CN107879852A - A kind of edaphon modifying agent and preparation method thereof - Google Patents
A kind of edaphon modifying agent and preparation method thereof Download PDFInfo
- Publication number
- CN107879852A CN107879852A CN201711228377.2A CN201711228377A CN107879852A CN 107879852 A CN107879852 A CN 107879852A CN 201711228377 A CN201711228377 A CN 201711228377A CN 107879852 A CN107879852 A CN 107879852A
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- Prior art keywords
- bacillus
- edaphon
- modifying agent
- carrier
- soil
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
Abstract
The present invention relates to a kind of edaphon modifying agent and preparation method thereof, belong to technical field of soil remediation.Edaphon modifying agent includes functional microorganism and carrier, and the mass ratio of the two is：Functional microorganism 20% 40%, carrier 60% 80%.The functional microorganism is bacillus laterosporus Bacillus laterosporus,Bacillus megaterium Bacillus megaterium,Bacillusmusilaginosiengineering Bacillus mucilaginosus,Bacillus licheniformis Bacillus licheniformis,Streptomyces jingyangensis Streptomyces chingyangensis,Lactobacillus plantarum Lactobacillus plantarum,Bacillus circulans Bacillus circulans,Pseudomonas stutzeri Pseudomonas stutzeri,One or more in Trichoderma viride Trichoderma viride.Edaphon modifying agent of the present invention is enriched with using the functional microorganism culture filtered out in specific soil, and invest carrier and be prepared, there is very strong adaptability and colonization ability to soil, Soil Microorganism colony balance can quickly be adjusted, rehabilitating soil structure, fundamentally solve the Lands such as soil hardening, residues of pesticides.
Description
Technical field
The present invention relates to a kind of edaphon modifying agent and preparation method thereof, belong to technical field of soil remediation.
Background technology
The many regional arable lands in China are seriously damaged due to long-term predation formula farming, nutrient loss, farmland quality.Change
The invention and use of fertilizer promote the development of agricultural within a certain period of time, but long-term use also highlights many drawbacks.Excessively according to
Rely the use of chemical fertilizer, and chemical fertilizer utilization ratio is relatively low, make arable land generally occur degenerating, arable layer shoals, the hardening soil, environment are dirty
A series of problems, such as dye.For the sustainable development of agricultural, reach high yield, high-quality, efficient purpose, the improvement and reparation of soil
It is to draw attention.
Current physical modifying agent can only change the physical behavior of soil, but not tackle the problem at its root, and
Improve the Micro Ecosystem of crop rhizosphere soils using beneficial microbe, the material that crops are difficult by changes into can quilt
The effective nutriment utilized, rehabilitating soil structure, it is really achieved the purpose of rehabilitating soil.However, the microorganism of in the market changes
Good dose there is also it is certain the problem of, such as microbe species are too many, not only cost increase, and cause nutrient competition, suppression mutually
System；Microorganism is not the natural microbial in soil, and effect is obvious but can not be colonized in soil, it is necessary to be continuously replenished；It is micro-
After biology is added in soil, because growing environment is severe and shortage nutrition is poor, growth and breeding is slow, can not play a role；
Part nutriment is with the addition of in some microorganism modifying agents, but also increases cost simultaneously, and nutrition is unbalanced etc..
The content of the invention
In view of the foregoing defects the prior art has, the present invention proposes a kind of edaphon modifying agent and its preparation side
Method.
The present invention is realized using following technical scheme：
A kind of edaphon modifying agent, including functional microorganism and carrier, the functional microorganism are side spore gemma bar
Bacterium Bacillus laterosporus, bacillus megaterium Bacillus megaterium, bacillusmusilaginosiengineering
Bacillus mucilaginosus, bacillus licheniformis Bacillus licheniformis, Streptomyces jingyangensis
Streptomyces chingyangensis, Lactobacillus plantarum Lactobacillus plantarum, Bacillus circulans
Bacillus circulans, Pseudomonas stutzeri Pseudomonas stutzeri, Trichoderma viride Trichoderma
One or more in viride, the functional microorganism is screened from soil.Strain used is all selected surging strain, sieve
Selected from specific environment soil, competitive relation is not present between each strain, it is strong to soil suitability, can quick growth and breeding,
And colonized in soil.
The mass ratio of the functional microorganism and carrier is：Functional microorganism 20%-40%, carrier 60%-80%.
The present invention is using microorganism and the method for plant combined reparation, the metabolism secreted in strain growth course used
Product can promote the growth of crop root system, so as to which the phosphorus, the element such as potassium that promote to deposit in soil absorb, accelerate soil
Earth repairs speed.The crop root remained in simultaneously in soil can also increase the content of organic matter in soil.
The functional microorganism and mass ratio are bacillusmusilaginosiengineering 10%-30%, Streptomyces jingyangensis 10%-
30%th, Lactobacillus plantarum 30%-50%, Trichoderma viride 10%-30%, the inoculating proportion is especially suitable for the slight salinization of soil of soil
Situation, there is preferable repairing effect.
Technical scheme is not limited to a kind of land type, can have difference for different types of land type
Combination：In the case of there is soil hardening, preferably strain is bacillus megaterium, bacillusmusilaginosiengineering, Jingyang
Streptomycete, Trichoderma viride；For soil pest and disease damage serious situation, preferably strain be bacillus licheniformis, bacillus laterosporus,
Bacillusmusilaginosiengineering, Trichoderma viride；In the case of residues of pesticides are exceeded, preferably strain is Pseudomonas stutzeri, huge bud
Spore bacillus, Lactobacillus plantarum, bacillus licheniformis.
The raw material of the carrier includes animal wastes and plant material, and the animal wastes are included in chicken manure, duck excrement, pig manure
One or more, the plant material includes wood chip, stalk, the one or more in rice husk.
The raw material of the carrier is chicken manure and stalk, and after fermented decomposed deodorization, low temperature drying, which crushes, to be used.With chicken manure etc.
For the plant material such as animal wastes and stalk as carrier, plant material can increase the content of organic matter in soil, increase fertility,
And containing substantial amounts of unemployed nutriment in the animal wastes such as chicken manure, it is abundant so as to be provided for the growth of microorganism
Nutritional ingredient, ensure that microorganism persistently plays a role.
The functional microorganism is powdery, and the viable bacteria amount of every gram of pulvis is 2 × 109-1×1010。
The preparation process of the carrier includes：
1) crush and mix：Plant material is crushed and mixed with animal wastes；
2) it is inoculated with：Inoculation fermentation microbial inoculum；
3) ferment.
After can first plant material be crushed, then mix with animal wastes, implement powder again after can also the two be mixed
It is broken.
The mass ratio of the support material is：
Animal wastes 10%-60%
Plant material 10%-60%
Fermenting agent 5%-10%
Water 0%-40%.
The fermenting agent includes the one or more in enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, aspergillus niger.
The preparation process of the carrier also includes the baking step after fermentation and the pulverising step after drying.The baking
Dry step meets condition：50 DEG C -70 DEG C of drying temperature, moisture 13%-17% after drying.The fermentation step meets bar
Part：The moisture of wet stock is controlled in 30%-50%, 30 DEG C -40 DEG C of fermentation temperature, fermentation time 3-5 days before fermentation.This hair
Bright used carrier is rich in nutritional ingredient, by the innoxious decomposed processing of beneficial bacterium, with a large amount of beneficial viable bacterias and its metabolism production
Thing, abundant nutriment can be provided for functional microorganism, ensure needed for microbial growth, to enable microorganism lasting
Play repair.
Embodiment
In order that the object of the invention, technical scheme are more clearly understood, the present invention is made below by embodiment further
Describe in detail.
Embodiment one
Functional microorganism selection bacillus laterosporus, bacillus megaterium, bacillusmusilaginosiengineering, bacillus licheniformis,
Streptomyces jingyangensis, Lactobacillus plantarum, Bacillus circulans and Trichoderma viride, are mixed according to equal proportion, and every gram of pulvis is lived after mixing
Bacterium amount is 8.37 × 109, the functional microorganism is screened from soil.
Carrier presses 1 by chicken manure and stalk:1 ratio mixes, and is inoculated with 3% bacillus subtilis and 3% aspergillus niger, moisture
Content control is fermented at 30 DEG C 40%.Temperature is first raised and reduced afterwards in fermentation process, decomposed speed, after fermentation in 3 days
Terminate.Because strain can produce resistant to elevated temperatures gemma or spore, dry under the conditions of 70 DEG C, then crush.On carrier sense organ
Stink disappears substantially, measures every gram of siccative viable bacteria amount as 1.46 × 109。
By functional microorganism and carrier according to mass ratio 3：7 ratio mixing, is made microbe soil conditioner.
Embodiment two
The key influence factor that functional microorganism used plays a role in microbe soil conditioner is to fit soil
Should be able to power, realize the stable growth and breeding of flora.The present embodiment is by the strain of microbe soil conditioner with coming from other ways
The identical strain in footpath carries out contrast test, the adaptability and stability of authentication function flora.
Three kinds of bacillus licheniformis, Lactobacillus plantarum and Trichoderma viride microorganisms are chosen as Experimental Comparison bacterial strain, experiment
Three kinds of microorganisms that group is selected screened from soil, three kinds of microorganisms that control group is selected both from other approach, other
Part is identical.Carrier presses 1 from chicken manure and stalk:1 ratio mixes, and is inoculated with 3% bacillus subtilis and 3% aspergillus niger, water
The control of point content is fermented at 30 DEG C 40%.Fermentation ends high temperature is dried, and fermented bacterium contained in carrier is all killed
Go out, then by two groups of microbial bacterial agents respectively with carrier according to 30%：70% ratio mixing, is made microbe soil improvement
Agent.
Soil is subjected to sterilization pretreatment first, control group and test group microbe soil conditioner are then applied to soil
In earth, microorganism live bacteria amount is measured by sampling after inoculation, places 15 days under natural environment and is measured by sampling again, comparing result is such as
Under：
The soil suitability of table 1 tests viable bacteria amount measurement result unit：Hundred million/gram
Found out by result above, although microbial species belong to identical, the strain differences screened from varying environment are larger, right
According to group, there have been mortality, the survival rate of wherein Lactobacillus plantarum in 15 days there was only 54%；And test group survival rate is all
More than 90%, and bacillus licheniformis have also appeared and rise appreciably.Therefore, the microbial strains screened from soil are production
The optimum strain of soil conditioner, bacterial activity can be farthest kept, acted on more persistently effective.
Embodiment three
Functional microorganism selection bacillus megaterium, bacillusmusilaginosiengineering, Streptomyces jingyangensis and Trichoderma viride, according to matter
Amount is than being 2：2：3：3 ratio mixing, every gram of pulvis viable bacteria amount is 7.24 × 10 after mixing9。
Carrier presses 2 by chicken manure and stalk:1 ratio mixes, and is inoculated with 5% enterococcus faecalis and 3% saccharomyces cerevisiae, moisture contains
Amount control is fermented at 35 DEG C 30%.Temperature is first raised and reduced afterwards in fermentation process, and decomposed speed is relatively slow, is sent out after 5 days
Ferment terminates.Due to strain non-refractory used, dry under the conditions of 50 DEG C, then crush.Stink is wholly absent on carrier sense organ,
And wine flavour is carried, every gram of siccative viable bacteria amount is measured as 9.83 × 108。
According to mass ratio it is 2 by functional microorganism and carrier：3 ratio mixing, is made microbe soil conditioner.
Microbe soil conditioner is applied to the corn field for soil hardening problem occur, before ploughing soil, according to
The amount of application of 10kg/ mus is uniformly sprinkling upon ground, is then turned over, and chooses the adjacent plot of condition identical as control, right
The carrier by sterilization treatment of equivalent is only used according to group.Experiment process is conventionally managed, normal irrigation.Experiment
The content of organic matter, available phosphorus contents before starting in sampling and measuring soil, quick-acting potassium content, and the microorganism that modifying agent is brought into
Total amount.After experiment one month, it is measured by sampling.Compared with control group plot, soil organic matter content improves 24.3%, rapid available phosphorus
11.6% and 17.1% has been respectively increased with available potassium, Microbial survival rate 91.8%, has illustrated that microorganism modifying agent was using
Stability is fine in journey, and soil remediation positive effect.From the point of view of plant, test group growth is neat, and well developed root system.
After harvest, determination test group yield improves 18.4% to control group.
Example IV
Functional microorganism selection bacillusmusilaginosiengineering, Streptomyces jingyangensis, Lactobacillus plantarum and Trichoderma viride, according to quality
Than for 1:1:2:1 ratio mixing, every gram of pulvis viable bacteria amount is 8.44 × 10 after mixing9。
Carrier presses 1 by chicken manure and stalk:2 ratios mix, and are inoculated with 5% enterococcus faecalis and 3% aspergillus niger, moisture
Control is fermented at 35 DEG C 50%.Temperature is first raised and reduced afterwards in fermentation process, and decomposed speed is relatively slow, after fermentation in 4 days
Terminate.Material is dried under the conditions of 50 DEG C after fermentation, is then crushed.Stink is wholly absent on carrier sense organ, and carries tart flavour, is surveyed
It is 1.36 × 10 to obtain every gram of siccative viable bacteria amount9。
According to mass ratio it is 2 by functional microorganism and carrier:3 ratio mixing, is made microbe soil conditioner.
Microbe soil conditioner is subjected to application test in the Vegetable Base that the salinization of soil be present, with greenhouse kind
Eggplant, because soil is slightly saline and alkaline, have impact on tomato mouthfeel as subjects.Experiment process is during applying fertilizer to the subsoil, and is incited somebody to action
Microbe soil conditioner uses according to the amount of application of 20kg/ mus, and test period is one month, when on-test and knot
It is measured by sampling during beam.Show have using microbe soil conditioner to slight salt affected soil and significantly repair by testing result
Effect, soil salt content drop to 0.19% by 0.44%, and soil pH value drops to 6.8 by 7.6, and harvest tomato mouthfeel substantially changes
It is kind.
Claims (10)
1. a kind of edaphon modifying agent, it is characterised in that including functional microorganism and carrier, the functional microorganism is side
Spore bacillus laterosporus, bacillus megaterium Bacillus megaterium, gel-shaped gemma bar
Bacterium Bacillus mucilaginosus, bacillus licheniformis Bacillus licheniformis, Streptomyces jingyangensis
Streptomyces chingyangensis, Lactobacillus plantarum Lactobacillus plantarum, Bacillus circulans
Bacillus circulans, Pseudomonas stutzeri Pseudomonas stutzeri or Trichoderma viride Trichoderma
One or more in viride, the functional microorganism is screened from soil.
2. a kind of edaphon modifying agent according to claim 1, it is characterised in that the functional microorganism and carrier
Mass ratio be：
Functional microorganism 20%-40%
Carrier 60%-80%.
A kind of 3. edaphon modifying agent according to claim 2, it is characterised in that the quality of the functional microorganism
Percentage is bacillusmusilaginosiengineering 10%-30%, Streptomyces jingyangensis 10%-30%, Lactobacillus plantarum 30%-50%, green
Trichoderma 10%-30%.
4. a kind of edaphon modifying agent according to claim 1, it is characterised in that the raw material of the carrier includes dynamic
Thing excrement and plant material, the animal wastes include the one or more in chicken manure, duck excrement or pig manure, the plant material bag
Include the one or more in wood chip, stalk or rice husk.
5. a kind of edaphon modifying agent according to claim 4, it is characterised in that the raw material of the carrier is chicken manure
And stalk, after fermented decomposed deodorization, low temperature drying, which crushes, to be used.
6. a kind of edaphon modifying agent according to claim 1, it is characterised in that the functional microorganism is powder
Shape, the viable bacteria amount of every gram of pulvis is 2 × 109-1×1010。
A kind of 7. preparation method of edaphon modifying agent as claimed in claim 4, it is characterised in that the system of the carrier
Standby step includes：
1) crush and mix：Plant material is crushed and mixed with animal wastes；
2) it is inoculated with：Inoculation fermentation microbial inoculum；
3) ferment.
8. the preparation method of edaphon modifying agent according to claim 7, it is characterised in that the raw material of the carrier
Mass ratio is：
Animal wastes 10%-60%
Plant material 10%-60%
Fermenting agent 5%-10%
Water 0%-40%.
9. the preparation method of edaphon modifying agent according to claim 8, it is characterised in that the fermenting agent bag
Include the one or more in enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae or aspergillus niger.
10. the preparation method of edaphon modifying agent according to claim 9, it is characterised in that the system of the carrier
Standby step also includes the baking step after fermentation and the pulverising step after drying；The baking step meets condition：Drying
- 70 DEG C of temperature 50 C, moisture 13%-17% after drying；The fermentation step meets condition：The moisture of wet stock before fermentation
Content is controlled in 30%-50%, 30 DEG C -40 DEG C of fermentation temperature, fermentation time 3-5 days.
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Cited By (9)
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| CN109020699A (en) * | 2018-09-06 | 2018-12-18 | 中海新世纪量子生物科技（深圳）有限责任公司 | Soil remediation leavening, soil remediation microbial inoculum, preparation method and soil remediation method |
| CN109517769A (en) * | 2019-01-25 | 2019-03-26 | 吕树平 | A kind of soil remediation complex microbial inoculum and its preparation method and application |
| CN111362742A (en) * | 2018-12-26 | 2020-07-03 | 王虎 | Preparation method of special nutrient fertilizer for vegetable planting |
| CN111718876A (en) * | 2020-06-29 | 2020-09-29 | 北京中外建建筑设计有限公司 | Compound microbial agent and preparation method and application thereof |
| CN112458010A (en) * | 2020-11-21 | 2021-03-09 | 东北农业大学 | Solid compound microbial agent for improving saline-alkali soil and application thereof |
| CN112573971A (en) * | 2020-12-07 | 2021-03-30 | 沈阳农业大学 | Soil microorganism improver containing furnace ash and application thereof |
| CN112625690A (en) * | 2020-12-01 | 2021-04-09 | 沈阳农业大学 | Composite microbial porous material for inhibiting saline-alkali soil salt return and application thereof |
| CN113897199A (en) * | 2021-10-29 | 2022-01-07 | 深圳市宏大建设集团有限公司 | Repairing agent for eliminating soil harmful bacteria and preparation method thereof |
| CN114804975A (en) * | 2022-03-09 | 2022-07-29 | 美丽华夏生态环境科技有限公司 | Microbial soil conditioner for northwest arid region and preparation method thereof |
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| CN112625690A (en) * | 2020-12-01 | 2021-04-09 | 沈阳农业大学 | Composite microbial porous material for inhibiting saline-alkali soil salt return and application thereof |
| CN112573971A (en) * | 2020-12-07 | 2021-03-30 | 沈阳农业大学 | Soil microorganism improver containing furnace ash and application thereof |
| CN113897199A (en) * | 2021-10-29 | 2022-01-07 | 深圳市宏大建设集团有限公司 | Repairing agent for eliminating soil harmful bacteria and preparation method thereof |
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Application publication date: 20180406 |