CN108575811B - Preparation method of fungus grass fermentation bed - Google Patents

Preparation method of fungus grass fermentation bed Download PDF

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CN108575811B
CN108575811B CN201810360470.7A CN201810360470A CN108575811B CN 108575811 B CN108575811 B CN 108575811B CN 201810360470 A CN201810360470 A CN 201810360470A CN 108575811 B CN108575811 B CN 108575811B
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grass
fermentation bed
padding
fungus
fermentation
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CN108575811A (en
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孙志宏
徐雅皙
张会
郝柳柳
姜深
林加明
胡仕恒
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Yanan University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K31/00Housing birds
    • A01K31/04Dropping-boards; Devices for removing excrement
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00Housing animals; Equipment therefor
    • A01K1/015Floor coverings, e.g. bedding-down sheets ; Stable floors
    • A01K1/0152Litter
    • A01K1/0155Litter comprising organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K31/00Housing birds
    • A01K31/002Poultry cages, e.g. transport boxes
    • A01K31/007Floors

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  • Environmental Sciences (AREA)
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  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

The invention discloses a preparation method of a fungus grass fermentation bed, which comprises the following steps: preparing a special bacterium liquid for a fermentation bed, preparing fungus grass padding, weighing the rest padding, uniformly mixing the padding to prepare a fungus grass padding mixture, preparing a fungus grass fermentation bed finished product, transferring the fungus grass fermentation bed finished product into a colorful pheasant breeding house, and flatly paving the fungus grass fermentation bed on the ground of the breeding house to obtain the fungus grass fermentation bed. Solves the problems that the traditional fermentation bed padding in the prior art is difficult to obtain, has high cost and poor fermentation effect, and the feces in the prior breeding technology are harmful and difficult to treat.

Description

Preparation method of fungus grass fermentation bed
Technical Field
The invention belongs to the technical field of cultivation, and relates to a preparation method of a fungus grass fermentation bed.
Background
Malodorous gases such as ammonia gas and hydrogen sulfide emitted by livestock and poultry manure can make the raised livestock and poultry susceptible to respiratory diseases, so that the problems of increased mortality, reduced organism immunity, reduced production performance, reduced meat quality and the like are caused, and in addition, the environment of a breeding house can be seriously polluted. In 2010, the discharge amount of livestock and poultry excrement in China reaches 45 hundred million tons, the discharge amount of the livestock and poultry excrement is increased along with the development of the breeding industry, and if the large excrement is not treated properly, serious ecological environment problems and social problems are caused. Difficulty in handling livestock and poultry manure: the commodity value of the livestock and poultry manure is low; secondly, the livestock and poultry manure has high water content, high viscosity, more impurities, large smell and high processing difficulty; and thirdly, the livestock and poultry manure is high in production speed and large in quantity, and wastes time and labor when being treated.
The livestock and poultry manure can cause serious harm to livestock, poultry and environment, great loss is brought to the breeding industry and the ecological environment, excessive manpower, material resources and financial resources are consumed in the treatment process of the livestock and poultry manure, the breeding cost is increased, and the economic benefit is reduced.
The fermentation bed culture technology can solve various hazards brought by livestock and poultry manure, beneficial bacteria in the fermentation bed can decompose the manure produced by livestock and poultry, and residual nutrition in the livestock and poultry manure is utilized to produce beneficial mycoprotein, so that the emission of harmful gases such as ammonia gas and hydrogen sulfide is effectively controlled, the air environment of a culture house is improved, and the probability of respiratory diseases of the livestock and poultry caused by the harmful gases is reduced.
But the bedding material of current traditional fermentation bed adopts sawdust mostly, and in recent years, the country strictly limits the volume of cutting down timber, to the recycle of sawdust among the wood working industry to and the cost problem that uses the sawdust in a large number and bring, make the taking of sawdust have some realistic difficulties. And the sawdust adopted has the problems of high cost, poor fermentation effect and the like.
At present, a large amount of funguses are planted in most regions of China, the fungus and grass technology is introduced from the national fungus and grass engineering technology research center of Fujian agriculture and forestry university in 2013, 20 fungus and grass demonstration bases are built in Yanan city for the first time, 15 families of Yuan enterprises and professional cooperative society in fungus and grass industry are cultivated, the planting area reaches 4 ten thousand mu, 103 sheds of seedlings are cultivated, the problems of overwintering and seedling external adjustment of the fungus and grass in northern regions are solved, and 13 counties and regions in the whole city are covered. Striving for 2020, the planting area of the fungi and the grass in the whole market reaches more than 5-10 ten thousand mu. The Juncao has abundant resources in Yanan areas, and has considerable development prospect of industrial chains.
Disclosure of Invention
The invention aims to provide a preparation method of a fungus grass fermentation bed, which solves the problems that the traditional fermentation bed is difficult to obtain padding, high in cost and poor in fermentation effect in the prior art, and feces are harmful and difficult to treat in the prior art.
The technical scheme of the invention is that the preparation method of the grass and fungus fermentation bed specifically comprises the following steps:
step 1, preparing special bacteria liquid for fermentation bed
Step 2, preparing the fungus grass padding
Selecting fresh grass, crushing the fresh grass into a filament shape with the length of about 3-5 cm by using a thread rolling machine, spreading the crushed grass at a ventilated place, and airing the moisture on the surface until the grass is not agglomerated;
step 3, weighing the rest padding materials
Weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 10-50% of grass, 40-72% of sawdust, 5-9% of wheat bran and 5-9% of corn flour, wherein the sum of the mass percentages of the padding is 100%;
step 4, uniformly mixing all padding to prepare a fungus grass padding mixture
Step 5, preparing a finished product of the fungus grass fermentation bed
Stacking the fungus grass padding mixture prepared in the step (4) into a cone, covering a breathable film, and performing stacking fermentation for 5-7 days to obtain a fungus grass fermentation bed finished product;
and 6, transferring the finished product of the fungus grass fermentation bed prepared in the step 5 into a pheasant breeding house, and paving the pheasant breeding house on the ground to obtain the fungus grass fermentation bed.
The present invention is also characterized in that,
the step 1 specifically comprises the following steps:
step 1.1, respectively weighing 5-6% of EM (effective microorganism) bacterial powder, 11-12% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterial powder, the brown sugar powder and the sterile water is 100%;
step 1.2, putting the brown sugar powder weighed in the step 1.1 into a beaker, adding a small amount of hot water for dissolving, adding 80-85% of sterile water after dissolving, stirring uniformly and cooling;
step 1.3, putting the EM bacterial powder measured in the step 1.1 into another beaker, and adding 5-10% of sterile water for dissolving;
and step 1.4, pouring the brown sugar dissolved in the step 1.2 and the bacterial liquid dissolved in the step 1.3 into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade place for fermentation for 3-5 days to obtain the bacterial liquid special for the fermentation bed.
The step 4 specifically comprises the following steps:
step 4.1, taking the special bacterial liquid for the fermentation bed prepared in the step 1, and carrying out fermentation according to the special bacterial liquid for the fermentation bed: diluting with sterile water in a volume ratio of 1: 50-1: 40;
and 4.2, adding the bacterial liquid special for the fermentation bed diluted in the step 4.1 into the mixed padding weighed in the step 3, and adjusting the water content of the padding mixture to 40-50% to obtain the grass and fungus padding mixture.
Step 4.2 specifically comprises the following steps:
step 4.2.1, uniformly mixing the sawdust and the fungus grass weighed in the step 3;
step 4.2.2, adding the wheat bran and the corn flour weighed in the step 3, and uniformly mixing to prepare a mixed padding;
and 4.2.3, slowly adding the special bacterial liquid for the fermentation bed diluted in the step 4.1 into the mixed padding prepared in the step 4.2.2, and continuously stirring the mixture without agglomerating the mixture until the water content of the mixture is adjusted to be 40-50%.
And 5, randomly inserting 10-15 holes with the aperture of 4-6 cm and the depth of 15-20 cm into the side surface of the cone of the conical accumulation object before covering the air-permeable film.
In step 5, the temperature of the stacking fermentation is 23-27 ℃.
In the step 6, the finished product of the fungus grass fermentation bed is paved with the thickness of 25 cm-35 cm.
The beneficial effect of the invention is that,
1. a method for preparing a fungus grass fermentation bed adopts a fungus grass part to replace the traditional fermentation bed padding sawdust so as to reduce the padding cost of the fermentation bed, improve the nutrition and enhance the fermentation effect. The prepared zymophyte liquid has low cost and a plurality of beneficial strains, the bacterium liquid is added into padding to prepare the fungus and grass fermentation bed, and the beneficial strains can absorb sufficient nutrition in the fungus and grass fermentation bed and propagate massively under the conditions of proper temperature, humidity, PH and the like of the fermentation bed. After early fermentation, the fungus grass fermentation bed is moved into a breeding house and is paved on the ground of the breeding house in a thickness of 30cm, and livestock and poultry are bred on the fermentation bed. The beneficial bacteria in the fermentation bed can decompose the excrement discharged by the livestock and poultry, and the residual nutrition in the excrement is utilized to generate beneficial mycoprotein, so that the discharge of harmful gases such as ammonia gas and hydrogen sulfide can be effectively controlled, and the harm of the harmful gases to the livestock, poultry and the environment is reduced. Meanwhile, the excrement is decomposed, and the cultured livestock and poultry can also use the mixed padding of the edible fungus grass complex fermentation bed and the decomposed excrement, so that the livestock and poultry contain great nutritive value, and the production performance and the organism immunity of the livestock and poultry can be improved. The invention solves the problem of difficult treatment of the livestock and poultry manure, reduces the environmental pollution, reduces the breeding cost, saves the labor force, improves the organism immunity and the meat quality of the livestock and poultry, and increases the economic benefit of livestock and poultry breeding;
2. the preparation method of the fungus grass fermentation bed has the advantages of low planting cost of the material fungus grass, high yield, short growth period, strong stress resistance and high content of crude protein and sugar. Various characteristics and advantages of the fungus grass show that the fungus grass can partially replace the sawdust of the traditional fermentation bed padding;
3. the prepared fungus grass fermentation bed can solve various hazards brought by livestock and poultry manure, beneficial bacteria in the fermentation bed can decompose the manure produced by livestock and poultry, and beneficial mycoprotein is produced by utilizing residual nutrition in the manure of livestock and poultry, so that the discharge of harmful gases such as ammonia gas and hydrogen sulfide is effectively controlled, the air environment of a breeding house is improved, and the probability of respiratory diseases of the livestock and poultry caused by harmful gases is reduced. Meanwhile, the excrement is decomposed, and the cultured livestock and poultry can also use the mixed bedding of the edible fungus grass fermentation bed and the decomposed excrement, so that the livestock and poultry contain great nutritive value, and the production performance and the organism immunity of the livestock and poultry can be improved.
Drawings
FIG. 1 is a graph showing the temperature change of a normal fermentation bed in a bedding fermenting and ripening process in the former 22 days in winter and summer in the preparation method of a fermented bed for herbs of the present invention;
FIG. 2 is a diagram of germination of a control group in a toxicity test of fermentation bed padding of each group to plants in a preparation method of a fungus grass fermentation bed;
FIG. 3 is a diagram of germination of a traditional group in toxicity test of fermentation bed padding to plants in various groups in a preparation method of a fungus grass fermentation bed;
FIG. 4 is a diagram showing the germination of 10% of the fungal grass group in the toxicity test of the fungal grass fermentation bed padding to plants in each group in the preparation method of the fungal grass fermentation bed;
FIG. 5 is a diagram showing the germination of 30% of the fungal grass group in the toxicity test of the fungal grass fermentation bed padding to plants in each group in the preparation method of the fungal grass fermentation bed;
FIG. 6 is a diagram showing germination conditions of 50% of the fungal grass groups in the toxicity test of each group of fermentation bed padding on plants in the preparation method of the fungal grass fermentation bed.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
The invention relates to a preparation method of a fungus grass fermentation bed, which comprises the following steps:
step 1, preparing special bacteria liquid for fermentation bed
Step 2, preparing the fungus grass padding
Selecting fresh grass, crushing the fresh grass into a filament shape with the length of about 3-5 cm by using a thread rolling machine, spreading the crushed grass at a ventilated place, and airing the moisture on the surface until the grass is not agglomerated;
step 3, weighing the rest padding materials
Weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 10-50% of grass, 40-72% of sawdust, 5-9% of wheat bran and 5-9% of corn flour, wherein the sum of the mass percentages of the padding is 100%;
step 4, uniformly mixing all padding to prepare a fungus grass padding mixture
Step 5, preparing a finished product of the fungus grass fermentation bed
Stacking the fungus grass padding mixture prepared in the step (4) into a cone, covering a breathable film, and performing stacking fermentation for 5-7 days to obtain a fungus grass fermentation bed finished product;
and 6, transferring the finished product of the fungus grass fermentation bed prepared in the step 5 into a pheasant breeding house, and paving the pheasant breeding house on the ground to obtain the fungus grass fermentation bed.
The step 1 specifically comprises the following steps:
step 1.1, respectively weighing 5-6% of EM (effective microorganism) bacterial powder, 11-12% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterial powder, the brown sugar powder and the sterile water is 100%;
step 1.2, putting the brown sugar powder weighed in the step 1.1 into a beaker, adding a small amount of hot water for dissolving, adding 80-85% of sterile water after dissolving, stirring uniformly and cooling;
step 1.3, putting the EM bacterial powder measured in the step 1.1 into another beaker, and adding 5-10% of sterile water for dissolving;
and step 1.4, pouring the brown sugar dissolved in the step 1.2 and the bacterial liquid dissolved in the step 1.3 into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade place for fermentation for 3-5 days to obtain the bacterial liquid special for the fermentation bed.
The step 4 specifically comprises the following steps:
step 4.1, taking the special bacterial liquid for the fermentation bed prepared in the step 1, and carrying out fermentation according to the special bacterial liquid for the fermentation bed: diluting with sterile water in a volume ratio of 1: 50-1: 40;
and 4.2, adding the bacterial liquid special for the fermentation bed diluted in the step 4.1 into the mixed padding weighed in the step 3, and adjusting the water content of the padding mixture to 40-50% to obtain the grass and fungus padding mixture.
Step 4.2 specifically comprises the following steps:
step 4.2.1, uniformly mixing the sawdust and the fungus grass weighed in the step 3;
step 4.2.2, adding the wheat bran and the corn flour weighed in the step 3, and uniformly mixing to prepare a mixed padding;
and 4.2.3, slowly adding the special bacterial liquid for the fermentation bed diluted in the step 4.1 into the mixed padding prepared in the step 4.2.2, and continuously stirring the mixture without agglomerating the mixture until the water content of the mixture is adjusted to be 40-50%.
And 5, randomly inserting 10-15 holes with the aperture of 4-6 cm and the depth of 15-20 cm into the side surface of the cone of the conical accumulation object before covering the air-permeable film.
In the step 5, the temperature of the stacking fermentation is 23-27 ℃.
In the step 6, the finished product of the fungus grass fermentation bed is paved with the thickness of 25 cm-35 cm.
According to the preparation method of the fungus grass fermentation bed, an innovative method that fungus grass partially replaces sawdust is adopted, and the fungus grass fermentation bed with excellent fermentation effect and cultivation effect is manufactured by combining the optimal replacement proportion, so that the problems of economic loss and environmental pollution of the traditional cultivation industry are solved, the cost and resources are saved on the original traditional fermentation bed, and the cultivation effect of the traditional fermentation bed is optimized.
Example 1
Preparing special bacterial liquid for a fermentation bed: respectively weighing 5% of EM (effective microorganism) bacterium powder, 12% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterium powder, the brown sugar powder and the sterile water is 100%, putting the brown sugar powder into a beaker, adding a small amount of hot water for dissolving, adding 83% of sterile water after dissolving, uniformly stirring, cooling, putting the EM bacterium powder into another beaker, adding 8% of sterile water for dissolving, pouring the dissolved brown sugar water and bacterium liquid into a fermentation bottle, adding the balance of sterile water, and then putting the fermentation bottle in a room for fermenting for 4 days to obtain the bacterium liquid special for the fermentation bed;
preparing a fungus grass padding: selecting fresh grass, crushing the fresh grass into filaments with the length of about 4cm by using a thread rolling machine, then spreading the crushed grass at a ventilated place, and airing the moisture on the surface layer until the grass is not agglomerated;
weighing the rest padding materials: weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 30% of grass, 56% of sawdust, 7% of wheat bran and 7% of corn flour;
uniformly mixing all padding to prepare a fungus grass padding mixture: taking special bacterial liquid for the fermentation bed, and preparing the special bacterial liquid for the fermentation bed according to the following steps: diluting with sterile water at a volume ratio of 1:45, slowly adding the diluted special bacterial liquid for the fermentation bed into the mixed padding, continuously stirring the mixture without agglomerating the mixture, and adjusting the mixture until the water content of the mixture is 45% to obtain a grass and fungus padding mixture;
preparing a finished product of the fungus grass fermentation bed: stacking the fungus grass padding mixture into a cone, randomly inserting 15 holes with the aperture of 6cm and the depth of 20cm into the side surface of the cone, covering a breathable film, and stacking and fermenting for 6 days at the temperature of 25 ℃ to obtain a finished fungus grass fermentation bed;
and (3) transferring the finished product of the fungus grass fermentation bed into a pheasant breeding house, and paving the pheasant breeding house on the ground of the breeding house, wherein the thickness of the pheasant breeding bed is 30cm, so that the fungus grass fermentation bed is obtained.
Example 2
Preparing special bacterial liquid for a fermentation bed: respectively weighing 6% of EM (effective microorganism) bacterium powder, 11% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterium powder, the brown sugar powder and the sterile water is 100%, putting the brown sugar powder into a beaker, adding a small amount of hot water for dissolving, adding 80% of sterile water after dissolving, uniformly stirring and cooling, putting the EM bacterium powder into another beaker, adding 5% of sterile water for dissolving, pouring the dissolved brown sugar water and bacterium liquid into a fermentation bottle, adding the balance of sterile water, and then putting the fermentation bottle in a room for fermentation for 3 days to obtain the bacterium liquid special for the fermentation bed;
preparing a fungus grass padding: selecting fresh grass, crushing the fresh grass into filaments with the length of about 3cm by using a thread rolling machine, then spreading the crushed grass at a ventilated place, and airing the moisture on the surface layer until the grass is not agglomerated;
weighing the rest padding materials: weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 10% of grass, 72% of sawdust, 9% of wheat bran and 9% of corn meal;
uniformly mixing all padding to prepare a fungus grass padding mixture: taking special bacterial liquid for the fermentation bed, and preparing the special bacterial liquid for the fermentation bed according to the following steps: diluting with sterile water at a volume ratio of 1:50, slowly adding the diluted special bacterial liquid for the fermentation bed into the mixed padding, continuously stirring the mixture without agglomerating the mixture, and adjusting the mixture until the water content of the mixture is 40% to obtain a grass and fungus padding mixture;
preparing a finished product of the fungus grass fermentation bed: stacking the fungus grass padding mixture into a cone, randomly inserting 10 holes with the aperture of 4cm and the depth of 20cm into the side surface of the cone, covering a breathable film, and stacking and fermenting for 5 days in an environment of 23 ℃ to obtain a fungus grass fermentation bed finished product;
and (4) transferring the finished product of the fungus grass fermentation bed into a pheasant breeding house, and paving the pheasant breeding house on the ground of the breeding house, wherein the thickness of the pheasant breeding house is 25cm, so that the fungus grass fermentation bed is obtained.
Example 3
Preparing special bacterial liquid for a fermentation bed: respectively weighing 6% of EM (effective microorganism) bacterium powder, 11% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterium powder, the brown sugar powder and the sterile water is 100%, putting the brown sugar powder into a beaker, adding a small amount of hot water for dissolving, adding 85% of sterile water after dissolving, and cooling after uniformly stirring; putting EM (effective microorganism) powder into another beaker, adding 10% of sterile water for dissolving, pouring the dissolved brown sugar water and the bacteria liquid into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade for fermentation for 5 days to obtain the bacteria liquid special for the fermentation bed;
preparing a fungus grass padding: selecting fresh grass, crushing the fresh grass into filaments with the length of about 5cm by using a thread rolling machine, spreading the crushed grass at a ventilated place, and airing the moisture on the surface layer until the grass is not agglomerated;
weighing the rest padding materials: weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 50% of fungus grass, 40% of sawdust, 5% of wheat bran and 5% of corn flour;
uniformly mixing all padding to prepare a fungus grass padding mixture: taking special bacterial liquid for the fermentation bed, and preparing the special bacterial liquid for the fermentation bed according to the following steps: diluting with sterile water at a volume ratio of 1:40, slowly adding the diluted special bacterial liquid for the fermentation bed into the mixed padding, continuously stirring the mixture without agglomerating the mixture, and adjusting the mixture until the water content of the mixture is 50% to obtain a grass and fungus padding mixture;
preparing a finished product of the fungus grass fermentation bed: stacking the fungus grass padding mixture into a cone, randomly inserting 15 holes with the aperture of 6cm and the depth of 15cm into the side surface of the cone, covering a breathable film, and stacking and fermenting for 7 days at the temperature of 27 ℃ to obtain a finished fungus grass fermentation bed;
and (4) transferring the finished product of the fungus grass fermentation bed into a colorful pheasant breeding house, and paving the product on the ground of the breeding house with the thickness of 35cm to obtain the fungus grass fermentation bed.
Example 4
Preparing special bacterial liquid for a fermentation bed: respectively weighing 5.5% of EM (effective microorganism) bacterium powder, 11.5% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterium powder, the brown sugar powder and the sterile water is 100%, putting the brown sugar powder into a beaker, adding a small amount of hot water for dissolving, adding 81% of sterile water after dissolving, and cooling after uniformly stirring; putting EM (effective microorganism) powder into another beaker, adding 6% of sterile water for dissolving, pouring the dissolved brown sugar water and the bacteria liquid into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade for fermentation for 3 days to obtain the bacteria liquid special for the fermentation bed;
preparing a fungus grass padding: selecting fresh grass, crushing the fresh grass into filaments with the length of about 3cm by using a thread rolling machine, then spreading the crushed grass at a ventilated place, and airing the moisture on the surface layer until the grass is not agglomerated;
weighing the rest padding materials: weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 20% of grass, 64% of sawdust, 8% of wheat bran and 8% of corn meal;
uniformly mixing all padding to prepare a fungus grass padding mixture: taking special bacterial liquid for the fermentation bed, and preparing the special bacterial liquid for the fermentation bed according to the following steps: diluting with sterile water at a volume ratio of 1:42, slowly adding the diluted special bacterial liquid for the fermentation bed into the mixed padding, continuously stirring the mixture without agglomerating the mixture, and adjusting the mixture until the water content of the mixture is 42% to obtain a grass and fungus padding mixture;
preparing a finished product of the fungus grass fermentation bed: stacking the fungus grass padding mixture into a cone, randomly inserting 12 holes with the aperture of 4cm and the depth of 20cm into the side surface of the cone, covering a breathable film, and stacking and fermenting for 6 days at the temperature of 24 ℃ to obtain a finished fungus grass fermentation bed;
and (4) transferring the finished product of the fungus grass fermentation bed into a pheasant breeding house, and paving the pheasant breeding house on the ground of the breeding house, wherein the thickness of the pheasant breeding bed is 28cm, so that the fungus grass fermentation bed is obtained.
Example 5
Preparing special bacterial liquid for a fermentation bed: respectively weighing 6% of EM (effective microorganism) bacterium powder, 12% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterium powder, the brown sugar powder and the sterile water is 100%, putting the brown sugar powder into a beaker, adding a small amount of hot water for dissolving, adding 84% of sterile water after dissolving, and cooling after uniformly stirring; putting EM (effective microorganism) powder into another beaker, adding 9% of sterile water for dissolving, pouring the dissolved brown sugar water and the bacteria liquid into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade for fermentation for 5 days to obtain the bacteria liquid special for the fermentation bed;
preparing a fungus grass padding: selecting fresh grass, crushing the fresh grass into filaments with the length of about 5cm by using a thread rolling machine, spreading the crushed grass at a ventilated place, and airing the moisture on the surface layer until the grass is not agglomerated;
weighing the rest padding materials: weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 40% of grass, 48% of sawdust, 6% of wheat bran and 6% of corn meal;
uniformly mixing all padding to prepare a fungus grass padding mixture: taking special bacterial liquid for the fermentation bed, and preparing the special bacterial liquid for the fermentation bed according to the following steps: diluting with sterile water at a volume ratio of 1:48, slowly adding the diluted special bacterial liquid for the fermentation bed into the mixed padding, continuously stirring the mixture without agglomerating the mixture, and adjusting the mixture until the water content of the mixture is 48% to obtain a grass and fungus padding mixture;
preparing a finished product of the fungus grass fermentation bed: stacking the fungus grass padding mixture into a cone, randomly inserting 14 holes with the aperture of 6cm and the depth of 18cm into the side surface of the cone, covering a breathable film, and stacking and fermenting for 7 days at the temperature of 26 ℃ to obtain a finished fungus grass fermentation bed;
and (4) transferring the finished product of the fungus grass fermentation bed into a pheasant breeding house, and paving the pheasant breeding house on the ground of the breeding house, wherein the thickness of the pheasant breeding bed is 34cm, so that the fungus grass fermentation bed is obtained.
The data of the fungus grass fermentation bed prepared by the preparation method of the fungus grass fermentation bed is compared with the data of the traditional fermentation bed as follows:
1. the overall difference pair ratio of each group of fermentation beds is shown in table 1:
Figure BDA0001635845250000121
table 1: comparison of overall differences among fermentation beds in each group
According to the comparison of the overall differences of the fermentation beds in the table 1, the organic matter content of the fermentation bed of 30% of the grass groups is slightly lower than that of the traditional group but higher than that of the other two grass groups, which indicates that the organic matter nutrient content of the fermentation bed of 30% of the grass groups is equivalent to that of the traditional fermentation bed, and the organic matter of the fermentation bed of 30% of the grass groups is maintained more durably according to the later fermentation effect; the heavy metal content of the fungus grass group is higher than that of the traditional group, but the content of the fungus grass group is within the allowable range from the fermentation effect and the culture effect of the fermentation bed, so that the fermentation and the culture are not influenced; from the total nitrogen content, the total nitrogen content of the fungus grass group fermentation bed is higher than that of the traditional group, the total nitrogen content of 30% of the fungus grass group is 2 times of that of the traditional group, a sufficient nitrogen source is provided for the growth and the propagation of beneficial strains, and the nitrogen loss of the 30% fungus grass group fermentation bed is the lowest through later-stage cultivation tests, and the nitrogen loss can be increased due to the excessive fungus grass replacement amount; the bedding cost can be greatly reduced by partial replacement of the fungi and grass.
2. The temperature change at the early stage of stacking fermentation is shown in table 2:
1d 2d 3d 4d 5d 6d 7d
legacy group 28℃ 47℃ 71℃ 69℃ 54℃ 44℃ 45℃
10% of Juncao group 27℃ 45℃ 68℃ 67℃ 52℃ 43℃ 42℃
30% of Juncao group 27℃ 44 70℃ 71℃ 56℃ 46℃ 45℃
50% of Juncao group 26℃ 46℃ 69℃ 68℃ 53 40℃ 39℃
Table 2: temperature change at earlier stage of accumulation fermentation
According to the results in table 2, the early fermentation temperatures of the grass and fungus groups and the traditional group are increased and then decreased, the highest temperature is over 60 ℃, the bedding materials are all in a fermentation state, and the temperature difference between the 30% of the grass and fungus groups and the traditional group in the temperature rising period and the temperature lowering period is small, so that the early fermentation temperature of the 30% of the grass and fungus groups is closer to that of the traditional group.
3. The change in litter temperature during fermentation bed cultivation is shown in table 3:
Figure BDA0001635845250000141
table 3: temperature change of padding during fermentation bed cultivation
As can be seen from the core temperature change analysis of the central layer (20cm) of the padding, the first 20d of the grass group and the traditional group both belong to a temperature rise period, the central temperature reaches the highest value in the 20d, and then the temperature starts to decrease, so that the temperature rise speed of 30% of the grass groups is the fastest, and the temperature rise amplitude is the largest; the temperature of each group is in a descending trend from 20d to 40d, wherein the temperature of 10 percent of the grass groups and 30 percent of the grass groups is basically equal to that of the traditional group; 60d, the traditional group has a significant difference compared with 3 grass groups, the temperature of 10 percent grass groups and 50 percent grass groups is lower than that of the traditional group, while the temperature of 30 percent grass groups is higher than that of the traditional group, the significant difference is that due to the fact that a certain proportion (30 percent) of grass replaces sawdust and is beneficial to microbial activity after being mixed with livestock manure, heat is continuously generated, the pore space of a fermentation bed padding is increased due to the excessive grass replacing proportion, heat generated by microbial activity can be dissipated along with air flow and livestock activity, and the heat preservation effect of the fermentation bed is reduced.
4. The temperature change curve of the normal fermentation bed in the padding fermentation ripening process in the early 22 days of winter and summer is shown in figure 1: the temperature change of the normal fermentation bed is obvious in the early stage of fermentation, the temperature is raised to the highest temperature in about 5d, then the temperature is gradually reduced, and finally the temperature is maintained at about 40 ℃.
5. The change of water content during the cultivation in the fermentation bed is shown in Table 4,
day 0 Day 10 Day 20 Day 40 Day 60
Legacy group 47.31% 44.42% 42.66% 35.57% 30.82%
10% of Juncao 48.87% 45.12% 40.02% 36.75% 32.37%
30% of Juncao 47.62% 45.34% 41.99% 32.13% 30.96%
50% of Juncao 48.54% 40.64% 35.11% 30.79% 21.77%
Table 4: water content change during fermentation bed cultivation
The moisture content of each treatment group was maintained between 20% and 50% over the test period of 60 days, with an overall decreasing trend. In the traditional group, the water content is reduced by 16.49% in a 60-day test period; the water content of 10 percent of the fungus grass is reduced by 16.5 percent in a 60-day test period; the water content of 30 percent of the fungus grass groups is reduced by 16.66 percent in a 60-day test period; the water content of the 50% Juncao + 50% control group decreased by 26.77% in the 60-day test period. The temperature rises sharply during fermentation to cause the moisture content to drop, and the moisture attached to the film is absorbed by the padding again along with the temperature reduction, so that the moisture content slowly rises, the fermentation is started up at an accelerated speed along with the initial addition of the livestock and poultry manure from day 0 to day 10, the lost moisture content is balanced with the addition of the chicken manure, and the moisture content is not changed greatly. After 20 days, the fermentation bed works stably, and the activities of a large proportion of the fungus grass treatment groups and the livestock and poultry are key factors of the change of the water content. On the 60 th day, the water content of 10% of the fungus grass group and 30% of the fungus grass group is still kept above 30%, the water content is similar to that of the traditional group, and the water content of 50% of the fungus grass group is the lowest. The result shows that the addition of a certain proportion (30%) of the grass has better water retention performance, and the excessive proportion of the grass can accelerate the water loss of the fermentation bed padding.
6. The toxicity of each set of fermentation bed padding on plants is tested, the results are shown in figures 2-6, the toxicity test data are shown in table 5, and the results are summarized as follows:
planting rape seeds in the distilled water of the control group, sowing 60 seeds and germinating 16 seeds, wherein the longest root length is 75.79 mm;
traditionally, 60 seeds are sowed in a group to germinate 4 seeds, and the longest root length is 109.36 mm;
sowing 60 fungi grass seeds with 10 percent of the fungi grass group to sprout 6 fungi grass seeds, wherein the longest root length is 61.43 mm;
60 bacteria and grass are sowed in 30 percent of the fungus and grass groups to sprout 14 bacteria and grass, and the longest root length is 118.59 mm;
60 seeds of 50 percent of the fungus grass are sown in a group to sprout 6 seeds, and the longest root length is 5.86 mm.
Figure BDA0001635845250000161
Figure BDA0001635845250000171
Table 5: toxicity test data sheet
The toxicity of the 30% grass group to the seeds is the minimum, which shows that the 30% grass fermentation bed can be used as planting fertilizer after the use, and the subsequent utilization value is exerted.
From the above summary data it is shown that: the fermentation temperature change of the 30% fungus grass group fermentation bed padding follows a padding fermentation ripening process curve; the temperature change of the padding during the culture process shows that the temperature holding effect of 30 percent of the fungus grass groups is the best, which is beneficial to the mass propagation of beneficial microorganism strains and accelerates the speed of decomposing the livestock and poultry manure; the water holding performance of the 30% fungus grass group is optimal and slightly higher than that of the traditional group according to the change of the water content of the padding in the culture process, and the nutritional ingredients of the padding are not easy to volatilize along with the air flow and the activities of livestock and poultry; as can be seen from the toxicity test results, the subsequent utilization value of the 30% grass group fermentation bed padding is high, the toxicity to plants is minimum, the nutrient content is highest, the germination rate of the plants is highest, and the roots are longer when the padding is used for planting the plants. Therefore, the series of experiments show that the fermentation bed of 30% of the grass and fungus groups has the best comprehensive effect.

Claims (1)

1. The preparation method of the fungus grass fermentation bed is characterized by comprising the following steps:
step 1, preparing special bacteria liquid for fermentation bed
Step 1.1, respectively weighing 5-6% of EM (effective microorganism) bacterial powder, 11-12% of brown sugar powder and the balance of sterile water according to the mass percentage, wherein the sum of the mass percentages of the EM bacterial powder, the brown sugar powder and the sterile water is 100%;
step 1.2, putting the brown sugar powder weighed in the step 1.1 into a beaker, adding a small amount of hot water for dissolving, adding 80-85% of sterile water after dissolving, stirring uniformly and cooling;
step 1.3, putting the EM bacterial powder measured in the step 1.1 into another beaker, and adding 5-10% of sterile water for dissolving;
step 1.4, pouring the brown sugar dissolved in the step 1.2 and the bacterial liquid dissolved in the step 1.3 into a fermentation bottle, adding the rest sterile water, and then placing the fermentation bottle in an indoor shade for fermentation for 3-5 days to obtain the bacterial liquid special for the fermentation bed;
step 2, preparing the fungus grass padding
Selecting fresh grass, crushing the fresh grass into a filament shape with the length of about 3-5 cm by using a thread rolling machine, spreading the crushed grass at a ventilated place, and airing the moisture on the surface until the grass is not agglomerated;
step 3, weighing the rest padding materials
Weighing various padding materials of the fungus grass fermentation bed according to the mass percentage: 10-50% of grass, 40-72% of sawdust, 5-9% of wheat bran and 5-9% of corn flour, wherein the sum of the mass percentages of the padding is 100%;
step 4, uniformly mixing all padding to prepare a fungus grass padding mixture
Step 4.1, taking the special bacterial liquid for the fermentation bed prepared in the step 1, and carrying out fermentation according to the special bacterial liquid for the fermentation bed: diluting with sterile water in a volume ratio of 1: 50-1: 40;
step 4.2, adding the bacterial liquid special for the fermentation bed diluted in the step 4.1 into the mixed padding weighed in the step 3, and adjusting the water content of the padding mixture to 40-50% to obtain a grass-fungus padding mixture;
step 4.2.1, uniformly mixing the sawdust and the fungus grass weighed in the step 3;
step 4.2.2, adding the wheat bran and the corn flour weighed in the step 3, and uniformly mixing to prepare a mixed padding;
step 4.2.3, slowly adding the special bacterial liquid for the fermentation bed diluted in the step 4.1 into the mixed padding prepared in the step 4.2.2, stirring the mixture continuously without stopping stirring the mixture, and adjusting the water content of the mixture to be 40-50%;
step 5, preparing a finished product of the fungus grass fermentation bed
Stacking the fungus and grass padding mixture prepared in the step (4) into a cone, randomly inserting 10-15 holes with the aperture of 4-6 cm and the depth of 15-20 cm into the side surface of the cone-shaped stacking object, covering a breathable film, stacking and fermenting for 5-7 days at the stacking and fermenting temperature of 23-27 ℃, and obtaining a fungus and grass fermentation bed finished product;
and 6, transferring the finished product of the fungus grass fermentation bed obtained in the step 5 into a pheasant breeding house, flatly paving the product on the ground of the breeding house, and paving the product with the thickness of 25-35 cm to obtain the fungus grass fermentation bed.
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