CN109456918A - A kind of bacillus of quick composting organic materials and its application and organic matter decomposing inoculant - Google Patents
A kind of bacillus of quick composting organic materials and its application and organic matter decomposing inoculant Download PDFInfo
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Abstract
The present invention relates to microorganisms technical field, bacillus and its application and the organic matter decomposing inoculant of a kind of quick composting organic materials are disclosed.The present invention isolates one plant of bacillus for having thermophilic ability from rotten stalk surface, deposit number is CGMCC No.15512, when it is used for humus organic material material, it can be quickly ramped up to 70 DEG C or so of high temperature, and the npk nutrient in high efficiency releasing organics material, reach the standard requirements of biological organic fertilizer with the shorter time, improves production efficiency, can be used for the preparation of biological organic fertilizer, the preparation of microbial bacterial agent, the preparation of organic matter decomposing inoculant and the multiple related fieldss of humus organic material material.
Description
Technical field
The present invention relates to microorganisms technical field, more particularly to a kind of gemma bar of quick composting organic materials
Bacterium and its application and organic matter decomposing inoculant.
Background technique
Shandong District is the main corn-growing regions in the whole nation, Jilin, Hebei, Shandong cultivated area account for the 10% of the whole nation
More than, it is in need of immediate treatment that the meeting after harvest corn leaves a large amount of corn stover.Meanwhile Shandong District is also that facilities vegetable plantation is close
Ji Qu, remaining vegetables straw behind the annual garden Qiu Chaba, utilizes the improper waste and environmental pollution that will also result in resource.As
The most common corn stover of northern area, nutrition organic matter rich in and available chemical component, small part are used as poultry
The raw material of animal husbandry feed, but major part still belongs to agricultural waste resource, because of the high feature of its poor water retention property, crude fiber content, certainly
It so banks up and is difficult to fast rotting, cause huge environmental protection pressure.
The demand reduction cooked with the improvement of living standards, people are used to make a fire to faggot, crop straw burning easily cause
Serious atmosphere polluting problem and using post-process it is improper there is the hardened problem of soil texture, the above processing mode is by political affairs
Mansion department prohibites.In recent years, organic fertilizer is prepared using stalk fermentation, realizes resource circulation utilization, then at waste straw
One of " turning waste into wealth " most effective way.Need to control raw material C/N in organic fertilizer manufacturing process with when moisture content, selection
Efficient decomposing agent, can Rapid Fermentation be decomposed prepares organic fertilizer.And efficiently decomposing agent is selected in organic fertilizer manufacturing process
Difficult point.
The organic fertilizer raw material of northern area, in order to accelerate straw decomposing, shortens fermentation mostly based on stalk and fowl and animal excrement
Period prevents stink diffusion and nutrient loss in fermentation process, and therefore, it is necessary to fully consider the spy of organic fertilizer production raw material
Point could prepare according to property of raw material and apply the preferable decomposing agent of specificity.In the good decomposing agent of specificity can be effective
The function stem of small-molecule substance can be utilized by resolving into plant using organic macromolecules such as protein in raw material, quickly be bred,
The organic matter secreted in the ectoenzyme fast decoupled raw material simultaneously becomes humic acid, N P and K, makes nutrients release.And usually have several
Strain bacterial strain collectively constitutes, and bacterial strain function is complementary to one another, and is that decomposing agent can play a role in the preceding middle and later periods of organic fertilizer manufacturing process.
Currently, the commercially available decomposed temperature of decomposing agent is inadequate, and the decomposed time is longer, it is more difficult to which adaptation is prepared with based on stalk
The bacterial strain of enzyme system complete (cellulase, protease, diastatic activity) is screened in conjunction with local strain resource in the northern market of machine fertilizer, and
The bacterial strain compatibility strong with other function is just able to achieve the efficient decomposed of corn stover.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of bacillus of quick composting organic materials, so that described
Bacillus can be rapidly heated with humus organic material material and reach the requirement of biological organic fertilizer；
Another object of the present invention is that providing above-mentioned bacillus in biological organic fertilizer and microbial bacterial agent etc.
The application of related fields；
Another object of the present invention is that providing the organic matter decomposing inoculant using the preparation of above-mentioned bacillus.
For achieving the above object, the invention provides the following technical scheme:
A kind of bacillus of quick composting organic materials, deposit number are CGMCC No.15512.
The present invention isolates one plant of bacterial strain for having thermophilic ability from rotten stalk surface, is named as J-2, in temperature 40
It is grown rapidly under degrees celsius, reaches logarithmic growth phase at 70 degrees Celsius；The single colonie of the bacterial strain is round, milky, long
It is faint yellow, neat in edge that phase, which cultivates bacterium colony, and opaque, surface flat, edge is complete, sees Fig. 1；
J-2 Gram's staining is purple, for the positive, sees Fig. 2；Gemma takes on a red color in green, thallus in spore staining；Gemma
Bacillus strain is to grow along media surface in aerobic-type test result, is aerobic bacteria.
Using J-2 genome DNA as template, universal primer is that 27F/1492R progress PCR amplification 16s rDNA obtains length
It is that 1.5Kb report is wanted to kiss for highly conserved and consistent length most in the amplified production of 1.6kb, with all bacteriums of 16s rDNA
It closing, sequencing result carries out blast search in the website NCBI, compared with listed 16s rDNA carries out nucleotide homology,
It was found that the 16s rDNA of bacterial strain J-2 and more bacillus category bacterium (Bacillus sp.) similarity highests, download similarity
Higher sequence chooses the highest building systematic evolution tree of 10 plants of similarities, finds bacterial strain J-2 and Bacillus.sp.JN15
(KC121041.1) in the same branch, homology highest (see Fig. 3), in conjunction with cultural characteristic and Physiology and biochemistry qualification result,
Bacillus strain is initially identified as bacillus (Bacillus sp.), and is deposited on March 26th, 2018
China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are CGMCC No.15512.
Bacillus J-2 of the present invention is provided simultaneously with cellulose enzyme activity, proteinase activity and amylase enzyme activity,
And for that can be rapidly heated, temperature reaches as high as 69-73 DEG C, while can be quick in the decomposed fermentation of biological organic fertilizer
Nutrient in releasing organics material reaches the standard of biological organic fertilizer, shortens the production cycle.Based on the beneficial effect, this hair
The bright application for providing the bacillus J-2 in following any one and many aspects:
Biological organic fertilizer is prepared, microbial bacterial agent, humus organic material material is prepared and prepares organic matter decomposing inoculant.
Preferably, the organic materials be one or both of crop material, waste, bacteria residue, sawdust, furfural dregs with
On.Wherein, the stalk includes but is not limited to wheat, rice, corn, potato, rape, cotton, sugarcane stalk, be may be selected any
It is one or more；The waste refers mainly to fowl and animal excrement, including but not limited to cow dung, chicken manure, duck excrement, pig manure, may be selected any
It is one or more；Bacteria residue refers mainly to various edible fungi residues, any one or more may be selected.
According to above-mentioned application, the present invention provides a kind of organic matter decomposing inoculants, including deposit number is CGMCC
The bacillus of No.15512.
Preferably, the bacillus J-2 in the decomposing agent can cooperate multiple microbial strains to form complex microorganism
Decomposing agent, such as bacillus licheniformis, bacillus subtilis, aspergillus niger and Trichoderma viride；
In the specific embodiment of the invention, in parts by weight, the decomposing agent includes 20-45 parts of CGMCC No.15512
Bacillus, 10-35 parts of bacillus licheniformis, 15-20 parts of bacillus subtilises, 10-15 parts of aspergillus nigers and 10-15 parts green
Trichoderma.
In the specific embodiment of the invention, the decomposing agent includes the bacillus of 25 parts of CGMCC No.15512,30
Part bacillus licheniformis, 15 parts of bacillus subtilises, 15 parts of aspergillus nigers and 15 parts of Trichoderma virides；Or including 20 parts of CGMCC
The bacillus of No.15512,25 parts of bacillus licheniformis, 20 parts of bacillus subtilises, 15 parts of aspergillus nigers and 10 parts of green wood
It is mould；Or bacillus, 10 parts of bacillus licheniformis, 20 parts of bacillus subtilises, 10 parts including 45 parts of CGMCC No.15512
Aspergillus niger and 15 parts of Trichoderma virides；
Wherein, the bacillus subtilis is selected from the bacillus subtilis and strain number that strain number is ACCC19373
For one or both of the bacillus subtilis of ACCC19743；The bacillus licheniformis is selected from strain number
One or both of the bacillus licheniformis that the bacillus licheniformis of ACCC19747 and strain number are ACCC02975；It is described
Aspergillus niger is the aspergillus niger that strain number is ACCC32589；The Trichoderma viride is the green wood that strain number is ACCC30206
It is mould；ACCC is the abbreviation of Chinese agriculture Microbiological Culture Collection administrative center in strain number, and each bacterial strain is purchased from this
The heart.
More specifically decomposing agent includes 20 parts of J-2 bacillus, 15 parts of aspergillus niger (ACCC32589), Trichoderma viride
(ACCC30206) 10 parts, 15 parts of bacillus subtilis (ACCC19373), 5 parts of bacillus subtilis (ACCC19743), lichens bud
25 parts of spore bacillus (ACCC19747), 10 parts of bacillus licheniformis (ACCC02975)；Or including 25 parts of J-2 bacillus, black song
15 parts of mould (ACCC32589), 15 parts of Trichoderma viride (ACCC30206), 10 parts of bacillus subtilis (ACCC19373), withered grass bud
5 parts of spore bacillus (ACCC19743), 20 parts of bacillus licheniformis (ACCC19747), 10 parts of bacillus licheniformis (ACCC02975)；
Or including 45 parts of J-2 bacillus, 10 parts of aspergillus niger (ACCC32589), 15 parts of Trichoderma viride (ACCC30206), withered grass gemma
20 parts of bacillus (ACCC19373), 10 parts of bacillus licheniformis (ACCC19747).
Based on the excellent effect of aforementioned bacillus J-2, the decomposing agent has can quickly rise compared with similar products
Mild humus organic material material and the advantage for the requirement for reaching biological organic fertilizer, therefore the present invention proposes the decomposing agent simultaneously and exists
Application in following any one or more aspects:
It prepares biological organic fertilizer, prepare microbial bacterial agent and humus organic material material.
From the above technical scheme, the present invention isolates one plant of gemma bar for having thermophilic ability from rotten stalk surface
Bacterium when being used for humus organic material material, can be rapidly heated to 70 DEG C or so of high temperature, and the nitrogen in high efficiency releasing organics material
Phosphorus potassium nutrition reaches the standard requirements of biological organic fertilizer with the shorter time, improves production efficiency, can be used for biological organic fertilizer
Preparation, the preparation of microbial bacterial agent, the preparation of organic matter decomposing inoculant and the multiple related fieldss of humus organic material material.
Biological deposits explanation
J-2, classification naming: bacillus subtilis, Bacillus subtilis. are deposited in China on March 26th, 2018
Microbiological Culture Collection administration committee common micro-organisms center, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, in
Institute of microbiology, the academy of sciences, state, deposit number are CGMCC No.15512.
Detailed description of the invention
Fig. 1 show J-2 strain morphology；
Fig. 2 show J-2 bacterial strain Gram's staining result；
Fig. 3 show J-2 strain gene group and the high strain sequence of homology compares constructed phyletic evolution tree graph.
Specific embodiment
The invention discloses a kind of bacillus of quick composting organic materials and its application and organic matter decomposing inoculants, originally
Field technical staff can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that all similar
Replace and change apparent to those skilled in the art, they are considered as being included in the present invention.Institute of the present invention
It states bacillus and its application and decomposing agent is described by preferred embodiment, related personnel can obviously not depart from
Bacillus described herein and its application and decomposing agent are modified or are suitably changed in the content of present invention, spirit and scope with
Combination, carrys out implementation and application the technology of the present invention.
Microbial strains in decomposing agent of the present invention usually require to be activated and expanded culture, and specific method can
Referring to as follows:
1, bacillus J-2 strain is prepared
Bacillus is grown in LB liquid medium, and cultivation temperature is at 30-37 DEG C, in 150~170r/ of revolving speed
Min shakes shaking flask culture, and incubation time is 18-24h, then sample detection OD650 value and the LB liquid medium for not connecing strain
Comparison；
Bacillus culture to dilution 5~6 times after OD value be 0.2~0.6 when, by Bacillus solution according to
5~10% volume ratio is added in solid medium, and cultivation temperature is 28-37 DEG C, cultivates 7~12d, then 40 DEG C or less bakings
It is dry, gemma bacillus agent is obtained by crushing dry or natural drying.
The LB liquid medium is made of 8~10g of peptone, 3~5g of yeast powder, 8~10g of sodium chloride.pH 7.0
~7.5, said components are settled to 1000mL with distilled water, sterilize 20-30min at being 120-121 DEG C in temperature.
The seed concentration of bacillus is 8 × 108~12 × 108cfu/mL.The formula of solid medium by wheat bran 70~
80kg, 8~10kg of dregs of beans, 6~8kg of corn flour, 0.2~0.3kg of ammonium sulfate, 0.8~1.1kg of glucose, potassium dihydrogen phosphate 0.2
~0.4kg, 0.02~0.05kg of ferrous sulfate, 0.02~0.05kg of iron magnesium sulfate, 0.02~0.05kg of calcium chloride, tap water
100~120kg composition.The effective clump count of the gemma bacillus agent prepared in this way is 1.5~2 × 1010Cfu/g, water content
15~20%.
2, Bacillus subtilis strain is prepared
The bacillus subtilis original seed in -80 DEG C of preservations of temperature is taken, is forwarded on LB liquid medium, in temperature 30-37
Constant temperature activation culture for 24 hours, is transferred to liquid fermentation tank in strain logarithmic growth phase and expands culture or the expansion of solid state fermentation bed at DEG C
It cultivates, 28-37 DEG C of cultivation temperature, cultivation cycle 1-6d, fermenting microbe is by crushing under the conditions of 40-60 DEG C of obtained drop temperature
Drying is to water content 20% hereinafter, obtaining the bacillus subtilis microbial agent of fineness >=70%, 13,000,000,000 cfu/g or more；
3, Bacillus licheniformis strain is prepared
The bacillus licheniformis original seed in -80 DEG C of preservations of temperature is taken, is forwarded on LB liquid medium, in temperature 30-37
Constant temperature activation culture for 24 hours, is transferred to liquid fermentation tank in strain logarithmic growth phase and expands culture or the expansion of solid state fermentation bed at DEG C
It cultivates, 28-35 DEG C of cultivation temperature, cultivation cycle 1-5d, fermenting microbe is by crushing under the conditions of 40-60 DEG C of obtained drop temperature
Drying is to water content 20% hereinafter, obtaining the bacillus licheniformis agent of fineness >=70%, 10,000,000,000 cfu/g or more；
4, Trichoderma viride strain is prepared
Take trichoderma original seed, be forwarded in liquid PDA culture medium, at 30-35 DEG C of temperature constant temperature activation culture for 24 hours, in bacterium
Kind logarithmic growth phase is transferred to liquid fermentation tank and expands culture or the expansion culture of solid state fermentation bed, 28-35 DEG C of cultivation temperature, cultivates
Period 6-15d, under the conditions of 40-60 DEG C of obtained drop temperature fermenting microbe through crushing it is dry to water content 20% hereinafter,
To fineness >=70%, the Trichoderma viride microbial inoculum of 2,000,000,000 cfu/g or more；
It is mixed after each strain preparation according to decomposing agent ratio of the present invention, the living bacteria count > 500,000,000 of organic matter decomposing inoculant
Cfu/g, fineness >=70%, decomposing agent moisture content≤20%, pH 5.5-8.5, cellulase activity >=200U/ml, protease activity
>=200U/g/ml, diastatic activity >=10U/g/ml.
The dosage of the decomposing agent is 0.1%-the 1% of organic materials compost fermentation total weight.
Below with reference to embodiment, the present invention is further explained.
The Enzyme activity assay of embodiment 1:J-2 bacterial strain
By bacterial strain switching in LB liquid medium, 30 DEG C~37 DEG C, 120~150rpm cultivates 3~4d, until thallus is broken
It is broken, bacteria suspension is drawn at this time and carries out Enzyme activity assay, and the neutral cellulase under the conditions of cellulase activity is 60 DEG C is living, protease activity
For the neutral protease under the conditions of 40 DEG C, diastatic activity is the neutral starch enzyme activity under the conditions of 60 DEG C.
Cellulase activity is detected to make substrate with sanlose (CMC), and CMC is generated after cellulose hydrolyzation
Reduced sugar, 3,5- dinitrosalicylic acids (DNS) are a kind of oxidants, can act on reduced sugar, nitro is made to be reduced into amino, solution
Become orange, orange depth is directly proportional to concentration of reduced sugar.Therefore, the content of reduced sugar, Jin Ercong are acquired using colorimetric method
The quantity of reduced sugar acquires the size of cellulase activity.1mL original sample bacteria suspension, 1min generate 1 microgram glucose and are defined as 1
Enzyme activity unit (U).Specific detection method is referring to NY609-2002.
Protease hydrolyzed casein substrate under the conditions of certain temperature and pH generates the amino acid containing phenolic group (such as: junket
Propylhomoserin, tryptophan etc.), under alkaline condition, Folin reagent (Folin) is restored, molybdenum blue and tungsten blue is generated, uses spectrophotometry
Measurement, calculates its enzyme activity.1mL bacteria suspension, under certain condition, it is 1 enzyme activity that 1min hydrolyzed casein, which generates 1 μ g tyrosine,
Unit (U).When proteinase activity detects, need for bacteria suspension to be diluted 3~4 times of processing, reaction product needs 9000~
After 12000rpm is centrifuged 3~5min, color development treatment is carried out, specific detection method is referring to NY609-2002.
Soluble starch is amylopectin, and amylase can be cut into short chain dextrin different in size, a small amount of maltose
And glucose, and starch is made to fade away iodine rufous specific reaction, the speed that color disappears is related with enzymatic activity, therefore
Its enzyme activity can be calculated by the absorbance after fixed reaction.1mL liquid bacteria suspension is under conditions of 60 DEG C, pH=6.0,1min
Liquefy 1mg soluble starch, and as 1 enzyme activity unit is indicated with u/mL.Reaction product needs 9000~12000rpm centrifugation 3
After~5min, color development treatment is carried out, specific detection method is referring to NY609-2002.
Table 1
| Index | J-2 |
| Cellulase activity (U/mL) | 30.46 |
| Protease activity (U/mL) | 17.05 |
| Diastatic activity (U/mL) | 3.45 |
As shown in Table 1, bacillus J-2 cellulase activity of the present invention is greater than 30U/mL, and protease activity is greater than 15U/mL, forms sediment
Powder enzyme activity is greater than 3U/mL；Effective 500,000,000 cfu/g of clump count > of the gemma bacillus agent is prepared using solid fermentation.
Embodiment 2: decomposing agent of the present invention
The organic matter decomposing inoculant based on J-2 bacillus is prepared, is matched according to weight ratio as follows: J-2 withered grass gemma
20 parts of bacillus, 15 parts of aspergillus niger (ACCC32589), 10 parts of Trichoderma viride (ACCC30206), bacillus subtilis
(ACCC19373) 15 parts, 5 parts of bacillus subtilis (ACCC19743), 25 parts of bacillus licheniformis (ACCC19747), lichens bud
10 parts of spore bacillus (ACCC02975).
5.05 hundred million cfu/g of living bacteria count of the organic matter decomposing inoculant, fineness 70%, moisture content 15.18%, pH
Value is 5.57.It is packed by filling liquid and solid bacterium powder form, packing specification 20kg, liquid form packing specification 20L.
Embodiment 3: decomposing agent of the present invention
The organic matter decomposing inoculant based on J-2 bacillus is prepared, is matched according to weight ratio as follows: J-2 withered grass gemma
25 parts of bacillus, 15 parts of aspergillus niger (ACCC32589), 15 parts of Trichoderma viride (ACCC30206), bacillus subtilis
(ACCC19373) 10 parts, 5 parts of bacillus subtilis (ACCC19743), 20 parts of bacillus licheniformis (ACCC19747), lichens bud
10 parts of spore bacillus (ACCC02975)；
10.55 hundred million cfu/g of living bacteria count of the organic matter decomposing inoculant, fineness are more than or equal to 70%, moisture content
13.15%, pH value 5.78.It is packed by filling liquid and solid bacterium powder form, packing specification 20kg, liquid form packet
Fill specification 50L.
Embodiment 4: decomposing agent of the present invention
The organic matter decomposing inoculant based on J-2 bacillus is prepared, is matched according to weight ratio as follows: J-2 withered grass gemma
45 parts of bacillus, 10 parts of aspergillus niger (ACCC32589), 15 parts of Trichoderma viride (ACCC30206), bacillus subtilis
(ACCC19373) 20 parts, 10 parts of bacillus licheniformis (ACCC19747).
32.75 hundred million cfu/g of living bacteria count of the organic matter decomposing inoculant, fineness are more than or equal to 70%, moisture content
11.59%, pH value 7.85.It is packed by filling liquid and solid bacterium powder form, packing specification 20kg, liquid form packet
Fill specification 50L.
Embodiment 5: organic material composting test
Test site: Shandong Province, Boxing County, Accessories during Binzhou；
Experimental condition: under the conditions of 5 DEG C of original ambient temperature, with fresh cow dung, corn stover, mushroom dreg in production vehicle
Aerobic composting fermentation production biological organic fertilizer is aerated in indirect fermentation pond.
3 groups of repetitions are arranged in 4 processing of test setting, each processing；
Processing 1: J-2 strain is removed on the basis of 2 decomposing agent of embodiment, original J-2 strain number is assigned to withered grass gemma
On bacillus (ACCC19373) and bacillus subtilis (ACCC19743), the two is 20 parts；
Handle 2: hundred favour straw decomposing inoculants；
Processing 3: 2 decomposing agent of embodiment；
Processing 4: blank control.
Corn stover is crushed to 3~5cm length using crusher, for cow dung using cow dung is not dehydrated, bacteria residue is mushroom bacteria
Slag, by potassium dichromate method and Kai Shi nitriding respectively in raw material organic matter and total nitrogen content detect, by decomposed original
C/N control is expected in 35:1-40:1, and in mass, by calculating, stalk dosage, cow dung dosage, mushroom dreg amount ratio are 2:
The raw material mixed is divided into four heaps by 0.9:0.1, every heap 500kg, control material moisture in 45%-60%, inoculum concentration according to
2.5kg decomposing agent is added in the 0.5% of weight, and fermentation megathermal period temperature reaches 55-75 DEG C of progress turning and throwing once, fermentation maturity time
15 days.
Within the fermentation maturity period, reach in temperature carry out turning over throwing within the scope of 55-70 DEG C respectively, throwing 5-7 times is turned over, at 5
Npk nutrient content is measured by sampling in sampled point, and detection method the results are shown in Table 2 referring to NY525-2012.
Table 2
As can be seen from Table 2, under identical fermentation condition and environment, the total nutrient of decomposing agent of the present invention meets life
Organic fertilizer standard NY884-2012 reaches 5% or more, and other processing fermentations 15 days still below standard；Meanwhile it is of the present invention
Temperature of the decomposing agent in decomposed period reaches as high as 70 DEG C, facilitates the quick composting of organic materials, and other handle highest temperatures
Degree is 62 DEG C.
Embodiment 6: organic material composting test
Test site: Shandong Province, Boxing County, Accessories during Binzhou；
Experimental condition: under the conditions of 15 DEG C of original ambient temperature, with fresh cow dung, corn stover in workshop fermentation vat
Inside it is aerated aerobic composting fermentation production biological organic fertilizer.
Processing 1: J-2 strain is removed on the basis of 4 decomposing agent of embodiment, original J-2 strain number is assigned to withered grass gemma
On bacillus (ACCC19373) and bacillus licheniformis (ACCC19747), the two is respectively 40 parts and 35 parts；
Handle 2: hundred favour straw decomposing inoculants；
Processing 3: 4 decomposing agent of embodiment；
Processing 4: blank control.
Corn stover is crushed to 3~5cm length using crusher, cow dung passes through potassium bichromate using cow dung is not dehydrated
Method and Kai Shi nitriding respectively in raw material organic matter and total nitrogen content detect, decomposed raw material C/N is controlled in 35:1-
40:1, in mass, by calculating, stalk dosage, cow dung amount ratio are 2:1, and the raw material mixed is divided into four heaps, every heap
500kg, control material moisture are added 2.5kg decomposing agent according to the 0.5% of weight, ferment the megathermal period in 45%-60%, inoculum concentration
Temperature reaches 55-75 DEG C of progress turning and throwing once, and the fermentation maturity time 15 days.
Within the fermentation maturity period, reach in temperature carry out turning over throwing within the scope of 55-70 DEG C respectively, throwing 5-7 times is turned over, at 5
Npk nutrient content is measured by sampling in sampled point, and detection method the results are shown in Table 3 referring to NY525-2012 and NY884-2012.
Table 3
As can be seen from Table 3, under identical fermentation condition and environment, the total nutrient of decomposing agent of the present invention meets life
Organic fertilizer standard NY884-2012 reaches 5% or more, and other processing fermentations 15 days still below standard；Meanwhile it is of the present invention
Temperature of the decomposing agent in decomposed period reaches as high as 69 DEG C, facilitates the quick composting of organic materials, and other handle highest temperatures
Degree is 64 DEG C.
Embodiment 7: organic material composting test
Test site: Shandong Province, Boxing County, Accessories during Binzhou；
Experimental condition: under the conditions of 27 DEG C of original ambient temperature, with fresh pig manure, corn stover, sawdust in workshop
Aerobic composting fermentation production biological organic fertilizer is aerated in fermentation vat.
Processing 1: J-2 strain is removed on the basis of 3 decomposing agent of embodiment, original J-2 strain number is assigned to withered grass gemma
On bacillus (ACCC19373) and bacillus subtilis (ACCC19743), the two is 20 parts；
Handle 2: hundred favour straw decomposing inoculants；
Processing 3: 3 decomposing agent of embodiment；
Processing 4: blank control.
Corn stover is crushed to 3~5cm length using crusher, using pig manure is not dehydrated, sawdust use does not take off pig manure
Water sawdust, by potassium dichromate method and Kai Shi nitriding respectively in raw material organic matter and total nitrogen content detect, will be rotten
Ripe raw material C/N control is in 35:1-40:1, and in mass, by calculating, stalk dosage, pig manure dosage, sawdust amount ratio are 2:
The raw material mixed is divided into four heaps by 0.8:0.2, every heap 500kg, control material moisture in 45%-60%, inoculum concentration according to
2.5kg decomposing agent is added in the 0.5% of weight, and fermentation megathermal period temperature reaches 55-75 DEG C of progress turning and throwing once, fermentation maturity time
15 days.
Within the fermentation maturity period, reach in temperature carry out turning over throwing within the scope of 55-70 DEG C respectively, throwing 5-7 times is turned over, at 5
Npk nutrient content is measured by sampling in sampled point, and detection method the results are shown in Table 4 referring to NY525-2012 and NY884-2012.
Table 4
As can be seen from Table 4, under identical fermentation condition and environment, the total nutrient of decomposing agent of the present invention meets life
Organic fertilizer standard NY884-2012 reaches 5% or more, and other processing fermentations 15 days still below standard；Meanwhile it is of the present invention
Temperature of the decomposing agent in decomposed period reaches as high as 73 DEG C, facilitates the quick composting of organic materials, and other handle highest temperatures
Degree is 65 DEG C.
Embodiment 8: decomposed temperature comparisons
Processing 1: J-2 strain is removed on the basis of 3 decomposing agent of embodiment, original J-2 strain number is assigned to withered grass gemma
On bacillus (ACCC19373) and bacillus subtilis (ACCC19743), the two is 20 parts；
Handle 2: hundred favour straw decomposing inoculants；
Processing 3: 3 decomposing agent of embodiment；
Processing 4: blank control.
Corn stover is crushed to 3~5cm length using crusher, is divided into four heaps, every heap 100kg dry weight, inoculum concentration is pressed
0.5kg decomposing agent is added according to the 0.5% of weight, fermentation megathermal period temperature reaches 55-75 DEG C of progress turning and throwing once, unites in 5 points
Meter temperature is averaged, and the results are shown in Table 5.
Table 5
| Time (moon day) | Handle 1 (DEG C) | Handle 2 (DEG C) | Handle 3 (DEG C) | Handle 4 (DEG C) |
| 10.14 | 54 | 58 | 46 | 40 |
| 10.16 | 58 | 65 | 61 | 56 |
| 10.17 | 63 | 63 | 61 | 60 |
| 10.18 | 65 | 60 | 70 | 60 |
| 10.19 | 68 | 65 | 68 | 66 |
| 10.20 | 66 | 65 | 65 | 64 |
| 10.21 | 60 | 61 | 62 | 50 |
As can be seen from Table 5, decomposing agent of the present invention faster heats up than other processing, and maximum temperature also highest, this is to having
The decomposed fermentation of machine material is advantageous.
Embodiment 9: greenhouse straw decomposing inoculant application test
In June, 2017 carries out in Accessories during Binzhou Boxing County Shandong Bo Hua Efficient Eco-agriculture Science and Technology Ltd. greenhouse
Field trial.1.2 mu of the effective cultivated area of the canopy.In soil category heavy salinizedly, soil pH > 8.25, salt content > 5g/
Kg carries out soil ecology improvement after pulling out garden this year June, and specific embodiment is to crush the garden stalk of pulling out on the spot in canopy,
By 15 side's cow dungs/canopy amount, cow dung semi-decomposed is sprinkling upon soil surface, then repaves crushing maize stalk in cow dung top,
Vegetables straw-cow dung-corn stover three-decker is formed, 20~25cm of thickness uniformly spreads fertilizer over the fields decomposing agent, and flood irrigates, and
Surface covers one layer of mulch, reduces moisture evaporation and weed growth.Whole process keeps ventilation in canopy, observes stalk after 1 month
Variation detects the soil organism and viable count variation and the variation of periodic logging ground temperature.
2 processing are arranged in this test: processing 1: spreading fertilizer over the fields the decomposing agent in 20kg embodiment 2 per acre, the decomposing agent is by J-
2 gemma bacillus agents, the solid bacteriums dosage such as bacillus licheniformis, Trichoderma viride, aspergillus niger are mixed, after mixing, are spread
Apply stalk surface；Processing 2: control greenhouse is used after 20kg decomposing agent high-temperature inactivation by the method in processing 1.
Separately sampled detection soil organic matter content, viable count changes of contents after (autumn stubble), processing before treatment, and periodically
Track ground temperature variation and stalk rotten level in canopy.Soil root system variation is as shown in table 6.
Table 6
There is apparent variation afterwards before treatment in the soil organism and viable count, wherein handling the increase of 1 soil organism
Increase obviously compared with processing 2, while the soil organism increases, has also driven the original existing mushroom breeding of soil, handled 1 work
Bacterium number is higher than processing 2, and in entire ecological improved, process, ground temperature is maintained between 35~45 DEG C, variation shadow of the temperature by weather
Sound is larger.After ecology improvement, corn stover organic matter also drops to 50% or so, and the stalk that can not will rot completely screws in ground,
It carries out secondary decomposed.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (11)
1. a kind of bacillus of quick composting organic materials, which is characterized in that deposit number is CGMCC No.15512.
2. application of the bacillus that deposit number is CGMCC No.15512 in following any one and many aspects:
Biological organic fertilizer is prepared, microbial bacterial agent, humus organic material material is prepared and prepares organic matter decomposing inoculant.
3. applying according to claim 2, which is characterized in that the organic materials be crop material, waste, bacteria residue, sawdust,
One or more of furfural dregs.
4. a kind of organic matter decomposing inoculant, which is characterized in that the bacillus for being CGMCC No.15512 including deposit number.
5. decomposing agent according to claim 4, which is characterized in that the gemma bar for being CGMCC No.15512 including deposit number
Bacterium, bacillus licheniformis, bacillus subtilis, aspergillus niger and Trichoderma viride.
6. decomposing agent according to claim 4, which is characterized in that in parts by weight, including 20-45 parts of CGMCC No.15512
Bacillus, 10-35 parts of bacillus licheniformis, 15-20 parts of bacillus subtilises, 10-15 parts of aspergillus nigers and 10-15 parts green
Trichoderma.
7. according to the decomposing agent of claim 5 or 6, which is characterized in that the bacillus subtilis is selected from strain number
One or both of the bacillus subtilis that the bacillus subtilis of ACCC19373 and strain number are ACCC19743.
8. according to the decomposing agent of claim 5 or 6, which is characterized in that the bacillus licheniformis is selected from strain number
One or both of the bacillus licheniformis that the bacillus licheniformis of ACCC19747 and strain number are ACCC02975.
9. according to the decomposing agent of claim 5 or 6, which is characterized in that the aspergillus niger is that strain number is ACCC32589's
Aspergillus niger.
10. according to the decomposing agent of claim 5 or 6, which is characterized in that the Trichoderma viride is that strain number is
The Trichoderma viride of ACCC30206.
11. application of the decomposing agent described in claim 5-11 any one in following any one or more aspects:
It prepares biological organic fertilizer, prepare microbial bacterial agent and humus organic material material.
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