CN107365725B - A kind of organic matter decomposing inoculant and preparation method thereof - Google Patents
A kind of organic matter decomposing inoculant and preparation method thereof Download PDFInfo
- Publication number
- CN107365725B CN107365725B CN201710648274.5A CN201710648274A CN107365725B CN 107365725 B CN107365725 B CN 107365725B CN 201710648274 A CN201710648274 A CN 201710648274A CN 107365725 B CN107365725 B CN 107365725B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- preparation
- parts
- aspergillus oryzae
- seed liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 101
- 239000002054 inoculum Substances 0.000 title claims abstract description 83
- 239000005416 organic matter Substances 0.000 title claims abstract description 49
- 238000000855 fermentation Methods 0.000 claims abstract description 208
- 230000004151 fermentation Effects 0.000 claims abstract description 208
- 239000007788 liquid Substances 0.000 claims abstract description 81
- 239000007787 solid Substances 0.000 claims abstract description 78
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 62
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 62
- 241000894006 Bacteria Species 0.000 claims abstract description 56
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 48
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 42
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 42
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 40
- 241000186046 Actinomyces Species 0.000 claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 28
- 241000194017 Streptococcus Species 0.000 claims abstract description 25
- 239000004310 lactic acid Substances 0.000 claims abstract description 20
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 20
- 241000195940 Bryophyta Species 0.000 claims abstract description 13
- 239000012467 final product Substances 0.000 claims abstract description 6
- 239000002609 medium Substances 0.000 claims description 73
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 70
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 42
- 235000015097 nutrients Nutrition 0.000 claims description 36
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 35
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 35
- 239000001963 growth medium Substances 0.000 claims description 34
- 239000012530 fluid Substances 0.000 claims description 33
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 30
- 239000001888 Peptone Substances 0.000 claims description 30
- 108010080698 Peptones Proteins 0.000 claims description 30
- 239000008103 glucose Substances 0.000 claims description 30
- 235000019319 peptone Nutrition 0.000 claims description 30
- 230000003519 ventilatory effect Effects 0.000 claims description 25
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 24
- 239000011780 sodium chloride Substances 0.000 claims description 21
- 229940041514 candida albicans extract Drugs 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 20
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 20
- 239000012138 yeast extract Substances 0.000 claims description 20
- 239000000203 mixture Substances 0.000 claims description 19
- 210000003608 fece Anatomy 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 16
- 239000010871 livestock manure Substances 0.000 claims description 15
- 241000287828 Gallus gallus Species 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 235000005822 corn Nutrition 0.000 claims description 13
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- 239000004615 ingredient Substances 0.000 claims description 10
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 10
- 229920000053 polysorbate 80 Polymers 0.000 claims description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 10
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 9
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000013312 flour Nutrition 0.000 claims description 5
- 229940099596 manganese sulfate Drugs 0.000 claims description 5
- 239000011702 manganese sulphate Substances 0.000 claims description 5
- 235000007079 manganese sulphate Nutrition 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- 239000004323 potassium nitrate Substances 0.000 claims description 5
- 235000010333 potassium nitrate Nutrition 0.000 claims description 5
- 239000001632 sodium acetate Substances 0.000 claims description 5
- 235000017281 sodium acetate Nutrition 0.000 claims description 5
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 5
- 239000001393 triammonium citrate Substances 0.000 claims description 5
- 235000011046 triammonium citrate Nutrition 0.000 claims description 5
- 239000012137 tryptone Substances 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 241000186361 Actinobacteria <class> Species 0.000 claims description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 2
- 239000005864 Sulphur Substances 0.000 claims description 2
- 238000007790 scraping Methods 0.000 claims 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 240000003768 Solanum lycopersicum Species 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 229910052742 iron Inorganic materials 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 13
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 7
- 239000003795 chemical substances by application Substances 0.000 abstract description 7
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 7
- 239000011574 phosphorus Substances 0.000 abstract description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 6
- 239000011591 potassium Substances 0.000 abstract description 6
- 229910052700 potassium Inorganic materials 0.000 abstract description 6
- 239000011368 organic material Substances 0.000 abstract description 5
- 238000010792 warming Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008635 plant growth Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 32
- 238000009630 liquid culture Methods 0.000 description 12
- 241000209094 Oryza Species 0.000 description 9
- 235000007164 Oryza sativa Nutrition 0.000 description 9
- 239000010903 husk Substances 0.000 description 9
- 235000009566 rice Nutrition 0.000 description 9
- 241000209140 Triticum Species 0.000 description 8
- 235000021307 Triticum Nutrition 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 239000003337 fertilizer Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 238000009264 composting Methods 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 244000144977 poultry Species 0.000 description 5
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- 241000227653 Lycopersicon Species 0.000 description 4
- 239000000460 chlorine Substances 0.000 description 4
- 229910052801 chlorine Inorganic materials 0.000 description 4
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000000243 photosynthetic effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- GMKMEZVLHJARHF-UHFFFAOYSA-N (2R,6R)-form-2.6-Diaminoheptanedioic acid Natural products OC(=O)C(N)CCCC(N)C(O)=O GMKMEZVLHJARHF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- GMKMEZVLHJARHF-SYDPRGILSA-N meso-2,6-diaminopimelic acid Chemical compound [O-]C(=O)[C@@H]([NH3+])CCC[C@@H]([NH3+])C([O-])=O GMKMEZVLHJARHF-SYDPRGILSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of organic matter decomposing inoculant and preparation method thereof, decomposing agent includes bacillus subtilis, actinomyces, lactic acid bacteria, aspergillus oryzae and saccharomycete；Further, organic matter decomposing inoculant includes 5-10 parts of bacillus subtilis fermentation liquor, 40-45 parts of unwrapping wire bacteria solid fermentation object, 5-10 parts of streptococcus acidi lactici fermented solution, 30-40 parts of Aspergillus oryzae solid culture, 5-10 parts of saccharomycetes to make fermentation liquid, 0-10 parts of rotten mosses ash.Preparation method: bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, Aspergillus oryzae solid culture, saccharomycetes to make fermentation liquid and rotten mosses ash are mixed in proportion to obtain the final product.Organic matter decomposing inoculant of the invention can make organic materials be warming up to 60 degree or more at 2-3 days, and continue 5-7 days at such a temperature；The present invention can by material slightly solubility potassium and phosphorus release to accelerate plant growth, improve soil environment；Dosage of the present invention is few, and effect is good.
Description
Technical field
The present invention relates to a kind of organic matter decomposing inoculants and preparation method thereof, belong to technical field of soil fertilizer.
Background technique
Composting process is to promote process of the biodegradable organic to stable humus biochemical conversion using microorganism,
Compost is divided into 4 step-up temperatures, high temperature, cooling and decomposed.With the development of livestock and poultry breeding industry, feces of livestock and poultry causes sternly environment
The pollution of weight；As the preferable organic fertilizer of fertilizer efficiency, but wherein, nutrient exists poultry manure mostly in the form of organic compound, difficult
It is utilized with being absorbed by crops.Meanwhile field crops stalk, often through burning disposal, burning not only wastes resource and pollution
Environment.
Bacillus subtilis is one kind of bacillus, is aerobic, bacillus, the bacterium is as plant disease biological and ecological methods to prevent plant disease, pests, and erosion
One of bacterium has stronger prophylaxis effect, in extreme conditions, can also induce and generate the very strong gemma of resistance, should
Bacterium is readily produced, and carries out formulation, and be easy to survive, colonizes and breed.
Actinomyces thallus be it is unicellular, be made of mostly the mycelia of branch prosperity, it is simplest for it is rod-shaped or have original bacteria
Silk.Hyphal diameter is similar with bacillus, and about 1 micron.Also containing born of the same parents specific to prokaryotes in cell wall chemical composition
Teichaic acid and diaminopimelic acid are free of chitin or cellulose；Its characteristic most outstanding first is that can generate that a large amount of, type is numerous
More antibiotic.
Lactic acid bacteria refers to that fermenting carbohydrate primary product is a kind of general name without gemma, gram-positive bacterium of lactic acid；
Lactic acid bacteria can pass through the substances such as the organic acid of fermentation generation, special enzyme system, sour rhzomorph.
Aspergillus oryzae can intensive decomposition protein, cellulose, hemicellulose, lignin etc., and by thermophilic, thermoduric bacteria,
Fungi, yeast strain and correlation down enzyme are combined, and living bacteria count content is high, and degradation capability is strong, while can reach
To heating, deodorization, eliminate pest and disease damage, weed seed and the effect for improving nutrient.It under appropriate conditions, can rapidly will be in windrow
Carbon, nitrogen, phosphorus, potassium, sulphur etc. decompose mineralising, form simple organic.
Saccharomycete is some unicellular fungis, not the unit of phylogeny classification.A kind of invisible small list
Sugar can be fermented into alcohol and carbon dioxide, be distributed in entire nature by cell microorganism, be that a kind of typical amphimicrobian is micro-
Biology can survive under aerobic and oxygen free condition, be natural fermented dose a kind of.
Summary of the invention
The present invention provides a kind of organic matter decomposing inoculant and preparation method thereof, the present invention can make material (feces of livestock and poultry,
Mushroom residue, crop material, bean dregs etc.) it is brought rapidly up, 60 degree or more are warming up within 2-3 days, quick composting, 10 days or so are decomposed complete
Entirely.Technical scheme is as follows:
A kind of organic matter decomposing inoculant, including following components:
Bacillus subtilis, actinomyces, lactic acid bacteria, aspergillus oryzae and saccharomycete.
Further, organic matter decomposing inoculant of the present invention, the component including following parts by weight:
5-10 parts of bacillus subtilis fermentation liquor, 40-45 parts of unwrapping wire bacteria solid fermentation object, 5-10 parts of streptococcus acidi lactici fermented solution,
30-40 parts of Aspergillus oryzae solid culture, 5-10 parts of saccharomycetes to make fermentation liquid, 0-10 parts of rotten mosses ash.
Further, organic matter decomposing inoculant of the present invention, the component including following parts by weight:
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation object, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
34 parts of fermentation material, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash.
In the present invention, organic matter decomposing inoculant the preparation method is as follows:
The first, the preparation of bacillus subtilis fermentation liquor:
(1) preparation of seed liquor: the thallus that one connects collarium bacillus subtilis is scraped from slant medium and is inoculated into liquid
In body culture medium, shaking table culture 12h, revolving speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L): tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is that 100-120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h,
Fermentation time is 18-20h to get bacillus subtilis fermentation liquor；Preferably, revolving speed is 110 turns/min, fermentation time 19h.
Fermentation medium (g/L): corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9, ammonium sulfate 1, K2HPO4
0.3, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
The second, the preparation of unwrapping wire bacteria solid fermentation object:
(1) preparation of seed liquor: the thallus that one connects collarium actinomyces is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L): potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of fermentation liquid:
By step (1) obtain seed liquor be inoculated into fermentor with the inoculum concentration of 10% (v/v), revolving speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentor, ventilatory capacity 6m3Ferment under conditions of/h, fermentation time be 30-32h to get
Actinomycetes fermentation liquor；Preferably, fermentation time 31h.
Actinomyces liquid fermentation medium (g/L): glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Pile；3. fermentation management: when temperature of charge no longer improves after heating up, it is solid to get actinomyces that turning continues culture 3-5 days
Body fermentation material；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent): chicken manure (dry weight) 50%, mushroom residue 50% mix.
The preparation of third, streptococcus acidi lactici fermented solution:
(1) preparation of seed liquor: the thallus that one connects collarium lactic acid bacteria is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L): peptone 10.0, powdered beef 5.0, yeast powder 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is that 100-120 turns/min in revolving speed, temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3Under conditions of/h, pH are 6-6.5
It ferments, fermentation time is 22-24h to get streptococcus acidi lactici fermented solution；Preferably, revolving speed is 110 turns/min, pH 6.2, hair
The ferment time is 23h.
Lactobacillus-fermented culture medium (g/L): peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture:
(1) preparation of seed liquor: the preparation of seed liquor: the thallus that one connects collarium aspergillus oryzae is scraped from slant medium and is connect
Kind is into fluid nutrient medium, shaking table culture 12h, revolving speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L): urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 28-30h to get aspergillus oryzae fermentation liquid；Preferably, fermentation time 29h.
Aspergillus oryzae fermentation medium (g/L): urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is connect with the inoculum concentration (m/m) of 10-15%
Kind is mixed into aspergillus oryzae solid fermentation culture medium；2. heap processed: length 10m, wide 2-4m, height 30- is made in the material of mixing
The pile of 40cm；3. fermentation management: for temperature of charge not when improving, turning continues culture 3-5 days to get meter Qu after heating up
Mould solid fermentation object；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor: the thallus that one connects collarium saccharomycete is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L): yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 12-14h to get saccharomycetes to make fermentation liquid；Preferably, fermentation time 13h.
Saccharomycetes to make fermentation culture medium (g/L): peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, adds water to 1L, pH 7.0-7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment object, saccharomycetes to make fermentation liquid and rotten mosses ash mix in proportion to obtain the final product.
Compared with the prior art, the present invention has the following advantages:
(1) organic matter decomposing inoculant of the invention can make organic materials be warming up to 60 degree or more at 2-3 days, and in the temperature
Degree is lower to continue 5-7 days；
(2) present invention can by material slightly solubility potassium and phosphorus release to accelerate plant growth, while the present invention
Addition has antagonistic strain that can inhibit the growth of pathogenic bacteria in soil, improves soil environment；
(3) dosage of the present invention is few, can quickly make organic material composting；
(4) present invention can not only carry out stalk decomposed, can also carry out to feces of livestock and poultry decomposed；
(5) straw-returning applies organic matter decomposing inoculant of the invention simultaneously can be improved yield, and improve soil knot
Structure.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.But examples are merely exemplary, and it is not intended to limit the scope of the present invention in any way.Those skilled in the art answer
It should be appreciated that without departing from the spirit and scope of the invention can details to technical solution of the present invention and form repair
Change or replace, but these modifications and replacement are fallen within the protection scope of the present invention.
Mushroom and reagent used are commercially available in the present invention.
A kind of organic matter decomposing inoculant of embodiment 1 and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight:
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation object, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
34 parts of fermentation material, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash；
Above-mentioned organic matter decomposing inoculant the preparation method is as follows:
The first, the preparation of bacillus subtilis fermentation liquor:
(1) preparation of seed liquor: the thallus that one connects collarium bacillus subtilis is scraped from slant medium and is inoculated into liquid
In body culture medium, shaking table culture 12h, revolving speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L): tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 110 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments, ferments under conditions of/h
Time is 19h to get bacillus subtilis fermentation liquor；
Fermentation medium (g/L): corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9, ammonium sulfate 1, K2HPO4
0.3, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
The second, the preparation of unwrapping wire bacteria solid fermentation object:
(1) preparation of seed liquor: the thallus that one connects collarium actinomyces is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L): potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of fermentation liquid:
By step (1) obtain seed liquor be inoculated into fermentor with the inoculum concentration of 10% (v/v), revolving speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is 31h to get putting
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L): glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Pile；3. fermentation management: when temperature of charge no longer improves after heating up, turning continues culture 4 days to get actinomyces solid
Fermentation material；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent): chicken manure (dry weight) 50%, mushroom residue 50% mix.
The preparation of third, streptococcus acidi lactici fermented solution:
(1) preparation of seed liquor: the thallus that one connects collarium lactic acid bacteria is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L): peptone 10.0, powdered beef 5.0, yeast powder 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 110 turns/min in revolving speed, temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3/ h, pH are sent out under conditions of being 6.2
Ferment, fermentation time are 23h to get streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L): peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture:
(1) preparation of seed liquor: the preparation of seed liquor: the thallus that one connects collarium aspergillus oryzae is scraped from slant medium and is connect
Kind is into fluid nutrient medium, shaking table culture 12h, revolving speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L): urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 29h to get aspergillus oryzae fermentation liquid；
Aspergillus oryzae fermentation medium (g/L): urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is connect with the inoculum concentration (m/m) of 10-15%
Kind is mixed into aspergillus oryzae solid fermentation culture medium；2. heap processed: length 10m, wide 2-4m, height 30- is made in the material of mixing
The pile of 40cm；3. fermentation management: for temperature of charge not when improving, turning continues culture 4 days to get aspergillus oryzae after heating up
Solid fermentation object；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor: the thallus that one connects collarium saccharomycete is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L): yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 13h to get saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L): peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, adds water to 1L, pH 7.0；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment object, saccharomycetes to make fermentation liquid and rotten mosses ash mix in proportion to obtain the final product.
A kind of organic matter decomposing inoculant of embodiment 2 and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight:
6 parts of bacillus subtilis fermentation liquor, 41 parts of unwrapping wire bacteria solid fermentation object, 7 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
38 parts of fermentation material, 9 parts of saccharomycetes to make fermentation liquid, 8 parts of rotten mosses ash；
Above-mentioned organic matter decomposing inoculant the preparation method is as follows:
The first, the preparation of bacillus subtilis fermentation liquor:
(1) preparation of seed liquor: the thallus that one connects collarium bacillus subtilis is scraped from slant medium and is inoculated into liquid
In body culture medium, shaking table culture 12h, revolving speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L): tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments, ferments under conditions of/h
Time is 20h to get bacillus subtilis fermentation liquor；
Fermentation medium (g/L): corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9, ammonium sulfate 1, K2HPO4
0.3, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
The second, the preparation of unwrapping wire bacteria solid fermentation object:
(1) preparation of seed liquor: the thallus that one connects collarium actinomyces is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L): potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of fermentation liquid:
By step (1) obtain seed liquor be inoculated into fermentor with the inoculum concentration of 10% (v/v), revolving speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is 32h to get putting
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L): glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Pile；3. fermentation management: when temperature of charge no longer improves after heating up, turning continues culture 5 days to get actinomyces solid
Fermentation material；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent): chicken manure (dry weight) 50%, mushroom residue 50% mix.
The preparation of third, streptococcus acidi lactici fermented solution:
(1) preparation of seed liquor: the thallus that one connects collarium lactic acid bacteria is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L): peptone 10.0, powdered beef 5.0, yeast powder 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3/ h, pH are sent out under conditions of being 6.1
Ferment, fermentation time are 22h to get streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L): peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture:
(1) preparation of seed liquor: the preparation of seed liquor: the thallus that one connects collarium aspergillus oryzae is scraped from slant medium and is connect
Kind is into fluid nutrient medium, shaking table culture 12h, revolving speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L): urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 30h to get aspergillus oryzae fermentation liquid；
Aspergillus oryzae fermentation medium (g/L): urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is connect with the inoculum concentration (m/m) of 10-15%
Kind is mixed into aspergillus oryzae solid fermentation culture medium；2. heap processed: length 10m, wide 2-4m, height 30- is made in the material of mixing
The pile of 40cm；3. fermentation management: for temperature of charge not when improving, turning continues culture 5 days to get aspergillus oryzae after heating up
Solid fermentation object；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor: the thallus that one connects collarium saccharomycete is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L): yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 12h to get saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L): peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, adds water to 1L, pH 7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment object, saccharomycetes to make fermentation liquid and rotten mosses ash mix in proportion to obtain the final product.
A kind of organic matter decomposing inoculant of embodiment 3 and preparation method thereof
Organic matter decomposing inoculant includes the component of following parts by weight:
8 parts of bacillus subtilis fermentation liquor, 44 parts of unwrapping wire bacteria solid fermentation object, 6 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
32 parts of fermentation material, 6 parts of saccharomycetes to make fermentation liquid；
Above-mentioned organic matter decomposing inoculant the preparation method is as follows:
The first, the preparation of bacillus subtilis fermentation liquor:
(1) preparation of seed liquor: the thallus that one connects collarium bacillus subtilis is scraped from slant medium and is inoculated into liquid
In body culture medium, shaking table culture 12h, revolving speed 200r/min, temperature are 30 DEG C, obtain the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L): tryptone 10, yeast extract 5, NaCl 10, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 100 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments, ferments under conditions of/h
Time is 18h to get bacillus subtilis fermentation liquor；
Fermentation medium (g/L): corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9, ammonium sulfate 1, K2HPO4
0.3, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
The second, the preparation of unwrapping wire bacteria solid fermentation object:
(1) preparation of seed liquor: the thallus that one connects collarium actinomyces is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L): potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5,
Ferrous sulfate heptahydrate 0.01, water complements to 1L；
(2) preparation of fermentation liquid:
By step (1) obtain seed liquor be inoculated into fermentor with the inoculum concentration of 10% (v/v), revolving speed be 120 turns/
Min, temperature are 28 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is 30h to get putting
Line fermented liquid；
Actinomyces liquid fermentation medium (g/L): glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2,
Dipotassium hydrogen phosphate 0.4, epsom salt 0.05, water complements to 1L.
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into 10% inoculum concentration (m/m)
In unwrapping wire bacteria solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Pile；3. fermentation management: when temperature of charge no longer improves after heating up, turning continues culture 4 days to get actinomyces solid
Fermentation material；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent): chicken manure (dry weight) 50%, mushroom residue 50% mix.
The preparation of third, streptococcus acidi lactici fermented solution:
(1) preparation of seed liquor: the thallus that one connects collarium lactic acid bacteria is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L): peptone 10.0, powdered beef 5.0, yeast powder 4.0, glucose 20.0, tween
80 1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to
1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 110 turns/min in revolving speed, temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3/ h, pH are sent out under conditions of being 6.5
Ferment, fermentation time are for 24 hours to get streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L): peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato
Powder 0.25, Tween 80 0.1 are leached, water complements to 1L.
4th, the preparation of Aspergillus oryzae solid culture:
(1) preparation of seed liquor: the preparation of seed liquor: the thallus that one connects collarium aspergillus oryzae is scraped from slant medium and is connect
Kind is into fluid nutrient medium, shaking table culture 12h, revolving speed 220r/min, and temperature is 28 DEG C, obtains the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L): urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05, potassium dihydrogen phosphate 0.18, water complement to 1L.
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 28h to get aspergillus oryzae fermentation liquid；
Aspergillus oryzae fermentation medium (g/L): urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride
0.5, ferric sulfate 0.5. water supplies 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is connect with the inoculum concentration (m/m) of 10-15%
Kind is mixed into aspergillus oryzae solid fermentation culture medium；2. heap processed: length 10m, wide 2-4m, height 30- is made in the material of mixing
The pile of 40cm；3. fermentation management: for temperature of charge not when improving, turning continues culture 3 days to get aspergillus oryzae after heating up
Solid fermentation object；
Aspergillus oryzae solid fermentation culture medium is mushroom residue.
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor: the thallus that one connects collarium saccharomycete is scraped from slant medium and is inoculated into Liquid Culture
In base, shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L): yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) seed liquor that step (1) obtains the preparation of fermentation liquid: is inoculated into fermentor with the inoculum concentration of 10% (v/v)
In, it is 120 turns/min in revolving speed, temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, when fermentation
Between for 12h to get saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L): peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, chlorine
Change sodium 5, adds water to 1L, pH 7.0-7.2；
6th, bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, aspergillus oryzae solid are sent out
Ferment object, saccharomycetes to make fermentation liquid and rotten mosses ash mix in proportion to obtain the final product.
The manufacturer of commercially available organic matter decomposing inoculant is Beijing photosynthetic organism Science and Technology Ltd. in test example.
Application test of the organic matter decomposing inoculant of the present invention of test example 1 in wheat stalk returning
One, material and method
1, test site: experimental field it is located at Linyi Tancheng County；
2, test period: experiment was on July 1st, 2016；
3, test specimen: organic matter decomposing inoculant prepared by the embodiment of the present invention 1,2 and 3；
4, test method: being experimental field selected in Linyi Tancheng, and experimental field upper one batch of crop is wheat, tests ground
4 mu of product, is harvested on November 3rd, 2016 with harvester, and wheat height stays in 5 centimetres or less when harvesting；
Test is divided into three processing groups: test group: the organic material composting of stalk and the preparation of the embodiment of the present invention 1,2 and 3
Agent；Control group: stalk and equivalent clear water；
Test group: according to the of the present invention organic material composting of July 4 day per acre using 2kg of the test requirements document after completing stalk
50kg fine earth is mixed in agent, is uniformly spread fertilizer over the fields on stalk, and it is made to fully absorb moisture；
Control group: only spray equivalent clear water allows stalk to fully absorb moisture；
Field management: from decomposing agent on July 4 apply it is lower after, often to field observation and water is replenished in time, keeps stalk abundant
It keeps wet, does ridge between test group and control group to keep apart, it is made not to be interconnected.
Observation item or index: 1, each 10 day decomposed situation is observed；2, record start decomposed time and it is completely decomposed when
Between；
Two, test result
1, test group blackening since the 7th day gently starts to be broken with hand once drawing, and especially eustipes part is easily broken off, straw
Stalk is just divided into several sections, the complete blackening at the 14th day, and fiber rots substantially thoroughly, the control without using decomposing agent of the present invention
Group, in the basic still yellow of the 10th day observation color, tenacity of fibre is very good, and two hands can't be broken with stem pulling section more energetically,
Just there is fragmentary blackening to observation in the 20th day, fiber starts to decay, and can be broken with stipes when pulling, but color mass-tone is still with yellowish-brown
Based on, to being August 20th when complete blackening；As shown in table 1；Straw-returning can be shown with applying organic matter decomposing inoculant of the present invention
Write improve the soil organism, alkali-hydrolyzable nitrogen, available potassium and available phosphorus content (p < 0.05).Compared with the control group of the present invention, speed
The content for imitating potassium and available phosphorus is as shown in table 2.
The available potassium of test group increases 79% compared with the control group, and available phosphorus content improves compared with the control group
31%.
The decomposed record of table 1
2 soil available nitrogen of table and available phosphorus detection
The detection of 2 pairs of organic matter decomposing inoculants of the present invention of test example
One, material and method
1, test site: Liaocheng of Shandong Province Yanggu Xiang Yu organic fertilizer Co., Ltd；
2, test period: experiment was on August 1st, 2016；
3, test specimen: commercially available organic matter decomposing inoculant (Beijing photosynthetic organism Science and Technology Ltd.) and the embodiment of the present invention
1, organic matter decomposing inoculant prepared by 2 and 3；
4, test method:
Test group 1: 1kg is added in every cube of rice husk chicken manure in organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 1
Embodiment 1 prepare organic matter decomposing inoculant；
Test group 2: 1kg is added in every cube of rice husk chicken manure in organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 2
Embodiment 2 prepare organic matter decomposing inoculant；
Test group 3: 1kg is added in every cube of rice husk chicken manure in organic matter decomposing inoculant prepared by rice husk chicken manure and embodiment 2
Embodiment 3 prepare organic matter decomposing inoculant；
Control group: the commercially available organic of 2kg is added in every cube of rice husk chicken manure for rice husk chicken manure and commercially available organic matter decomposing inoculant
Material decomposing agent (m3/2KG)；
It all keeps the skin wet to 55% or so (being held with hand one can be discharged and not fall) simultaneously, every side's rice husk chicken manure adds 4kg to urinate
Element.Turning is primary every three days and keeps the skin wet according to the actual situation.
Observation item or index: 1, decomposed condition monitoring temperature is daily observed；2, decomposed completely rear survey germination percentage.
Two, test result
60 degree can be warming up within organic matter decomposing inoculant the 2nd day prepared by the embodiment of the present invention 1,2 and 3, corruption in 16 days
Ripe completion carries out germination percentage test with corn, and germination percentage reaches 90%, is warming up to 60 degree within commercially available organic matter decomposing inoculant the 4th day,
Germination percentage test is carried out with corn, germination percentage reaches 80%.
Influence of the organic matter decomposing inoculant of the invention of test example 3 to crop yield and soil texture
In order to preferably verify the effectiveness of organic matter decomposing inoculant of the present invention, decomposing agent is made using the embodiment of the present invention 1
Carry out crop planting test.
1. test material and method
(1) test period and place
Test site: the experimental plot Tai'an Shandong NongDa Fertilizer Science Co., Ltd Xing Runyuan.
Test period: -2016 years 2014.
Crop: wheat, corn
(2) experimental design
Test sets 2 processing, and upper one batch of stalk is with site preparation returning to the field.Processing 1 is control, only with conventional fertilizer application, place
+ 50% conventional fertilizer application of organic matter decomposing inoculant made from the embodiment 1 of reason 2, calculates crops annual output, as shown in Table 3: applying
After organic matter decomposing inoculant made from embodiment 1, significant changes are had occurred in corn and wheat yield, and straw-returning adds organic matter
Expect that First Year, corn and wheat yield are significantly increased, it is horizontal to basically reach middle production；Second year corn and wheat yield persistently increase
Long, especially it is horizontal to basically reach middle high yield for corn yield.It was tested by 2 years, compared with conventional fertilizer application, obvious effect of increasing production.
Influence of 3 different disposal of table to corn wheat yield
As shown in Table 4: made from embodiment 1 after organic matter decomposing inoculant continuous administration 2 years, compared with the control, significantly
The soil weight is reduced, improves soil powder and soil capillary porosity percentage, and then improve the water conservation of soil
Nutrient preserving capability and aeration, make soil physical property be improved.
The influence of 4 different disposal soil mechanical composition of table
Claims (8)
1. a kind of organic matter decomposing inoculant, which is characterized in that organic matter decomposing inoculant is composed of the following parts by weight: withered
Careless fermentation of bacillus liquid 5-10 parts, 40-45 parts of unwrapping wire bacteria solid fermentation object, 5-10 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid
30-40 parts of fermentation material, 5-10 parts of saccharomycetes to make fermentation liquid, 0-10 parts of rotten mosses ash.
2. organic matter decomposing inoculant according to claim 1, which is characterized in that organic matter decomposing inoculant is by following parts by weight
Several groups are grouped as:
7 parts of bacillus subtilis fermentation liquor, 42 parts of unwrapping wire bacteria solid fermentation object, 9 parts of streptococcus acidi lactici fermented solution, aspergillus oryzae solid fermentation
34 parts of object, 6 parts of saccharomycetes to make fermentation liquid, 4 parts of rotten mosses ash.
3. the preparation method of organic matter decomposing inoculant as claimed in claim 1 or 2, specific as follows:
The first, the preparation of bacillus subtilis fermentation liquor:
(1) preparation of seed liquor: the thallus that one connects collarium bacillus subtilis is scraped from slant medium and is inoculated into liquid training
It supports in base, shaking table culture 12h, revolving speed 200r/min, temperature is 30 DEG C, obtains the seed liquor of bacillus subtilis；
Bacillus subtilis fluid nutrient medium (g/L): tryptone 10, yeast extract 5, NaCl 10, water complements to 1L；
(2) preparation of fermentation liquid: the seed liquor that step (1) obtains is inoculated into fermentor with the inoculum concentration of 10% (v/v),
Revolving speed is that 100-120 turns/min, and temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments, ferments under conditions of/h
Time is 18-20h to get bacillus subtilis fermentation liquor；
Fermentation medium (g/L): corn flour 12.5, glucose 5, beancake powder 20.1, CaCO36.9, ammonium sulfate 1, K2HPO4
0.3, epsom salt 0.2, manganese sulfate monohydrate 0.2, water complements to 1L；
The second, the preparation of unwrapping wire bacteria solid fermentation object:
(1) preparation of seed liquor: being inoculated into fluid nutrient medium from the thallus that scraping one connects collarium actinomyces on slant medium,
Shaking table culture 12h, revolving speed 220r/min, temperature are 32 DEG C, obtain the seed liquor of actinomyces；
Actinomyces fluid nutrient medium (g/L): potassium nitrate 1, dipotassium hydrogen phosphate 0.5, epsom salt 0.5, sodium chloride 0.5, seven water
Ferrous sulfate 0.01, water complements to 1L；
(2) preparation of fermentation liquid:
The seed liquor that step (1) obtains is inoculated into fermentor with the inoculum concentration of 10% (v/v), is 120 turns/min in revolving speed,
Temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is 30-32h to get unwrapping wire
Fermented liquid；
Actinomyces liquid fermentation medium (g/L): glucose 1, yeast extract 0.4, peptone 0.4, potassium dihydrogen phosphate 0.2, phosphoric acid
Hydrogen dipotassium 0.4, epsom salt 0.05, water complements to 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into unwrapping wire with 10% inoculum concentration (m/m)
In bacteria solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, the item of height 30-40cm is made in the material of mixing
Pile；3. fermentation management: when temperature of charge no longer improves after heating up, turning continues culture 3-5 days to get actinomyces solid hair
Ferment object；
Unwrapping wire bacteria solid fermentation culture medium (being calculated in mass percent): chicken manure (dry weight) 50%, mushroom residue 50% mix；
The preparation of third, streptococcus acidi lactici fermented solution:
(1) preparation of seed liquor: being inoculated into fluid nutrient medium from the thallus that scraping one connects collarium lactic acid bacteria on slant medium,
Shaking table culture 12h, revolving speed 220r/min, temperature are 30 DEG C, obtain the seed liquor of lactic acid bacteria；
Lactic acid bacteria fluid nutrient medium (g/L): peptone 10.0, powdered beef 5.0, yeast powder 4.0, glucose 20.0, Tween 80
1.0mL, dipotassium hydrogen phosphate 2.0, sodium acetate 5.0, Triammonium citrate 2.0, magnesium sulfate 0.2, manganese sulfate 0.05, water complements to 1L；
(2) preparation of fermentation liquid: the seed liquor that step (1) obtains is inoculated into fermentor with the inoculum concentration of 10% (v/v),
Revolving speed is that 100-120 turns/min, and temperature is 28 DEG C, 200L fermentor, ventilatory capacity 6m3/ h, pH are sent out under conditions of being 6-6.5
Ferment, fermentation time are 22-24h to get streptococcus acidi lactici fermented solution；
Lactobacillus-fermented culture medium (g/L): peptonized milk 1, yeast extract 0.5, potassium dihydrogen phosphate 0.2, glucose 1, tomato are leached
Powder 0.25, Tween 80 0.1, water complements to 1L；
4th, the preparation of Aspergillus oryzae solid culture:
(1) preparation of seed liquor: the preparation of seed liquor: the thallus that one connects collarium aspergillus oryzae is scraped from slant medium and is inoculated into
In fluid nutrient medium, shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of aspergillus oryzae；
Aspergillus oryzae fluid nutrient medium (g/L): urea 0.4, peptone 0.35, magnesium sulfate 0.09, sodium chloride 0.04, ferric sulfate
0.05, potassium dihydrogen phosphate 0.18, water complement to 1L；
(2) preparation of fermentation liquid: the seed liquor that step (1) obtains is inoculated into fermentor with the inoculum concentration of 10% (v/v),
Revolving speed is 120 turns/min, and temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is
28-30h is to get aspergillus oryzae fermentation liquid；
Aspergillus oryzae fermentation medium (g/L): urea 4, peptone 3, dipotassium hydrogen phosphate 1.6, magnesium sulfate 0.7, sodium chloride 0.5, sulphur
Sour iron 0.5. water supplies 1L；
(3) solid culture: 1. ingredient is fermented: the fermentation liquid that step (2) obtain is inoculated into the inoculum concentration (m/m) of 10-15%
In aspergillus oryzae solid fermentation culture medium, mix；2. heap processed: length 10m, wide 2-4m, height 30-40cm is made in the material of mixing
Pile；3. fermentation management: for temperature of charge not when improving, it is solid to get aspergillus oryzae that turning continues culture 3-5 days after heating up
Body fermentation material；
Aspergillus oryzae solid fermentation culture medium is mushroom residue；
5th, the preparation of saccharomycetes to make fermentation liquid
(1) preparation of seed liquor: being inoculated into fluid nutrient medium from the thallus that scraping one connects collarium saccharomycete on slant medium,
Shaking table culture 12h, revolving speed 220r/min, temperature are 28 DEG C, obtain the seed liquor of saccharomycete；
Saccharomycete fluid nutrient medium (g/L): yeast extract 1, glucose 2, peptone 2, water complements to 1L；
(2) preparation of fermentation liquid: the seed liquor that step (1) obtains is inoculated into fermentor with the inoculum concentration of 10% (v/v),
Revolving speed is 120 turns/min, and temperature is 30 DEG C, 200L fermentor, ventilatory capacity 6m3It ferments under conditions of/h, fermentation time is
12-14h is to get saccharomycetes to make fermentation liquid；
Saccharomycetes to make fermentation culture medium (g/L): peptone 20, glucose 20, potassium dihydrogen phosphate 1, epsom salt 0.5, sodium chloride
5, add water to 1L, pH 7.0-7.2；
6th, by bacillus subtilis fermentation liquor, unwrapping wire bacteria solid fermentation object, streptococcus acidi lactici fermented solution, Aspergillus oryzae solid culture,
Saccharomycetes to make fermentation liquid and rotten mosses ash mix in proportion to obtain the final product.
4. according to the method described in claim 3, it is characterized in that, in the preparation of bacillus subtilis fermentation liquor, revolving speed 110
Turn/min, fermentation time 19h.
5. according to the method described in claim 3, it is characterized in that, in the preparation of actinomycetes fermentation liquor, fermentation time 31h.
6. according to the method described in claim 3, it is characterized in that, in the preparation of streptococcus acidi lactici fermented solution, revolving speed is 110 turns/
Min, pH 6.2, fermentation time 23h.
7. according to the method described in claim 3, it is characterized in that, in the preparation of aspergillus oryzae fermentation liquid, fermentation time 29h.
8. according to the method described in claim 3, it is characterized in that, in the preparation of saccharomycetes to make fermentation liquid, fermentation time 13h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710648274.5A CN107365725B (en) | 2017-08-01 | 2017-08-01 | A kind of organic matter decomposing inoculant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710648274.5A CN107365725B (en) | 2017-08-01 | 2017-08-01 | A kind of organic matter decomposing inoculant and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107365725A CN107365725A (en) | 2017-11-21 |
| CN107365725B true CN107365725B (en) | 2019-09-10 |
Family
ID=60308725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710648274.5A Active CN107365725B (en) | 2017-08-01 | 2017-08-01 | A kind of organic matter decomposing inoculant and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107365725B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022096723A1 (en) * | 2020-11-09 | 2022-05-12 | Sacco Srl | Composition for bio stimulation of plants |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828699A (en) * | 2017-12-13 | 2018-03-23 | 潍坊市华滨生物科技有限公司 | A kind of farm composite bacteria preparation and preparation method |
| CN107881136A (en) * | 2017-12-27 | 2018-04-06 | 李晓明 | The method of composite bacterial and preparation method thereof and degraded animal waste |
| CN108795812A (en) * | 2018-06-25 | 2018-11-13 | 湖南泰谷生态工程有限公司 | A kind of compost decomposing agent and the preparation method and application thereof |
| CN108893433B (en) * | 2018-07-20 | 2021-06-29 | 湖北武当动物药业有限责任公司 | Crop straw waste leavening agent and preparation method and application thereof |
| CN108997034A (en) * | 2018-08-10 | 2018-12-14 | 成都惠森生物技术有限公司 | Decomposed starting agent of one kind and preparation method thereof |
| CN112573946A (en) * | 2020-12-01 | 2021-03-30 | 沈阳农业大学 | Organic waste decomposition agent and bio-organic fertilizer for improving saline-alkali soil |
| CN113548916A (en) * | 2021-06-25 | 2021-10-26 | 山东农大肥业科技有限公司 | Special decomposition agent containing oxygenation agent and humic acid for cage-rearing chicken manure and application thereof |
| CN113735652A (en) * | 2021-08-27 | 2021-12-03 | 河北昱鼎生物科技有限公司 | Efficient organic material decomposing agent and preparation method and application thereof |
| CN117285382B (en) * | 2023-11-22 | 2024-04-05 | 海南热带海洋学院 | Method for multi-strain fermentation of fish manure and waste low-odor fertilizer based on neural network |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219570A (en) * | 2011-05-09 | 2011-10-19 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN103333846A (en) * | 2013-07-22 | 2013-10-02 | 四川沃达丰生物科技有限公司 | Organic material decomposition agent |
| CN104692961A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Microorganism organic material decomposition agent as well as preparation method and applications thereof |
| CN105505826A (en) * | 2016-01-05 | 2016-04-20 | 浙江大学 | Livestock excrement and sewage compound microbial deodorizer and preparation method thereof |
-
2017
- 2017-08-01 CN CN201710648274.5A patent/CN107365725B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219570A (en) * | 2011-05-09 | 2011-10-19 | 成都市泰兴生态农业有限公司 | Bio-organic fertilizer produced by using livestock manure |
| CN103333846A (en) * | 2013-07-22 | 2013-10-02 | 四川沃达丰生物科技有限公司 | Organic material decomposition agent |
| CN104692961A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Microorganism organic material decomposition agent as well as preparation method and applications thereof |
| CN105505826A (en) * | 2016-01-05 | 2016-04-20 | 浙江大学 | Livestock excrement and sewage compound microbial deodorizer and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022096723A1 (en) * | 2020-11-09 | 2022-05-12 | Sacco Srl | Composition for bio stimulation of plants |
| NL2026852B1 (en) * | 2020-11-09 | 2022-07-04 | Sacco Srl | Composition for bio stimulation of plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107365725A (en) | 2017-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107365725B (en) | A kind of organic matter decomposing inoculant and preparation method thereof | |
| CN103483020B (en) | A kind of biological organic fertilizer and preparation method preventing and treating soil insect | |
| CN102910988B (en) | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer | |
| CN105420156A (en) | Bacillus amyloliquefaciens with phosphate solubilizing, disease preventing and growth promoting functions and application thereof | |
| CN105296394A (en) | Microbial decomposition agent for animal wastes and straws and preparation method thereof | |
| CN106518284A (en) | Bio-organic fertilizer for dragon fruit planting and preparing method thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN1872814A (en) | Method for preparing insect repelling organic fertilizer | |
| CN107840762A (en) | A kind of method that biological organic fertilizer is made using cow dung | |
| CN107867931A (en) | Using White mushroom planting material of the padding for pig sty as primary raw material of giving up | |
| CN104276874A (en) | Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase | |
| CN109852565A (en) | A kind of salt-soda soil composite modifying agent and its method of administration | |
| CN107266184A (en) | Lilium brownii var viridulum cultivation matrix and preparation method thereof | |
| CN107325990A (en) | A kind of complex microorganism decomposing agent and its purposes for tobacco cultivation | |
| CN101372425A (en) | Microbial fertilizer and preparation thereof | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN105462878A (en) | Livestock excrement efficient decomposition agent and fermentation method thereof | |
| CN103980010A (en) | Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant | |
| CN108947600A (en) | The preparation method and organic fertilizer of organic fertilizer | |
| CN107879852A (en) | A kind of edaphon modifying agent and preparation method thereof | |
| CN107646616A (en) | A kind of preparation method of floriculture substrate | |
| CN106316687A (en) | Organic fertilizer giving consideration to crop nutrient needs and soil ecological improvement and preparation method thereof | |
| CN110117565A (en) | A kind of biology decomposing agent and its application in compost | |
| CN105859381A (en) | Compound microbial-fermented organic fertilizer and preparation method therefor | |
| CN106045677A (en) | Method for preparing organic compound biological fertilizer special for fruit trees |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ding Fangjun Inventor after: Yu Jian Inventor after: Chen Shigeng Inventor after: Song Yiling Inventor after: Xiao Chengze Inventor before: Ding Fangjun Inventor before: Xiao Chengze Inventor before: Yu Jian Inventor before: Chen Shigeng Inventor before: Song Yiling |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 271699 Feicheng high tech Development Zone, Tai'an, Shandong Patentee after: Shandong Nongda Feiye Technology Co.,Ltd. Address before: 271600 Feicheng High-tech Development Zone, Taian City, Shandong Province Patentee before: SHANDONG AGRUNIV FERT SCITECH Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: No. 249, Chuangye Road, High tech Zone, Feicheng City, Tai'an City, Shandong Province, 271600 Patentee after: Shandong Nongda Feiye Technology Co.,Ltd. Country or region after: China Address before: 271699 Feicheng high tech Development Zone, Tai'an, Shandong Patentee before: Shandong Nongda Feiye Technology Co.,Ltd. Country or region before: China |