CN102774955A - Biological purification method of landscape water - Google Patents
Biological purification method of landscape water Download PDFInfo
- Publication number
- CN102774955A CN102774955A CN2012102623506A CN201210262350A CN102774955A CN 102774955 A CN102774955 A CN 102774955A CN 2012102623506 A CN2012102623506 A CN 2012102623506A CN 201210262350 A CN201210262350 A CN 201210262350A CN 102774955 A CN102774955 A CN 102774955A
- Authority
- CN
- China
- Prior art keywords
- landscape water
- water body
- landscape
- water
- complex microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a biological purification method of landscape water. According to the method, a microbial flocculant, a compound enzyme preparation and a compound microorganism preparation which are evenly mixed are added into the landscape water, the microbial flocculant is used for flocculating suspended solids in the landscape water, the compound enzyme preparation is used for enzymolysis of water nutrients in the landscape water, the compound microorganism preparation is used for degrading small molecule nutrients in the landscape water, suspended solids, ammonia nitrogen and total phosphorus in the landscape water are degraded to achieve the purification treatment of the landscape water. Three biological preparations are combined, a synergy function is provided, a multi-level effect is formed, water quality is improved remarkably, and the method has no toxic and side effects on landscape fish. The biological purification method is simple to operate, low in cost and remarkable in effect, and has obvious economical and social benefits.
Description
Technical field
The present invention relates to a kind of water body purification method, especially a kind of biotechnological formulation that adopts carries out the method that landscape water body purifies.
Background technology
As far as city appearance water body; Like park, colleges and universities, enterprises and institutions, medium-to-high grade sub-district, though outstanding planning and design are arranged, owing to lack specialized management, suitable water body purification method; Make many landscape water bodies or be in dry state, or water quality is not fully up to expectations; Particularly to summer, green, water quality fouling that the water colour of landscape water body turns has greatly influenced surrounding environment.
The water conditioning of landscape water body and water body safeguard it is puzzlement people technical barriers for many years always.Past people adopts chemical agent to carry out the water body reparation usually, but chemical agent tends to influence water ecology, increases other pollution loads of water body, and time consumption and energy consumption, the weak point of holding time.Therefore, along with the fast development of biotechnology, people have arrived biotechnology applications the purification of landscape water body; But; Because the landscape water pollution thing is complicated various, adopts single biopurification preparation often to cause scrubbing speed slow, the defective that the scrubbing effect is relatively poor.
Summary of the invention
The present invention is directed to the deficiency of prior art, a kind of method of biopurification landscape water is provided, it adopts three kinds of biotechnological formulation combined utilization, has the synergy function, forms multistage effect, and the water correction effect is remarkable, and the view fish is not had any malicious negatively influencing.Method of the present invention is simple to operate, and cost is low, and effect is remarkable, has tangible economic benefit and social benefit.
For realizing above technical purpose, the present invention will take following technical scheme:
A kind of method of biopurification landscape water; The microbial flocculant that mixes, compound enzymic preparation and complex microorganism preparations are dropped in the landscape water body; Utilize the small molecules nutritive substance in water body suspended substance in the microbial flocculant flocculation landscape water body, the water nutrition material in the compound enzymic preparation enzymolysis landscape water body, the complex microorganism preparations degraded landscape water body; Water body suspended substance, ammonia nitrogen and total phosphorus in the landscape water body are degraded, and realize the purifying treatment of landscape water body.
In every cubical landscape water body, the usage quantity of microbial flocculant, compound enzymic preparation and complex microorganism preparations is followed successively by: 5-8g, 3-5g, 3-5g.
Said microbial flocculant is prepared from such a way: in the fermentation tank culture medium after sterilising treatment; Inoculum size inoculation colloid bacillus cereus with 8%; After 30 ℃ of following aerobics are cultivated 20～24 hours, add the absolute ethyl alcohol of fermented liquid TV 30%, to separate out polysaccharose substance; Then the mass ratio according to 1～4:1 adds wheat bran in polysaccharose substance, and then the mixture fluidized drying in fluidized-bed with polysaccharose substance and wheat bran gets final product; The culture medium prescription of colloid bacillus cereus is: sucrose 1.5%, and peptone 1.0%, yeast extract paste 0.5%, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01%, potassium primary phosphate 0.15%, sodium-chlor 1%, surplus is a water; Each components contents is mass fraction in this culture medium prescription; The containing quantity of substratum is 70% of a volume in the fermentor tank.
Said complex microorganism preparations is prepared from such a way: under 33 ℃, be inoculated into subtilis, Rhodopseudomonas palustris, candida tropicalis in the fermentor tank that is loaded with corresponding substratum; After 35 ℃ of temperature are cultivated 20～24 hours down; The spinning nutrient solution is then collected thalline, then in thalline, adds wheat bran with 1～4 times quality multiple; Mixture fluidized drying in fluidized-bed with thalline and wheat bran obtains the complex microorganism olry bacterial powder; The culture medium prescription of this complex microorganism preparations is: dregs of beans 1.6%, glucose 0.7%, starch 0.2%, yeast powder 0.05%; Potassium hydrogenphosphate 0.01%, potassium primary phosphate 0.02%, manganous sulfate 0.01%, sal epsom 0.05%; Lime carbonate 0.03%, sodium-chlor 0.05%, surplus is a water; Each components contents is mass fraction in this culture medium prescription; The containing quantity of substratum is 70% of a volume in the fermentor tank.
Said compound enzymic preparation comprises neutral protease, lypase and cellulase, and neutral protease, lypase and cellulase are composite according to the mass ratio of 1～3:1:1～2.
Neutral protease is the AS1.398 neutral protease of 30000 U/g for enzyme work; Lypase is the LBK-B400 lypase of 50000 U/g for enzyme work; The enzyme work of cellulase is 20000 U/mL.
According to above technical scheme, can realize following beneficial effect:
The present invention at first adopts biological flocculant can reduce the water body ss suspended solid content in the aquaculture water, improves water transparency; Utilizing the effect of compound enzymic preparation then, is small molecules with the remaining nutrition enzymolysis that flocculates to the bottom; Utilize the ammonification of complex microorganism preparations again; The medium and small molecular nutrition thing of water is resolved into ammonia nitrogen; Ammonia nitrogen resolves into nitroso-group nitrogen under the effect of nitrite bacteria, the nitrification through nitrobacteria becomes nitrate again, and nitrate can be through the effect of denitrifying bacterium; Become nitrogen and be discharged in the air and go, thereby play fast, thoroughly SS, ammonia nitrogen and the total phosphorus in the landscape water body supported in degraded.Three kinds of technical tie-ups are used has the synergy function, forms multistage effect and thoroughly improves water quality, and the view fish is not had any malicious negatively influencing.Method of the present invention is simple to operate, and cost is low, and effect is remarkable, has tangible economic benefit and social benefit.
Embodiment
Below will combine each embodiment that technical scheme of the present invention at length is described.But protection scope of the present invention can not be thought and only is confined to following specific embodiment.For said those skilled in the art, under the basic premise that does not break away from the present invention's design, can also make some simple deductions or be equal to replacement, these are equal to the row that alternative still will be regarded as protection scope of the present invention.
The method of biopurification landscape water according to the invention; The microbial flocculant that mixes, compound enzymic preparation and complex microorganism preparations are dropped in the landscape water body; Utilize the small molecules nutritive substance in water body suspended substance in the microbial flocculant flocculation landscape water body, the water nutrition material in the compound enzymic preparation enzymolysis landscape water body, the complex microorganism preparations degraded landscape water body; During small molecules nutritive substance in this complex microorganism degraded landscape water body, utilize the ammonification of complex microorganism preparations earlier, the medium and small molecular nutrition thing of water is resolved into ammonia nitrogen; Then ammonia nitrogen resolves into nitroso-group nitrogen under the effect of nitrite bacteria; Nitrification through nitrobacteria becomes nitrate again, and nitrate can be through the effect of denitrifying bacterium, becomes nitrogen and is discharged in the air and goes; Support SS, ammonia nitrogen and the total phosphorus in the landscape water body thereby playing fast, thoroughly degrades, realize the purifying treatment of landscape water body.
1, the preparation of microbial flocculant:
The employing massfraction is: sucrose 1.5%, and peptone 1.0%, yeast extract paste 0.5%, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01%, potassium primary phosphate 0.15%, sodium-chlor 1% joins substratum in the fermentor tank, and liquid volume added is 70% of a fermentor tank volume; Autoclaving 30 minutes, be cooled to 30 ℃ after the inoculation; The selection colloid bacillus cereus (
Bacillus mucilaginosus Krassilnikov), inoculum size is cultivated 20～24 hours (aerating oxygen) down for 8%, 30 ℃.The absolute ethyl alcohol that adds fermented liquid TV 30%; Separate out polysaccharose substance; In the polysaccharose substance of being separated out, add a certain amount of wheat bran; Its addition is 1～4 times of polysaccharose substance quality, and the mixture fluidized drying in fluidized-bed with above-mentioned polysaccharose substance and wheat bran obtains microbial flocculant.
2, the preparation of complex microorganism and prozyme solid preparation:
Take by weighing dregs of beans 1.6% glucose 0.7% by weight percentage, starch 0.2%, yeast powder 0.05%, potassium hydrogenphosphate 0.01%, potassium primary phosphate 0.02%, manganous sulfate 0.01%, sal epsom 0.05%, lime carbonate 0.03%, sodium-chlor 0.05%.With substratum with water dissolution after, join in the fermentor tank, liquid volume added is the 70%:121 ℃ of autoclaving 30 minutes of fermentor tank, be cooled to 33 ℃ after inoculation, insert subtilis (
Bacillus subtilis) (35 ℃), Rhodopseudomonas palustris (
Rhodopseudomonas palustris) (35 ℃), candida tropicalis (
Candida tropicalis) (35 ℃) three kinds of bacterial classifications, cultivated 20～24 hours, medium centrifugal is separated for 35 ℃; Collect thalline, in thalline, add the wheat bran of a certain amount of (thalline quality 1～4 times), mixture fluidized drying in fluidized-bed of thalline and wheat bran; Obtain the complex microorganism olry bacterial powder; Ratio in olry bacterial powder quality 30% adds proteolytic enzyme, lypase, glycase again, and wherein the ratio of three kinds of enzymes is 3:1:2, makes complex microorganism and prozyme solid preparation.Wherein: proteolytic enzyme is AS1.398 neutral protease (enzyme 30000 U/g alive; Available from the new glad Xiang space biotechnology in Xingtai ltd); Lypase is LBK-B400 lypase (enzyme 50000 U/g alive; Available from the Shenzhen Leveking Biology Engineering Co.,Ltd, cellulase (enzyme 20000 U/mL that live are available from outstanding promise enzyme ltd).
3, the method for use of preparation
The usage quantity of microbial flocculant, complex microorganism and prozyme solid preparation confirms that according to the pollution level of landscape water body in general, in every cube of landscape water body, its usage quantity is followed successively by 5-8g, 3-5g.
Landscape water is carried out in Zhejiang Hangzhou Lv Cheng sub-district.With the usage quantity of microbial flocculant by every cube 6 gram, complex microorganism and prozyme solid preparation are pressed every cube 4 usage quantity that restrains, add the water of ten times of weight, mix, evenly splash in full pond, monitoring water body each item index after seven days.
Each item water-quality guideline such as table 1 before water body is handled:
Table 1
| Index | BOD5 | CODCr | SS | NH3-N | TP |
| Before handling (mg/L) | 154 | 513 | 198 | 34 | 3.2 |
Handle back seven days each item water-quality guideline such as table 2 through biological flocculant and complex micro organism fungicide and compound enzymic preparation:
Table 2
| Index | BOD5 | CODCr | SS | NH3-N | TP |
| Handle back (mg/L) | 9.5 | 36 | 5.4 | 4.2 | 0.64 |
In table 1 and the table 2, BOD5:5 day biochemical oxygen demand; CODCr: the COD that potassium dichromate process is measured; SS: water quality suspended substance; NH3-N: ammonia-nitrogen content; TP: total phosphorous.
Claims (6)
1. the method for a biopurification landscape water; It is characterized in that; The microbial flocculant that mixes, compound enzymic preparation and complex microorganism preparations are dropped in the landscape water body; Utilize the small molecules nutritive substance in water body suspended substance in the microbial flocculant flocculation landscape water body, the water nutrition material in the compound enzymic preparation enzymolysis landscape water body, the complex microorganism preparations degraded landscape water body; Water body suspended substance, ammonia nitrogen and total phosphorus in the landscape water body are degraded, and realize the purifying treatment of landscape water body.
2. according to the method for the said biopurification landscape water of claim 1, it is characterized in that in every cubical landscape water body, the usage quantity of microbial flocculant, compound enzymic preparation and complex microorganism preparations is followed successively by: 5-8g, 3-5g, 3-5g.
3. according to the method for the said biopurification landscape water of claim 1; It is characterized in that; Said microbial flocculant is prepared from such a way: in the fermentation tank culture medium after sterilising treatment, the inoculum size inoculation colloid bacillus cereus with 8% is after 30 ℃ of following aerobics are cultivated 20～24 hours; The absolute ethyl alcohol that adds fermented liquid TV 30%; To separate out polysaccharose substance, then the mass ratio according to 1～4:1 adds wheat bran in polysaccharose substance, and then the mixture fluidized drying in fluidized-bed with polysaccharose substance and wheat bran gets final product; The culture medium prescription of colloid bacillus cereus is: sucrose 1.5%, and peptone 1.0%, yeast extract paste 0.5%, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.01%, potassium primary phosphate 0.15%, sodium-chlor 1%, surplus is a water; Each components contents is mass fraction in this culture medium prescription; The containing quantity of substratum is 70% of a volume in the fermentor tank.
4. according to the method for the said biopurification landscape water of claim 1; It is characterized in that; Said complex microorganism preparations is prepared from such a way: subtilis, Rhodopseudomonas palustris, candida tropicalis is inoculated under 33 ℃ in the fermentor tank that is loaded with corresponding substratum, and after 35 ℃ of temperature are cultivated 20～24 hours down, the spinning nutrient solution; Then collect thalline; Then in thalline, add wheat bran with 1～4 times quality multiple, the mixture fluidized drying in fluidized-bed with thalline and wheat bran obtains the complex microorganism olry bacterial powder; The culture medium prescription of this complex microorganism preparations is: dregs of beans 1.6%, glucose 0.7%, starch 0.2%, yeast powder 0.05%; Potassium hydrogenphosphate 0.01%, potassium primary phosphate 0.02%, manganous sulfate 0.01%, sal epsom 0.05%; Lime carbonate 0.03%, sodium-chlor 0.05%, surplus is a water; Each components contents is mass fraction in this culture medium prescription; The containing quantity of substratum is 70% of a volume in the fermentor tank.
5. according to the method for the said biopurification landscape water of claim 1, it is characterized in that said compound enzymic preparation comprises neutral protease, lypase and cellulase, neutral protease, lypase and cellulase are composite according to the mass ratio of 1～3:1:1～2.
6. according to the method for the said biopurification landscape water of claim 5, it is characterized in that neutral protease is the AS1.398 neutral protease of 30000 U/g for enzyme work; Lypase is the LBK-B400 lypase of 50000 U/g for enzyme work; The enzyme work of cellulase is 20000 U/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102623506A CN102774955A (en) | 2012-07-27 | 2012-07-27 | Biological purification method of landscape water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012102623506A CN102774955A (en) | 2012-07-27 | 2012-07-27 | Biological purification method of landscape water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102774955A true CN102774955A (en) | 2012-11-14 |
Family
ID=47120096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012102623506A Pending CN102774955A (en) | 2012-07-27 | 2012-07-27 | Biological purification method of landscape water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102774955A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355412A (en) * | 2014-11-15 | 2015-02-18 | 北京紫岚风生态环境技术研究院 | Method for biologically purifying eutrophic water body |
| CN104478096A (en) * | 2014-12-13 | 2015-04-01 | 山东永泰化工有限公司 | Water purifying agent for wastewater treatment in rubber accelerator production and wastewater treatment method |
| CN104478095A (en) * | 2014-12-13 | 2015-04-01 | 山东永泰化工有限公司 | Water purifying agent for wastewater treatment in rubber accelerator M production and wastewater treatment method |
| CN104743658A (en) * | 2015-03-12 | 2015-07-01 | 山东省环科院环境科技有限公司 | Wastewater biochemical treatment activated sludge conditioner and preparation method thereof |
| CN114105404A (en) * | 2021-10-27 | 2022-03-01 | 陕西省微生物研究所 | Method for restoring landscape water by using combinational enzyme |
| CN116064309A (en) * | 2022-10-14 | 2023-05-05 | 广西民族大学 | New bacterial strain of pseudomonas and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699213A (en) * | 2005-05-13 | 2005-11-23 | 华东理工大学 | In-situ repairing method for landscape water body |
| CN101085685A (en) * | 2006-06-07 | 2007-12-12 | 上海舜禹环保工程成套技术有限公司 | Landscape water treatment technology |
| CN102557323A (en) * | 2010-10-21 | 2012-07-11 | 江苏绿科生物技术有限公司 | Biological purification method for freshwater aquaculture water environment |
-
2012
- 2012-07-27 CN CN2012102623506A patent/CN102774955A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699213A (en) * | 2005-05-13 | 2005-11-23 | 华东理工大学 | In-situ repairing method for landscape water body |
| CN101085685A (en) * | 2006-06-07 | 2007-12-12 | 上海舜禹环保工程成套技术有限公司 | Landscape water treatment technology |
| CN102557323A (en) * | 2010-10-21 | 2012-07-11 | 江苏绿科生物技术有限公司 | Biological purification method for freshwater aquaculture water environment |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104355412A (en) * | 2014-11-15 | 2015-02-18 | 北京紫岚风生态环境技术研究院 | Method for biologically purifying eutrophic water body |
| CN104478096A (en) * | 2014-12-13 | 2015-04-01 | 山东永泰化工有限公司 | Water purifying agent for wastewater treatment in rubber accelerator production and wastewater treatment method |
| CN104478095A (en) * | 2014-12-13 | 2015-04-01 | 山东永泰化工有限公司 | Water purifying agent for wastewater treatment in rubber accelerator M production and wastewater treatment method |
| CN104478096B (en) * | 2014-12-13 | 2016-08-24 | 山东永泰化工有限公司 | Rubber accelerant wastewater process water purification agent and method of wastewater treatment |
| CN104743658A (en) * | 2015-03-12 | 2015-07-01 | 山东省环科院环境科技有限公司 | Wastewater biochemical treatment activated sludge conditioner and preparation method thereof |
| CN104743658B (en) * | 2015-03-12 | 2016-06-29 | 山东省环科院环境科技有限公司 | A kind of wastewater biochemical processes activated sludge conditioner and preparation method thereof |
| CN114105404A (en) * | 2021-10-27 | 2022-03-01 | 陕西省微生物研究所 | Method for restoring landscape water by using combinational enzyme |
| CN114105404B (en) * | 2021-10-27 | 2024-03-19 | 陕西省微生物研究所 | Method for repairing landscape water by combined enzyme |
| CN116064309A (en) * | 2022-10-14 | 2023-05-05 | 广西民族大学 | New bacterial strain of pseudomonas and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101698539B (en) | Microecological agent for purifying water and preparation method thereof | |
| CN103374524B (en) | Salt-tolerant microbial agent and preparation method thereof | |
| CN110331105A (en) | A kind of sewage treatment composite bacteria agent and preparation method thereof | |
| CN101899397B (en) | Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application | |
| CN103373762B (en) | Biological denitrification method for salt-containing sewage | |
| CN103232944B (en) | Microorganism bacterium agent for straw and excrement mixed composting | |
| CN101333499B (en) | Complex active bacterial biological water purifying a gent and method for preparing same | |
| CN102774955A (en) | Biological purification method of landscape water | |
| CN102557323A (en) | Biological purification method for freshwater aquaculture water environment | |
| CN105907673A (en) | Method for strengthening biochemical water treatment effects by microbial preparation | |
| CN106745828A (en) | A kind of broad-spectrum biological preparation administered for black and odorous water and its application method | |
| CN103374525B (en) | Wastewater treating microbial agent and preparation method thereof | |
| CN102583772A (en) | Microbial preparation comprising mixed microorganisms (bm-s-1), and a biological treatment method for rivers and lakes and a sludge autodigestion process using the same | |
| CN101921709B (en) | Composite high-efficiency microorganism preparation for synergy of sewage treatment plant and synergistic process | |
| CN104891678A (en) | Biological agent used for treating high-concentration waste water produced by amino acid fermentation | |
| CN111547862B (en) | Water body remediation microbial inoculum and preparation method thereof | |
| CN102745821B (en) | Compound microorganism bacterium agent used for sludge reduction, preparation method and application thereof | |
| CN104071902B (en) | Method for treating household wastewater | |
| CN102765853A (en) | Method for treating sewage into recycled water by active organisms | |
| CN109055264A (en) | A kind of microbial bacterial agent and preparation method thereof for holothruian cultures | |
| CN109055252A (en) | A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof | |
| CN101029298A (en) | Production of efficient microbe bacteria combing agent | |
| CN105502805B (en) | Enhancement microbiological multistep treatment sanitary sewage and the processing system and domestic sewage processing method of recycling | |
| CN106967644A (en) | A kind of biological agent for handling glutamic acid fermentation sewage | |
| CN110029066A (en) | A method of utilizing beer waste water culture chlorella |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121114 |