CN107836565A - A kind of production method of soybean solid-state fermentation thalli protein feed - Google Patents
A kind of production method of soybean solid-state fermentation thalli protein feed Download PDFInfo
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- CN107836565A CN107836565A CN201711186882.5A CN201711186882A CN107836565A CN 107836565 A CN107836565 A CN 107836565A CN 201711186882 A CN201711186882 A CN 201711186882A CN 107836565 A CN107836565 A CN 107836565A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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Abstract
A kind of production method of soybean solid-state fermentation thalli protein feed, it is characterised in that it includes, the extraction of lentinan, the activation of saccharomycete are cultivated with expanding, soybean removal of impurities cleaning dried and crushed, using the bacteria residue of soy meal and extraction lentinan as raw material by solid fermentation, airless spraying lentinan.The present invention uses the bacteria residue using soy meal and extraction lentinan to prepare protein feed as raw material by solid fermentation, has culture medium simple and wide material sources, production efficiency are high, fermentation residue processing is simple, low input, low power consuming, the advantages that low technical ability, it is easy to promote, feasibility is strong.
Description
Technical field
The invention belongs to field of fodder, and in particular to a kind of production method of soybean solid-state fermentation thalli protein feed.
Background technology
With the rapid development of economy, living standards of the people increasingly improve, so as to promote need of the people to livestock products
The amount of asking greatly improves, and causes China to increase the demand of feed year by year, the situation that supply-demand relationship is difficult to balance occurs.Especially
It is the present situation of in the market protein feed raw material shortage, hardly possible has met the fast-developing demand of animal husbandry.
The content of the invention
It is an object of the invention to provide a kind of production method of the soybean solid-state fermentation thalli protein feed of high protein.
In order to realize above-mentioned purpose, present invention employs following technical scheme：
A kind of production method of soybean solid-state fermentation thalli protein feed, it is characterised in that it includes, the extraction of lentinan,
The activation of saccharomycete is cultivated with expanding, soybean removal of impurities cleaning dried and crushed, using the bacteria residue of soy meal and extraction lentinan as original
Expect solid state fermentation, airless spraying lentinan.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that it is concretely comprised the following steps：
（1）Mushroom is cleaned 2-3 times, natural air drying, attrition grinding 50-60min, cross 80-100 mesh sieves, obtain mushroom powder；By perfume (or spice)
Mushroom powder is added in 75% ethanol water of 10-12 times of parts by weight, 70-75 DEG C of return stirring 2-3h, is centrifuged, and filtering, stays filter
Slag；Filter residue is added in the deionized water of 15-18 times of parts by weight, microwave power 400-500W, radiated time 50-
60min, filtering, collect extract solution and precipitation；
（2）To step（1）In the extract solution of gained, the enzyme of 0.015-0.022 times of parts by weight is added, regulation system pH is 4.5-5,
55-60 DEG C of temperature constant magnetic stirring digests 50-60min, is brought rapidly up to 95-100 DEG C, enzyme deactivation 5-8min, centrifuges, obtains
Clear liquid；By supernatant decompression rotary evaporation concentration, the absolute ethyl alcohol equivalent to 4-5 times of parts by weight of concentrate, magnetic agitation are added
Alcohol precipitation 20-24h in 40-50min, 0-4 DEG C of refrigerator, centrifugation, obtain centrifuging thing；Centrifugation thing is added to 10-12 times of parts by weight
In deionized water, stirring is completely dissolved it, dialyses 2-3 days, freeze-drying, obtains lentinan；
（3）Soybean is removed into the impurity such as sandstone, cleaning, dried；The soybean cleaned after drying is added in beater disintegrating machine
Crush, the sieve plate sieve diameter of outfit is 1mm, obtains soy meal；
（4）Aseptically, barms is inoculated on potato dextrose agar, is placed in insulating box,
It is incubated at 30-35 DEG C, 72h is activated, obtains good quality strain；Aseptically, with 10mL aseptic water washing potatos Portugal
The strain activated on grape sugar agar medium, it is seeded to and expands in culture medium, be placed in shaking table, in 30-35 DEG C of condition
Under, 160-180r/min concussion and cultivate 72h, obtain the scale-up medium that culture is completed；
（5）The scale-up medium for cultivating completion is added in the mineral element nutrient solution of 4-5 times of parts by weight, after being sufficiently mixed,
The physiological saline of 4-5 times of parts by weight is added again；Soy meal and step are uniformly sprayed onto after stirring（1）The precipitation mixing of gained mixes
In compound, do not stop to stir, continuing addition physiological saline to soy meal is captured has moist feeling, has water to ooze out but do not drip after clutching
Under, soy meal can scatter without agglomerating automatically after loosing one's grip, and now material-water ratio is about 1：1.5-1.8；
（6）Soy meal after mixing is added in sealed bag, sealed after draining air, is placed at shady and cool lucifuge under natural conditions
Carry out fermentation 30-35d；Lentinan is dissolved in the distilled water of 8-10 times of parts by weight, then by lentinan solution airless spraying
Soy meal surface after fermentation, dries, and obtains soybean solid-state fermentation thalli protein feed.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that step（2）In enzyme for fibre
Plain enzyme, pectase, protease are tieed up with 1：0.8-0.9：0.7-0.8 mass ratio mixes.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that step（4）Middle potato Portugal
Grape sugar agar medium is：The potato 200g for removing the peel processing is cut into bulk, boils 30-40min in 1000mL distilled water,
Block potato is filtered off, obtains potato juice；Distilled water is added into potato juice and is settled to 1000mL, adds 15g grapes
Sugar and 20g agar, after being mixed evenly, 110-120 DEG C of sterilizing 25-30min, take out inclined-plane and place, cooling.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that step（4）It is middle to expand culture
Culture medium is：The potato 200g for removing the peel processing is cut into bulk, 30-40min is boiled in 1000mL distilled water, is filtered off block
Potato, obtain potato juice；Distilled water is added into potato juice and is settled to 1000mL, adds 15g glucose, mixing is stirred
After mixing uniformly, 110-120 DEG C of sterilizing 25-30min, take out inclined-plane and place, cooling.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that step（5）Mineral Elements
Nutrient solution：Ammonium sulfate 1.4g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 0.6g, calcium chloride 0.3g, sodium chloride 0.5g, ferric sulfate
0.05g, manganese sulfate 0.02g, zinc chloride 0.02g, cobalt chloride 0.005g, it is added in 1000mL distilled water, mixes to
Fully dissolving.
The production method of described soybean solid-state fermentation thalli protein feed, it is characterised in that step（6）Middle lentinan
Mass ratio with the soy meal after fermentation is 1：1000-1200.
By above-mentioned technical scheme, the beneficial effects of the invention are as follows：
The present invention by mushroom is cleaned, air-dried, crushing, removal of impurities, microwave radiation exaraction, enzymolysis, dialysis, freeze-drying, obtain perfume (or spice)
Mushroom polysaccharide；Using microwave radiation technology and collective effect is digested, improves the recovery rate of lentinan；The present invention will have it is antitumor,
The lentinan of the effect such as antiviral, immunological regulation, anti-oxidant, anticoagulation is sprayed on Zymosis soybean meal using airless spraying technology
Surface, the immunologic function of livestock is improved, add the disease resistance of livestock.The present invention uses with soy meal and extracts lentinan
Bacteria residue is that raw material by solid fermentation prepares protein feed, on the one hand, sends out the bacteria residue after discarded extraction lentinan by solid
Ferment, waste residue value is greatly promoted, it is turned waste into wealth, on the other hand, solid fermentation protein feed has culture medium simple and come
Source is extensive, and production efficiency is high, and fermentation residue processing is simple, low input, low power consuming, the advantages that low technical ability, is easy to promote, feasibility
By force.
Embodiment
The production method of the soybean solid-state fermentation thalli protein feed of the present embodiment, it is concretely comprised the following steps：
（1）Mushroom is cleaned 3 times, natural air drying, attrition grinding 60min, cross 100 mesh sieves, obtain mushroom powder；Mushroom powder is added
Into 75% ethanol water of 10 times of parts by weight, 75 DEG C of return stirring 2h, centrifuge, filtering, stay filter residue；Filter residue is added to
In the deionized water of 18 times of parts by weight, microwave power 500W, radiated time 60min, filtering, extract solution and precipitation are collected；
（2）To step（1）In the extract solution of gained, the enzyme of 0.015 times of parts by weight is added, regulation system pH is 5,60 DEG C of constant temperature magnetic
Power stirring enzymolysis 60min, is brought rapidly up to 100 DEG C, enzyme deactivation 8min, centrifuges, obtain supernatant；Supernatant is depressurized and rotated
It is concentrated by evaporation, adds the absolute ethyl alcohol equivalent to 5 times of parts by weight of concentrate, magnetic agitation 50min, alcohol precipitation in 4 DEG C of refrigerator
24h, centrifugation, obtain centrifuging thing；Centrifugation thing is added in the deionized water of 12 times of parts by weight, and stirring is completely dissolved it, dialysis 2
My god, freeze-drying, obtain lentinan；
（3）Soybean is removed into the impurity such as sandstone, cleaning, dried；The soybean cleaned after drying is added in beater disintegrating machine
Crush, the sieve plate sieve diameter of outfit is 1mm, obtains soy meal；
（4）Aseptically, barms is inoculated on potato dextrose agar, is placed in insulating box,
It is incubated at 35 DEG C, 72h is activated, obtains good quality strain；Aseptically, with 10mL aseptic water washing potato glucoses
The strain activated on agar medium, it is seeded to and expands in culture medium, be placed in shaking table, under the conditions of 35 DEG C, 180r/
Min concussion and cultivate 72h, obtain the scale-up medium that culture is completed；
（5）The scale-up medium for cultivating completion is added in the mineral element nutrient solution of 5 times of parts by weight, after being sufficiently mixed, then
Add the physiological saline of 5 times of parts by weight；Soy meal and step are uniformly sprayed onto after stirring（1）The mixture of the precipitation mixing of gained
In, do not stop to stir, continuing addition physiological saline to soy meal is captured has moist feeling, has water to ooze out but do not drip after clutching, pine
Soy meal can scatter without agglomerating automatically after hand, and now material-water ratio is about 1：1.8；
（6）Soy meal after mixing is added in sealed bag, sealed after draining air, is placed at shady and cool lucifuge under natural conditions
Carry out fermentation 35d；Lentinan is dissolved in the distilled water of 10 times of parts by weight, then the airless spraying of lentinan solution is being fermented
Soy meal surface afterwards, dries, and obtains soybean solid-state fermentation thalli protein feed.
The step of the present embodiment（2）In enzyme be cellulase, pectase, protease is with 1：0.9：0.8 mass ratio mixes
Conjunction forms.
The step of the present embodiment（4）Middle potato dextrose agar is：The potato 200g for removing the peel processing is cut
Into bulk, 30min is boiled in 1000mL distilled water, block potato is filtered off, obtains potato juice；Added into potato juice
Distilled water is settled to 1000mL, adds 15g glucose and 20g agar, after being mixed evenly, 120 DEG C of sterilizing 30min, takes
Go out inclined-plane placement, cooling.
The step of the present embodiment（4）It is middle expansion culture medium be：The potato 200g for removing the peel processing is cut into bulk,
30min is boiled in 1000mL distilled water, block potato is filtered off, obtains potato juice；Distilled water is added into potato juice to determine
Hold to 1000mL, add 15g glucose, after being mixed evenly, 120 DEG C of 30min that go out, take out inclined-plane and place, cooling.
The step of the present embodiment（5）Mineral Elements nutrient solution：Ammonium sulfate 1.4g, potassium dihydrogen phosphate 3.0g, magnesium sulfate
0.6g, calcium chloride 0.3g, sodium chloride 0.5g, ferric sulfate 0.05g, manganese sulfate 0.02g, zinc chloride 0.02g, cobalt chloride 0.005g,
It is added in 1000mL distilled water, mixes to abundant dissolving.
The step of the present embodiment（6）The mass ratio of middle lentinan and the soy meal after fermentation is 1：1000.
Claims (7)
1. a kind of production method of soybean solid-state fermentation thalli protein feed, it is characterised in that it includes, and lentinan carries
Take, the activation of saccharomycete is cultivated with expanding, soybean removal of impurities cleaning dried and crushed, using the bacteria residue of soy meal and extraction lentinan as
Raw material by solid fermentation, airless spraying lentinan.
2. the production method of the soybean solid-state fermentation thalli protein feed according to claims 1, it is characterised in that it has
Body step is：
（1）Mushroom is cleaned 2-3 times, natural air drying, attrition grinding 50-60min, cross 80-100 mesh sieves, obtain mushroom powder；By perfume (or spice)
Mushroom powder is added in 75% ethanol water of 10-12 times of parts by weight, 70-75 DEG C of return stirring 2-3h, is centrifuged, and filtering, stays filter
Slag；Filter residue is added in the deionized water of 15-18 times of parts by weight, microwave power 400-500W, radiated time 50-
60min, filtering, collect extract solution and precipitation；
（2）To step（1）In the extract solution of gained, the enzyme of 0.015-0.022 times of parts by weight is added, regulation system pH is 4.5-5,
55-60 DEG C of temperature constant magnetic stirring digests 50-60min, is brought rapidly up to 95-100 DEG C, enzyme deactivation 5-8min, centrifuges, obtains
Clear liquid；By supernatant decompression rotary evaporation concentration, the absolute ethyl alcohol equivalent to 4-5 times of parts by weight of concentrate, magnetic agitation are added
Alcohol precipitation 20-24h in 40-50min, 0-4 DEG C of refrigerator, centrifugation, obtain centrifuging thing；Centrifugation thing is added to 10-12 times of parts by weight
In deionized water, stirring is completely dissolved it, dialyses 2-3 days, freeze-drying, obtains lentinan；
（3）Soybean is removed into the impurity such as sandstone, cleaning, dried；The soybean cleaned after drying is added in beater disintegrating machine
Crush, the sieve plate sieve diameter of outfit is 1mm, obtains soy meal；
（4）Aseptically, barms is inoculated on potato dextrose agar, is placed in insulating box,
It is incubated at 30-35 DEG C, 72h is activated, obtains good quality strain；Aseptically, with 10mL aseptic water washing potatos Portugal
The strain activated on grape sugar agar medium, it is seeded to and expands in culture medium, be placed in shaking table, in 30-35 DEG C of condition
Under, 160-180r/min concussion and cultivate 72h, obtain the scale-up medium that culture is completed；
（5）The scale-up medium for cultivating completion is added in the mineral element nutrient solution of 4-5 times of parts by weight, after being sufficiently mixed,
The physiological saline of 4-5 times of parts by weight is added again；Soy meal and step are uniformly sprayed onto after stirring（1）The precipitation mixing of gained mixes
In compound, do not stop to stir, continuing addition physiological saline to soy meal is captured has moist feeling, has water to ooze out but do not drip after clutching
Under, soy meal can scatter without agglomerating automatically after loosing one's grip, and now material-water ratio is about 1：1.5-1.8；
（6）Soy meal after mixing is added in sealed bag, sealed after draining air, is placed at shady and cool lucifuge under natural conditions
Carry out fermentation 30-35d；Lentinan is dissolved in the distilled water of 8-10 times of parts by weight, then by lentinan solution airless spraying
Soy meal surface after fermentation, dries, and obtains soybean solid-state fermentation thalli protein feed.
3. the production method of the soybean solid-state fermentation thalli protein feed according to claims 2, it is characterised in that step
（2）In enzyme be cellulase, pectase, protease is with 1：0.8-0.9：0.7-0.8 mass ratio mixes.
4. the production method of the soybean solid-state fermentation thalli protein feed according to claims 2, it is characterised in that step
（4）Middle potato dextrose agar is：The potato 200g for removing the peel processing is cut into bulk, in 1000mL distilled water
30-40min is boiled, block potato is filtered off, obtains potato juice；Distilled water is added into potato juice and is settled to 1000mL,
15g glucose and 20g agar are added, after being mixed evenly, 110-120 DEG C of sterilizing 25-30min, inclined-plane is taken out and places, it is cold
But.
5. the production method of the soybean solid-state fermentation thalli protein feed according to claims 2, it is characterised in that step
（4）It is middle expansion culture medium be：The potato 200g for removing the peel processing is cut into bulk, boils 30- in 1000mL distilled water
40min, block potato is filtered off, obtains potato juice；Distilled water is added into potato juice and is settled to 1000mL, is added
15g glucose, after being mixed evenly, 110-120 DEG C of sterilizing 25-30min, take out inclined-plane and place, cooling.
6. the production method of the soybean solid-state fermentation thalli protein feed according to claims 2, it is characterised in that step
（5）Mineral Elements nutrient solution：Ammonium sulfate 1.4g, potassium dihydrogen phosphate 3.0g, magnesium sulfate 0.6g, calcium chloride 0.3g, sodium chloride
0.5g, ferric sulfate 0.05g, manganese sulfate 0.02g, zinc chloride 0.02g, cobalt chloride 0.005g, it is added in 1000mL distilled water,
Mix to abundant dissolving.
7. the production method of the soybean solid-state fermentation thalli protein feed according to claims 2, it is characterised in that step
（6）The mass ratio of middle lentinan and the soy meal after fermentation is 1：1000-1200.
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