CN106578723A - Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia - Google Patents
Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia Download PDFInfo
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- CN106578723A CN106578723A CN201611171531.2A CN201611171531A CN106578723A CN 106578723 A CN106578723 A CN 106578723A CN 201611171531 A CN201611171531 A CN 201611171531A CN 106578723 A CN106578723 A CN 106578723A
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- fermented feed
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- lentinus
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- 241000276707 Tilapia Species 0.000 title claims abstract description 27
- 235000013372 meat Nutrition 0.000 title claims abstract description 16
- 238000009395 breeding Methods 0.000 title claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 229920002472 Starch Polymers 0.000 claims abstract description 14
- 229930003231 vitamin Natural products 0.000 claims abstract description 14
- 235000013343 vitamin Nutrition 0.000 claims abstract description 14
- 239000011782 vitamin Substances 0.000 claims abstract description 14
- 229940088594 vitamin Drugs 0.000 claims abstract description 14
- 239000008107 starch Substances 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 240000000599 Lentinula edodes Species 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 30
- 239000000203 mixture Substances 0.000 claims description 21
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 235000001715 Lentinula edodes Nutrition 0.000 claims description 14
- -1 compound vitamin Chemical class 0.000 claims description 14
- 240000007594 Oryza sativa Species 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 12
- 235000009566 rice Nutrition 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 10
- 241000251468 Actinopterygii Species 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 229930003270 Vitamin B Natural products 0.000 claims description 8
- 229930003268 Vitamin C Natural products 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 235000019156 vitamin B Nutrition 0.000 claims description 8
- 239000011720 vitamin B Substances 0.000 claims description 8
- 235000019154 vitamin C Nutrition 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 235000019890 Amylum Nutrition 0.000 claims description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- 230000001488 breeding effect Effects 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229920001592 potato starch Polymers 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 4
- 241000186361 Actinobacteria <class> Species 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 239000002068 microbial inoculum Substances 0.000 claims description 4
- 240000003183 Manihot esculenta Species 0.000 claims description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 2
- 101710180313 Protease 3 Proteins 0.000 claims description 2
- 229930003316 Vitamin D Natural products 0.000 claims description 2
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 2
- 229930003427 Vitamin E Natural products 0.000 claims description 2
- 239000013039 cover film Substances 0.000 claims description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 2
- 235000019166 vitamin D Nutrition 0.000 claims description 2
- 239000011710 vitamin D Substances 0.000 claims description 2
- 150000003710 vitamin D derivatives Chemical class 0.000 claims description 2
- 235000019165 vitamin E Nutrition 0.000 claims description 2
- 229940046009 vitamin E Drugs 0.000 claims description 2
- 239000011709 vitamin E Substances 0.000 claims description 2
- 229940046008 vitamin d Drugs 0.000 claims description 2
- 210000000582 semen Anatomy 0.000 claims 2
- 241001037822 Bacillus bacterium Species 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 239000013049 sediment Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 abstract description 8
- 235000012054 meals Nutrition 0.000 abstract description 6
- 239000000835 fiber Substances 0.000 abstract description 5
- 230000029087 digestion Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 108010064851 Plant Proteins Proteins 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000009360 aquaculture Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 238000013329 compounding Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 150000003722 vitamin derivatives Chemical class 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 6
- 108010059892 Cellulase Proteins 0.000 description 5
- 241000233866 Fungi Species 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 5
- 229940106157 cellulase Drugs 0.000 description 5
- 235000012343 cottonseed oil Nutrition 0.000 description 5
- 239000001301 oxygen Substances 0.000 description 5
- 229910052760 oxygen Inorganic materials 0.000 description 5
- 241000186016 Bifidobacterium bifidum Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 210000004243 sweat Anatomy 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- 230000001332 colony forming effect Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000276618 Perciformes Species 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 101100279436 Caenorhabditis elegans egg-2 gene Proteins 0.000 description 1
- 241001441694 Callichthyidae Species 0.000 description 1
- 241000276616 Cichlidae Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 235000014897 Streptococcus lactis Nutrition 0.000 description 1
- 241000810789 Streptomyces albospinus Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- FYHXNYLLNIKZMR-UHFFFAOYSA-N calcium;carbonic acid Chemical compound [Ca].OC(O)=O FYHXNYLLNIKZMR-UHFFFAOYSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
Belonging to the technical field of aquaculture, the invention relates to a letinous edodes residue fermented feed and a breeding method for raising the meat quality of genetically improved farmed tilapia. The feed is obtained by subjecting letinous edodes residue, meal starch and plant starch to bacterial liquid fermentation treatment, and then conducting compounding with composite vitamin. According to the invention, through fermentation treatment on letinous edodes residue, the protein, plant fiber and the like contained therein can be effectively decomposed, and can be absorbed by genetically improved farmed tilapia, nutritional ingredients therein can be suitable for digestion and absorption of genetically improved farmed tilapia, so that the meat can be more tender and has little crude fiber, the taste of genetically improved farmed tilapia is improved, also the fact content of meat is reduced, thus being more conducive to eating health.
Description
Technical field
The present invention relates to a kind of Lentinus Edodes fungus residue fermented feed and improve the method for breeding of gift tilapia meat, belong to Aquatic product
Cultural technique field.
Background technology
Tilapia belongs to Perciformess（Perciformes）Callichthyidae（Cichlidae）Tilapia belongs to（tilapia）Fish, about
There are nearly hundred kinds, be distributed mainly on Africa.Gift tilapia robs food power by force, and growth is fast, 25-30% faster than bolti, than Buddhist nun difficult to understand
Fish fast more than 20%, then Seedling can grow to more than 800g then, make it become the first-selection of Chinese Rofe fish culture in nearly 10 years.
But, the meat relatively thick, mouthfeel of gift tilapia is not so good as other tilapia kinds.
Edible fungi is because which is nutritious, delicious flavour and extremely common people's favor.China is edible fungi understanding, utilizes, cultivates
Earliest country, after reform and opening-up, edible fungi of china industry development achieves prominent achievement.The bacteria residue that China produces every year is at least
There are 4,000,000 t.The traditional method for processing these bacteria residues is to abandon or burn, and this not only causes the waste of agriculture organic resource, together
When also result in environmental pollution.How rationally, oneself becomes Edible Fungi Industry Development and environment guarantor scientifically to process edible fungi residue
Shield needs the common problem for solving.
The content of the invention
The purpose of the present invention is：The bacteria residue produced during Lentnus edodes is recycled, the system by way of fermentation
Standby to obtain fermented feed, the feed applications can reduce the fat in gift tilapia meat in gift tilapia breeding process
Content simultaneously improves mouthfeel.
A first aspect of the present invention：
A kind of Lentinus Edodes fungus residue fermented feed, it be by Lentinus Edodess bacteria residue and dregs of rice class, plant amylum after bacterium solution fermentation process, then
Compound vitamin is composited.
The mixing of one or two kinds of of the described dregs of rice class in bean cake, cottonseed meal.
Described plant amylum is a kind of or several in corn starch, soybean starch, potato starch, tapioca
The mixing planted.
Described compound vitamin selected from vitamin A, vitamin B, vitamin C, vitamin D, the one kind in Vitamin E or
Several mixing of person.
The preparation method of described Lentinus Edodes fungus residue fermented feed, comprises the steps：
1st step, in parts by weight, takes Lentinus Edodess 55～65 parts of bacteria residue of plantation, crushes, add the water of 5～8 times of weight, add egg
2～4 parts of 3～7 parts of white enzyme and cellulase, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；
2nd step, is 20～30% by the volume concentration of enzymolysis solution, then adds 20～35 parts of dregs of rice class, plant amylum 15～20 wherein
Part, adding 1～3 part of microbial inoculum, mix homogeneously is fermented after cover film after accumulation again, after fermentation ends, then
Dry in the sun；
3rd step, adds compound vitamin in the 2nd step in the fermented feed for obtaining, addition is the 0.00005 of fermented feed weight
～0.0001%, after mix homogeneously, obtain Lentinus Edodes fungus residue fermented feed.
In the 2nd described step, 30～40 DEG C of fermentation temperature, fermentation time 20～30 days.
In the 2nd described step, dry in the sun to moisture is 10～15wt%.
In the 2nd described step, microbial inoculum selected from bacillus subtilises bacterium solution, actinomycetes bacterium solution, yeast bacterium solution or
Person's bacillus bifiduss bacterium solution.
In the 3rd described step, compound vitamin is that vitamin B compares 1 according to weight with vitamin C：2～4 mixture.
Improve the method for breeding of gift tilapia meat, fed using above-mentioned Lentinus Edodes fungus residue fermented feed, feed beginning
When, the age of a fish of gift tilapia at 2～6 months, Feeding time at least 60 days.
Beneficial effect
The present invention can effectively make albumen, Plant fiber for wherein containing etc. by carrying out to Lentinus Edodess bacteria residue after fermentation process
Decomposed, more absorbed using gift tilapia, nutritional labeling therein is suitable for the digestion of gift tilapia, is absorbed so as to
Meat is more delicate, and crude fibre is less, improves the mouthfeel of gift tilapia, while the fat that can also be reduced in meat contains
Amount, more using edible health.
Specific embodiment
The Lentinus Edodes fungus residue fermented feed of the present invention be by Lentinus Edodess bacteria residue and dregs of rice class, plant amylum through bacterium solution fermentation process it
Afterwards, then compound vitamin is composited.Bacterium solution used herein above can be following several：
（1）The preparation of bacillus subtilises bacterium solution：
Aseptically, bacillus subtilises (Bacillus subtilis) CICC 23659 is linked in culture medium,
Liquid fermentation production is carried out, wherein described culture medium percentage is：Starch 5%, potassium humate 1%, soybean cake powder
7.5%th, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, magnesium sulfate 0.05%, ferric chloride 0.005%, Calcium Carbonate 0.05%, yeast powder
0.01%th, boric acid 0.005%, pH value are adjusted to 7.2～7.4, and 20 min that sterilize are carried out at 120 DEG C or so；
In sweat, 28～40 DEG C of cultivation temperature scope, are passed through filtrated air（Dissolved oxygen）And stir, the ventilation of filtrated air
0.5～1.0L ∕ Lmin, 150～200rpm of mixing speed, tank pressure 0.5kg ∕ cm2, oxygen consumption culture 48 hours, the bacterium solution for obtaining
Middle bacillus subtilises Si Shi subspecies bacterium number >=2.0 × 109cfu/ mL；
（2）The preparation of actinomycetes bacterium solution
Aseptically, by Cortex Acanthopanacis Radicis streptomycete（Streptomyces albospinus）CGMCC 7434 is inoculated into culture medium
In, liquid fermentation production is carried out, wherein described culture medium percentage is：Glucose 2.9%, 1.6 % of analysis for soybean powder,
Soluble starch 4%, yeast powder 1%, potassium humate 1%, 0.4 % of peptone, 0.05 0.2 % of %, NaCl of magnesium sulfate, carbonic acid
Calcium 0.5%, pH value is adjusted to 7.5～8.0, and 20 min that sterilize are carried out at 120 DEG C or so；
In sweat, 28～30 DEG C of cultivation temperature scope, are passed through filtrated air（Dissolved oxygen）And stir, the intake 2 of filtrated air
～3L ∕ Lmin, mixing speed are 150 ~ 210 rpm, and fermentation time obtains actinomycetes bacterium solution fermentation liquid for 60 ~ 130 hours, are fermented
Later stage, mycelia was all broken into the colony-forming units of the bacterium, colony-forming units >=1.5 × 10 of the bacterium in fermentation liquid9
cfu/ mL；
（3）The preparation of yeast bacterium solution
Aseptically, Candida utilis (Candida utilis) CICC 1422 is inoculated in culture medium, is carried out
Liquid fermentation is produced, wherein described culture medium is routine YPD yeast culture mediums；
In sweat, cultivation temperature is 25～40 DEG C, is passed through filtrated air（Dissolved oxygen）And stir, ventilation is 8～10L/
It is 200～300 rpm that Lmin, fermentation time are 36～72h, speed of agitator, the colony-forming units of the bacterium in fermentation liquid >=
1.0×109cfu/ mL；
（4）The preparation of bacillus bifiduss bacterium solution：
Aseptically, by bacillus bifiduss（Lactococcus lactis）ACCC 11054 is inoculated in fluid medium,
Wherein described culture medium percentage is：Fructus Lycopersici esculenti juice 20%, soluble starch 0.05%, peptone 1.5%, Sodium Chloride
5.0g, yeast 0.6%, glucose 2%, pH value is adjusted to 7.2；
In sweat, 30 DEG C～35 DEG C of cultivation temperature scope is cultivated 2～3 days, obtains the seed of bacterium number >=5.0 × 109cfu/ml
Culture fluid, is then seeded into 50 liters and contains 5%（Percetage by weight）In the sterilized water of brown sugar, inoculum concentration for sterilized water weight 2%,
Under conditions of in dissolved oxygen amount is 5% i.e. fermentation tank, the volume ratio of the volume of oxygen and fermentation liquid is 5%, 25 DEG C～30 DEG C in sealing
In tank, quiescent culture 10～15 days, obtain bacterium number >=1.0 × 109The bacillus bifiduss bacterium solution of cfu/ml.
Embodiment 1
The preparation method of Lentinus Edodes fungus residue fermented feed, comprises the steps：
1st step, in parts by weight, takes Lentinus Edodess 55 parts of bacteria residue of plantation, crushes, add the water of 5 times of weight, add protease 3 part
With 2 parts of cellulase, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；
2nd step, is 20% by the volume concentration of enzymolysis solution, then adds dregs of rice class wherein（Bean cake and cottonseed meal compare 1 according to weight：1
Mixture）20 parts, 15 parts of potato starch, add 1 part of bacillus subtilises bacterium solution, and mix homogeneously is covered after accumulation again
Fermented after lid thin film, 30 DEG C of fermentation temperature, fermentation time 20 days, after fermentation ends, then dry in the sun, dry in the sun to moisture is
10wt%；
3rd step, adds compound vitamin in the fermented feed for obtaining in the 2nd step（Vitamin B compares 1 according to weight with vitamin C：2
Mixture）, addition is the 0.00005% of fermented feed weight, after mix homogeneously, obtains Lentinus Edodes fungus residue fermented feed.
Embodiment 2
The preparation method of Lentinus Edodes fungus residue fermented feed, comprises the steps：
1st step, in parts by weight, takes Lentinus Edodess 65 parts of bacteria residue of plantation, crushes, add the water of 8 times of weight, add 7 parts of protease
With 4 parts of cellulase, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；
2nd step, is 30% by the volume concentration of enzymolysis solution, then adds dregs of rice class wherein（Bean cake and cottonseed meal compare 1 according to weight：1
Mixture）35 parts, 20 parts of potato starch, add 3 parts of bacillus subtilises bacterium solution, and mix homogeneously is covered after accumulation again
Fermented after lid thin film, 40 DEG C of fermentation temperature, fermentation time 30 days, after fermentation ends, then dry in the sun, dry in the sun to moisture is
15wt%；
3rd step, adds compound vitamin in the fermented feed for obtaining in the 2nd step（Vitamin B compares 1 according to weight with vitamin C：
4 mixture）, addition is the 0.0001% of fermented feed weight, after mix homogeneously, obtains Lentinus Edodes fungus residue fermented feed.
Embodiment 3
The preparation method of Lentinus Edodes fungus residue fermented feed, comprises the steps：
1st step, in parts by weight, takes Lentinus Edodess 60 parts of bacteria residue of plantation, crushes, add the water of 7 times of weight, add 6 parts of protease
With 3 parts of cellulase, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；
2nd step, is 25% by the volume concentration of enzymolysis solution, then adds dregs of rice class wherein（Bean cake and cottonseed meal compare 1 according to weight：1
Mixture）25 parts, 17 parts of potato starch, add 2 parts of bacillus subtilises bacterium solution, and mix homogeneously is covered after accumulation again
Fermented after lid thin film, 35 DEG C of fermentation temperature, fermentation time 25 days, after fermentation ends, then dry in the sun, dry in the sun to moisture is
12wt%；
3rd step, adds compound vitamin in the fermented feed for obtaining in the 2nd step（Vitamin B compares 1 according to weight with vitamin C：3
Mixture）, addition is the 0.00007% of fermented feed weight, after mix homogeneously, obtains Lentinus Edodes fungus residue fermented feed.
Reference examples 1
Difference with embodiment 3 is：Lentinus Edodess plant bacteria residue only through enzymolysis processing, without everfermentation.
1st step, in parts by weight, takes Lentinus Edodess 60 parts of bacteria residue of plantation, crushes, add the water of 7 times of weight, add protease
6 parts and 3 parts of cellulase, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；
2nd step, is 25% by the volume concentration of enzymolysis solution, then adds dregs of rice class wherein（Bean cake and cottonseed meal compare 1 according to weight：1
Mixture）25 parts, 17 parts of potato starch, mix homogeneously, then dry in the sun, dry in the sun to moisture is 12wt%；
3rd step, adds compound vitamin in the feedstuff for obtaining in the 2nd step（Vitamin B compares 1 according to weight with vitamin C：3 it is mixed
Compound）, addition is the 0.00007% of fermented feed weight, after mix homogeneously, obtains Lentinus Edodess mushroom dreg feed.
Reference examples 2
Cultivated using conventional commercial aquatic fish universal feed.
Carry out feeding test using the feedstuff prepared in above-described embodiment and reference examples, be respectively directed to gift tilapia
The age of a fish fish pond of 4 months and 8 months fed, Feeding time is 70 days.After feeding end, fish 20 is taken respectively in fish pond
Tail is detected, is averaged, as a result as follows：
As can be seen from the table, after feedstuff of the invention is fed for the gift tilapia at 4 monthly ages, its fish can be significantly increased
Protein content in meat simultaneously can reduce its fat content, better than unfermentable mushroom dreg feed.
As can be seen from the table, after feedstuff of the invention is fed for the gift tilapia at 4 monthly ages, can significantly decrease
Meat fiber diameter, makes meat more delicate, better than unfermentable mushroom dreg feed.
Improve the method for breeding of gift tilapia meat, fed using above-mentioned Lentinus Edodes fungus residue fermented feed, feed beginning
When, the age of a fish of gift tilapia at 2～6 months, Feeding time at least 60 days.
Claims (9)
1. a kind of Lentinus Edodes fungus residue fermented feed, it is characterised in that it is sent out through bacterium solution with dregs of rice class, plant amylum by Lentinus Edodess bacteria residue
After ferment process, then compound vitamin is composited.
2. Lentinus Edodes fungus residue fermented feed according to claim 1, it is characterised in that described dregs of rice class is selected from bean cake, Semen Gossypii
The mixing of the one or two kinds of in the dregs of rice.
3. Lentinus Edodes fungus residue fermented feed according to claim 1, it is characterised in that described plant amylum forms sediment selected from Semen Maydiss
One or several mixing in powder, soybean starch, potato starch, tapioca.
4. Lentinus Edodes fungus residue fermented feed according to claim 1, it is characterised in that described compound vitamin is selected from dimension life
One or several mixing in plain A, vitamin B, vitamin C, vitamin D, Vitamin E.
5. the preparation method of the Lentinus Edodes fungus residue fermented feed described in claim 1, comprises the steps：1st step, with weight portion
Meter, takes Lentinus Edodess 55～65 parts of bacteria residue of plantation, crushes, add the water of 5～8 times of weight, add protease 3～7 part and cellulose
2～4 parts of enzyme, heating enzymolysis, then enzyme denaturing, lets cool, and obtains enzymolysis solution；2nd step, is 20～30% by the volume concentration of enzymolysis solution,
Add 20～35 parts of dregs of rice class, 15～20 parts of plant amylum again wherein, add 1～3 part of microbial inoculum, mix homogeneously, heap
Fermented after cover film after product again, after fermentation ends, then dry in the sun；3rd step, adds in the fermented feed for obtaining in the 2nd step
Enter compound vitamin, addition is the 0.00005～0.0001% of fermented feed weight, after mix homogeneously, obtain Lentinus Edodess bacteria residue and send out
Ferment feedstuff.
6. Lentinus Edodes fungus residue fermented feed according to claim 5, it is characterised in that in the 2nd described step, fermentation temperature 30
～40 DEG C, fermentation time 20～30 days.
7. Lentinus Edodes fungus residue fermented feed according to claim 5, it is characterised in that in the 2nd described step, dry in the sun is to moisture
Content is 10～15wt%；Microbial inoculum is selected from bacillus subtilises bacterium solution, actinomycetes bacterium solution, yeast bacterium solution or bifid
Bacillus bacterium solution.
8. Lentinus Edodes fungus residue fermented feed according to claim 5, it is characterised in that in the 3rd described step, compound vitamin
It is that vitamin B compares 1 according to weight with vitamin C：2～4 mixture.
9. a kind of method for breeding for improving gift tilapia meat, it is characterised in that fermented using the Lentinus Edodess bacteria residue of claim 1
Feedstuff feeding, when feeding beginning, the age of a fish of gift tilapia at 2～6 months, Feeding time at least 60 days.
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| CN107484928A (en) * | 2017-09-27 | 2017-12-19 | 汾西县云城旭日特色养殖专业合作社 | A kind of Yellow meal worm larva feed and preparation method thereof |
| CN107836565A (en) * | 2017-11-24 | 2018-03-27 | 马鞍山市五谷禽业专业合作社 | A kind of production method of soybean solid-state fermentation thalli protein feed |
| CN108450641A (en) * | 2017-12-29 | 2018-08-28 | 青岛百瑞吉生物工程有限公司 | A method of preparing protein feed using edible fungi residue |
| CN110663810A (en) * | 2019-08-05 | 2020-01-10 | 佛山科学技术学院 | Biological fermentation feed and preparation method thereof |
| CN115316501A (en) * | 2022-08-16 | 2022-11-11 | 仲恺农业工程学院 | Application of mushroom leftover fermentation product as feed additive for micropterus salmoides |
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Cited By (5)
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| CN107484928A (en) * | 2017-09-27 | 2017-12-19 | 汾西县云城旭日特色养殖专业合作社 | A kind of Yellow meal worm larva feed and preparation method thereof |
| CN107836565A (en) * | 2017-11-24 | 2018-03-27 | 马鞍山市五谷禽业专业合作社 | A kind of production method of soybean solid-state fermentation thalli protein feed |
| CN108450641A (en) * | 2017-12-29 | 2018-08-28 | 青岛百瑞吉生物工程有限公司 | A method of preparing protein feed using edible fungi residue |
| CN110663810A (en) * | 2019-08-05 | 2020-01-10 | 佛山科学技术学院 | Biological fermentation feed and preparation method thereof |
| CN115316501A (en) * | 2022-08-16 | 2022-11-11 | 仲恺农业工程学院 | Application of mushroom leftover fermentation product as feed additive for micropterus salmoides |
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