CN108450641A - A method of preparing protein feed using edible fungi residue - Google Patents
A method of preparing protein feed using edible fungi residue Download PDFInfo
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- CN108450641A CN108450641A CN201810112095.4A CN201810112095A CN108450641A CN 108450641 A CN108450641 A CN 108450641A CN 201810112095 A CN201810112095 A CN 201810112095A CN 108450641 A CN108450641 A CN 108450641A
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- Prior art keywords
- edible fungi
- fungi residue
- protein feed
- preparing protein
- fermentation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
Abstract
The present invention provides a kind of methods preparing protein feed using edible fungi residue, first edible fungi residue are taken to be mixed with sodium hydroxide solution, are pre-processed；Complex enzyme is added in pretreatment fluid, is digested, prebiotic bacterium solution is added after high temperature enzyme deactivation in enzymolysis liquid, is sealed by fermentation, the mixture after fermentation is dry, crush to get；The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme；The prebiotic bacterium solution is made of aspergillus niger, candida tropicalis and bacillus subtilis.The present invention first pre-processes bacteria residue using sodium hydroxide solution, destroy the wood fibre of bacteria residue, complex enzyme is added to be digested, further destroy the fibre structure of bacteria residue, it finally uses the Mixed Microbes of aspergillus niger, candida tropicalis and bacillus subtilis composition to be converted, substantially increases protein content.
Description
Technical field
The invention belongs to technique for producing feed fields, and in particular to a kind of side preparing protein feed using edible fungi residue
Method.
Background technology
Edible fungi residue is that edible fungus culturing harvests remaining culture medium waste after product in the process, and China is edible mushroom life
Big country is produced, according to statistics, national edible mushroom total output accounts for 70% or more of global total output up to 1.83 × 107t within 2008.It is adjoint
The development of mushroom industry, has a large amount of edible fungi residue to generate every year, is calculated by edible fungus living being efficiency average 40%,
National edible fungi residue total output about 4.57 × 107t in 2008, enormous amount.However it is effective how to carry out environmental protection to bacteria residue
Processing is but never well solved.Annual a large amount of bacteria residue and useless side are banked up on the spot, or directly apply Tanaka,
On the one hand, the huge waste of agriculture organic resource is caused；On the other hand, the deterioration for causing ambient enviroment causes bacterium numerous
It grows, transmission, causes significant damage to neighbouring resident living environment, also cause and seriously affect to mushroom industry.Mesh
The preceding research on utilization to edible fungi residue, has focused largely on single field and research is not thorough enough, and bacteria residue cannot integrate, effectively
It utilizes.Therefore, how environmental protection effectively handles edible fungi residue well as an increasingly severeer problem.
According to analysis, the protein content and fructification in edible fungus cluster root are suitable, or even also height, belong to good protein
Resource, and edible fungus cluster root is also rich in the tryptophan lacked in vegetable protein.
Existing protein feed manufacturing technology is all to make protein feed using raw materials such as bone meal, fish meal, dregs of beans.Due to
Bone meal, fish meal, the dregs of beans market price are expensive, and production cost is high, and therefore, research direction is gradually aimed at environmental protection, efficient, nothing by people
Poison ferments to extract vegetable protein, makes protein feed to substitute original bone meal, fish meal, dregs of beans etc., reduces production cost.
Invention content
The object of the present invention is to provide a kind of methods preparing protein feed using edible fungi residue.
A method of protein feed being prepared using edible fungi residue, is included the following steps：
Step 1, edible fungi residue is taken to be mixed with 2-4wt% sodium hydroxide solutions, solid-liquid ratio 1：3-5 is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation；
Step 4, mixture after step 3 being fermented is dry, crush to get；
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme；
The prebiotic bacterium solution is made of aspergillus niger, candida tropicalis and bacillus subtilis.
Further, enzymatic hydrolysis condition is 45-50 DEG C in step 1.
Further, the weight ratio of the cellulase, dextranase, pectase and lignoenzyme is 2-4：1-2：1-2：
1-2。
Further, aspergillus niger, candida tropicalis and the weight ratio of bacillus subtilis are 1：1：1.
Further, the probiotics bacterial liquid be aspergillus niger, candida tropicalis and bacillus subtilis are seeded in it is red
Fermentation is made in sugar culture-medium liquid.
Further, brown sugar liquid medium be mass concentration be 20-30wt.% brown sugar aqueous solution, inoculum concentration 1wt.%,
Fermentation condition is 30-35 DEG C, 20-30h.
Further, fermentation condition is 15 DEG C -30 DEG C in step 3.
The present invention first pre-processes bacteria residue using sodium hydroxide solution, destroys the wood fibre of bacteria residue, adds multiple
Synthase is digested, and the fibre structure of bacteria residue is further destroyed, and finally uses aspergillus niger, candida tropicalis and bacillus subtilis
The Mixed Microbes of bacterium composition are converted, and protein content is substantially increased.
Specific implementation mode
The technical solution of invention is further illustrated with reference to specific embodiment.
Embodiment 1
A method of protein feed being prepared using edible fungi residue, is included the following steps：
Step 1, edible fungi residue is taken to be mixed with 2wt% sodium hydroxide solutions, solid-liquid ratio 1：5, it is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, hydrolysis temperature is 45 DEG C, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation, fermentation temperature is 15 DEG C；
Step 4, mixture after step 3 being fermented is dry, crush to get.
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme, and cellulase, Portugal are poly-
The weight ratio of carbohydrase, pectase and lignoenzyme is 2：1：1：1.
The probiotics bacterial liquid is that aspergillus niger, candida tropicalis and bacillus subtilis are seeded in brown sugar liquid medium
Middle fermentation is made, and brown sugar liquid medium is the brown sugar aqueous solution that mass concentration is 20wt.%, inoculum concentration 1wt.%, fermentation condition
For 30 DEG C, 30h.
The protein content of gained feed is 15%.
By gained feed for feeding poultry, it is layer of green-shell egg to produce layer breed, and quantity is 500, and nursing time is
12 months.For total egg production 142100, every equal egg production is 284.2.
Embodiment 2
A method of protein feed being prepared using edible fungi residue, is included the following steps：
Step 1, edible fungi residue is taken to be mixed with 3wt% sodium hydroxide solutions, solid-liquid ratio 1：4, it is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, hydrolysis temperature is 50 DEG C, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation, fermentation temperature is 25 DEG C；
Step 4, mixture after step 3 being fermented is dry, crush to get.
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme, and cellulase, Portugal are poly-
The weight ratio of carbohydrase, pectase and lignoenzyme is 3：2：1：2.
The probiotics bacterial liquid is that aspergillus niger, candida tropicalis and bacillus subtilis are seeded in brown sugar liquid medium
Middle fermentation is made, and brown sugar liquid medium is the brown sugar aqueous solution that mass concentration is 25wt.%, inoculum concentration 1wt.%, fermentation condition
For 35 DEG C, 20h.
The protein content of gained feed is 17%.
By gained feed for feeding poultry, it is layer of green-shell egg to produce layer breed, and quantity is 500, and nursing time is
12 months.For total egg production 144150, every equal egg production is 288.3.
Embodiment 3
A method of protein feed being prepared using edible fungi residue, is included the following steps：
Step 1, edible fungi residue is taken to be mixed with 4wt% sodium hydroxide solutions, solid-liquid ratio 1：3, it is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, hydrolysis temperature is 45 DEG C, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation, fermentation temperature is 20 DEG C；
Step 4, mixture after step 3 being fermented is dry, crush to get.
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme, and cellulase, Portugal are poly-
The weight ratio of carbohydrase, pectase and lignoenzyme is 4：1：2：1.
The probiotics bacterial liquid is that aspergillus niger, candida tropicalis and bacillus subtilis are seeded in brown sugar liquid medium
Middle fermentation is made, and brown sugar liquid medium is the brown sugar aqueous solution that mass concentration is 25wt.%, inoculum concentration 1wt.%, fermentation condition
For 30 DEG C, 20h.
The protein content of gained feed is 16%.
By gained feed for feeding poultry, it is layer of green-shell egg to produce layer breed, and quantity is 500, and nursing time is
12 months.For total egg production 145235, every equal egg production is 290.5.
Embodiment 4
A method of protein feed being prepared using edible fungi residue, is included the following steps：
Step 1, edible fungi residue is taken to be mixed with 4wt% sodium hydroxide solutions, solid-liquid ratio 1：4, it is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, hydrolysis temperature is 45 DEG C, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation, fermentation temperature is 15 DEG C；
Step 4, mixture after step 3 being fermented is dry, crush to get.
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme, and cellulase, Portugal are poly-
The weight ratio of carbohydrase, pectase and lignoenzyme is 3：2：2：1.
The probiotics bacterial liquid is that aspergillus niger, candida tropicalis and bacillus subtilis are seeded in brown sugar liquid medium
Middle fermentation is made, and brown sugar liquid medium is the brown sugar aqueous solution that mass concentration is 20wt.%, inoculum concentration 1wt.%, fermentation condition
For 30 DEG C, 30h.
The protein content of gained feed is 18%.
By gained feed for feeding poultry, it is layer of green-shell egg to produce layer breed, and quantity is 500, and nursing time is
12 months.For total egg production 145235, every equal egg production is 289.1.
Claims (7)
1. a kind of method preparing protein feed using edible fungi residue, it is characterised in that：Include the following steps：
Step 1, edible fungi residue is taken to be mixed with 2-4wt% sodium hydroxide solutions, solid-liquid ratio 1：3-5 is pre-processed；
Step 2, complex enzyme is added in the pretreatment fluid of step 1, is digested, high temperature enzyme deactivation；
Step 3, prebiotic bacterium solution is added in the enzymolysis liquid of step 2, is sealed by fermentation；
Step 4, mixture after step 3 being fermented is dry, crush to get；
The complex enzyme is the mixed enzyme of cellulase, dextranase, pectase and lignoenzyme；
The prebiotic bacterium solution is made of aspergillus niger, candida tropicalis and bacillus subtilis.
2. the method according to claim 1 for preparing protein feed using edible fungi residue, it is characterised in that：In step 2
Enzymatic hydrolysis condition is 45-50 DEG C.
3. the method according to claim 1 for preparing protein feed using edible fungi residue, it is characterised in that：The fiber
Plain enzyme, dextranase, pectase and lignoenzyme weight ratio be 2-4：1-2：1-2：1-2.
4. the method according to claim 1 for preparing protein feed using edible fungi residue, it is characterised in that：Aspergillus niger,
The weight ratio of candida tropicalis and bacillus subtilis is 1：1：1.
5. the method according to claim 1 for preparing protein feed using edible fungi residue, it is characterised in that：It is described prebiotic
Bacterium bacterium solution is that aspergillus niger, candida tropicalis and bacillus subtilis are seeded in fermentation in brown sugar liquid medium to be made.
6. the method according to claim 5 for preparing protein feed using edible fungi residue, it is characterised in that：Brown sugar culture
Base fluid is the brown sugar aqueous solution that mass concentration is 20-30wt.%, and inoculum concentration 1wt.%, fermentation condition is 30-35 DEG C, 20-30h.
7. the method according to claim 1 for preparing protein feed using edible fungi residue, it is characterised in that：In step 3
Fermentation condition is 15 DEG C -30 DEG C.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711478969X | 2017-12-29 | ||
| CN201711478969 | 2017-12-29 |
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| CN108450641A true CN108450641A (en) | 2018-08-28 |
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| CN201810112095.4A Withdrawn CN108450641A (en) | 2017-12-29 | 2018-02-05 | A method of preparing protein feed using edible fungi residue |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349285A (en) * | 2021-04-28 | 2021-09-07 | 天津纳尔生物科技有限公司 | Microbial fermentation feed additive and production method thereof |
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|---|---|---|---|---|
| CN102613388A (en) * | 2011-01-30 | 2012-08-01 | 俸祥仁 | Technology for mix-fermenting edible fungal residues into forage by microbes and enzyme preparation |
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN104171759A (en) * | 2014-09-09 | 2014-12-03 | 中国科学院天津工业生物技术研究所 | Edible mushroom residue feed preparation process |
| CN105076715A (en) * | 2015-02-14 | 2015-11-25 | 裴学熠 | Microecological feed additive fermentation production method by using waste dross for enzymolysis of multiple bacteria |
| CN105661030A (en) * | 2016-01-25 | 2016-06-15 | 刘景阳 | Method for producing fodder from edible fungus residues by means of mixed fermentation by aid of microorganisms and enzyme preparations |
| CN106578723A (en) * | 2016-12-17 | 2017-04-26 | 王先涛 | Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia |
-
2018
- 2018-02-05 CN CN201810112095.4A patent/CN108450641A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613388A (en) * | 2011-01-30 | 2012-08-01 | 俸祥仁 | Technology for mix-fermenting edible fungal residues into forage by microbes and enzyme preparation |
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN104171759A (en) * | 2014-09-09 | 2014-12-03 | 中国科学院天津工业生物技术研究所 | Edible mushroom residue feed preparation process |
| CN105076715A (en) * | 2015-02-14 | 2015-11-25 | 裴学熠 | Microecological feed additive fermentation production method by using waste dross for enzymolysis of multiple bacteria |
| CN105661030A (en) * | 2016-01-25 | 2016-06-15 | 刘景阳 | Method for producing fodder from edible fungus residues by means of mixed fermentation by aid of microorganisms and enzyme preparations |
| CN106578723A (en) * | 2016-12-17 | 2017-04-26 | 王先涛 | Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113349285A (en) * | 2021-04-28 | 2021-09-07 | 天津纳尔生物科技有限公司 | Microbial fermentation feed additive and production method thereof |
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