CN103652364B - High-calcium leaf meal feed as well as production method and application thereof - Google Patents
High-calcium leaf meal feed as well as production method and application thereof Download PDFInfo
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Abstract
The invention relates to a high-calcium leaf meal feed as well as a production method and an application thereof. According to the high-calcium leaf meal feed, hybridized paper mulberry leaf meal is used as a main raw material and is matched with a proper amount of prunus humilis bunge leaf meal and silkworm sand; a microorganism cascade fermentation technology is used for producing a high-quality livestock and poultry feed; raw materials of the produced feed are composed of the paper mulberry leaf meal, the runus humilis bunge leaf meal and the silkworm sand in a ratio of 3:2:(1.5-1.8). The cascade fermentation technology is a three-grade differential fermentation method including high temperature fermentation, low temperature fermentation and middle temperature fermentation; the high temperature fermentation is to kill heterogeneous bacteria at 60-75 DEG C; the low temperature fermentation is to cultivate saccharomyces cerevisiae and aspergillus oryzae at 30-32 DEG C; the middle temperature fermentation is to cultivate lactobacillus plantarum at 35-40 DEG C; after the three grades of the differential fermentation including the high temperature fermentation, the low temperature fermentation and the middle temperature fermentation, the nutritional quality of the feed is greatly improved and the total amount of probiotics viable bacteria in the feed reaches 500,000-1,000,000cfu/g.
Description
Technical field
The present invention relates to a kind of high-calcium leaf meal feed and production method thereof and application, feed of the present invention is the high-quality antimicrobial-free feed that a kind of nutritional quality is very high, is widely used in organic raising of pig, chicken, duck, fish etc.Belong to feed processing technology field.
The present invention relates to a kind of high-calcium leaf meal feed and production method thereof and application, this feed is different from traditional paper mulberry leaf feed part and is: feedstuff is made up of feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand, and feed quality is high clean, high nutrition, high calcium nonreactive;
The production method of a kind of high-calcium leaf meal feed of the present invention is the microorganism cascade fermentation technology of segmentation difference fermentation;
The application of a kind of high-calcium leaf meal feed of the present invention, this feed can be widely used in organic raising of pig, chicken, duck, fish etc.
Background technology
In the document of prior art, feed with paper-mulberry leaf can directly as ensilage feeding animals, also can by feed with paper-mulberry leaf together with a small amount of stalk after fermentation process feeding animals, but the paper mulberry leaf feed of produced in conventional processes due to raw material ratio more single, sweat is gentleer, thus the nutritional quality of feed is limited, and cannot overcome growing of miscellaneous bacteria in feed, harmful levels of pathogens and parasitic ovum.
Feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand are carried out ternary complementary combinations by the present invention, and by the cascade fermentation technology that segmentation difference is fermented, fermentation process is carried out to compound leaf powder feed, make not only high clean, the high nutrition of produced feed, and high calcium nonreactive, thus overcome the defect of produced in conventional processes paper mulberry leaf feed.
The feed with paper-mulberry leaf powder used in the present invention, leaf of GAIGUO powder and silkworm sand are all the feed resources of high-quality cheapness, especially protein and organic calcium content very abundant, their nutritional quality is expressed as follows respectively:
(1) nutritional quality of feed with paper-mulberry leaf powder
Paper mulberry (Broussonetia papyrifera) is that Moraceae paper mulberry belongs to deciduous tree, its well developed root system, impoverishment tolerant, drought-resistant, Resistant etc., even if at arid, the barren place such as hills, hillside fields, rock seam, also have extremely strong vitality.
The nutritional labeling of feed with paper-mulberry leaf is very abundant, wild paper mulberry cured leaf amyloid proteins matter content reaches 26-32.54%, the cured leaf amyloid proteins matter content of hybridizing feeding paper mulberry (blade is without hair) also (sees the following form) between 20%-30%, on average about about 25%, its amino acid content be rice, 3 times of corn; The content of organic calcium also up to more than 3.4%, be soya bean, 5 times of milk; The content of vitamin, trace element be fruit, tens times of vegetables; The content of carrotene is also 3-5 times of carrot.Therefore, the feed with paper-mulberry leaf powder after feed with paper-mulberry leaf and roughing thereof is very good high-quality feed source.Hybridization feed with paper-mulberry leaf conventional nutrients is as shown in table 1 below.
Table 1 hybridizes feed with paper-mulberry leaf conventional nutrients (%)
| Crude protein | Crude fat | Crude fibre | Coarse ash | Calcium | Total phosphorus |
| 26.1 | 5.2 | 28.9 | 15.4 | 3.4 | 0.23 |
(2) nutritional quality of leaf of GAIGUO powder
Calcium fruit original name Europe Lee (Cerasus dictyoneura) is a kind of fruit shrubs of rose department cherry genus, Ye Shi China distinctive high calcium fruit seeds, and because of calcareous very capable of its enrichment from soil, fruit calcium content is very high, therefore named " calcium fruit ".Calcium fruit be mainly distributed in China three northern areas of China, happiness light, barren-resistant, drought-resistant, Salt And Alkali Tolerance, cold resistant, is suitable for growing in the soil of pH6.5-8.0, especially to drought condition and Karst lime soil adaptability very strong.
Calcium fruit plant is short and small, and grow thickly, plant height 0.5-1.5 rice, annual shoot canescence, young sprout celadon, sprig is by pubescence.After fruit maturation, likeness in form cherry, taste is like plum, and sour and sweet palatability, unique flavor, nutritious, wherein organic calcium, iron content occupy the hat of all kinds of fruit, and calcic 60-90 milligram in every 100 grams of fresh fruits, iron 1.5-2.5 milligram, is rich in vitamin A, Cobastab in addition
2, Cobastab
12, vitamin C, vitamin E and trace element manganese, zinc, magnesium, selenium etc., 17 seed amino acid content, up to 338.3-451.7mg/100g, are the high-class healthy fruit of children, old man and sub-health population.
Calcium stem end leaf nutritive value also very high (seeing the following form), not only to grow general nutriment, the especially important source of replenishing the calcium of cattle and sheep skeleton development such as required sugar, protein containing cattle and sheep.Therefore, the calcium stem end leaf powder after calcium stem end leaf and roughing thereof is also the important source material of producing animal and fowl fodder.Calcium stem end leaf conventional nutrients is as shown in table 2 below.
Table 2 calcium stem end leaf conventional nutrients (%)
| Crude protein | Crude fat | Crude fibre | Coarse ash | Calcium | Total phosphorus |
| 10.84 | 3.62 | 67.5 | 6.18 | 3.72 | 0.19 |
(3) nutritional quality of silkworm sand
Silkworm sand is the ight soil after nibbling mulberry leaf, more precisely, is the mixture of silkworm faeces, integument and mulberry leaf residue.Silkworm sand nutritious (seeing the following form), its nutritive value is similar to fine rice bran, wheat bran and secondary powder, is the perfect match feed of raising pig, chicken, duck, fish etc.
In fresh silkworm sand, moisture accounts for 55-65%, suitable with the soil humidity required for fermentable.In air-dry silkworm sand, organic matter accounts for 83.77-90.44%, and ash content accounts for 9.56-16.23%, and carbon-nitrogen ratio (C/N) is 31:1.In addition, also containing abundant bioactivator in silkworm sand, as pectin 10-20%, phytosterol 0.25-0.29%, chlorophyll 0.8%-1.0%, and vitamin E, K and carotenoid etc.The husky conventional nutrients of air-dry silkworm is as shown in table 3 below.
The husky conventional nutrients (%) of the air-dry silkworm of table 3
| Crude protein | Crude fat | Crude fibre | NFE | Calcium | Total phosphorus |
| 12.16 | 3.5 | 12.37 | 45.07 | 3.36 | 0.17 |
In view of feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand all have good nutritional quality, especially protein and organic calcium content very abundant, therefore, be necessary by these three kinds of high-quality feed material combination together, a kind of production method of high-calcium leaf meal feed is proposed, a kind of high-calcium leaf meal feed involved in the present invention that Here it is and production method thereof and application.
Summary of the invention
The object of this invention is to provide a kind of high-calcium leaf meal feed of high-quality, this feed is rich in protein, amino acid, vitamin and the trace element such as organic calcium, iron especially, pressed 10-30% consumption (mass percentage, if no special instructions, the all about consumption related in the application or concentration percentage composition all refer to mass percentage) coordinate with complete feed live pig of feeding, feedstuff-meat ratio can reach 2.6-2.8:1, daily gain 0.561-0.625kg.
Another object of the present invention is to provide a kind of production method of high-calcium leaf meal feed, and the method utilizes microorganism cascade fermentation technology to be produced into high-quality animal and fowl fodder, and described microorganism is S. cervisiae, aspergillus oryzae and Lactobacillus plantarum; Described cascade fermentation technology is high temperature, low temperature and middle temperature three grades of difference fermentation methods, hot fermentation goes out except miscellaneous bacteria at 60-75 DEG C, cold fermentation cultivates S. cervisiae and aspergillus oryzae at 30-32 DEG C, and mesophilic digestion is the lactobacillus that cultivates plants under 35-40 DEG C of condition.By this cascade fermentation technology, both effectively can remove miscellaneous bacteria, harmful levels of pathogens and the parasitic ovum in feed, nutritional quality and the probiotics viable bacteria quantity of feed can have been increased substantially again.
3rd object of the present invention is to provide a kind of application process of high-calcium leaf meal feed, namely to feed live pig with this feed, breeding cycle can be shortened, improve efficiency of feed utilization, also contribute to improving animal intestinal micro-ecology environment simultaneously, strengthen animal immunizing power and premunition, thus can antibiotic dosage be reduced, improve and raise quality.
Particularly, the object of the present invention is achieved like this:
First, provide a kind of production method of above-mentioned high-calcium leaf meal feed, described method comprises the steps: in the present invention
(1) fermentation material is formed by feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand by the proportioning of 3:2:1.5-2.5, stir and evenly mix, and add clear water and make the humidity of fermentation material remain on 60-65%, then complete cascade fermentation process by following (2), (3), (4) step successively;
(2) the hot fermentation stage: add feed enzyme preparation by 2-5% consumption in the fermentation material that described step (1) obtains, mix with above-mentioned fermentation material, load indoor ferment bed, filling consolidation, cover ferment bed with plastic face mask, so that enzyme preparation and natural miscellaneous bacteria can Rapid Fermentations, treat that ferment bed tempertaure rises to more than 60 DEG C, continue 2-3 days, the fermentation material substantially become thoroughly decomposed;
(3) the cold fermentation stage: after the fermentation material substantially become thoroughly decomposed obtained in above-mentioned steps (2) is chilled to room temperature, then S. cervisiae and aspergillus oryzae bacterial classification are evenly inoculated in fermentation material by 2-5% consumption, two kinds of bacterial classification consumptions are identical, ferment bed humidity remains on 60-65%, temperature controls at 30-32 DEG C, continue overlay film fermentation, summer 2-3 days, winter 3-5 days;
(4) the mesophilic digestion stage: after treating that above-mentioned steps (3) process terminates, again Lactobacillus plantarum bacterial classification is evenly inoculated in fermentation material by 2-5% consumption, ferment bed humidity remains on 60-65%, temperature controls at 35-40 DEG C, because Lactobacillus plantarum belongs to anaerobism or facultative anaerobic bacteria, continues overlay film fermentation, summer 2-3 days, winter, 3-5 days, after having fermented, obtained described high-quality high-calcium leaf meal feed.
Wherein, state in the production method of high-calcium leaf meal feed on the invention, in step (2), fermentation material becomes thoroughly decomposed substantially, miscellaneous bacteria, harmful levels of pathogens and various parasitic ovum are gone out and are removed, heavy large biological molecule is also by Partial digestion, and small molecule bioactive amount of material increases, and nutritional quality improves greatly;
In step (3), after having fermented, the zymohydrolysis of various enzyme secreted by S. cervisiae and aspergillus oryzae, the savory spilling of fermentation material, its nutritional quality improves further;
The inventive method gained feed is can be very high and be rich in the high-quality high-calcium leaf meal feed of beneficial flora for the feeding nutritional quality of livestock and poultry, and in feed, probiotics viable bacteria total amount can reach 50-100 ten thousand cfu/g.
Wherein, state on the invention in the production method of high-calcium leaf meal feed:
Feed enzyme preparation described in step (2) is the complex enzyme formulation deriving from food industry microorganism aspergillus oryzae or derive from food industry microorganism black-koji mould, main containing amylase, lipase, protease, phytase and zytase etc. in this feed enzyme preparation, this feed enzyme preparation is the material that those skilled in the art commonly use, commercially can buy and obtain, also can directly replace this feed enzyme preparation to use with aspergillus oryzae powder or black-koji mould powder, its effect be the quick startup promoting hot fermentation;
S. cervisiae described in step (3) is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 28-30 DEG C;
Described aspergillus oryzae is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 26-32 DEG C.
Lactobacillus plantarum described in step (4) is cultivated in the following manner:
A, slant medium: potato 10%, banana 5%, soy meal 5%, white sugar 2%, K
2hPO
44%, calcium carbonate 1%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 30%, rice bran 30%, bean cake powder 40%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 35-40 DEG C, initial ph value 6.8-7.2, last pH value 6.0-6.5.
Secondly, provide a kind of high-calcium leaf meal feed, this feed is obtained by the production method of the above-mentioned high-calcium leaf meal feed of the present invention in the present invention.
The high-calcium leaf meal feed of the present invention obtained thus, its carbon-nitrogen ratio (C/N) is 25-30:1, crude protein content is 16.5-21.3%, crude fat content is 3.05-3.38%, carbohydrate content is 45.23-50.47%, 18 seed amino acid total contents are 9.35-10.28%, and organic calcium content is 3.36-3.72%, in addition containing bioactivator and various trace elements such as multivitamin, carotenoid, phytosterol, flavonoids;
Further, the LD of described feed
50>12g/kg, the nontoxic level feed in true border; Probiotics viable bacteria total amount in described feed can reach 50-100 ten thousand cfu/g.
The present invention is last, also provides the purposes of a kind of above-mentioned high-calcium leaf meal feed in live pig of feeding.
High-calcium leaf meal feed of the present invention is different from other biological fermentation feed part and is:
(1) first belong to clean feeds, by the hot fermentation stage in cascade fermentation technology, effectively remove the miscellaneous bacteria in feed, harmful levels of pathogens and parasitic ovum;
(2) secondly belong to high calcium feed, by the ternary complementary combinations of feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand, greatly enrich the content of the mineral elements such as the organic calcium in feed, iron;
(3) high-quality non-antibiotic feed is belonged to, by three grades of difference sweats, in feed, heavy large biological molecule is fully degraded, the bioactivators such as the protein in feed, amino acid, vitamin, carotenoid, flavonod are increased greatly, also be rich in a large amount of beneficial flora and secreted biology enzyme system thereof that comprise Lactobacillus plantarum in feed simultaneously, in detoxification process, because immunity and premunition improve greatly, thus antibiotic a large amount of use can be exempted.
The present invention hybridizes feed with paper-mulberry leaf powder for primary raw material, and coordinates with appropriate leaf of GAIGUO powder and silkworm sand, and utilize the high-quality high-calcium leaf meal feed that microorganism cascade fermentation technology is produced, the nutritional quality of this feed is mainly manifested in:
(1) be rich in the biology enzyme system contributing to nutrient digestion absorption of the beneficial floras such as S. cervisiae, aspergillus oryzae and Lactobacillus plantarum and secretion thereof, can improve by the immunity of feeding animals and premunition, thus avoid a large amount of antibiotic use;
(2) by high temperature, low temperature, the three grades of difference fermentations of middle temperature, both gone out except miscellaneous bacteria, harmful levels of pathogens, parasitic ovum, make again heavy large biological molecule fully be degraded, greatly enrich the content of the bioactivators such as the organic acid in feed, vitamin, biotin;
(3) due to the ternary complementary combinations of feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand, make in the feed after undergoing microbial fermentation, to be enriched a large amount of organic calciums, iron, phosphorus, potassium and other trace element.
Special innovation of the present invention be in: Modern microbiological fermented feed produce in, the ingenious cascade fermentation technology applying the fermentation of segmentation difference, both the miscellaneous bacteria in feed, harmful levels of pathogens and parasitic ovum had been killed, substantially increase again beneficial flora and the nutritional quality of feed, make not only to be grown quickening by the animal such as pig, chicken, duck, fish fed, shorten breeding cycle, improve efficiency of feed utilization, improve the microecosystem of animal intestinal simultaneously, enhance immunity and the premunition of animal, decrease antibiotic dosage, improve raising quality.
Detailed description of the invention
Embodiment given is below for further illustrating technical characteristic of the present invention, not limits technical scheme of the present invention.To any modification or partial replacement that the technical scheme in the present invention is carried out, without departing from the spirit and scope of the present invention, all should be encompassed in protection scope of the present invention.
Embodiment 1
(1) form fermentation material by feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand by the proportioning of 3:2:1.5, stir and evenly mix, and add clear water and make the humidity of fermentation material remain on 60-65%, then complete cascade fermentation process by following (2), (3), (4) step successively:
(2) the hot fermentation stage: add feed enzyme preparation by 2% consumption in the fermentation material that described step (1) obtains, mix with above-mentioned fermentation material, load indoor ferment bed, filling consolidation, cover ferment bed with plastic face mask, so that enzyme preparation and natural miscellaneous bacteria can Rapid Fermentations, treat that ferment bed tempertaure rises to more than 60 DEG C, continue 2-3 days, the fermentation material substantially become thoroughly decomposed;
(3) the cold fermentation stage: after the fermentation material substantially become thoroughly decomposed obtained in above-mentioned steps (2) is chilled to room temperature, then S. cervisiae and aspergillus oryzae bacterial classification are evenly inoculated in fermentation material by 2% consumption, two kinds of bacterial classification consumptions are identical, ferment bed humidity remains on 60-65%, temperature controls at 30-32 DEG C, continue overlay film fermentation, summer 2-3 days, winter 3-5 days;
(4) the mesophilic digestion stage: after treating that above-mentioned steps (3) process terminates, again Lactobacillus plantarum bacterial classification is evenly inoculated in fermentation material by 2% consumption, ferment bed humidity remains on 60-65%, temperature controls at 35-40 DEG C, because Lactobacillus plantarum belongs to anaerobism or facultative anaerobic bacteria, continues overlay film fermentation, summer 2-3 days, winter, 3-5 days, after having fermented, obtained described high-quality high-calcium leaf meal feed.
Wherein said feed enzyme preparation is described by summary of the invention part; The mode that S. cervisiae, aspergillus oryzae and Lactobacillus plantarum describe by summary of the invention part is cultivated.
Record with the standard method of analysis of organic compound each in elemental analyser and biological organic matter, the carbon-nitrogen ratio (C/N) that this embodiment produces the high-calcium leaf meal feed obtained is 28-30:1, crude protein content is 16.5-18.6%, crude fat content is 3.05-3.13%, carbohydrate content is 48.56-50.47%, 18 seed amino acid total contents are 9.35-9.67%, organic calcium content is 3.36-3.42%, in addition containing bioactivator and various trace elements such as multivitamin, carotenoid, phytosterol, flavonoids; The LD of described feed
50>12g/kg, the probiotics viable bacteria total amount simultaneously in feed can reach 50-60 ten thousand cfu/g.
Embodiment 2
(1) form fermentation material by feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand by the proportioning of 3:2:2, stir and evenly mix, and add clear water and make the humidity of fermentation material remain on 60-65%, then complete cascade fermentation process by following (2), (3), (4) step successively:
(2) the hot fermentation stage: add feed enzyme preparation by 3% consumption in the fermentation material that described step (1) obtains, mix with above-mentioned fermentation material, load indoor ferment bed, filling consolidation, cover ferment bed with plastic face mask, so that enzyme preparation and natural miscellaneous bacteria can Rapid Fermentations, treat that ferment bed tempertaure rises to more than 60 DEG C, continue 2-3 days, the fermentation material substantially become thoroughly decomposed;
(3) the cold fermentation stage: after the fermentation material substantially become thoroughly decomposed obtained in above-mentioned steps (2) is chilled to room temperature, then S. cervisiae and aspergillus oryzae bacterial classification are evenly inoculated in fermentation material by 3% consumption, two kinds of bacterial classification consumptions are identical, ferment bed humidity remains on 60-65%, temperature controls at 30-32 DEG C, continue overlay film fermentation, summer 2-3 days, winter 3-5 days;
(4) the mesophilic digestion stage: after treating that above-mentioned steps (3) process terminates, again Lactobacillus plantarum bacterial classification is evenly inoculated in fermentation material by 3% consumption, ferment bed humidity remains on 60-65%, temperature controls at 35-40 DEG C, because Lactobacillus plantarum belongs to anaerobism or facultative anaerobic bacteria, continues overlay film fermentation, summer 2-3 days, winter, 3-5 days, after having fermented, obtained described high-quality high-calcium leaf meal feed.
Wherein said feed enzyme preparation is described by summary of the invention part; The mode that described S. cervisiae, aspergillus oryzae and Lactobacillus plantarum describe by summary of the invention part is cultivated.
Record with the standard method of analysis of organic compound each in elemental analyser and biological organic matter, the carbon-nitrogen ratio (C/N) that this embodiment produces the high-calcium leaf meal feed obtained is 26-28:1, crude protein content is 17.3-18.5%, crude fat content is 3.15-3.21%, carbohydrate content is 47.02-48.31%, 18 seed amino acid total contents are 9.60-9.85%, organic calcium content is 3.42-3.64%, in addition containing bioactivator and various trace elements such as multivitamin, carotenoid, phytosterol, flavonoids; The LD of described feed
50>12g/kg, the probiotics viable bacteria total amount simultaneously in feed can reach 60-80 ten thousand cfu/g.
Embodiment 3
(1) form fermentation material by feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm sand by the proportioning of 3:2:2.5, stir and evenly mix, and add clear water and make the humidity of fermentation material remain on 60-65%, then complete cascade fermentation process by following (2), (3), (4) step successively:
(2) the hot fermentation stage: add feed enzyme preparation by 5% consumption in the fermentation material that described step (1) obtains, mix with above-mentioned fermentation material, load indoor ferment bed, filling consolidation, cover ferment bed with plastic face mask, so that enzyme preparation and natural miscellaneous bacteria can Rapid Fermentations, treat that ferment bed tempertaure rises to more than 60 DEG C, continue 2-3 days, the fermentation material substantially become thoroughly decomposed;
(3) the cold fermentation stage: after the fermentation material substantially become thoroughly decomposed obtained in above-mentioned steps (2) is chilled to room temperature, then S. cervisiae and aspergillus oryzae bacterial classification are evenly inoculated in fermentation material by 5% consumption, two kinds of bacterial classification consumptions are identical, ferment bed humidity remains on 60-65%, temperature controls at 30-32 DEG C, continue overlay film fermentation, summer 2-3 days, winter 3-5 days;
(4) the mesophilic digestion stage: after treating that above-mentioned steps (3) process terminates, again Lactobacillus plantarum bacterial classification is evenly inoculated in fermentation material by 5% consumption, ferment bed humidity remains on 60-65%, temperature controls at 35-40 DEG C, because Lactobacillus plantarum belongs to anaerobism or facultative anaerobic bacteria, continues overlay film fermentation, summer 2-3 days, winter, 3-5 days, after having fermented, obtained described high-quality high-calcium leaf meal feed.
Wherein said feed enzyme preparation is described by summary of the invention part; The mode that described S. cervisiae, aspergillus oryzae and Lactobacillus plantarum describe by summary of the invention part is cultivated.
Record with the standard method of analysis of organic compound each in elemental analyser and biological organic matter, the carbon-nitrogen ratio (C/N) that this embodiment produces the high-calcium leaf meal feed obtained is 25-26:1, crude protein content is 19.4-21.3%, crude fat content is 3.27-3.38%, carbohydrate content is 45.23-46.85%, 18 seed amino acid total contents are 9.87-10.28%, organic calcium content is 3.69-3.72%, in addition containing bioactivator and various trace elements such as multivitamin, carotenoid, phytosterol, flavonoids; The LD of described feed
50>12g/kg, the probiotics viable bacteria total amount simultaneously in feed can reach 90-100 ten thousand cfu/g.
Embodiment 4
An application for high-calcium leaf meal feed, namely to feed live pig with this feed, specific practice is:
Method one: the feed described in embodiment 1-3 adding 10-15% in pig complete feed respectively, can promote the appetite of pig, makes bristles color light, quickening of growing, improves and fatten rate 12.3-15.5%;
Method two: feed described in the embodiment 1-3 adding 15-30% in pig complete feed, not only the hair color light of live pig, quickenings of growing, and also immune disease-resistance power improves 30-50%, and daily gain improves 6.07-9.15%, feedstuff-meat ratio reduction 5.09-8.38%;
Method three: if need the ratio of feeding increasing feed described in embodiment 1-3 further, its consumption in pig complete feed is made to reach more than 30%, can by observing the reaction of feeding of pig, with degree of being adapted to, slowly increase consumption, also need supplementary suitable nutrition simultaneously, such as: when described feed consumption reaches 60%, also need to add wheat bran (or rice bran) 13%, corn flour (or sweet potato powder) 23% and premix 4%, can certainly the premix of 4% type, because the vitamin in described feed and organic calcium, the micronutrient levels such as iron are very abundant, need not add in addition.
In the present embodiment, the feed of the present invention of use belongs to the quality product feed containing high protein, high calcium, high beneficial flora, should with system with or with system, the feed produced preferably was finished in 24-48 hour, if can not be finished for the moment, seal up for safekeeping in time, do not leak gas, in order to avoid putrid and deteriorated.
Claims (11)
1. a production method for high-calcium leaf meal feed, is characterized in that, described method comprises the steps:
(1) fermentation material is formed by the husky proportioning by 3: 2: 1.5-2.5 of feed with paper-mulberry leaf powder, leaf of GAIGUO powder and silkworm, stir and evenly mix, and add clear water and make the humidity of fermentation material remain on 60-65%, then complete cascade fermentation process by following (2), (3), (4) step successively;
(2) the hot fermentation stage: add feed enzyme preparation by 2-5% consumption in the fermentation material that described step (1) obtains, mix with above-mentioned fermentation material, load indoor ferment bed, filling consolidation, cover ferment bed with plastic face mask, so that enzyme preparation and natural miscellaneous bacteria can Rapid Fermentations, treat that ferment bed tempertaure rises to more than 60 DEG C, continue 2-3 days, obtain the fermentation material become thoroughly decomposed;
(3) the cold fermentation stage: after the fermentation material become thoroughly decomposed obtained in above-mentioned steps (2) is chilled to room temperature, then S. cervisiae and aspergillus oryzae bacterial classification are evenly inoculated in fermentation material by 2-5% consumption, two kinds of bacterial classification consumptions are identical, ferment bed humidity remains on 60-65%, temperature controls at 30-32 DEG C, continue overlay film fermentation, summer 2-3 days, winter 3-5 days;
(4) the mesophilic digestion stage: after treating that above-mentioned steps (3) process terminates, again Lactobacillus plantarum bacterial classification is evenly inoculated in fermentation material by 2-5% consumption, ferment bed humidity remains on 60-65%, temperature controls at 35-40 DEG C, because Lactobacillus plantarum belongs to anaerobism or facultative anaerobic bacteria, continues overlay film fermentation, summer 2-3 days, winter, 3-5 days, after having fermented, obtained described high-quality high-calcium leaf meal feed.
2. the production method of high-calcium leaf meal feed according to claim 1, it is characterized in that, the feed enzyme preparation described in step (2) is the complex enzyme formulation deriving from food industry microorganism aspergillus oryzae or derive from food industry microorganism black-koji mould.
3. the production method of high-calcium leaf meal feed according to claim 1 and 2, is characterized in that, main containing amylase, lipase, protease, phytase and zytase in described feed enzyme preparation.
4. the production method of the high-calcium leaf meal feed according to any one of claim 1-2, is characterized in that, the S. cervisiae described in step (3) is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 28-30 DEG C.
5. according to the production method of the high-calcium leaf meal feed described in claim 3, it is characterized in that, the S. cervisiae described in step (3) is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 28-30 DEG C.
6. the production method of the high-calcium leaf meal feed according to any one of claim 1-2, is characterized in that, the aspergillus oryzae described in step (3) is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 26-32 DEG C.
7. according to the production method of the high-calcium leaf meal feed described in claim 3, it is characterized in that, the aspergillus oryzae described in step (3) is cultivated in the following manner:
A, slant medium: potato 20%, banana 5%, white sugar 2%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 50%, rice bran 40%, bean cake powder 10%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 26-32 DEG C.
8. the production method of the high-calcium leaf meal feed according to any one of claim 1-2,5,7, is characterized in that, the Lactobacillus plantarum described in step (4) is cultivated in the following manner:
A, slant medium: potato 10%, banana 5%, soy meal 5%, white sugar 2%, K
2hPO
44%, calcium carbonate 1%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 30%, rice bran 30%, bean cake powder 40%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 35-40 DEG C, initial ph value 6.8-7.2, last pH value 6.0-6.5.
9. according to the production method of the high-calcium leaf meal feed described in claim 3, it is characterized in that, the Lactobacillus plantarum described in step (4) is cultivated in the following manner:
A, slant medium: potato 10%, banana 5%, soy meal 5%, white sugar 2%, K
2hPO
44%, calcium carbonate 1%, coconut palm breast 1%, agar 2%, water 70%;
B, solid fermentation culture medium: wheat bran 30%, rice bran 30%, bean cake powder 40%, separately adds water appropriate, pinches agglomerating for degree with hand;
C, condition of culture: cultivation temperature 35-40 DEG C, initial ph value 6.8-7.2, last pH value 6.0-6.5.
10. a high-calcium leaf meal feed, is characterized in that this feed is obtained by the production method of the high-calcium leaf meal feed described in any one in claim 1-9.
The purposes of 11. high-calcium leaf meal feeds according to claim 10 in live pig of feeding.
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| CN103918885B (en) * | 2014-04-09 | 2015-12-30 | 厦门百穗行科技股份有限公司 | A kind of method utilizing feed with paper-mulberry leaf, balloonflower root, waste beer yeast slurry to prepare high protein feed |
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| CN107691849B (en) * | 2017-11-13 | 2021-06-29 | 播恩集团股份有限公司 | Feed prepared by microbial fermentation |
| CN108208401A (en) * | 2018-01-31 | 2018-06-29 | 周明钟 | One boar paper mulberry fluid biological feed and its processing technology |
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| CN109463565A (en) * | 2018-12-10 | 2019-03-15 | 云南农业大学 | A kind of feed with paper-mulberry leaf powder is the laying duck concentrate feed of part protein sources |
| KR102393124B1 (en) * | 2021-01-13 | 2022-05-03 | 농업회사법인 (주)한국유용곤충연구소 | Method for preparing fermented insect feed additive comprising Ptecticus tenebrifer and insect chitin, and fermented insect feed additive prepared by the same |
| CN113367233A (en) * | 2021-06-17 | 2021-09-10 | 梁吉汉 | Broussonetia papyrifera ecological feed and production method thereof |
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| CN1190144C (en) * | 2002-03-11 | 2005-02-23 | 刘尚文 | Paper mulberry leaf feed and its production method |
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