CN104591835A - Preparation method of mushroom residue organic fertilizer for scutellaria baicalensis planting - Google Patents
Preparation method of mushroom residue organic fertilizer for scutellaria baicalensis planting Download PDFInfo
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Abstract
The invention relates to a preparation method of a mushroom residue organic fertilizer for scutellaria baicalensis planting and belongs to the technical field of organic fertilizers. The preparation method comprises the following steps: taking mushroom planting mushroom residues, crushing, adding water, adding protease and cellulose, heating, performing enzymatic hydrolysis, performing enzyme deactivation, chilling and obtaining an enzymatic hydrolysate; adding dried cow dung, maize straws, cottonseed cakes, humic acid, wheat bran, rice bran, soybean meal and saccharomycetes into the enzymatic hydrolysate, uniformly mixing, accumulating, covering a thin film, fermenting, airing and drying after the completion of fermentation and obtaining a fermented manure; uniformly mixing the fermented manure with diatomite, vermiculite, bentonite, urea, monoammonium phosphate, potassium sulphate, charcoal powder and superphosphate, mold-pressing and pelleting on a pelletizer and obtaining the organic fertilizer. According to the preparation method, through the recycling of the mushroom residues and the complex decomposition of the protease and the cellulose, after fermentation, the mushroom residue organic fertilizer is mixed with other fertilizers to prepare a compound fertilizer and can improve the output and the content of baicalin when being applied to the planting of the scutellaria baicalensis.
Description
Technical field
The present invention relates to a kind of preparation method of the bacterium residue organic fertilizer for root of large-flowered skullcap plantation, belong to organic fertilizer technical field.
Background technology
The root of large-flowered skullcap (Scutellaria baicalensis G) is conventional Chinese medicine.Commodity are mainly the root of the northern labiate root of large-flowered skullcap of China; In addition, Scutellaria still has the root of several plant also to do root of large-flowered skullcap use in some area.The root of large-flowered skullcap is mainly used in antibacterial, the effect such as anti-inflammatory and immunomodulatory, antipyretic-antalgic, Adjust-blood lipid and anti-AIDS.
Its pharmacological action is mainly as follows: one, anti-oxidant: the main active ingredient scutellarin of the root of large-flowered skullcap, baicalin energy scavenging hydroxyl, alkane free radical; the mitochondrial lipid peroxidation that suppression causes thus and the metabolism of Yelkin TTS plastid; to the cell injury that resistive to hydrogen peroxide causes, there is provide protection to myocardial ischemia-reperfusion.Two, anticarcinogenesis: scutellarin can suppress aflatoxin B1 and nitroguanidine to the mutagenesis of Salmonellas, can reduce the frequency that aflatoxin causes chromosome aberration, also can strengthen the cancer suppressing action of the cell medium such as interleukin and tumour necrosis factor.Three, antiviral and anti-microbial effect: baicalin, lower than can suppressor T cell and hiv virus during cytotoxic concentration, at the beginning of HIV-1 is by cell adsorption, can stop it to copy.Already proved, the Main Function link of baicalin is that interference HIV-1 attacks target cell, and being therefore expected to develop becomes new anti-AIDS drug.The pathomycete of baicalin to skin and nail also has restraining effect.Four, to the effect of blood vessel: lower concentration scutellarin presents contraction in vitro mesenteric artery, lax vascular smooth muscle effect during high density, is then presented.
The artificial culture root of large-flowered skullcap, use nitrogen, phosphorus, potassium 3 kinds of fertilizer, no matter use separately or compounding application, to the production-increasing function that the root of large-flowered skullcap all has, wherein the highest with the output of nitrogen, phosphorus, potassium 3 kinds of fertilizer compounding applications, 3 kinds of cooperations are greater than 2 kinds, 2 kinds are greater than a kind, being used alone a kind is the best with nitrogen again, and potassium takes second place, and phosphorus is worst.But life-time service chemical fertilizer also exists soil infringement, the impact being unfavorable for environmental protection, polluted underground water source.
Biological organic fertilizer refers to microorganisms with specific functions and is mainly originate and have the fertilizer of microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently with plant and animal residues (as feces of livestock and poultry, agricultural crop straw etc.).
Therefore, a kind of biological organic fertilizer that can be applied to root of large-flowered skullcap plantation is provided to have great importance.
Summary of the invention
The object of the invention is: a kind of bacterium residue organic fertilizer that the root of large-flowered skullcap can be made to plant raising output, raising content of baicalin is provided.
Technical scheme:
For a preparation method for the bacterium residue organic fertilizer of root of large-flowered skullcap plantation, comprise the steps:
1st step, by weight, gets mushroom plantation bacterium slag 70 ~ 90 parts, is crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 3 ~ 6 times of weight, then add 1 ~ 3 part, proteolytic enzyme and cellulase 1 ~ 3 part, heating, enzymolysis, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 40 ~ 50 parts, maize straw 30 ~ 50 parts, cottonseed cake 20 ~ 30 parts, humic acid 5 ~ 10 parts, 10 ~ 20 parts, wheat bran, 10 ~ 20 parts, rice bran, dregs of beans 10 ~ 20 parts, 1 ~ 3 part, yeast, mix, ferment after re-cover film after accumulation, after fermentation ends, airing to moisture content is 20 ~ 30wt% again, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with 5 ~ 10 parts, diatomite, vermiculite 5 ~ 10 parts, wilkinite 10 ~ 16 parts, 10 ~ 20 parts, urea, monoammonium phosphate 6 ~ 8 parts, potassium sulfate 6 ~ 8 parts, wooden carbon dust 10 ~ 15 parts, calcium superphosphate 5 ~ 12 parts after; in the granulation of tablets press top die, obtain fertilizer.
In the 1st described step, Heating temperature is 40 ~ 50 DEG C, and hydrolysis temperature is 3 ~ 6 hours.
In the 2nd described step, leavening temperature 30 ~ 40 DEG C, fermentation time 20 ~ 30 days.
beneficial effect
The present invention, by carrying out regeneration to Lentinus Edodes fungus slag, is decomposed by the compound of proteolytic enzyme and cellulase, more by fermentation after, then be mixed and made into composite fertilizer with other fertilizer, when being applied to root of large-flowered skullcap plantation, the content of output and baicalin can be improved.
Embodiment
embodiment 1
1st step, get mushroom plantation bacterium slag 70Kg, be crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 3 times of weight, then add proteolytic enzyme 1Kg and cellulase 1Kg, be heated to 40 DEG C, enzymolysis 3 hours, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 40Kg, maize straw 30Kg, cottonseed cake 20Kg, humic acid 5Kg, wheat bran 10Kg, rice bran 10Kg, dregs of beans 10Kg, yeast 1Kg, mix, ferment after re-cover film after accumulation, leavening temperature 30 DEG C, fermentation time 20 days, after fermentation ends, then airing to moisture content is 20wt%, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with diatomite 5Kg, vermiculite 5Kg, wilkinite 10Kg, urea 10Kg, monoammonium phosphate 6Kg, potassium sulfate 6Kg, wooden carbon dust 10Kg, calcium superphosphate 5Kg after, in the granulation of tablets press top die, obtain fertilizer.
embodiment 2
1st step, get mushroom plantation bacterium slag 90Kg, be crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 6 times of weight, then add protease 3 Kg and cellulase 3Kg, be heated to 50 DEG C, enzymolysis 6 hours, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 50Kg, maize straw 50Kg, cottonseed cake 30Kg, humic acid 10Kg, wheat bran 20Kg, rice bran 20Kg, dregs of beans 20Kg, yeast 3Kg, mix, ferment after re-cover film after accumulation, leavening temperature 40 DEG C, fermentation time 30 days, after fermentation ends, then airing to moisture content is 30wt%, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with diatomite 10Kg, vermiculite 10Kg, wilkinite 16Kg, urea 20Kg, monoammonium phosphate 8Kg, potassium sulfate 8Kg, wooden carbon dust 15Kg, calcium superphosphate 12Kg after, in the granulation of tablets press top die, obtain fertilizer.
embodiment 3
1st step, get mushroom plantation bacterium slag 80Kg, be crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 5 times of weight, then add proteolytic enzyme 2Kg and cellulase 2Kg, be heated to 45 DEG C, enzymolysis 5 hours, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 45Kg, maize straw 40Kg, cottonseed cake 25Kg, humic acid 8Kg, wheat bran 17Kg, rice bran 16Kg, dregs of beans 18Kg, yeast 2Kg, mix, ferment after re-cover film after accumulation, leavening temperature 36 DEG C, fermentation time 27 days, after fermentation ends, then airing to moisture content is 23wt%, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with diatomite 7Kg, vermiculite 8Kg, wilkinite 14Kg, urea 15Kg, monoammonium phosphate 7Kg, potassium sulfate 7Kg, wooden carbon dust 14Kg, calcium superphosphate 8Kg after, in the granulation of tablets press top die, obtain fertilizer.
reference examples 1
Be with the difference of embodiment 3: do not add proteolytic enzyme.
1st step, get mushroom plantation bacterium slag 80Kg, be crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 5 times of weight, then add cellulase 2Kg, be heated to 45 DEG C, enzymolysis 5 hours, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 45Kg, maize straw 40Kg, cottonseed cake 25Kg, humic acid 8Kg, wheat bran 17Kg, rice bran 16Kg, dregs of beans 18Kg, yeast 2Kg, mix, ferment after re-cover film after accumulation, leavening temperature 36 DEG C, fermentation time 27 days, after fermentation ends, then airing to moisture content is 23wt%, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with diatomite 7Kg, vermiculite 8Kg, wilkinite 14Kg, urea 15Kg, monoammonium phosphate 7Kg, potassium sulfate 7Kg, wooden carbon dust 14Kg, calcium superphosphate 8Kg after, in the granulation of tablets press top die, obtain fertilizer.
reference examples 2
Be with the difference of embodiment 3: do not add cellulase.
1st step, get mushroom plantation bacterium slag 80Kg, be crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 5 times of weight, then add proteolytic enzyme 2Kg, be heated to 45 DEG C, enzymolysis 5 hours, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 45Kg, maize straw 40Kg, cottonseed cake 25Kg, humic acid 8Kg, wheat bran 17Kg, rice bran 16Kg, dregs of beans 18Kg, yeast 2Kg, mix, ferment after re-cover film after accumulation, leavening temperature 36 DEG C, fermentation time 27 days, after fermentation ends, then airing to moisture content is 23wt%, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with diatomite 7Kg, vermiculite 8Kg, wilkinite 14Kg, urea 15Kg, monoammonium phosphate 7Kg, potassium sulfate 7Kg, wooden carbon dust 14Kg, calcium superphosphate 8Kg after, in the granulation of tablets press top die, obtain fertilizer.
performance test
Root of large-flowered skullcap planting experiment is carried out, greenhouse gardening, each test group area 30m in somewhere, Henan
2, spacing in the rows 20 ~ 30cm, every m
2fertilising 2Kg, applies fertilizer from during seedlings plugging in May then, to the seedling of late October then, investigates the surviving rate in whole seedling raising process, single plant yield, content of baicalin.
Wherein, the detection method of content of baicalin adopts HPLC chromatography determination.
Chromatographic condition: Agilent 1100 high performance liquid chromatograph; Chromatographic column: C
18; Moving phase: methanol-water-phosphoric acid (47:53:0.2); Determined wavelength: 280 nm; Flow velocity: 1.0 ml/min; Column temperature: 25 DEG C.
Prepared by reference substance solution: the baicalin of vacuum drier drying is made into 1ml and contains the solution of 60 μ g and get final product.
Prepared by need testing solution: get tetraploid Baical Skullcap Root (crossing 80 mesh sieves) about 0. 3g, accurately weighedly add 70% ethanol 40ml reflux 3h, let cool, filter, filtrate puts l00ml volumetric flask, with a small amount of 70% ethanol gradation washing container and residue, in the same volumetric flask of washing lotion filter people, add 70% ethanol and be settled to scale, the accurate 0.5ml. that draws adds in 5ml volumetric flask, add methanol constant volume to scale, shake up and get final product.
Accurate absorption trial-product, each 10 μ 1 of reference substance solution, inject high performance liquid chromatograph, and record peak area, calculates by external standard method, obtain the percentage composition of baicalin in dry product.
Table 1
| Average single plant yield g | Content of baicalin % | |
| Embodiment 1 | 21.5 | 10.22 |
| Embodiment 2 | 20.3 | 10.18 |
| Embodiment 3 | 22.4 | 10.88 |
| Reference examples 1 | 18.4 | 9.22 |
| Reference examples 2 | 18.1 | 9.36 |
As can be seen from the table, fertilizer provided by the invention can make the root of large-flowered skullcap plant volume increase effectively, wherein, embodiment 3 and reference examples 1 contrast, and can find out, are hydrolyzed to bacterium slag by introducing proteolytic enzyme, the single plant yield of the root of large-flowered skullcap can be improved, embodiment 3 and reference examples 2 contrast, and can find out to be hydrolyzed to bacterium slag by introducing cellulase, can improve the content of baicalin.
Claims (3)
1., for a preparation method for the bacterium residue organic fertilizer of root of large-flowered skullcap plantation, it is characterized in that, comprise the steps:
1st step, by weight, gets mushroom plantation bacterium slag 70 ~ 90 parts, is crushed to the powder of diameter 0.5 ~ 1mm; To add the water of 3 ~ 6 times of weight, then add 1 ~ 3 part, proteolytic enzyme and cellulase 1 ~ 3 part, heating, enzymolysis, then go out enzyme, lets cool, obtain enzymolysis solution;
2nd step, add in enzymolysis solution and dry cow dung 40 ~ 50 parts, maize straw 30 ~ 50 parts, cottonseed cake 20 ~ 30 parts, humic acid 5 ~ 10 parts, 10 ~ 20 parts, wheat bran, 10 ~ 20 parts, rice bran, dregs of beans 10 ~ 20 parts, 1 ~ 3 part, yeast, mix, ferment after re-cover film after accumulation, after fermentation ends, airing to moisture content is 20 ~ 30wt% again, obtains fermentation fertile;
3rd step, by fermentation fertilizer mix with 5 ~ 10 parts, diatomite, vermiculite 5 ~ 10 parts, wilkinite 10 ~ 16 parts, 10 ~ 20 parts, urea, monoammonium phosphate 6 ~ 8 parts, potassium sulfate 6 ~ 8 parts, wooden carbon dust 10 ~ 15 parts, calcium superphosphate 5 ~ 12 parts after; in the granulation of tablets press top die, obtain fertilizer.
2. the preparation method of the bacterium residue organic fertilizer for root of large-flowered skullcap plantation according to claim 1, it is characterized in that: Heating temperature is 40 ~ 50 DEG C, hydrolysis temperature is 3 ~ 6 hours.
3. the preparation method of the bacterium residue organic fertilizer for root of large-flowered skullcap plantation according to claim 1, is characterized in that: leavening temperature 30 ~ 40 DEG C, fermentation time 20 ~ 30 days.
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Cited By (16)
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| CN104803723A (en) * | 2015-05-10 | 2015-07-29 | 新疆桦骏昌科技有限公司 | Preparation process of ecological organic fertilizer |
| CN104892152A (en) * | 2015-05-27 | 2015-09-09 | 李孟平 | Preparation method for bio-fertilizer for scutellaria baicalensis planting |
| CN104926405A (en) * | 2015-06-11 | 2015-09-23 | 湖北诺维尔化肥有限公司 | Compound bioorganic fertilizer and preparation method thereof |
| CN105254354A (en) * | 2015-09-17 | 2016-01-20 | 山东佐田氏生物科技有限公司 | Preparation method for polymorphic bio-polypeptide organic fertilizer |
| CN105519794A (en) * | 2016-01-14 | 2016-04-27 | 孟红琳 | Preparation method of fermenting feed for culturing sea cucumber |
| CN105948892A (en) * | 2016-04-28 | 2016-09-21 | 孟红琳 | Method for preparing organic fertilizer coated with degradable material |
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| CN106578723A (en) * | 2016-12-17 | 2017-04-26 | 王先涛 | Letinous edodes residue fermented feed and breeding method for raising meat quality of genetically improved farmed tilapia |
| CN107721695A (en) * | 2017-12-08 | 2018-02-23 | 福清市冠盛蔬菜种植农民专业合作社 | A kind of organic fertilizer and its production method using bacteria residue production |
| CN108558499A (en) * | 2018-04-09 | 2018-09-21 | 山东省农业科学院农业资源与环境研究所 | A kind of compound formulation for melon crop of the extract containing edible fungi residue |
| CN108675849A (en) * | 2018-08-27 | 2018-10-19 | 河北省林业科学研究院 | A kind of middle micro element organic bio-fertilizer and preparation method thereof |
| CN109258365A (en) * | 2018-11-19 | 2019-01-25 | 济宁市食品药品检验检测中心（济宁市药品不良反应与药物滥用监测中心） | A kind of cultural method improving radix scutellariae root activity |
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| CN110981580A (en) * | 2019-12-25 | 2020-04-10 | 夏纪毅 | Preparation method of biological fertilizer for scutellaria baicalensis planting |
| CN113875472A (en) * | 2021-09-16 | 2022-01-04 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN115536477A (en) * | 2022-10-11 | 2022-12-30 | 承德医学院 | Organic fertilizer special for scutellaria baicalensis and application thereof |
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| CN104892152A (en) * | 2015-05-27 | 2015-09-09 | 李孟平 | Preparation method for bio-fertilizer for scutellaria baicalensis planting |
| CN104926405A (en) * | 2015-06-11 | 2015-09-23 | 湖北诺维尔化肥有限公司 | Compound bioorganic fertilizer and preparation method thereof |
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| CN109258365B (en) * | 2018-11-19 | 2021-03-26 | 济宁市食品药品检验检测中心（济宁市药品不良反应与药物滥用监测中心） | Cultivation method for improving root activity of scutellaria baicalensis |
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| CN109588216B (en) * | 2018-11-26 | 2024-03-15 | 樟树市仁德中药饮片有限公司 | Planting method suitable for various traditional Chinese medicines |
| CN110981580A (en) * | 2019-12-25 | 2020-04-10 | 夏纪毅 | Preparation method of biological fertilizer for scutellaria baicalensis planting |
| CN113875472A (en) * | 2021-09-16 | 2022-01-04 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN113875472B (en) * | 2021-09-16 | 2023-02-21 | 中南林业科技大学 | Tea oil tree planting method through soil improvement |
| CN115536477A (en) * | 2022-10-11 | 2022-12-30 | 承德医学院 | Organic fertilizer special for scutellaria baicalensis and application thereof |
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