CN104307014A - Microbial deodorant and preparation method thereof - Google Patents
Microbial deodorant and preparation method thereof Download PDFInfo
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- CN104307014A CN104307014A CN201410498931.9A CN201410498931A CN104307014A CN 104307014 A CN104307014 A CN 104307014A CN 201410498931 A CN201410498931 A CN 201410498931A CN 104307014 A CN104307014 A CN 104307014A
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Abstract
A microbial deodorant and its preparation method. The invention discloses a microbial deodorant which is prepared by the following steps: mixing 60-90 wt% of a lactobacillus broth, 1-10 wt% of a bacillus broth and 9-30 wt% of a microzyme broth, inoculating the mixture to a soybean yellow serofluid, standing at 33-40 DEG C, sealing and culturing for 24-72 hours. The above microbial deodorant is obtained by inoculating common environmentally-friendly microbes to low-cost soybean yellow serofluid and culturing. Costs are reduced, and the microbial deodorant is green and pollution-free. The microbial deodorant has a good effect of removing main fetid components. Average removal rate of NH3 reaches more than 90%, and average removal rate of H2S reaches more than 70%. Thus, the above microbial deodorant can widely be used in deodorization and purification of the air at places such as rubbish, sewage, livestock and animal houses, food processing plants and the like.
Description
Technical field
The present invention relates to environmental protection and microorganism field, particularly relate to microbial deodorant and preparation method thereof.
Background technology
In Urbanization in China process, urban population is increasing, incident be Domestic Refuse tell increase.According to incompletely statistics, China's rubbish rate of increase reaches more than 10% in recent years, annual nearly 1.5 hundred million tons of generation.At present, domestic city house refuse burden is 7,000,000,000 tons, and take up an area about more than 80 ten thousand mu, wherein 80-90% is from big and medium-sized cities, and the quantity of refuse on average produced for each person every day is 1.2 kilograms, but its treating capacity is only about 50%.More than 600 city, the whole nation, the big and medium-sized cities of existing 2/3rds are absorbed in the predicament of " garbage-surrounded city ", create " foul smell disturbs residents " problem be on the rise thereupon.
For the problems referred to above, the existing microbial deodorant of existing market mainly comprises chemical deodorizing agent, plant deodorant and microbial deodorant.Chemical deodorizing agent cost is higher, cause secondary pollution; Plant deodorant extraction process is complicated, production cost is higher; And microbial deodorant becomes the focus of field of deodorization now because of its " good and cheap ".
Yellow seriflux solidifies at hot soya-bean milk or produces the liquid being squeezed out in the process of bean curd, because its color belt is yellow, therefore is commonly called as in industry as yellow seriflux.In general solid content more than 1% in yellow seriflux, wherein protein content about 0.3%, fat content about 0.09%, content of reducing sugar is about 0.15%.Simultaneously also containing multiple biological active substances health being had to specific function, as soybean whey protein, soybean oligo saccharide, soybean isoflavone, soybean saponin etc.But yellow seriflux directly discharges by most bean product enterprise of China, does not only make full use of its nutritive value, and direct exhaust emission environment, be unfavorable for cleaner production and increase soybean processing added value.
Current microbial deodorant product or technology more, yeast as Guangdong Microbes Inst application is main deodorization technology (application number 02152024.0 and application number 01129774.3), and China Agricultural University's application with non-bacillus cereus composite function bacillus cereus deodorization technologies (application number 200510069515.8) such as yeast, lactobacillus, nitrogen-fixing bacteria and photosynthetic bacterias.And not about utilizing yellow seriflux to carry out fermentation for microbial deodorant.Therefore, utilize fermentable yellow plasm of soybean to prepare microbial deodorant and filled up domestic blank, its market also has broad prospects.
Summary of the invention
Based on this, be necessary to provide a kind of to microbial deodorant that yellow plasm of soybean ferments and preparation method thereof.
A kind of microbial deodorant, described deodorizer is after adopting the yeast fermentation liquor of the streptococcus acidi lactici fermented solution of 60%-90% weight ratio, the fermentation of bacillus liquid of 1%-10% weight ratio and 9%-30% weight ratio to mix, be seeded in yellow plasm of soybean, at 33-40 DEG C, then leave standstill sealing cultivate and prepare after 24-72 hour.
Wherein in an embodiment, the effective bacterium total bacteria count in described deodorizer is no less than 1 × 10
8cfu/mL, the quantity of the effective bacterium in described streptococcus acidi lactici fermented solution, fermentation of bacillus liquid and yeast fermentation liquor is all no less than 1 × 10
6cfu/mL.
Wherein in an embodiment, the percentage composition of the sugar in described yellow seriflux is 0-10%, and described sugar is glucose, white sugar, brown sugar or molasses.
Wherein in an embodiment, described lactobacillus is Lactobacillus, Leuconostoc, Bifidobacterium or Pediococcus.
Wherein in an embodiment, described bacillus cereus is bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus cereus or Brevibacillus brevis.
Wherein in an embodiment, described yeast is saccharomyces cerevisiae, Saccharomyces uvarum, Hansenula yeast, torulopsis, candida mycoderma, Rhodothece glutinis or geotrichum candidum.
The preparation method of mentioned microorganism deodorizer, comprises the following steps:
The yeast fermentation liquor mixing of the fermentation of bacillus liquid of the streptococcus acidi lactici fermented solution of 60%-90% weight ratio, 1%-10% weight ratio and 9-30% weight ratio is mixed, obtains mixed culture fermentation liquid;
Described mixed culture fermentation liquid is inoculated in yellow plasm of soybean, at 33-40 DEG C, leaves standstill sealing cultivate 24-72 hour, obtain microbial deodorant.
Wherein in an embodiment, described streptococcus acidi lactici fermented solution is prepared by following methods:
By lactobacillus inoculum in lactic acid bacteria culturing medium, at 33-40 DEG C, quiescent culture 24-72 hour, obtains streptococcus acidi lactici fermented solution;
Described lactic acid bacteria culturing medium, percentage, comprises following component:
Peptone 1%, yeast extract 0.5%, sodium acetate 0.5%, magnesium sulfate 0.05%, manganese sulfate 0.025%, potassium dihydrogen phosphate 0.2% and glucose 1.5%, surplus is water.
Wherein in an embodiment, described fermentation of bacillus liquid is prepared by following methods:
Be seeded to by bacillus cereus in bacillus culture medium, at 25-35 DEG C, aerobic cultivation 36-48 hour, obtains fermentation of bacillus liquid;
Described bacillus culture medium, percentage, comprises following component:
Tryptone 1%, yeast extract 0.5%, sodium chloride 1%, surplus is water.
Wherein in an embodiment, being prepared by following methods of described yeast fermentation liquor:
Be seeded to by yeast in microzyme culture medium, at 25-35 DEG C, aerobic cultivation 24-48 hour, obtains yeast fermentation liquor;
Described microzyme culture medium, percentage, comprises following component:
Yeast extract 1%, peptone 2% and glucose 2%, surplus is water.
Mentioned microorganism deodorizer, by common and eco-friendly microorganism, is seeded in yellow plasm of soybean with low cost to cultivate and obtains, reduce cost and green non-pollution.The main component of this microbial deodorant to stench has good removal effect, NH
3average removal rate be more than 90%, H
2the average removal rate of S is more than 70%.Therefore, mentioned microorganism deodorizer can be widely used in deodorization and the purification of the air in the place such as rubbish, sewage, livestock and poultry animal house institute, food processing factory.
Detailed description of the invention
For enabling above-mentioned purpose of the present invention, feature and advantage become apparent more, are described in detail below to the specific embodiment of the present invention.Set forth a lot of detail in the following description so that fully understand the present invention.But the present invention can be much different from alternate manner described here to implement, those skilled in the art can when without prejudice to doing similar improvement when intension of the present invention, therefore the present invention is by the restriction of following public concrete enforcement.
The microbial deodorant of one embodiment, this deodorizer is after the yeast fermentation liquor mixing adopting the streptococcus acidi lactici fermented solution of 60%-90% weight ratio, the fermentation of bacillus liquid of 1%-10% weight by weight part and 9%-30% weight ratio part, be seeded in yellow plasm of soybean, at 33-40 DEG C, then leave standstill sealing cultivate and prepare after 24-72 hour.In the present embodiment, lactobacillus is Lactobacillus, Leuconostoc, Bifidobacterium or Pediococcus.Bacillus cereus is bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus cereus or Brevibacillus brevis.Yeast is saccharomyces cerevisiae, Saccharomyces uvarum, Hansenula yeast, torulopsis, candida mycoderma, Rhodothece glutinis or geotrichum candidum.
Wherein, the effective bacterium total bacteria count in deodorizer is no less than 1 × 10
8cfu/mL, the quantity of the effective bacterium in described streptococcus acidi lactici fermented solution, fermentation of bacillus liquid and yeast fermentation liquor is all no less than 1 × 10
6cfu/mL, the quantity of the effective bacterium namely in often kind of fermentation liquid is all no less than 1 × 10
6cfu/mL.
With specific embodiment, the present invention is further elaborated below.
Strain used in following examples is all bought in China General Microbiological culture presevation administrative center.
Embodiment 1
A kind of microbial deodorant, it is prepared by following methods:
Under sterile working, in preservation inclined-plane, difference picking one bacillus acidophilus (CICC 6088), bacillus subtilis (ACCC11062) and saccharomyces cerevisiae (CICC31105) are seeded in seed culture medium and cultivate.Wherein, the seed culture medium of lactobacterium acidophilus is peptone 1%, yeast extract 0.5%, sodium acetate 0.5%, magnesium sulfate 0.05%, manganese sulfate 0.025%, potassium dihydrogen phosphate 0.2% and glucose 1.5%.Quiescent culture 24-72 hour at 33-40 DEG C; The seed culture medium of bacillus subtilis is tryptone 1%, yeast extract 0.5%, sodium chloride 1%.Aerobic cultivation 36-48 hour at 25-35 DEG C; The seed culture medium of saccharomyces cerevisiae is yeast extract 1%, peptone 2% and glucose 2%.Aerobic cultivation 24-48 hour at 25-35 DEG C.
By bacillus acidophilus, bacillus subtilis and saccharomyces cerevisiae according to 85%, 5% and 10% weight ratio be mixed into seed liquor, access in 9L yellow seriflux with 10% of seed liquor and yellow seriflux gross weight again and (add brown sugar or the molasses of 1% gross weight outward), leave standstill sealing at 33-40 DEG C and cultivate 24-72 hour.Adopt gradient dilution to be coated with total bacteria count in Plating detection fermentation liquid and reach 8 × 10
8namely cfu/ml obtains microbial deodorant.
Embodiment 2
A kind of microbial deodorant, the difference of preparation method and embodiment 1 is, the weight ratio of bacillus acidophilus, bacillus subtilis and saccharomyces cerevisiae is respectively 60%, 10% and 30%, and all the other steps are with embodiment 1.
Embodiment 3
A kind of microbial deodorant, the difference of its preparation method and embodiment 1 is, the weight ratio of bacillus acidophilus, bacillus subtilis and saccharomyces cerevisiae is respectively 90%, 1% and 9%, and all the other steps are with embodiment 1.
Embodiment 4
1, indoor refuse deodorizing test
Stack in confined chamber and highly rot and produce the house refuse of stench, the microbial deodorant tap water of embodiment 1 is diluted 10 times (volume ratios), evenly sprays 100mL-200mL with spray gun.After 20 minutes around rubbish different acquisition ground point sampling, detect ammonia, sulfureted hydrogen gas concentration, contrast spray before and after ammonia and hydrogen sulfide removal rate.Sampled point is located at from 1m place, edge, smelly source, and height 1.5m, 5 point samplings, average.Odor concentration adopts triangle odor bag method (GB/T14675), and ammonia adopts sodium hypochlorite-salicylic acid spectrophotography (GB/T14679), and hydrogen sulfide adopts ammonium alcohol polyvinyl phosphate absorption-methylene-blue colorimetric method (GB/T16489).Testing result is as shown in table 1:
The microbial deodorant lab simulation deodorizing effect result of the test of table 1 embodiment 1
Note: deodorization concentration unit, dimensionless.
As shown in Table 1, adopt the microbial deodorant of embodiment 1, indoor deodorizing effect is obvious, and wherein, the average removal rate of ammonia reaches 95.09%, and the average removal rate of hydrogen sulfide reaches 84.34%, and the average removal rate of foul smell reaches 90.63%.
2, microbial deodorant application test
Select a garbage compression station in Changsha community, be sprayed on compressor station with the microbial deodorant of 1L embodiment 1, detect ammonia concentration, concentration of hydrogen sulfide and odor concentration after 1 hour before spraying and after sandblasting, testing result is as shown in table 2:
The deodorizing effect of the microbial deodorant of table 2 embodiment 1
| Project | Before sprinkling | After sprinkling | Reduction rate (%) |
| Ammonia (mg/m 3） | 1.372 | 0.067 | 95.12 |
| Hydrogen sulfide (mg/m 3） | 0.104 | 0.032 | 69.23 |
| Foul smell | 1034.3 | 103.9 | 89.95 |
As shown in Table 2, the microbial deodorant effect of embodiment 1 was sprayed after 1 hour in household garbage-compressing station, odor concentration, ammonia concentration and concentration of hydrogen sulfide all obviously reduce than before sprinkling, wherein, ammonia concentration reduces 95.12%, concentration of hydrogen sulfide reduces 69.23%, and odor concentration reduces 89.95%.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a microbial deodorant, it is characterized in that, described deodorizer is after adopting the yeast fermentation liquor of the streptococcus acidi lactici fermented solution of 60%-90% weight ratio, the fermentation of bacillus liquid of 1%-10% weight ratio and 9%-30% weight ratio to mix, be seeded in yellow plasm of soybean, at 33-40 DEG C, then leave standstill sealing cultivate and prepare after 24-72 hour.
2. biological deodorant according to claim 1, is characterized in that, the effective bacterium total bacteria count in described deodorizer is no less than 1 × 10
8cfu/mL, the quantity of the effective bacterium in described streptococcus acidi lactici fermented solution, fermentation of bacillus liquid and yeast fermentation liquor is all no less than 1 × 10
6cfu/mL.
3. biological deodorant according to claim 1, is characterized in that, the percentage composition of the sugar in described yellow seriflux is 0-10%, and described sugar is glucose, white sugar, brown sugar or molasses.
4. biological deodorant according to claim 1, is characterized in that, described lactobacillus is Lactobacillus, Leuconostoc, Bifidobacterium or Pediococcus.
5. biological deodorant according to claim 1, is characterized in that, described bacillus cereus is bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus cereus or Brevibacillus brevis.
6. the biological deodorant according to any one of claim 1-5, is characterized in that, described yeast is saccharomyces cerevisiae, Saccharomyces uvarum, Hansenula yeast, torulopsis, candida mycoderma, Rhodothece glutinis or geotrichum candidum.
7. a preparation method for the microbial deodorant described in any one of claim 1-6, is characterized in that, comprises the following steps:
The yeast fermentation liquor of the fermentation of bacillus liquid of the streptococcus acidi lactici fermented solution of 60%-90% weight ratio, 1%-10% weight ratio and 9%-30% weight ratio is mixed, obtains mixed culture fermentation liquid;
Described mixed culture fermentation liquid is inoculated in yellow plasm of soybean, at 33-40 DEG C, leaves standstill sealing cultivate 24-72 hour, obtain microbial deodorant.
8. the preparation method of microbial deodorant according to claim 7, is characterized in that, described streptococcus acidi lactici fermented solution is prepared by following methods:
By lactobacillus inoculum in lactic acid bacteria culturing medium, at 33-40 DEG C, quiescent culture 24-72 hour, obtains streptococcus acidi lactici fermented solution;
Described lactic acid bacteria culturing medium, percentage, comprises following component:
Peptone 1%, yeast extract 0.5%, sodium acetate 0.5%, magnesium sulfate 0.05%, manganese sulfate 0.025%, potassium dihydrogen phosphate 0.2% and glucose 1.5%, surplus is water.
9. the preparation method of microbial deodorant according to claim 7, is characterized in that, described fermentation of bacillus liquid is prepared by following methods:
Be seeded to by bacillus cereus in bacillus culture medium, at 25-35 DEG C, aerobic cultivation 36-48 hour, obtains fermentation of bacillus liquid;
Described bacillus culture medium, percentage, comprises following component:
Tryptone 1%, yeast extract 0.5%, sodium chloride 1%, surplus is water.
10. the preparation method of the microbial deodorant according to any one of claim 7-9, is characterized in that, being prepared by following methods of described yeast fermentation liquor:
Be seeded to by yeast in microzyme culture medium, at 25-35 DEG C, aerobic cultivation 24-48 hour, obtains yeast fermentation liquor;
Described microzyme culture medium, percentage, comprises following component:
Yeast extract 1%, peptone 2% and glucose 2%, surplus is water.
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| CN104789491A (en) * | 2015-03-24 | 2015-07-22 | 福建省农业科学院农业工程技术研究所 | Bacillus licheniformis strain and application thereof |
| CN104878060A (en) * | 2015-06-25 | 2015-09-02 | 湖南农业大学 | Bacillus subtilis culture medium for producing anti-microbial peptide and application thereof |
| CN106520597A (en) * | 2016-10-18 | 2017-03-22 | 河南省奶牛生产性能测定中心 | Ecological activity deodorant and preparation method thereof |
| CN106520628A (en) * | 2016-12-11 | 2017-03-22 | 钦州市康绿宝农业有限公司 | Microbial deodorant and preparation method thereof |
| CN106902631A (en) * | 2017-02-23 | 2017-06-30 | 浙江加百列生物科技有限公司 | A kind of microbial deodorant and production technology |
| CN107058174A (en) * | 2017-03-03 | 2017-08-18 | 神奇国际投资控股有限公司 | A kind of preparation method of efficient deodorizing functional microorganism ferment mould liquid |
| CN107149695A (en) * | 2016-03-03 | 2017-09-12 | 上海市农药研究所有限公司 | A kind of complex microorganism deodorant and preparation method and application |
| CN107189972A (en) * | 2017-07-27 | 2017-09-22 | 蓝德环保科技集团股份有限公司 | Microbial deodorant, preparation method and the usage |
| CN108097032A (en) * | 2017-12-29 | 2018-06-01 | 广州广牧丰生物技术有限公司 | A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant |
| CN108217971A (en) * | 2017-12-08 | 2018-06-29 | 广州资源环保科技股份有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN108271785A (en) * | 2018-01-30 | 2018-07-13 | 南京工业大学 | A kind of biological pesticide and preparation method thereof using the production of bean curd yellow water by fermentation |
| CN109351175A (en) * | 2018-10-31 | 2019-02-19 | 北京盛源佳业环保科技有限公司 | A kind of quick-acting microbial deodorants |
| KR101993762B1 (en) * | 2018-08-07 | 2019-07-01 | 주식회사 건영이앤씨 | Liquefied deodorant and fabricating method thereof |
| CN112295401A (en) * | 2020-12-29 | 2021-02-02 | 南京凯创协同纳米技术有限公司 | Preparation method of spray for removing fishy smell of farmer market |
| CN113980840A (en) * | 2021-10-15 | 2022-01-28 | 辽宁农业职业技术学院 | Microbial deodorant liquid for public environment and preparation method thereof |
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| CN104789491A (en) * | 2015-03-24 | 2015-07-22 | 福建省农业科学院农业工程技术研究所 | Bacillus licheniformis strain and application thereof |
| CN104878060A (en) * | 2015-06-25 | 2015-09-02 | 湖南农业大学 | Bacillus subtilis culture medium for producing anti-microbial peptide and application thereof |
| CN107149695A (en) * | 2016-03-03 | 2017-09-12 | 上海市农药研究所有限公司 | A kind of complex microorganism deodorant and preparation method and application |
| CN106520597A (en) * | 2016-10-18 | 2017-03-22 | 河南省奶牛生产性能测定中心 | Ecological activity deodorant and preparation method thereof |
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| CN106902631A (en) * | 2017-02-23 | 2017-06-30 | 浙江加百列生物科技有限公司 | A kind of microbial deodorant and production technology |
| CN107058174A (en) * | 2017-03-03 | 2017-08-18 | 神奇国际投资控股有限公司 | A kind of preparation method of efficient deodorizing functional microorganism ferment mould liquid |
| CN107058174B (en) * | 2017-03-03 | 2021-05-25 | 神奇国际投资控股有限公司 | Preparation method of microbial ferment mold liquid with deodorization function |
| CN107189972A (en) * | 2017-07-27 | 2017-09-22 | 蓝德环保科技集团股份有限公司 | Microbial deodorant, preparation method and the usage |
| CN108217971A (en) * | 2017-12-08 | 2018-06-29 | 广州资源环保科技股份有限公司 | A kind of microbial deodorant and preparation method thereof |
| CN108097032A (en) * | 2017-12-29 | 2018-06-01 | 广州广牧丰生物技术有限公司 | A kind of farm's deodorant and the method that poultry production performance is improved with the deodorant |
| CN108271785A (en) * | 2018-01-30 | 2018-07-13 | 南京工业大学 | A kind of biological pesticide and preparation method thereof using the production of bean curd yellow water by fermentation |
| KR101993762B1 (en) * | 2018-08-07 | 2019-07-01 | 주식회사 건영이앤씨 | Liquefied deodorant and fabricating method thereof |
| CN109351175A (en) * | 2018-10-31 | 2019-02-19 | 北京盛源佳业环保科技有限公司 | A kind of quick-acting microbial deodorants |
| CN112295401A (en) * | 2020-12-29 | 2021-02-02 | 南京凯创协同纳米技术有限公司 | Preparation method of spray for removing fishy smell of farmer market |
| CN113980840A (en) * | 2021-10-15 | 2022-01-28 | 辽宁农业职业技术学院 | Microbial deodorant liquid for public environment and preparation method thereof |
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Application publication date: 20150128 |