CN105964680B - A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof - Google Patents

A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof Download PDF

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CN105964680B
CN105964680B CN201610301552.5A CN201610301552A CN105964680B CN 105964680 B CN105964680 B CN 105964680B CN 201610301552 A CN201610301552 A CN 201610301552A CN 105964680 B CN105964680 B CN 105964680B
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soil
cotton
agent
culture
saline land
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CN105964680A (en
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陈建爱
陈为京
杨武汉
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Institute of Agro Food Science and Technology of Shandong Academy of Agricultural Sciences
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Institute of Agro Food Science and Technology of Shandong Academy of Agricultural Sciences
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00—Methods for working soil
    • A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
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  • Environmental Sciences (AREA)
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  • Mycology (AREA)
  • Environmental & Geological Engineering (AREA)
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Abstract

The present invention relates to a kind of beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent, including following component, are parts by weight: 2-200 parts of trichoderma microbial bacterial agent, 5-300 parts of potassium fulvate, other organic matters add 10-900 parts of fertilizer.It is repairing the application in beach saline land Cotton Soil ecology.It can promote the formation of soil w ater-stable aggregates, improve soil texture, promote the foundation and holding of soil beneficial microbe group, protect root system of plant, contend with germ, improve disease resistance of plant；Science combines new food chain, overall balance plant demand, utilize its stronger complexing power, improve the absorption and operating of micro elements by plants, promote absorption of the crop to nitrogen, phosphorus, potassium and microelement, improve utilization rate of fertilizer, effectively overcome weighed with the poor, dystrophia that solves that there are soil fertility in beach saline land cotton field, the soil-borne disease unfavorable factors such as again, provide technical support for the sustainable efficient utilization in existing cotton field.

Description

A kind of beach saline land continuous cropping cotton soil ecology renovation agent and preparation method thereof with Using
Technical field
The invention belongs to soil ecology renovation agent technical fields, and in particular to a kind of beach saline land continuous cropping cotton soil is raw State renovation agent and the preparation method and application thereof.
Background technique
China's saline alkali land resource very abundant all has scale not equal saline alkali land resource in main cotton growing area, all in all, The potentiality for developing alkaline land cotton production are very big.Shandong is one of the most abundant province of national saline alkali land resource, beach saline land It is mainly distributed on Bohai Sea Gulf south bank, the Huanghe delta and Laizhou Bay Coastline, the gross area about 800khm2, after having opened up wasteland or having improved Plant the salt-soda soil cotton field about 300khm of cotton2.Due to inwelling, soil transpiration etc., cause beach saline land cotton field saliferous Amount continues higher, and cotton grower only plants salt tolerant crop cotton year after year, leads to beach saline land cotton field there are soil fertility poor, nutrition Obstacle weight, soil-borne disease many unfavorable factors such as again, seriously restrict the raising of coastal saline soil cotton production level.Therefore, On the one hand it develops and uses the Qu Yimian saline-alkali wasteland resource and expands plant cotton area, on the other hand improved by soil ecology reparation existing There are the yield and benefit of beach saline land continuous cropping cotton field cotton, Significance of Sustainable Development weight is produced for Shandong or even national cotton Greatly.
About new saline-alkali wasteland resource is developed and used, domestic and international researcher is in system research soil, water body, plant Warping pressure alkali is proposed on the basis of, weather, is ploughed deeply nest salt, pressure sand alkali resistant, green manure and is turned over polymer chemistry modifying agent etc. Method, but these methods are mainly for the salination of coastal saline-alkali soil and the soil swelling-shrinkage of meta-alkali, to existing coastal saline-alkali Many unfavorable factors overcome and solve again etc. for there are soil fertility in ground continuous cropping cotton cotton field poor, dystrophia weight, soil-borne disease Effect is not significant.
104152149 A of CN disclose it is a kind of containing rhizopus stolonifer fermentation liquid 20-40, Trichoderma Viride liquid 20-40, The novel and multifunctional compound biological soil improver of water-soluble chitosan 1.5-2.5, potassium fulvate 1.5-4.5.This application is soil The secondary reclamation of salinep-alkali soil in earth surface layer is to improve soil pH value, improvement acidified soil, hardened soil and secondary salinization soil； Activating soil nutrient discharges soil fertility, is not particularly suited for new saline-alkali wasteland improvement.
CN101717646A discloses one kind and contains sheep dung, potassium fulvate, Kang Dibao, manganese sulfate, zinc sulfate, trichoderma, fly The soil conditioner of the prevention and treatment take-all of maggot powder, leftover bits and pieces.Primarily directed to newlyexploited land soil depletion and wheat total eclipse Serious problem occurs for disease, reaches and adjusts soil texture, increase soil fertility, enhance crop disease-resistant ability, reduce wheat total eclipse Pathogenetic effect can not also provide technical inspiration with not being a thinking to alkaline land improving.
Summary of the invention
That the present invention is directed to the above beach saline land continuous cropping cotton existing in the prior art cotton field there are soil fertility is poor, seeks Support obstacle weight, soil-borne disease again etc. many unfavorable factors the problem of, providing one kind, to can obviously reduce coast saline soil water-soluble Property salt content, reduce soil pH value, induction crop to the soil salinization generate stress tolerance, promote root system development and the production of crop Measure the beach saline land continuous cropping cotton soil ecology renovation agent formed.
The present invention also provides the preparation methods of the beach saline land continuous cropping cotton soil ecology renovation agent.
The present invention also provides the applications of the beach saline land continuous cropping cotton soil ecology renovation agent.
The present invention is achieved by the following measures:
A kind of beach saline land continuous cropping cotton soil ecology renovation agent, including following component, are parts by weight:
2-200 parts of trichoderma microbial bacterial agent, 5-300 parts of potassium fulvate, other organic matters add 10-900 parts of fertilizer；
Effective viable bacteria of the trichoderma microbial bacterial agent is trichoderma (Trichoderma sp.) T-15580, culture presevation Number: CGMCC No.12162, spore content 2-50 × 108cfu/g；
The potassium fulvate is a kind of product being commercialized, and certain fulvic acid and oxidation are extracted from humic acid Potassium is made, and appearance is chocolate brown powder.Main component: fulvic acid content (in terms of butt) % >=50%, full nitrogen (N) content (with Butt meter) % >=3.0%, full phosphorus (P) content (in terms of butt) % >=0.4%, full potassium (K02) content (in terms of butt) % >= 11.7%, PH 5.7.
The beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent, preferred raw material weight proportion are as follows:
Spore content 6-36 × 108(preferred 6-12 × 10 cfu/g8cfu/g、12-36×108Cfu/g trichoderma) is micro- 20-100 parts of bacteria agent, 20-50 parts preferred, 50-100 parts, 80-300 parts of potassium fulvate, 80-150 parts preferred, 150-300 parts, 700-900 parts of organic fertilizer auxiliary material, 700-800 parts preferred, 800-900 parts.
A kind of beach saline land continuous cropping cotton soil ecology renovation agent and preparation method thereof, steps are as follows:
(1) take strain trichoderma T-15580, through actication of culture, seed culture, expand culture obtain spore content 2-50 × 108The trichoderma microbial bacterial agent of cfu/g；
(2) to get beach saline land Cotton Soil after trichoderma microbial bacterial agent and potassium fulvate, organic fertilizer auxiliary material being mixed Restoration of the ecosystem agent.
Actication of culture condition in the step (1) are as follows: in 26 DEG C of -28 DEG C of culture 70-90h, culture medium is PDA culture Base；
Seed culture condition are as follows: shaken cultivation 36-48h under the conditions of 25 DEG C -27 DEG C, 100-180r/min, culture medium are PD culture medium；
Expand culture incubation step are as follows: under the conditions of 24 DEG C -26 DEG C, stir culture 36-48h, liquid in fluid nutrient medium Culture medium is trichoderma microbial liquid culture medium；It is forwarded in solid medium, then under the conditions of 22 DEG C -28 DEG C, solid culture 70-100h is cultivated in base, solid medium is trichoderma microbial solid culture medium.
Above-mentioned trichoderma microbial liquid nutrient media components are as follows: 45-55g corn flour, adds water to 1000ml at 5-8g bean cake powder, Sterilizing, filtering.
Above-mentioned trichoderma microbial solid nutrient media components are as follows: use wheat bran 60-80wt%, cotton seed hulls 20-40wt%；Or Person, wheat bran 40-50wt%, rice husk 10-25wt%, corn stover 20-30wt%, corncob 10-25wt%.
Other culture mediums are culture medium commonly used in the art, such as:
PDA culture medium: potato 200g, glucose 20g, agar powder 15g, water 1000mL；
PD culture medium: potato 200g, glucose 20g, water 1000mL；
Above-mentioned beach saline land continuous cropping cotton soil ecology renovation agent is in repairing beach saline land Cotton Soil ecology Using preferable amount is 60-250kg/ mus.
Beneficial effect
1, beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent of the present invention can be salinized soil continuous cropping obstacle soil Source of life is provided, science combines new food chain, overall balance plant demand.The restoration of the ecosystem agent of this soil removes the Huang of high-content Rotten acid is outer, also (oligomeric rich in almost all amino acid needed for growing process, nitrogen, phosphorus, potassium, a variety of enzymes, carbohydrate Sugar, fructose etc.), protein, nucleic acid, humic acid and the nutritions such as VC, VE and a large amount of B family vitamin, be a kind of green Bioremediation agents；
2, beach saline land continuous cropping cotton soil ecology renovation agent of the present invention can be newly to open up wasteland strong salinized soil soil Structural development provides builder, and trichoderma microorganism can promote the formation of soil w ater-stable aggregates, improves soil texture, promotes The foundation and holding of soil beneficial microbe group, improve biological activity of soil, trichoderma microorganism as analyst in the soil Macromolecular substances effect of degrading is obvious, and the plant and animal residues in soil can be degraded to small-molecule substance by secretion hydrolase, both Clean beach saline land Cotton Soil ecological environment, and abundant soil nutrient, moreover it is possible to which activated mineral improves soil, improves Soil productivity improves the quality of soil, provides technical support for efficient utilize in existing cotton field；
3, it is water-soluble to can obviously reduce strong coast saline soil for beach saline land Cotton Soil restoration of the ecosystem agent of the present invention Property salt content, reduce soil pH value, induction crop to the soil salinization generate stress tolerance, promote root system development and the production of crop Amount is formed；
4, the trichoderma microorganism of beach saline land Cotton Soil restoration of the ecosystem agent of the present invention promotes the micro- life of beneficial rhizosphere Object growth, protects root system of plant, contends with cotton verticillium wilt, Sclerotium rolfsii, and then inhibits the growth of crop soil-borne disease bacterium, improves Disease resistance of plant overcomes continuous cropping obstacle, promotes plant growth, improves crop yield and quality；
5, beach saline land continuous cropping cotton soil ecology renovation agent trichoderma and fulvic acid mutual reciprocity and mutual benefit of the present invention, it is yellow rotten Acid can become the nutriment of trichoderma growth under certain conditions, and the colonization ability for increasing trichoderma improves trichoderma proliferative amount, wood Mould metabolite provides plant hormone, hydrolase and biochemical activity substance for fulvic acid, further increases the activity of fulvic acid, increases Strong fulvic acid effect；
6, beach saline land continuous cropping cotton soil ecology renovation agent complexing power of the present invention is strong, improves minimum plant member The absorption and operating of element.One of this soil restoration of the ecosystem agent component potassium fulvate is due to total amino acid and carboxyl, hydroxyl isoreactivity Group content is higher, and has oxygen-containing functional group, and there are many organic chelating positions and complexing position, these coordinating group energy in structure With many microelements such as the calcium of indissoluble, magnesium, sulphur, iron, manganese, copper, zinc, boron occur complexing or it is chelating react, while also can Complex reaction occurs with phosphorus element on playing his site, to be formed using fulvic acid molecule as intermediary's carrier, while coordinating, promoting to plant Absorption, the operating of object root system or blade face to microelement and phosphorus in vivo, not only avoid direct contact of the microelement with phosphorus And cause to be passivated inactivation each other, and play positive balanced action, to improve the utilization rate of the two；
7, beach saline land Cotton Soil restoration of the ecosystem agent of the present invention can promote crop to nitrogen, phosphorus, potassium and micro member The absorption of element improves utilization rate of fertilizer, while having the function of buffering fertilizer efficiency, when soil and fertilizer nutrient is excessive, the renovation agent In microorganism absorb inorganic nutrients abundant and be housed in thallus, gradually decrease rhizosphere nutrient density, when soil nutrient not When sufficient, with the death of microorganism, gradually released again by the nutrient that thallus absorbs, for crop absorption utilization.
Culture presevation information
The preservation time: on 2 25th, 2016,
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Deposit number: CGMCC No.12162,
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, it is postal Coding: 100101 classification namings: trichoderma Trichoderma sp..
Specific embodiment
Below with reference to example, the technical solution of the present invention is further described in detail, but embodiments of the present invention are not limited to This.
Potassium fulvate described in embodiment is purchased from Shandong Creation Hhumic Acid Science and Technology Co., Ltd, and cotton LMY-36 is purchased from Shandong Cotton Research Center.
Acquisition, screening and the preservation of 1 bacterial strain of embodiment
Culture medium:
PDA culture medium: potato 200g, glucose 20g, agar 15g, water 1000mL；
Martin (Martin) culture medium: peptone 5g, glucose 10g, KH2PO41g, MgSO4.7H2O 0.5g, agar 20g, rose-bengal (1%) 3.3mL, 33 milligrams of streptomysin, pH 7.0-7.2, water 1000mL.
The acquisition of bacterial strain:
In slight alkaline land cotton yellow wilt disease morbidity field, 1- is removed around morbidity cotton and cotton rhizosphere of not falling ill 5cm topsoil takes topsoil soils to be packed into paper bag and takes back laboratory separation.A series of concentration are made in pedotheque, are taken certain dense Degree dilution is coated on Martin's culture medium flat plate, 26 DEG C of -28 DEG C of culture 3-4d, and picking form is transferred like the single colonie of Trichoderma Purifying culture is carried out on to PDA culture medium, is numbered after inspection is initially identified as trichoderma, and inclined-plane culture cryo-conservation.
Through the above steps, the bacterial strain for obtaining many pure cultures carries out the speed of growth, sporulation quantity etc. to serial trichoderma strain Shape compares, and primary dcreening operation obtains preferable 2 bacterial strains and cultivates a large amount of reesei spores of acquisition respectively, is made 107-108Cfu/mL spore is outstanding Supernatant liquid, is separately added into 0.01mol/L sodium nitrite solution mutagenesis 20min, then lethality takes respectively in 70%-80% 0.1mL is spread evenly across on PDA plate, is placed away from irradiating 3min under 20W ultraviolet lamp 20cm, and lethality is in 80%-90%, and 25 DEG C culture 12h-24h, monospore picking sprout single bacterium body in being cultivated on PDA plate, further choose normal growth mutant bacteria In strain squamous subculture 5-10 generation, after bacterial strain is stablized, serial mutagenic strain and former Strain comparison, primary dcreening operation obtain more preferably new strains.
Dominant strain is grown in post-directed training base, the culture medium of salt concentration 6 ‰ -10 ‰ obtains salt-enduring strain, with Cotton yellow Sclerotium rolfsii (verticillium dahliae Verticillium dahliae；Cotton wilting sickle-like bacteria Fusarium Vasinfectum be control with starting strain) in opposite culture in PDA culture medium, obtain Antagonism have than starting strain it is larger Improve new dissociant, will wherein one plant be named as T-15580.Its increment, sporulation quantity and to germ inhibiting effect, cover The indexs such as lid effect improve 4.5%, 10.2%, 41.1%, 11.4% than starting strain respectively.
The morphological feature of bacterial strain:
The growth of trichoderma (Trichoderma spp.) bacterium colony is fast, and aging culture colony colour is green, aromatic, sporophore Cong Shu is annularly arranged.Secondary branch is single or is stretched out with 2-3 for one group of wide-angle, and branch is usually bent or in wavy, bottle Metulae portion hangs contracting, and centre is expanded, straight or bending, under ultimate bottle stalk, 2-3 bottle stalk with the arrangement of false wheel mode, once in a while individually or It stretches out in pairs.Conidium is subsphaeroidal, and size is 3.4-4.3 × 2.6-3.4um.
2 soil-repairing agent of embodiment is to strong salinized soil salt reduction alkali and improves soil biomass effect
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each component parts by weight are as follows:
20 parts of trichoderma microbial bacterial agent, 80 parts of potassium fulvate, 900 parts of farmyard manure；
Above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent is prepared as follows:
(1) preparation of trichoderma microbial bacterial agent:
Actication of culture
T-15580 is the separation of oneself laboratory, and by the various growth limit conditional filterings acquisitions such as with high salt, and with it is current Bio-control factors act on strong T22 strain and inhibit pathogen growth comparative test.
Taking culture presevation number is the trichoderma T-15580 of CGMCC No.12162, is inoculated in 26 DEG C of cultures in PDA culture medium 72h obtains activation trichoderma T-15580；
PDA culture medium: potato 200g, glucose 20g, agar powder 15g, water 1000mL.
Seed culture
Trichoderma T-15580 spore sterile water will be activated and dilute the 250mL triangular flask kind that access is equipped with 50mLPD culture medium, 26 DEG C, 180r/min, shaken cultivation 72h, obtain seed trichoderma T-15580；
PD culture medium: potato 200g, glucose 20g, water 1000mL.
Expand culture
(i) 100mL seed trichoderma T-15580 is inoculated in 16 liters of fermentations equipped with the mould microbial liquid culture medium of 7 litres In tank, stir culture 40h at 26 DEG C obtains trichoderma T-15580 liquid；
Trichoderma microbial liquid culture medium: 47.5g corn flour, 7.5g bean cake powder add water to 1000mL, sterilizing, filtering.
(ii) trichoderma T-15580 liquid being moved into trichoderma microbial solid culture medium with the ratio of 5wt%, tray is placed, Space relative humidity 70%-90%, well-ventilated periodically turn over, 26 DEG C of culture 96h, obtain reesei spores content 36 × 108The trichoderma microbial bacterial agent of cfu/g.
Trichoderma microbial solid culture medium: after being mixed using 80wt% wheat bran with 20wt% cotton seed hulls, sterilizing.
(2) to get shore after trichoderma microbial bacterial agent, potassium fulvate, farmyard manure made from step (1) being mixed by weight Extra large salt-soda soil Cotton Soil restoration of the ecosystem agent.
Test example
Test plot: Dongying city is with having opened up wasteland Kenli County the strong salinized soil continuous cropping in strand
Because the plot is to have opened up wasteland plot, before spring ploughing, field vegetation dry weight is less than every 667 square metres of 100kg, by the ground Block is divided into two pieces of plot of soil-repairing agent group and control group:
Modifying agent group turns over irrigation by the application coast saline soil restoration of the ecosystem agent of 250kg/ mu amounts, real-time sowing cotton, Cultivation management is operated by conventional tillage management；
Control group does not apply coast saline soil restoration of the ecosystem agent, other are the same as renovation agent group.
Test data and experimental result:
1 soil-repairing agent of table is emerged to the strong salinized soil cotton in strand and the influence of yield
Experimental result shows that beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves the emergence rate of cotton 32.7%, planting percent improves 48.9%, solves the problems, such as that the strong difficult and vertical seedling of alkaline land cotton emergence is difficult, promotes cotton normal season Section is bloomed and blow-of-cottons, and cotton boll blow-of-cottons are concentrated, and increases substantially than flower before control frost, flower rate improves 409.7% before frost, cotton quality It improves, yield increases substantially.
Regulating effect of 2 soil-repairing agent of table to strand strong salinized soil continuous cropping cotton field microorganism
Regulating effect of 3 soil-repairing agent of table to strand strong salinized soil continuous cropping cotton field microbial population
Processing Bacterial species Actinomyces Fungal species The soil-borne diseases bacterium such as fusarium
Renovation agent group 4 kinds or more There is certain amount 3 kinds or more It is not separated to
Control group It is single Do not turn out It is single 3 kinds
The result shows that beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves strong salinized soil, soil is increased substantially Earth micro organism quantity, microbe species increase, and the beneficial microbes such as actinomyces increase, and optimize structure of soil microbial community, soil The harmful microorganisms such as fusarium type and quantity substantially reduce in earth.
4 soil ecology renovation agent of table improves situation to the strong salinized soil continuous cropping in strand cotton field physicochemical character
As the result is shown: physics and chemistry of the coast saline soil restoration of the ecosystem agent of the present invention to the strong salinized soil in Dongying City Kenli County strand Character has apparent improved effect.Soil organic matter content is improved, coast saline soil water soluble salt is significantly reduced and contains Amount, reduces soil pH value, significantly improves soil w ater-stable aggregates content.
3 soil-repairing agent of embodiment improves soil vitality and output of cotton effect to moderate salinized soil
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each component parts by weight are as follows:
50 parts of trichoderma microbial bacterial agent, 150 parts of potassium fulvate, 800 parts of livestock and poultry muck；
Above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent preparation method is as described in Example 1, the difference is that:
Trichoderma microbial bacterial agent is reesei spores content 12 × 108The trichoderma microorganism of cfu/g；
To get beach saline land cotton field soil after trichoderma microbial bacterial agent, potassium fulvate, livestock and poultry muck are mixed by weight Earth restoration of the ecosystem agent.
Test example
Test plot: Dongying city is with having opened up wasteland Kenli County strand moderate salinized soil continuous cropping
Because the plot is to have opened up wasteland plot, before spring ploughing, field vegetation dry weight is less than every 667 square metres of 100kg, by the ground Block is divided into two pieces of plot of soil-repairing agent group and control group:
Modifying agent group turns over irrigation by the application coast saline soil restoration of the ecosystem agent of 150kg/ mu amounts, real-time sowing cotton, Cultivation management is operated by conventional tillage management；
Control group does not apply coast saline soil restoration of the ecosystem agent, other are the same as renovation agent group.
Test data and experimental result:
Influence of 5 soil-repairing agent of table to strand moderate salinized soil Developmental of Cotton
Experimental result shows that beach salty soil continuous cropping cotton soil-repairing agent of the present invention promotes cotton growth, plant height It improves, the renovation agent lasts phase is long, adjusts Developmental of Cotton, and cotton blooms concentrations, and cotton boll blow-of-cottons are concentrated, and spends rate big before frost Amplitude improves, and cotton quality improves, and yield increases substantially.
Regulating effect of 6 soil-repairing agent of table to strand moderate salinized soil continuous cropping cotton field microbial population
Experimental result shows that beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves soil microbe quantity, puts The beneficial microbes such as line bacterium, nitrogen-fixing bacteria, photosynthetic bacteria increase, the decline of the soil-borne diseases bacterium number amount such as fusarium.
Regulating effect of 8 soil-repairing agent of table to strand moderate salinized soil continuous cropping Cotton Soil hydrolytic enzyme activities
Experimental result shows that beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves moderate salinized soil continuous cropping soil Loam Soil enzyme increases the biochemical reaction ability of soil, improves soil vitality, increases soil soluble nutrition, promotes Cotton growth and development improve Resistance Strain of Cotton adverse circumstance ability.
4 soil-repairing agent of embodiment prevents and treats cotton yellow wilt disease effect to slight salinized soil
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each component parts by weight are as follows:
100 parts of trichoderma microbial bacterial agent, 300 parts of potassium fulvate, 700 parts of organic fertilizer；
Above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent preparation method is as described in Example 1, the difference is that:
Trichoderma microbial bacterial agent is reesei spores content 6 × 108The trichoderma microorganism of cfu/g；
To get beach saline land Cotton Soil after trichoderma microbial bacterial agent, potassium fulvate, organic fertilizer are mixed by weight Restoration of the ecosystem agent.
Test example
Test plot: Dongying city Kenli County strand is slight salinized soil continuous cropping cotton
The plot is divided into two pieces of plot of renovation agent group and control group, renovation agent group presses 60kg/ mus of application beach salty soils Continuous cropping cotton soil ecology renovation agent, turns over irrigation, real-time sowing cotton, and cultivation management is operated by conventional tillage management；
Control group does not apply coast saline soil restoration of the ecosystem agent, other are the same as modifying agent group.
Test data and experimental result:
9 soil-repairing agent of table is to the slight salinized soil cotton yellow wilt disease disease-controlling effect in strand
9 result of table explanation, beach salty soil continuous cropping cotton soil-repairing agent of the present invention increase substantially the lateral root number of cotton, The growth for inhibiting soil-borne disease improves the disease resistance of cotton, improves output of cotton, improves the income of farmer.
Regulating effect of 10 soil-repairing agent of table to strand slight salinized soil continuous cropping cotton field microbial population
Table 10 the result shows that, beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves soil microbe quantity, changes Good soil microbial community composition, the beneficial microbes such as nitrogen-fixing bacteria, photosynthetic bacteria, cellulose-decomposing bacterium increase, reaping hook in soil The harmful microorganisms quantity such as bacterium substantially reduces.
11 soil ecology modifying agent of table improves situation to the slight salinized soil physicochemical character in strand
Table 11 is the testing result after beach salty soil continuous cropping cotton soil-repairing agent of the present invention application soil after 78 days, inspection Mark is quasi-: hydrolyzable nitrogen is according to People's Republic of China (PRC) agricultural industry criteria NY/T 1848-2010, available phosphorus according to the Chinese people Republic agricultural industry criteria NY/T 1121.7-2006, available potassium are according to People's Republic of China (PRC) forestry industry standard LY/T 1236-1999, water soluble salt are according to People's Republic of China (PRC) agricultural industry criteria NY/T 1121.16-2006, pH value in Magnificent people's republic's agricultural industry criteria NY/T 1121.2-2006, water-stable aggregate are according to People's Republic of China's agricultural row Industry standard NY/T 1121.19-2008.As a result illustrate, the application of soil-repairing agent, solable matter increases in soil, improves soil The utilization rate of earth nutrition is conducive to cotton root system and absorbs, utilizes, promotes cotton growth, while soil w ater-stable aggregates increase, and change Kind soil texture.
In summary experimental result, coast saline soil restoration of the ecosystem agent of the present invention significantly improve soil microbe quantity, Soil microbial population structure is improved, soil organic matter content is improved, improves soil w ater-stable aggregates quantity, is reduced yellow River delta coast saline soil water soluble salt content reduces soil pH value, improves biological activity of soil, higher in salinity Strong salinized soil significantly improve the emergence rate and planting percent of cotton, inhibit the growth of soil-borne disease, inducing cotton is to soil salt marsh Change and generate stress tolerance and disease resistance, promotes the root system development and yield composition of crop.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by the limit of embodiment System, other any changes made without departing from the spirit and principles of the present invention, modification, combination, substitution, simplification should be Equivalence replacement mode, is included within the scope of the present invention.

Claims (11)

1. a kind of beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent, it is characterised in that raw material weight proportioning of soup processed is as follows:
2-200 parts of trichoderma microbial bacterial agent, 5-300 parts of potassium fulvate, 10-900 parts of organic fertilizer auxiliary material；
Effective viable bacteria of the trichoderma microbial bacterial agent is trichoderma T-15580, culture presevation number: CGMCC No.12162, mitogenetic Spore content 2-50 × 108cfu/g。
2. beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent according to claim 1, it is characterised in that raw material weight It matches as follows:
Conidium content 6-36 × 10880-300 parts of 20-100 parts of trichoderma microbial bacterial agent, potassium fulvate, the organic fertilizer of cfu/g 700-900 parts of auxiliary material.
3. a kind of preparation method of beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent of any of claims 1 or 2, feature It is
(1) strain trichoderma T-15580 is taken, through actication of culture, seed culture, expands culture acquisition trichoderma microbial bacterial agent；
(2) after trichoderma microbial bacterial agent and potassium fulvate, organic fertilizer auxiliary material being mixed to get.
4. preparation method according to claim 3, it is characterised in that the actication of culture condition in step (1) are as follows: 26 DEG C- 28 DEG C of culture 70-90h, culture medium is PDA culture medium.
5. preparation method according to claim 3, it is characterised in that the seed culture condition in step (1) are as follows: 25 DEG C- 27 DEG C, shaken cultivation 36-48h under the conditions of 100-180r/min, culture medium are PD culture medium.
6. preparation method according to claim 3, it is characterised in that the expansion culture in step (1) are as follows: at 24 DEG C -26 DEG C Under the conditions of, stir culture 36-48h in fluid nutrient medium, fluid nutrient medium be trichoderma microbial liquid culture medium；It is forwarded to solid In culture medium, then 70-100h is cultivated under the conditions of 22 DEG C -28 DEG C, be trichoderma microbial solid culture medium in solid medium.
7. preparation method according to claim 6, it is characterised in that trichoderma microbial liquid nutrient media components described in step Are as follows: 45-55g corn flour, 5-8g bean cake powder add water to 1000mL, sterilizing, filtering.
8. preparation method according to claim 6, it is characterised in that the trichoderma microbial solid nutrient media components are as follows:
Wheat bran 60-80wt%, cotton seed hulls 20-40wt%；
Alternatively, wheat bran 40-50wt%, rice husk 10-25wt%, corn stover 20-30wt%, corncob 10-25wt%.
9. in a kind of beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent of any of claims 1 or 2 or claim 3-8 Beach saline land cotton field is being repaired in the beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent that any one preparation method is prepared Application in soil ecology.
10. application according to claim 9, it is characterised in that beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent dosage It is 60-250kg/ mus.
11. application according to claim 9, which is characterized in that the poor, nutrition for fertility of beach saline land continuous cropping Cotton Soil Obstacle is heavy, soil-borne disease is heavy, the soil more than allelochemical.
CN201610301552.5A 2016-05-06 2016-05-06 A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof Expired - Fee Related CN105964680B (en)

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