CN101919488A - Method for producing bioactive feed by utilizing yellow seriflux and waste molasses through fermentation - Google Patents
Method for producing bioactive feed by utilizing yellow seriflux and waste molasses through fermentation Download PDFInfo
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- CN101919488A CN101919488A CN201010289177XA CN201010289177A CN101919488A CN 101919488 A CN101919488 A CN 101919488A CN 201010289177X A CN201010289177X A CN 201010289177XA CN 201010289177 A CN201010289177 A CN 201010289177A CN 101919488 A CN101919488 A CN 101919488A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
A method for producing bioactive feed by utilizing yellow seriflux and waste molasses through fermentation relates to a method for producing bioactive feed. The method solves the problem that the wastes in the current starch factories, such as yellow seriflux and molasses alcohol waste liquor are directly discharged and pollute the environment. The method comprises the following steps: 1. preparing fermentative substrates; 2. activating strains; 3. preparing first-stage seed liquor; 4. preparing second-stage seed liquor; 5. preparing third-stage seed liquor; 6. preparing seed liquor in the workshop; 7. carrying out fermentation production in a 50 m<3>-fermentation tank; and 8. drying, thus obtaining the bioactive feed. The method realizes waste utilization, and no waste liquor is produced in the production process, thus not polluting the environment. The bioactive feed produced by the method contains rich protein, active lactobacillus, trace elements, vitamins and various nutrient substances, the cell number is not less than 2*10<10>/g, the living bacteria rate is not less than 85% and the content of the crude protein is over 50% of the total mass of the feed. The method is applied to the feed field.
Description
Technical field
The present invention relates to a kind of production method of bioactive feed.
Background technology
Can produce a large amount of immersion water in the corn wet production starch process, be commonly called as yellow seriflux.The middle-size and small-size starch factory of current majority with it as trash discharge, soak in the water and contain great number of organic matters, thereby COD (chemical oxygen consumption (COC)) and BOD (BOD) are very high, can cause water oxygen level sharply to descend, and finally cause the large quantities of death of organism in water.In addition, these organic matters also can produce sulfur dioxide and nitrogen oxide in decomposable process, cause atmosphere pollution.
Blackstrap is the accessory substance of glucose production, and its great majority are used for producing alcohol, but produces a large amount of alcohol effluents again simultaneously, and environment is caused severe contamination.Dry matter content is 50%～60% in the blackstrap, and main component is glucose, maltose and compound sugar.Contain several amino acids, water-solubility protein, inorganic salts and vitamin in the yellow seriflux.Therefore, contain multiple nutrients materials such as carbohydrate, protein, amino acid, inorganic elements, vitamin in the mixture of maize pulp-water and blackstrap.These materials can be growth of microorganism prescribing adequate nutrition are provided.
Lactic acid bacteria is a kind of probio, and the important physical function is arranged, and can regulate the body gastrointestinal tract normal flora, keep microecological balance, suppresses corrupt bacteria growing breeding in the enteron aisle, can help digest and calcareous absorption.
Bioactive feed is subjected to more and more raisers' favor as a kind of environment-protecting feed.Have the expert to foretell, bioactive feed will replace current pellet and powder, become main feed.
Summary of the invention
The present invention is in order to solve the direct exhaust emission problem of environment of present starch factory discarded object yellow seriflux and molasses alcohol waste liquid, a kind of method of utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed to be provided.
The method that the present invention utilizes yellow seriflux and waste molasses through fermentation to produce bioactive feed, carry out according to the following steps: one, the preparation of fermentation substrate: blackstrap is mixed with yellow seriflux, make mass concentration and be 15%～25% mixture, add urea according to 2%～5% of mixture quality, regulate pH value to 2.5～4.5 with HCl or the NaOH solution of 5mol/L then, making fermentation substrate behind high temperature sterilization 25～30min under 120 ℃ of conditions; Two, the activation of bacterial classification: picking candida tropicalis and geotrichum candidum bacterial classification, be inoculated into respectively on the brewer's wort solid slant medium, cultivate 36h in 28 ℃; The picking lactic acid bacteria culturers is inoculated on the MRS culture medium, cultivates 16～18h in 37 ℃; Three, the preparation of primary seed solution: candida tropicalis and geotrichum candidum activated spawn that step 2 is made all are inoculated in the triangular flask that the 80ml malt juice liquid medium is housed respectively, cultivate 6～10h under 28～30 ℃ of conditions on shaking table, shaking speed is 150～300r/min; The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80mlMSR fluid nutrient medium, cultivates 24h in 37 ℃; Four, the preparation of secondary seed solution: the fermentation substrate of malt juice liquid medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the candida tropicalis that step 3 is made and the primary seed solution of geotrichum candidum all are inoculated into the seeding tank of 5L respectively by inoculum concentration 4%, cultivate 18～20h under 28～30 ℃ of conditions; The fermentation substrate of MSR fluid nutrient medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions; Five, the preparation of three grades of seed liquor: with the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 200～250L, the secondary seed solution of candida tropicalis that step 4 is made and geotrichum candidum inserts in the 500L seeding tank respectively, inoculum concentration is 20～25L, behind 28～32 ℃ of stirring 8h, continue to cultivate 24h, mended the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates; With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 600～700L, and the secondary seed solution of the lactic acid bacteria that step 4 is made inserts in the 1000L seeding tank, and inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions; Six, prepare seed liquor in the workshop: with the fermentation substrate of the step 1 preparation 5m that packs into
3Seeding tank, liquid amount are 2m
3, three grades of seed liquor of candida tropicalis that step 5 is made and geotrichum candidum are mixed the back and are all inserted 5m
3Seeding tank, in 28～32 ℃ of continuous ventilatings and stirring, Continuous Flow adds the fermentation substrate of step 1 preparation behind the 2h, cultivates 24h; Seven, at 50m
3Carry out fermenting and producing in the fermentation tank: with the fermentation substrate of the step 1 preparation 50m that packs into
3In the fermentation tank, liquid amount is 20m
3, insert the seed liquor 4m that step 6 makes
3, Continuous Flow adds the fermentation substrate of step 1 preparation behind the inoculation 1h, condition under cultivates 36h at 1: 1 in 28～32 ℃, wind liquor ratio, stop to ventilate, regulate pH value to 5.0, insert three grades of seed liquor 500L of the lactic acid bacteria that step 5 makes then, under 33～37 ℃ of conditions, continue fermentation 24h, get zymotic fluid; Eight, drying: the zymotic fluid spray drying tower drying with step 7 makes promptly obtains bioactive feed; The primary seed solution bacterium number average of candida tropicalis that step 3 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml; The primary seed solution bacterium number of the lactic acid bacteria that step 3 makes is 2～3 * 10
8Cfu/ml; The secondary seed solution bacterium number average of candida tropicalis that step 4 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml.
The present invention utilizes yellow seriflux and blackstrap to produce bioactive feed as fermenting raw materials, accomplished twice laid, reduced the direct environmental pollution caused by discharge of yellow seriflux and molasses alcohol waste liquid, reduced the cost of fermentation to produce biological activated feed, do not produce waste liquid in the production process, free from environmental pollution.The bioactive feed that the inventive method is produced contains multiple nutrients materials such as rich in protein, biodiasmin, trace element and vitamin, cell number 〉=2 * 10
10Individual/g, viable bacteria rate 〉=85%, crude protein content account for more than 50% of feed gross mass.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises any combination between each specific embodiment.
The specific embodiment one: the method that present embodiment utilizes yellow seriflux and waste molasses through fermentation to produce bioactive feed, carry out according to the following steps: one, the preparation of fermentation substrate: blackstrap is mixed with yellow seriflux, make mass concentration and be 15%～25% mixture, add urea according to 2%～5% of mixture quality, regulate pH value to 2.5～4.5 with HCl or the NaOH solution of 5mol/L then, making fermentation substrate behind high temperature sterilization 25～30min under 120 ℃ of conditions; Two, the activation of bacterial classification: picking candida tropicalis and geotrichum candidum bacterial classification, be inoculated into respectively on the brewer's wort solid slant medium, cultivate 36h in 28 ℃; The picking lactic acid bacteria culturers is inoculated on the MRS culture medium, cultivates 16～18h in 37 ℃; Three, the preparation of primary seed solution: candida tropicalis and geotrichum candidum activated spawn that step 2 is made all are inoculated in the triangular flask that the 80ml malt juice liquid medium is housed respectively, cultivate 6～10h under 28～30 ℃ of conditions on shaking table, shaking speed is 150～300r/min; The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80mlMSR fluid nutrient medium, cultivates 24h in 37 ℃; Four, the preparation of secondary seed solution: the fermentation substrate of malt juice liquid medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the candida tropicalis that step 3 is made and the primary seed solution of geotrichum candidum all are inoculated into the seeding tank of 5L respectively by inoculum concentration 4%, cultivate 18～20h under 28～30 ℃ of conditions; The fermentation substrate of MSR fluid nutrient medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions; Five, the preparation of three grades of seed liquor: with the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 200～250L, the secondary seed solution of candida tropicalis that step 4 is made and geotrichum candidum inserts in the 500L seeding tank respectively, inoculum concentration is 20～25L, behind 28～32 ℃ of stirring 8h, continue to cultivate 24h, mended the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates; With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 600～700L, and the secondary seed solution of the lactic acid bacteria that step 4 is made inserts in the 1000L seeding tank, and inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions; Six, prepare seed liquor in the workshop: with the fermentation substrate of the step 1 preparation 5m that packs into
3Seeding tank, liquid amount are 2m
3, three grades of seed liquor of candida tropicalis that step 5 is made and geotrichum candidum are mixed the back and are all inserted 5m
3Seeding tank, in 28～32 ℃ of continuous ventilatings and stirring, Continuous Flow adds the fermentation substrate of step 1 preparation behind the 2h, cultivates 24h; Seven, at 50m
3Carry out fermenting and producing in the fermentation tank: with the fermentation substrate of the step 1 preparation 50m that packs into
3In the fermentation tank, liquid amount is 20m
3, insert the seed liquor 4m that step 6 makes
3, Continuous Flow adds the fermentation substrate of step 1 preparation behind the inoculation 1h, condition under cultivates 36h at 1: 1 in 28～32 ℃, wind liquor ratio, stop to ventilate, regulate pH value to 5.0, insert three grades of seed liquor 500L of the lactic acid bacteria that step 5 makes then, under 33～37 ℃ of conditions, continue fermentation 24h, get zymotic fluid; Eight, drying: the zymotic fluid spray drying tower drying with step 7 makes promptly obtains bioactive feed; The primary seed solution bacterium number average of candida tropicalis that step 3 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml; The primary seed solution bacterium number of the lactic acid bacteria that step 3 makes is 2～3 * 10
8Cfu/ml; The secondary seed solution bacterium number average of candida tropicalis that step 4 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml.
Brewer's wort solid slant medium in the present embodiment step 2 soaks powder, 1.5～2g agar and 100ml distilled water by 12g Fructus Hordei Germinatus to be formed, and regulates pH value to 5～6, in 121 ℃ of sterilization 20min.
MSR culture medium in the present embodiment step 2 is by 10g peptone, 10g beef extract, 5g dusty yeast, 2gK
2HPO
4, 2g dibasic ammonium citrate, 5g sodium acetate, 20g glucose, 1ml Tween 80,0.58gMgSO
47H
2O, 0.25gMnSO
44H
2O, 15～20g agar and 1000ml distilled water are formed, and regulate pH value to 6.2～6.4, in 121 ℃ of sterilization 15min.
Malt juice liquid medium in the present embodiment step 3 soaks powder by 20g Fructus Hordei Germinatus and 1000ml distilled water is formed, and regulates pH value to 5～6, in 121 ℃ of sterilization 20min.
MSR fluid nutrient medium in the present embodiment step 3 is by 10g peptone, 10g beef extract, 5g dusty yeast, 2gK
2HPO
4, 2g dibasic ammonium citrate, 5g sodium acetate, 20g glucose, 1ml Tween 80,0.58gMgSO
47H
2O, 0.25gMnSO
44H
2O and 1000ml distilled water are formed, and regulate pH value to 6.2～6.4, in 121 ℃ of sterilization 15min.
Candida tropicalis in the present embodiment step 2 (Candida tropicalis) is preserved in Chinese common micro-organisms culture presevation administrative center, and culture presevation is numbered 2.637; Geotrichum candidum (Geotrichum candidum) is preserved in Chinese common micro-organisms culture presevation administrative center, and culture presevation is numbered 2.1135.
Lactic acid bacteria in the present embodiment step 2 is a pig source Bacillus acidi lactici, buys in Guangzhou industrial microorganism inspection center, and bacterium numbering is GW 1.0659.
The specific embodiment two: what present embodiment and the specific embodiment one were different is: make mass concentration in the step 1 and be 18%～22% mixture.Other is identical with the specific embodiment one.
The specific embodiment three: what present embodiment was different with the specific embodiment one or two is: make mass concentration in the step 1 and be 20% mixture.Other is identical with the specific embodiment one or two.
The specific embodiment four: what present embodiment was different with one of specific embodiment one to three is: add urea according to 3%～4% of mixture quality in the step 1.Other is identical with one of specific embodiment one to three.
The specific embodiment five: what present embodiment was different with one of specific embodiment one to four is: add urea according to 3.5% of mixture quality in the step 1.Other is identical with one of specific embodiment one to four.
The specific embodiment six: what present embodiment was different with one of specific embodiment one to five is: regulate pH value to 3～4 in the step 1.Other is identical with one of specific embodiment one to five.
The specific embodiment seven: what present embodiment was different with one of specific embodiment one to six is: regulate pH value to 3.5 in the step 1.Other is identical with one of specific embodiment one to six.
The specific embodiment eight: what present embodiment was different with one of specific embodiment one to seven is: in the step 1 under 120 ℃ of conditions high temperature sterilization 26～29min.Other is identical with one of specific embodiment one to seven.
The specific embodiment nine: what present embodiment was different with one of specific embodiment one to eight is: in the step 1 under 120 ℃ of conditions high temperature sterilization 27～28min.Other is identical with one of specific embodiment one to eight.
The specific embodiment ten: what present embodiment was different with one of specific embodiment one to nine is: the primary seed solution bacterium number average of candida tropicalis that step 3 makes and geotrichum candidum is 2 * 10
9Cfu/ml.Other is identical with one of specific embodiment one to nine.
The specific embodiment 11: what present embodiment was different with one of specific embodiment one to ten is: the primary seed solution bacterium number of the lactic acid bacteria that step 3 makes is 2.5 * 10
8Cfu/ml.Other is identical with one of specific embodiment one to ten.
The specific embodiment 12: what present embodiment was different with one of specific embodiment one to 11 is: the secondary seed solution bacterium number average of candida tropicalis that step 4 makes and geotrichum candidum is 2 * 10
9Cfu/ml.Other is identical with one of specific embodiment one to 11.
The specific embodiment 13: what present embodiment was different with one of specific embodiment one to 12 is: shaking speed is 200～250r/min in the step 3.Other is identical with one of specific embodiment one to 12.
The specific embodiment 14: the method that present embodiment utilizes yellow seriflux and waste molasses through fermentation to produce bioactive feed, carry out according to the following steps: one, the preparation of fermentation substrate: blackstrap is mixed with yellow seriflux, make mass concentration and be 20% mixture, add urea according to 3.5% of mixture quality, regulate pH value to 3.5 with HCl or the NaOH solution of 5mol/L then, making fermentation substrate behind the high temperature sterilization 30min under 120 ℃ of conditions; Two, the activation of bacterial classification: picking candida tropicalis and geotrichum candidum bacterial classification, be inoculated into respectively on the brewer's wort solid slant medium, cultivate 36h in 28 ℃; The picking lactic acid bacteria culturers is inoculated on the MRS culture medium, cultivates 17h in 37 ℃; Three, the preparation of primary seed solution: candida tropicalis and geotrichum candidum activated spawn that step 2 is made all are inoculated in the triangular flask that the 80ml malt juice liquid medium is housed respectively, cultivate 8h under 30 ℃ of conditions on shaking table, shaking speed is 250r/min; The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80mlMSR fluid nutrient medium, cultivates 24h in 37 ℃; Four, the preparation of secondary seed solution: the fermentation substrate of malt juice liquid medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the candida tropicalis that step 3 is made and the primary seed solution of geotrichum candidum all are inoculated into the seeding tank of 5L respectively by inoculum concentration 4%, cultivate 18h under 30 ℃ of conditions; The fermentation substrate of MSR fluid nutrient medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 12h under 35 ℃ of conditions; Five, the preparation of three grades of seed liquor: with the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 200～250L, the secondary seed solution of candida tropicalis that step 4 is made and geotrichum candidum inserts in the 500L seeding tank respectively, inoculum concentration is 20～25L, behind 28～32 ℃ of stirring 8h, continue to cultivate 24h, mended the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates; With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 600～700L, and the secondary seed solution of the lactic acid bacteria that step 4 is made inserts in the 1000L seeding tank, and inoculum concentration is 50L, cultivates 24h under 35 ℃ of conditions; Six, prepare seed liquor in the workshop: with the fermentation substrate of the step 1 preparation 5m that packs into
3Seeding tank, liquid amount are 2m
3, three grades of seed liquor of candida tropicalis that step 5 is made and geotrichum candidum are mixed the back and are all inserted 5m
3Seeding tank, in 30 ℃ of continuous ventilatings and stirring, Continuous Flow adds the fermentation substrate of step 1 preparation behind the 2h, cultivates 24h; Seven, at 50m
3Carry out fermenting and producing in the fermentation tank: with the fermentation substrate of the step 1 preparation 50m that packs into
3In the fermentation tank, liquid amount is 20m
3, insert the seed liquor 4m that step 6 makes
3, Continuous Flow adds the fermentation substrate of step 1 preparation behind the inoculation 1h, condition under cultivates 36h at 1: 1 in 30 ℃, wind liquor ratio, stop to ventilate, regulate pH value to 5.0, insert three grades of seed liquor 500L of the lactic acid bacteria that step 5 makes then, under 35 ℃ of conditions, continue fermentation 24h, get zymotic fluid; Eight, drying: the zymotic fluid spray drying tower drying with step 7 makes promptly obtains bioactive feed; The primary seed solution bacterium number average of candida tropicalis that step 3 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml; The primary seed solution bacterium number of the lactic acid bacteria that step 3 makes is 2～3 * 10
8Cfu/ml; The secondary seed solution bacterium number average of candida tropicalis that step 4 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml.
Cell number 〉=2 * 10 in the bioactive feed that present embodiment is produced
10Individual/g, viable bacteria rate 〉=85%, crude protein content account for more than 50% of feed gross mass.
Claims (9)
1. utilize yellow seriflux and waste molasses through fermentation to produce the method for bioactive feed, it is characterized in that utilizing the method for yellow seriflux and waste molasses through fermentation production bioactive feed, carry out according to the following steps: one, the preparation of fermentation substrate: blackstrap is mixed with yellow seriflux, make mass concentration and be 15%～25% mixture, add urea according to 2%～5% of mixture quality, regulate pH value to 2.5～4.5 with HCl or the NaOH solution of 5mol/L then, making fermentation substrate behind high temperature sterilization 25～30min under 120 ℃ of conditions; Two, the activation of bacterial classification: picking candida tropicalis and geotrichum candidum bacterial classification, be inoculated into respectively on the brewer's wort solid slant medium, cultivate 36h in 28 ℃; The picking lactic acid bacteria culturers is inoculated on the MRS culture medium, cultivates 16～18h in 37 ℃; Three, the preparation of primary seed solution: candida tropicalis and geotrichum candidum activated spawn that step 2 is made all are inoculated in the triangular flask that the 80ml malt juice liquid medium is housed respectively, cultivate 6～10h under 28～30 ℃ of conditions on shaking table, shaking speed is 150～300r/min; The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80mlMSR fluid nutrient medium, cultivates 24h in 37 ℃; Four, the preparation of secondary seed solution: the fermentation substrate of malt juice liquid medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the candida tropicalis that step 3 is made and the primary seed solution of geotrichum candidum all are inoculated into the seeding tank of 5L respectively by inoculum concentration 4%, cultivate 18～20h under 28～30 ℃ of conditions; The fermentation substrate of MSR fluid nutrient medium with the step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of a seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions; Five, the preparation of three grades of seed liquor: with the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 200～250L, the secondary seed solution of candida tropicalis that step 4 is made and geotrichum candidum inserts in the 500L seeding tank respectively, inoculum concentration is 20～25L, behind 28～32 ℃ of stirring 8h, continue to cultivate 24h, mended the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates; With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 600～700L, and the secondary seed solution of the lactic acid bacteria that step 4 is made inserts in the 1000L seeding tank, and inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions; Six, prepare seed liquor in the workshop: with the fermentation substrate of the step 1 preparation 5m that packs into
3Seeding tank, liquid amount are 2m
3, three grades of seed liquor of candida tropicalis that step 5 is made and geotrichum candidum are mixed the back and are all inserted 5m
3Seeding tank, in 28～32 ℃ of continuous ventilatings and stirring, Continuous Flow adds the fermentation substrate of step 1 preparation behind the 2h, cultivates 24h; Seven, at 50m
3Carry out fermenting and producing in the fermentation tank: with the fermentation substrate of the step 1 preparation 50m that packs into
3In the fermentation tank, liquid amount is 20m
3, insert the seed liquor 4m that step 6 makes
3, Continuous Flow adds the fermentation substrate of step 1 preparation behind the inoculation 1h, condition under cultivates 36h at 1: 1 in 28～32 ℃, wind liquor ratio, stop to ventilate, regulate pH value to 5.0, insert three grades of seed liquor 500L of the lactic acid bacteria that step 5 makes then, under 33～37 ℃ of conditions, continue fermentation 24h, get zymotic fluid; Eight, drying: the zymotic fluid spray drying tower drying with step 7 makes promptly obtains bioactive feed; The primary seed solution bacterium number average of candida tropicalis that step 3 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml; The primary seed solution bacterium number of the lactic acid bacteria that step 3 makes is 2～3 * 10
8Cfu/ml; The secondary seed solution bacterium number average of candida tropicalis that step 4 makes and geotrichum candidum is 1～3 * 10
9Cfu/ml.
2. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 1 is characterized in that making in the step 1 mass concentration and is 18%～22% mixture.
3. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 1 is characterized in that making in the step 1 mass concentration and is 20% mixture.
4. according to claim 1, the 2 or 3 described methods of utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed, it is characterized in that adding urea according to 3%～4% of mixture quality in the step 1.
5. according to claim 1, the 2 or 3 described methods of utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed, it is characterized in that adding urea according to 3.5% of mixture quality in the step 1.
6. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 4 is characterized in that regulating in the step 1 pH value to 3～4.
7. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 4 is characterized in that regulating in the step 1 pH value to 3.5.
8. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 6 is characterized in that in the step 1 high temperature sterilization 26～29min under 120 ℃ of conditions.
9. the method for utilizing yellow seriflux and waste molasses through fermentation to produce bioactive feed according to claim 6 is characterized in that in the step 1 high temperature sterilization 27～28min under 120 ℃ of conditions.
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| CN102113621A (en) * | 2011-03-15 | 2011-07-06 | 黑龙江省轻工科学研究院 | Method for producing straw feed by utilizing alcohol waste liquid |
| CN103098979A (en) * | 2013-01-16 | 2013-05-15 | 上海清美绿色食品有限公司 | Method for producing single-cell protein feed by utilizing bean product waste water |
| CN103621316A (en) * | 2013-12-20 | 2014-03-12 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN104307014A (en) * | 2014-09-26 | 2015-01-28 | 任杰 | Microbial deodorant and preparation method thereof |
| CN113615766A (en) * | 2021-08-13 | 2021-11-09 | 点斗基因科技(南京)有限公司 | Preparation and application of composite plant extract and probiotic biological fermentation feed |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101161094A (en) * | 2007-10-22 | 2008-04-16 | 桂林工学院 | Method for producing single cell protein feed using waste molasses |
| CN101273749A (en) * | 2008-04-15 | 2008-10-01 | 四川宜宾全福饲料有限公司 | Method of mixed fermentation treatment using vinasse as main feed raw material |
| CN101709272A (en) * | 2009-11-26 | 2010-05-19 | 秦皇岛骊骅淀粉股份有限公司 | Method for preparing feed yeast |
| CN101731450A (en) * | 2009-12-21 | 2010-06-16 | 江苏联海生物科技有限公司 | Preparation method of single cell protein feed by taking acetone butanol fermentation wastewater as raw materials |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101161094A (en) * | 2007-10-22 | 2008-04-16 | 桂林工学院 | Method for producing single cell protein feed using waste molasses |
| CN101273749A (en) * | 2008-04-15 | 2008-10-01 | 四川宜宾全福饲料有限公司 | Method of mixed fermentation treatment using vinasse as main feed raw material |
| CN101709272A (en) * | 2009-11-26 | 2010-05-19 | 秦皇岛骊骅淀粉股份有限公司 | Method for preparing feed yeast |
| CN101731450A (en) * | 2009-12-21 | 2010-06-16 | 江苏联海生物科技有限公司 | Preparation method of single cell protein feed by taking acetone butanol fermentation wastewater as raw materials |
Non-Patent Citations (1)
| Title |
|---|
| 《饲料研究》 20071231 曾鸿鹄等 糖蜜废液生产单细胞蛋白的研究 , 第9期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102113621A (en) * | 2011-03-15 | 2011-07-06 | 黑龙江省轻工科学研究院 | Method for producing straw feed by utilizing alcohol waste liquid |
| CN103098979A (en) * | 2013-01-16 | 2013-05-15 | 上海清美绿色食品有限公司 | Method for producing single-cell protein feed by utilizing bean product waste water |
| CN103621316A (en) * | 2013-12-20 | 2014-03-12 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN103621316B (en) * | 2013-12-20 | 2015-01-21 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN104307014A (en) * | 2014-09-26 | 2015-01-28 | 任杰 | Microbial deodorant and preparation method thereof |
| CN113615766A (en) * | 2021-08-13 | 2021-11-09 | 点斗基因科技(南京)有限公司 | Preparation and application of composite plant extract and probiotic biological fermentation feed |
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