CN107382453A - Soil disinfection regeneration anti obstacle to continuous cropping composition and preparation method and application - Google Patents
Soil disinfection regeneration anti obstacle to continuous cropping composition and preparation method and application Download PDFInfo
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- CN107382453A CN107382453A CN201710538758.4A CN201710538758A CN107382453A CN 107382453 A CN107382453 A CN 107382453A CN 201710538758 A CN201710538758 A CN 201710538758A CN 107382453 A CN107382453 A CN 107382453A
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- 238000009335 monocropping Methods 0.000 title claims abstract description 61
- 239000000203 mixture Substances 0.000 title claims abstract description 40
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 37
- 230000008929 regeneration Effects 0.000 title claims abstract description 27
- 238000011069 regeneration method Methods 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 239000002028 Biomass Substances 0.000 claims abstract description 38
- 244000005700 microbiome Species 0.000 claims abstract description 20
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 16
- 239000004094 surface-active agent Substances 0.000 claims abstract description 16
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 19
- 229910052799 carbon Inorganic materials 0.000 claims description 19
- 239000003795 chemical substances by application Substances 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 13
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- 239000007791 liquid phase Substances 0.000 claims description 8
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- 241000238631 Hexapoda Species 0.000 claims description 7
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- 239000002994 raw material Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 4
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- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 3
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- 238000009923 sugaring Methods 0.000 claims description 3
- KPZTWMNLAFDTGF-UHFFFAOYSA-D trialuminum;potassium;hexahydroxide;disulfate Chemical compound [OH-].[OH-].[OH-].[OH-].[OH-].[OH-].[Al+3].[Al+3].[Al+3].[K+].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O KPZTWMNLAFDTGF-UHFFFAOYSA-D 0.000 claims description 3
- 239000002916 wood waste Substances 0.000 claims description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 2
- -1 alkyl glucoside Chemical class 0.000 claims description 2
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- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 2
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- 230000003213 activating effect Effects 0.000 claims 1
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- 241000233616 Phytophthora capsici Species 0.000 description 9
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- 235000015759 Artemisia selengensis Nutrition 0.000 description 1
- 241001168877 Artemisia selengensis Species 0.000 description 1
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- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 1
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
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- 241000879841 Fusarium oxysporum f. cubense Species 0.000 description 1
- 241001635593 Lisianthius Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 101000905241 Mus musculus Heart- and neural crest derivatives-expressed protein 1 Proteins 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
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- GZUXJHMPEANEGY-UHFFFAOYSA-N bromomethane Chemical compound BrC GZUXJHMPEANEGY-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C5/00—Fertilisers containing other nitrates
- C05C5/02—Fertilisers containing other nitrates containing sodium or potassium nitrate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
- C05D1/02—Manufacture from potassium chloride or sulfate or double or mixed salts thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Soil Sciences (AREA)
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Abstract
The present invention discloses a kind of soil disinfection regeneration anti obstacle to continuous cropping composition and preparation method and application, and said composition includes special microorganism, organic catalysis thing, mineralizer, surfactant and organic biomass;It can accelerate to create soil Strong reducing environments under environment of being sick of, soil-borne pathogen is killed in a short time, change structure of soil microbial community, improve soil pH, eliminate secondary salinization effect, so that regradation of soil to administer continuous cropping as obstacle, can improve per mu yield, the effect in terms of soil is restored, administer soil continuous cropping obstacle is notable.
Description
Technical field
The present invention relates to agricultural sustainable development field, particularly a kind of soil disinfection regeneration anti obstacle to continuous cropping composition and
Its preparation method and application.
Background technology
Continuous cropping is the mode of production that modern age merchandized handling is done something against one's will, and it can make soil degradation and cause to make
Thing dysplasia.Under soil multiple crop index height, continuous cropping serious condition, continuous cropping obstacles have turned into agricultural to be held
One of significant problem of supervention exhibition.
After continuous cropping obstacles refer to same crop or nearly edge continuous cropping, even in the situation of normal cultivation management
Under, also it can produce yield reduction, the phenomenon that quality deteriorates, bearing status is deteriorated.Its mainly as caused by fissioning soil degradation,
4 aspects can be specifically summarized as:1. soil-borne disease harm aggravates(As Fusarium oxysporum cucumber specialized form, Fusarium oxysporum celery are special
Change type, Fusarium oxysporum Lisianthus specialized form, Ralstonia solanacearum, phytophthora blight of pepper);2. soil secondary salinization, acidifying, plate
Knot;3. soil nutrient is unbalance;4. the accumulation to plant itself harmful substance from plant(Such as phenolic acid class and saponins allelopathic thing
Matter).
The continuous cropping obstacle prevention and controls of main flow mainly have following several at present:1. crop rotation, intercropping;2. grafting or selection
Disease-resistant variety;3. chemical fumigation sterilizing, high-temperature sterilization;4. washing salinity by irrigation, quick lime sweetening of the soil acidity, soil removal and replacement;5. apply life
Organic fertilizer, inoculation are beneficial to functional microorganism.
Above-mentioned several methods can mitigate or delay to a certain extent the generation of continuous cropping obstacle, but office be present
It is sex-limited.
By taking crop rotation as an example, under the restriction of the factor such as actual planting conditions and the market demand, the increase of soil multiple crop index is not
Must for it then, taking turns preventing and treating continuous cropping obstacle of opposing has certain effect, but the economic benefits such as local market and brand can be made
Into influence, and with the extension of Planting Years, it can also trigger crop growth obstacle to some extent.From grafting or disease-resistant product
Kind can prevent and treat the infringement of a part of soil-borne pathogen, but cost certainly will increase, and such as run into serious soil-borne disease insect pest or soil
Serious secondary salinization itself, acidifying, hardened etc., can not reach preferable prevention effect.For part soil-borne pathogen into
The soil continuous cropping obstacle of cause, chemical fumigation effect is preferable, but because of serious harm of the chemicals to ozone and health, one
Divide disinfecting drug(Such as bromomethane)Forbidded strictly.A part can be also killed using the sterilization of summer sunshine high temperature close plastic shed to deposit
It is pathogen, worm's ovum and the weed seed of upper soll layer, but serious continuous cropping obstacle is still imitated without preferably preventing and treating
Fruit.The method that soil pH is adjusted by washing salinity by irrigation and quick lime also hinders just for continuous cropping caused by soil physico-chemical property degeneration
Hinder, but cure the symptoms, not the disease, and can make it that loss of soil nutrient is serious, soil is endangered in itself larger;Soil replacement method cost is higher, greatly
Scale is promoted obvious unrealistic.At present, using the really relatively method of safety and environmental protection of biological organic fertilizer, inoculation biocontrol microorganisms,
But generally only rise alleviate soil continuous cropping obstacle function, continuous cropping obstacle once occur, be difficult to it is effective in a short time
Improve degenerated soil, suppress pathogen amount reproduction formation community superiority.
Therefore, in terms of the preventing and treating of continuous cropping obstacle, there is an urgent need to seek a kind of effective, comprehensive preventing and treating product and
Application technology.
The content of the invention
It is an object of the invention to overcome killing soil-borne pathogen in existing continuous cropping obstacle prevention and controls while kill beneficial
Bacterium, soil physical and chemical property, effectively toxic side effect, the preventing and treating time not drawback such as long, unstable can not be improved, there is provided one kind has
Soil-borne pathogen is quickly killed in a short time, changes structure of soil microbial community, is improved soil pH and is eliminated secondary salinization
Soil disinfection regeneration anti obstacle to continuous cropping composition and preparation method thereof, said composition and its lonely application technology can not only be killed
Disease fungus, bacterium and nematode, and soil physico-chemical property is can effectively improve, increase soil fertility, suppress weed growth.The present invention
It is realized in:
A kind of soil disinfection regenerates anti obstacle to continuous cropping composition, in said composition, by percentage to the quality:Special microorganism accounts for 0-
5%, organic catalysis thing accounts for 0-1%, and mineralizer accounts for 0-15%, and surfactant accounts for 0-1%, and 100% is supplemented to organic biomass.
Further, in soil disinfection regeneration anti obstacle to continuous cropping composition provided by the invention, it is calculated in mass percent, it is special
Microorganism accounts for 1-2%, and organic catalysis thing accounts for 0.1-0.4%, and mineralizer accounts for 1-2%, and surfactant accounts for 0.1-0.3%, with organic life
Material is supplemented to 100%.
Further, in soil disinfection regeneration anti obstacle to continuous cropping composition provided by the invention, organic biomass is easily decomposition
High reducing substances, including solid phase organic biomass and liquid phase organic biomass.Wherein, the solid phase organic biomass includes
Cellulose, starch, wood wastes(Such as dry branches and fallen leaves, wood chip, pine bark etc.), stalk(Such as rice, wheat stalk), livestock and poultry
Excrement(Such as dried poultrymanure), insect excrement(Such as silkworm excrement), sugaring crop waste residue(Such as manioc waste), plant and animal residues fermentation
One or more in thing, livestock manure fermented thing or insect manure fermentation thing, preferably(By percentage to the quality)Total organic carbon
TOC>20%, cellulose carbon COC>10%, Easily oxidized carbon EOC>7%, Ca, Mg, Si total amount>5% solid phase organic biomass;
The liquid phase organic biomass includes the one or more in alcohol, glucose, sucrose, maltose or molasses, preferably(With
Mass percent meter)Total organic carbon TOC>20%, Easily oxidized carbon>15% liquid phase organic biomass.
Further, in soil disinfection regeneration anti obstacle to continuous cropping composition provided by the invention, special microorganism is organic to urge
Agent, mineralizer and surfactant act as promoting high reproducibility organic biomass fast decoupled;Wherein, it is described special
Microorganism includes but is not limited to feature saccharomycete, bacillus, special fungi(Such as Trichoderma Trichoderma and viscous broom
Mould Gliocladium);Mineralizer includes but is not limited to biological carbon and acid, basic soil mineralizer(Such as glacial acetic acid, nitre
Stone, alunite);Surfactant includes but is not limited to nonionic/cloudy from bonding agent, nonionic penetrant, cation permeability
Agent and anion-permeable membrane agent(Such as stearic acid, quaternary ammonium compound, lecithin, alkyl glucoside(APG), fatty glyceride).
Further, organic biomass provided by the present invention is rice straw.
A kind of preparation method of soil disinfection regeneration anti obstacle to continuous cropping composition of the present invention, it is comprised the following steps that:
A:Select and collect labile high reproducibility organic biomass
According to soil types and crop choice material, high reproducibility organic biomass must easily decompose high reproducibility, including
But straw is not limited to, agricultural solid residue and plant and animal fermented product, further comparatively ideal labile height
Reproducibility organic biomass is rice straw, and its optimization technique standard is:It is calculated in mass percent, moisture<20%;Total organic carbon
TOC>20%;Cellulose carbon>10%;Ca, Mg and Si total amount>5%, fineness 0.1-2mm.
In addition, ratio of the high reproducibility organic biomass of the present invention in continuous cropping reconstituted product may be up to 100%;
B:Select the dispensings such as special microorganism, organic catalysis thing, mineralizer and surfactant
Choose special microorganism in proportion, organic catalyst, mineralizer and surfactant act as promoting high reproducibility
Organic biomass fast decoupled, wherein special microorganism include but is not limited to feature saccharomycete, bacillus, special fungi
Deng;Organic catalysis thing includes but is not limited to cellulase or other biological matter catabolic enzyme;Mineralizer includes but is not limited to acidity,
Alkalescence and biological carbon, soil mineralization thing etc.;Surfactant includes but is not limited to nonionic/cloudy from bonding agent, nonionic infiltration
Agent, cation permeability agent and anion-permeable membrane agent.
C:Make soil renewal agent
Main material A and dispensing B is chosen according to crop and continuous cropping obstacle situation in actual production, main material A includes plant straw
Stalk, comparatively ideal is rice straw.Dispensing B includes but is not limited to special microorganism, organic catalysis thing, mineralizer and surface-active
One or more in agent, can be liquid or solid form.Crushing is carried out according to matched somebody with somebody raw material to be blended(It is crushed to particle diameter 2mm-
100mm).Wherein, some materials need nanoscale to crush(Such as mineralizer and organic catalysis thing, particle diameter 100nm-400nm is crushed to)
To reach optimum efficiency, it is sufficiently mixed(It is 100-200 revs/min to mix rotating speed)Afterwards, that is, obtain the soil disinfection and regenerate anti-continuous cropping
Obstacle composition;Wherein, using solid phase organic biomass product upon mixing, also need drying(70-100 DEG C of dry 10-
60min), cooling(Natural cooling 10-60 minutes at room temperature)Packaging is carried out again obtains finished product.
Application of the soil disinfection regeneration anti obstacle to continuous cropping composition of the present invention in continuous cropping obstacle is improved, its specific step
Suddenly it is:
A:Soil disinfection regenerates anti obstacle to continuous cropping composition(15 tons/hectare)With crop Soil tillage(20-30cm)It is well mixed;
B:Poured water after mixing, waterflooding(Irrigate to maximum field capacity)Or overlay film creates anaerobic condition, created for course of reaction
Condition;
C:It is sick of and handles 2-45 days, the standard for determining processing effective time is oxidation-reduction potential(Less than -60mV);
D:Film is taken off after reaction completely and completes improvement to continuous cropping soil.
The present invention can accelerate to create soil Strong reducing environments under environment of being sick of, and kill soil-borne pathogen in a short time, change
Become structure of soil microbial community, improve soil pH, eliminate secondary salinization effect, so that regradation of soil is to administer crop
Continuous cropping is obstacle.
Compared with prior art, for the present invention without any waste of raw materials, production cost is low, and may be directly applied to existing have
Machine production technique for fertilizer.
The present invention adapts to high intensity plantation, and green, significant effect being capable of comprehensive improved soil.At the present invention
Soil weeds can be killed after reason and soil-borne disease bacterium includes but is not limited to Fusarium, pythium spp, phytophthora, Raul Salmonella and line
Worm, insect killing effect reach 90-99%;Per mu yield can be improved, the effect in terms of soil is restored, administer soil continuous cropping obstacle is notable.
Brief description of the drawings
Fig. 1 is embodiment soil pH testing result schematic diagram;
Fig. 2 is embodiment product soil EC testing result schematic diagrames.
Embodiment
Involved raw material in embodiment:
Saccharomycete, bacillus amyloliquefaciens mend Eco-fertilizer industry Co., Ltd purchased from Jiangsu day;
Trichoderma, gliocladium germ and cellulase are purchased from Ke Li Eco Science Technologies Co., Ltd.
Embodiment 1
By wheat stalk and silkworm excrement(Equal powder particle diameter 2mm-100mm)By 50:50 part by weight mixing is used as labile height
Reproducibility organic biomass, and the labile high reproducibility organic biomass is crushed according to 100% proportion by thorough
(It is crushed to particle diameter 2mm-100mm)Mixing, mixing rotating speed are 100-200 revs/min, 70-100 DEG C of dry 10-60min, so
Natural cooling 10-60 minutes, acquisition soil disinfection regenerate anti obstacle to continuous cropping composition at room temperature afterwards.
The technical indicator of the organic biomass is:Moisture:19%;Total organic carbon TOC:31%;Cellulose carbon COC:11%;Easily
Carbonoxide EOC:9%;Ca, Mg, Si total amount:7%.
Embodiment 2
(1)By saccharomycete, bacillus(Bacillus amyloliquefaciens), fungi(Trichoderma Trichoderma)By 30:30:40
Part by weight mixing is used as special microorganism;
Using cellulase as organic catalysis thing;
Using charcoal as mineralizer;
By anion-permeable membrane agent(Stearic acid)With nonionic penetrant(Fatty glyceride)By 50:50 weight is than mixing conduct
Surfactant;
By rice straw and clover stalk(Equal powder particle diameter 2mm-100mm)By 50:50 part by weight mixing is as easily decomposition
High reproducibility organic biomass;The technical indicator of organic biomass is:Moisture:19%;Total organic carbon TOC:32%;Cellulose carbon
COC:12%;Easily oxidized carbon EOC:10%;Ca, Mg, Si total amount:7%.
(2)By percentage to the quality, special microorganism 1%, organic catalysis thing 0.1%, soil mineralization thing 1%, surfactant
0.1%, surplus is organic biomass;
Above-mentioned raw materials are mixed(Rotating speed is 100-200 revs/min), 70-100 DEG C of dry 60min, room temperature cooling 10-60 minutes, i.e.,
Soil disinfection regeneration anti obstacle to continuous cropping composition is made.
Embodiment 3
(1)By feature saccharomycete, bacillus(Bacillus amyloliquefaciens), fungi(Gliocladium germ Gliocladium)Press
40:40:20 part by weight mixing is used as special microorganism;
Cellulase is crushed to particle diameter<400nm, as organic catalysis thing;
By acid mineralizer(Glacial acetic acid)With alkaline mineralizer(Alunite)By 50:50 weight is crushed to particle diameter than mixing
100nm-400nm, as mineralizer;
By anion-permeable membrane agent(Neopelex), nonionic penetrant(Fatty glyceride)By 60:40 weight
Surfactant is used as than mixing;
It is using molasses as labile high reproducibility organic biomass, the technical indicator of organic biomass:Moisture<29%;Containing sugar
Amount>42%;Thick protein>2.5%;PH value 5-7.5;Viscosity is 3000~8000 centipoises at 20 DEG C.
(2)By percentage to the quality, by 2% weight than special microorganism, 0.4% weight than organic catalysis thing, 2% weight
Measure the mineralizer of ratio, 0.3% weight than surfactant, complement to 100% with labile high reproducibility organic biomass;
Above-mentioned raw materials are mixed(Rotating speed is 100-200 revs/min), 70-100 DEG C of dry 60min, room temperature cooling 10-60 minutes, i.e.,
Soil disinfection regeneration anti obstacle to continuous cropping composition is made.
In specific implementation process, the glucides such as alcohol, glucose, sucrose, cellulose can also be used as liquid phase
Organic biomass.
Embodiment 4
1st, the soil disinfection obtained using embodiment 1 regenerates anti obstacle to continuous cropping composition product progress celery Pathogen of Fusarium Wilt
(Fusarium oxysporum)Go out effect test:
Celery droop is a kind of crushing soil-borne disease as caused by Fusarium oxysporum celery specialized form, seriously constrains celery
Production and peasant increase income.
1.1 test site basic conditions
Test arrangement is in Jiangsu Province Changzhou spring river modern agriculture(Vegetables)Industrial park, the field plant the greenhouses such as celery work throughout the year
Thing, soil degradation is serious, and in annual celery planting process, the celery droop incidence of disease is high, has formed obvious underproduction trend.
It is yellowish soil for examination soil, pH 5.1, the mS/cm of EC 0.468, total carbon 14.1g/kg, total nitrogen 2.4g/kg.
1.2 for studying thing
For studying thing:Celery, kind are imperial concubine's celery.
1.3 test process
Whole experimental plot is by fully smooth, it is ensured that fertility is uniform, then division of cells, plot area 200m2;Between each cell
If ridge building overlay film, individually the condition such as the row of filling, field fertilization, medication, irrigation is consistent.
Control group(CK):Soil tillage 20-30cm is poured water to maxmun field capacity, overlay film 18 days;
Experimental group(MAD):By Soil tillage 20-30cm, the soil disinfection regeneration anti obstacle to continuous cropping combination that embodiment 1 obtains is applied
Thing, sowing amount are 600kg/ mus, are well mixed with plowed soils;Then pour water to maxmun field capacity and overlay film, until soil
Earth oxidation-reduction potential is less than -60mV(18 days).
Processing takes off film soil sampling and carries out sterilization rate measure assessment after terminating.
The real-time fluorescence quantitative PCR measure of Fusarium oxysporum in 1.4 soil
The measure reference literature of Fusarium oxysporum:Huang X, Liu L, Wen T, Zhu R, Zhang J, Cai Z
(2015) Illumina MiSeq investigations on the changes of microbial community in
the Fusarium oxysporum f.sp. cubense infected soil during and after reductive
soil disinfestation. Microbiological Research 181:Method disclosed in 33-42. is carried out.
1.5 experimental result
The product of embodiment 1 is as shown in table 1 to the killing effect of celery Pathogen of Fusarium Wilt:
Killing effect of the present invention of table 1 to celery Pathogen of Fusarium Wilt
RSC continuous croppings agent is that the soil disinfection that the present embodiment obtains regenerates anti obstacle to continuous cropping composition in table 1.
2nd, using the product that embodiment 2 obtains to soil acidification and the improved test of elimination secondary salinization
2.1 test site basic conditions
Test arrangement is in Nanjing Jiangning District man of kylin door peasant household contracted field.The field plants the greenhouses such as cucumber work throughout the year
Thing, soil degradation is serious, and during annual green cucumber, the cucumber fusarium axysporum incidence of disease is high, has formed obvious underproduction trend.
It is yellowish soil for examination soil, pH 4.97, the mS/cm of EC 0.924, total carbon 12.1g/kg, total nitrogen 1.9g/kg.
2.2 for studying thing
For studying thing:Cucumber, kind are anti-No. 1 for south.
2.3 test process
Whole experimental plot is by fully smooth, it is ensured that fertility is uniform, then division of cells, plot area 200m2;Between each cell
If ridge building overlay film, individually the condition such as the row of filling, field fertilization, medication, irrigation is consistent.
Experiment sets two processing:
Control group(CK):Soil tillage 20-30cm is poured water to maxmun field capacity, overlay film 20 days;
Experimental group(MAD):By Soil tillage 20-30cm, the soil disinfection regeneration anti obstacle to continuous cropping combination that embodiment 2 obtains is applied
Thing, sowing amount are 500kg/ mus, are well mixed with plowed soils;Then pour water to maxmun field capacity and overlay film, until soil
Earth oxidation-reduction potential is less than -60mV(20 days).
Processing takes off film soil sampling and carries out soil acidity or alkalinity after 20 days(pH)And salinity(EC)Measure.
The measure of 2.4 soil pHs, EC
By soil and water with 1:2.5 ratio fully shakings mix, and use pH meter(Mettler S220K, Switzerland)Measure
Soil pH.By soil and the aqueous solution with 1:5(dw/w)Ratio mix, 200 r min-1Filtered after vibrating 1 h, use conductivity meter
Determine the EC of soil.
2.5 testing result
Control group and experimental group soil pH, EC testing results are as shown in Figure 1 and Figure 2.
From Fig. 1, Fig. 2, soil pH significantly improves after being improved through embodiment 2, and electrical conductivity significantly reduces, and shows this production
The effective improved soil acidifying of product and secondary salinization.
3rd, influence of the product obtained using embodiment 2 to the tomato incidence of disease and yield is tested
Bacterial wilt of tomato is a kind of soil-borne disease crushing as caused by Ralstonia solanacearum, and the occurrence degree of disease is with continuous cropping
The increase of the time limit and aggravate, directly result in the underproduction of tomato and the reduction of farmers' income
3.1 test site basic conditions
Test arrangement is in Nanjing Jiangning District man of kylin door peasant household contracted field.The field plants the greenhouses such as tomato work throughout the year
Thing, soil degradation is serious, and during annual tomato planting, the bacterial wilt of tomato incidence of disease is high, has formed obvious underproduction trend.
It is yellowish soil for examination soil, pH 4.25, the mS/cm of EC 0.734, total carbon 13.2g/kg, total nitrogen 2.2g/kg.
3.2 for studying thing
For studying thing:Tomato, kind are Soviet Union's powder 12.
3.3 test process
Whole experimental plot is by fully smooth, it is ensured that fertility is uniform, then division of cells, plot area 200m2;Between each cell
If ridge building overlay film, individually the condition such as the row of filling, field fertilization, medication, irrigation is consistent.
Experiment sets two processing:
Control group:Soil tillage 20-30cm is poured water to maxmun field capacity, overlay film 20 days;
Experimental group:By Soil tillage 20-30cm, the soil disinfection regeneration anti obstacle to continuous cropping composition that embodiment 3 obtains is applied, is applied
It is 700kg/ mus to enter amount, is well mixed with plowed soils;Then pour water to maxmun field capacity and overlay film, until Soil oxidation
Reduction potential is less than -60mV(20 days).
After processing 20 days, the statistics that film soil sampling carries out the incidence of disease and yield is taken off.
3.4 result of the test
The product of embodiment 2 is as shown in table 2 to the influence result of the test of the tomato incidence of disease and yield:
Influence of the present invention of table 2 to the tomato incidence of disease and yield
4th, the killing effect of Phytophthora capsici disease pathogen is tested using the product that embodiment 3 obtains
Phytophthora capsici disease is a kind of by the microbial crushing soil-borne disease of Phytophthora capsici, seriously constrains the production and agriculture of capsicum
The people's increases income.
4.1 test site basic conditions
Test arrangement is in Nanjing Jiangning District man of kylin door peasant household contracted field.The field plants the greenhouses such as capsicum work throughout the year
Thing, soil degradation is serious, and during annual tomato planting, the Phytophthora capsici disease incidence of disease is high, has formed obvious underproduction trend.
It is yellowish soil for examination soil, pH 4.63, the mS/cm of EC 0.711, total carbon 10.7g/kg, total nitrogen 2.1g/kg.
4.2 for studying thing
For studying thing:Capsicum, kind are Soviet Union green pepper No. 5.
4.3 test process
Whole experimental plot is by fully smooth, it is ensured that fertility is uniform, then division of cells, plot area 200m2;Between each cell
If ridge building overlay film, individually the condition such as the row of filling, field fertilization, medication, irrigation is consistent.
Experiment sets two processing:
Control group:Soil tillage 20-30cm is poured water to maxmun field capacity, and overlay film 19 days;
Experimental group:By Soil tillage 20-30cm, the soil disinfection regeneration anti obstacle to continuous cropping composition that embodiment 3 obtains is applied, is applied
It is 200kg/ mus to enter amount, is sprayed with equipment of sprinkler irrigation uniform;Then pour water to maxmun field capacity and overlay film, until Soil oxidation
Reduction potential is less than -60mV(19 days).
Processing takes off film soil sampling and carries out phytophthora blight of pepper quantity detection after 19 days.
The measure of phytophthora blight of pepper in 4.4 soil
The measure reference literature of Phytophthora capsici:Wen T, Huang X, Zhang J, Cai Z (2016) Effects of
biological soil disinfestation and water regime on suppressing Artemisia selengensis root rot pathogens. Journal of Soil Sediments 16:Disclosed in 215-225.
Method is carried out.
4.5 result of the test
Killing effect of 3 product of the present invention of table to Phytophthora capsici disease pathogen
In actual applications, solid phase organic biomass, which can use, includes cellulose, starch, wood wastes(As dry branches and fallen leaves,
Wood chip, pine bark), stalk, feces of livestock and poultry(Such as dried poultrymanure), insect excrement(Such as silkworm excrement), sugaring crop waste residue, plant and animal residues
One or more in fermentate, livestock manure fermented thing or insect manure fermentation thing, liquid phase organic biomass can use bag
The one or more in alcohol, glucose, sucrose, maltose or molasses are included, the purpose of invention can be achieved.
The explanation of above example is only intended to help the method and its core concept for understanding the present invention.It is described above to be only
The preferred embodiment of the present invention, it is noted that due to the finiteness of literal expression, and unlimited specific knot objectively be present
Structure, for those skilled in the art, under the premise without departing from the principles of the invention, it can also make some
Improve, retouch or change, above-mentioned technical characteristic can also be combined by rights;These improve retouching, change or
Combination, or the not improved guarantor that the design of invention and technical scheme are directly applied into other occasions, are regarded as the present invention
Protect scope.
Claims (10)
1. a kind of soil disinfection regenerates anti obstacle to continuous cropping composition, it is characterised in that said composition includes the following raw material:With quality
Percentages, special microorganism account for 0-5%, and organic catalysis thing accounts for 0-1%, and mineralizer accounts for 0-15%, and surfactant accounts for 0-1%, remaining
Measure as organic biomass.
2. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 1, it is characterised in that said composition is included such as
Lower raw material:By percentage to the quality, special microorganism accounts for 1-2%, and organic catalysis thing accounts for 0.1-0.4%, and mineralizer accounts for 1-2%, surface
Activating agent accounts for 0.1-0.3%, and surplus is organic biomass.
3. soil disinfection according to claim 1 or claim 2 regenerates anti obstacle to continuous cropping composition, it is characterised in that described special micro-
Biology is the one or more in saccharomycete, bacillus or fungi.
4. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 3, it is characterised in that the special microorganism
It is by saccharomycete, bacillus and fungi 3-4 in mass ratio:3-4:2-4 mixing obtains;The bacillus is solution starch bud
Spore bacillus.
5. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 3, it is characterised in that the organic catalysis thing
For cellulase.
6. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 3, it is characterised in that the organic biomass
Including solid phase organic biomass and liquid phase organic biomass;
Wherein, the solid phase organic biomass includes cellulose, starch, wood wastes and its fermentate, stalk and its fermentation
One or more in thing, feces of livestock and poultry and its fermentate, insect excrement and its fermentate, sugaring crop waste residue;
The liquid phase organic biomass includes the one or more in alcohol, glucose, sucrose, maltose, molasses.
7. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 3, it is characterised in that the mineralizer is made a living
Thing carbon or soil mineralization thing;
The soil mineralization thing is the one or more in glacial acetic acid, nitre or alunite.
8. soil disinfection regeneration anti obstacle to continuous cropping composition according to claim 3, it is characterised in that the surfactant
For nonionic/cloudy from bonding agent, nonionic penetrant, the one or more in cation permeability agent or anion-permeable membrane agent;
The anion-permeable membrane agent is one kind or more in stearic acid, quaternary ammonium compound, lecithin, alkyl glucoside or fatty glyceride
Kind.
9. the preparation method of soil disinfection regeneration anti obstacle to continuous cropping composition as claimed in claim 1, it is characterised in that specific step
It is rapid as follows:
Mixed after in proportion crushing special microorganism, organic catalysis thing, mineralizer, surfactant and solid phase organic biomass
Close, 70-100 DEG C of dry 10-60min, at room temperature natural cooling, that is, obtain the soil disinfection regeneration anti obstacle to continuous cropping combination
Thing;
Or in proportion mix special microorganism, organic catalysis thing, mineralizer, surfactant and liquid phase organic biomass,
Obtain the soil disinfection regeneration anti obstacle to continuous cropping composition.
10. application of the soil disinfection regeneration anti obstacle to continuous cropping composition as claimed in claim 1 in improved soil continuous cropping obstacle,
It is concretely comprised the following steps:
a)By soil rotary tillage 10-30cm, be manured into soil sterilizing regeneration anti obstacle to continuous cropping composition, 500-1000kg/ mus, and mixing is equal
It is even;
b)Soil is irrigated to maximum field capacity or overlay film, until soil redox potential is less than -60mV, that is, is completed to even
Make the improvement of soil.
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CN113201353A (en) * | 2021-05-17 | 2021-08-03 | 邯郸市明科生物技术有限公司 | Soil conditioner and preparation method thereof |
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