CN109673469A - A kind of preparation method with growth promotion seedling medium - Google Patents
A kind of preparation method with growth promotion seedling medium Download PDFInfo
- Publication number
- CN109673469A CN109673469A CN201811621664.4A CN201811621664A CN109673469A CN 109673469 A CN109673469 A CN 109673469A CN 201811621664 A CN201811621664 A CN 201811621664A CN 109673469 A CN109673469 A CN 109673469A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- function
- preparation
- growth promotion
- seedling medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of preparation methods with growth promotion seedling medium, it is the following steps are included: step 1, the preparation of raw material: in parts by weight, taking 15.0~35.0 parts of perlite, 15.0~35.0 parts of vermiculite, 30.0~70.0 parts of Organic Ingredients, spare；Step 2, the preparation of function strain: selecting, and bacillus amyloliquefaciens are produced in the form of bacterium powder as the function bacterium in fertilizer, function strain, and function bacterial activity is not less than 30,000,000,000/g；Step 3, function bacterium in the step of preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, and fertilizer total weight 0.1~0.5% is added in mixed process two, after mixing, growth promotion function seedling medium is obtained, bacterial activity is not less than 0.3 hundred million/g.The present invention will not damage environment and crop, safe and healthy, pollution-free, be a kind of environmentally protective, Novel seedling raising matrix for being simple and efficient.
Description
Technical field
The present invention relates to nursery field more particularly to a kind of preparation methods with growth promotion seedling medium.
Background technique
The strong sprout for cultivating well developed root system is particularly important to the vigorous growth and resistance promotion of transplanting ensuing crop, at present for strong
The research that seedling is cultivated is concentrated mainly on " influence using different substrates raw material to nursery effect " and " addition hormone is raw to seedling
Two aspects of long promotion ", the utilization of new medium raw material are possible to bring base starting material that can not decompose, and cause environmental pollution
Problem；And the application of hormone then brings various security risks such as environment, health and crop quality in seedling raising process, has simultaneously
It may cause plant growth metabolic disorder, obstacle occurs in late growth.
Summary of the invention
The purpose of the present invention is to solve the shortcomings of the prior art and providing a kind of has the function of growth promotion seedling medium
Preparation method will not damage environment and crop, safe and healthy, pollution-free, and one kind is environmentally protective, is simple and efficient
Novel seedling raising matrix.
In order to achieve the above objectives, the present invention is achieved through the following technical solutions.
A kind of preparation method with growth promotion seedling medium, it is characterised in that: it the following steps are included:
The preparation of raw material: step 1 in parts by weight, takes 15.0~35.0 parts of perlite, vermiculite 15.0~35.0
Part, 30.0~70.0 parts of Organic Ingredients are spare；
The preparation of function strain: step 2 selects bacillus amyloliquefaciens to prepare strain hair as the function bacterium in fertilizer
Strain fermentating liquid is prepared into bacterium powder, function bacterial activity >=30,000,000,000/g in bacterium powder by zymotic fluid；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, and is added in mixed process
Function bacterium in the step of entering fertilizer total weight 0.1~0.5% two, after mixing to get arrive growth promotion function seedling medium,
Bacterial activity >=0.3 hundred million/g.
Further, the preparation of the strain fermentating liquid includes sterilizing, inoculation, fermentation；
During the sterilizing, sky first is carried out to fermentor and is disappeared, be then added after culture medium carry out it is real disappears, sky disappears condition
Are as follows: pressure 0.15MPa, 123 DEG C of temperature, time 1h；Disappear condition in fact are as follows: pressure 0.15MPa, 123 DEG C of temperature, and time 35min；
The process of the inoculation are as follows: completion subject to sterilization is sampled when culture-liquid temp drops to 37 DEG C, utilizes scribing line
Culture and sediments microscope inspection, no viable bacteria growth, sterilizing are completed, then start to be inoculated with, and inoculative proportion (volume ratio) is strain/culture
Liquid 1:120；
It after the inoculation starts 8h, is sampled, using scribing line culture and sediments microscope inspection, detection fermentation has pollution-free；
The fermentation process needs to control revolving speed, ventilatory capacity, fermentation temperature, fermentation time；Revolving speed 6h before fermentation
For 80r/min, 10r hereafter is increased every 2h revolving speed, keeps the revolving speed to fermentation ends after reaching 120r/min；The ventilatory capacity
Are as follows: 8h keeps ventilation quantity 10m before seeding tank ferments3/ h, after every 1h increase ventilation quantity 1m3/ h, until ventilation quantity reaches 15m3/h
When keep fermentation to terminating, whole process keeps tank to be pressed in 0.06Mpa；8h keeps ventilation quantity 30m before fermentor3/ h, after every
1h increases ventilation quantity 5m3/ h, until ventilation quantity reaches 50m3Keep fermentation to terminating when/h, whole process keeps tank to be pressed in
0.06Mpa；
It after the fermentation carries out for 24 hours, is sampled, using scribing line culture and sediments microscope inspection, whether detection fermentation pollutes
With gemma rate, hereafter every 6h carries out primary sample detection, and until gemma rate reaches 95% or more, completion is fermented；
It is described after fermentation, use method for dilute coated plate method sample detection fermentation liquid bacterium amount, bacterium amount be greater than 2.0 ×
109cfu/ml；
The fermentation temperature is 36.5 DEG C -37.5 DEG C.
Further, the Organic Ingredients in the step 1 includes but one of turf, coco bran, decomposed stalk or more
Kind.
Further, the function stem in the step 2 is a bacillus amyloliquefaciens, and preservation registration number is
CGMCC No.15851。
Compared with prior art, the present invention has the advantages that:
Product of the present invention combines compared with existing seedling medium, by active microorganism with seedling medium, strain in matrix
Activity is not less than 0.3 hundred million/g, and can keep the activity of microorganism for a long time.Manufactured growth promotion function seedling medium in this approach
Crop seed can be promoted early to emerge in seedling stage, early take root, crop seedling and root system is promoted quickly to grow vigorously, it is anti-to increase crop
Property, while growing way appearance of the crop within entire breeding time can be improved, crop incidence is reduced, this is because in matrix
The effect of active microorganism can colonize in the soil, constantly play Soluble phosphorus potassium decomposing, dissolves the not Soluble phosphorus potassium in soil, to reach
The effect of promotion plant growth, increase crop yield, crop improvement quality, promotion crop resistance.
Nursery is carried out using growth promotion function seedling medium, since functional microorganism has effects that Soluble phosphorus potassium decomposing, crop
Fertilizers input amount can be reduced after transplanting, increases utilization rate of fertilizer, improve soil physico-chemical property, less fertilizer loss；Meanwhile function
Energy microorganism is considerably increased crop resistance, is used using growth promotion function seedling medium while promotion crop grows up strong and sturdy and grows
It in crop seedling, can not only make crop yield synergy, the use of chemical pesticide can also be reduced, save the cost protects environment,
Increasing both production and income.Therefore, nursery is carried out using growth promotion function seedling medium, can plays and prevents the pollution of the environment to a certain extent
Purpose.
Nursery is carried out using growth promotion function seedling medium, seedling quality can not only be effectively improved, promotes seedlings root hair
It educates and plant strain growth, improves soil after transplanting, enhance the resistance of crop, the investment of chemical fertilizer and pesticide is reduced, with the prior art
Compare, additionally it is possible to avoid the problem that base starting material can not decompose and cause environmental pollution, the application bring environment of hormone, health and
The security risk of crop quality etc., this technology will not damage environment and crop, safe and healthy, pollution-free, be one
Kind environmentally protective, technological innovation, the Novel seedling raising means being simple and efficient.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), preservation registration number CGMCC No.15851；Preservation
Unit: China Committee for Culture Collection of Microorganisms's common micro-organisms center；Depositary institution address: the Chaoyang District, Beijing City North Star
The institute 3 of West Road 1；Preservation date: on June 4th, 2018.
Detailed description of the invention
Fig. 1 is preparation method process flow diagram of the invention；
Specific embodiment
Below with reference to embodiment, the present invention will be further described, but is not limited to the content on specification.
A kind of preparation method with growth promotion seedling medium, it the following steps are included:
The preparation of raw material: step 1 in parts by weight, takes 15.0~35.0 parts of perlite, vermiculite 15.0~35.0
Part, 30.0~70.0 parts of Organic Ingredients are spare；
The preparation of function strain: step 2 selects bacillus amyloliquefaciens as the function bacterium in fertilizer, prepares strain hair
Strain fermentating liquid is prepared into bacterium powder by zymotic fluid, and function bacterial activity is not less than 30,000,000,000/g；
The preparation of strain fermentating liquid: including sterilizing, inoculation, fermentation；
In the sterilization process, sky first is carried out to fermentor and is disappeared, be then added after culture medium carry out it is real disappears, sky disappears condition
Are as follows: pressure 0.15MPa, 123 DEG C of temperature, time 1h；Disappear condition in fact are as follows: pressure 0.15MPa, 123 DEG C of temperature, and time 35min；
The seeded process are as follows: completion subject to sterilization is sampled when culture-liquid temp drops to 37 DEG C, is trained using scribing line
Feeding and sediments microscope inspection, detects the thorough degree of sterilizing of strain, to culture medium scribing line culture without viable bacteria growth and microscopy without viable bacteria
Growth illustrates that sterilizing thoroughly, then starts to be inoculated with, and inoculative proportion (volume ratio) is strain/culture solution 1:120；
It after the inoculation starts 8h, is sampled, using scribing line culture and sediments microscope inspection, whether detection fermentation pollutes；
Crossing, the single colonie form turned out is consistent and microscopy is without miscellaneous bacteria.
The fermentation process needs to control revolving speed, ventilatory capacity, fermentation temperature, fermentation time；Revolving speed 6h before fermentation
For 80r/min, 10r hereafter is increased every 2h revolving speed, keeps the revolving speed to fermentation ends after reaching 120r/min；The ventilation
Amount are as follows: 8h keeps ventilation quantity 10m before seeding tank ferments3/ h, after every 1h increase ventilation quantity 1m3/ h, until ventilation quantity reaches 15m3/
Keep fermentation to terminating when h, whole process keeps tank to be pressed in 0.06Mpa；8h keeps ventilation quantity 30m before fermentor3/ h, it is rear every
Increase ventilation quantity 5m every 1h3/ h, until ventilation quantity reaches 50m3Keep fermentation to terminating when/h, whole process keeps tank to be pressed in
0.06Mpa；
It after the fermentation carries out for 24 hours, is sampled, using scribing line culture and sediments microscope inspection, whether detection fermentation pollutes
With gemma rate, hereafter every 6h carries out primary sample detection, and until gemma rate reaches 95% or more, completion is fermented；
It is described after fermentation, use method for dilute coated plate method sample detection fermentation liquid bacterium amount；Bacterium amount up to 2.0 ×
109Cfu/ml or more.
The fermentation temperature is 36.5 DEG C -37.5 DEG C；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, and is added in mixed process
Function bacterium in the step of entering fertilizer total weight 0.1~0.5% two, after mixing to get arrive growth promotion function seedling medium,
Bacterial activity is not less than 0.3 hundred million/g.
Organic Ingredients in step 1 includes but one of turf, coco bran, decomposed stalk or the quality such as a variety of compounding.
Function stem in step 2 is a bacillus amyloliquefaciens, and preservation registration number is CGMCC No.15851
Illustrate below by specific embodiment.
Specific embodiment 1
A kind of preparation method of growth promotion function seedling medium, includes the following steps:
Step 1, the preparation of raw material: in parts by weight, 25.0 parts of perlite, 25.0 parts of vermiculite, 50.0 parts of turf are taken；
Step 2, the preparation of function strain: the resistance to height of salt tolerant produced with Yunnan Province microbial fermentation engineering research center is used
Temperature simultaneously has the bacillus amyloliquefaciens of Soluble phosphorus potassium decomposing growth promotion function as the function bacterium in fertilizer, and function strain uses bacterium powder
Form produced, bacterial activity be not less than 30,000,000,000/g；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, is added in mixed process
Function bacterium in the step 2 of fertilizer total weight 0.2% obtains educating suitable for the growth promotion function of vegetable cultivation after mixing
Seedling matrix, bacterial activity are not less than 0.3 hundred million/g.
The function stem is provided by Fermentation Research Center Yunnan Co., Ltd., for one plant of solution
Bacillus amyloliquefaciens, preservation registration number are CGMCC No.15851.
Specific embodiment 2
A kind of preparation method of growth promotion function seedling medium, includes the following steps:
Step 1, the preparation of raw material: in parts by weight, 20.0 parts of perlite, 20.0 parts of vermiculite, decomposed stalk are taken
60.0 parts；
Step 2, the preparation of function strain: the resistance to height of salt tolerant produced with Yunnan Province microbial fermentation engineering research center is used
Temperature simultaneously has the bacillus amyloliquefaciens of Soluble phosphorus potassium decomposing growth promotion function as the function bacterium in fertilizer, and function strain uses bacterium powder
Form produced, bacterial activity be not less than 30,000,000,000/g；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, is added in mixed process
Function bacterium in the step 2 of fertilizer total weight 0.5% obtains educating suitable for the growth promotion function of tobacco planting after mixing
Seedling matrix, bacterial activity are not less than 0.3 hundred million/g.
The function stem is provided by Fermentation Research Center Yunnan Co., Ltd., for one plant of solution
Bacillus amyloliquefaciens, preservation registration number are CGMCC No.15851.
Specific embodiment 3
A kind of preparation method of growth promotion function seedling medium, includes the following steps:
Step 1, the preparation of raw material: in parts by weight, 15 parts of perlite, 15 parts of vermiculite, 70 parts of coco bran are taken；
Step 2, the preparation of function strain: the resistance to height of salt tolerant produced with Yunnan Province microbial fermentation engineering research center is used
Temperature simultaneously has the bacillus amyloliquefaciens of Soluble phosphorus potassium decomposing growth promotion function as the function bacterium in fertilizer, and function strain uses bacterium powder
Form produced, bacterial activity be not less than 30,000,000,000/g；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, is added in mixed process
Function bacterium in the step 2 of fertilizer total weight 0.1% obtains educating suitable for the growth promotion function of tobacco planting after mixing
Seedling matrix, bacterial activity are not less than 0.3 hundred million/g.
The function stem is provided by Fermentation Research Center Yunnan Co., Ltd., is one plant of Xie Dian
Afnyloliquefaciens, preservation registration number are CGMCC No.15851.
Specific embodiment 4
A kind of preparation method of growth promotion function seedling medium, includes the following steps:
Step 1, the preparation of raw material: in parts by weight, 35 parts of perlite, 35 parts of vermiculite, 30 parts of coco bran are taken；
Step 2, the preparation of function strain: the resistance to height of salt tolerant produced with Yunnan Province microbial fermentation engineering research center is used
Temperature simultaneously has the bacillus amyloliquefaciens of Soluble phosphorus potassium decomposing growth promotion function as the function bacterium in fertilizer, and function strain uses bacterium powder
Form produced, bacterial activity be not less than 30,000,000,000/g；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, is added in mixed process
Function bacterium in the step 2 of fertilizer total weight 0.5% obtains educating suitable for the growth promotion function of tobacco planting after mixing
Seedling matrix, bacterial activity are not less than 0.3 hundred million/g.
The function stem is provided by Fermentation Research Center Yunnan Co., Ltd., is one plant of Xie Dian
Afnyloliquefaciens, preservation registration number are CGMCC No.15851.
The compound fertilizer of the invention containing active microorganism is tested below:
Test effect of the growth promotion function seedling medium to vegetables nursery of an embodiment of the present invention 1
1. purpose
The dedicated seedling medium of a vegetables is developed, bacillus amyloliquefaciens CGMCC NO. 15851 is added, reaches growth-promoting
Long effect.Emergence rate is up to 85% or more, and for seedling raising process without additional addition fertilizer, plant growing way (plant height, fresh weight) is significantly high
In commercial product.
2. materials and methods
2.1 test material
Matrix: growth promotion function seedling medium, common seedling medium (composition of raw materials and the growth promotion function nursery for not adding bacterium
Discrete phase is same, is distinguished as being not added with function strain) and commercially available common vegetable seedling medium.
Seed: Brassica rapa L (Qingdao 83-1), cauliflower (times Sheng 88), romaine lettuce (Italian romaine lettuce), Lettuce (bright beauty).
2.2 test method
Vegetables are represented as nursery object using Cruciferae, the common nursery that growth promotion function seedling medium is set, does not add bacterium
The three groups of processing of matrix and Vegetable Sold seedling medium, effect of the research functional matrix to vegetables nursery.
3 results and analysis
The plant growth character statistics of different seedling medium processing is as follows:
1 plant growth character of table statistics
As can be seen from the above table, functional matrix can play apparent growth promoting function compared with the generic media that bacterium is not added,
It is compared with commercially available matrix, even more significant difference, there is extremely strong growth-promoting effect.
Test influence of the growth promotion function seedling medium of two embodiment of the present invention 2 to tobacco growing
1, test objective and meaning
During tobacco planting, many tobacco seedlings are to will appear as in seedling stage sick and weak, and field growing is bad after transplanting, survives
Rate is low, and case fatality rate is high.Therefore, the cultivation of tobacco strong sprout is undergrowth after reducing tobacco seedlings transplanting, the high most effective side of case fatality rate
Method.Tobacco seedling uses matrix floating seedlings mostly at present, and floating seedlings intensive manufacture is provided to the large-scale cultivation of tobacco seedlings
Good condition, but the strong seedling cultivation of tobacco seedlings also needed to further strengthen.Yunnan Yunye Fertilizer Co., Ltd. grinds
The growth promotion function seedling medium of system, is added to the microorganism fungus kind with growth promotion function, crop seed can be promoted early to go out
Seedling is early taken root, and crop seedling and root system is promoted quickly to grow vigorously, and increases crop resistance, improves crop within entire breeding time
Growing way appearance, reducing crop incidence has positive influence, while will not damage to environment and crop, it is safe and healthy,
It is pollution-free, tobacco planting is had a very big significance.
2, test material and method
2.1 test material
This test material therefor be Yunnan Yunye Fertilizer Co., Ltd. production growth promotion function seedling medium, when
Ground conventional seedbed system matrix, tobacco seed used are K326.
2.2 experimental design
This test designs two processing altogether:
Functional matrix: being uniformly packed into drift disk by processing group, and implantation seed is placed and carries out nursery in tobacco seedling culture canopy, sees
Field conditions after examining tobacco seedlings emergence rate, tobacco seedlings growing way and transplanting.
Control group: nursery is carried out using local conventional substrate.
3, survey item and method
3.1 emergence rates and emergence standard survey
Emergence rate: 5d is investigated after emergence, emergence rate=(every disk seedling number/often circle or whirl in the air number) * 100%.
Strong sprout rate: strong sprout rate=(strong sprout strain number/seedling strain number) * 100%.
Strong sprout standard: seedling stalk is light green, flexibility is good, stem girth 1.8-2.0cm, the high 8-12cm of stem, blade 7-8 piece, leaf
Color is light green or green, well developed root system, no disease and pests harm, and seedling is uniform in size, healthy and strong, neat and consistent.
The investigation of 3.2 tobacco seedlings economical characters
Economical character after investigating different disposal seedling, 5 plants of tobacco seedlings of every disk random inspection investigate height of seedling, stem thickness, maximum leaf
It is long, strong sprout rate, survival rate after transplanting.
3.3 tobacco seedlings Pests
A situation arises for investigation different disposal disease species survey and disease.
The investigation of 3.4 crop fields
3.4.1 breeding time is investigated
The transplanting time of two processing is investigated, 50% cigarette strain enters group, prosperous growth, buddings, binds the time pruned.
3.4.2 economical character is investigated
Each processing randomly selects 15 plants of investigation plant heights of representative cigarette strain, stem girth, three pitch, upper, middle and lower positions
The long leaf width of leaf.
3.4.3 yield and quality is investigated
Investigate and compare yield and quality after baking.
4, result and analysis
4.1 seedlings investigations and analysis
2 seedlings investigation table of table
| Processing | Sowing time | Seeding stage | The small cross phase | The grand cross phase | Seedling stage |
| Matrix treatments | 26 days 2 months | March 16 | March 28 | April 4 | April 20 |
| Conventional treatment | 26 days 2 months | March 21 | April 1 | April 8 | April 24 |
The seeding stage that growth promotion matrix carries out nursery as seen from the above table shifts to an earlier date 5d than conventional, into the time of small cross phase
3d in advance, into the time advance 4d of grand cross phase, seedling stage shifts to an earlier date 4d.
4.2 emergence rates, planting percent investigation and analysis
3 emergence rate of table, planting percent application form
| Processing | Emergence rate | Planting percent | Strong sprout rate |
| Matrix treatments | 97 | 96.1 | 96.2 |
| Conventional treatment | 95 | 97.7 | 96.6 |
97% > conventional substrate of emergence rate emergence rate 95% of growth promotion substrate seedling as seen from the above table, planting percent
96.1% < conventional treatment 97.7%, 96.2% < conventional treatment of strong sprout rate 96.6%.
The investigation of 4.3 tobacco seedlings quality and analysis
4 tobacco seedlings quality application form of table
| Processing | Seeding stage | The small cross phase | The grand cross phase |
| Matrix treatments | It is light green | It is light green | It is green |
| Conventional treatment | It is light green | It is light green | It is green |
Difference is not shown in the quality in tobacco seedlings each period as seen from the above table.
The investigation of 4.4 tobacco seedlings economical characters and analysis
5 tobacco seedlings economical character application form of table
The tobacco seedling growth of growth promotion matrix is rapider than conventional as seen from the above table, and tobacco seedlings leaf when the small cross phase is long, leaf
Wide, stem height is bigger than routine.The tobacco seedlings leaf length of grand cross phase, leaf width, stem is high, stem girth is bigger than Routine control.Seedling stage
Leaf length, leaf width, stem height are also all bigger than Routine control.
4.5 Pests and analysis
6 Pests table of table
| Pest and disease damage | Balck shank | Samping off | Aphid | Moth |
| Matrix treatments | 3% | 5% | 12% | 9% |
| Conventional treatment | 5% | 9% | 12% | 10% |
The balck shank of growth promotion matrix tobacco seedlings and samping off disease incidence are less than conventional tobacco seedlings as seen from the above table, aphid and fly
The generation of moth is related to the insect prevention measure of the permeability of roc, which is not discussed.
The investigation of 4.6 growing stages and analysis
7 growing stage application form of table
Growth promotion matrix tobacco seedlings are identical with conventional tobacco seedlings transplanting time as seen from the above table, and growth promotion matrix tobacco seedlings enter group
The time of phase than conventional 2 days in advance, into the prosperous growth phase time than conventional 5 days in advance, into squaring period time than conventional
3 days in advance.
The investigation of 4.7 crop field economical characters and analysis
8 Agronomic characteristic application form of table
Performance of the growth promotion matrix tobacco seedlings in crop field is slightly better than conventional tobacco seedlings as seen from the above table, the plant height in each period, stem
It encloses and the length and width of tobacco leaf all shows different difference, the plant height in each period is higher than conventional tobacco seedlings, but stem girth is each in each period
Portion is identical, and the single plant number of sheets is more than conventional in group's phase, prosperous growth phase, and fewer than conventional after buddingging and binding, leaf is long, leaf width is slightly smaller than
Routine control.
5, conclusion and discussion
It can be concluded that by experiment
1) growth promotion matrix seedling-growing time is short, and emergence rate is high, and early period, tobacco seedling growth was rapid, and later period tobacco seedlings compare conventional treatment
Good, growth promotion matrix can improve the activity of matrix because being added to growth promotion microbial bacterial agent, promote seedling growth and development.
2) the tobacco seedlings high survival rate of growth promotion matrix, strong sprout rate is good, can be improved the resistance of tobacco seedlings.And current year tests
Region rainwater early period is less, and weather is warmmer, and growth promotion matrix tobacco seedlings and conventional tobacco seedlings suffer from different degrees of high temperature and irradiation,
But although the tobacco seedlings part limb of growth promotion matrix is shrivelled, and the later period still survives, and illustrates the tobacco seedlings resistance of growth promotion matrix
Preferably.
3) quality of growth promotion matrix tobacco seedlings and conventional tobacco seedlings quality determine not distinguish significantly by naked eyes.
4) performance in growth promotion matrix tobacco seedlings each period is better than conventional tobacco seedlings, illustrates that growth promotion matrix can promote tobacco seedlings
Growth and development.
5) breeding time of growth promotion matrix tobacco seedlings shifts to an earlier date compared with conventional tobacco seedlings breeding time, and cigarette strain growth and development is rapid.
6) plant height of growth promotion matrix tobacco seedlings is higher, and blade quantity is more intensive, and the length and width of upper middle part blade are preferable.
Can obtain in summary: growth promotion matrix can promote the emergence rate and survival rate of tobacco seedlings nursery, can be improved tobacco seedlings
The resistance in seedling stage can promote the breeding time of field period cigarette strain to be in progress and develop, cigarette strain height growth can be promoted to develop.
It is an object of the present invention to solve the deficiency of the existing technology and provide a kind of seedling mediums with growth promotion function
And preparation method thereof, growth promotion functional activity microorganism is efficiently applied in seedling medium production, growth promotion function is made and educates
Seedling substrate products, for crop seedling early growth and quick tiller, grow vigorously, resistance promotion have good facilitation, can be effective
Promote the growth and development of crops seedling stage, and beneficial effect is brought to the growth of breeding time entire after transplanting, promotes economic benefit
It is promoted.
Applicant combines the reality of Crop Diseases in Yunnan production, the salt tolerant produced with Yunnan Province microbial fermentation engineering research center
High temperature resistant simultaneously has the bacillus amyloliquefaciens of Soluble phosphorus potassium decomposing growth promotion function as the function bacterium in fertilizer, and functional activity is micro-
Biology is combined with seedling medium, produces growth promotion function seedling medium, table of field test results are bright, and the product is for promoting to make
The early emergence of species, early takes root, crop seedling and root system is promoted quickly to grow vigorously, increase crop resistance, improves crop whole
Growing way appearance in a breeding time, reducing crop incidence has positive influence, while will not damage to environment and crop,
It is safe and healthy, pollution-free, for the green, organic of modern agriculture, Harmless development tool very big significance.
Obviously, above embodiment of the invention be only to clearly illustrate example of the present invention, and not be
Restriction to embodiments of the present invention.For those of ordinary skill in the art, on the basis of the above description also
It can make other variations or changes in different ways.Here all embodiments can not be exhaustive.It is all to belong to this
The obvious changes or variations that the technical solution of invention is extended out are still in the scope of protection of the present invention.
Claims (4)
1. a kind of preparation method with growth promotion seedling medium, it is characterised in that: it the following steps are included:
The preparation of raw material: step 1 in parts by weight, takes 15.0~35.0 parts of perlite, 15.0~35.0 parts of vermiculite, has
It is 30.0~70.0 parts of machine raw material, spare；
Step 2, the preparation of function strain: selecting bacillus amyloliquefaciens as the function bacterium in fertilizer and prepare strain fermentating liquid,
Strain fermentating liquid is prepared into bacterium powder, function bacterial activity >=30,000,000,000/g in bacterium powder；
Step 3, the preparation of growth promotion function seedling medium: the raw material in step 1 is mixed, and fertilizer is added in mixed process
Expect total weight 0.1~0.5% the step of two in function bacterium, after mixing to get arrive growth promotion function seedling medium, strain
Activity >=0.3 hundred million/g.
2. a kind of preparation method with growth promotion seedling medium according to claim 1, it is characterised in that: described
The preparation of strain fermentating liquid includes sterilizing, inoculation, fermentation；
During the sterilizing, sky first is carried out to fermentor and is disappeared, be then added after culture medium carry out it is real disappears, sky disappears condition are as follows:
Pressure 0.15MPa, 123 DEG C of temperature, time 1h；Disappear condition in fact are as follows: pressure 0.15MPa, 123 DEG C of temperature, and time 35min；
The process of the inoculation are as follows: completion subject to sterilization is sampled when culture-liquid temp drops to 37 DEG C, is cultivated using scribing line
And sediments microscope inspection, no viable bacteria growth, sterilizing are completed, then start to be inoculated with, inoculative proportion (volume ratio) is strain/culture solution 1:
120。
It after the inoculation starts 8h, is sampled, using scribing line culture and sediments microscope inspection, detection fermentation has pollution-free；
The fermentation process needs to control revolving speed, ventilatory capacity, fermentation temperature, fermentation time；Revolving speed 6h before fermentation is
Hereafter 80r/min increases 10r every 2h revolving speed, keeps the revolving speed to fermentation ends after reaching 120r/min；The ventilatory capacity
Are as follows: 8h keeps ventilation quantity 10m before seeding tank ferments3/ h, after every 1h increase ventilation quantity 1m3/ h, until ventilation quantity reaches 15m3When/h
Keep fermentation to terminating, whole process keeps tank to be pressed in 0.06Mpa；8h keeps ventilation quantity 30m before fermentor3/ h, after every 1h
Increase ventilation quantity 5m3/ h, until ventilation quantity reaches 50m3Keep fermentation to terminating when/h, whole process keeps tank to be pressed in 0.06Mpa；
It after the fermentation carries out for 24 hours, is sampled, using scribing line culture and sediments microscope inspection, whether detection fermentation pollutes and bud
Spore rate, hereafter every 6h carries out primary sample detection, and until gemma rate reaches 95% or more, completion is fermented；
Described to use method for dilution coated plate method sample detection fermentation liquid bacterium amount after fermentation, bacterium amount is greater than 2.0 × 109cfu/
ml；
The fermentation temperature is 36.5 DEG C -37.5 DEG C.
3. a kind of preparation method with growth promotion seedling medium according to claim 1, it is characterised in that: described
Organic Ingredients in step 1 includes but one of turf, coco bran, decomposed stalk or a variety of.
4. a kind of preparation method with growth promotion seedling medium according to claim 1, it is characterised in that: described
Function stem in step 2 is a bacillus amyloliquefaciens, and preservation registration number is CGMCC No.15851.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811621664.4A CN109673469A (en) | 2018-12-28 | 2018-12-28 | A kind of preparation method with growth promotion seedling medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811621664.4A CN109673469A (en) | 2018-12-28 | 2018-12-28 | A kind of preparation method with growth promotion seedling medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109673469A true CN109673469A (en) | 2019-04-26 |
Family
ID=66190726
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811621664.4A Pending CN109673469A (en) | 2018-12-28 | 2018-12-28 | A kind of preparation method with growth promotion seedling medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109673469A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731248A (en) * | 2019-09-29 | 2020-01-31 | 云南省微生物发酵工程研究中心有限公司 | biological seedling culture substrate with clubroot prevention and treatment function and preparation method thereof |
| CN110754325A (en) * | 2019-11-04 | 2020-02-07 | 李剑峰 | EPS composite rock surface repairing matrix |
| CN113767833A (en) * | 2020-06-09 | 2021-12-10 | 杭州元成规划设计集团有限公司 | Slope growth substrate and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747017A (en) * | 2012-07-16 | 2012-10-24 | 南京农业大学 | Bacillus amyloliquefaciens and application thereof |
| KR20150065116A (en) * | 2013-12-04 | 2015-06-12 | 오윤성 | Culture medium for growing pine mushroom and method for producing the same |
| CN105746033A (en) * | 2016-03-02 | 2016-07-13 | 江苏省农业科学院 | Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables |
| CN107099465A (en) * | 2017-04-12 | 2017-08-29 | 安徽省农业科学院茶叶研究所 | For the growth-promoting bacterial strain and its microorganism seedling medium for promoting cuttage tea shoot to take root |
| CN108124728A (en) * | 2017-12-29 | 2018-06-08 | 河南科技学院 | A kind of breeding matrix and preparation method for promoting cotton fast-growth |
| CN109020641A (en) * | 2018-07-10 | 2018-12-18 | 云南省微生物发酵工程研究中心有限公司 | A kind of biological organic fertilizer and preparation method thereof with prophyiaxis and promoting growth function |
-
2018
- 2018-12-28 CN CN201811621664.4A patent/CN109673469A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747017A (en) * | 2012-07-16 | 2012-10-24 | 南京农业大学 | Bacillus amyloliquefaciens and application thereof |
| KR20150065116A (en) * | 2013-12-04 | 2015-06-12 | 오윤성 | Culture medium for growing pine mushroom and method for producing the same |
| CN105746033A (en) * | 2016-03-02 | 2016-07-13 | 江苏省农业科学院 | Application of bacillus amyloliquefaciens B-1619 in aspect of promoting growth of greenhouse vegetables |
| CN107099465A (en) * | 2017-04-12 | 2017-08-29 | 安徽省农业科学院茶叶研究所 | For the growth-promoting bacterial strain and its microorganism seedling medium for promoting cuttage tea shoot to take root |
| CN108124728A (en) * | 2017-12-29 | 2018-06-08 | 河南科技学院 | A kind of breeding matrix and preparation method for promoting cotton fast-growth |
| CN109020641A (en) * | 2018-07-10 | 2018-12-18 | 云南省微生物发酵工程研究中心有限公司 | A kind of biological organic fertilizer and preparation method thereof with prophyiaxis and promoting growth function |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731248A (en) * | 2019-09-29 | 2020-01-31 | 云南省微生物发酵工程研究中心有限公司 | biological seedling culture substrate with clubroot prevention and treatment function and preparation method thereof |
| CN110731248B (en) * | 2019-09-29 | 2021-11-16 | 云南省微生物发酵工程研究中心有限公司 | Biological seedling culture substrate with clubroot disease prevention and treatment function and preparation method thereof |
| CN110754325A (en) * | 2019-11-04 | 2020-02-07 | 李剑峰 | EPS composite rock surface repairing matrix |
| CN113767833A (en) * | 2020-06-09 | 2021-12-10 | 杭州元成规划设计集团有限公司 | Slope growth substrate and preparation method thereof |
| CN113767833B (en) * | 2020-06-09 | 2023-07-04 | 杭州元成规划设计集团有限公司 | Slope growth matrix and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109971680B (en) | Preparation and application of compound microbial agent with disease-resistant and growth-promoting functions | |
| CN112980717B (en) | Solid bacillus microbial agent and preparation method thereof | |
| CN103329720B (en) | Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion | |
| CN105331567B (en) | A kind of bacillus amyloliquefaciens and promoting growth of plants preparation and application | |
| CN103214292A (en) | Plant cultivation medium and its preparation method | |
| CN103518781B (en) | Core bio-control bacteria and organic fertilizer containing the core bio-control bacteria | |
| US20170367351A1 (en) | Mixotrophic chlorella-based composition, and methods of its preparation and application to plants | |
| EP3232790A1 (en) | Mixotrophic chlorella-based composition, and methods of its preparation and application to plants | |
| CN111411063A (en) | Bacillus composite microbial inoculum with function of preventing and treating tobacco soil-borne diseases and preparation method thereof | |
| CN109673469A (en) | A kind of preparation method with growth promotion seedling medium | |
| CN107371724B (en) | Spinach cultivation medium and cultivation method | |
| CN118165892B (en) | Pseudomonas composite microbial agent and application thereof | |
| CN107418906B (en) | A kind of composite bacteria agent and its application improving color green pepper yield and anti-root rot | |
| WO2017218896A1 (en) | Microalgae-based composition, and methods of its preparation and application to plants | |
| CN105439657B (en) | A kind of preparation method of the special biologic organic fertilizer of resisting repeated stubbles of strawberry | |
| CN106518185A (en) | Special composite microbial fertilizer with functions of strengthening seedlings and promoting roots for tobaccos, and preparation method thereof | |
| CN106148250B (en) | One plant of Costa Rica streptomyces actinomyces and application thereof | |
| CN110205249B (en) | Method for promoting plant growth and alternaria alternate fungus used by same | |
| CN109937640A (en) | A method of enhancing alfalfa resistance | |
| CN114946600A (en) | Vegetable seedling raising substrate and preparation method thereof | |
| CN109609419B (en) | Zollinia bacteria and application thereof in improving stress resistance of plants | |
| CN108575811B (en) | Preparation method of fungus grass fermentation bed | |
| CN113897316A (en) | Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application of functional melon and fruit seedling biological matrix | |
| CN107216186A (en) | Production method and application process with disease-resistant growth-promoting functions functional biological organic fertilizer | |
| CN108504586B (en) | Paenibacillus softening and straw decomposing agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190426 |