CN103980010A - Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant - Google Patents

Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant Download PDF

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CN103980010A
CN103980010A CN201410242443.1A CN201410242443A CN103980010A CN 103980010 A CN103980010 A CN 103980010A CN 201410242443 A CN201410242443 A CN 201410242443A CN 103980010 A CN103980010 A CN 103980010A
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livestock
poultry
feces
heap
temperature
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CN103980010B (en
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何宗均
梁海恬
赵琳娜
田阳
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Tianlan Diwo (Tianjin) Agricultural Technology Co.,Ltd.
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TIANJIN AGRICULTURAL RESOURCE AND ENVIRONMENT INST
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.

Description

A kind of feces of livestock and poultry decomposing microbial inoculum and using method and made biological organic fertilizer
Technical field
The invention belongs to agriculture microorganism in the Application Areas of processing on feces of livestock and poultry, relate to a kind of feces of livestock and poultry decomposing microbial inoculum and using method thereof and made biological organic fertilizer.
Background technology
At present, China's Amount of poultry excrements has reached 18.84 hundred million t, be equivalent to 3.4 times of trade waste quantity discharged, 10 years from now on, livestock and poultry breeding industry is also by the speed increase with 8%, feces of livestock and poultry is not processed and applied because major part obtains, the direct or indirect negative impact water body, atmosphere, soil, HUMAN HEALTH and the ecosystem having been caused, dealing with the environmental problem that feces of livestock and poultry causes, is current problem anxious to be resolved.Application feces of livestock and poultry decomposing microbial inoculum fast processing feces of livestock and poultry is produced organic fertilizer, is the Main overcoming the above problems both at home and abroad, and this technology is at home and abroad widely used.
At present, domestic feces of livestock and poultry decomposing microbial inoculum has often only related to the capacity of decomposition of Mierocrystalline cellulose and protein in feces of livestock and poultry, function is often more single, microbial inoculum itself and extension product organic fertilizer performance are single, added value is not high, income in agricultural can not get improving naturally, thereby affects the smooth solution of above problem.
Based on this, special proposition the present invention.This invention considers the singularity of feces of livestock and poultry itself and the functional performance of extension product organic fertilizer, as the deodoriging properties of decomposing microbial inoculum, and disease-resistant performance and the bacterial manure performance etc. of decomposing microbial inoculum and extension product organic fertilizer thereof.
Summary of the invention
The object of this invention is to provide a kind of being specifically designed to and process decomposing microbial inoculum and the using method thereof of feces of livestock and poultry, and with the bio-organic fertilizer of this microbial inoculum and special process fermentative production.
A kind of feces of livestock and poultry decomposing microbial inoculum provided by the invention, it is characterized in that it is by can decomposition of cellulose, protein, deodorizing, disease-resistant and multiple bacterial strain bacterial manure several functions is composited, its activeconstituents comprises subtilis (Bacillus subtilis), streptomyces microflavus (Streptomyces microflavus), long handle wood mould (Trichoderma longbrachiatum) and Bacillus mucilaginosus (Bacillus mucilaginosus), it is made up of each single strain fermented product of following portions by weight: 0.5～2 part of subtilis, 0.5～2 part of streptomyces microflavus, 0.5～2 part of long handle wood mould 0.5～2 part and Bacillus mucilaginosus, poultry manure decomposing agent living bacteria count is 6 × 10 8individual/g.
It is the bacterial strain of ACCC 11089 that described subtilis specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.It is the bacterial strain of ACCC 40027 that described streptomyces microflavus specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.Described long handle wood is mould, and specifically to can be Chinese agriculture microbial strains preservation administrative center deposit number be the bacterial strain of ACCC 30150.It is the bacterial strain of ACCC 10013 that described Bacillus mucilaginosus specifically can be Chinese agriculture microbial strains preservation administrative center deposit number.
Described feces of livestock and poultry decomposing microbial inoculum has several functions, comprises and can carry out fermentation maturity and deodorizing to feces of livestock and poultry, can anti-cucumber fusarium axysporum and be applied to farm crop and can increase production.Described decomposing microbial inoculum can be used for the quick composting of the feces of livestock and poultry such as ox, pig, chicken, sheep, horse, duck, and can add in right amount other organic materials, as brown coal etc.
The present invention further discloses the using method of feces of livestock and poultry decomposing microbial inoculum, it is characterized in that being undertaken by following step:
1, adjust the carbon-nitrogen ratio (25～30:1) of feces of livestock and poultry and moisture (55～60%, with hand one hold hand seam drip be advisable), every 5 side's feces of livestock and poultry add 1 kilogram of composite fungus agent, turn over and smash evenly.
2, by trapezoidal feces of livestock and poultry heap growth good pre-treatment, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, and heap is long to be determined according to place, and every heap volume is no less than 5 sides.
3, after fermentation starts, in the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once.If fermentation initial stage temperature does not rise, adjust moisture to recommendation ratio and turn over every day and smash once until pile temperature rising.
4, when heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once;
5, turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, and repeat this step, until heap temperature is identical with external temperature, moisture is while being down to below 30%, complete fermenting process, whole fermentation period is generally 15～25 days.
The present invention further discloses poultry manure decomposing microbial inoculum livestock manure fermented become thoroughly decomposed and deodorizing on application, and application in arviculture volume increase, is wherein applied to mu volume increase 17.4% in wild cabbage plantation.
The invention also discloses a kind of bio-organic fertilizer, it is characterized in that it is to be become thoroughly decomposed and formed by described feces of livestock and poultry decomposing microbial inoculum fermented livestock ight soil, wherein every 5 side's feces of livestock and poultry add 1 kilogram of feces of livestock and poultry decomposing microbial inoculum, and the parts by weight of described feces of livestock and poultry are as follows: 40～60 parts of chicken manures; 20～40 parts of cow dungs; 5～15 parts of pig manures; 5～15 parts, brown coal.
Described chicken manure organic content >=45%, total nitrogen content >=2.5%; Cow dung organic content >=50%; Pig manure organic content >=45%; Lignite humic acid content >=50%.
The present invention further discloses the production method of bio-organic fertilizer, it is characterized in that being undertaken by following step:
1, the mixing of raw material: fully mix taking by weight chicken manure, cow dung, pig manure and brown coal mixer;
2, add water: add water by the weight ratio of water and mixing raw material 1:1, control water content 50～60%;
3, add feces of livestock and poultry decomposing agent: every 5 side's feces of livestock and poultry add 1 kilogram of composite fungus agent;
4, the specification of banking up: will regulate water and add the feces of livestock and poultry heap of composite fungus agent to grow up trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, heap is long to be determined according to place, every heap volume is no less than 5 sides;
5, the control of turning time: keep three days in the time that 20～30 centimetres of depths temperature of charge rise to 55 DEG C, then turn over and smash once, when heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost;
6, turning requires: turn in buttress process, to accomplish to adjust, even, broken, adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section, to reach the object of abundant fermentation; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work;
7, adding of object bacteria: in the fermentation later stage, when fermentation starts to lower the temperature, add Bacillus mucilaginosus single strain microbial inoculum, every square object material add-on is 1kg;
8, fermentation termination control: heap temperature is identical with external temperature, moisture is while being down to below 30%, has completed the whole fermentation production process of biological organic fertilizer, and the whole cycle is generally 15～20 days, 25～30 days time in winter;
9, fermented quality control: pulverize after having fermented, sieve, detect, weigh and pack, bio-organic fertilizer products production completes, the organic requirement of bio-organic fertilizer reaches 45%～55%, N, P, K total nutrient 5%, living bacteria count > 0.2 × 10 8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
The present invention further discloses the application of biological organic fertilizer aspect vegetable disease-resistant effect of increasing production.Described biological organic fertilizer is applied in shallot plantation has obvious disease-resistant yield-increasing effect, and preventive effect reaches 56%, and volume increase reaches 20.5%.
Decomposing microbial inoculum energy quick composting feces of livestock and poultry provided by the invention, there is good deodoriging properties, and then output high-quality can be disease-resistant bio-organic fertilizer, the disadvantage such as can avoid because feces of livestock and poultry becomes thoroughly decomposed that thorough do not brought disease and pest is high, seedling rate is low, burns seedling, dead seedling, weeds are many.Can significantly increase the content of the nutritive elements such as the soil organism and phosphorus, potassium by the become thoroughly decomposed bio-organic fertilizer product produced of described decomposing microbial inoculum, improve quantity of useful microbe in Nutrient Transformation rate and product, promote soil remediation, culture fertility, can reduce fertilizer application amount, strengthens the disease resistance of crop simultaneously, improve crop quality, reduce and drop into, improve output, promote to increase income.
Brief description of the drawings:
Fig. 1 is preparation technology's schema of bio-organic fertilizer.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is ordinary method.Test materials used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment, results averaged for three times.
Following bacterial strain is all available from Chinese agriculture microbial strains preservation administrative center (Agricultural Culture Collection of China is called for short ACCC):
Subtilis (Bacillus subtilis): ACCC deposit number is 11089, is called for short ACCC11089;
Streptomyces microflavus (Streptomyces microflavus): ACCC deposit number is 40027, is called for short ACCC 40027;
Long handle wood mould (Trichoderma longbrachiatum): ACCC deposit number is 30150, is called for short ACCC30150;
Bacillus mucilaginosus (Bacillus mucilaginosus): ACCC deposit number is 10013, is called for short ACCC10013.
embodiment 1:
the preparation of substratum and bacterial classification
One, the preparation of substratum
1, potato, glucose agar medium (PDA)
200 grams of potatos removing skin, are cut into small pieces, and the 1000ml that adds water boils 30 minutes, elimination potato ball, filtrate is complemented to 1000ml, add 20 grams of glucose, 15 grams, agar, dissolves rear packing, 15 pounds of sterilizings 30 minutes, pH nature, does not add agar when obtaining liq substratum.
2, nutrition nutrient agar
Formed by peptone 5g, sodium-chlor 5g, extractum carnis 3g and distilled water 1000ml.PH7.0(solid medium adds agar 15-20g).
3, synthetic No. 1 substratum of Gao Shi
KNO 31g, K 2hPO 40.5g, NaCl 0.5g, FeSO 47H 2o 0.01g, MgSO 47H 2o 0.5g Zulkovsky starch 20g, pH7.2～7.4, distilled water 1000ml.
4, potassium bacteria culture medium
Yeast extract paste 0.4g, N.F,USP MANNITOL 10.0g, K 2hPO 40.5g, MgSO 47H 2o 0.2g, FeCl 30.005g, CaCO 31.0g, distilled water 1000.0ml, PH7.0-7.2, add 15 grams of agar when preparation solid medium.
5, solid fermentation substratum
Wheat bran 80g, dregs of beans 10g, (NH 4) 2sO 41.0g, KH 2pO 40.2g, MgSO 47H 200.05g, 80mL distilled water.
Two, the activation of bacterial classification
Subtilis bacterium is seeded on nutrition nutrient agar substratum; Mould being seeded on PDA substratum of long handle wood; Streptomyces microflavus is seeded on synthetic No. 1 substratum of Gao Shi; Bacillus mucilaginosus is seeded on potassium bacteria culture medium
Mould to subtilis, streptomyces microflavus, long handle wood and Bacillus mucilaginosus are carried out respectively to inclined-plane switching, access respective ramp substratum, activation culture 3-7 days.
Three, single bacterial classification preparation
1, the cultivation of subtilis and fermentation
Slant medium and shake-flask culture base are all nutrition nutrient agar, when preparation solid medium, add 15 grams of agar, and seeding tank and fermentation tank culture medium are soya-bean milk, the starch of 6.5kg, the 1kg glucose of 1000kg water, 8.75kg soybean, CaCO 3100g, FeCl 310g, pH7.0,, cultivates 24～36 hours at 28 DEG C～30 DEG C, when bacterium number in every ml fermented liquid reaches 10 × 10 after 30 minutes through 121 DEG C of real tank sterilizings 8individual/g.Then being adsorbed on the vermiculite of 1 part normal temperature by the fermented liquid of 3 parts of weight ratios respectively dries and obtains subtilis single strain microbial inoculum.
2, the cultivation of Bacillus mucilaginosus and fermentation
Slant medium and shake-flask culture base are all potassium bacteria culture medium, and seeding tank and fermentation tank culture medium are soya-bean milk, the starch of 6.5kg, the 1kg glucose of 1000kg water, 8.75kg soybean, CaCO 3100g, FeCl 310g, pH7.0,, cultivates 48 hours at 28 DEG C～30 DEG C, when bacterium number in every ml fermented liquid reaches 5 × 10 after 30 minutes through 121 DEG C of real tank sterilizings 8individual/g.Then being adsorbed on the vermiculite of 1 part normal temperature by the fermented liquid of 3 parts of weight ratios respectively dries and obtains Bacillus mucilaginosus single strain microbial inoculum.
3, mould cultivation and the fermentation of long handle wood
Inclined-plane and shake-flask culture base are potato, glucose agar medium, and slant medium adds agar.Liquid nutrient medium is sub-packed in 500ml triangular flask after sterilizing, accesses each fungi slant strains, shaking table speed 180r/min, cultivates 48 hours for 28 DEG C～30 DEG C, and when occurring in shaking flask that density is large, mycelium pellet is obviously not muddy.Seeding tank and fermentation cylinder for fermentation substratum are the cooking liquor of starch, 1kg glucose and the 2kg potato of soya-bean milk, the 6.5kg of 1000kg water, 8.75kg soybean, pH7.0, through 121 DEG C of real tank sterilizings after 30 minutes, cultivate 48 hours at 28 DEG C～30 DEG C, after mycelium pellet that density is large appears in fermented liquid, be inoculated on solid fermentation substratum, 10%, 121 DEG C of inoculum size, 0.1MPa autoclaving 30min, 25 DEG C of solid fermentations are cultivated 48h, and spore count reaches 6 × 10 8individual/g.
4, the cultivation of streptomyces microflavus and fermentation
Inclined-plane and shake-flask culture base are synthetic No. 1 substratum of Gao Shi, and slant medium adds agar.Liquid nutrient medium is sub-packed in 500ml triangular flask after sterilizing, accesses each fungi slant strains, shaking table speed 180r/min, cultivates 5～7 days for 28 DEG C～30 DEG C, and when occurring in shaking flask that density is large, mycelium pellet is obviously not muddy.Seeding tank and fermentation cylinder for fermentation substratum are the cooking liquor of starch, 1kg glucose and the 2kg potato of soya-bean milk, the 6.5kg of 1000kg water, 8.75kg soybean, pH7.0, through 121 DEG C of real tank sterilizings after 30 minutes, cultivate 48 hours at 28 DEG C～30 DEG C, after mycelium pellet that density is large appears in fermented liquid, be inoculated on solid fermentation substratum, 10%, 121 DEG C of inoculum size, 0.1MPa autoclaving 30min, 25 DEG C of solid fermentations are cultivated 5～7 days, and spore count reaches 6 × 10 8individual/g.
Four, the preparation of decomposing microbial inoculum (representing with composite fungus agent below)
Mould and the Bacillus mucilaginosus list bacterial classification weight ratio of the subtilis of producing as stated above, streptomyces microflavus, long handle wood is 0.5～2:0.5～2:0.5～2:0.5～2.Mixing makes composite fungus agent after mixing thoroughly, and composite fungus agent living bacteria count reaches 6 × 10 8individual/g.
 
embodiment 2:
composite fungus agent composition
A kind of feces of livestock and poultry decomposing microbial inoculum, is formulated as follows (by weight):
The composition of table 1 feces of livestock and poultry decomposing microbial inoculum
Title Subtilis Streptomyces microflavus Long handle wood is mould Bacillus mucilaginosus
（1） 0.5 2.0 1.0 2.0
（2） 1.0 1.5 2.0 1.5
（3） 1.5 1.0 1.5 1.0
（4） 2.0 0.5 0.5 0.5
Wherein living bacteria count is 6 × 10 8individual/g;
The proportioning (percentage ratio by weight) of table 2 bio-organic fertilizer raw materials for production
Title Chicken manure (%) Cow dung (%) Pig manure (%) Brown coal (%)
（1） 40 40 10 10
（2） 45 30 15 10
（3） 50 30 5 15
（4） 60 20 15 5
embodiment 3:
mutual antagonistic experiment between bacterial strain
Mould to subtilis, streptomyces microflavus after activation, long handle wood and Bacillus mucilaginosus are carried out to the mutual antagonistic experiment between bacterial strain, and combination of two, is divided into into 12 kinds of array modes, three repetitions of each combination.
Adopt steel ring method to carry out bacteriostatic test, bacterial strain is cultivated in corresponding substratum to 3-7 days (190r/min, 28 DEG C) and made fermented liquid.Getting the wherein fermented liquid 0.1mL of a kind of bacterium (A) is applied on PDA flat board, then on the solid medium of each flat board, put the steel ring of two prior sterilizings, in each steel ring, add again the fermented liquid of any bacterium (B) outside the A bacterium of 0.25mL, each processing in triplicate, taking the plate that only connects A bacterium as contrast.Cultivate and the antibacterial situation of routine observation for 28 DEG C.The results are shown in Table 3.
Antagonistic effect between table 3 bacterial classification
? Subtilis Streptomyces microflavus Long handle wood is mould Bacillus mucilaginosus
Subtilis ? - - -
Streptomyces microflavus - ? - -
Long handle wood is mould - - ? -
Bacillus mucilaginosus - - - ?
Note: "-" represents antagonism reaction negative, "+" represents antagonism reacting positive.
Test-results shows unrestraint effect between the mould and 4 kinds of bacterium of Bacillus mucilaginosus of subtilis, streptomyces microflavus, long handle wood, and antagonism reaction is all negative.
embodiment 4:
the mensuration of the Mierocrystalline cellulose of single bacterial strain and composite fungus agent and protein capacity of decomposition
One, cellulase activity measuring method
1, reagent and solution
Substrate solution: accurately take 0.625 g sodium carboxymethyl-cellulose, be dissolved in 100.0 mL sodium phosphate buffers (0.2 M, pH 6.0), heated and stirred makes it to dissolve.
DNS developer: take 10.0 g 3,5-dinitrosalicylic acid is dissolved in distilled water, add 20.0 g sodium hydroxide, 200.0 g Seignette salts and 500.0 ml water, after heating for dissolving, add again re-distilled phenol 2.0 g, sodium sulphite anhydrous 99.3 0.5 g cooling after all dissolving, be dissolved to 1000.0 mL.
2, plant and instrument
Spectrophotometer, electronic balance, vibrator incubator.
3, Specification Curve of Increasing
Standard glucose solution: dissolve 25.0mg glucose with distilled water and be settled to 25.0mL.
Get 5 test tubes with 20.0 ml scales, measure reagent by upper table.In every pipe, add 1.0 ml 2.0 M sodium hydroxide solutions and 2.0 ml DNS nitrite ions, shake up to be placed in boiling water bath and accurately heat after 5.0 min, flowing water is cooling, distilled water is settled to 20.0 ml, after shaking up, measure the OD value of each pipe in 490 nm places, taking the μ g number of glucose as X-coordinate, OD value is ordinate zou, drawing standard curve.
Table 4 Specification Curve of Increasing table
Test tube number Standard glucose solution (ml) Sodium phosphate buffer (0.2M pH 6.0) (ml) In test tube, (μ g) for glucose amount
1 0 5 0
2 0.4 4.6 400
3 0.7 4.2 800
4 1.6 3.4 1600
5 3.2 1.8 3200
4, former state enzyme liquid
Take 10 times of 5.0g sample distilled water dilutings, fully vibration, static 30min, filters, and its filtrate is former state enzyme liquid.
5, determination step
Get 3 test tubes with 20.0ml scale, 1 as blank, and all the other 2 as parallel sample pipe.In sample hose, add 1.0ml former state enzyme liquid, then in 3 test tubes, add respectively 4.0ml to be preheated to the substrate solution of 60 DEG C, in the water-bath of 60 DEG C, reacting 20min takes out, every pipe adds 2.0M sodium hydroxide solution and the 2.0ml DNS nitrite ion of 1.0ml immediately, adds 1.0ml former state enzyme liquid after shaking up in control tube again.3 test tubes are put into boiling water bath, after colour developing 5min, take out immediately, flowing water is cooling, is dissolved to 20.0ml, surveys its OD value with spectrophotometer in 490nm place.
6, cellulase activity calculates:
Be converted into glucose μ g number, U=(M according to typical curve 1-M 0)/20
In formula: U: the enzyme activity (μ g/min) of sample; M 0: (μ is g) for contrast glucose amount
M 1: after decomposing, (μ is g) for example weight; 20: the enzyme-to-substrate reaction times (min)
1ml former state enzyme liquid, per minute produces 1 μ g glucose and is defined as 1 enzyme activity unit (u).
Two, proteinase activity measuring method
1, reagent: according to the regulation in QB/T 1803.
2, the preparation of former state enzyme liquid
Take sample 10.0g(or 10.0ml), add in the triangular flask (150ml) that granulated glass sphere is housed, then add the phosphoric acid buffer (pH7.5) of certain volume to dissolve, 200 r/min shaking table vibration 30min, then four layers of filtered through gauze, filtrate is again in the centrifugal 20min of 3000r/min.Supernatant liquor after centrifugal is diluted to proper concn according to damping fluid for enzyme activity (pH7.5), for test.
3, determination step: other just has two places to change according to the regulation of QB/T1803 in concrete mensuration: the one, the reaction times (adding after casein) changes 30min into by 10min; The 2nd, former static filtration changes centrifugal 5min into and filters.
Three, enzyme assay result
To prepare that subtilis, streptomyces microflavus, long handle wood are mould, Bacillus mucilaginosus and composite fungus agent carry out cellulase and protease activity determination as stated above, measurement result sees the following form.
Table 5 enzymic activity situation
Process Cellulase activity (u/ml) Protease activity (u/ml)
Subtilis 68.00 47.20
Streptomyces microflavus 12.51 -
Long handle wood is mould 95.08 15.74
Bacillus mucilaginosus 15.64 8.94
Composite fungus agent 60.08 21.37
Detected result shows, there is very strong Mierocrystalline cellulose and protein capacity of decomposition by subtilis, streptomyces microflavus, long handle wood composite fungus agent mould, that 4 kinds of bacterial strains of Bacillus mucilaginosus form, higher than the requirement of cellulase activity 30 (u/ml) and the protease activity 15 (u/ml) of organic matter decomposing inoculant national Specification, composite fungus agent can be used as organic matter decomposing inoculant and uses.
embodiment 5:
the fermentative deodorizing ability of single bacterial strain and composite fungus agent is measured
One, agents useful for same and fermented substrate
1, sulphuric acid soln: sulfuric acid 0.005mol, is settled to 1L with distilled water.
2, starch indicating liquid: 1g starch adding distil water 10ml, injects the distilled water that 200ml boils, micro-2min that boils.
3, tetrabromo-mcresolsulfonphthalein ethanolic soln: 0.10g tetrabromo-mcresolsulfonphthalein, is settled to 100ml with ethanol.
4, methyl red-tetrabromo-mcresolsulfonphthalein indicator: 0.1% tetrabromo-mcresolsulfonphthalein ethanolic soln of 3 volumes mixes with 0.2% methyl red ethanolic soln of 1 volume.
5, acetic acid zinc solution: take 50g zinc acetate and be dissolved in a small amount of distilled water, add 2 Glacial acetic acid, stir to solution and become limpid, add 2 ethanol, be settled to 1L with distilled water.
6, methyl red ethanolic soln preparation: 0.20g methyl red, is settled to 100ml with ethanol.
7, boric acid solution: 20.41g boric acid and 1000ml distilled water.
8, iodine standard solution: 13g iodine and 35g potassiumiodide are dissolved in 100mL distilled water, is settled to 1000mL with distilled water, shakes up and is stored in brown bottle.
9, hypo solution: 13g five water Sulfothiorine and 0.5g sodium carbonate, be settled to 1L with distilled water, slowly boils 10min, is contained in brown bottle.
10, hydrochloric acid (1+1) solution: by 1 volume water and 1 volume 37% hydrochloric acid.
11, fermentative deodorizing matrix: be made up of dried poultrymanure 60g, straw powder 10g and water, water content is 50%.
Two, measuring method
The main component of the foul smell that compost produces is ammonia and hydrogen sulfide, very large to people and animals' harm, and in embodiment, main ammonia detection, sulfurated hydrogen detection and the organoleptic detection of adopting evaluated the deodorizing capability of microbiobacterial agent.
1, ammonia detection method
With the ammonia in 2% boric acid solution absorption gas, then use the titration of 0.005mol/L sulphuric acid soln, when titration, first dripping methyl red-tetrabromo-mcresolsulfonphthalein mixture indicator 5-6 drips in having absorbed the boric acid solution of ammonia, use 0.005mol/L sulphuric acid soln titration again until the pink terminal that arrives calculates ammonia level according to the consumption of sulphuric acid soln (volume).
Reaction equation is: (1) NH 3+ H 3bO 3→ NH 4h 2bO 3; (2) NH 4h 2bO 3+ H 2sO 4→ (NH 4) 2sO 4+ 2H 3bO 3.
The calculation formula of the amount of substance of ammonia: n 1=0.005*(V 1-V 01) * 10 -3; n 1the amount of substance (mol) of-ammonia, the amount of substance concentration (mol/L) of 0.005-sulfuric acid, V 1the consumption of sulphuric acid soln (ml) when the titration of-test solution, V 01the consumption of sulphuric acid soln (ml) when-blank titration.
2, sulfurated hydrogen detection method
Iodimetry,iodometry is to absorb the hydrogen sulfide in unstripped gas with zinc acetate, adds excess iodine standard titration solution S in slightly acidic solution 2-be oxidized to simple substance S, remaining iodine sodium thiosulfate solution titrated, according to hypo solution consumption, calculates hydrogen sulfide content; 5mL iodine standard solution, 5mL hydrochloric acid (1+1) solution are added in the 50g/L zinc acetate solution that has absorbed hydrogen sulfide, confined reaction 5 minutes, use 0.05mol/L sodium thiosulfate solution titrated, when nearly terminal, drip 0.5% Starch Indicator, to becoming colorless, record the volume of hypo solution used, can calculate the content of hydrogen sulfide.
The reaction formula of iodometric titrationiodimetry titration: (1) H 2s+Zn 2+→ ZnS ↓+2H +; (2) ZnS+I 2→ Zn 2++ 2I -+ S ↓; (3) I 2+ 2S 2o 3 2-→ 2I -+ S 4o 6 2-.
The calculation formula of the amount of substance of hydrogen sulfide: n 2=[0.5-0.05*(V 02-V 2)/2] * 10 -3; n 2the amount of substance (mol) of-hydrogen sulfide, the amount of substance (mol) of 0.5-standard iodine solution, the amount of substance concentration (mol/L) of 0.05-hypo solution, V 02the consumption of hypo solution (ml) when-blank titration, V 2the consumption of hypo solution (ml) when the titration of-test solution.
3, experimental technique
Subtilis, Bacillus licheniformis, sporotrichum thermophile, aspergillus oryzae and viride after activation are cultivated 2 days with liquid nutrient medium respectively, obtained the bacterium liquid of each bacterial strain.
Bacterium liquid and the composite fungus agent of each bacterial strain are tested respectively as follows: bacterium liquid is connected in fermentative deodorizing matrix, in 500mL beaker, mix (every part of fermentative deodorizing matrix inoculation 10mL bacterium liquid), as experiment sample, fermentative deodorizing matrix sample is in contrast set, kraft paper and the sealing of one deck plastic paper for each sample.Each sample (six kinds of experiment samples and a kind of check sample) arranges three parallel processing, and each parallel processing arranges three repetitions, and result is got the mean value of three repetitions.The ammonia of the small beaker of 2% boric acid solution of containing 20mL in first parallel middle placement in order to produce in absorbing fermenting process, measures with acidimetry on the 7th day; The hydrogen sulfide of the 50g/L acetic acid zinc solution of containing 20mL in second parallel middle placement in order to produce in absorbing fermenting process, the 7th day with iodometric determination; The 3rd parallel, within the 7th day, carries out sense organ mensuration.
4, experimental result
The results are shown in Table 6.
Table 6 fermentative deodorizing ability
Process Foul smell grade Ammonia level (10 -3mol） Hydrogen sulfide content (10 -3mol）
Subtilis + 0.00260 0.32321
Streptomyces microflavus + 0.00250 0.32509
Long handle wood is mould +++ 0.00325 0.43461
Bacillus mucilaginosus ++ 0.00310 0.40098
Composite fungus agent + 0.00220 0.30765
Contrast ++++ 0.00713 0.65432
Note: how many expression sense organs of "+" are measured foul smell grade, "+" more multilist shows that sense organ measures more smelly.
Detected result in conjunction with organoleptic detection and ammonia and hydrogen sulfide content shows, by subtilis, streptomyces microflavus, long handle wood is mould and Bacillus mucilaginosus forms composite fungus agent deodorizing effect significantly better than single bacterial strain and contrast.
 
embodiment 6:
the resistance against diseases of single bacterial strain and composite fungus agent is measured
Adopt steel ring method to carry out bacteriostatic test, mould to subtilis, streptomyces microflavus, long handle wood after activation, Bacillus mucilaginosus and composite fungus agent are carried out to bacteriostatic test with cucumber fusarium axysporum respectively, three repetitions of each combination.
Each bacterial strain is cultivated in corresponding substratum to 2-3 days (190r/min, 28 DEG C) and made fermented liquid, it is 10 that cultured cucumber fusarium axysporum is made to spore concentration 6the bacteria suspension of individual/mL.Getting cucumber fusarium axysporum bacteria suspension 0.1mL is applied on PDA flat board, then on the solid medium of each flat board, put the steel ring of two prior sterilizings, in each steel ring, add again above-mentioned each fermented liquid and the composite bacteria liquid of 0.25mL, each processing in triplicate, taking the plate that only connects cucumber fusarium axysporum bacteria suspension as contrast.Cultivate and the antibacterial situation of routine observation for 28 DEG C.
The each bacterial strain of table 7 and the bacteriostasis of composite fungus agent to cucumber fusarium axysporum
Note: "+" represents antagonism reacting positive, and "+" more multilist shows that inhibition is larger.
Test-results shows, subtilis, streptomyces microflavus, long handle wood are mould, Bacillus mucilaginosus and composite fungus agent all have fungistatic effect to cucumber fusarium axysporum, and wherein composite fungus agent is the strongest.
embodiment 7:
the effect of increasing production of single bacterial strain and composite fungus agent
1, test site and varieties of plant
Test vegetables are wild cabbage.
2, field design
Totally 6 processing, 3 repetitions of each processing, conventional fertilizer application, community area 96m 2.
Process 1: subtilis; Process 2: streptomyces microflavus; Process 3: long handle wood is mould; Process 4: Bacillus mucilaginosus; Process 5: composite fungus agent; Process 6: contrast.
3, test-results
The each bacterial strain of table 8 and composite fungus agent field effect of increasing production
Process Community mean yield (kg) Per mu yield (kg) Volume increase (%)
Process 1 480 3335 14.3
Process 2 482 3349 14.8
Process 3 478 3321 13.8
Process 4 490 3404 16.7
Process 5 495 3425 17.4
Process 6 420 2918 -
Test-results shows, subtilis, streptomyces microflavus, long handle wood are mould, Bacillus mucilaginosus and composite fungus agent all have effect of increasing production than contrast in wild cabbage plantation, wherein composite fungus agent the best, better than single bacterial strain, can be used as bacterial manure and use in farmland.
 
embodiment 8:
the using method of composite fungus agent and effect
By above embodiment, illustrate by subtilis, streptomyces microflavus, long handle wood is mould, 4 kinds of bacterium of Bacillus mucilaginosus form composite fungus agent, there is the effect of several functions, can be used as the quick composting that organic matter decomposing inoculant carries out feces of livestock and poultry, can be used as the deodorizing that reodorant carries out feces of livestock and poultry, can be used as the control that disease-resistant microbial inoculum carries out crop pest, can also be applied to as agricultural microorganism microbial inoculum the cultivation of farm crop, play the effect of volume increase.
One, the using method of composite fungus agent
1, adjust the carbon-nitrogen ratio (25～30:1) of feces of livestock and poultry and moisture (55～60%, with hand one hold hand seam drip be advisable), every 5 side's feces of livestock and poultry add 1 kilogram of composite fungus agent, turn over and smash evenly.
2, by trapezoidal feces of livestock and poultry heap growth good pre-treatment, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, and heap is long to be determined according to place, and every heap volume is no less than 5 sides.
3, after fermentation starts, in the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once.If fermentation initial stage temperature does not rise, adjust moisture to recommendation ratio and turn over every day and smash once until pile temperature rising.
4, when heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once;
5, turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, and repeat this step, until heap temperature is identical with external temperature, moisture is while being down to below 30%, complete fermenting process, whole fermentation period is generally 15～25 days.
Two, the result of use of composite fungus agent
1, materials and methods
Composting material: chicken manure, cow dung, pig manure and brown coal.Weight ratio is that chicken manure accounts for 40～60%; Cow dung accounts for 20～40%; Pig manure 5～15%; Brown coal 5～15%.C/N ratio is 37.
If two processing, process: add described invention feces of livestock and poultry decomposing microbial inoculum; Contrast: with the blank of microbial inoculum, each processing 2T raw material, repeats for three times, averages, buttress type fermentation reactor system.Fermentation period calculates gained till being down to room temperature with leavening temperature, ferment and two processing were carried out to sampling analysis in the 16th day, comprise available N, P, K total amount (%), C/N ratio, bacterium number and intestinal bacteria, average, and the physical condition of observing fermentation, result is as shown in the table:
2, results and analysis
Table 9 composite fungus agent becomes thoroughly decomposed and deodorizing effect
As shown in Table 9, from indices, process ferment effect and be better than contrast, wherein process leavening temperature and in 2 days, can reach 55 DEG C, contrast needs 5 days; The Bearing performance of fermentation is active for processing microbe population growth and breeding, and bacterium number and available N, P, K content are as shown in table 5 all higher than contrast, and fermentation period also shortens greatly, wherein be treated to 16 days, contrast 28 days, process fermentation color chocolate, odorless, and contrast is light color, frowziness.The aftertreatment in 16 days of fermenting is respectively 18.6,23.0 with contrast C/N ratio, and C/N is lower than, and microorganism is better to the effect of feces of livestock and poultry cellulose degradation.Therefore,, from bacterium number, available nutrient, C/N ratio, smell and fermentation period effect, illustrate and add described composite fungus agent all to play facilitation effect to becoming thoroughly decomposed of feces of livestock and poultry with deodorizing.
embodiment 9:
application composite fungus agent is produced made biological organic fertilizer and field effect
Under certain site facility condition, by adding decomposing microbial inoculum, feces of livestock and poultry generation biological degradation, chemical degradation and physical change, produce CO 2, H 2o and NH 3deng gas release of heat, generate in a large number and can be transformed and absorb micromolecular inorganic and organic nutrient substance by crop, form stable physical structure and state, obtain bio-organic fertilizer product of the present invention.
One, the production of biological organic fertilizer and technique
1, raw material and formula: biological organic fertilizer raw materials for production comprise chicken manure, cow dung, pig manure and four kinds of organic materialss of brown coal.By weight, chicken manure accounts for 40～60%; Cow dung accounts for 20～40%; Pig manure 5～15%; Brown coal 5～15%.
2, material choice: chicken manure organic content >=45%, total nitrogen content >=2.5%; Cow dung organic content >=50%; Pig manure organic content >=45%; Lignite humic acid content >=50%.
3, proportioning raw materials: take by weight 40～60% chicken manure, 20～40% cow dung, 5～15% pig manure and 5～15% brown coal.
4, the mixing of raw material: the dried poultrymanure taking by weight, dry cow dung, dry pig manure and brown coal are fully mixed with mixer.
5, add water: by water: the weight ratio of mixing raw material 1:1 adds water, control water content in 50～60% left and right.
6, add composite fungus agent of the present invention: every 5 side's feces of livestock and poultry add 1 kilogram of composite fungus agent
7, the specification of banking up: will regulate water and add the feces of livestock and poultry heap of composite fungus agent to grow up trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, heap is long to be determined according to place, every heap volume is no less than 5 sides.
8, the control of turning time: after the fermentation of banking up starts, if fermentation initial stage temperature does not rise, as inadequate in moisture content, adjust moisture to recommendation ratio 50～60%, as excessive in moisture content, must turn over every day and smash once, increase oxygen content in buttress, impel microorganism Fast Growth, until heap temperature rises.In the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once, in order to killing harmful pathogenic micro-organism and the worm's ovums such as excrement enterobacteria and roundworm egg.When heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once.Turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost.
9, turning requires: turn in buttress process and will accomplish tune, even, broken.Adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section, to reach the object of abundant fermentation; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work.
10, adding of object bacteria: in the fermentation later stage, when fermentation starts to lower the temperature, add Bacillus mucilaginosus single strain microbial inoculum, every square object material add-on is 1kg.
11, fermentation termination control: heap temperature is identical with external temperature, moisture is while being down to below 30%, outward appearance chocolate, odorless, has completed the whole fermentation production process of biological organic fertilizer, and the whole cycle is generally 15～20 days, 25～30 days time in winter.
12, fermented quality control: pulverize after having fermented, sieve, detect, weigh and pack, bio-organic fertilizer products production completes.The organic requirement of bio-organic fertilizer reaches 45%～55%, N, P, K total nutrient 5%, living bacteria count > 0.2 × 10 8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
Because described composite fungus agent integrates cellulose decomposition, protein decomposition, deodorizing, disease-resistant and bacterial manure several functions, add after feces of livestock and poultry material, probiotics decomposes on the one hand the feces of livestock and poultry that becomes thoroughly decomposed and forms stable biological organic fertilizer, utilize feces of livestock and poultry to carry out self expanding propagation on the one hand, own disease-resistant and bacterial manure function transplanting in the biological organic fertilizer generating, make the biological organic fertilizer generating there is equally the multiple performances such as disease-resistant and bacterial manure, see technical process Fig. 1.
Two, using method
Use 200～500 kilograms as base manure with every mu, before ploughing, spread fertilizer over the fields and earth's surface, then following ploughs is evenly manured into soil and interiorly uses as base manure; When ditch spread, in field of vegetables, trench digging is sprinkled into fertilizer, then moves seedling or dibbling, mu 200 kilograms of left and right of consumption; Dibbling or while transplanting, can take the method for spreading manuer in holes, and after fertilizer and fine earth can being mixed, puts into every cave, and then transplanting or dibbling, mu 10 kilograms of left and right of consumption.
Three, field effect
Trial crops is shallot, and soil situation is available nitrogen 73PPm, rapid available phosphorus 33PPm, available potassium 165PPm, organic matter 1.85%, total salt 0.117%, pH7.89, community area 40m 2, test area is 0.36 mu, 10 mu of demonstration areas.
Test design: establish two processing: 1, conventional fertilizer application+biological organic fertilizer, 2, conventional fertilizer application (contrast); Repeat for three times, order is arranged.Test-results is as shown in table 10:
Table 10 biological organic fertilizer field effect
Test-results shows, biological organic fertilizer of the present invention has obvious disease-resistant yield-increasing effect, and preventive effect reaches 56%, and volume increase reaches 20.5%.

Claims (10)

1. a feces of livestock and poultry decomposing microbial inoculum, it is characterized in that it is by can decomposition of cellulose, protein, deodorizing, disease-resistant and multiple bacterial strain bacterial manure several functions is composited, its activeconstituents comprises subtilis (Bacillus subtilis), streptomyces microflavus (Streptomyces microflavus), long handle wood mould (Trichoderma longbrachiatum) and Bacillus mucilaginosus (Bacillus mucilaginosus), it is made up of each single strain fermented product of following portions by weight: 0.5～2 part of subtilis, 0.5～2 part of streptomyces microflavus, 0.5～2 part of long handle wood mould 0.5～2 part and Bacillus mucilaginosus, poultry manure decomposing agent living bacteria count is 6 × 10 8individual/g.
2. the using method of feces of livestock and poultry decomposing microbial inoculum described in claim 1, is characterized in that being undertaken by following step:
(1) adjust the carbon-nitrogen ratio (25～30:1) of feces of livestock and poultry and moisture (55～60%, with hand one hold hand seam drip be advisable), every 5 side's feces of livestock and poultry add 1 kilogram of described decomposing microbial inoculum, turn over and smash evenly;
(2) by trapezoidal feces of livestock and poultry heap growth good pre-treatment, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, and heap is long to be determined according to place, and every heap volume is no less than 5 sides;
(3) after fermentation starts, in the time that temperature of charge (20～30 centimetres of depths) rises to 55 DEG C, keep three days, then turn over and smash once,
If fermentation initial stage temperature does not rise, adjust moisture to recommendation ratio and turn over every day and smash once until pile temperature rising;
(4) when heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once;
(5) turn over and smash according to heap tender feeling condition afterwards, temperature rise is not turned over and is smash period, and temperature stops rising and turns over and smash, and repeat this step, until heap temperature is identical with external temperature, moisture is while being down to below 30%, complete fermenting process, whole fermentation period is generally 15～25 days.
3. described in claim 1, feces of livestock and poultry decomposing microbial inoculum has several functions, comprises and can carry out fermentation maturity and deodorizing to feces of livestock and poultry, can anti-cucumber fusarium axysporum and be applied to farm crop and can increase production, be wherein applied to mu volume increase 17.4 in wild cabbage plantation
%。
Described in claim 1 feces of livestock and poultry decomposing microbial inoculum in the application of the capacity of decomposition of Mierocrystalline cellulose and protein.
Described in claim 1 feces of livestock and poultry decomposing microbial inoculum livestock manure fermented become thoroughly decomposed and deodorizing on application.
6. the application of feces of livestock and poultry decomposing microbial inoculum in arviculture volume increase described in claim 1.
7. a bio-organic fertilizer, it is characterized in that it is to be become thoroughly decomposed and formed by feces of livestock and poultry decomposing microbial inoculum fermented livestock ight soil described in claims 1, wherein every 5 side's feces of livestock and poultry add 1 kilogram of feces of livestock and poultry decomposing microbial inoculum, and the parts by weight of described feces of livestock and poultry are as follows: 40～60 parts of chicken manures; 20～40 parts of cow dungs; 5～15 parts of pig manures; 5～15 parts, brown coal.
8. bio-organic fertilizer claimed in claim 7, wherein chicken manure organic content >=45%, total nitrogen content >=2.5%; Cow dung organic content >=50%; Pig manure organic content >=45%; Lignite humic acid content >=50%.
9. the production method of bio-organic fertilizer described in claim 7, is characterized in that being undertaken by following step:
(1) mixing of raw material: fully mix taking by weight dried poultrymanure, dry cow dung, dry pig manure and brown coal mixer;
(2) add water: add water by the weight ratio of water and mixing raw material 1:1, control water content 50～60%;
(3) add feces of livestock and poultry decomposing agent: every 5 side's feces of livestock and poultry add 1 kilogram of composite fungus agent;
(4) specification of banking up: will regulate water and add the feces of livestock and poultry heap of composite fungus agent to grow up trapezoidal, 1 meter of heap top width, 2 meters of heap bottom width, pile 0.8～1 meter of high summer and autumn, 1.2～1.4 meters of winter-spring season, heap is long to be determined according to place, every heap volume is no less than 5 sides;
(5) control of turning time: keep three days in the time that 20～30 centimetres of depths temperature of charge rise to 55 DEG C, then turn over and smash once, when heap temperature rises to after 55 DEG C again, keep three days, then turn over and smash once, in the time that temperature exceedes 70 DEG C, need turn over and smash immediately, eliminate the inhibition of high temperature to microorganism growth by cooling, thereby impel the activity of a large amount of mesophilic bacterias, accelerate the process of compost;
(6) turning requires: turn in buttress process, to accomplish to adjust, even, broken, adjusting is exactly original top section in buttress to be transferred to earth's surface become underclad portion, and underclad portion is transferred to top layer and is become top section, to reach the object of abundant fermentation; Even is exactly that buttress Raw is mixed to also inhomogeneous part, again mixes work; Broken is exactly that the massive zymolysis thing of formation is during the fermentation carried out to fragmentation work;
(7) adding of object bacteria: in the fermentation later stage, when fermentation starts to lower the temperature, add Bacillus mucilaginosus single strain microbial inoculum, every square object material add-on is 1 kilogram;
(8) fermentation termination control: heap temperature is identical with external temperature, moisture is while being down to below 30%, has completed the whole fermentation production process of biological organic fertilizer, and the whole cycle is generally 15～20 days, 25～30 days time in winter;
(9) fermented quality control: pulverize after having fermented, sieve, detect, weigh and pack, bio-organic fertilizer products production completes, the organic requirement of bio-organic fertilizer reaches 45%～55%, N, P, K total nutrient 5%, living bacteria count > 0.2 × 10 8individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
10. the application of biological organic fertilizer aspect raising vegetable disease-resistant effect of increasing production described in claim 7.
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CN105154373A (en) * 2015-10-08 2015-12-16 山东大学 High-temperature cow dung decomposition agent and preparation method thereof
CN106083460A (en) * 2016-06-13 2016-11-09 镇江贝思特有机活性肥料有限公司 A kind of bio-feritlizer preventing and treating watermelon blight
CN105948946A (en) * 2016-06-29 2016-09-21 固镇县华丰蔬菜专业合作社 Microbial fertilizer for preventing green Chinese onions from rust disease
CN108485995A (en) * 2018-03-19 2018-09-04 辽宁省农业科学院 A kind of complex micro organism fungicide and biological organic fertilizer promoting hickory chick growth
CN108299033A (en) * 2018-04-17 2018-07-20 福建圣德生物科技有限公司 A kind of biological organic fertilizer and its preparation method
CN109824393A (en) * 2019-04-09 2019-05-31 河北省农林科学院农业资源环境研究所 A kind of compost method reducing ammonia emission in animal waste fermentation process
CN110105152A (en) * 2019-05-16 2019-08-09 宿迁润沃生物科技有限公司 A method of organic fertilizer is made using feces of livestock and poultry
CN112852666A (en) * 2021-01-19 2021-05-28 新疆河润水业有限责任公司 Preparation method of microbial agent and microbial fertilizer prepared by adopting microbial agent
CN114561329A (en) * 2022-04-21 2022-05-31 中国农业科学院农业资源与农业区划研究所 Compound microbial inoculum for fermenting livestock and poultry manure and fermentation method thereof

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