CN105964680A - Coastal saline continuous cropping cotton soil ecological restoration agent and preparing method and application thereof - Google Patents
Coastal saline continuous cropping cotton soil ecological restoration agent and preparing method and application thereof Download PDFInfo
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- CN105964680A CN105964680A CN201610301552.5A CN201610301552A CN105964680A CN 105964680 A CN105964680 A CN 105964680A CN 201610301552 A CN201610301552 A CN 201610301552A CN 105964680 A CN105964680 A CN 105964680A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The invention relates to a coastal saline continuous cropping cotton soil ecological restoration agent. The coastal saline continuous cropping cotton soil ecological restoration agent comprises, by weight, 2 parts to 200 parts of a trichoderma microbial agent, 5 parts to 300 parts of fulvic acid potassium and 10 parts to 900 parts of other organic matter attached fertilizer. The agent is applied to repairing of coastal saline cotton field soil ecology. Forming of soil water stability aggregates can be promoted, the soil structure is improved, building and maintaining of beneficial microorganism communities in soil are promoted, plant root systems are protected, the agent contends against germs, and plant disease resistance is improved; and new nutrition chains are scientifically combined, plant needs are comprehensively balanced, the higher complexing power of the agent is utilized, absorption and translocation of plant microelements are improved, absorption of crops for nitrogen, phosphorus, potassium and microelements is promoted, the fertilizer utilization rate is improved, adverse factors of poor soil fertilizer, heavy nutritional disturbance, heavy soil-borne diseases and the like existing in the coastal saline cotton field are effectively overcome and solved, and technical supporting is provided for sustainable efficient utilization of the existing cotton field.
Description
Technical field
The invention belongs to soil ecology renovation agent technical field, be specifically related to a kind of beach saline land continuous cropping cotton soil raw
State renovation agent and preparation method and application.
Background technology
China's salt-soda soil resource is the abundantest, all have in main cotton growing area scale not wait salt-soda soil resource, all in all,
The potentiality that development alkaline land cotton produces are the biggest.Shandong is one of province that salt-soda soil, whole nation resource is the abundantest, beach saline land
It is mainly distributed on south, Bohai Sea Gulf bank, the Huanghe delta and Laizhou Bay Coastline, the gross area about 800khm2, after having opened up wasteland or having improved
Plant cotton field, the salt-soda soil about 300khm of cotton2.Due to reasons such as inwelling, soil transpirations, cause beach saline land cotton field saliferous
Amount continues the most higher, cotton grower plantation salt tolerant crop Cotton Gossypii only the most year after year, and soil fertility is poor, nutrition to cause beach saline land cotton field to exist
Obstacle weight, soil-borne disease many unfavorable factors such as heavily, seriously govern the raising of the coastal saline soil cotton level of production.Therefore,
On the one hand develop the preferably cotton saline-alkali wasteland resource expansion in this district and plant cotton area, on the other hand improved now by soil ecology reparation
There are yield and the benefit of beach saline land continuous cropping cotton field cotton, Significance of Sustainable Development weight is produced for Shandong or even national cotton
Greatly.
About developing new saline-alkali wasteland resource, domestic and international researcher at systematic study soil, water body, plant
Proposed on the basis of, weather warping pressure alkali, plough deeply nest salt, pressure sand alkali resistant, green manure turn over and polymer chemistry modifying agent etc.
Method, but the salination mainly for coastal saline-alkali soil of these methods and the soil swelling-shrinkage of meta-alkali, to existing coastal saline-alkali
Continuous cropping cotton cotton field, ground exists that soil fertility is poor, the overcoming and solution of malnutrition weight, soil-borne disease many unfavorable factors such as heavily
Effect is the most notable.
CN 104152149 A disclose a kind of containing rhizopus stolonifer fermentation liquid 20-40, Trichoderma Viride liquid 20-40,
Water-soluble chitosan 1.5-2.5, the Multifunction compound biological soil improver of potassium fulvate 1.5-4.5.This application is soil
The secondary reclamation of salinep-alkali soil in earth top layer, be improve soil pH value, improvement acidified soil, harden soil and secondary salinization soil；
Activating soil nutrient, discharges soil fertility, is not particularly suited for new saline-alkali wasteland improvement.
CN101717646A discloses a kind of containing sheep stool, potassium fulvate, Kang Dibao, manganese sulfate, zinc sulfate, Trichoderma spp., fly
Maggot powder, the soil conditioner preventing and treating take-all of leftover bits and pieces.It is primarily directed to newlyexploited land soil depletion and Semen Tritici aestivi total eclipse
Sick there is serious problem, reach to regulate Soil structure, increase soil fertility, strengthen crop disease-resistant ability, reduce Semen Tritici aestivi total eclipse
Pathogenetic effect, with to alkaline land improving not being a thinking, also cannot provide technology to enlighten.
Summary of the invention
The present invention is directed to beach saline land continuous cropping cotton cotton field present in above prior art and have that soil fertility is poor, battalion
Support obstacle weight, the problem of soil-borne disease many unfavorable factors such as heavily, it is provided that it is water-soluble that one can obviously reduce coast saline soil
Property salt content, reduce soil pH value, induction crop produces stress tolerance to the soil salinization, promotes root system development and the product of crop
The beach saline land continuous cropping cotton soil ecology renovation agent that amount is formed.
Present invention also offers the preparation method of described beach saline land continuous cropping cotton soil ecology renovation agent.
Present invention also offers the application of described beach saline land continuous cropping cotton soil ecology renovation agent.
The present invention is achieved by the following measures:
A kind of beach saline land continuous cropping cotton soil ecology renovation agent, including following component, is weight portion:
Trichoderma spp. microbial bacterial agent 2-200 part, potassium fulvate 5-300 part, other organic additional fertile 10-900 parts；
Effective viable bacteria of described Trichoderma spp. microbial bacterial agent is Trichoderma spp. (Trichoderma sp.) T-15580, culture presevation
Number: CGMCC No.12162, spore content 2-50 × 108cfu/g；
Described potassium fulvate is the product of a kind of commercialization, extracts certain yellow humic acid and oxidation from humic acids
Potassium is made, and outward appearance is chocolate brown powder.Main component: fulvic acid content (in terms of butt) % >=50%, full nitrogen (N) content (with
Butt meter) % >=3.0%, full phosphorus (P) content (in terms of butt) % >=0.4%, full potassium (K02) content (in terms of butt) % >=
11.7%, PH 5.7.
Described beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent, preferred feedstock weight proportion is as follows:
Spore content 6-36 × 108Cfu/g (preferred 6-12 × 108cfu/g、12-36×108Cfu/g) Trichoderma spp. is micro-
Bacteria agent 20-100 part, preferred 20-50 part, 50-100 part, potassium fulvate 80-300 part, preferred 80-150 part,
150-300 part, fertilizer adjuvant 700-900 part, preferred 700-800 part, 800-900 part.
A kind of beach saline land continuous cropping cotton soil ecology renovation agent and preparation method thereof, step is as follows:
(1) take strain Trichoderma spp. T-15580, through actication of culture, seed culture, amplification culture obtain spore content 2-50 ×
108The Trichoderma spp. microbial bacterial agent of cfu/g；
(2) by after Trichoderma spp. microbial bacterial agent and potassium fulvate, the mixing of fertilizer adjuvant, beach saline land Cotton Soil is obtained
Restoration of the ecosystem agent.
Actication of culture condition in described step (1) is: cultivate 70-90h at 26 DEG C-28 DEG C, and culture medium is that PDA cultivates
Base；
Seed culture condition is: 25 DEG C-27 DEG C, shaken cultivation 36-48h under the conditions of 100-180r/min, culture medium is
PD culture medium；
Amplification culture incubation step is: stir culture 36-48h, liquid under the conditions of 24 DEG C-26 DEG C, in fluid medium
Culture medium is Trichoderma spp. microbial liquid culture medium；It is forwarded in solid medium, then under the conditions of 22 DEG C-28 DEG C, solid culture
Cultivating 70-100h in base, solid medium is Trichoderma spp. microbial solid culture medium.
Above-mentioned Trichoderma spp. microbial liquid nutrient media components is: 45-55g Semen Maydis powder, 5-8g bean cake powder, add water to 1000ml,
Sterilizing, filtration.
Above-mentioned Trichoderma spp. microbial solid nutrient media components is: use Testa Tritici 60-80wt%, cotton seed hulls 20-40wt%；Or
Person, Testa Tritici 40-50wt%, rice husk 10-25wt%, corn straw 20-30wt%, corn cob 10-25wt%.
Other culture medium are culture medium commonly used in the art, such as:
PDA culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar powder 15g, water 1000mL；
PD culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, water 1000mL；
Above-mentioned beach saline land continuous cropping cotton soil ecology renovation agent is in repairing beach saline land Cotton Soil ecology
Application, preferable amount is 60-250kg/ mu.
Beneficial effect
1, beach saline land continuous cropping Cotton Soil restoration of the ecosystem agent of the present invention can be salinized soil continuous cropping obstacle soil
Source of life, the food chain that scientific combination is new, overall balance plant demand are provided.The restoration of the ecosystem agent of this soil is except the Huang of high-load
Outside rotten acid, also (oligomeric rich in almost all aminoacid required in growing process, nitrogen, phosphorus, potassium, multiple enzyme, saccharide
Sugar, fructose etc.), protein, nucleic acid, humic acid and the nutrition such as VC, VE and substantial amounts of vitamin B group, be a kind of green
Bioremediation agents；
2, beach saline land continuous cropping cotton soil ecology renovation agent of the present invention can be the strong salinized soil soil of the new reclamation of wasteland
Structural development provides fabricant, and Trichoderma spp. microorganism can promote the formation of soil w ater-stable aggregates, improve Soil structure, promote
The foundation of soil beneficial microbe group and holding, improve biological activity of soil, Trichoderma spp. microorganism as decomposer in soil
Degraded macromolecular substances effect is obvious, and the plant and animal residues in soil can be degraded to small-molecule substance, both by secretion hydrolytic enzyme
Cleaning of beach saline land Cotton Soil ecological environment, enrich again soil nutrient, moreover it is possible to activated mineral, improve soil, improve
Soil productivity, improves the quality of soil, provides technical support for efficiently utilizing of existing cotton field；
3, to can obviously reduce strong coast saline soil water-soluble in beach saline land Cotton Soil restoration of the ecosystem agent of the present invention
Property salt content, reduce soil pH value, induction crop produces stress tolerance to the soil salinization, promotes root system development and the product of crop
Amount is formed；
4, the Trichoderma spp. microorganism of beach saline land Cotton Soil restoration of the ecosystem agent of the present invention promotes the micro-life of beneficial rhizosphere
Thing grows, and protects root system of plant, contends with cotton verticillium wilt, Sclerotium rolfsii, and then suppression crop soil-borne disease bacteria growing, improves
Disease resistance of plant, overcomes continuous cropping obstacle, promotes plant growing, improves crop yield and quality；
5, beach saline land continuous cropping cotton soil ecology renovation agent Trichoderma spp. of the present invention and fulvic acid mutual reciprocity and mutual benefit, yellow humic
Acid can become the nutrient substance of Trichoderma spp. growth under certain conditions, and the colonization ability increasing Trichoderma spp. improves Trichoderma spp. proliferative amount, wood
Mould metabolite provides phytohormone, hydrolytic enzyme and biochemical activity material for fulvic acid, improves the activity of fulvic acid further, increases
Strong fulvic acid effect；
6, beach saline land continuous cropping cotton soil ecology renovation agent complexing power of the present invention is strong, improves minimum plant unit
The absorption of element and operating.One of this soil restoration of the ecosystem agent component potassium fulvate is due to total amino acids and carboxyl, hydroxyl isoreactivity
Group content is higher, has again oxygen-containing functional group, and structure exists many organic chelating positions and complexation position, these coordinating group energy
Many trace element generation complexations such as such as calcium, magnesium, sulfur, ferrum, manganese, copper, zinc, boron etc. with indissoluble or chelating reaction, also can simultaneously
Rising on his site with phosphorus element generation complex reaction, thus formed with fulvic acid molecule for intermediary's carrier, coordinating simultaneously, promote to plant
Thing root system or blade face, to the absorption in vivo of trace element and phosphorus, operating, not only avoid the trace element direct contact with phosphorus
And cause being passivated each other inactivation, and serve positive balanced action, thus improve both utilization rates；
7, beach saline land Cotton Soil restoration of the ecosystem agent of the present invention can promote that crop is to nitrogen, phosphorus, potassium and trace unit
The absorption of element, improves utilization rate of fertilizer, has the effect of buffering fertilizer efficiency, when soil and fertilizer nutrient is too much, this renovation agent simultaneously
In microorganism absorb abundant inorganic nutrients and be housed in thalline, make rhizosphere nutrient density be gradually lowered, when soil nutrient not
During foot, along with the death of microorganism, the nutrient absorbed by thalline the most gradually discharges, and absorbs for crop.
Culture presevation information
The preservation time: on February 25th, 2016,
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Deposit number: CGMCC No.12162,
Depositary institution address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postal
Coding: 100101 Classification And Nomenclatures: Trichoderma spp. Trichoderma sp..
Detailed description of the invention
Below in conjunction with example, technical scheme is described in further detail, but embodiments of the present invention are not limited to
This.
Potassium fulvate described in embodiment is purchased from Shandong Creation Hhumic Acid Science and Technology Co., Ltd, and Cotton Gossypii LMY-36 is purchased from
Shandong Cotton Research Center.
Acquisition, screening and the preservation of embodiment 1 bacterial strain
Culture medium:
PDA culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar 15g, water 1000mL；
Martin (Martin) culture medium: peptone 5g, glucose 10g, KH2PO41g, MgSO4.7H2O 0.5g, agar
20g, rose-bengal (1%) 3.3mL, streptomycin 33 milligrams, pH 7.0-7.2, water 1000mL.
The acquisition of bacterial strain:
In slight alkaline land cotton yellow droop morbidity field, around cotton of morbidity and a cotton rhizosphere of not falling ill, remove 1-
5cm topsoil, takes topsoil soils loading paper bag and takes back laboratory separation.Pedotheque is made a series of concentration, takes the denseest
Degree diluent is coated on Martin's culture medium flat plate, cultivates 3-4d for 26 DEG C-28 DEG C, and picking form is transferred like single bacterium colony of trichoderma
In PDA culture medium, it is purified cultivation, numbers after inspection is initially identified as Trichoderma spp., and slant culture cryopreservation.
Pass through above-mentioned steps, it is thus achieved that the bacterial strain of a lot of pure cultures, series wood trichoderma strain is carried out the speed of growth, sporulation quantity etc.
Shape matching, primary dcreening operation obtains preferable 2 bacterial strains and cultivates a large amount of reesei spores of acquisition respectively, makes 107-108Cfu/mL spore hangs
Supernatant liquid, is separately added into 0.01mol/L sodium nitrite solution mutation 20min, and its fatality rate, at 70%-80%, takes the most respectively
0.1mL is spread evenly across on PDA plate, place under 20W uviol lamp 20cm irradiate 3min, its fatality rate at 80%-90%, 25
DEG C cultivate 12h-24h, monospore picking sprout single thalline cultivate on PDA plate, further choose normal growth mutant bacteria
In strain successive transfer culture 5-10 generation, after bacterial strain is stable, series mutagenic strain and former Strain comparison, primary dcreening operation obtains more excellent new strains.
Being grown in post-directed training base by dominant strain, the culture medium of salt concentration 6 ‰-10 ‰ obtains salt-enduring strain, with
Cotton yellow Sclerotium rolfsii (verticillium dahliae Verticillium dahliae；Cotton wilting Fusarium spp. Fusarium
Vasinfectum) opposite culture in PDA culture medium, with starting strain for comparison, obtains Antagonism bigger than starting strain has
The new dissociant improved, by a wherein named T-15580 of strain.Its increment, sporulation quantity and to pathogenic bacteria inhibitory action, cover
The indexs such as lid effect improve 4.5%, 10.2%, 41.1%, 11.4% than starting strain respectively.
The morphological characteristic of bacterial strain:
Trichoderma spp. (Trichoderma spp.) colony growth is fast, and aging cultivation colony colour is green, has fragrance, sporophore
Cong Shu arranges in the form of a ring.Secondary branch is single or be that one group of wide-angle is stretched out with 2-3, and branch usually bends or in wavy, bottle
Hanging contracting in metulae portion, centre is expanded, straight or bending, and under ultimate bottle obstructs, 2-3 bottle stalk arranges with false wheel mode, the most single or
Stretch out in pairs.Conidium is subsphaeroidal, and size is 3.4-4.3 × 2.6-3.4um.
Embodiment 2 soil-repairing agent is to strong salinized soil salt reduction alkali and improves soil biomass effect
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each ingredients weight parts is as follows:
Trichoderma spp. microbial bacterial agent 20 parts, potassium fulvate 80 parts, farm manure 900 parts；
Above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent is prepared as follows:
(1) preparation of Trichoderma spp. microbial bacterial agent:
Actication of culture
T-15580 is that oneself laboratory separates, and obtains through the various growth limit conditional filterings such as high salt, and with currently
The T22 strain suppression pathogen growth comparative test that bio-control factors effect is strong.
Take the Trichoderma spp. T-15580 that culture presevation number is CGMCC No.12162, be inoculated in 26 DEG C of cultivations in PDA culture medium
72h, obtains activating Trichoderma spp. T-15580；
PDA culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, agar powder 15g, water 1000mL.
Seed culture
The sterilized water dilution of activation Trichoderma spp. T-15580 spore is accessed the 250mL triangular flask kind equipped with 50mLPD culture medium,
26 DEG C, 180r/min, shaken cultivation 72h, obtain seed Trichoderma spp. T-15580；
PD culture medium: Rhizoma Solani tuber osi 200g, glucose 20g, water 1000mL.
Amplification culture
I 100mL seed Trichoderma spp. T-15580 is inoculated in 16 liters of fermentations equipped with 7 liters of Trichoderma spp. microbial liquid culture medium by ()
In tank, stir culture 40h at 26 DEG C, obtain Trichoderma spp. T-15580 liquid；
Trichoderma spp. microbial liquid culture medium: 47.5g Semen Maydis powder, 7.5g bean cake powder, add water to 1000mL, sterilizing, filtration.
(ii) with the ratio of 5wt%, Trichoderma spp. T-15580 liquid being moved into Trichoderma spp. microbial solid culture medium, tray is placed,
Space relative humidity 70%-90%, well-ventilated, periodically turn over, cultivate 96h for 26 DEG C, obtain reesei spores content 36 ×
108The Trichoderma spp. microbial bacterial agent of cfu/g.
Trichoderma spp. microbial solid culture medium: after using 80wt% Testa Tritici to mix with 20wt% cotton seed hulls, sterilizing.
(2), after Trichoderma spp. microbial bacterial agent prepared for step (1), potassium fulvate, farm manure being mixed by weight, shore is obtained
Sea salt-soda soil Cotton Soil restoration of the ecosystem agent.
Test example
Experiment plot: Dongying city has opened up wasteland Kenli County strong salinized soil continuous cropping ground, strand
Because this plot is to have opened up wasteland plot, before spring ploughing, field vegetation dry weight is less than every 667 square metres of 100kg, by this ground
Block is divided into soil-repairing agent group and two pieces of plot of matched group:
Modifying agent group uses coast saline soil restoration of the ecosystem agent by 250kg/ mu amount, irrigation of turning over, real-time sowing cotton,
Cultivation management presses conventional tillage management operation；
Matched group does not use coast saline soil restoration of the ecosystem agent, and other are with renovation agent group.
Test data and experimental result:
Table 1 soil-repairing agent salinized soil strong on strand Cotton Gossypii emerges and the impact of yield
Experimental result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention makes the emergence rate of Cotton Gossypii improve
32.7%, planting percent improve 48.9%, solve strong alkaline land cotton emerge difficult and vertical Seedling difficulty problem, promote Cotton Gossypii normal season
Joint is bloomed and blow-of-cottons, and cotton boll blow-of-cottons are concentrated, and increases substantially than flower before comparison frost, and before frost, flower rate improves 409.7%, cotton quality
Improving, yield increases substantially.
The regulating effect of table 2 soil-repairing agent salinized soil strong to strand continuous cropping cotton field microorganism
The regulating effect of table 3 soil-repairing agent salinized soil strong to strand continuous cropping cotton field microbial population
| Process | Bacterial species | Actinomycetes | Fungal species | The soil-borne disease bacterium such as fusarium |
| Renovation agent group | More than 4 kinds | There is some | More than 3 kinds | It is not separated to |
| Matched group | Single | Do not turn out | Single | 3 kinds |
Result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves strong salinized soil, increases substantially soil
Earth micro organism quantity, the beneficial microbes such as microbe species increases, actinomycetes increase, and optimize structure of soil microbial community, soil
In earth, the harmful microorganism kind such as fusarium and quantity are substantially reduced.
Table 4 soil ecology renovation agent salinized soil strong to strand continuous cropping cotton field physicochemical character improvement situation
Result shows: the coast saline soil restoration of the ecosystem agent of the present invention physics and chemistry to the strong salinized soil in strand, Dongying City Kenli County
Character has obvious improved effect.Improve soil organic matter content, significantly reduce coast saline soil water soluble salt and contain
Amount, reduces soil pH value, significantly improves soil w ater-stable aggregates content.
Embodiment 3 soil-repairing agent alignment degree salinized soil improves soil vitality and output of cotton effect
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each ingredients weight parts is as follows:
Trichoderma spp. microbial bacterial agent 50 parts, potassium fulvate 150 parts, 800 parts of poultry muck；
As described in Example 1, difference is above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent preparation method:
Trichoderma spp. microbial bacterial agent is reesei spores content 12 × 108The Trichoderma spp. microorganism of cfu/g；
After Trichoderma spp. microbial bacterial agent, potassium fulvate, poultry muck being mixed by weight, obtain beach saline land cotton field soil
Earth restoration of the ecosystem agent.
Test example
Experiment plot: Dongying city has opened up wasteland Kenli County moderate salinized soil continuous cropping ground, strand
Because this plot is to have opened up wasteland plot, before spring ploughing, field vegetation dry weight is less than every 667 square metres of 100kg, by this ground
Block is divided into soil-repairing agent group and two pieces of plot of matched group:
Modifying agent group uses coast saline soil restoration of the ecosystem agent by 150kg/ mu amount, irrigation of turning over, real-time sowing cotton,
Cultivation management presses conventional tillage management operation；
Matched group does not use coast saline soil restoration of the ecosystem agent, and other are with renovation agent group.
Test data and experimental result:
The impact on strand moderate salinized soil Developmental of Cotton of table 5 soil-repairing agent
Experimental result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention promotes cotton growth, plant height
Improving, the renovation agent lasts phase is long, regulates Developmental of Cotton, and Cotton Gossypii blooms concentrations, and cotton boll blow-of-cottons are concentrated, and spends rate big before frost
Amplitude improves, and cotton quality improves, and yield increases substantially.
Table 6 soil-repairing agent regulating effect to moderate salinized soil continuous cropping cotton field, strand microbial population
Experimental result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves soil microbe quantity, puts
The soil-borne disease bacterium quantity such as the beneficial microbes such as line bacterium, nitrogen-fixing bacteria, photosynthetic bacteria increase, fusarium decline.
Table 8 soil-repairing agent regulating effect to strand moderate salinized soil continuous cropping Cotton Soil hydrolytic enzyme activities
Experimental result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves moderate salinized soil continuous cropping soil
Loam Soil enzyme, adds the biochemical reaction ability of soil, improves soil vitality, increases soil soluble nutrition, promotes
Cotton growth and growth, improve Resistance Strain of Cotton adverse circumstance ability.
Slight salinized soil is prevented and treated cotton yellow droop effect by embodiment 4 soil-repairing agent
A kind of beach saline land Cotton Soil restoration of the ecosystem agent, each ingredients weight parts is as follows:
Trichoderma spp. microbial bacterial agent 100 parts, potassium fulvate 300 parts, fertilizer 700 parts；
As described in Example 1, difference is above-mentioned beach saline land Cotton Soil restoration of the ecosystem agent preparation method:
Trichoderma spp. microbial bacterial agent is reesei spores content 6 × 108The Trichoderma spp. microorganism of cfu/g；
After Trichoderma spp. microbial bacterial agent, potassium fulvate, fertilizer being mixed by weight, obtain beach saline land Cotton Soil
Restoration of the ecosystem agent.
Test example
Experiment plot: strand, Kenli County, Dongying city slight salinized soil continuous cropping cotton ground
This plot is divided into renovation agent group and two pieces of plot of matched group, and renovation agent group uses beach salty soil by 60kg/ mu
Continuous cropping cotton soil ecology renovation agent, irrigation of turning over, real-time sowing cotton, cultivation management presses conventional tillage management operation；
Matched group does not use coast saline soil restoration of the ecosystem agent, and other are with modifying agent group.
Test data and experimental result:
Table 9 soil-repairing agent is slight to strand salinized soil cotton yellow droop disease-controlling effect
Table 9 result illustrates, beach salty soil continuous cropping cotton soil-repairing agent of the present invention increases substantially the side radical of Cotton Gossypii,
The growth of suppression soil-borne disease, improves the resistance against diseases of Cotton Gossypii, improves output of cotton, improves the income at plantation family.
The regulating effect of salinized soil continuous cropping cotton field microbial population that table 10 soil-repairing agent is slight to strand
Table 10 result shows, beach salty soil continuous cropping cotton soil-repairing agent of the present invention improves soil microbe quantity, changes
Good soil microbial community forms, and the beneficial microbe such as nitrogen-fixing bacteria, photosynthetic bacteria, cellulose-decomposing bacterium increases, reaping hook in soil
The harmful microorganism quantity such as bacterium are substantially reduced.
Salinized soil physicochemical character improvement situation that table 11 soil ecology modifying agent is slight to strand
Table 11 is the testing result that beach salty soil continuous cropping cotton soil-repairing agent of the present invention uses after soil after 78 days, inspection
Mark is accurate: hydrolyzable nitrogen according to People's Republic of China (PRC) agricultural industry criteria NY/T 1848-2010, available phosphorus according to the China people
Republic agricultural industry criteria NY/T 1121.7-2006, available potassium are according to People's Republic of China's forestry industry standard LY/T
1236-1999, water soluble salt according to People's Republic of China (PRC) agricultural industry criteria NY/T 1121.16-2006, pH value according in
China people's republic agricultural industry criteria NY/T 1121.2-2006, water-stable aggregate are according to People's Republic of China's agricultural row
Industry standard NY/T 1121.19-2008.Result illustrates, using of soil-repairing agent, and in soil, solable matter increases, and improves soil
The utilization rate of earth nutrition, beneficially cotton root system absorb, utilize, and promote cotton growth, and soil w ater-stable aggregates increases simultaneously, changes
Kind Soil structure.
Comprehensive above experimental result, coast saline soil restoration of the ecosystem agent of the present invention significantly improves soil microbe quantity,
Improve soil microbial population structure, improve soil organic matter content, improve soil w ater-stable aggregates quantity, reduce Huang
Delta, river coast saline soil water soluble salt content, reduces soil pH value, improves biological activity of soil, higher in salinity
Strong salinized soil significantly improve emergence rate and the planting percent of Cotton Gossypii, the growth of suppression soil-borne disease, inducing cotton is to soil salting
Change and produce stress tolerance and disease resistance, promote root system development and the yield composition of crop.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention are not limited by embodiment
System, the change made, modifies, combines, substitutes, simplifies and all should be under other any spirit without departing from the present invention and principle
Equivalence substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. a beach saline land is punished for being related to Cotton Soil restoration of the ecosystem agent, it is characterised in that raw material weight proportioning is as follows:
Trichoderma spp. microbial bacterial agent 2-200 part, potassium fulvate 5-300 part, fertilizer adjuvant 10-900 part；
Effective viable bacteria of described Trichoderma spp. microbial bacterial agent is Trichoderma spp. T-15580, culture presevation number: CGMCC No.12162, spore
Content 2-50 × 108cfu/g。
Beach saline land the most according to claim 1 is punished for being related to Cotton Soil restoration of the ecosystem agent, it is characterised in that raw material weight
Proportioning is as follows:
Spore content 6-36 × 108Trichoderma spp. microbial bacterial agent 20-100 part of cfu/g, potassium fulvate 80-300 part, fertilizer adjuvant
700-900 part.
3. the beach saline land described in claim 1 or 2 is punished for being related to a preparation method for Cotton Soil restoration of the ecosystem agent, its feature
It is
(1) take strain Trichoderma spp. T-15580, obtain Trichoderma spp. microbial bacterial agent through actication of culture, seed culture, amplification culture；
(2) by after Trichoderma spp. microbial bacterial agent and potassium fulvate, the mixing of fertilizer adjuvant, to obtain final product.
Preparation method the most according to claim 3, it is characterised in that the actication of culture condition in step (1) is: 26 DEG C-
Cultivating 70-90h for 28 DEG C, culture medium is PDA culture medium.
Preparation method the most according to claim 3, it is characterised in that the seed culture condition in step (1) is: 25 DEG C-
27 DEG C, shaken cultivation 36-48h under the conditions of 100-180r/min, culture medium is PD culture medium.
Preparation method the most according to claim 3, it is characterised in that the amplification culture in step (1) is: at 24 DEG C-26 DEG C
Under the conditions of, stir culture 36-48h in fluid medium, fluid medium is Trichoderma spp. microbial liquid culture medium；It is forwarded to solid
In culture medium, then under the conditions of 22 DEG C-28 DEG C, cultivate 70-100h, solid medium is Trichoderma spp. microbial solid culture medium.
Preparation method the most according to claim 6, it is characterised in that Trichoderma spp. microbial liquid nutrient media components described in step
For: 45-55g Semen Maydis powder, 5-8g bean cake powder, add water to 1000mL, sterilizing, filtration.
Preparation method the most according to claim 6, it is characterised in that described Trichoderma spp. microbial solid nutrient media components is:
Testa Tritici 60-80wt%, cotton seed hulls 20-40wt%；
Or, Testa Tritici 40-50wt%, rice husk 10-25wt%, corn straw 20-30wt%, corn cob 10-25wt%.
9. the beach saline land described in a claim 1 or 2 is punished for being related in Cotton Soil restoration of the ecosystem agent or claim 3-8
The beach saline land that any one preparation method prepares is punished for being related to Cotton Soil restoration of the ecosystem agent in reparation beach saline land cotton field
Application in soil ecology.
Application the most according to claim 9, it is characterised in that beach saline land is punished for being related to Cotton Soil restoration of the ecosystem agent consumption
For 60-250kg/ mu.
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