CN108782134A - A kind of western muskmelon tailored version seedling medium and preparation method thereof - Google Patents

A kind of western muskmelon tailored version seedling medium and preparation method thereof Download PDF

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Publication number
CN108782134A
CN108782134A CN201810681666.6A CN201810681666A CN108782134A CN 108782134 A CN108782134 A CN 108782134A CN 201810681666 A CN201810681666 A CN 201810681666A CN 108782134 A CN108782134 A CN 108782134A
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China
Prior art keywords
parts
hull ash
rice hull
seedling medium
button mushroom
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CN201810681666.6A
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Chinese (zh)
Inventor
王朋成
杨积冠
严从生
俞家永
俞飞飞
杨积山
贾利
江海坤
董言香
田红梅
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Huainan Wiko Strawberry Professional Cooperative
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Huainan Wiko Strawberry Professional Cooperative
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Priority to CN201810681666.6A priority Critical patent/CN108782134A/en
Publication of CN108782134A publication Critical patent/CN108782134A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of western muskmelon tailored version seedling mediums, are composed of the following raw materials in parts by weight:40-50 parts of rice hull ash, 15-20 parts of button mushroom dregs, 10-15 parts of vermiculite, 0.5-1 parts of ammonium phosphate, 0.5-1 parts of calcium superphosphate, 0.05-0.1 parts of palmitic acid;The invention also discloses the preparation methods of the seedling medium, include the following steps:(1) rice hull ash is pre-processed;(2) button mushroom dregs are prepared;(3) palm acid solution is prepared;(4) host matrix is prepared;(5) bacillus subtilis strain is cultivated;(6) by matrix inoculation fermentation liquid.Seedling medium prepared by the present invention can promote the growth of rice shoot, and play strong sprout, keep greener blade, leaf area increase, root system more flourishing, its droop incidence reduces after field planting, and yield increases.

Description

A kind of western muskmelon tailored version seedling medium and preparation method thereof
Technical field
The invention belongs to western muskmelon seedling-raising technique fields, and in particular, to a kind of western muskmelon tailored version seedling medium and its Preparation method.
Background technology
Watermelon is Curcurbitaceae, the annual herbaceous plant of Citrullus.Watermelon contains abundant nutriment, water content Height, natural nutrition juice content ranks first in all fruit, is referred to as " king of summer melon and fruit ", it has become people and likes Good merchantable brand of relieving summer heat, throughout the country extensively cultivation.Since growth of watermelon area expands year by year, the problems such as continuous cropping obstacle also therewith Come, wherein droop can the underproduction 15%, severe one is up to 85%, or even total crop failure, watermelon blight be endangered in watermelon disease it is the tightest Weight, causes one of maximum disease of economic loss.People usually solve the problems, such as this with chemistry of pesticide medicament, however give environment Negative effect is brought with human health.
In melon cultivation, melon crop nursery is to shorten watermelon in field remaining time, rationally adjusts crops for rotation, saves soil Ground, labour, time realize the requisite measure of kipeming high yield.The melon special seedling substrate that melon tradition nursery is selected also has Many advantages, such as planting percent height, abridged edition section kind, easy to operation and transport etc., but the nutrient supply problem in melon nursery, apprentice The problem of long problem and the melon seedling field anti-blight difference routinely brought out still cannot disposably be solved by matrix;It is existing Melon seedling medium nursery, it is easy to which the excessive growth for causing rice shoot is not easy to cultivate strong sprout, if rice shoot does not take other arrange after field planting It applies and easily feels droop, seriously affected the yield of watermelon, reduce the planting benefit of watermelon, also easily cause pollution.
Invention content
The purpose of the present invention is to provide a kind of western muskmelon tailored version seedling mediums and preparation method thereof, to solve existing skill Following technical problem in art:(1) existing melon seedling medium nursery is not easy to cultivate strong sprout;(2) the anti-of rice shoot cannot be improved Droop ability.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of western muskmelon tailored version seedling medium, is composed of the following raw materials in parts by weight:40-50 parts of rice hull ash, White mushroom 15-20 parts of bacteria residue, 10-15 parts of vermiculite, 0.5-1 parts of ammonium phosphate, 0.5-1 parts of calcium superphosphate, 0.05-0.1 parts of palmitic acid;
The seedling medium is prepared by following steps:
(1) rice hull ash is first subjected to desalting processing, salinity is adjusted to 1.4-1.5mS/cm, sulfuric acid-ferrous sulfate is added Mixed liquor adjusts the pH value of rice husk buck to 6.6-7.0, ammonium phosphate is added, is uniformly mixed, 5mm sieves are crossed after dry, are made Pre-process rice hull ash;
(2) button mushroom is cleaned, is put into -15 DEG C to -10 DEG C of environment and freezes 7-11d, after the completion, taken out and shine dry doubling 5mm sieves are smashed it through, button mushroom dregs are made;
(3) use acetone as solvent, compound concentration is the palm acid solution of 0.5mmol/L, spare;
(4) pretreatment rice hull ash, button mushroom dregs, vermiculite and calcium superphosphate are uniformly mixed, add palm acid solution, It is uniformly mixed, host matrix;
(5) it will be stored in the bacillus subtilis strain in -18 DEG C of refrigerators, is activated in beef-protein medium, Constant temperature incubation 15-18h is inoculated into Landy culture mediums by 2% inoculum concentration and expands numerous, and isothermal vibration culture 48-60h must ferment Liquid, it is spare;
(6) by host matrix in high-pressure sterilizing pot high temperature sterilizing 1.5-2h, after cooling, inoculation fermentation liquid, connects in proportion Matrix after bacterium is placed in constant temperature and humidity culture 3-5d in 30 DEG C of incubators, the moisture that room temperature is dried into mixture that is placed on contain Amount is less than 30%, is made.
A kind of preparation method of western muskmelon tailored version seedling medium, includes the following steps:
(1) rice hull ash is first subjected to desalting processing, salinity is adjusted to 1.4-1.5mS/cm, sulfuric acid-ferrous sulfate is added Mixed liquor adjusts the pH value of rice husk buck to 6.6-7.0, ammonium phosphate is added, is uniformly mixed, 5mm sieves are crossed after dry, are made Pre-process rice hull ash;
(2) button mushroom is cleaned, is put into -15 DEG C to -10 DEG C of environment and freezes 7-11d, after the completion, taken out and shine dry doubling 5mm sieves are smashed it through, button mushroom dregs are made;
(3) use acetone as solvent, compound concentration is the palm acid solution of 0.5mmol/L, spare;
(4) pretreatment rice hull ash, button mushroom dregs, vermiculite and calcium superphosphate are uniformly mixed, add palm acid solution, It is uniformly mixed, host matrix;
(5) it will be stored in the bacillus subtilis strain in -18 DEG C of refrigerators, is activated in beef-protein medium, Constant temperature incubation 15-18h is inoculated into Landy culture mediums by 2% inoculum concentration and expands numerous, and isothermal vibration culture 48-60h must ferment Liquid, it is spare;
(6) by host matrix in high-pressure sterilizing pot high temperature sterilizing 1.5-2h, after cooling, inoculation fermentation liquid, connects in proportion Matrix after bacterium is placed in constant temperature and humidity culture 3-5d in 30 DEG C of incubators, the moisture that room temperature is dried into mixture that is placed on contain Amount is less than 30%, is made.
Further, it is constant temperature under the conditions of 36-38 DEG C, 150r/min that constant temperature incubation condition is activated described in step (5) Cultivate 15-18h.
Further, it is isothermal vibration under the conditions of 29-31 DEG C, 160r/min to expand numerous condition of culture described in step (5) Cultivate 48-60h.
Further, inoculative proportion described in step (6) is 95-102mL/kg.
Beneficial effects of the present invention:
The functional form seedling medium that the present invention makes is to be aided with palm using rice hull ash, button mushroom dregs, vermiculite as raw material Sour component, and it is inoculated with bacillus subtilis, palmitic acid has the advantages that environmentally friendly, pollution-free, resistance pathogen, can stablize and colonize To inside the root system of cotton seedling, the combined use of palmitic acid and strain can inhibit infecting and breeding, western sweet tea for wilt After melon seedling replanting, the generation of droop can be substantially reduced;Vermiculite can enable that matrix is not hardened after nursery while having water conservation Power;Rice hull ash and button mushroom dregs have fertilizer efficiency, can play strong sprout effect;Seedling medium can promote the growth of rice shoot, and rise Strong sprout acts on, and keeps greener blade, leaf area increase, root system more flourishing, its droop incidence reduces after field planting, and yield increases.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with embodiment, it is clear that described reality It is only a part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, this field is general All other embodiment that logical technical staff is obtained without creative efforts belongs to what the present invention protected Range.
A kind of western muskmelon tailored version seedling medium, is composed of the following raw materials in parts by weight:40-50 parts of rice hull ash, White mushroom 15-20 parts of bacteria residue, 10-15 parts of vermiculite, 0.5-1 parts of ammonium phosphate, 0.5-1 parts of calcium superphosphate, 0.05-0.1 parts of palmitic acid;
The preparation method of the seedling medium, includes the following steps:
(1) rice hull ash is first subjected to desalting processing, salinity is adjusted to 1.4-1.5mS/cm, sulfuric acid-ferrous sulfate is added Mixed liquor adjusts the pH value of rice husk buck to 6.6-7.0, ammonium phosphate is added, is uniformly mixed, 5mm sieves are crossed after dry, are made Pre-process rice hull ash;
Pretreated rice hull ash contains abundant rice husk mineral element, and the rice hull ash by desalting processing can melt with ammonium phosphate It closes, nitrogen source is provided for matrix;
(2) button mushroom is cleaned, is put into -15 DEG C to -10 DEG C of environment and freezes 7-11d, (wherein naked eyes are killed in freezing Visible fruit body of edible fungi), after the completion, takes out solarization dry doubling and smash it through 5mm sieves, button mushroom dregs are made;
(3) use acetone as solvent, compound concentration is the palm acid solution of 0.5mmol/L, spare;
Palmitic acid has the advantages that environmentally friendly, pollution-free, resistance pathogen;
(4) pretreatment rice hull ash, button mushroom dregs, vermiculite and calcium superphosphate are uniformly mixed, add palm acid solution, It is uniformly mixed, host matrix;
(5) it will be stored in the bacillus subtilis strain in -18 DEG C of refrigerators, is activated in beef-protein medium, Isothermal vibration culture 15-18h, is inoculated into Landy culture mediums by 2% inoculum concentration and is expanded under the conditions of 36-38 DEG C, 150r/min Numerous, isothermal vibration culture 48-60h, obtains zymotic fluid under the conditions of 29-31 DEG C, 160r/min, spare;
(6) by host matrix in high-pressure sterilizing pot high temperature sterilizing 1.5-2h, after cooling, in proportion (95-102mL/kg) Inoculation fermentation liquid connects the matrix after bacterium and is placed in constant temperature and humidity culture 3-5d in 30 DEG C of incubators, the room temperature that is placed on dry to mixed The moisture closed in object is less than 30%, is made.
By inoculation bacillus subtilis, which can stablize colonizes inside the root system of cotton seedling seedling medium, suppression Wilt processed is infected and is bred, and after seedling replanting, the generation of droop can be substantially reduced.
Embodiment 1
A kind of western muskmelon tailored version seedling medium, is composed of the following raw materials in parts by weight:40 parts of rice hull ash, button mushroom dregs 15 parts, 10 parts of vermiculite, 0.5 part of ammonium phosphate, 0.5 part of calcium superphosphate, 0.05 part of palmitic acid;
Embodiment 2
A kind of western muskmelon tailored version seedling medium, is composed of the following raw materials in parts by weight:40-50 parts of rice hull ash, White mushroom 18 parts of bacteria residue, 12 parts of vermiculite, 0.8 part of ammonium phosphate, 0.7 part of calcium superphosphate, 0.07 part of palmitic acid;
Embodiment 3
A kind of western muskmelon tailored version seedling medium, is composed of the following raw materials in parts by weight:50 parts of rice hull ash, button mushroom dregs 20 parts, 15 parts of vermiculite, 1 part of ammonium phosphate, 1 part of calcium superphosphate, 0.1 part of palmitic acid.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification, It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably explain the present invention Principle and practical application, to enable skilled artisan to be best understood by and utilize the present invention.The present invention is only It is limited by claims and its full scope and equivalent.

Claims (5)

1. a kind of western muskmelon tailored version seedling medium, which is characterized in that be composed of the following raw materials in parts by weight:Rice hull ash 40-50 Part, 15-20 parts of button mushroom dregs, 10-15 parts of vermiculite, 0.5-1 parts of ammonium phosphate, 0.5-1 parts of calcium superphosphate, palmitic acid 0.05-0.1 Part;
The seedling medium is prepared by following steps:
(1) rice hull ash is first subjected to desalting processing, salinity is adjusted to 1.4-1.5mS/cm, sulfuric acid-ferrous sulfate mixing is added Liquid adjusts the pH value of rice husk buck to 6.6-7.0, ammonium phosphate is added, is uniformly mixed, 5mm sieves are crossed after dry, pre- place is made Manage rice hull ash;
(2) button mushroom is cleaned, is put into -15 DEG C to -10 DEG C of environment and freezes 7-11d, after the completion, taken out and shine dry doubling crushing 5mm sieves are crossed afterwards, and button mushroom dregs are made;
(3) use acetone as solvent, compound concentration is the palm acid solution of 0.5mmol/L, spare;
(4) pretreatment rice hull ash, button mushroom dregs, vermiculite and calcium superphosphate are uniformly mixed, adds palm acid solution, stirred It is uniformly mixed, host matrix;
(5) it will be stored in the bacillus subtilis strain in -18 DEG C of refrigerators, is activated in beef-protein medium, constant temperature 15-18h is cultivated, is inoculated into Landy culture mediums by 2% inoculum concentration and expands numerous, isothermal vibration culture 48-60h obtains zymotic fluid, It is spare;
(6) host matrix is sterilized 1.5-2h in high-pressure sterilizing pot high temperature, after cooling, inoculation fermentation liquid in proportion, after connecing bacterium Matrix be placed in constant temperature and humidity culture 3-5d in 30 DEG C of incubators, to be placed on the moisture that room temperature is dried into mixture small In 30%, it is made.
2. a kind of preparation method of western muskmelon tailored version seedling medium according to claim 1, which is characterized in that including such as Lower step:
(1) rice hull ash is first subjected to desalting processing, salinity is adjusted to 1.4-1.5mS/cm, sulfuric acid-ferrous sulfate mixing is added Liquid adjusts the pH value of rice husk buck to 6.6-7.0, ammonium phosphate is added, is uniformly mixed, 5mm sieves are crossed after dry, pre- place is made Manage rice hull ash;
(2) button mushroom is cleaned, is put into -15 DEG C to -10 DEG C of environment and freezes 7-11d, after the completion, taken out and shine dry doubling crushing 5mm sieves are crossed afterwards, and button mushroom dregs are made;
(3) use acetone as solvent, compound concentration is the palm acid solution of 0.5mmol/L, spare;
(4) pretreatment rice hull ash, button mushroom dregs, vermiculite and calcium superphosphate are uniformly mixed, adds palm acid solution, stirred It is uniformly mixed, host matrix;
(5) it will be stored in the bacillus subtilis strain in -18 DEG C of refrigerators, is activated in beef-protein medium, constant temperature 15-18h is cultivated, is inoculated into Landy culture mediums by 2% inoculum concentration and expands numerous, isothermal vibration culture 48-60h obtains zymotic fluid, It is spare;
(6) host matrix is sterilized 1.5-2h in high-pressure sterilizing pot high temperature, after cooling, inoculation fermentation liquid in proportion, after connecing bacterium Matrix be placed in constant temperature and humidity culture 3-5d in 30 DEG C of incubators, to be placed on the moisture that room temperature is dried into mixture small In 30%, it is made.
3. a kind of preparation method of western muskmelon tailored version seedling medium according to claim 2, which is characterized in that step (5) it is constant temperature incubation 15-18h under the conditions of 36-38 DEG C, 150r/min that constant temperature incubation condition is activated described in.
4. a kind of preparation method of western muskmelon tailored version seedling medium according to claim 2, which is characterized in that step (5) it is isothermal vibration culture 48-60h under the conditions of 29-31 DEG C, 160r/min to expand numerous condition of culture described in.
5. a kind of preparation method of western muskmelon tailored version seedling medium according to claim 2, which is characterized in that step (6) inoculative proportion described in is 95-102mL/kg.
CN201810681666.6A 2018-06-27 2018-06-27 A kind of western muskmelon tailored version seedling medium and preparation method thereof Pending CN108782134A (en)

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