CN109020699A - Soil remediation leavening, soil remediation microbial inoculum, preparation method and soil remediation method - Google Patents
Soil remediation leavening, soil remediation microbial inoculum, preparation method and soil remediation method Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 221
- 238000005067 remediation Methods 0.000 title claims abstract description 73
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 58
- 241000894006 Bacteria Species 0.000 claims abstract description 49
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 49
- GNTDGMZSJNCJKK-UHFFFAOYSA-N divanadium pentaoxide Chemical compound O=[V](=O)O[V](=O)=O GNTDGMZSJNCJKK-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000000126 substance Substances 0.000 claims abstract description 40
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- 239000004615 ingredient Substances 0.000 claims abstract description 20
- 150000003839 salts Chemical class 0.000 claims abstract description 19
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- 229910052791 calcium Inorganic materials 0.000 claims abstract description 18
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- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims abstract description 15
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- GPPXJZIENCGNKB-UHFFFAOYSA-N vanadium Chemical compound [V]#[V] GPPXJZIENCGNKB-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Soil Sciences (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The present invention discloses a kind of soil remediation leavening, soil remediation microbial inoculum, preparation method and soil remediation method.Wherein, soil remediation leavening, ingredient including following parts by weight: 15-25 parts of calcium lime powder, 15-25 parts of brown sugar, 40-60 parts of edible salt, 20-40 parts of sodium bicarbonate, 40-60 parts of ruderal ash, 40-60 parts of sawdust ash, 0.1-0.4 parts of vanadic anhydride, 40-60 parts of rice straw powder, soil 1-60 parts to be administered.Using soil remediation leavening of the invention, it is capable of the strong characteristic of the resistance of dominant strain of the effectively stable and activation energy in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution with growth vigor, will not making strain resistance because of preservation time length, equal growth vigor generates degeneration by force, has in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution the dominant bacteria with growth vigor with obvious action to preservation.
Description
Technical field
The present invention relates to arable soils to administer field, and in particular to soil remediation leavening, soil remediation microbial inoculum, its preparation
Method and soil remediation method.
Background technique
China is that a ground adult is more but serious unbalanced and total amount wretched insufficiency the country of cultivated area, shared per capita to plough
Ground area lags far behind world's per capita share, just in this case, the hardened and agriculture chemical and heavy metal of a large amount of soil
Seriously polluted exceeded close to the limit has been even up to, only heavy metals exceeding standard is to reach 5000hm, and comprehensive pollution object is exceeded to be reached
Nearly the 20% of cultivated area is accounted for 400,000,000 mu, comprehensive pollution object is bigger close to exceeded area！Seriously threaten food safety namely state
The safety of people's health is administered extremely urgent.
As rapid development of economy and long-term environmental consciousness are thin, various industrial wastewaters, exhaust gas, domestic waste and
Without being effectively treated i.e. a large amount of discharge beyond standards, chemical fertilizer, pesticide, antibiotic etc. transfinite application and violated for sewage, aquaculture wastewater etc.
It uses, is seriously polluted caused by soil, especially heavy metal pollution, endanger day caused by human residential environment and health
It is beneficial serious；Although country and each place all improve environment, pollution administration trying one's best, various administering methods, Duo Shuoxiao
Fruit is undesirable, occurs the hardened advanced technology with pollution of biological treating arable soil in recent years, but since biotechnology is inadequate
Mature, at high cost, low efficiency, effect are unstable etc., can only excessive technology at last.
Summary of the invention
The application provides a kind of soil remediation leavening, soil remediation microbial inoculum, preparation method and soil remediation method.
In order to achieve the above objectives, the application uses following technical scheme:
According in a first aspect, the application discloses a kind of soil remediation leavening, the ingredient including following parts by weight: quick lime
15-25 parts of powder, 15-25 parts of brown sugar, 40-60 parts of edible salt, 20-40 parts of sodium bicarbonate, 40-60 parts of ruderal ash, sawdust ash 40-
60 parts, 0.1-0.4 parts of vanadic anhydride, 40-60 parts of rice straw powder, soil 1-60 parts to be administered.
Preferably, soil remediation leavening includes the ingredient of following parts by weight: 18-23 parts of calcium lime powder, brown sugar 18-23
Part, 45-55 parts of edible salt, 25-35 parts of sodium bicarbonate, 45-55 parts of ruderal ash, 45-55 parts of sawdust ash, vanadic anhydride 0.2-
0.3 part, 45-55 parts of rice straw powder, soil 1-30 parts to be administered.
It is furthermore preferred that soil remediation leavening includes the ingredient of following parts by weight: 20 parts of calcium lime powder, 20 parts of brown sugar, food
With 50 parts of salt, 30 parts of sodium bicarbonate, 50 parts of ruderal ash, sawdust are 50 parts grey, and 0.2 part of vanadic anhydride, 50 parts of rice straw powder, wait control
Soil 1-30 parts of reason.
Most preferably, soil to be processed is the bacterium solution of the dominant strain screened in soil to be processed.
According to second aspect, the application discloses a kind of side for preparing soil remediation leavening disclosed in the application first aspect
Method weighs the following raw material by formula components weight ratio: 15-25 parts of calcium lime powder, 15-25 parts of brown sugar, and 40-60 parts of edible salt,
20-40 parts of sodium bicarbonate, 40-60 parts of ruderal ash, 40-60 parts of sawdust ash, 0.1-0.4 parts of vanadic anhydride, rice straw powder 40-60
Part；Then soil 1-60 parts of mixing to be processed are taken again, and soil remediation leavening is made.
According to the third aspect, the application discloses a kind of soil remediation microbial inoculum, including soil disclosed in the application first aspect
The leavening of the preparation of soil treatment leavening preparation method disclosed in leavening or the application second aspect is handled, by weight,
Ingredient including following ratio is protected from light fermentation through constant temperature and humidity anaerobism and obtains: rice straw powder 55%-65%, wood dust ash 10%-
20%, ruderal ash 20%-30%, leavening 0.1%-2%.
Preferably, soil remediation microbial inoculum, by weight, the ingredient including following ratio: rice straw powder 60.5%, wood dust ash
15%, ruderal ash 24%, leavening 0.5%.
According to fourth aspect, the application discloses a kind of side for preparing soil remediation microbial inoculum disclosed in the application third aspect
Method includes the following steps:
Raw material preparation: raw material: rice straw powder 55%-65%, wood dust ash 10%- is configured by following component weight ratio
20%, ruderal ash 20%-30%, leavening 0.1%-2%；
Prepare microbial inoculum: it is first that the leavening of 0.1%-2% is soluble in water, then pour into the above ratio other raw materials mixing stir
Mixing is mixed, constant temperature and humidity anaerobism is placed in and is protected from light fermentation in fermenting cellar, obtain the soil remediation microbial inoculum.
Preferably, fermentation time is 10-30 days.
It is furthermore preferred that fermentation time is 15-25 days.
Most preferably, fermentation time is 18 days.
According to the 5th aspect, the application disclose it is a kind of using soil remediation leavening disclosed in the application first aspect or this
Apply soil remediation microbial inoculum disclosed in the third aspect in the soil for administering soil hardening and agriculture chemical heavy metal comprehensive pollution
Utilization.
According to the 6th aspect, the application discloses a kind of method for administering soil, method are as follows: by weight by the application third
The soil remediation of the preparation method preparation of soil remediation microbial inoculum disclosed in soil remediation microbial inoculum or fourth aspect disclosed in aspect
Microbial inoculum 80%-90% and plant ash 10%-20% is mixed, and is applied in upper soll layer to be administered；Then farming soil to be administered
The mixture of microbial inoculum and plant ash is uniformly distributed in the soil, paddy field 20 days or so, nonirrigated farmland 40-50 days or so are controlled
Manage the soil of improvement.
Due to using the technology described above, the beneficial effects of the present application are as follows:
1. use soil remediation leavening of the invention, can effectively stable and activation energy in soil hardening and pesticide
The strong characteristic of the resistance of dominant strain in the soil of fertile heavy metal comprehensive pollution with growth vigor, will not be because of the preservation time
Growth vigor long and that strain resistance is waited by force generates degeneration, has preservation in soil hardening and agriculture chemical weight
Dominant bacteria in the soil of metal comprehensive pollution with growth vigor has obvious action.
2. soil remediation microbial inoculum of the invention efficiently quickly can thoroughly decompose the residual such as agriculture chemical heavy metal in soil,
Solve to accumulate because of superposition throughout the year transfinite apply the reasons such as chemical fertilizer caused by soil hardening, rehabilitating soil microbial ecosystem is extensive
The original microbial ecosystem balance of earth backing earth, promotes the normal growth of various crops, improves crop yield and quality,
Ensure that there is no pesticides and heavy-metal residual in grain and other various food materials medicinal materials, fundamentally solve pesticide and heavy metal is residual
It takes a photograph and rings the problem of compatriots' health.Therefore the dual substantial worth with apparent environmental protection and maintenance human health.
3. administering soil hardening and agriculture chemical heavy metal using soil remediation leavening of the invention, soil remediation microbial inoculum
The method of the soil of comprehensive pollution is mildly high-efficient, and effect at low cost is high, and main raw material(s) is rice straw powder, ruderal powder, wood
Consider powder, plant ash, calcium lime powder, edible salt, the low values article such as sodium bicarbonate to be worth doing, annual output is huge be it is inexhaustible with it not
It exhausts, maximum demand can be met, be not only easy to get, the harmful competition of resource is also not present, be conducive to industrialization rule
Mould production and extensive soil of administering are promoted the use of；The micro- fertilizer application this season of the super lactic acid bacteria of auxotype can reach best and administer effect
Fruit, quick, grain or other crop yield high-quality are good, are easy to implement, are easy to promote, and extension process does not have any technology
Obstacle.
Specific embodiment
Below by specific embodiment, invention is further described in detail.
Soil remediation microbial inoculum of the present invention is different from various microbial manures and microbial nutrient solution in the market.Now
Microbial manure and microbial nutrient solution are many kinds of in the market, are all as obtained by the artificial culture of culture medium progress, strain is
From the market or other biological laboratory and store microorganism scientific research institutions purchase.In a large amount of research and practice, this
Inventor is stored in the microbial population of each laboratory and scientific research institutions, in the prevalence of degeneration by investigation test discovery
Serious phenomenon.The life condition of microbial flora is extremely harsh to environmental requirement, and different microorganisms is to environment temperature, wet
The material requirement that degree, nutrition etc. are depended on is different, the existing Techniques of preserving of the mankind and method, and microorganism storage is much not achieved and protects
Active requirement is held, this seriously constrains microbial biochemical, breeding and is metabolized this important natural law.Resting period is longer,
Spawn degeneration is more serious, generally existing using such Spawn incubation and the microbial manure and various nutrient solutions that prepare
Preparation cost is high, service efficiency is low, to control of desert effect difference or unstable, situations such as can not being widely applied, Bu Nengcong
Fundamentally solve the problems, such as.
The present invention creatively uses calcium lime powder, brown sugar, edible salt, sodium bicarbonate, ruderal ash, sawdust ash, five oxygen
Change two vanadium, rice straw powder is made into fermentation medium, then by the soil of soil hardening to be processed and agriculture chemical heavy metal comprehensive pollution
Earth is added in starter medium, contains energy in the soil of soil hardening to be processed and agriculture chemical heavy metal comprehensive pollution
The strong dominant strain of the resistance grown in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution.The fermented and cultured
In base, brown sugar, edible salt, sodium bicarbonate, ruderal ash, sawdust ash and rice straw powder are provided jointly needed for dominant strain growth and breeding
Substances, the calcium lime powder such as carbon source, nitrogen source, inorganic salts, water provide Ca for culture medium+, meanwhile, in the process for preparing culture medium
In, calcium lime powder is reacted with water can be heated beyond 300 DEG C of high temperature, which can make other microorganisms in medium component
Inactivation.Calcium lime powder is reacted with water generates Ca (OH)2, Ca (OH)2So that culture medium is in alkalinity, so that the culture medium of preparation is in
Stressful environmental, with the advantage of growth vigor in the soil for being maintained at soil hardening and agriculture chemical heavy metal comprehensive pollution
The strong characteristic of the resistance of bacterial strain.Vanadic anhydride has toxic effect to microorganism, and the present inventor is in long-term section
It grinds and is found in practicing, a small amount of vanadic anhydride has stable and activation energy in landfill leachate or reverse osmosis concentrated liquid sewage
The resistant characteristic of the resistant dominant strain of middle growth, it is extremely critical to saving and maintaining resistant dominant strain to have the function of.Cause
There is toxic effect to microorganism for vanadic anhydride, so the amount of vanadic anhydride cannot be excessive in fermentation medium, excessively
Resistant dominant strain can be inactivated, can not be very little, stable and activation energy is not had very little in landfill leachate or reverse osmosis concentrated
The effect of the resistant characteristic of the resistant dominant strain grown in contracting liquid sewage, in formula of the invention, using vanadic anhydride
0.1-0.5 parts can play good effect, be better effect, 0.3 part of vanadic anhydride tool using 0.2-0.4 parts of vanadic anhydride
There is best effect.
The content of each ingredient of above-mentioned starter medium can change in a wider scope.It is excellent in of the invention one
It selects in embodiment, the ingredient including following parts by weight: 15-25 parts of calcium lime powder, 15-25 parts of brown sugar, 40-60 parts of edible salt,
20-40 parts of sodium bicarbonate, 40-60 parts of ruderal ash, 40-60 parts of sawdust ash, 0.1-0.4 parts of vanadic anhydride, rice straw powder 40-60
Part, soil 1-60 parts to be administered.
In one further preferred embodiment, the ingredient including following parts by weight: 18-23 parts of calcium lime powder, brown sugar 18-23
Part, 45-55 parts of edible salt, 25-35 parts of sodium bicarbonate, 45-55 parts of ruderal ash, 45-55 parts of sawdust ash, vanadic anhydride 0.2-
0.3 part, 45-55 parts of rice straw powder, soil 1-30 parts to be administered.
In one further preferred embodiment, the ingredient including following parts by weight: 20 parts of calcium lime powder, 20 parts of brown sugar, food
With 50 parts of salt, 30 parts of sodium bicarbonate, 50 parts of ruderal ash, sawdust are 50 parts grey, and 0.2 part of vanadic anhydride, 50 parts of rice straw powder, wait control
Soil 1-30 parts of reason.
For more optimized leavening, in a preferred embodiment, in soil hardening to be processed and agriculture chemical weight
Dominant strain is filtered out in the soil of metal comprehensive pollution, then the bacterium solution of dominant strain is replaced into soil hardening to be processed and agriculture
The soil of medicine chemical fertilizer heavy metal comprehensive pollution, which is added in fermentation medium, collectively constitutes leavening.
The dominant bacteria that the present invention screens is the resistance in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution
The strong lactic acid bacteria with growth vigor, lactic acid bacteria have improvement soil property, increase soil fertility, inhibit such as spoilage organisms nocuousness
The growth and breeding of microorganism, enhancing plant metabolism function: promoting germination and well developed root system to enhance plant growth ability,
Improve photosynthetic effect.The lactic acid bacteria that the screenings such as molten calcium circle method, bromocresol green indicator method have growth vigor can be used.
The screening technique of dominant strain are as follows:
The configuration of screening and culturing medium: beef extract 10g/L, peptone 10g/L, yeast extract 10g/L, tomato juice 200g/L are spat
Temperature 0.05%, glucose 10g/L, calcium carbonate 15-20g/L, bromocresol green 0.01%, pH 6.0-6.5, agar 10%, high temperature are high
Pressure sterilizing 20min, pours into culture dish and is configured to solid screening and culturing medium.
Inoculation: 10ml sterile water is added in the soil sample of soil hardening 1g to be administered and agriculture chemical heavy metal comprehensive pollution
In be configured to soil suspension, then be configured to 10 times of diluted serial suspensions that successively decrease, take dilution 10-4-10-6Suspension is each
80-100uL is respectively coated in screening and culturing medium, is placed in incubated at room temperature 2-6 days.
Obtain have growth vigor lactic acid bacteria: lactic acid bacteria grow up to after on culture medium displaing yellow, later can thin out black,
It marks the lactic acid bacteria grown at first and 4-6 days bacterium colonies of culture is maximum and the optimal lactic acid bacteria of rising trend is the cream with growth vigor
Sour bacterium, conservation are used to prepare leavening use.
The present invention discloses the preparation method of the leavening of preparation treatment soil in second aspect, by formula components weight ratio
Example weighs the following raw material: 15-25 parts of calcium lime powder, 15-25 parts of brown sugar, 40-60 parts of edible salt, 20-40 parts of sodium bicarbonate, wild miscellaneous
40-60 parts of plant ash, 40-60 parts of sawdust ash, 0.1-0.4 parts of vanadic anhydride, 40-60 parts of rice straw powder；Then it takes again to be processed
1-60 parts of soil mixing, are made soil remediation leavening.It should be noted that in the soil hardening that strain, that is, to be processed is added and
Before the soil of agriculture chemical heavy metal comprehensive pollution, the mixing of each raw material is soluble in water, and each to be restored to room temperature originally.
In a preferred embodiment, the soil hardening and agriculture chemical heavy metal comprehensive pollution of strain, that is, to be processed
Soil has by force growth excellent with the above-mentioned resistance screened from the soil sample of soil hardening and agriculture chemical heavy metal comprehensive pollution
The lactic acid bacterial liquid of gesture replaces.
The third aspect of the present invention discloses a kind of soil remediation microbial inoculum, and soil remediation microbial inoculum includes rice straw powder, wood dust
Mentioned component is protected from light by anaerobism and ferments by ash, the leavening of ruderal ash and above-mentioned preparation.It is of the present invention
The raw materials such as rice straw powder, wood dust ash, ruderal ash are that the present inventor's science from a large amount of raw material is detected, compared, filtering out
Optimal raw material because of the sugar rich in such as rice straw powder be that lactic acid bacteria most preferably depends on object, by especially fermenting, lactic acid bacteria
Activity, mass propagation can be improved rapidly herein, become advantage lactobacillus, and agriculture chemical hardened to improvement arable soil
Heavy metal comprehensive pollution, which improves Crops production and quality, unique, superpower effect.
The above-mentioned content for preparing each ingredient of microbial inoculum can change in a wider scope.It is preferred real at of the invention one
It applies in scheme, by weight, the ingredient including following ratio: rice straw powder 55%-65%, wood dust ash 10%-20% are wild miscellaneous
Plant ash 20%-30%, leavening 0.1%-2%.The present inventor is sought by widely screening, the detection of science, stringent comparison
The kinds of lactobacillus for looking for original lactic acid bacteria to depend in which plant is relatively more, biochemistry is bred and metabolism is than comparatively fast stablizing, having
There is high activity, most suitable as the culture medium of super dry powder lactic acid bacteria, concludes that rice straw powder, reed powder, sawdust in practice
The sugar contents such as powder, ruderal powder are enriched, depend on kinds of lactobacillus mostly and breeding is stablized fastly, activity is high, are to prepare nutrition
The natural quality raw materials of the super lactic acid bacteria microbe fertilizer of type.In the formula, the ratio between each component is by being incorporated in soil
The growth characteristics of lactic acid bacteria in the hardened soil with agriculture chemical heavy metal comprehensive pollution and the spy of each component nutritional ingredient
It puts and passes through a large amount of experimental verification and obtain, which expands culture in soil hardening and agriculture chemical heavy metal comprehensive pollution
In soil, extracts each component during there is growth vigor lactic acid bacteria and generate synergistic effect, enable above-mentioned advantage lactic acid bacteria
Activity is improved rapidly, and keeps its activity, and mass propagation becomes the above-mentioned lactobacillus with growth vigor.
In a preferred embodiment, soil remediation microbial inoculum, by weight, the ingredient including following ratio: rice straw powder
60.5%, wood dust ash 15%, ruderal ash 24%, leavening 0.5%.
The application discloses the method for preparing soil remediation microbial inoculum, step in fourth aspect are as follows:
Raw material preparation: raw material: rice straw powder 55%-65%, wood dust ash 10%- is configured by following component weight ratio
20%, ruderal ash 20%-30%, leavening 0.1%-2%；
Prepare microbial inoculum: it is first that the leavening of 0.1%-2% is soluble in water, then pour into the above ratio other raw materials mixing stir
Mixing is mixed, is placed in the sealed fermenting room that constant temperature and humidity anaerobism is protected from light and ferments, temperature is controlled at 35-37 DEG C, relatively wet
Degree control can ferment 10-30 days to avoid the pollution of miscellaneous bacteria in 80-90%, closed fermenting cellar, obtain the soil remediation bacterium
Agent.
In one preferred embodiment, it is placed in intelligentized control method constant temperature and humidity anaerobism and is protected from light fermentation 18 days in fermenting cellar.
It should be noted that the strain source of soil remediation leavening of the present invention and soil remediation microbial inoculum is soil to be administered
The hardened soil with agriculture chemical heavy metal comprehensive pollution of earth, or it is comprehensive in soil hardening to be administered and agriculture chemical heavy metal
That screens in contaminated soil has growth vigor lactic acid bacteria, soil hardening to be administered and agriculture chemical heavy metal comprehensive pollution
Soil in will necessarily exist be suitble to grow in the soil of soil hardening to be administered and agriculture chemical heavy metal comprehensive pollution
And carbon source in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution to be administered, nitrogen source, inorganic can be utilized
The ingredients such as salt, sugar carry out the lactic acid bacteria culturers of growth and breeding, so either being directlyed adopt using method of the invention wait control
The source of the soil hardening of reason and the soil of agriculture chemical heavy metal comprehensive pollution as microorganism, is also utilized in be administered
The lactic acid bacteria screened in the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution, with leavening and microbial inoculum of the invention
Formula, can get the soil remediation leavening for effectively administering the soil of soil hardening and agriculture chemical heavy metal comprehensive pollution and
Soil remediation microbial inoculum, and the characteristic of strong, with growth vigor the lactic acid bacteria of resistance can be protected steadily in leavening and microbial inoculum
It holds, degeneration will not be generated because of the time of storage length, also can be prepared into and have active high, stability after meeting water and humid air
By force, the super lactobacillus for breeding the features such as fast, is core of the invention technology, this technology be at present both at home and abroad most before
The technology on edge, tip.
The invention also discloses administering soil hardening using soil remediation leavening or soil remediation microbial inoculum of the invention
With the utilization in the soil of agriculture chemical heavy metal comprehensive pollution.
The invention also discloses use soil remediation microbial inoculum of the invention in the method for administering soil, by weight by this Shen
Please soil remediation microbial inoculum disclosed in soil remediation microbial inoculum or fourth aspect disclosed in the third aspect preparation method preparation soil
Earth is administered microbial inoculum 80%-90% and plant ash 10%-20% and is mixed, and applies in upper soll layer to be administered；Then farming waits controlling
The mixture of microbial inoculum and plant ash is uniformly distributed in the soil by reason soil, paddy field 20 days or so obtains the soil for administering improvement
Earth.
In a preferred embodiment, dryland soil improvement time is 40-50 days.
Using soil remediation microbial inoculum of the invention, the microorganism in soil can be made to accelerate mass propagation, efficiently improve plate
The soil of knot, decompose soil median year superposition accumulation application of transfiniting agriculture chemical and the violated antibiotic used, it is various come
The heavy metal bring comprehensive pollution of source deposition；And the various crops planted, pesticide is not used, chemical fertilizer and compound fertilizer are reduced
Usage amount, overall cost reduces, and grain or other crop yields improve 20-30%, and quality is greatly improved close to organic food
Product, and substantially surmount pollution-free food and green food.Above method continuous administration 3 years, that is, it can reach the mesh of the above radical cure
Mark；If rationally scientific agriculture soil, the soil through administering can keep this achievement for a long time later；If cultivating procedure does not obtain
It effectively instructs and soil quality is caused to degenerate again, repeat to administer again according to above-mentioned method；If this administering method is transported
Grain or the yield and quality of other crops are improved with the proper planting cost that significantly reduces, all the year round reasonably combined this hair of application
Bright microbial inoculum is most scientific selection.
Below by way of specific embodiment, the present invention will be described in detail.It should be appreciated that these embodiments are only exemplary
, it does not constitute a limitation on the scope of protection of the present invention.
Embodiment 1
Administer the cultivated soil that Coast of Guangdong Province soil hardening and heavy metal seriously pollute
1. preparing soil remediation leavening
Step 1: the cultivated soil that soil hardening and heavy metal seriously pollute samples, and screens advantage lactic acid bacteria, specific to screen
Process is as follows:
The configuration of screening and culturing medium: beef extract 10g/L, peptone 10g/L, yeast extract 10g/L, tomato juice 200g/L are spat
Temperature 0.05%, glucose 10g/L, calcium carbonate 15-20g/L, bromocresol green 0.01%, pH 6.0-6.5, agar 10%, high temperature are high
Pressure sterilizing 20min, pours into culture dish and is configured to solid screening and culturing medium.
Inoculation: in the addition 10ml sterile water for the cultivated soil that 1g Coast of Guangdong Province soil hardening and heavy metal are seriously polluted
It is configured to soil suspension, then is made 10 and successively decreases diluted serial suspension, takes dilution 10-4-10-6Each 80- of suspension
100uL is respectively coated in screening and culturing medium, is placed in incubated at room temperature 2-6 days.
Obtain have growth vigor lactic acid bacteria: lactic acid bacteria grow up to after on culture medium displaing yellow, later can thin out black,
It marks the lactic acid bacteria grown at first and 4-6 days bacterium colonies of culture is maximum and the optimal lactic acid bacteria of rising trend is the cream with growth vigor
Sour bacterium, obtains 14 plants of advantage lactic acid bacteria, and conservation is used to prepare leavening use.
Step 2: preparing fermentation raw materials, and according to the following formulation and its component ratio configures: 20 grams of calcium lime powder, brown sugar 20
Gram, 50 grams of edible salt, 30 grams of sodium bicarbonate, 50 grams of ruderal ash, 50 grams of sawdust ash, 0.2 gram of vanadium pentoxide, rice straw powder 50
Gram, add suitable sterile water to stir and evenly mix.
Step 3: being configured to the total 30mL of bacterium solution for 14 plants of advantage lactic acid bacteria for above-mentioned screening obtains, mix with above-mentioned raw materials,
Leavening is made.
2. preparing soil remediation microbial inoculum
Step 1: by raw material and the leavening prepared by following component proportional arrangement: rice straw powder 60.5%, wood dust
15%, ruderal powder 24%, leavening 0.5%.
Step 2: taking leavening according to the above ratio, and leavening is dissolved in the water；Raw material is taken in mentioned component ratio；It will
The leavening being dissolved in the water pours into raw material and is mixed evenly, and is placed on the sealed fermenting room that constant temperature and humidity anaerobism is protected from light
It inside ferments, at 35-37 DEG C, relative humidity control ferments 18 days in 80-90%, is prepared into soil remediation bacterium for temperature control
Agent.
3. administering soil
Step 1: according to Coast of Guangdong Province soil hardening to be administered compared with the cultivated soil that heavy metal seriously pollutes is hardened
Serious situation, by weight, soil remediation microbial inoculum account for 80%, and plant ash accounts for 20% mixing.
Step 2: the soil remediation microbial inoculum that step 1 mixes uniformly is applied on the surface layer of soil to be administered, is simply turned over
Ground site preparation, is uniformly distributed it in the soil.
Step 3: without lying fallow, directly sowing, and makes the advantage lactic acid bacteria in soil remediation microbial inoculum a large amount of numerous in the soil
It grows, voluntarily " improves " hardened and contaminated soil；Soil can plant the vegetables such as cucumber by after microorganism " improvement ".
4. administering result
There is no spray spraying pesticide, uses compound fertilizer and chemical fertilizer, cucumber seedlings and rattan, Ye Yi by the original decrement of amount of application per acre
Straight growing way is vigorous, and without pest and disease damage, blooming, more, knot melon is more, melon and fruit head is larger, and yield is high, improves and produces than general vegetable plot cucumber
30% or more amount, it is in good taste；After two months, observation analysis soil, hardened releasing；Soil is chemically examined, pesticide residue is decomposed completely to be turned
Change, zero residual, each heavy metal species decomposition and inversion reduces 80% or more.
Embodiment 2
Administer the hardened cultivated soil seriously polluted with heavy metal of Soils of Hebei
1. preparing soil remediation leavening
Step 1: the cultivated soil that soil hardening and heavy metal seriously pollute samples, and screens advantage lactic acid bacteria, screening technique
It is identical with embodiment 1,21 plants are filtered out in the cultivated soil that the coastal soil hardening in north to be administered and heavy metal seriously pollute
Advantage lactic acid bacteria.
Step 2: preparing fermentation raw materials, and according to the following formulation and its component ratio configures: 15 grams of calcium lime powder, brown sugar 18
Gram, 53 grams of edible salt, 23 grams of sodium bicarbonate, 48 grams of ruderal ash, 55 grams of sawdust ash, 0.3 gram of vanadium pentoxide, rice straw powder 55
Gram, add suitable sterile water to stir and evenly mix.
Step 3: being configured to the total 30mL of bacterium solution for 21 plants of advantage lactic acid bacteria for above-mentioned screening obtains, mix with above-mentioned raw materials,
Leavening is made.
2. preparing soil remediation microbial inoculum
Step 1: by raw material and the leavening prepared by following component proportional arrangement: rice straw powder 56.5%, wood dust
14%, ruderal powder 28%, leavening 1.5%.
Step 2: taking leavening according to the above ratio, and leavening is dissolved in the water；Raw material is taken in mentioned component ratio；It will
The leavening being dissolved in the water pours into raw material and is mixed evenly, and is placed on the sealed fermenting room that constant temperature and humidity anaerobism is protected from light
It inside ferments, at 35-37 DEG C, relative humidity control ferments 12 days in 80-90%, is prepared into soil remediation bacterium for temperature control
Agent.
3. administering soil
Step 1: according to the hardened hardened especially severe of cultivated soil seriously polluted with heavy metal of Soils of Hebei to be administered
The case where, by weight, soil remediation microbial inoculum accounts for 90%, and plant ash accounts for 10% mixing.
Step 2: randomly selecting the soil hardening and tested with the 0.07 hectare that tills the land that heavy metal seriously pollutes, will
The soil remediation microbial inoculum that step 1 mixes uniformly applies on the surface layer of the selected 0.07 hectare, site preparation of simply ploughing, and makes it
Even distribution is in the soil.
Step 3: without lying fallow, winter wheat can directly be sowed.
4. administering result
Wheat is from being unearthed wheat seeding to maturation, and growing way is vigorous always, and without spray spraying pesticide, there is no insect pests, does not fall
Phenomenon is lied prostrate, irrigation amount is irrigated and subtracts 20%, other compound fertilizers and chemical fertilizer harvest wheat by 40% application of long-term usage amount decrement
650 kilograms, 650 kilograms of per mu yield；The wheat yield in 2015 Hebei, one 480 kilograms of demonstrative project average yield per mu；Another demonstration
580 kilograms of project average yield per mu, 620 kilograms of highest per mu yield；This sample plot wheat is in leading by per mu yield.
Wheat midwifery growth process and soil, hardened releasing are observed after obtaining；Chemical examination soil, the complete decomposition and inversion of pesticide residue,
Zero residual, each heavy metal species decomposition and inversion reduce 80% or more.
Embodiment 3
Without screening advantage lactic acid bacteria step, the hardened cultivated soil seriously polluted with heavy metal of Soils of Hebei is directly taken
Leavening is prepared, remaining condition is all identical with embodiment 2, observes wheat from being unearthed maturation, growing way is vigorous always, does not spray
Pesticide, there is no insect pests, and do not lodge phenomenon, irrigate irrigation amount and subtract 20%, other compound fertilizers and chemical fertilizer are by use throughout the year
40% application of amount decrement, harvests 630 kilograms of wheat, 630 kilograms of per mu yield.Wheat midwifery growth process and soil is observed after obtaining, it is hardened
It releases；Soil, the complete decomposition and inversion of pesticide residue, zero residual are chemically examined, each heavy metal species decomposition and inversion reduces 80% or more.
Use above specific case is illustrated the present invention, is merely used to help understand the present invention, not to limit
The system present invention.For those skilled in the art, according to the thought of the present invention, can also make several simple
It deduces, deform or replaces.
Claims (10)
1. a kind of soil remediation leavening, which is characterized in that the ingredient including following parts by weight: 15-25 parts of calcium lime powder, brown sugar
15-25 parts, 40-60 parts of edible salt, 20-40 parts of sodium bicarbonate, 40-60 parts of ruderal ash, 40-60 parts of sawdust ash, five oxidations two
0.1-0.4 parts of vanadium, 40-60 parts of rice straw powder, soil 1-60 parts to be administered.
2. soil remediation leavening as described in claim 1, which is characterized in that the ingredient including following parts by weight: quick lime
18-23 parts of powder, 18-23 parts of brown sugar, 45-55 parts of edible salt, 25-35 parts of sodium bicarbonate, 45-55 parts of ruderal ash, sawdust ash 45-
55 parts, 0.2-0.3 parts of vanadic anhydride, 45-55 parts of rice straw powder, soil 1-30 parts to be administered.
3. soil remediation leavening as claimed in claim 1 or 2, which is characterized in that the soil to be processed be it is described to
The bacterium solution of the dominant strain screened in processing soil.
4. a kind of method for preparing soil remediation leavening of any of claims 1-3, which is characterized in that by formula
Ingredients Weight ratio weighs the following raw material: 15-25 parts of calcium lime powder, 15-25 parts of brown sugar, and 40-60 parts of edible salt, sodium bicarbonate 20-
40 parts, 40-60 parts of ruderal ash, 40-60 parts of sawdust ash, 0.1-0.4 parts of vanadic anhydride, 40-60 parts of rice straw powder；Then again
Soil 1-60 parts of mixing to be processed are taken, soil remediation leavening is made.
5. a kind of soil remediation microbial inoculum, which is characterized in that including the described in any item leavenings of claim 1-4, by weight,
Ingredient including following ratio is protected from light fermentation through constant temperature and humidity anaerobism and obtains: rice straw powder 55%-65%, wood dust ash 10%-
20%, ruderal ash 20%-30%, the leavening 0.1%-2%.
6. soil remediation microbial inoculum as claimed in claim 5, which is characterized in that by weight, the ingredient including following ratio: rice
Grass meal 60.5%, wood dust ash 15%, ruderal ash 24%, the leavening 0.5%.
7. a kind of method for preparing soil remediation microbial inoculum described in claim 5 or 6, which comprises the steps of:
Raw material preparation: configuring raw material: rice straw powder 55%-65%, wood dust ash 10%-20% by following component weight ratio, wild
Raw weeds ash 20%-30%, the leavening 0.1%-2%；
Prepare microbial inoculum: it is first that the leavening of 0.1%-2% is soluble in water, then pour into the above ratio other raw materials be mixed it is mixed
It is even, it is placed in constant temperature and humidity anaerobism and is protected from light fermentation in fermenting cellar, obtain the soil remediation microbial inoculum；
Preferably, fermentation time is 10-30 days.
8. soil described in any one of soil remediation leavening of any of claims 1-3 or claim 5-6
Administer utilization of the microbial inoculum in the soil for administering soil hardening and agriculture chemical heavy metal comprehensive pollution.
9. a kind of method for administering soil, which is characterized in that by weight by microbial inoculum described in any one of claim 5-7
80%-90% and plant ash 10%-20% is mixed, and is applied in upper soll layer to be administered；Then farming soil to be administered is by bacterium
The mixture of agent and plant ash is uniformly distributed in the soil, without lying fallow, can directly be sowed, paddy field 20 days or so, nonirrigated farmland
40-50 days or so can pesticide heavy-metal residual in decomposition and inversion absorption soil, while soil nutrient, activating plate can be enriched
Tie soil, restore soil in original bioactivity, effectively improve crops it is drought-enduring, it is disease-resistant, resistant to lodging, volume increase the effects of.
10. administering the method for soil as claimed in claim 9, which is characterized in that without lying fallow, directly sow.
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