CN103098979A - Method for producing single-cell protein feed by utilizing bean product waste water - Google Patents
Method for producing single-cell protein feed by utilizing bean product waste water Download PDFInfo
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Abstract
The invention provides a method for producing a single-cell protein feed by utilizing bean product waste water. A modern microorganism fermentation technology is adopted, and through the steps of high-speed strain liquid culture, deep layer liquid culture of the bean product waste water and the like, a great amount of yellow serofluid, bean soaking water, soybean whey waste water and the like generated in bean product production by utilizing a plurality of target microorganisms of mycete, fungi and bacteria are subjected to a deep layer liquid fermentation technology to produce a protein feed rich in single-cell protein and various biological metabolism enzymes, so that the requirement of increasing protein feeds can be met, and the method for producing the single-cell protein feed by utilizing bean product waste water is a novel technology which turns waste into wealth, and reduces the environment pollution caused by food processing waste water greatly.
Description
Technical field
The present invention relates to a kind of technique of utilizing soybean wastewater manufacture order cell protein feed, multiple probio, biology enzyme and bioactivator that feed after fermentation contains simultaneously and is of value to the livestock and poultry abilities of digestive and absorption, improves immunity and cold-resistant copingability belong to the agricultural products technical field.
Background technology
Bean product are the food that processes from strand as primary raw material take beans such as soybean, mung bean, pea, broad beans.Everybody the most familiar every day edible soya-bean milk, soymilk, bean curd be all representative wherein.Generally all to soak by beans in bean product processing, grind, separation, washery slag, the link such as solidify, to consume or use a large amount of water in each link, an archaism " bean curd is that water is done " is therefore arranged, " seven minutes water of three minutes soybean ", China is the upper maximum bean product production and consumption state of practice, every day, a large amount of bean product produced a large amount of soybean wastewaters in producing, and the characteristics of soybean wastewater are that the large organic concentration of the discharge capacity of waste water is high, complicated component.Take bean curd production as example, yellow swill (claiming again yellow seriflux) COD is up to 20000 to 30000mg/L, and soaking bean water COD is 4000 to 8000mg/L, and various waste water organic principles are very complicated.Soaking bean water mainly contain the oligosaccharides such as water-soluble nonprotein nitrogen, tax threose, raffinose, the organic acid such as citric acid and water soluble vitamin, mineral matter etc. in addition, also have the colors such as isoflavones.The composition of yellow swill is more complicated, except all the components that contains soaking bean water, also contains protein (soy whey, soybean haemoglutinin, trypsin ihhibitor etc.), amino acid, lipid etc.Bean curd is produced in water for cleaning and is contained soy whey, carbohydrate, bean dregs and cleaning agent etc.
After these soybean wastewaters were collected, the sewage treatment plant that generally enters food factory processed, and after processing, environment has obtained improvement, but in waste water, a large amount of water-solubility protein, grease, total reducing sugar, water soluble vitamins also has been wasted.in order to reclaim the fertile absorber resource in soybean wastewater, yet many employing membrane separation techniques are separated compound sugar and the protein in waste water at present., the high operating cost that the replacing of film and cleaning bring makes its economic benefit very little, seriously having restricted it applies, contain a large amount of protein in soybean wastewater, polysaccharide, the living beings such as grease, the very good material of manufacture order cell protein (SCP). the high concentration whey wastewater that utilizes the soybean protein production technology to discharge carries out SCP production, when SCP produces good economic benefits, can significantly cut down the COD mass concentration of soybean wastewater, reduce the disposal cost of follow-up waste water and the discharge capacity of methane and CO2.
Single cell protein (SCP) also be microprotein, and it is with many industrial and agricultural wastes and the artificial microbial cells of cultivating of oil waste material.Thereby single cell protein is not a kind of true protein, but is reached the cytoplasm group of the compositions of mixtures such as nitrogen-containing compound, vitamin and inorganic compound that are not protein by protein, fat, carbohydrate, nucleic acid.The contained nutriment of single cell protein is very abundant.Wherein, protein content is up to 40%～80%, and is higher by 10%～20% than soybean, higher more than 20% than meat, fish, cheese; Amino acid whose composition is comparatively complete, contains 8 seed amino acids of needed by human.Also contain multivitamin, carbohydrate, lipid, mineral matter in single cell protein, and abundant enzyme and bioactivator, as coacetylase, ubiquinone, glutathione, ergosterol etc.Single cell protein both can improve the edible needs that satisfy human amino acid's protein of people, a kind of outstanding forage protein raw material especially, cell protein feed [1] (Single Cell Protein) mainly refer to saccharomycete, bacterium, mould and the alga cells organism etc. that produce by fermentation process.Single-cell protein feed is nutritious, protein content is higher, and contains 18～20 seed amino acids, and component is complete, is rich in multivitamin.Because its nutrition-allocated proportion is complete, can directly use as complete feed.In addition, the production of single-cell protein feed has the advantages such as the speed of breeding is fast, production efficiency is high, floor space is little, weatherproof, therefore, in the situation that world today's protein resource wretched insufficiency, the production of development single-cell protein feed more and more is subject to the attention of various countries.
Microbe species for the production of single cell protein is a lot, comprise bacterium, actinomyces, saccharomycete, mould and some protist, but under different condition, growth of microorganism is subject to the impact of the many factors such as medium component, acid-base value, temperature, simultaneously, different microorganisms can produce antagonism mutually because metabolic pathway is different, therefore, utilize microbial technique manufacture order cell protein feed should primarily solve the assembly of fermented bacterium.Production bacterial classification of the present invention is formed by following inoculating proportion: 1) geotrichum candidum (Geotrichum candidum link), the protein hydrolysate ability is strong, the good mycoprotein of adaptive capacity to environment is of high nutritive value, can be edible and feed use, geotrichum candidum can also synthctic fat, can utilize the organic wastewater of sugar refinery, brewery and other food factories to produce forage protein 2); 2) candida utili (Candida utilis), can utilize paper pulp, honey, potato starch and wood hydrolysis liquid, except can effectively utilizing various monose and compound sugar, macromolecular complex that can also the decomposition of cellulose class, be produce in the world at present .SCP main bacteria seed it--; 3) photosynthetic bacteria (PhotoSyntheticBacteria is called for short PSB, that a kind of luminous energy that can utilize is as the bacterium of energy source, the photosynthetic bacteria strong adaptability, can restrain oneself the organic wastewater of high concentration, the poisonous substances such as phenol, cyanogen are had necessarily stand and capacity of decomposition, has stronger decomposition and inversion ability, photosynthetic bacteria is a kind of nutritious, bacterium of being of high nutritive value, thalline contains abundant amino acid, folic acid, b family vitamin, especially vitamin b12 and biotin content are higher, also have the physiological activator ubiquinone.The volume of photosynthetic bacteria is 1/20th of chlorella, is also a kind of outstanding cell protein feed.
the protein raw materials deficiency is a most thorny problem of current feedstuff industry, the whole world is faced with the reality of protein resource scarcity at present, domestic at present, the primary raw material dregs of beans of foreign data albumen, holding at high price of fish meal, seek and develop new protein resource and become the feed industry urgent problem, taking full advantage of existing resource turns waste into wealth, develop brand-new protein feed, the nonhazardous protection of the environment, become the focus of research in the future, how by means of microbial technique, utilize discarded protein raw materials, exploitation high protein full price novel fodder, vegetable protein and inorganic nitrogen are converted into the microprotein of animal capable good absorption.It is an important topic of feed industry research and development from now on.
Summary of the invention
the protein raw materials deficiency is a most thorny problem of current feedstuff industry, the whole world is faced with the reality of protein resource scarcity at present, domestic at present, the primary raw material dregs of beans of foreign data albumen, holding at high price of fish meal, seek and develop new protein resource and become the feed industry urgent problem, taking full advantage of existing resource turns waste into wealth, develop brand-new protein feed, the nonhazardous protection of the environment, become the focus of research in the future, how by means of microbial technique, utilize discarded protein raw materials, exploitation high protein full price novel fodder, vegetable protein and inorganic nitrogen are converted into the microprotein of animal capable good absorption.It is an important topic of feed industry research and development from now on.
The objective of the invention is to adopt modern microbial fermentation technology, utilize a large amount of yellow swill (yellow seriflux) that mould, fungi, a plurality of objective microbes of bacterium produce during bean product are produced, soaking bean water, soya whey wastewater etc., adopt deep fermentation technique, produce a kind of protein feed that is rich in single cell protein and various biotransferases, to satisfy ever-increasing protein feed needs, be a kind ofly to turn waste into wealth, significantly reduce a kind of environmental improvement new technology of food processing wastewater environmental pollution.
Technical problem to be solved by this invention is, overcomes problems of the prior art, and a kind of method of utilizing soybean wastewater manufacture order cell protein feed is provided.
In order to address the above problem, technical scheme of the present invention is such:
A kind of method of utilizing soybean wastewater manufacture order cell protein feed comprises the following steps:
The first step, the production production of hybrid seeds
Will be through the geotrichum candidum that produces the bacterium experiment sieving, produce protein silk Candida, photosynthetic bacteria and transfer respectively in the eggplant bottle that PDA agar medium, malt extract medium, photosynthetic bacteria culture medium are housed, cultivated 45-50 hour at 30-35 ℃ of temperature, treat that eggplant bottle surface lawn is covered with, and can take out when in white, band is yellow, then put into the refrigerator of 2-6 ℃ and preserve;
Second step, bacterial classification liquid at high speed are cultivated
(1), the bacterial classification of step 1 being cultivated takes by following percentage by weight: produce protein silk Candida 30-36%, photosynthetic bacteria 20-27%, geotrichum candidum 32-50%;
(2) the compound cultivation of bacterial classification: the bacterial classification that takes more than inciting somebody to action is put into the culture medium of following proportioning in the 1:6-10 ratio: molasses 15%, peptone 3%, corn flour 4%, sodium chloride 0.5%, yeast extract 0.5%, magnesium phosphate 0.05%, potassium dihydrogen sulfate 0.05%, sterilized water 77.4%.Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 26-28 ℃, and throughput 1:0.6, fermentation time are 18-20 hour, fermentation ends when the ph value reaches 4.5-6;
The 3rd step soybean wastewater deep-layer liquid is cultivated
Culture medium preparation and sterilization: culture medium forms and percentage: yellow seriflux 50-55%, wash beans water 27-35%, molasses 12-15%, starch 3-5%, in the fluid nutrient medium of bacterium; Add in airtight fermentation tank 120-128 ℃, 30-45min high-temperature sterilization in above ratio obtaining liq fermentation medium;
The bacterial classification that takes in above second step is put into fluid nutrient medium by weight 3-5% cultivate, the ph value transfers to 6.9-7.4; Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 28-30 ℃, and throughput 1:0.6, fermentation time are 24-30 hour, and the ph value drops to 3.5-4.0 by the time, occurs a large amount of yeast cells in the liquid microscopy, cultivates and finishes;
The 4th step filtered and extracts and concentrate
With the standing 18-20 of zymotic fluid hour that adds auxiliary material, filter by disk centrifugal separator, collect filtered fluid, filter by ionic membrane, obtain the filtration residue of single cell protein, dry below moisture to 20% for 40-45 ℃ in baking oven, obtain single cell protein product of the present invention, store after packing.
Beneficial effect, the present invention adopts modern microbial fermentation technology, utilize a large amount of yellow swill (yellow seriflux) that mould, fungi, a plurality of objective microbes of bacterium produce during bean product are produced, soaking bean water, soya whey wastewater etc., adopt deep fermentation technique, produce a kind of protein feed that is rich in single cell protein and various biotransferases, to satisfy ever-increasing protein feed needs, be a kind ofly to turn waste into wealth, significantly reduce a kind of new agricultural technology of food processing wastewater environmental pollution.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
The first step, the production production of hybrid seeds will be through the geotrichum candidums that produce the bacterium experiment sieving, produce protein silk Candida, photosynthetic bacteria transfers respectively in the eggplant bottle that PDA agar medium, malt extract medium, photosynthetic bacteria culture medium are housed, cultivated 45-50 hour at 30-35 ℃ of temperature, treat that eggplant bottle surface lawn is covered with, and can take out when in white, band is yellow, then put into the refrigerator of 2-6 ℃ and preserve;
Second step, bacterial classification liquid at high speed are cultivated
(1), the bacterial classification of step 1 being cultivated takes by following percentage by weight: produce protein silk Candida 35%, photosynthetic bacteria 25%, geotrichum candidum 40%;
(2) the compound cultivation of bacterial classification: the bacterial classification that takes more than inciting somebody to action is put into the culture medium of following proportioning in the 1:6 ratio: molasses 15%, peptone 3%, corn flour 4%, sodium chloride 0.5%, yeast extract 0.5%, magnesium phosphate 0.05%, potassium dihydrogen sulfate 0.05%, sterilized water 77.4%.Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 26-28 ℃, and throughput 1:0.6, fermentation time are 18-20 hour, fermentation ends when the ph value reaches 4.5-6;
The 3rd step soybean wastewater deep-layer liquid is cultivated
Culture medium preparation and sterilization: culture medium forms and percentage: yellow seriflux 52%, wash beans water 32%, molasses 13%, starch 3%, in the fluid nutrient medium of bacterium; Add in airtight fermentation tank 120-128 ℃, 35min high-temperature sterilization in above ratio obtaining liq fermentation medium;
The bacterial classification that takes in above second step is put into fluid nutrient medium by weight 3-5% cultivate, the ph value transfers to 6.9-7.4; Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 28-30 ℃, and throughput 1:0.6, fermentation time are 24 hours, and the ph value drops to 3.5-4.0 by the time, occurs a large amount of yeast cells in the liquid microscopy, cultivates and finishes;
The 4th step filtered and extracts and concentrate
With the standing 18-20 of zymotic fluid hour that adds auxiliary material, filter by disk centrifugal separator, collect filtered fluid, filter by ionic membrane, obtain the filtration residue of single cell protein, dry below moisture to 20% for 40-45 ℃ in baking oven, obtain single cell protein product of the present invention, store after packing.
Embodiment 2
The first step, the production production of hybrid seeds are with embodiment 1
Second step, bacterial classification liquid at high speed are cultivated
(1), the bacterial classification of step 1 being cultivated takes by following percentage by weight: produce protein silk Candida 34%, photosynthetic bacteria 22%, geotrichum candidum 34%;
(2) the compound cultivation of bacterial classification: the bacterial classification that takes more than inciting somebody to action is put into the culture medium of following proportioning in the 1:10 ratio: molasses 15%, peptone 3%, corn flour 4%, sodium chloride 0.5%, yeast extract 0.5%, magnesium phosphate 0.05%, potassium dihydrogen sulfate 0.05%, sterilized water 77.4%.Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 28 ℃, and throughput 1:0.6, fermentation time are 18-20 hour, fermentation ends when the ph value reaches 4.5-6;
The 3rd step soybean wastewater deep-layer liquid is cultivated
Culture medium preparation and sterilization: culture medium forms and percentage: yellow seriflux 50-55%, wash beans water 27-35%, molasses 12-15%, starch 3-5%, in the fluid nutrient medium of bacterium; Add in airtight fermentation tank 128 ℃, 45min high-temperature sterilization in above ratio obtaining liq fermentation medium;
The bacterial classification that takes in above second step is put into fluid nutrient medium by weight 3-5% cultivate, the ph value transfers to 7.0; Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 28-30 ℃, and throughput 1:0.6, fermentation time are 24-30 hour, and the ph value drops to 3.5-4.0 by the time, occurs a large amount of yeast cells in the liquid microscopy, cultivates and finishes;
The 4th step filtered and extracts and concentrate
With the standing 18-20 of zymotic fluid hour that adds auxiliary material, filter by disk centrifugal separator, collect filtered fluid, filter by ionic membrane, obtain the filtration residue of single cell protein, dry below moisture to 20% for 40-45 ℃ in baking oven, obtain single cell protein product of the present invention, store after packing.
Above demonstration and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not subjected to the restriction of above-mentioned example; that describes in above-mentioned example and specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. a method of utilizing soybean wastewater manufacture order cell protein feed, is characterized in that, comprises the following steps:
The first step, the production production of hybrid seeds
Will be through the geotrichum candidum that produces the bacterium experiment sieving, produce protein silk Candida, photosynthetic bacteria and transfer respectively in the eggplant bottle that PDA agar medium, malt extract medium, photosynthetic bacteria culture medium are housed, cultivated 45-50 hour at 30-35 ℃ of temperature, treat that eggplant bottle surface lawn is covered with, and can take out when in white, band is yellow, then put into the refrigerator of 2-6 ℃ and preserve;
Second step, bacterial classification liquid at high speed are cultivated
(1), the bacterial classification of step 1 being cultivated takes by following percentage by weight: produce protein silk Candida 30-36%, photosynthetic bacteria 20-27%, geotrichum candidum 32-50%;
(2) the compound cultivation of bacterial classification: the bacterial classification that takes more than inciting somebody to action is put into the culture medium of following proportioning in the 1:6-10 ratio: molasses 15%, peptone 3%, corn flour 4%, sodium chloride 0.5%, yeast extract 0.5%, magnesium phosphate 0.05%, potassium dihydrogen sulfate 0.05%, sterilized water 77.4%.Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 26-28 ℃, and throughput 1:0.6, fermentation time are 18-20 hour, fermentation ends when the ph value reaches 4.5-6;
The 3rd step soybean wastewater deep-layer liquid is cultivated
The bacterial classification that takes in above second step is put into fluid nutrient medium by weight 3-5% cultivate, the ph value transfers to 6.9-7.4; Put into fermentation tank and cultivate, speed of agitator is 200r/min-240r/min, and cultivation temperature is 28-30 ℃, and throughput 1:0.6, fermentation time are 24-30 hour, and the ph value drops to 3.5-4.0 by the time, occurs a large amount of yeast cells in the liquid microscopy, cultivates and finishes;
The 4th step filtered and extracts and concentrate
With the standing 18-20 of zymotic fluid hour that adds auxiliary material, filter by disk centrifugal separator, collect filtered fluid, filter by ionic membrane, obtain the filtration residue of single cell protein, the 40-45 degree is dried below moisture to 20% in baking oven, obtain single cell protein product of the present invention, store after packing.
2. a kind of method of utilizing soybean wastewater manufacture order cell protein feed according to claim 1, it is characterized in that, in described the 3rd step, culture medium forms and percentage: yellow seriflux 50-55%, wash beans water 27-35%, molasses 12-15%, starch 3-5%, in the fluid nutrient medium of bacterium; Add in airtight fermentation tank 120-128 ℃, 30-45min high-temperature sterilization in above ratio obtaining liq fermentation medium.
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| CN103621316A (en) * | 2013-12-20 | 2014-03-12 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN103642707A (en) * | 2013-12-03 | 2014-03-19 | 湖北工业大学 | Method for producing candida for feeds by fermenting high-salt-content amino acid wastewater |
| CN103907758A (en) * | 2014-04-23 | 2014-07-09 | 安徽五粮泰生物工程股份有限公司 | Preparation method of feeding fermentative bean sprout feed |
| CN104212743A (en) * | 2014-08-22 | 2014-12-17 | 华南理工大学 | Culture medium for high-density cultivation of lactobacillus amylolyticus L6 and cultivation method of culture medium |
| CN106191008A (en) * | 2016-07-08 | 2016-12-07 | 自然生物远景有限公司 | Utilize food and drink to produce waste water and produce method and the product of the microbial compound enzyme preparation for albumen feedstuff enzymolysis |
| CN106478163A (en) * | 2016-10-11 | 2017-03-08 | 临邑禹王植物蛋白有限公司 | A kind of soy protein wastewater sludge organism fertilizer and preparation method thereof |
| CN108456020A (en) * | 2018-02-27 | 2018-08-28 | 东北农业大学 | The method for preparing organic fertilizer raw material using bean product processing waste |
| CN108497187A (en) * | 2018-03-28 | 2018-09-07 | 周口广安农牧科技有限公司 | A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans |
| CN109170144A (en) * | 2018-11-05 | 2019-01-11 | 扬州市职业大学 | Method based on multi-cultur es cooperative fermentation preparation yellow serofluid and Cottonseed Meal mixed type biology feed |
| CN110881583A (en) * | 2018-09-10 | 2020-03-17 | 宿迁益佰饲料有限公司 | Green and environment-friendly river crab feed prepared from soybeans |
| CN110982706A (en) * | 2019-12-23 | 2020-04-10 | 中国科学院成都生物研究所 | Geotrichum candidum and method for producing single-cell protein by treating high-ammonia nitrogen biogas slurry with same |
| CN111034852A (en) * | 2020-01-10 | 2020-04-21 | 安徽省农业科学院畜牧兽医研究所 | Fermented yellow serofluid for lactating dairy cows and preparation method thereof |
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| CN103642707A (en) * | 2013-12-03 | 2014-03-19 | 湖北工业大学 | Method for producing candida for feeds by fermenting high-salt-content amino acid wastewater |
| CN103621316A (en) * | 2013-12-20 | 2014-03-12 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN103621316B (en) * | 2013-12-20 | 2015-01-21 | 河北大学 | Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material |
| CN103907758A (en) * | 2014-04-23 | 2014-07-09 | 安徽五粮泰生物工程股份有限公司 | Preparation method of feeding fermentative bean sprout feed |
| CN103907758B (en) * | 2014-04-23 | 2016-02-17 | 安徽五粮泰生物工程股份有限公司 | The preparation method of a kind of feeding fermented type bean sprouts feed |
| CN104212743A (en) * | 2014-08-22 | 2014-12-17 | 华南理工大学 | Culture medium for high-density cultivation of lactobacillus amylolyticus L6 and cultivation method of culture medium |
| CN106191008A (en) * | 2016-07-08 | 2016-12-07 | 自然生物远景有限公司 | Utilize food and drink to produce waste water and produce method and the product of the microbial compound enzyme preparation for albumen feedstuff enzymolysis |
| CN106478163A (en) * | 2016-10-11 | 2017-03-08 | 临邑禹王植物蛋白有限公司 | A kind of soy protein wastewater sludge organism fertilizer and preparation method thereof |
| CN108456020A (en) * | 2018-02-27 | 2018-08-28 | 东北农业大学 | The method for preparing organic fertilizer raw material using bean product processing waste |
| CN108497187A (en) * | 2018-03-28 | 2018-09-07 | 周口广安农牧科技有限公司 | A kind of yellow serofluid promotees the preparation method of newborn glycolysis dregs of beans |
| CN110881583A (en) * | 2018-09-10 | 2020-03-17 | 宿迁益佰饲料有限公司 | Green and environment-friendly river crab feed prepared from soybeans |
| CN109170144A (en) * | 2018-11-05 | 2019-01-11 | 扬州市职业大学 | Method based on multi-cultur es cooperative fermentation preparation yellow serofluid and Cottonseed Meal mixed type biology feed |
| CN110982706A (en) * | 2019-12-23 | 2020-04-10 | 中国科学院成都生物研究所 | Geotrichum candidum and method for producing single-cell protein by treating high-ammonia nitrogen biogas slurry with same |
| CN111034852A (en) * | 2020-01-10 | 2020-04-21 | 安徽省农业科学院畜牧兽医研究所 | Fermented yellow serofluid for lactating dairy cows and preparation method thereof |
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Application publication date: 20130515 |