly reduced, with prior art phase
Than, the beneficial effects of the invention are as follows：1) directly it can be contacted with livestock and poultry, during livestock and poultry farm,
It is nontoxic to fowl poultry safety；2) easy to operate, applied once can be maintained 2-3 months；3) good deodorization effect is smelly
Gas concentration is substantially reduced.
Embodiment
With reference to instantiation, the present invention is expanded on further.It should be understood that these embodiments are only used for this hair
It is bright to illustrate, and be not construed as limiting the scope of claims limitation, it may occur to persons skilled in the art that other
Alternative means, within invention right.
Strain and other kinds test material involved by following examples, are to be obtained by commercially available.
The fermented and cultured of the complex microorganism of embodiment 1
1) the fluid nutrient medium component of fungi is：Potato 20%, glucose 1.2g/L, KH2PO40.1g/L、
Peptone 0.2g/L, yeast extract 0.15g/L；Agar 17g/L is added in solid medium liquid medium within,
pH 6.5。
2) the fluid nutrient medium component of beneficial bacteria is：Glucose 20g/L, yeast extract 5g/L, peptone 5g/L,
Beef extract 5g/L, NaCl 5g/L；Agar 17g/L, pH 6.8-7.2 are added in solid medium liquid medium within.
3) 50ml fungus culture mediums are prepared according to the above method, be attached in 250ml conical flasks, solid training is made
Base is supported, after conventional autoclaving, S. cervisiae and candidiasis are inoculated into solid culture respectively
In base.Conical flask after inoculation is put in shaking table shaken cultivation, and the condition of culture of fungi group is：Shaking speed
210rpm, 24 DEG C of temperature, time 96h；
4) 50ml beneficial bacteria nutrient solutions are prepared according to the above method, be attached in 250ml conical flasks, is made solid
Body culture medium, after conventional autoclaving, by bacillus subtilis, streptococcus thermophilus, bacillus brevis,
Bifidobacterium, Bacillus cercus, bacillus megaterium are inoculated into solid medium respectively.Three after inoculation
Angle flask is put in shaking table shaken cultivation, and the condition of culture of bacterial flora is 28-36 DEG C, 48-72h, shaking speed root
Require different according to bacterial strain and adjust.
5) bacterial fermentation tank culture：The same Shaking culture of early stage culture, takes out after 24 hours Shaking cultures, closes respectively
And closed into three parts, i.e. bacillus subtilis, bacillus brevis, Bacillus cercus and bacillus megaterium
Together, streptococcus thermophilus and Bifidobacterium are individually cultivated, and are cultivated respectively in fermentation tank, inoculum concentration 5%, logical
Tolerance is adjusted according to bacterial strain to api request, cultivation temperature is 28-36 DEG C, incubation time 48-72h.Stir
Mix rotating speed is adjusted also according to the requirement for cultivating different bacterium.
The preparation of the complex micro organism fungicide of embodiment 2
1) preparation of solid fungicide：By fungus culture medium made from embodiment 1 and beneficial bacteria nutrient solution according to
Solid bacterium is made after being well mixed and dry with carrier (talcum powder and/or diatomite) in part by weight described in table 1
Agent：
The weight percentages of components (%) of the solid fungicide of table 1
2) preparation of liquid bacterial agent：By fungus culture medium made from embodiment 1 and beneficial bacteria nutrient solution according to
Part by weight described in table 2 adds 1% (weight ratio) protective agent (0.5% carboxymethyl after being well mixed with carrier
Cellulose), liquid bacterial agent is made：
The weight percentages of components (%) of the liquid bacterial agent of table 2
The measure of the complex micro organism fungicide of embodiment 3
Solid made from embodiment 2 and 3 and liquid bacterial agent are placed and preserved at room temperature, respectively at the 2nd, 4,
6th, 8, weigh microbial inoculum within 10 months to be put in sterilized water, by gradient dilution, be coated on solid medium, and
And the work thalline quantity in its different storage time section preparation is detected, it is repeated 3 times.Testing result is shown in Table 3：
The complex micro organism fungicide viable count testing result of table 3
Deodorization application of the complex micro organism fungicide of embodiment 4 during poultry
100 square metres of indoor culture places of hidding and sunny side are chosen, the cut-off that centre is made with color steel is divided into
The closed space relatively of four of each 25 square metres, including three test groups and a control group.First will
Rice husk (has preserved 10 with #4 (Fresh), the #4 in the table 1 of embodiment 1 respectively as organic materials
Individual month) and #6 (Fresh) according to 10：1 part by weight is mixed, and be laid on respectively code name for I, II,
In 25 square meter places of III test group, laying depth is 15 centimetres, and each test group delivers 100 respectively
Plumage reproduction age is the black chicken of new poplar of 20 days, is fed 60 days with normal condition.In feeding 20 days, 40 days and 60
It when, observation chicken group's growing state finds that the chicken group of three test groups has no adverse reaction, well-grown.Simultaneously
The air in three test groups and control group space is sampled respectively, first three day of sampling is kept in four spaces
It is closed until sampling terminate.Foul smell is determined using national standard GB/T 14675-93 triangle odor bag method dense
Degree, detection method is that with three odorlesses bag, 2 therein are filled with no odour, and another is by certain thinner ratio
Example be filled with odorless air and detection sample for it is several smell the person of distinguishing and smell distinguish, after smelling the person of distinguishing correctly identification have foul smell bag,
Diluting step by step, smelling and distinguish, until stopping smelling when the odor concentration of dilute sample is less than the odor threshold for smelling the person of distinguishing distinguishing.
Finally, the concentration that the person of distinguishing smells the average dilution multiple, as foul smell of distinguishing dilute sample is smelt by several.
Measurement result is shown in Table 4.
Foul smell measurement result during the poultry of table 4
Note：It is diluted, is diluted to just with odorless air 1. odor concentration refers to foul gas (including peculiar smell)
Odorless is required extension rate, dimensionless.
2. it is the volunteer by olfactometry to smell the person of distinguishing.
3. growing state well refers to that survival rate, average weight and the eurhythmia degree level of chicken group is above
Control group.
According to the odor concentration testing result of table 4, show that complex microorganism deodorant of the present invention can be effective
The stink substance produced during reduction poultry, is effectively controlled using rear odor concentration, complies fully with country
On the odorant pollutant row described in livestock and poultry breeding industry emission standard for odor pollutants (GB 18596-2001)
Put standard, and there is easy to use, lasting period length, it is safe to birds.
Claims (13)
1. a kind of complex microorganism deodorant, it is characterised in that described deodorant includes fungi and beneficial bacteria；
Wherein fungi contains 1-5%, and beneficial bacteria contains 2-10%.
2. deodorant as claimed in claim 1, it is characterised in that described fungi includes：S. cervisiae,
Candidiasis or the mixture of the two；Described beneficial bacteria includes：Bacillus subtilis, streptococcus thermophilus,
Bacillus brevis, Bifidobacterium, Bacillus cercus, bacillus megaterium or above-mentioned multiple beneficial bacterium it is mixed
Close.
3. the deodorant as described in claim any one of 1-2, it is characterised in that the content of the microorganism is：
S. cervisiae 0-5%, candidiasis 0-5%, streptococcus thermophilus 0-6%, Bifidobacterium 0-6%, withered grass
Bacillus, bacillus brevis, Bacillus cercus and bacillus megaterium 2-10%.
4. the deodorant as described in claim any one of 1-2, it is characterised in that described deodorant uses 0.5%
Carboxymethyl cellulose liquid bacterial agent is made as protective agent.
5. the deodorant as described in claim any one of 1-2, it is characterised in that described deodorant uses 0.5%
Carboxymethyl cellulose make protective agent, add stone flour and/or solid fungicide be made as carrier in diatomite.
6. a kind of preparation method of complex microorganism deodorant, it is characterised in that by described fungi and beneficial bacteria
Cultivate respectively, solid fungicide or liquid bacterial agent is then made.
7. preparation method as claimed in claim 6, it is characterised in that the fluid nutrient medium component of described fungi
For：Potato 20%, glucose 1.2g/L, KH2PO40.1g/L, peptone 0.2g/L, yeast extract 0.15g/L；
The solid medium of described fungi is that agar 17g/L, pH 6.5 is added in described fluid nutrient medium.
8. preparation method as claimed in claim 6, it is characterised in that the fluid nutrient medium of described beneficial bacteria
Component is glucose 20g/L, yeast extract 5g/L, peptone 5g/L, beef extract 5g/L, NaCl 5g/L；Institute
The solid medium for stating beneficial bacteria is that agar 17g/L, pH 6.8-7.2 are added in described fluid nutrient medium.
9. preparation method as claimed in claim 6, it is characterised in that stone flour and/or silicon can be used in the deodorant
Diatomaceous earth makees carrier and makes solid fungicide, or liquid bacterial agent is made.
10. application of the deodorant as described in claim any one of 1-5 in livestock culture.
11. application as claimed in claim 10, it is characterised in that for the deodorization during poultry.
12. the application as described in claim 10 or 11, it is characterised in that in use by deodorant and organic matter
Material mixing, and in tiling and farming site.
13. application as claimed in claim 12, it is characterised in that described organic materials includes rice husk, sawdust
The mixing of any one or more in Huo Ku stalks.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520628A (en) * | 2016-12-11 | 2017-03-22 | 钦州市康绿宝农业有限公司 | Microbial deodorant and preparation method thereof |
| CN107638795A (en) * | 2017-10-18 | 2018-01-30 | 贵州德莱易生物科技有限公司 | One kind de-tastes degerming spray |
| CN108499353A (en) * | 2018-04-04 | 2018-09-07 | 江西万年鑫星农牧股份有限公司 | A kind of special deodorant in scale pig farm and its application |
| CN109485215A (en) * | 2018-12-26 | 2019-03-19 | 桂林理工大学 | A method of municipal sludge stink is removed by major auxiliary burden of Radix Glycyrrhizae |
| CN109609401A (en) * | 2018-12-18 | 2019-04-12 | 山东省科学院生态研究所 | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof |
| CN110150505A (en) * | 2019-06-21 | 2019-08-23 | 苏州宏螯生物农业发展有限公司 | A kind of lactic acid bacteria complexing agent and preparation method thereof removing Eriocheir sinensis bilgy odour |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520628A (en) * | 2016-12-11 | 2017-03-22 | 钦州市康绿宝农业有限公司 | Microbial deodorant and preparation method thereof |
| CN107638795A (en) * | 2017-10-18 | 2018-01-30 | 贵州德莱易生物科技有限公司 | One kind de-tastes degerming spray |
| CN108499353A (en) * | 2018-04-04 | 2018-09-07 | 江西万年鑫星农牧股份有限公司 | A kind of special deodorant in scale pig farm and its application |
| CN108499353B (en) * | 2018-04-04 | 2021-01-01 | 江西万年鑫星农牧股份有限公司 | Special deodorant for large-scale pig farm and application of special deodorant |
| CN109609401A (en) * | 2018-12-18 | 2019-04-12 | 山东省科学院生态研究所 | A kind of complex microorganism deodorizing microorganism and the preparation method and application thereof |
| CN109609401B (en) * | 2018-12-18 | 2020-05-12 | 山东省科学院生态研究所 | Compound microbial deodorant bacterial agent and preparation method and application thereof |
| CN109485215A (en) * | 2018-12-26 | 2019-03-19 | 桂林理工大学 | A method of municipal sludge stink is removed by major auxiliary burden of Radix Glycyrrhizae |
| CN110150505A (en) * | 2019-06-21 | 2019-08-23 | 苏州宏螯生物农业发展有限公司 | A kind of lactic acid bacteria complexing agent and preparation method thereof removing Eriocheir sinensis bilgy odour |
| CN110150505B (en) * | 2019-06-21 | 2022-09-27 | 苏州宏螯生物农业发展有限公司 | Lactic acid bacteria complexing agent for removing earthy smell of eriocheir sinensis and preparation method thereof |
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