CN110178854A - For restoring composition and its application of soil disinfection by force - Google Patents
For restoring composition and its application of soil disinfection by force Download PDFInfo
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- CN110178854A CN110178854A CN201910511457.1A CN201910511457A CN110178854A CN 110178854 A CN110178854 A CN 110178854A CN 201910511457 A CN201910511457 A CN 201910511457A CN 110178854 A CN110178854 A CN 110178854A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The present invention discloses composition and its application for restoring soil disinfection by force.The composition includes the cellulose degradation composite bacteria agent being made of Yue Shi Flavobacterium and Pseudomonas fluorescens, further also may include corn stover.Composition of the invention can be changed structure of soil microbial community, promote Corn Stalk Decomposition to generate the substance that ammonia, methane, organic acid and hydrogen sulfide etc. have toxic action to Fusarium oxysporum in an anaerobic environment.Composition through the invention can kill 99.9% Fusarium oxysporum, 97.0% pathogen is still persistently killed between falling dry spell, while this group is combined can significantly improve soil physico-chemical property, and soil secondary salinization is effectively relieved, and then can be used for the prevention and treatment of tomato wilt, have the effect of improving.
Description
Technical field
The present invention relates to strong reduction soil disinfection fields, more particularly to the composition for restoring soil disinfection by force, suppression
Method, the method for the strong reduction soil disinfection of improvement and the answering in prevention and treatment tomato wilt of metal pointing fusarium oxysporum growth
With.
Background technique
By Fusarium oxysporum tomato specialized form (F.oxysporum f.sp lycopersici snyderet et
Hansen) infecting tomato wilt caused by tomato is a kind of global silborne fungal diseases.Fusarium oxysporum can generate thickness
Wall spore is one of the germ of a few energy Overwintering Growth, can long-term survival in the soil, and pathogenicity is strong, the death rate is big, disease
Bacterium enters vascular bundle from root invaded plants body, causes the obstruction of vascular bundle, hinders root and be delivered up water and nutrition
Substance causes plant to lack moisture and nutrition and death, is to threaten one of tomato yield and the Major Diseases of quality, passes through chemistry
Prevention and treatment, biological control, cultivation disease-resistant variety and some cultural control measures fail to the generation for being effectively prevented wilt disease, administer
It is extremely difficult.
There are many method of currently used prevention and control soil-borne disease of crop, wherein reduction soil disinfection method (Reductive by force
Soil Disinfestation, RSD) due to its efficient stable and environment friendly the advantages of, in the states such as the U.S. and Japan
It is widely used in agricultural production as chemical fumigant alternative.
Strong reduction sterilization emphasis is that sick soil is added in labile organic materials, and waterflooding, overlay film are created in a short time
Strong soil reduction process condition, issuable poisonous and harmful substance includes that acetic acid, propionic acid etc. are organic in anaerobic fermentation process
Acid and ammonia, hydrogen sulfide, bioactive substance etc. have grown the substance of inhibiting effect to Fusarium oxysporum, to reach killing
The purpose of soil-borne pathogen.
Currently, people are concentrated mainly on the different organic matters of exploration, and current research table for restoring the research of sterilization by force
The addition of bright corn stover significantly reduces the quantity of Fusarium oxysporum in soil, Fusarium oxysporum number in soil before only handling
The 2.88% of amount, but be still not up to ideal sterilization effect, therefore, with strong reduction sterilization kill Fusarium oxysporum to
In terms of administering wilt disease, it is badly in need of a kind of more effective modification method and application technology.
Summary of the invention
The present invention has for existing strong reduction sterilization is not thorough the killing effect of Fusarium oxysporum, falls dry metacone
Fusarium oxysporum still can again mass propagation the drawbacks of, Fusarium oxysporum can more thoroughly be killed by providing one kind, to effectively control
Manage the scheme of tomato wilt evil.Specifically, the present invention includes the following contents.
The first aspect of the present invention provides the composition for restoring soil disinfection by force, and it includes Yue Shi Flavobacteriums and glimmering
The cellulose degradation composite bacteria agent of light pseudomonad composition, the ratio between clump count of two kinds of effective bacterium are preferably 0.8~1.2:1, more
Preferably 1:1.
Optionally, composition of the invention further comprises corn stover, it is preferable that corn stalk powder is broken to 2cm or less
Size.
Preferably, it shakes when composition of the invention is contacted with fluid nutrient medium under the conditions of 28 DEG C, 150~180r/min
When swinging culture 72 hours, total living bacteria count is 3.7 × 10 in the liquid bacterial agent of acquisition9CFU/ml or more.Preferably, herein
Culture medium includes 3 parts by weight of beef extract, 5 parts by weight of peptone, 2 parts by weight of yeast extract, 8 parts by weight of glucose and agar powder 16
Parts by weight, the pH of fluid nutrient medium are 7.0 or less.
The second aspect of the present invention provides the method for inhibiting Fusarium oxysporum growth comprising make described in first aspect
The step of composition and Fusarium oxysporum for restoring soil disinfection by force contact.
The third aspect of the present invention provides the method for the strong reduction soil disinfection of improvement comprising make first party of the present invention
For restoring the step of composition of soil disinfection is contacted with soil by force described in face.
Preferably, the strong reduction soil disinfection of improvement of the invention method the following steps are included:
(1) soil to be processed is crossed into 5cm sieve, the composition for restoring soil disinfection by force is added to it, it is preferable that every thousand
The sum that the bacterium colony of microbial inoculum is originated from gram soil is 7.4 × 108CFU or more optionally makes the weight of corn stover in soil
2~10%；
(2) waterflooding did not had the soil, is sealed processing, that is, completes the improvement to sick soil.Preferably, encapsulation process
Temperature is 25~40 DEG C, and the processing time is 10~20 days.
The fourth aspect of the present invention provides the composition for restoring soil disinfection by force in anti-improvement tomato wilt
Using.
The cellulose degradation composite bacteria agent that the present invention adds has broken the original biological community structure of soil, is restoring by force
It can accelerate corn stover decomposition under environment, promote Corn Stalk Decomposition, improve ammonia, methane, organic acid and the vulcanization decomposed and generated
Hydrogen etc. further increases the effect for killing soil-borne pathogen to the concentration of the substance of the toxic effect of Fusarium oxysporum in a short time
Fruit.The Fusarium oxysporum that 99.9% can be killed through the invention still persistently kills 97.0% pathogen between falling dry spell.
Cellulose degradation composite bacteria agent of the invention can significantly improve the pH of acid soil, compared with singly applying corn stover
SO4 2-And NO3 -It is largely restored, NH4 +Significantly increase, the generation of soil secondary salinization can be effectively relieved.In addition, composite bacteria agent
In two kinds of bacterium synergistic effect obtain higher straw degradative rates, be much higher than the stalk of one or both of independent two kinds of bacterial strains adduction
Degradation rate.
The present invention breaks the original biological community structure of soil, Corn Stalk Decomposition is promoted to generate ammonia, methane, organic acid
There is the substance of toxic action with hydrogen sulfide etc. to Fusarium oxysporum, while the present invention can also neutralize soil pH, and soil is effectively relieved
Earth secondary salinization, increases soil fertility.Compared with the conventional method, the preferably additional organic matter corn stover of the present invention, raw material
It is at low cost, and can be applied to existing straw-returning technology and technique for producing organic fertilizer.
Can improve soil physico-chemical property after processed by the invention, kill soil-borne disease bacterium include but is not limited to Fusarium,
Pythium spp, phytophthora, verticillium dahliae and root-knot nematode, the effect in terms of administering soil soil-borne disease are significant.It can be significantly
The regulation effect of tomato wilt is improved, provides application foundation for wilt disease prevention and treatment, and further to strengthen waterflooding measure, enhancing
Control effect provides theories integration and technical support.
Detailed description of the invention
Fig. 1 present invention improves soil pH result schematic diagram.In Fig. 1, F0: on the day of waterflooding, the F5: waterflooding the 5th day, F10: waterflooding
It 10th day, F15: waterflooding the 15th day, Dry: falls dry；FC: waterflooding after addition corn stover, FCJ: addition corn stover and degradation bacteria
Waterflooding afterwards.
Fig. 2 present invention improves soil NH4 +Result schematic diagram.In Fig. 2, F0: on the day of waterflooding, F5: waterflooding the 5th day, F10: flood
It water the 10th day, F15: waterflooding the 15th day, Dry: falls dry；FC: waterflooding after addition corn stover, FCJ: addition corn stover and degradation
Waterflooding after bacterium.
Fig. 3 present invention improves soil SO4 2-Result schematic diagram.In Fig. 3, F0: on the day of waterflooding, F5: waterflooding the 5th day, F10: flood
It water the 10th day, F15: waterflooding the 15th day, Dry: falls dry；FC: waterflooding after addition corn stover, FCJ: addition corn stover and degradation
Waterflooding after bacterium.
Sterilization effect schematic diagram of Fig. 4 present invention to soil Fusarium oxysporum.In Fig. 4, F0: on the day of waterflooding, F5: waterflooding the
It 5 days, the F10: waterflooding the 10th day, F15: waterflooding the 15th day, Dry: falls dry；FC: waterflooding after addition corn stover, FCJ: addition corn
Waterflooding after stalk and degradation bacteria.
Specific embodiment
The existing various exemplary embodiment that the present invention will be described in detail, the detailed description are not considered as to limit of the invention
System, and it is understood as the more detailed description to certain aspects of the invention, characteristic and embodiment.
It should be understood that it is to describe special embodiment that heretofore described term, which is only, it is not intended to limit this hair
It is bright.In addition, for the numberical range in the present invention, it is thus understood that specifically disclose the range upper and lower bound and they it
Between each median.Median and any other statement value in any statement value or stated ranges or in the range
Lesser range is also included in the present invention each of between interior median.These small range of upper and lower bounds can be independent
Ground includes or excludes in range.
Unless otherwise stated, all technical and scientific terms used herein has the routine in field of the present invention
The normally understood identical meanings of technical staff.Although the present invention only describes preferred method and material, of the invention
Implement or also can be used and similar or equivalent any method and material described herein in testing.The institute mentioned in this specification
There is document to be incorporated by reference into, to disclosure and description method relevant to the document and/or material.It is incorporated to any
When document conflicts, it is subject to the content of this specification.Unless otherwise stated, " % " is the percentage based on weight.
In the examples where no specific technique or condition is specified, described technology or conditions according to the literature in the art,
Or it is carried out according to product description.Reagents or instruments used without specified manufacturer, be can be by regular channel commercially available from
Available conventional products.
Embodiment 1
The present embodiment is the preparation example for restoring the composition of soil disinfection by force, the specific steps of which are as follows:
(1) cellulose degradation composite bacteria agent
Take Yue Shi Flavobacterium (Flavobacterium johnsoniae) (laboratory preservation) and fluorescence respectively with oese
Each ring bacterial strain access LB culture medium of pseudomonad (Pseudomonasfluorescens Migula) (laboratory preservation) is successively
It carries out the activation of two single bacteriums (as control) and combination microbial inoculum and expands culture experiment, 28 DEG C, shake under the conditions of 150r/min
Culture 24 hours；By volume 1:1 draw activation after bacterium solution into corresponding liquid fermentation medium, 28 DEG C of temperature,
Liquid bacterial agent seed liquor is obtained to logarithmic growth phase within shake culture 72 hours under the conditions of 150r/min；Spread plate method obtains total
Living bacteria count is 3.7 × 109CFU/ml or more.
(2) for the acquisition of examination corn stover
The corn stover of crushing is crossed into 20 meshes, 105 DEG C drying to constant weight, spare.
(3) for restoring the composition of soil disinfection by force
The above-mentioned stalk of 10g, 121 DEG C of high pressure sterilization 20min are separately added into three triangular flasks.Add respectively in each triangular flask
Enter the bacteria suspension of 150mL Yue Shi Flavobacterium, Pseudomonas fluorescens and composite bacteria agent of the invention.
Two kinds of bacterial strains and combinations thereof are 3 groups of parallel, 120r/min respectively, after 28 DEG C of culture 20d, by culture 5000r/
Min is centrifuged 10min, abandons supernatant, with the mixed liquor repeated flushing of dilute hydrochloric acid and dust technology to removing thallus, 5000r/min from
Heart 10min abandons supernatant, then uses distilled water flushing, and 5000r/min is centrifuged 10min, and 105 DEG C drying to constant weight, calculates each list
Bacterium and composite bacteria agent cultivate the degradation effect after 20d to corn stover at 28 DEG C.The straw degradative of two plants of single bacteriums and composite bacteria agent
Rate is as shown in table 1.
The straw degradative rate (20 days, corn stover) of two plants of single bacteriums of table 1- and composite bacteria agent
Embodiment 2
The present embodiment is that the bacterium of tomato wilt pathogen Fusarium oxysporum is gone out effect test, and the method for the present embodiment includes
Following steps:
1. testing soil basic condition
The Shenyang City, Liaoning Province Shujiatun District village Song great Tai is picked up from, is 15 years greenhouses of tomato pakchoi crop rotation, this area is certainly
Disease is serious within 2010, and disease incidence is 60.0%~70.0%.Maturity period, every Gansu Province only remained 6~10 plants of plant, and the underproduction is serious.
It is brown earth, pH 5.29, Eh 408.60mV, full carbon 4.04%, full nitrogen 0.47%, ammonium nitrogen for examination soil
31.41mg·kg-1, nitrate nitrogen 297.70mgkg-1, sulfate radical 890.85gkg-1, Fusarium oxysporum number is 5.16CFU
g-1Soil.
2. for the acquisition for trying corn stover
Taking 105 DEG C of corn stover, drying to constant weight, crushes the fritter for 1cm or so, and fundamental property includes: full carbon
40.50%, full nitrogen 1.10%.
3. test process
1) sick soil is fetched into 5cm sieve, and was packed into the bucket that drainage hole is arranged at diameter 30cm, high 70cm and bottom, and dispense every barrel
10kg soil (blocking bottom drain holes).The corn stover of soil quality 2% is added, cellulose degradation 200ml of the invention is multiple
Combined bacteria agent, stirs evenly；Corn stover is only added in control group (FC).
2) waterflooding there was not soil 5cm, and preservative film sealing, sampling in every 5 days is primary, handles 15 days, removes top preservative film, opens
The drainage hole of bucket bottom, soil natural, which is fallen, to be done to field capacity.
4. soil physical chemistry measures
Soil pH (soil ratio 5:1) is using DMP-2pH meter (Quark Ltd, China) measurement, SO4 2-Content (soil ratio 5:
1) barium sulfate colorimetric method for determining, NH are used4 +Content using San++ Continuous Flow Analysis instrument (Skalar, Breda,
Netherlands it) measures.Measurement result is referring to Fig. 1 to Fig. 3.
5. the measurement of Fusarium oxysporum quantity in soil
The measurement reference literature of Fusarium oxysporum: Peng Shuan, Wang Yiming, Ye Xuhong et al., Soil Factors are to pathogenic
Influence [J] soil of Fusarium oxysporum growth, 2014 (5): method disclosed in 845-850. carries out.The results are shown in Table 2:
The bacterium of table 2- tomato wilt pathogen Fusarium oxysporum is gone out effect
It is the control group for only adding corn stover that FC is handled in table 2, and FCJ is embodiment 1 for restoring soil disinfection by force
Composition.The present invention to the sterilization effect of soil Fusarium oxysporum referring to fig. 4.
Embodiment 3
The present embodiment is that the influence for carrying out tomato disease incidence and yield with the composition that embodiment 1 obtains is tested, this implementation
Example method the following steps are included:
1. testing soil basic condition
The Shenyang City, Liaoning Province Shujiatun District village Song great Tai is picked up from, is 15 years greenhouses of tomato pakchoi crop rotation, this area is certainly
Disease is serious within 2010, and disease incidence is 60.0%~70.0%.Maturity period, every Gansu Province only remained 6~10 plants of plant, and the underproduction is serious.
It is brown earth, pH 5.29, Eh 408.60mV, full carbon 4.04%, full nitrogen 0.47%, ammonium nitrogen for examination soil
31.41mg·kg-1, nitrate nitrogen 297.70mgkg-1, sulfate radical 890.85gkg-1, Fusarium oxysporum number is 5.16CFU
g-1Soil.
2. for studying object: tomato, kind are green Taro.
3. test process
1) sick soil is fetched into 5cm sieve, and was packed into the bucket that drainage hole is arranged at diameter 30cm, high 70cm and bottom, and dispense every barrel
10kg soil (blocking bottom drain holes).The corn stover of soil quality 2% is added, cellulose degradation 200ml of the invention is multiple
Combined bacteria agent, stirs evenly；Corn stover is only added in control group (FC).
2) waterflooding there was not soil 5cm, and preservative film sealing is handled 15 days, removes top preservative film, opens the draining of bucket bottom
Hole, soil natural, which is fallen, to be done to field capacity, is transplanted form and almost the same 1 plant of tomato of growing way, is carried out normal liquid manure pipe
Reason.
4. influence test of the strong reduction soil disinfection method of improvement to eggplant disease incidence and yield
Disease incidence evaluation criteria
Disease incidence=morbidity strain number/test total strain number
1 grade: disease-free spot；2 grades: lesion area accounts for the 0%~5.0% of entire blade face；3 grades: lesion area accounts for entire blade face
6.0%~25.0%；4 grades: lesion area accounts for the 26.0%~50.0% of entire blade face；5 grades: lesion area accounts for entire blade face
50% or more.The results are shown in Table 3:
The influence of table 3- tomato disease incidence and yield is tested
It is the control group for only adding corn stover that FC is handled in table 3, and FCJ is embodiment 1 for restoring soil disinfection by force
Composition.
Embodiment 4
The present embodiment is the influence test that the improvement object combination obtained with embodiment 1 carries out Tomato Quality, the present embodiment
Method the following steps are included:
1. testing soil basic condition
The Shenyang City, Liaoning Province Shujiatun District village Song great Tai is picked up from, is 15 years greenhouses of tomato pakchoi crop rotation, this area is certainly
Disease is serious within 2010, and disease incidence is 60.0%~70.0%.Maturity period, every Gansu Province only remained 6~10 plants of plant, and the underproduction is serious,
It is brown earth, pH 5.29, Eh 408.60mV, full carbon 4.04%, full nitrogen 0.47%, ammonium nitrogen for examination soil
31.41mg·kg-1, nitrate nitrogen 297.70mgkg-1, sulfate radical 890.85gkg-1, Fusarium oxysporum number is 5.16CFU
g-1Soil.
2. for studying object: tomato, kind are green Taro.
3. test process
1) sick soil is fetched into 5cm sieve, and was packed into the bucket that drainage hole is arranged at diameter 30cm, high 70cm and bottom, and dispense every barrel
10kg soil (blocking bottom drain holes).The corn stover of addition soil quality 2%, the cellulose degradation composite bacteria agent of 200ml,
It stirs evenly；Corn stover is only added in control group (FC).
2) waterflooding there was not soil 5cm, and preservative film sealing is handled 15 days, removes top preservative film, opens the draining of bucket bottom
Hole, soil natural, which is fallen, to be done to field capacity, is transplanted form and almost the same 1 plant of tomato of growing way, is carried out normal liquid manure pipe
Reason.
4. Tomato Quality is tested
Organic acid: phenolphthalein-sodium hydroxide titration
Vc:2,6- dichloropheno-lindophenol method
Soluble sugar: anthrone method
Total Soluble Sugar: the concentrated sulfuric acid-anthrone ethyl acetate colorimetric method.
As a result as table 4 shows:
The influence of table 4- Tomato Quality is tested
It is the control group for only adding corn stover that FC is handled in table 4, and FCJ is embodiment 1 for restoring soil disinfection by force
Composition.
Without departing substantially from the scope or spirit of the invention, the specific embodiment of description of the invention can be done more
Kind improvements and changes, this will be apparent to those skilled in the art.Other realities obtained by specification of the invention
Applying mode for technical personnel is apparent obtain.Present specification and embodiment are merely exemplary.
Claims (10)
1. a kind of for restoring the composition of soil disinfection by force, which is characterized in that the composition include by Yue Shi Flavobacterium and
The cellulose degradation composite bacteria agent of Pseudomonas fluorescens composition.
2. according to claim 1 for restoring the composition of soil disinfection by force, which is characterized in that the Yue Shi Flavobacterium
It is 0.8~1.2:1 with the ratio between the clump count of the Pseudomonas fluorescens.
3. according to claim 2 for restoring the composition of soil disinfection by force, which is characterized in that further comprise crushing
To the corn stover of 2cm or less size.
4. according to claim 3 for restoring the composition of soil disinfection by force, which is characterized in that when by the composition
Fluid nutrient medium is contacted under the conditions of 28 DEG C, 150~180r/min at shake culture 72 hours, it is total in the liquid bacterial agent of acquisition
Living bacteria count is 3.7 × 109CFU/ml or more.
5. according to claim 4 for restoring the composition of soil disinfection by force, which is characterized in that the fluid nutrient medium
Include 3 parts by weight of beef extract, 5 parts by weight of peptone, 16 weight of 2 parts by weight of yeast extract, 8 parts by weight of glucose and agar powder
Part, and the pH of the fluid nutrient medium is 7.0 or less.
6. a kind of method for inhibiting Fusarium oxysporum growth, which is characterized in that including making any one institute according to claim 1~5
The step of composition for restoring soil disinfection by force stated is contacted with Fusarium oxysporum.
7. a kind of method of the strong reduction soil disinfection of improvement, which is characterized in that including making any one according to claim 1~5
The step of composition for restoring soil disinfection by force is contacted with soil.
8. the method for the strong reduction soil disinfection of improvement according to claim 7, which comprises the following steps:
(1) soil to be processed is crossed into 5cm sieve, is added to it described in any item for going back native soil by force according to claim 1~5
The composition of sterilizing, so that the sum of the bacterium colony in every kilogram of soil from microbial inoculum is 7.4 × 108CFU or more optionally makes
The weight of corn stover is 2~10% in soil；
(2) waterflooding did not had the soil, is sealed processing, that is, completes the improvement to sick soil.
9. the method for the strong reduction soil disinfection of improvement according to claim 8, which is characterized in that the encapsulation process
Temperature is 25~40 DEG C, and the processing time is 10~20 days.
10. described in any item compositions for restoring soil disinfection by force are withered in anti-improvement tomato according to claim 1~5
Application in disease.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112458021A (en) * | 2020-12-07 | 2021-03-09 | 江苏省农业科学院 | Biocontrol pseudomonas fluorescens and culture method and application thereof |
| CN112458021B (en) * | 2020-12-07 | 2022-07-01 | 江苏省农业科学院 | Biocontrol pseudomonas fluorescens and culture method and application thereof |
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