CN105886438A

CN105886438A - Composition or composite bacterium agent for treating sludge

Info

Publication number: CN105886438A
Application number: CN201610308774.XA
Authority: CN
Inventors: 陈五岭; 黄敏刚
Original assignee: 陈五岭
Current assignee: SHANXI WENLING MICRO-BIOLOGY TECHNOLOGY Co.,Ltd.
Priority date: 2016-05-11
Filing date: 2016-05-11
Publication date: 2016-08-24
Anticipated expiration: 2036-05-11
Also published as: CN105886438B

Abstract

The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises a composition of at least six or seven strains from bacillus subtilis, bacillus megatherium, pseudomonad, sphingobacterium thalpophilum, candida tropicalis, bacillus firmus and lactobacillus plantarum. The application includes sludge treatment.

Description

For processing compositions or the composite bacteria agent capable of mud

Technical field

The application belongs to field of environmental improvement.Specifically, the application relates to process compositions or the compound bacteria of mud Agent.

Background technology

Recently as the attention of our Environmental protection work, a lot of local governments are to local river and row Dirty closed conduit has carried out dredging governance, the most also creates substantial amounts of mud.Rivers and lakes mud complicated components, rich in organic matter And moisture content is high, with pungent odor, have possibly together with substantial amounts of heavy metal ion, therefore arbitrarily stacking does not only take up a large amount of soil Ground, is also easy to cause secondary pollution simultaneously.If rivers and lakes mud can not process in time, the foul odour come out is to attached The life of nearly resident will also result in the biggest impact.

At present the repair mode of rivers and lakes mud mainly has a following two: physics mode and biological mode.Peripheral doses Mode mainly by the method for machinery mummification, geotechnique's cloth bag mummification or mud nature mummification, rivers and lakes mud is carried out Processed, then carries out subsequent treatment.Bioremediation technology refers to that the vital metabolic activity by microorganism, plant etc. is dropped Solve, absorb, convert harmful substance in the mud of rivers and lakes, thus reach the purpose that in the mud of rivers and lakes, harmful substance is removed. Microbial treatment recovery technique is simple to operate, can carry out substantial amounts of rivers and lakes mud and administer.

Summary of the invention

On the one hand, the application provides a kind of compositions, comprises bacillus subtilis (Bacillus subtilis), huge Bacillus cereus (Bacillus megaterium), pseudomonas (Pseudomonadaceae), mesophilic Sphingobacterium (Sphingobacterium thalpophilum), candida tropicalis (Candida tropicalis), bacillus firmus In (Bacillus firmus) and Lactobacillus plantarum (Lactobacillus plantarum) at least six kinds.

In one embodiment, described compositions comprises bacillus subtilis, bacillus megaterium, pseudomonas, heat Band candida mycoderma, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, mesophilic Sphingobacterium, Candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, mesophilic Sphingobacterium, heat Band candida mycoderma, bacillus firmus and Lactobacillus plantarum, or, bacillus megaterium, pseudomonas, mesophilic Sphingobacterium, Candida tropicalis, bacillus firmus and Lactobacillus plantarum.

In one embodiment, described compositions comprise bacillus subtilis, bacillus megaterium, pseudomonas, addicted to Temperature Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum.

In one embodiment, described compositions comprises compositions A and compositions B, and described compositions A comprises hay bud Spore bacillus, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium, described compositions B comprises candida tropicalis, strong Bacillus cereus and Lactobacillus plantarum.

In one embodiment, described compositions comprises compositions A and compositions B, and described compositions A comprises hay bud Spore bacillus, bacillus megaterium and pseudomonas, bacillus subtilis, bacillus megaterium and mesophilic Sphingobacterium, hay Bacillus cereus, pseudomonas and mesophilic Sphingobacterium, or, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium；Institute State compositions B and comprise candida tropicalis, bacillus firmus and Lactobacillus plantarum.

On the other hand, the application provides a kind of composite bacteria agent capable, and its composition comprises bacillus subtilis (Bacillus Subtilis), bacillus megaterium (Bacillus megaterium), pseudomonas (Pseudomonadaceae), mesophilic sheath Ammonia alcohol bacillus (Sphingobacterium thalpophilum), candida tropicalis (Candida tropicalis), strong In bacillus cereus (Bacillus firmus) and Lactobacillus plantarum (Lactobacillus plantarum) at least six kinds.

In one embodiment, described composite bacteria agent capable comprise bacillus subtilis, bacillus megaterium, pseudomonas, Candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, mesophilic sphingol bar Bacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, mesophilic sphingol bar Bacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, or, bacillus megaterium, pseudomonas, mesophilic sphingol Bacillus, candida tropicalis, bacillus firmus and Lactobacillus plantarum；Or comprise the cultivation of six kinds of bacterial strains of combinations thereof Thing.

In one embodiment, described composite bacteria agent capable comprise bacillus subtilis, bacillus megaterium, pseudomonas, Mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, or comprise the training of these seven kinds of bacterial strains described Support thing.

In one embodiment, described composite bacteria agent capable is made up of above-mentioned seven kinds of bacterial strains.

In one embodiment, described composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B, described composite bacteria agent capable A bag Containing bacillus subtilis, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium, or the cultivation of described these four bacterial strain Thing；Described composite bacteria agent capable B comprises candida tropicalis, bacillus firmus and Lactobacillus plantarum, or described these three bacterial strain Culture.

In one embodiment, described composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B, described composite bacteria agent capable A bag Containing bacillus subtilis, bacillus megaterium and pseudomonas, bacillus subtilis, bacillus megaterium and mesophilic sphingol bar Bacterium, bacillus subtilis, pseudomonas and mesophilic Sphingobacterium, or, bacillus megaterium, pseudomonas and mesophilic sphingol Bacillus, or the culture of three kinds of bacterial strains of described combinations thereof；Described composite bacteria agent capable B comprises candida tropicalis, strong bud Spore bacillus and Lactobacillus plantarum, or the culture of described these three bacterial strain.

In one embodiment, described composite bacteria agent capable comprise composite bacteria agent capable A and composite bacteria agent capable B, described composite bacteria agent capable A by Bacillus subtilis, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium these four bacterial strain composition；Described composite bacteria agent capable B is made up of candida tropicalis, bacillus firmus and Lactobacillus plantarum these three bacterial strain.

On the other hand, the application further relates to the preparation method of a kind of composite bacteria agent capable, including:

By bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), Pseudomonas (Pseudomonadaceae), mesophilic Sphingobacterium (Sphingobacterium thalpophilum), the torrid zone Candida mycoderma (Candida tropicalis), bacillus firmus (Bacillus firmus) and Lactobacillus plantarum (Lactobacillus plantarum) cultivates at least six kinds respectively；And obtain the respective cultivation of described bacterial strain Thing, and described culture is mixed according to combination, obtain described composite bacteria agent capable；

Optionally, above-mentioned cultivation is solid culture, Semi-solid cell culture or liquid culture.

In one embodiment, by described bacillus subtilis, bacillus megaterium, pseudomonas, the false silk ferment in the torrid zone Mother, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, mesophilic Sphingobacterium, the false silk in the torrid zone Yeast, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, mesophilic Sphingobacterium, the false silk ferment in the torrid zone Mother, bacillus firmus and Lactobacillus plantarum, or, bacillus megaterium, pseudomonas, mesophilic Sphingobacterium, the false silk in the torrid zone Yeast, bacillus firmus and Lactobacillus plantarum are cultivated respectively；And obtain the respective culture of described bacterial strain, and will Described culture mixes according to combinations thereof.

In one embodiment, by described bacillus subtilis, bacillus megaterium, pseudomonas, mesophilic sphingol Bacillus, candida tropicalis, bacillus firmus and Lactobacillus plantarum are cultivated respectively；And obtain the respective of described bacterial strain Culture, and described culture is mixed according to combinations thereof.

In one embodiment, by bacillus subtilis, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium Cultivate respectively, it is thus achieved that the respective culture of described bacterial strain, and by described culture according to described bacillus subtilis, huge Bacterium anthracoides, pseudomonas and mesophilic Sphingobacterium, bacillus subtilis, bacillus megaterium and pseudomonas, hay Bacillus cereus, bacillus megaterium and mesophilic Sphingobacterium, bacillus subtilis, pseudomonas and mesophilic Sphingobacterium, Or, the combination of bacillus megaterium, pseudomonas and mesophilic Sphingobacterium carries out being mixed to get described composite bacteria agent capable A；By heat Band candida mycoderma, bacillus firmus and Lactobacillus plantarum are cultivated respectively, it is thus achieved that the respective culture of described bacterial strain, and Carry out being mixed to get described composite bacteria agent capable B according to combinations thereof by described culture；Described composite bacteria agent capable include composite bacteria agent capable A and Composite bacteria agent capable B.

On the other hand, the application further relates to process or the method for aid in treatment mud, including:

Described compositions or described composite bacteria agent capable are inoculated in pending mud carry out processing at least 5 days, at least 10 days, At least 15 days, at least 20 days, at least 25 days, at least 30 days, at least 35 days, at least 40 days or at least 45 days.

In one embodiment, described compositions or described composite bacteria agent capable are inoculated in pending mud process At least 30 days.

In one embodiment, described compositions or described composite bacteria agent capable are relative to the inoculum concentration of described pending mud It is at least 0.1%, at least 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w.

In one embodiment, described compositions A or described composite bacteria agent capable A are inoculated in pending mud and locate Manage at least 5 days, at least 10 days or at least 15 days, preferably at least 10 days, obtain first fermentation material；Then by described compositions B or described composite bacteria agent capable B is inoculated in first fermentation material and carries out processing at least 5 days, at least 10 days, at least 15 days, and at least 20 My god, at least 25 days or at least 30 days, preferably at least 20 days.

In some embodiments, the process time has all been affected by season, temperature difference.Summer is by described compositions A Or described composite bacteria agent capable A is inoculated in pending mud and carries out being processed as at least 5～7 days, then by described compositions B or described Composite bacteria agent capable B is inoculated in first fermentation material and carries out being processed as 7～12 days；Spring and autumn are by described compositions A or described Composite bacteria agent capable A is inoculated in pending mud and carries out being processed as 7～10 days, then by described compositions B or described composite bacteria agent capable B It is inoculated in first fermentation material and carries out being processed as 12～15 days；Described compositions A or described composite bacteria agent capable A are inoculated into by winter Pending mud is carried out be processed as 10～15 days, then described compositions B or described composite bacteria agent capable B are inoculated into first fermentation Material is carried out be processed as 15～20 days.

In some embodiments, described compositions A or described composite bacteria agent capable A relative to the inoculum concentration of pending mud are At least 0.1%, at least 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w, it is therefore preferable to At least 0.5%, at least 1%, at least 2% or at least 3%w/w；Described compositions B or described composite bacteria agent capable B send out relative to first The inoculum concentration of ferment material is at least 0.1%, at least 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w, it is therefore preferable at least 0.5%, at least 1%, at least 2% or at least 3%w/w.

In some embodiments, described pending mud can add adjuvant, such as: wood flour, rice husk or straw Ground product (e.g., corn straw or wheat stalk)；The addition of described adjuvant is the 5～15% of described pending sludge volume, Such as 10%；The particle diameter of described adjuvant is 5～15mm.

On the other hand, the application further relates to soil conditioner, obtains after comprising the described described pending mud of process Solid content.

In one embodiment, in the solid content obtained after using the process of colony counting method mensuration in composite bacteria agent capable used Bacterial strain living bacteria count >=1 × 10⁸cfu/g。

In some embodiments, the water content of described soil conditioner is≤30% ,≤25% ,≤20% ,≤15%, It is preferably≤20%.

In some embodiments, the particle diameter of described soil conditioner be≤1mm ,≤2.5mm ,≤3mm ,≤5mm ,≤ 7.5mm or≤10mm, it is therefore preferable to≤2.5mm.

In one embodiment, described soil conditioner be prepared as will be after the described described pending mud of process To solid content pulverize, grinding and sieving, obtain the granule of required size, be soil conditioner.

On the other hand, the application further relates to the method for soil improvement, including:

The solid content or the described soil conditioner that obtain after processing described pending mud by method disclosed herein add Enter and carry out processing at least 5 days, at least 10 days, at least 15 days, at least 20 days, at least 25 days or at least 30 in soil to be improved My god.

In one embodiment, the solid content obtained after the described described pending mud of process or described soil are changed Good dose joins and carries out in soil to be improved processing at least 20 days.

In some embodiments, the solid content obtained after the described described pending mud of process or described soil improvement Agent is at least 5, at least 10, at least 15 or at least 20kg/m relative to the addition of soil to be improved³。

In one embodiment, the solid content obtained after the described described pending mud of process or described soil improvement Agent is at least 10kg/m relative to the addition of soil to be improved³。

Describe in detail

There is provided defined below and method in order to preferably to define the application and to instruct this area general in the application puts into practice Logical technical staff.Unless otherwise mentioned, term understands according to the common usage of person of ordinary skill in the relevant.Cited herein All patent documentations, scientific paper and other public publications, full content therein is incorporated herein by reference.

Definition

Term as used herein " compositions ", refers to comprise the mixture of two or more microorganism fungus kind, or It is made up of described microorganism fungus kind.If it is required, the described bacterial strain that is conducive to that described compositions can also include other preserves Material, such as, culture medium, trace element, vitamin, aminoacid, meat soup etc..

Term as used herein " composite bacteria agent capable ", refers to comprise two or more and the microorganism fungus kind of mutual the most not antagonism The microorganism formulation made, described composite bacteria agent capable is with described strain as active component, it is also possible to additionally comprise carrier or excipient, Can also comprise other be conducive to described bacterial strain preserve material, such as, culture medium, trace element, vitamin, aminoacid, Meat soup etc..In one embodiment, described microbial inoculum compatibility is reasonable, have the advantages such as synergism.

Term as used herein " culture ", refers to be trained specific by microorganism fungus kind under specific process conditions control Supporting the microbial product formed the most afterwards on base, it mainly comprises microorganism fungus kind cell mass, microorganism after by fermentation Bacterium cell extra-metabolite and culture medium.

Term as used herein " mud ", refers at hydrostatic and the particulate that deposits and contain organic matter slowly in flowing water environment Soil, its natural moisture content is more than liquid limit, and natural void ratio is more than 1.5.

Term as used herein " broken glue ", refers to destroy the stability of colloid for making colloidal solid sedimentation, promotes colloid Granule contacts with each other, and becomes bigger granule.

Term as used herein " organic ", the organic compound of carbon containing, including plant and animal residue, microorganism and its point Solve the compound of synthesis.

Term as used herein " solid content ", refers to destroy the colloform texture of mud, converts the macromole in mud organic Thing and heavy metal, the material of mummification after making the moisture in mud evaporate.

Term as used herein " soil conditioner ", refers to the bad quality for soil and structure, takes corresponding thing Reason, biological or chemical measure, improve soil property, increase soil fertility, and increases crop yield, and improves human survival soil The process of environment.

Terms used herein " soil weight ", after referring to that the soil (including the hole of grogs and intergranular) of a constant volume is dried The ratio of weight and co-content water weight.

Term as used herein " soil porosity ", refers to thickness grogs set variously-shaped in soil and is arranged in solid Phase skeleton, skeletal internal has width and variform hole, constitutes complicated pore system, and whole void contents are held with the soil body Long-pending percentage rate.

Term as used herein " aeration porosity ", refers under certain flow of water or Water Content Conditions, unit soil total volume The void content that middle air accounts for.

Term as used herein " water capacity ", refer to certain state soil opposing gravity institute energy sticking flood Amount, represents accounting for the percent of soil volume, and for comparing the water holding capacity of soil, the physicochemical properties of soil particle are special Being that granular size, structure and the content of organic matter are all relevant with this numerical value, the water capacity under naturalness is referred to as field water holding Amount, is the higher limit determining the effective water of plant.

Term as used herein " particle diameter ", refers to when certain physical characteristic of tested granule or physical behavio(u)r and a certain diameter Homogenous spheres (or combination) the most close time, just using the diameter (or combination) of this spheroid as tested granule equivalent grain size (or Particle size distribution).

Detailed description of the invention

In some embodiments, total living bacteria count >=1 in described compositions or in described composite bacteria agent capable × 10⁸cfu/g；

It is highly preferred that total living bacteria count >=1 × 10 in described compositions A or composite bacteria agent capable A⁸cfu/g；Described combination Total living bacteria count >=1 × 10 in thing B or composite bacteria agent capable B⁸cfu/g。

In some embodiments, in terms of the dry weight of described strain culture, wherein said compositions A or described compound bacteria In agent, bacillus subtilis culture, bacillus megaterium culture, pseudomonas culture, mesophilic Sphingobacterium are cultivated In thing, candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture at least six kinds or seven kinds Weight portion is:

Bacillus subtilis culture 2～5

Bacillus megaterium culture 1～3

Pseudomonas culture 2～4

Mesophilic Sphingobacterium culture 2～5

Candida tropicalis culture 3～5

Bacillus firmus culture 1～3

Lactobacillus plantarum culture 1～3,

Optionally, described culture comprises bacterial strain and carrier；

Optionally, the living bacteria count of bacillus subtilis described in described bacillus subtilis culture be >=1 × 10⁸cfu/g；Described in described bacillus megaterium culture, the living bacteria count of bacillus megaterium is >=1 × 10⁸cfu/g； Described in described pseudomonas culture, the living bacteria count of pseudomonas is >=1 × 10⁸cfu/g；Described mesophilic sphingol bar The living bacteria count of mesophilic Sphingobacterium described in bacterium culture is >=1 × 10⁸cfu/g；Described candida tropicalis is cultivated The living bacteria count of candida tropicalis described in thing is >=1 × 10⁷cfu/g；Described in described bacillus firmus culture The living bacteria count of bacillus firmus is >=1 × 10⁸cfu/g；Lactobacillus plantarum described in described Lactobacillus plantarum culture Living bacteria count be >=1 × 10⁸cfu/g；

Optionally, described carrier comprises solid medium, semisolid culturemedium or fluid medium.

In one embodiment, bacillus subtilis culture in described compositions or in described composite bacteria agent capable, huge Bacillus culture, pseudomonas culture, candida tropicalis culture, bacillus firmus culture and plant breast bar Dry weight between bacterium culture is than for 1:1:1:1:1:1.

In one embodiment, bacillus subtilis culture in described compositions or in described composite bacteria agent capable, huge Bacillus culture, mesophilic Sphingobacterium culture, candida tropicalis culture, bacillus firmus culture and plant Dry weight between thing culture of L is than for 1:1:1:1:1:1.

In one embodiment, bacillus subtilis culture in described compositions or in described composite bacteria agent capable, false single Born of the same parents' bacterium culture, mesophilic Sphingobacterium culture, candida tropicalis culture, bacillus firmus culture and plant breast Dry weight between alphacterium culture is than for 1:1:1:1:1:1.

In one embodiment, bacillus megaterium culture in described compositions or in described composite bacteria agent capable, false single Born of the same parents' bacterium culture, mesophilic Sphingobacterium culture, candida tropicalis culture, bacillus firmus culture and plant breast Dry weight between alphacterium culture is than for 1:1:1:1:1:1.

In one embodiment, dry weight between seven kinds of strain cultures in described compositions or in described composite bacteria agent capable Ratio is 1:1:1:1:1:1:1.

In one embodiment, bacillus subtilis culture, huge spore in described compositions A or composite bacteria agent capable A Dry weight between alphacterium culture, pseudomonas culture and mesophilic Sphingobacterium culture is than for 1:1:1:1；Described combination In thing B or composite bacteria agent capable B between candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture Dry weight is than for 1:1:1.

In one embodiment, bacillus subtilis culture, huge spore in described compositions A or composite bacteria agent capable A Dry weight between alphacterium culture and pseudomonas culture is than for 1:1:1；In described compositions B or composite bacteria agent capable B, the torrid zone is false Silk yeast culture, dry weight between bacillus firmus culture and Lactobacillus plantarum culture ratio is for 1:1:1.

In one embodiment, bacillus subtilis culture, huge spore in described compositions A or composite bacteria agent capable A Dry weight between alphacterium culture and mesophilic Sphingobacterium culture is than for 1:1:1；In described compositions B or composite bacteria agent capable B Dry weight between candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, bacillus subtilis culture, pseudomonas in described compositions A or composite bacteria agent capable A Dry weight between culture and mesophilic Sphingobacterium culture is than for 1:1:1；The torrid zone in described compositions B or composite bacteria agent capable B Dry weight between candida mycoderma culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, bacillus megaterium culture, pseudomonas in described compositions A or composite bacteria agent capable A Dry weight between culture and mesophilic Sphingobacterium culture is than for 1:1:1；The torrid zone in described compositions B or composite bacteria agent capable B Dry weight between candida mycoderma culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, by described bacillus subtilis, bacillus megaterium, pseudomonas, the false silk ferment in the torrid zone Mother, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, mesophilic Sphingobacterium, the false silk in the torrid zone Yeast, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, mesophilic Sphingobacterium, the false silk ferment in the torrid zone Mother, bacillus firmus and Lactobacillus plantarum, or bacillus megaterium, pseudomonas, mesophilic Sphingobacterium, the false silk ferment in the torrid zone Mother, bacillus firmus and Lactobacillus plantarum are cultivated respectively；And obtain the respective culture of described bacterial strain, and by institute State culture to mix according to combinations thereof, obtain described composite bacteria agent capable.

In one embodiment, by described bacillus subtilis, bacillus megaterium, pseudomonas, mesophilic sphingol Bacillus, candida tropicalis, bacillus firmus and Lactobacillus plantarum are cultivated respectively；And obtain the respective of described bacterial strain Culture, and described culture is mixed according to combinations thereof, obtains described composite bacteria agent capable.

In some embodiments, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture during mixing, bacillus megaterium culture, pseudomonas culture, addicted to In temperature Sphingobacterium culture, candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture The weight portion of at least six kinds or seven kinds is:

Bacillus subtilis culture 2～5

Bacillus megaterium culture 1～3

Pseudomonas culture 2～4

Mesophilic Sphingobacterium culture 2～5

Candida tropicalis culture 3～5

Bacillus firmus culture 1～3

Lactobacillus plantarum culture 1～3；

Optionally, described culture comprises bacterial strain and carrier；

Optionally, total living bacteria count >=1 × 10 in described composite bacteria agent capable⁸cfu/g；

It is highly preferred that total living bacteria count >=1 × 10 in described composite bacteria agent capable A⁸cfu/g；In described composite bacteria agent capable B Total living bacteria count >=1 × 10⁸cfu/g；

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture, bacillus megaterium culture, pseudomonas culture, heat during mixing With the dry weight between candida mycoderma culture, bacillus firmus culture and Lactobacillus plantarum culture than for 1:1:1:1:1: 1。

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, and during mixing, bacillus subtilis culture, bacillus megaterium culture, mesophilic Sphingobacterium are cultivated Dry weight between thing, candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1: 1:1:1:1。

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture during mixing, pseudomonas culture, mesophilic Sphingobacterium culture, Dry weight between candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1:1: 1:1。

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus megaterium culture during mixing, pseudomonas culture, mesophilic Sphingobacterium culture, Dry weight between candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1:1: 1:1。

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, and during mixing, dry weight between seven kinds of strain cultures is than for 1:1:1:1:1:1:1.

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture, bacillus megaterium culture, vacation in described composite bacteria agent capable A during mixing Dry weight between Pseudomonas culture and mesophilic Sphingobacterium culture is than for 1:1:1:1；In described composite bacteria agent capable B, the torrid zone is false Silk yeast culture, dry weight between bacillus firmus culture and Lactobacillus plantarum culture ratio is for 1:1:1.

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture, bacillus megaterium culture and vacation in described composite bacteria agent capable A during mixing Dry weight between Pseudomonas culture is than for 1:1:1；Candida tropicalis culture, strong spore bar in described composite bacteria agent capable B Dry weight between bacterium culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture in described composite bacteria agent capable A during mixing, bacillus megaterium culture and addicted to Dry weight ratio between temperature Sphingobacterium culture is for 1:1:1；Candida tropicalis culture in described composite bacteria agent capable B, strong Dry weight between bacillus culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus subtilis culture, pseudomonas culture and mesophilic sheath in described composite bacteria agent capable A during mixing Dry weight between ammonia alcohol alphacterium culture is than for 1:1:1；Candida tropicalis culture, strong spore in described composite bacteria agent capable B Dry weight between alphacterium culture and Lactobacillus plantarum culture is than for 1:1:1.

In one embodiment, in the preparation method of described composite bacteria agent capable, with the respective culture of described bacterial strain Dry weight meter mixes, bacillus megaterium culture, pseudomonas culture and mesophilic sheath in described composite bacteria agent capable A during mixing Dry weight between ammonia alcohol alphacterium culture is than for 1:1:1；Candida tropicalis culture, strong spore in described composite bacteria agent capable B Dry weight between alphacterium culture and Lactobacillus plantarum culture is than for 1:1:1.

Strain used herein is known strain, can be obtained by routine screening, commercial means or other approach.

In one embodiment, can by be either commercially available strain as herein described, such as bacillus subtilis can To be bacillus subtilis (Bacillus subtilis), selectively, can be from Zi Rui bio tech ltd, Xi'an ( Location: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtain above-mentioned bacterial strains.

In one embodiment, can by be either commercially available strain as herein described, such as bacillus megaterium can To be bacillus megaterium (Bacillus megaterium), selectively, can be from Zi Rui bio tech ltd, Xi'an (address: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtains above-mentioned bacterial strains.

In one embodiment, can be able to be by being either commercially available strain as herein described, such as pseudomonas Pseudomonas aeruginosa (Pseudomonas aeruginosa), selectively, can be from Zi Rui bio tech ltd, Xi'an ( Location: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtain above-mentioned bacterial strains.

In one embodiment, can be by being either commercially available strain as herein described, the most mesophilic Sphingobacterium Can be mesophilic Sphingobacterium (Sphingobacterium thalpophilum), selectively, can be from the Xi'an auspicious biology of purple Science and Technology Ltd. (address: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtains above-mentioned bacterial strains.

In one embodiment, can by be either commercially available strain as herein described, such as candida tropicalis can To be candida tropicalis (Candida tropicalis), selectively, can be from Zi Rui bio tech ltd, Xi'an ( Location: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtain above-mentioned bacterial strains.

In one embodiment, can by be either commercially available strain as herein described, such as bacillus firmus can To be bacillus firmus (Bacillus firmus), selectively, can from Zi Rui bio tech ltd, Xi'an (address: Eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtain above-mentioned bacterial strains.

In one embodiment, can be permissible by being either commercially available strain as herein described, such as Lactobacillus plantarum It is Lactobacillus plantarum (Lactobacillus plantarum), selectively, can be from Zi Rui bio tech ltd, Xi'an (address: eastern section, Mei Wu main road, new district, riverfront, Mei County, Shaanxi Province, postcode: 722300) obtains above-mentioned bacterial strains.

In some embodiments, the solid medium of the solid culture for cultivating described strain can be that wheat bran is cultivated Base, potato culture, Testa oryzae culture medium, soybean cake powder culture medium or peanut cake powder culture medium.

In some embodiments, in the fluid medium of the liquid culture cultivating described strain, the false silk ferment in the torrid zone Mother can use PDA fluid medium, malt extract medium or bean sprouts medium；Bacillus subtilis, bacillus megaterium, Mesophilic Sphingobacterium, bacillus firmus and Pseudomonas aeruginosa can use beef extract-peptone fluid medium, LB to train Support base or broth bouillon；Lactobacillus plantarum can use MRS fluid medium or Semen Maydis powder fluid medium.

In some embodiments, the semisolid culturemedium of the Semi-solid cell culture for cultivating described strain can be at liquid Culture medium adds a small amount of coagulator (agar such as 0.2%～0.5%) be prepared from.

Culture mainly by microorganism fungus kind cell, microorganism fungus kind extracellular metabolic product (such as enzyme, polysaccharide, lipid, Organic acid etc.) and fermentation after culture medium.

In one embodiment, the preparation method of composite bacteria agent capable includes at least one method following or step:

(1) test tube slant: by bacillus subtilis, bacillus megaterium, Pseudomonas aeruginosa, mesophilic Sphingobacterium and Bacillus firmus, respectively in beef extract-peptone solid medium, is cultivated at least 24 hours, is obtained respectively for 32 DEG C～35 DEG C Bacillus subtilis test tube strains, bacillus megaterium test tube strains, Pseudomonas aeruginosa test tube strains, mesophilic Sphingobacterium Test tube strains and bacillus firmus test tube strains；Candida tropicalis, in PDA solid medium, is cultivated extremely for 32 DEG C～35 DEG C Few 24 hours, it is thus achieved that candida tropicalis test tube strains；Lactobacillus plantarum in MRS solid medium, 35 DEG C～40 DEG C cultivations At least 24 hours, it is thus achieved that Lactobacillus plantarum test tube strains.

(2) shake-flask culture: by bacillus subtilis test tube strains, bacillus megaterium test tube strains, Aerugo in step (1) Pseudomonas test tube strains, mesophilic Sphingobacterium test tube strains and bacillus firmus test tube strains are respectively connected to Carnis Bovis seu Bubali cream egg In white peptone fluid medium, 30 DEG C～37 DEG C carry out shake-flask culture 24～48 hours, obtaining the training of bacillus subtilis shaking flask respectively Bacteria liquid, bacillus megaterium shake-flask culture bacterium solution, Pseudomonas aeruginosa shake-flask culture bacterium solution, mesophilic Sphingobacterium shaking flask are trained Bacteria liquid and bacillus firmus shake-flask culture bacterium solution；Candida tropicalis test tube strains in step (1) is accessed the training of PDA liquid Supporting in base, 28 DEG C～37 DEG C carry out shake-flask culture 24～48 hours, it is thus achieved that candida tropicalis shake-flask culture bacterium solution；By step (1) in, Lactobacillus plantarum test tube strains accesses in MRS fluid medium, and 30 DEG C～37 DEG C carry out shake-flask culture 24～48 hours, Obtain Lactobacillus plantarum shake-flask culture bacterium solution.

(3) seed tank culture: by bacillus subtilis shake-flask culture bacterium solution, the training of bacillus megaterium shaking flask in step (2) Bacteria liquid, Pseudomonas aeruginosa shake-flask culture bacterium solution, mesophilic Sphingobacterium shake-flask culture bacterium solution and bacillus firmus shaking flask Cultivating bacterium solution to be respectively connected in beef extract-peptone fluid medium, 30 DEG C～37 DEG C carry out seed tank culture 24～48 hours, Obtain bacillus subtilis seed liquor, bacillus megaterium seed liquor, Pseudomonas aeruginosa seed liquor, mesophilic sphingol bar respectively Bacterium seed liquor and bacillus firmus seed liquor；Candida tropicalis shake-flask culture bacterium solution in step (2) is accessed Glucose Liquid In body culture medium, 28 DEG C～37 DEG C carry out seed tank culture 24～48 hours, it is thus achieved that candida tropicalis seed liquor；By step (2) in, Lactobacillus plantarum shake-flask culture bacterium solution accesses in Semen Maydis powder fluid medium, 30 DEG C～37 DEG C carry out shake-flask culture 24～ 48 hours, it is thus achieved that Lactobacillus plantarum seed liquor.

(4) fermentation tank: by bacillus subtilis seed liquor, bacillus megaterium seed liquor, P. aeruginosa in step (3) Bacterium seed liquor, mesophilic Sphingobacterium seed liquor and bacillus firmus seed liquor are respectively connected to beef extract-peptone liquid culture In base, 30 DEG C～37 DEG C carry out seed tank culture 24～48 hours, obtaining fermentation of bacillus subtilis liquid, huge spore bar respectively Fermented liquid, P. aeruginosa fermented liquid, mesophilic Sphingobacterium fermentation liquid and bacillus firmus fermentation liquid；By step (3), during in, candida tropicalis seed liquor accesses dextrose broth, 28 DEG C～37 DEG C carry out seed tank culture 24～48 Hour, it is thus achieved that candida tropicalis fermentation liquid；Lactobacillus plantarum seed liquor in step (3) is accessed Semen Maydis powder fluid medium In, 30 DEG C～37 DEG C carry out shake-flask culture 24～48 hours, it is thus achieved that Lactobacillus plantarum fermentation liquid.Bacterial strain in each bacterial strain fermentation liquor Living bacteria count is all >=1 × 10⁸cfu/mL

(5) solid culture: by fermentation of bacillus subtilis bacterium solution, bacillus megaterium zymocyte liquid, Aerugo in step (4) Pseudomonas zymocyte liquid, mesophilic Sphingobacterium zymocyte liquid, bacillus firmus zymocyte liquid and candida tropicalis send out Yeast-like fungi liquid according to the ratio of 10%～30%v/w be added separately to bran mass (consisting of of percentage by weight: wheat bran (as Testa Tritici): bean cake: powdered rice hulls=6: 2: 2) in carry out solid culture, solid culture during carry out turning, it is ensured that temperature of charge Less than 40 DEG C, obtain bacillus subtilis solid culture, bacillus megaterium solid culture, Pseudomonas aeruginosa respectively solid Body culture, mesophilic Sphingobacterium solid culture, bacillus firmus solid culture and the training of candida tropicalis solid Support thing；Lactobacillus plantarum zymocyte liquid in step (4) is comprehensively trained to Semen Maydis powder according to the ratio addition of 10%～30%v/w Support and base (consisting of of percentage by weight: Semen Maydis powder: wheat bran (such as Testa Tritici): bean cake=7: 2: 1) carries out semi-solid anaerobism Cultivate, it is thus achieved that Lactobacillus plantarum solid culture；Effective viable bacteria of bacillus subtilis in bacillus subtilis solid culture Number is for >=1 × 10⁸cfu/g；In bacillus megaterium solid culture the living bacteria count of bacillus megaterium be >=1 × 10⁸cfu/g；In Pseudomonas aeruginosa solid culture, the living bacteria count of Pseudomonas aeruginosa is >=1 × 10⁸cfu/g；Mesophilic In Sphingobacterium solid culture, the living bacteria count of mesophilic Sphingobacterium is >=1 × 10⁸cfu/g；Candida tropicalis In solid culture, the living bacteria count of candida tropicalis is >=1 × 10⁷cfu/g；In bacillus firmus solid culture The living bacteria count of bacillus firmus is >=1 × 10⁸cfu/g；In Lactobacillus plantarum solid culture, Lactobacillus plantarum has Effect viable count is >=1 × 10⁸cfu/g。

Containing B. subtilis cell group, bacillus subtilis metabolite in bacillus subtilis solid culture Bran mass after (such as protease, lipase, cellulase, α-amylase, lactic acid etc.) and fermentation；Bacillus megaterium Containing bacillus megaterium cell mass, bacillus megaterium metabolite (such as glucose isomerase, albumen in solid culture Enzyme, cellulase etc.) and fermentation after bran mass；Pseudomonas aeruginosa solid culture contains Pseudomonas aeruginosa thin After born of the same parents group, Pseudomonas aeruginosa metabolite (such as chitinase, lipase, auxin, siderophore, antibiotic etc.) and fermentation Bran mass；Containing mesophilic Sphingobacterium cell mass, mesophilic sphingol bar in mesophilic Sphingobacterium solid culture Bran mass after bacterium metabolite (such as biomass flocculant) and fermentation；Bacillus firmus solid culture contains Wheat bran after bacillus firmus cell mass, bacillus firmus metabolite (such as amylase, protease) and fermentation is cultivated Base；Containing Candida tropicalis cells group, candida tropicalis metabolite (such as fibre in candida tropicalis solid culture Dimension element enzyme, pectase and 4-oxo deacidification enzyme etc.) and bran mass after fermenting；Lactobacillus plantarum solid culture contains Semen Maydis powder synthetic medium after Lactobacillus plantarum cell mass, Lactobacillus plantarum metabolite (such as lactic acid, acetic acid) and fermentation.

(6) preparation of solid fungicide: by above-mentioned bacillus subtilis solid culture, bacillus megaterium solid culture Thing, Pseudomonas aeruginosa solid culture, mesophilic Sphingobacterium solid culture, bacillus firmus solid culture, heat Band candida mycoderma solid culture and Lactobacillus plantarum solid culture are dried respectively, and being dried can be vacuum drying or fluidisation Bed be dried respectively obtain bacillus subtilis solid fungicide, bacillus megaterium solid fungicide, Pseudomonas aeruginosa solid fungicide, Mesophilic Sphingobacterium solid fungicide, bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum Solid fungicide；In bacillus subtilis solid fungicide, the living bacteria count of bacillus subtilis is >=1 × 10⁸cfu/g；Huge bud In spore bacillus solid fungicide, the living bacteria count of bacillus megaterium is >=1 × 10⁸cfu/g；Pseudomonas aeruginosa solid fungicide The living bacteria count of middle Pseudomonas aeruginosa is >=1 × 10⁸cfu/g；Mesophilic sphingol in mesophilic Sphingobacterium solid fungicide The living bacteria count of bacillus is >=1 × 10⁸cfu/g；Effective viable bacteria of candida tropicalis in candida tropicalis solid fungicide Number is for >=1 × 10⁷cfu/g；In bacillus firmus solid fungicide, the living bacteria count of bacillus firmus is >=1 × 10⁸cfu/ g；In Lactobacillus plantarum solid fungicide, the living bacteria count of Lactobacillus plantarum is >=1 × 10⁸cfu/g。

(7) prepared by composite bacteria agent capable: composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B.

In one embodiment, by bacillus subtilis solid fungicide, bacillus megaterium solid fungicide, Aerugo vacation list Born of the same parents' bacterium solid fungicide and mesophilic Sphingobacterium solid fungicide mix than 1:1:1:1 according to dry weight, obtain composite bacteria agent capable A, Total living bacteria count >=1 × 10 in composite bacteria agent capable A⁸cfu/g.By solid to bacillus firmus solid fungicide, candida tropicalis Body microbial inoculum and Lactobacillus plantarum solid fungicide mix than 1:1:1 according to dry weight, obtain composite bacteria agent capable B, in composite bacteria agent capable B Total living bacteria count >=1 × 10⁸cfu/g。

In one embodiment, bacillus subtilis solid fungicide, bacillus megaterium solid fungicide and Aerugo is false Zymomonas mobilis solid fungicide mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable A, the total living bacteria count in composite bacteria agent capable A ≥1×10⁸cfu/g.By bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum solid fungicide Mix than 1:1:1 according to dry weight, obtain composite bacteria agent capable B, total living bacteria count >=1 × 10 in composite bacteria agent capable B⁸cfu/g。

In one embodiment, by bacillus subtilis solid fungicide, bacillus megaterium solid fungicide and mesophilic sheath Ammonia alcohol bacillus solid fungicide mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable A, the total effectively viable bacteria in composite bacteria agent capable A Number >=1 × 10⁸cfu/g.By bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum solid bacterium Agent mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable B, total living bacteria count >=1 × 10 in composite bacteria agent capable B⁸cfu/ g。

In one embodiment, by bacillus subtilis solid fungicide, Pseudomonas aeruginosa solid fungicide and mesophilic sheath Ammonia alcohol bacillus solid fungicide mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable A, the total effectively viable bacteria in composite bacteria agent capable A Number >=1 × 10⁸cfu/g.By bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum solid bacterium Agent mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable B, total living bacteria count >=1 × 10 in composite bacteria agent capable B⁸cfu/ g。

In one embodiment, by bacillus megaterium solid fungicide, Pseudomonas aeruginosa solid fungicide and mesophilic sheath Ammonia alcohol bacillus solid fungicide mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable A, the total effectively viable bacteria in composite bacteria agent capable A Number >=1 × 10⁸cfu/g.By bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum solid bacterium Agent mixes than 1:1:1 according to dry weight, obtains composite bacteria agent capable B, total living bacteria count >=1 × 10 in composite bacteria agent capable B⁸cfu/ g。

In some embodiments, the process time has all been affected by Various Seasonal, temperature.Summer is by described compositions A Or described composite bacteria agent capable A is inoculated in pending mud and carries out being processed as at least 5～7 days, then by described compositions B or described Composite bacteria agent capable B is inoculated in first fermentation material and carries out being processed as 7～12 days；Spring and autumn are by described compositions A or described Composite bacteria agent capable A is inoculated in pending mud and carries out being processed as 7～10 days, then by described compositions B or described composite bacteria agent capable B It is inoculated in first fermentation material and carries out being processed as 12～15 days；Described compositions A or described composite bacteria agent capable A are inoculated into by winter Carrying out in pending mud being processed as 10～15 days, then by described compositions B, or described composite bacteria agent capable B is inoculated into first sending out Ferment material is carried out be processed as 15～20 days.

In some embodiments, pending silt can be river course, lake and blowdown channel just desilting silt out at mud Mud, it is also possible to for placing the mud after a period of time.In mud, water content is high, containing organic matter, and harmful heavy metal and evil Foul smell taste.Need when mud is processed to reach dehydration, broken glue, deodorization and the purpose become thoroughly decomposed.

In one embodiment, by described compositions A or described composite bacteria agent capable A according to pending mud weight 0.5%, 1%, 2% or 3% be inoculated in pending mud, and add pending sludge volume 10% adjuvant (such as wood Bits or powder of straw mince) regulation moisture so that the water content in mixed material is less than 70%, when temperature being higher than 45 DEG C, often Used turner to carry out turning every 12 hours, reach crushing material, lower the temperature and lead to the purpose of oxygen, carry out processing at least 10 days, To first fermentation material, the water content of first fermentation material is less than 50%；Then by described compositions B or described composite bacteria agent capable B Being inoculated in first fermentation material and carry out ferment in second time, turning in every 2～3 days once, processes at least 20 days, the silt after being processed Mud, the mud water content after process is less than 30%.

In some embodiments, composite bacteria agent capable is inoculated in pending mud, by machinery or manually by compound bacteria Agent and the mixing of pending mud.

In one embodiment, obtain after using the described pending mud of process described in colony counting method mensuration consolidates Bacterial strain living bacteria count >=1 × 10 in composite bacteria agent capable used in shape thing⁸cfu/g。

Said method is processed the solid content obtained after described pending mud and carries out physical and chemical index qualification, as water content, PH value, the content of organic matter, content of beary metal (such as hydrargyrum, lead, cadmium, copper, zinc, chromium or arsenic).

Moisture content is measured according to the gravimetric method in CJ/T221；PH value is carried out according to the glass electrode method in CJ/T221 Measure；The content of organic matter is measured according to the gravimetric method in CJ/T221；Mercury content is according to the Atomic Absorption in GB/T17136 Spectrophotography is measured；Lead content is measured according to the atomic absorption spectrophotometry in CJ/T221；Cadmium content according to Atomic absorption spectrophotometry in GB/T17141 is measured；Copper content is according to the Flame Atomic Absorption Spectrometry light splitting in CJ/T221 Photometry is measured；Zn content is measured according to the atomic absorption spectrophotometry in GB/T17138；Chromium content root It is measured according to the atomic absorption spectrophotometry in GB/T17136；Arsenic content is according to the diethyl two in GB/T17134 Thiocarbamic acid silver spectrophotography is measured.

In some embodiments, the water content of described soil conditioner be≤30% ,≤25% ,≤20% ,≤15%, ≤ 10% or≤5%, it is therefore preferable to≤20%.

In one embodiment, described soil conditioner be prepared as can be by after the described described pending mud of process The solid content obtained is pulverized, grinding and sieving, obtains the granule of required size, is soil conditioner.

The soil weight uses core cutter method to be measured；Soil porosity uses core cutter method to be measured；Soil pH uses glass Glass electrode method is measured；The soil organism uses potassium bichromate titrimetric method to be measured.

Aeration porosity=(the 1-soil weight/soil bulk density-soil weight × water capacity) × 100% is public Formulas I；

Wherein, water capacity is surveyed according to the mensuration core cutter method of NY/T 1121.22-2010 water-retaining quantity among field of soil Fixed.

In one embodiment, the aeration porosity=(the 1-soil weight/soil bulk density-soil weight × soil Earth water-holding capacity) × 100% formula I；

Wherein, soil bulk density is 2.65g/cm³, water capacity is held according to NY/T1121.22-2010 soil field The mensuration core cutter method of the water yield is measured.

Therefore, the application provide for processing compositions or the composite bacteria agent capable of mud, there is at least one advantage following:

(1), in the compositions of the application or composite bacteria agent capable, each bacterial strain adapts to natural environment, is inoculated in mud, selected Educate bacterial strain can survive, carry out metabolic activity and mud is fermented thus reach silt harmless purpose.

(2) in the compositions of the application or composite bacteria agent capable, each bacterial strain energy fast-growth is bred, in being inoculated into mud 1～3 My god, the living bacteria count of each bacterial strain i.e. can reach 1 × 10⁸Cfu/g, it is possible to reach quickly to process mud purpose.

(3), in the compositions of the application or composite bacteria agent capable, each bacterial strain adapts to mud environment, its metabolite, such as breast Acid, amylase, protease, lipase etc. can suppress the growth of other miscellaneous bacterias, are more beneficial for the process of mud.

(4) in the compositions of the application or composite bacteria agent capable, each bacterial strain reasonable mixture ratio, it is possible to play respective degraded advantage.

(5) in the compositions of the application or composite bacteria agent capable, each bacterial strain can synergism, degradation speed speeds, and shortens field Take the cycle, save the area.

(6), in the compositions of the application or composite bacteria agent capable, each bacterial strain can make material be brought rapidly up, high temperature (more than 55 DEG C) Persistent period is long, makes rivers and lakes mud be rapidly achieved dehydration, broken glue, deodorizing effect.

(7) rich in organic and probiotic bacteria during the composite bacteria agent capable of the application processes the solid content that rivers and lakes mud obtains.

(8) solid content that the composite bacteria agent capable process rivers and lakes mud of the application obtains can change as soil conditioner Kind soil compaction situation, increase soil fertility, stimulation and coordinate plant growth.

(9) this application provides a kind of efficient, innoxious, skill of lake silt of harnessing a river that low cost, generalization are strong Art method, utilizes complex microbial community cleverly, degrades the pollution in the mud of rivers and lakes thoroughly by ferment in second time Thing, reaches the purpose of rivers and lakes silt harmless.

In some embodiments, compositions or the composite bacteria agent capable for processing mud that the application provides, it is thus achieved that following At least one effect:

(1) after using the compositions of the application or composite bacteria agent capable to process mud, relative to matched group, aqueous in process group Rate reduces at least 20%, and concrete data see embodiment.

(2) after using compositions or the composite bacteria agent capable process mud of the application, relative to matched group, each huge sum of money in process group Belonging to content all to significantly reduce, each content of beary metal reduces at least 25%, and concrete data see embodiment.Wherein, relative to comparison Group, in process group, mercury content reduces at least 40%, and cadmium content reduces at least 90%, and lead content reduces at least 25%.

(3) soil conditioner prepared by the solid content using the compositions of the application or composite bacteria agent capable to obtain after processing mud After, improving soil conditions to effect, add the nutritional labeling of soil, concrete data see embodiment.Wherein, soil porosity Being at least 40%, aeration porosity is at least 15%, and the content of organic matter is at least 1.0%.

Following example are merely to illustrate and the purpose of unrestricted the application scope.

Embodiment

Embodiment 1

Place: Weifang City of Shandong Province

Time: on March 15th, 2015

Temperature: 10 DEG C～20 DEG C

By bacillus subtilis solid fungicide, bacillus megaterium solid fungicide, Pseudomonas aeruginosa solid fungicide and addicted to Temperature Sphingobacterium solid fungicide mixes according to weight ratio 1:1:1:1, obtains composite bacteria agent capable A；Bacillus firmus is solid Body microbial inoculum, candida tropicalis solid fungicide and Lactobacillus plantarum solid fungicide mix than 1:1:1 according to dry weight, are answered Close microbial inoculum B.

This processes 800 cubic metres of Weifang City's desilting mud, average mark quinquepartite.Matched group adds pending mud body The powder of straw of long-pending 10% minces (corn straw, particle diameter is 5～15mm), is directly deposited in open area and processes, obtain after mixing The solid content of matched group.Process group first composite bacteria agent capable A and powder of straw are minced (corn straw, particle diameter is 5～15mm) mixing Uniformly, during then entirety joins pending mud, the water content of mixed material is 70%, carries out first fermentation.Wherein, process Group is by 0.5%, 1%, 2%, 3% addition composite bacteria agent capable A of pending mud weight, respectively process group 1, process group 2, process Group 3 and process group 4, each process group all add the powder of straw of pending sludge volume 10% mince (corn straw, particle diameter be 5～ 15mm).Carry out turning during first fermentation when temperature of charge is increased to 45 DEG C, within the most every 12 hours, carry out turning once, for the first time After fermenting 10 days, stop fermentation, respectively obtain process group 1 first fermentation product, process group 2 first fermentation product, process group 3 First fermentation product and process group 4 first fermentation product.Then according to 0.5% inoculation of process group 1 first fermentation products weight Composite bacteria agent capable B carries out ferment in second time, carries out secondary according to 1% inoculating compound bacterium agent B of process group 2 first fermentation products weight and sends out Ferment, carries out ferment in second time according to 2% inoculating compound bacterium agent B of process group 3 first fermentation products weight, first according to process group 4 3% inoculating compound bacterium agent B of tunning weight carries out ferment in second time, composite bacteria agent capable B is sprinkling upon each process group first fermentation and produces Thing surface, then uniform by mechanical mixture, ferment in second time continues 20 days every turning in 2 days, ferment in second time, respectively obtains process Solid content, the solid content of process group 2, the solid content of process group 3 and the solid content of process group 4 of group 1.Employing composite bacteria agent capable is harmless After change processes, the solid content of each process group and every physical and chemical index of the solid content of matched group and content of beary metal such as table 1 institute Show:

Every physical and chemical index of the solid content of table 1 each process group and matched group and content of beary metal

After Weifang City's riverway sludge is carried out microorganism harmless treatment, the water content of each process group riverway sludge is notable Reducing, rivers and lakes heavy metal in sludge content significantly reduces, and has reached rivers and lakes silt harmless.Along with composite bacteria agent capable connects Planting the increase of amount, treatment effect gradually strengthens.Relative to matched group, the moisture content of process group 1 reduces by 27.30%, and mercury content drops Low by 88.37%, arsenic content reduces by 66.42%, and cadmium content reduces by 97.29%, and chromium content reduces by 68.94%, and lead content reduces 88.17%；Relative to matched group, the moisture content of process group 2 reduces by 27.60%, and mercury content reduces by 90.70%, and arsenic content reduces 68.66%, cadmium content reduces by 98.64%, and chromium content reduces by 71.97%, and lead content reduces by 88.64%；Relative to matched group, place The moisture content of reason group 3 reduces by 33.80%, and mercury content reduces by 91.86%, and arsenic content reduces by 69.40%, and cadmium content reduces 98.64%, chromium content reduces by 72.73%, and lead content reduces by 88.49%；Relative to matched group, the moisture content of process group 4 reduces 37.00%, mercury content reduces by 94.19%, and arsenic content reduces by 72.39%, and cadmium content reduces by 99.32%, and chromium content reduces 75.76%, lead content reduces by 89.75%.

Embodiment 2

Place: Hongqiao town, Yueqing City, Wenzhou City, Zhejiang Province

Time: on June 10th, 2015

Temperature: 20 DEG C～30 DEG C

This processes 500 cubic metres of Wenzhou City's channel cleanout mud, is equally divided into two parts.Matched group adds pending silt The wood flour (particle diameter is 5～15mm) of mud volume 10%, is directly deposited in open area and processes, obtain the solid of matched group after mixing Thing.Process group is by composite bacteria agent capable A and wood flour (particle diameter is 5～15mm) mix homogeneously, during then entirety joins pending mud, Obtaining mixed material by mechanical mixture, the water content of mixed material is 70%, carries out first fermentation.Wherein, process group adds The composite bacteria agent capable A of the 2% of pending mud weight and the wood flour (particle diameter is 5～15mm) of pending sludge volume 10%.For the first time Carry out turning during fermentation when temperature of charge is increased to 45 DEG C, within the most every 12 hours, carry out turning once, first fermentation 10 days it After, stop fermentation, obtain first fermentation product.Then according to 2% inoculating compound bacterium agent B of first fermentation products weight carries out two Secondary fermentation, is sprinkling upon first fermentation its surface by composite bacteria agent capable B, then by mechanical mixture, ferment in second time every turning in 3 days, Ferment in second time continues 20 days, obtains the solid content of process group.After using composite bacteria agent capable harmless treatment, the solid content of process group As shown in table 2 with every physical and chemical index of the solid content of matched group and content of beary metal:

Every physical and chemical index of the solid content of table 2 each process group and matched group and content of beary metal

Note: "-" represents and do not detects.

After Wenzhou riverway sludge is carried out microorganism harmless treatment, the water content of each process group riverway sludge significantly drops Low, pH is basically unchanged, and after process, rivers and lakes heavy metal in sludge content significantly reduces, and has reached rivers and lakes mud harmless Change.Relative to matched group, process group moisture content reduces by 35.1%, and mercury content reduces by 93.59%, and lead content reduces by 87.69%, copper Content reduces by 53.99%, and Zn content reduces by 49.55%, and chromium content reduces by 72.46%, and arsenic content reduces by 93.06%, process group It is not detected by cadmium.

Embodiment 3

Place: Xi'an City, Shanxi Province

Time: on May 15th, 2015

Temperature: 20～30 DEG C

First mince mix homogeneously by composite bacteria agent capable A and powder of straw, during then entirety joins pending mud, passes through machine Tool is mixed to get mixed material, and the water content of mixed material is 70%, carries out first fermentation.Wherein, by pending mud weight 2% access composite bacteria agent capable A, and add pending sludge volume 10% powder of straw mince (corn straw, particle diameter be 5～ 15mm).Carry out turning during first fermentation when temperature of charge is increased to 45 DEG C, within the most every 12 hours, carry out turning once, for the first time After fermenting ten days, stop fermentation, obtain first fermentation product.Then according to 2% inoculation of first fermentation products weight is compound Microbial inoculum B carries out ferment in second time, and composite bacteria agent capable B is sprinkling upon first fermentation its surface, and then by mechanical mixture, ferment in second time is every Every turning in 3 days, ferment in second time continued 20 days, obtains solid content.

Before and after process, every physical and chemical index and the content of beary metal of rivers and lakes mud are the most as shown in table 3, relative to place Before reason, after process, moisture content reduces by 44.40%, and mercury content reduces by 69.74%, and arsenic content reduces by 71.13%, and cadmium content reduces 94.84%, chromium content reduces by 58.79%, and lead content reduces by 80.15%.

Every physical and chemical index of table 3 riverway sludge and content of beary metal

By solid content dry in the sun so that it is water content is reduced to less than 20%, dry solid content is pulverized, to pulverize Mud sieves, the granule of the particle diameter≤2.5mm obtained, and is soil conditioner, weighs the soil conditioner that said method prepares 10kg admixes 1m³In planting soil.

Aeration porosity=(the 1-soil weight/soil bulk density-soil weight × water capacity) × 100% formula I；

Soil improvement result is as shown in table 4 below:

Table 4 soil improvement index of correlation result

Soil conditioner prepared by the solid content that the composite bacteria agent capable using the application obtains after processing mud is improved effectively Soil conditions, adds the nutritional labeling of soil.

Embodiment 4

Place: Weifang City of Shandong Province

Time: on March 15th, 2015

Temperature: 10 DEG C～20 DEG C

By bacillus subtilis solid fungicide, bacillus megaterium solid fungicide and Pseudomonas aeruginosa solid fungicide according to Weight ratio 1:1:1 mixes, and obtains composite bacteria agent capable A1.

Bacillus subtilis solid fungicide, bacillus megaterium solid fungicide and mesophilic Sphingobacterium solid fungicide are pressed Mix according to weight ratio 1:1:1, obtain composite bacteria agent capable A2.

Bacillus subtilis solid fungicide, Pseudomonas aeruginosa solid fungicide and mesophilic Sphingobacterium solid fungicide are pressed Mix according to weight ratio 1:1:1, obtain composite bacteria agent capable A3.

Bacillus megaterium solid fungicide, Pseudomonas aeruginosa solid fungicide and mesophilic Sphingobacterium solid fungicide are pressed Mix according to weight ratio 1:1:1, obtain composite bacteria agent capable A4.

By bacillus firmus solid fungicide, candida tropicalis solid fungicide and Lactobacillus plantarum solid fungicide according to dry Anharmonic ratio 1:1:1 mixes, and obtains composite bacteria agent capable B.

By pending 1000 cubic metres of mud average mark quinquepartites.Matched group adds the straw of pending sludge volume 10% Stalk ground product (corn straw, particle diameter is 5～15mm), is directly deposited in open area and processes, obtain consolidating of matched group after mixing Shape thing.First mince with powder of straw respectively (Semen Maydis by composite bacteria agent capable A1, composite bacteria agent capable A2, composite bacteria agent capable A3 and composite bacteria agent capable A4 Straw, particle diameter is 5～15mm) mix homogeneously, during entirety joins each group of pending mud the most respectively, obtained by mechanical mixture To mixed material, the water content of mixed material is 70%, carries out first fermentation.Wherein, process group is respectively by pending mud weight 2% addition composite bacteria agent capable A1, composite bacteria agent capable A2, composite bacteria agent capable A3 and composite bacteria agent capable A4, respectively process group 1, the process group of amount 2, process group 3 and process group 4, each process group all adds the powder of straw of pending sludge volume 10% and minces (corn straw, particle diameter It is 5～15mm).Carry out turning during first fermentation when temperature of charge is increased to 45 DEG C, within the most every 12 hours, carry out turning once, After first fermentation ten days, stop fermentation, respectively obtain the first fermentation product of the first fermentation product of process group 1, process group 2 Thing, the first fermentation product of process group 3 and the first fermentation product of process group 4.Then according to the first fermentation of each process group produces The 2% of thing weight inoculating compound bacterium agent B respectively carries out ferment in second time, composite bacteria agent capable B is sprinkling upon first fermentation its surface, then By mechanical mixture, ferment in second time continues 20 days every turning in 3 days, ferment in second time, respectively obtains the solid content of process group 1, place The solid content of reason group 2, the solid content of process group 3 and the solid content of process group 4.After using composite bacteria agent capable harmless treatment, respectively The solid content of process group and every physical and chemical index of the solid content of matched group and content of beary metal are as shown in table 5:

Every physical and chemical index of table 5 each process group solid content and content of beary metal

After Weifang City's riverway sludge is carried out microorganism harmless treatment, in each process group, the water content of riverway sludge shows Writing and reduce, pH is basically unchanged, and after process, rivers and lakes heavy metal in sludge content significantly reduces, and has reached rivers and lakes mud Innoxious.Relative to matched group, the moisture content of process group 1 reduces by 25.20%, and mercury content reduces by 68.60%, and arsenic content reduces 52.99%, cadmium content reduces by 95.26%, and chromium content fall 50.00%, lead content reduces by 46.06%；Relative to matched group, process The moisture content of group 2 reduces by 23.70%, and mercury content reduces by 48.84%, and arsenic content reduces by 53.73%, and cadmium content reduces by 94.58%, Chromium content reduces by 62.88%, and lead content reduces by 67.67%；Relative to matched group, the moisture content of process group 3 reduces by 27.60%, Mercury content reduces by 59.30%, and arsenic content reduces by 47.01%, and cadmium content reduces by 97.29%, and chromium content reduces by 57.58%, and lead contains Amount reduction by 50.63%；Relative to matched group, the moisture content of process group 4 reduces by 30.00%, and mercury content reduces by 44.19%, and arsenic contains Amount reduction by 35.07%, cadmium content reduces by 95.26%, and chromium content reduces by 75.76%, and lead content reduces by 26.66%.

Although, the present invention is described in detail the most with a general description of the specific embodiments, but On the basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Cause This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.

Claims

1. a compositions, comprises bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus Megaterium), pseudomonas (Pseudomonadaceae), mesophilic Sphingobacterium (Sphingobacterium Thalpophilum), candida tropicalis (Candida tropicalis), bacillus firmus (Bacillus firmus) With in Lactobacillus plantarum (Lactobacillus plantarum) at least six kinds, such as, bacillus subtilis, huge spore bar Bacterium, pseudomonas, candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, Mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, addicted to Temperature Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, or, bacillus megaterium, pseudomonas, Mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum；Preferably comprise bacillus subtilis, Bacillus megaterium, pseudomonas, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum；More Preferably comprising compositions A and compositions B, described compositions A comprises bacillus subtilis, bacillus megaterium, pseudomonas With mesophilic Sphingobacterium, bacillus subtilis, bacillus megaterium and pseudomonas, bacillus subtilis, huge spore bar Bacterium and mesophilic Sphingobacterium, bacillus subtilis, pseudomonas and mesophilic Sphingobacterium, or bacillus megaterium, false list Born of the same parents bacterium and mesophilic Sphingobacterium；Described compositions B comprises candida tropicalis, bacillus firmus and Lactobacillus plantarum；

Optionally, described pseudomonas is Pseudomonas aeruginosa (Pseudomonas aeruginosa) or pseudomonas fluorescens (Pseudomonas fluorescens)。

2. a composite bacteria agent capable, its composition comprises bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), pseudomonas (Pseudomonadaceae), mesophilic Sphingobacterium (Sphingobacterium thalpophilum), candida tropicalis (Candida tropicalis), bacillus firmus In (Bacillus firmus) and Lactobacillus plantarum (Lactobacillus plantarum) at least six kinds, such as, hay bud Spore bacillus, bacillus megaterium, pseudomonas, candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis Bacterium, bacillus megaterium, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, hay spore Bacillus, pseudomonas, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, or, huge bud Spore bacillus, pseudomonas, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum；Preferably wrap Containing bacillus subtilis, bacillus megaterium, pseudomonas, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus Form with Lactobacillus plantarum or by above-mentioned seven kinds of bacterial strains；Or comprise the culture of above-mentioned at least six kinds of bacterial strains or seven kinds of bacterial strains Culture；More preferably comprise composite bacteria agent capable A and composite bacteria agent capable B, described composite bacteria agent capable A and comprise bacillus subtilis, huge bud Spore bacillus, pseudomonas and mesophilic Sphingobacterium, bacillus subtilis, bacillus megaterium and pseudomonas, hay spore Bacillus, bacillus megaterium and mesophilic Sphingobacterium, bacillus subtilis, pseudomonas and mesophilic Sphingobacterium, or huge Bacterium anthracoides, pseudomonas and mesophilic Sphingobacterium, or by bacillus subtilis, bacillus megaterium, pseudomonas Form with mesophilic Sphingobacterium these four bacterial strain, or comprise above-mentioned bacillus subtilis, bacillus megaterium, pseudomonas With the culture of at least three kinds of bacterial strains in mesophilic Sphingobacterium or the culture of described four kinds of bacterial strains；Described composite bacteria agent capable B bag Containing candida tropicalis, bacillus firmus and Lactobacillus plantarum, or it is made up of above-mentioned three kinds of bacterial strains, or comprises above-mentioned three Plant the culture of bacterial strain；

3. composite bacteria agent capable as claimed in claim 2, total living bacteria count >=1 × 10 in wherein said composite bacteria agent capable⁸cfu/g；

Total effectively viable bacteria it is highly preferred that described composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B, in described composite bacteria agent capable A Number >=1 × 10⁸cfu/g；Total living bacteria count >=1 × 10 in described composite bacteria agent capable B⁸cfu/g；

Or, in terms of the dry weight of described strain culture, bacillus subtilis culture, huge bud in wherein said composite bacteria agent capable Spore alphacterium culture, pseudomonas culture, mesophilic Sphingobacterium culture, candida tropicalis culture, strong spore In alphacterium culture and Lactobacillus plantarum culture, the weight portion of at least six kinds or seven kinds is:

Bacillus subtilis culture 2～5

Bacillus megaterium culture 1～3

Pseudomonas culture 2～4

Mesophilic Sphingobacterium culture 2～5

Candida tropicalis culture 3～5

Bacillus firmus culture 1～3

Lactobacillus plantarum culture 1～3,

Preferably, above-mentioned bacillus subtilis culture, bacillus megaterium culture, pseudomonas culture, the false silk in the torrid zone Dry weight between yeast culture, bacillus firmus culture and Lactobacillus plantarum culture is than for 1:1:1:1:1:1；

Preferably, above-mentioned bacillus subtilis culture, bacillus megaterium culture, mesophilic Sphingobacterium culture, heat With the dry weight between candida mycoderma culture, bacillus firmus culture and Lactobacillus plantarum culture than for 1:1:1:1:1: 1；

Preferably, above-mentioned bacillus subtilis culture, pseudomonas culture, mesophilic Sphingobacterium culture, torrid zone vacation Silk yeast culture, dry weight between bacillus firmus culture and Lactobacillus plantarum culture ratio is for 1:1:1:1:1:1；

Preferably, above-mentioned bacillus megaterium culture, pseudomonas culture, mesophilic Sphingobacterium culture, torrid zone vacation Silk yeast culture, dry weight between bacillus firmus culture and Lactobacillus plantarum culture ratio is for 1:1:1:1:1:1；

Preferably, the dry weight between above-mentioned seven kinds of strain cultures is than for 1:1:1:1:1:1:1；

It is highly preferred that described composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B, bacillus subtilis in described composite bacteria agent capable A Dry weight ratio between culture, bacillus megaterium culture, pseudomonas culture and mesophilic Sphingobacterium culture is 1:1:1:1；Bacillus subtilis culture, bacillus megaterium culture and pseudomonas culture in described composite bacteria agent capable A Between dry weight than for 1:1:1；Bacillus subtilis culture in described composite bacteria agent capable A, bacillus megaterium culture and addicted to Dry weight ratio between temperature Sphingobacterium culture is for 1:1:1；Bacillus subtilis culture, false list in described composite bacteria agent capable A Dry weight between born of the same parents' bacterium culture and mesophilic Sphingobacterium culture is than for 1:1:1；Or huge spore in described composite bacteria agent capable A Dry weight between alphacterium culture, pseudomonas culture and mesophilic Sphingobacterium culture is than for 1:1:1；

In described composite bacteria agent capable B candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture it Between dry weight than for 1:1:1；

Optionally, described culture comprises bacterial strain and carrier；

Optionally, described in described bacillus subtilis culture, the living bacteria count of bacillus subtilis is >=1 × 10⁸cfu/ g；Described in described bacillus megaterium culture, the living bacteria count of bacillus megaterium is >=1 × 10⁸cfu/g；Described vacation The living bacteria count of pseudomonas described in Pseudomonas culture is >=1 × 10⁸cfu/g；Described mesophilic Sphingobacterium is cultivated The living bacteria count of mesophilic Sphingobacterium described in thing is >=1 × 10⁸cfu/g；Institute in described candida tropicalis culture The living bacteria count stating candida tropicalis is >=1 × 10⁷cfu/g；Strong bud described in described bacillus firmus culture The living bacteria count of spore bacillus is >=1 × 10⁸cfu/g；Described in described Lactobacillus plantarum culture, Lactobacillus plantarum is effective Viable count is >=1 × 10⁸cfu/g；

4. a preparation method for composite bacteria agent capable, including:

By bacillus subtilis (Bacillus subtilis), bacillus megaterium (Bacillus megaterium), false list Born of the same parents bacterium (Pseudomonadaceae), mesophilic Sphingobacterium (Sphingobacterium thalpophilum), the false silk in the torrid zone Yeast (Candida tropicalis), bacillus firmus (Bacillus firmus) and Lactobacillus plantarum In (Lactobacillus plantarum) at least six kinds, such as, bacillus subtilis, bacillus megaterium, pseudomonas, Candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, bacillus megaterium, mesophilic sphingol bar Bacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, bacillus subtilis, pseudomonas, mesophilic sphingol bar Bacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum, or, bacillus megaterium, pseudomonas, mesophilic sphingol Bacillus, candida tropicalis, bacillus firmus and Lactobacillus plantarum；Preferably comprise bacillus subtilis, huge spore bar Bacterium, pseudomonas, mesophilic Sphingobacterium, candida tropicalis, bacillus firmus and Lactobacillus plantarum are cultivated respectively； And obtain the respective culture of described bacterial strain, and described culture is mixed according to combinations thereof, obtain described multiple Close microbial inoculum；

It is highly preferred that bacillus subtilis, bacillus megaterium, pseudomonas and mesophilic Sphingobacterium are trained respectively Support, it is thus achieved that the respective culture of described bacterial strain, and by described culture according to described bacillus subtilis, huge spore bar Bacterium, pseudomonas and mesophilic Sphingobacterium, bacillus subtilis, bacillus megaterium and pseudomonas, bacillus subtilis, Bacillus megaterium and mesophilic Sphingobacterium, bacillus subtilis, pseudomonas and mesophilic Sphingobacterium, or, huge bud The combination of spore bacillus, pseudomonas and mesophilic Sphingobacterium carries out being mixed to get described composite bacteria agent capable A；By false for torrid zone silk ferment Mother, bacillus firmus and Lactobacillus plantarum are cultivated respectively, it is thus achieved that the respective culture of described bacterial strain, and by described training Support thing to carry out being mixed to get described composite bacteria agent capable B according to combinations thereof；Described composite bacteria agent capable includes composite bacteria agent capable A and composite bacteria agent capable B；

5. method as claimed in claim 4, wherein mixes in terms of the dry weight of the respective culture of described bacterial strain, mixing Time bacillus subtilis culture, bacillus megaterium culture, pseudomonas culture, mesophilic Sphingobacterium culture, At least six kinds or the weight of seven kinds in candida tropicalis culture, bacillus firmus culture and Lactobacillus plantarum culture Part is:

Bacillus subtilis culture 2～5

Bacillus megaterium culture 1～3

Pseudomonas culture 2～4

Mesophilic Sphingobacterium culture 2～5

Candida tropicalis culture 3～5

Bacillus firmus culture 1～3

Lactobacillus plantarum culture 1～3,

It is highly preferred that described composite bacteria agent capable comprises composite bacteria agent capable A and composite bacteria agent capable B, bacillus subtilis in described composite bacteria agent capable A Dry weight ratio between culture, bacillus megaterium culture, pseudomonas culture and mesophilic Sphingobacterium culture is 1:1:1:1；Bacillus subtilis culture, bacillus megaterium culture and pseudomonas culture in described composite bacteria agent capable A Between dry weight than for 1:1:1；Bacillus subtilis culture in described composite bacteria agent capable A, bacillus megaterium culture and addicted to Dry weight ratio between temperature Sphingobacterium culture is for 1:1:1；Bacillus subtilis culture, false list in described composite bacteria agent capable A Dry weight between born of the same parents' bacterium culture and mesophilic Sphingobacterium culture is than for 1:1:1；Or, huge bud in described composite bacteria agent capable A Dry weight between spore alphacterium culture, pseudomonas culture and mesophilic Sphingobacterium culture is than for 1:1:1；

Optionally, described culture comprises bacterial strain and carrier；

6. process or a method for aid in treatment mud, including:

By compositions described in claim 1, or composite bacteria agent capable described in Claims 2 or 3 is inoculated in pending mud and locates Manage at least 5 days, at least 10 days, at least 15 days, at least 20 days, at least 25 days, at least 30 days, at least 35 days, at least 40 days or extremely Few 45 days, preferably 30 days；

Preferably, the mud during described pending mud refers to river course, lake or blowdown canal；

Preferably, described compositions or described composite bacteria agent capable relative to the inoculum concentration of described pending mud be at least 0.1%, extremely Few 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w；

Optionally, described pending mud can add adjuvant, such as: wood flour or straw ground product.

7. method as claimed in claim 6, the method comprise the steps that

By compositions A described in claim 1, or carry out during described in Claims 2 or 3, composite bacteria agent capable A is inoculated into pending mud Process at least 5 days, at least 10 days or at least 15 days, preferably at least 10 days, obtain first fermentation material；Then by claim Compositions B described in 1, or carry out during described in Claims 2 or 3, composite bacteria agent capable B is inoculated into first fermentation material processing at least 5 days, At least 10 days, at least 15 days, at least 20 days, at least 25 days or at least 30 days, preferably at least 20 days；

Preferably, described compositions A or described composite bacteria agent capable A relative to pending mud inoculum concentration be at least 0.1%, extremely Few 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w, more preferably at least 0.5%, At least 1%, at least 2% or at least 3%w/w；Described compositions B or described composite bacteria agent capable B connecing relative to first fermentation material The amount of kind is at least 0.1%, at least 0.5%, at least 1%, at least 1.5%, at least 2%, at least 2.5% or at least 3%w/w, more It is preferably at least 0.5%, at least 1%, at least 2% or at least 3%w/w.

8. the solid content that method described in claim 6 or 7 obtains after processing described pending mud.

9. a soil conditioner, the solid content obtained after comprising the described pending mud of process described in claim 8；

Preferably, the water content of described soil conditioner is≤30% ,≤25% ,≤20% ,≤15% ,≤10% or≤5%, More preferably≤20%；

Preferably, the particle diameter of described soil conditioner is≤1mm ,≤2.5mm ,≤3mm ,≤5mm ,≤7.5mm or≤10mm, more It is preferably≤2.5mm.

10. a method for soil improvement, including:

By soil improvement described in the solid content obtained after the described pending mud of process described in claim 8 or claim 9 Agent joins and carries out processing at least 5 days, at least 10 days, at least 15 days, at least 20 days, at least 25 days or at least in soil to be improved 30 days, preferably at least 20 days；

Preferably, the solid content obtained after the described described pending mud of process or described soil conditioner are relative to waiting to improve The addition of soil is at least 5, at least 10, at least 15 or at least 20kg/m³, more preferably at least 10kg/m³。