CN107325821A - A kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight and preparation method and application - Google Patents
A kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight and preparation method and application Download PDFInfo
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Abstract
The present invention discloses a kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight and preparation method and application, and the microbial inoculum includes following components：Biomass carbon, attapulgite, watermelon blight biocontrol bacteria, calcium superphosphate or nitro humus acid, sterilized water；The preparation method of the microbial inoculum is：By the absorption of biological and ecological methods to prevent plant disease, pests, and erosion bacterium solution on biomass carbon, add attapulgite and calcium superphosphate or nitro humus acid is mixed, appropriate amounts of sterilized water is added to reconcile, granulate, ventilation is dried under natural conditions, the microbial inoculum can be obtained after drying, the cfu/g of viable count in microbial inoculum >=5,000,000,000, particle diameter is 3 ~ 6 mm, pH value 6.5 ~ 7.5；Microbial inoculum of the present invention, which has, improves soil physico-chemical property, improves soil enzyme activities, promotes plant growth and improves the functions such as crop yield, and has preferable preventive and therapeutic effect to watermelon blight evil.
Description
Technical field
It is particularly a kind of suitable for a variety of the present invention relates to the microbial inoculum that the biomass carbon of agricultural technology field carries microorganism
Facility the preparation method of the solid microbial of crop and its prevent and treat watermelon blight evil in terms of application.
Background technology
China's facilities vegetable is developed rapidly in recent years, and cultivated area is up to 5832.6 ten thousand mu, under Protected Cultivation, and earth's surface is long
Phase culture underglass changes the original ecological environment of soil, and soil is often in high temperature, high humidity, high evaporation, the envelope without rain leaching
In closed loop metabolism environment, the physicochemical character and biological characteristics of soil change, soil hardening, porosity reduction, soil
Salinity table product phenomenon is obvious, the continuous worsening aggravation of soil quality.In addition agricultural chemicals, chemical fertilizer are excessively applied and deposited, heavy metal-polluted soil
Built up with the poisonous and harmful substance such as antibiotic, microbial population and structure change, the enhancing of geobiont toxicity, so that
The continuous cropping production obstacle for causing soil acidification, secondary salinization and soil-borne disease to aggravate is particularly thorny, as engagement device cultivation
Vegetables continue, the principal element of high-efficient development.
The agricultural measures of preventing and treating continuous cropping obstacle mainly have at present：（1）Implement crop rotation.Crop rotation and Intercrop and rotation are to solve continuous cropping
Obstacle the most simple and efficient method.Easily sent out by the crop rotation with pathogen non-host plant, or in high temperature continuous cropping diseases
Period mistake plantation, the cause of disease bacterium number in soil can be reduced significantly, but under many circumstances in order to improve economic effect
Benefit, has to carry out the continuous cropping of some vegetables, and the method often cannot get practical execution.（2）Soil disinfection.Soil disinfection
Including chemical agent sterilization and physical sterilization.Chemical agent method is main including the use of formalin, carbendazim or pentachloro- nitre
Base benzene is mixed soil or sterilized with Cosan, can also pour or mix the native ditch spread of medicine, processing soil of spreading manuer in holes with liquid medicine jet；Or use
Methyl bromide fumigation soil.Physical method mainly reduces the quantity of harmful bacteria using high temperature or low temperature.Summer high temperature season,
High temperature close plastic shed after pouring water, can also use to soil with high-temperature steam Pesticidal and sterilizing, can greatly reduce the quantity of soil-borne disease insect pest.
Low-temperature freezing, north of china in winter severe cold, it is impossible to the protecting field utilized, can remove and soil is ploughed deeply after film, freeze to death germ and worm's ovum.
Soil disinfection method is general also to cause crushing injury, particularly chemical disinfection while pest and disease damage is killed to some beneficial organisms
Method, expensive, the lasting period is short, it is also possible to destroy soil texture and environmental pollution, and prevention effect is undesirable, is unfavorable for big face
Product is promoted.（3）The application of resistant variety.It is to cause the key factor of continuous cropping obstacles that pest and disease damage, which is aggravated, and seed selection is resistant
New varieties and to be difficult to be bred as disease-resistant variety crop using Resistant rootstock carry out grafting cultivation, can effectively prevent a variety of
Soil-borne disease and the harm of nematode.But the problems such as being due to limitation and the seed selection cycle of disease-resistant variety resource, disease-resistant variety
Seed selection work much lags behind the speed of disease generation；While the method for grafting effectively solves soil-borne disease, crop is also brought
The problems such as product quality and mouthfeel decline.（4）The rational application of fertilizer.The special fertilizer applied at present in production is for mitigating continuous cropping barrier
Hinder effect substantially, in addition, applying organic manure is also the effective measures for mitigating continuous cropping obstacle.The generation of continuous cropping obstacle is related to work
Thing, soil, environment etc. are biological, abiotic many complicated factors, add influencing each other for these factors so that solve this
Problem is more complicated, any single measure or be all difficult to receive preferable effect by a few measure.Therefore, solve to set
The key for applying culturing vegetable production continuous cropping obstacle is to improve soil physical and chemical property, increases the quantity of soil beneficial microbe population,
Increase soil fertility.
The content of the invention
Good with fertilizer efficiency it is an object of the invention to provide not containing to environmentally hazardous substance, strong stress resistance changes with soil
Benign matter and the biomass carbon carrying microorganism for improving a kind of preventing and treating watermelon blights of resultant effect such as soil micro-ecosystem structure
Microbial inoculum, can effectively prevent and treat watermelon blight evil, the present invention is realized in：
A kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight evil, including solid phase components and liquid phase component；
Solid phase components：By percentage to the quality, biomass carbon 40-50%, attapulgite 20-25%, it is rotten with calcium superphosphate or nitro
Grow acid and supply surplus；
Liquid phase component：Including biocontrol bacteria zymotic fluid（Bacterial content is 1 ~ 9 × 1012 cfu/mL）And sterilized water；
Wherein, the volume mass ratio of biocontrol bacteria zymotic fluid and solid phase components（L/Kg）For 0.01-0.03:1;The quality of sterilized water
For the 30-50% of solid phase components quality, the cfu/g of the viable count of biocontrol microorganisms in microbial inoculum >=5,000,000,000.
Further, heretofore described biocontrol bacteria is the biocontrol bacteria of watermelon blight, is specifically as follows Te Jila buds
One or more in spore bacillus, Death Valley bacillus, dwell rice pseudomonad or bacillus subtilis.
Further, in the present invention, what the biomass carbon was obtained by：With agricultural wastes such as rice husk, crop materials
For raw material, with high temperature open tubular furnace isothermal holding 1-2 hours under 400-700 DEG C of anaerobic condition, room is naturally cooled to
Temperature, grinds and crosses 40 mesh sieves, and 121 DEG C of sterilizing 1h obtain biomass carbon.
Further, in the present invention, the attapulgite is that agricultural attapulgite is sustained granulating agent, preferably with mass percent
Meter, dioxide-containing silica 50.4%, aluminum oxide oxidation are 9.8%, iron oxide 4%, manganese oxide 0.04%, potassium oxide 1.0%, calcium oxide
1.8%, magnesia 0.04%, burn alkali 13.8%, and surplus is soil property composition；Calcium superphosphate be commercially available calcium superphosphate granular fertilizer, preferably its
In 80%~95% be dissolved in water, available phosphorus content >=12%.
The preparation method of the biomass carbon base microbe microbial inoculum of preventing and treating watermelon blight of the present invention, its specific steps is such as
Under：
Step one, it is 10 biocontrol bacteria fermentation to be made into bacterial content12Cfu/mL zymotic fluid, it is standby；
Step 2, biomass carbon and biocontrol bacteria zymotic fluid are mixed, biocontrol bacteria is fully adsorbed by biomass carbon, is mixed.
Step 3, by solid phase components（Biomass carbon, attapulgite, calcium superphosphate or/and nitro humus acid）Exist in proportion
It is well mixed under aseptic condition, adds sterilized water and be sufficiently stirred for, be then transferred into granulating disc and prepare granule, particle
A diameter of 3 ~ 6 mm, pH value 6.5 ~ 7.5 takes out particle in aeration-drying under natural conditions to pellet moisture levels≤20%（About 3-4
My god）, the cfu/g of the viable count of biocontrol microorganisms in microbial inoculum >=5,000,000,000 obtain the biomass carbon base microbe microbial inoculum.
Further, in the preparation method of the biomass carbon base microbe microbial inoculum of preventing and treating watermelon blight of the present invention, step
Biocontrol bacteria zymotic fluid refers in rapid one：Biocontrol bacteria is inoculated in LB fluid nutrient mediums, 37 ± 1 DEG C, 200rpm shaking table cultures
It is 10 to bacterial content12cfu/mL（About 48 hours）；
Application of the biomass carbon base microbe microbial inoculum in preventing and treating watermelon blight as described herein.
Further, application of the biomass carbon base microbe microbial inoculum in preventing and treating watermelon blight is invented to refer to：The bacterium
Agent is spreaded manuer in holes when 2 true leaves of watermelon seedling are transplanted, every plant of consumption 100g.
In the present invention, described LB fluid nutrient mediums are：Tryptone 10g, dusty yeast 5g, sodium chloride 10g, are added water to
1L, pH 7.0-7.5.
The characteristics of present invention utilizes the improved soil performance of charcoal and effectively adsorbs edaphon, it is abundant using its
The advantages of charcoal source, suitable pore structure and water conservation, plant endogenesis biocontrol bacteria is carried with biomass carbon, excellent set is made
Cultivating soil modifying agent is applied, improved soil performance can be reached, beneficial microbe purpose can be introduced in crop rhizosphere again.
Compared with prior art, the present invention is using rice husk, agricultural crop straw the like waste as raw material, and material is easy to get, preparation side
Method is simple, easy to use；The time-to-live of biocontrol microorganisms can be effectively improved；Increase the quantity of soil beneficial bacterium, strengthen soil enzyme
Vigor, increases soil fertility, and has stronger prevention and treatment to a variety of soil-borne diseases such as such as watermelon blight.
Brief description of the drawings
Fig. 1 prevents and treats the biomass carbon base microbe microbial inoculum particle picture of watermelon blight for the present invention；
Fig. 2 prevents and treats the biomass carbon base microbe microbial inoculum shelf life testing result schematic diagram of watermelon blight for the present invention；
Fig. 3 applies survival of the rear biocontrol microorganisms in soil for the biomass carbon base microbe microbial inoculum of present invention preventing and treating watermelon blight
Quantity schematic diagram；
Fig. 4 prevents and treats influence result signal of the biomass carbon base microbe microbial inoculum of watermelon blight to soil enzyme activities for the present invention
Figure；
Fig. 5 is for influence of the biomass carbon base microbe microbial inoculum to watermelon seedling growth of invention preventing and treating watermelon blight and to withered
The prevention effect schematic diagram of disease.
Embodiment
To facilitate a better understanding of the essence of inventive concept, the reality of the present invention is described in detail below by way of specific embodiment
Scheme is applied, is not as the restriction to the scope of the present invention.
Death Valley bacillus, Te Jila bacillus, hay bacillus are separated to the tomato plant of health in following embodiments,
By the purifying of Jiangsu Province Agriculture Science Institute home environment research department, identification, preserve.Withered germ of water-melon, which is separated to morbidity watermelon, plants
Strain, preservation is isolated and purified by home environment research department of Jiangsu agricultural science institution quality safety institute.
The raw material and culture medium being related in embodiment：
LB fluid nutrient mediums（1L）：Tryptone 10g, dusty yeast 5g, sodium chloride 10g, add water to 1L, pH 7.0-7.5；
LB solid mediums：Tryptone 10g, dusty yeast 5g, sodium chloride 10g, agar powder 20g, add water to 1L, pH 7.0-
7.5,121 DEG C of sterilizing 20min, are cooled down standby；
LB flat boards：LB solid mediums are melted in micro-wave oven, 50 DEG C or so is cooled to, sterilizing is poured into aseptic operating platform
In culture dish, LB flat boards are cooled down to obtain；
Attapulgite：Granulating agent is sustained purchased from the agricultural attapulgite of Changzhou Ding Bang mineral products Science and Technology Ltd.；Wherein dioxy
SiClx content 50.4%, aluminum oxide oxidation are 9.8%, iron oxide 4%, manganese oxide 0.04%, potassium oxide 1.0%, calcium oxide 1.8%, oxygen
Change magnesium 0.04%, burn alkali 13.8%, surplus is soil property composition；
Calcium superphosphate is commercially available calcium superphosphate granular fertilizer：Purchased from Sinofert Limited, wherein 80%~95% is dissolved in water, effectively
Phosphorus content >=12%.
Experiment equipment：
High temperature open tubular furnace is purchased from Shanghai Optics and Precision Mechanics institute, Chinese Academy of Sciences, model SG-GL1100K；
Embodiment 1 prepares the biomass carbon base microbe microbial inoculum of preventing and treating watermelon blight
Step one, biocontrol bacteria after purification is taken（Te Jila bacillus and Death Valley bacillus）, it is linked into LB Liquid Cultures
In base, 37 ± 1 DEG C of shaking tables（150 rpm）Culture 48 hours, it is 10 to obtain bacterial content12 Cfu/mL biocontrol bacteria zymotic fluid, it is standby
With.
Step 2, using rice husk as raw material, using 500 DEG C of high temperature open tubular furnace（In specific implementation process, tubular type furnace temperature
Degree can be controlled in the range of 400-700 DEG C）Biomass carbon is made under anaerobic condition, 40 mesh sieves were crushed, in 121 DEG C of autoclaves
Interior sterilizing 1h（In specific implementation process, can sterilize 1-2h according to actual conditions）After take out, obtain rice husk biomass carbon, it is standby.
Step 3, takes rice husk biomass carbon, attapulgite and calcium superphosphate that total amount is 20Kg（It is rice husk biomass carbon, recessed
Convex rod soil and calcium superphosphate are according to 4:2:1 mass ratio proportioning）, biological and ecological methods to prevent plant disease, pests, and erosion fermented liquid 0.2L in step one is taken, it is mixed with material charcoal
Close uniform, biomass carbon is fully adsorbed biocontrol microorganisms；Then remaining components are added, are well mixed, addition accounts for solid phase components total amount
After 50% sterilized water is stirred and evenly mixed, it is transferred in organic fertilizer granulating disc, 220v, 45 r/min, prepares particle within 2 hours
Agent, microbial-bacterial fertilizer granular size is 3 ~ 6 mm, then aeration-drying 3-4 days, humidity≤20% at 25 DEG C indoors.It can obtain
To biomass carbon base microbe microbial inoculum（As shown in Figure 1）, biocontrol microorganisms viable count is about 5,600,000,000 cfu/g in microbial inoculum.
In specific implementation process, Te Jila bacillus, Death Valley bacillus can be selected, dwell rice pseudomonad or withered
One or more preparation biological and ecological methods to prevent plant disease, pests, and erosion fermented liquids in careless bacillus, as long as ensuring that biological and ecological methods to prevent plant disease, pests, and erosion bacterial content is 10 in microbial inoculum12 cfu/
ML, can be achieved the purpose of invention；Sterilized water addition can be selected in the range of solid phase components quality 30-50%.
Used in the present embodiment is the biomass carbon prepared with rice husk, in concrete operations, other can also be used to plant
Biomass carbon prepared by thing stalk.
The shelf life experiment of the microbial inoculum of embodiment 2
(1) the biocontrol bacteria zymotic fluid for obtaining the step one of embodiment 1 is diluted with physiological saline makes it be obtained with step 3
The initial of biomass carbon base microbe microbial inoculum measure identical, bacteria containing amount is about 5,600,000,000 cfu/g, and Room-temperature seal is preserved.
(2) biocontrol microorganisms quantitative measurement in biocontrol bacteria zymotic fluid and biomass carbon base microbe microbial inoculum：Take 50 mL biological and ecological methods to prevent plant disease, pests, and erosions thin
Fermented liquid（Or 50g biomass carbon base microbe microbial inoculums）, add sterilized water and be settled to 1000 mL, be put into shaking table 30 DEG C 200
R/min shakes 30min；10 mL solution are taken into 100 mL volumetric flasks, constant volume to 100 mL, then 10 times dilution 5 times after, take 100
UL coated plates on LB flat boards, 30 DEG C are inverted 12 h of culture, are counted clump count, are set 3 repetitions；Every milliliter of zymotic fluid（Every gram of bacterium
Agent）In viable count=(a*n*v1)/(v2* m), a in formula:Plate count average value, n:Extension rate, v1：Product are initial
Dilute volume, v2：Apply the dilution bacterium solution volume of flat board, m：Sample volume.
(3) shelf life detection sample time continues 12 months.
(4) experimental result is as shown in Fig. 2 from Figure 2 it can be seen that zymotic fluid when detecting for 7th month, viable count is 1.0 × 107
Cfu/mL, and biomass carbon base microbe microbial inoculum 12nd month detect when, viable count be 1.4 × 108Cfu/g, utilizes stalk
Biomass carbon carries microorganism and is made after biomass carbon base microbe microbial inoculum, significantly extends shelf life.
Influence after the administration of the biomass carbon base microbe microbial inoculum of embodiment 3 to soil enzyme activities
(1) in order to exclude the interference of miscellaneous bacteria in soil, the bacillus in biomass carbon base microbe microbial inoculum is resisted first
Raw element mark：Bacillus is inoculated on the LB flat boards containing 1 μ g/mL rifampins, the concentration of rifampin is stepped up to 300 μ
G/mL, growth and the physiological property strains consistent with original strain can be stablized by filtering out, according to embodiment 1
Step prepares biomass carbon base microbe microbial inoculum；
(2) 2 processing are set：
A. the kg of land 10 is taken, step is added according to the ratio of mass percent 2%（1）The biomass carbon base microbe bacterium of acquisition
Room-temperature seal preservation is placed into after agent, mixing；
B. resistant strain prepares biocontrol bacteria zymotic fluid according to step one method in embodiment 1, is connect according to the total amount of bacterium in processing A
Enter in 10 kg field soils, mix Room-temperature seal and preserve；
(3) amount of survival of the biocontrol microorganisms in soil is determined：Step in assay method be the same as Example 2（2）；
(4) soil enzyme activities is determined：After above-mentioned processing 30 days, each processing takes 10 g soil samples, determine soil cellulase, in
Property protease, invertase, urase, catalase, dehydrogenase activity enzymatic activity, experimental procedure referring to Suzhou section engrave biotechnology
Co., Ltd's soil enzymatic determination kit specification（http://www.cominbio.com/a/chanpinzhanshi/
ziyoupinpaishijihe/turangxilie/）.
(5) amount of survival result of the biocontrol microorganisms in soil is as shown in figure 3, show inoculation biocontrol microorganisms fermentation liquor treatment 7 days
When detection viable count be 6.72 × 109Cfu/g, but with the extension of time, viable count declines rapidly, to viable bacteria at 120 days
Number is only 2.5 × 108cfu/g；And the living bacteria count of biomass carbon base microbe microbial inoculum processing is then one by slowly discharging
Increased rapidly to quantity in the process slowly declined, when handling 7 days, detection viable count only has 7.03 × 109Cfu/g, still
Viable count reaches peak value 9.83 × 10 when handling 21 days9Cfu/g, when viable count is slowly declined to handle 120 days afterwards, effectively
Viable count still has 2.98 × 109Cfu/g, illustrates significantly to extend using the microbial inoculum that biomass carbon carrying bacillus is made
Time-to-live of the biocontrol microorganisms in soil.
(6) soil enzyme activities result is as shown in figure 4, in Fig. 4, NS is land；B is bacterium solution (processing B)；BC is microbial inoculum
（Handle A）；
From fig. 4, it can be seen that significantly enhancing soil cellulase, sugarcane after being handled using biomass carbon base microbe microbial inoculum of the present invention
Carbohydrase, urase, catalase, the activity of dehydrogenase activity enzyme, but neutral proteinase activity reduction, this may be unfavorable for soil
High molecular weight protein is degraded to the products such as amino acid in earth.
The watermelon seedling growth of embodiment 4 and droop prevention effect pot experiment
Pot experiment is carried out in the heliogreenhouse of Jiangsu Province Agriculture Science Institute.If 4 processing：
A. sterilized soil+inoculation withered germ of water-melon；
B. sterilized soil+inoculation withered germ of water-melon+biomass carbon base microbe microbial inoculum auxiliary material（It is sterile, specially in embodiment 1
Prepare the auxiliary material of biomass carbon base microbe microbial inoculum, i.e., without biocontrol microorganisms composition, the life that other compositions content be the same as Example 1 is obtained
Material charcoal base microbe microbial inoculum, addition is the 0.5% of sterilized soil quality）；
C. the biomass carbon base microbe microbial inoculum of sterilized soil+withered germ of water-melon+0.5%（The biomass carbon prepared in embodiment 1
Base microbe microbial inoculum, addition is the 0.5% of sterilized soil quality）；
D. sterilized soil+biomass carbon base microbe microbial inoculum of withered germ of water-melon+2%（For the biomass carbon base prepared in embodiment 1
Microbial bacterial agent, addition is the 2% of sterilized soil quality）.
For examination variety of watermelon No. 1 is created for Soviet Union.
Tests concrete steps are as follows：
(1) by after watermelon seed surface sterilization, sterilized water soaked overnight, 30 DEG C of constant temperature 2d vernalization.Hole tray is seeded in after showing money or valuables one carries unintentionally,
When seedling length to 2 true leaves, transplant to the cm of internal diameter 10, high 15 cm, in the plastic cup of built-in sterile soil.
(2) four treatment groups add soil for 300 g sterilized soils, are separately added into C and D treatment group sterilized soils（Account for and go out
Bacterium soil mass percent）The biomass carbon base microbe microbial inoculum that 0.5% and 2% step 3 of embodiment 1 is obtained.
(3) watermelon seedlings（2 leaf periods）While transplanting, root fills 20 ml 1 in treatment group A, B, C and D treatment group
×107Cfu/mL withered germ of water-melon spore suspensions, are inoculated with withered germ of water-melon.
(4) after inoculation disease fungi, plant incidence is investigated, treats only to connect disease fungus group（A processing）60% seedling goes out
After existing disease symptom, terminate experiment, statistics watermelon seedling growing state, disease index and the incidence of disease.
(5) the prevention effect experimental result of watermelon seedling stage droop is as shown in table 1 and Fig. 5：
Prevention effect of the biomass carbon base microbe microbial inoculum of table 1 to watermelon seedling stage droop
By table 1 is visible and Fig. 5（A-D corresponds to above-mentioned processing A-D respectively in Fig. 5）It can be seen that, access embodiment obtains microbial inoculum group（C、D
Processing））The generation of watermelon blight can be effectively prevented after inoculation watermelon plant, the incidence of disease is remarkably decreased 70- compared with A processing
85% or so, highest preventive effect is up to 74.6%.
(6) watermelon seedling growth influence result is as shown in table 2：
Influence of the biomass carbon base microbe microbial inoculum of table 2 to watermelon seedling growth
Note：A:Connect germ；B:Connect germ+microbial inoculum auxiliary material；C：Connect the microbial inoculum of germ+0.5%；D：Connect the microbial inoculum of germ+2%.Small letter in table
Letter represents 0.05 level difference conspicuousness
Result of the test shows that the microbial inoculum can significantly inhibit injury of the withered germ of water-melon to watermelon seedling growth, and can promote
Enter the growth in watermelon seedling stage, added in soil after the 2% solid bacterial manure, remarkably promote watermelon seedling stage plant height, root length and increase about
75-90 or so, promotes the growth by 55% or so of the biomass such as fresh weight and dry weight.
Claims (8)
1. a kind of biomass carbon base microbe microbial inoculum for preventing and treating watermelon blight, including following solid phase components and liquid phase component；
Solid phase components：By percentage to the quality, biomass carbon 40-50%, attapulgite 20-25%, it is rotten with calcium superphosphate or nitro
Grow acid and supply surplus；
Liquid phase component：Including biocontrol bacteria zymotic fluid and sterilized water；
Wherein, the volume mass ratio of biocontrol bacteria zymotic fluid and solid phase components is 0.01-0.03:1；The quality of sterilized water is solid phase
The cfu/g of the viable count of biocontrol bacteria in the 30-50% of constituent mass, microbial inoculum >=5,000,000,000.
2. according to claim 1 it is a kind of prevent and treat watermelon blight biomass carbon base microbe microbial inoculum, it is characterised in that institute
State biocontrol bacteria for Te Jila bacillus, Death Valley bacillus, one kind in dwell rice pseudomonad or bacillus subtilis or
It is a variety of.
3. according to claim 1 it is a kind of prevent and treat watermelon blight biomass carbon base microbe microbial inoculum, it is characterised in that institute
State what biomass carbon was obtained by：Using agricultural wastes as raw material, it is made, is ground after cooling under 400-700 DEG C of anaerobic condition
And 40 mesh sieves are crossed, 121 DEG C of sterilizing 1-2h produce the biomass carbon.
4. according to claim 3 it is a kind of prevent and treat watermelon blight biomass carbon base microbe microbial inoculum, it is characterised in that institute
Agricultural wastes are stated for one or both of rice husk or stalk.
5. the preparation method of the biomass carbon base microbe microbial inoculum of watermelon blight is prevented and treated as described in claim 1-4 is any, its
It is characterised by, comprises the following steps that：
Step one, it is 10 biocontrol bacteria fermentation to be made into bacterial content12 Cfu/mL biocontrol bacteria zymotic fluids, it is standby；
Step 2, biocontrol bacteria zymotic fluid and biomass carbon are mixed, remaining components are then added, is well mixed, granulation, particle
Then a diameter of 3 ~ 6 mm, pH value 6.5 ~ 7.5 is dried to pellet moisture levels≤20%, that is, obtains the life of the preventing and treating watermelon blight
Material charcoal base microbe microbial inoculum.
6. the preparation method of the biomass carbon base microbe microbial inoculum of watermelon blight, its feature are prevented and treated according to claim 5
It is, biocontrol bacteria zymotic fluid refers to described in step one：Biocontrol bacteria is inoculated in LB liquid medium, 37 ± 1 DEG C,
It is 10 to be cultivated in 200rpm shaking tables to bacterial content12cfu/mL。
7. the biomass carbon base microbe microbial inoculum that watermelon blight is prevented and treated as described in claim 1-4 is any is withered in preventing and treating watermelon
Application in disease.
8. application as claimed in claim 7, it is characterised in that by the biomass carbon base microbe of the preventing and treating watermelon blight
Microbial inoculum is spreaded manuer in holes when watermelon seedlings are transplanted into every plant of consumption 100g, to prevent and treat watermelon blight.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107814636A (en) * | 2017-12-07 | 2018-03-20 | 江苏省农业科学院 | A kind of charcoal base biological organic fertilizer and preparation method and application |
| CN108782134A (en) * | 2018-06-27 | 2018-11-13 | 淮南市永光草莓专业合作社 | A kind of western muskmelon tailored version seedling medium and preparation method thereof |
| CN111269067A (en) * | 2019-12-20 | 2020-06-12 | 中国农业科学院西部农业研究中心 | Saline-alkali soil improver and application thereof in improvement of watermelon saline-alkali soil |
| CN111320985A (en) * | 2020-01-19 | 2020-06-23 | 河南省中威春雨植物营养有限公司 | Biochar compound and application thereof in soil improvement |
| CN112317528A (en) * | 2020-10-29 | 2021-02-05 | 盛世生态环境股份有限公司 | Method for combined remediation of heavy metal contaminated soil |
| CN115594533A (en) * | 2022-10-28 | 2023-01-13 | 滨州学院（Cn） | Additive for improving decomposition degree of cow dung compost and application thereof |
| CN117025437A (en) * | 2023-03-03 | 2023-11-10 | 淮阴师范学院 | Biochar-based microbial agent for preventing and treating soil-borne diseases of solanaceous vegetables and preparation method thereof |
| CN118184449A (en) * | 2024-04-16 | 2024-06-14 | 中国热带农业科学院热带作物品种资源研究所 | Organic compound fertilizer for preventing banana wilt |
| CN118184449B (en) * | 2024-04-16 | 2024-10-25 | 中国热带农业科学院热带作物品种资源研究所 | Organic compound fertilizer for preventing banana wilt |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107814636A (en) * | 2017-12-07 | 2018-03-20 | 江苏省农业科学院 | A kind of charcoal base biological organic fertilizer and preparation method and application |
| CN108782134A (en) * | 2018-06-27 | 2018-11-13 | 淮南市永光草莓专业合作社 | A kind of western muskmelon tailored version seedling medium and preparation method thereof |
| CN111269067A (en) * | 2019-12-20 | 2020-06-12 | 中国农业科学院西部农业研究中心 | Saline-alkali soil improver and application thereof in improvement of watermelon saline-alkali soil |
| CN111269067B (en) * | 2019-12-20 | 2022-03-15 | 中国农业科学院西部农业研究中心 | Saline-alkali soil improver and application thereof in improvement of watermelon saline-alkali soil |
| CN111320985A (en) * | 2020-01-19 | 2020-06-23 | 河南省中威春雨植物营养有限公司 | Biochar compound and application thereof in soil improvement |
| CN112317528A (en) * | 2020-10-29 | 2021-02-05 | 盛世生态环境股份有限公司 | Method for combined remediation of heavy metal contaminated soil |
| CN115594533A (en) * | 2022-10-28 | 2023-01-13 | 滨州学院（Cn） | Additive for improving decomposition degree of cow dung compost and application thereof |
| CN117025437A (en) * | 2023-03-03 | 2023-11-10 | 淮阴师范学院 | Biochar-based microbial agent for preventing and treating soil-borne diseases of solanaceous vegetables and preparation method thereof |
| CN118184449A (en) * | 2024-04-16 | 2024-06-14 | 中国热带农业科学院热带作物品种资源研究所 | Organic compound fertilizer for preventing banana wilt |
| CN118184449B (en) * | 2024-04-16 | 2024-10-25 | 中国热带农业科学院热带作物品种资源研究所 | Organic compound fertilizer for preventing banana wilt |
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