CN103980010B - Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant - Google Patents
Livestock and poultry manure decomposing inoculant, use method thereof and biological organic fertilizer prepared by using livestock and poultry manure decomposing inoculant Download PDFInfo
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- CN103980010B CN103980010B CN201410242443.1A CN201410242443A CN103980010B CN 103980010 B CN103980010 B CN 103980010B CN 201410242443 A CN201410242443 A CN 201410242443A CN 103980010 B CN103980010 B CN 103980010B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a livestock and poultry manure decomposing inoculant, a use method thereof and a biological organic fertilizer prepared by using the livestock and poultry manure decomposing inoculant and fermenting through a special process. The livestock and poultry manure decomposing inoculant is prepared by compounding the following materials in parts by weight: 0.5-2 parts of bacillus subtilis, 0.5-2 parts of streptomyces microflavus, 0.5-2 parts of trichoderma longibrachiatum and 0.5-2 parts of bacillus mucilagimosus krassilm, wherein the effective viable count is 6*10<8> per gram. The livestock and poultry manure decomposing inoculant disclosed by the invention has multiple functions, can be used for decomposing the livestock and poultry manure and removing the odor and can resist the fusarium oxysporum and increase the yield after being applied to crops. When the livestock and poultry manure decomposing inoculant is applied to treating the livestock and poultry manure, the bacterial count, C/N ratio and fermentation period condition are all better than those of the comparison of an unknown inoculant. The biological organic fertilizer is prepared by adding 1 kilogram of the livestock and poultry manure decomposing inoculant to every 5 squares of the livestock and poultry manure and then fermenting through the special process. The livestock and poultry manure is formed by mixing the following materials in parts by weight: 40-60 parts of chicken manure, 20-40 parts of cattle manure, 5-15 parts of pig manure and 5-15 parts of lignite. The biological organic fertilizer disclosed by the invention is applied to scallion planting, achieves the disease control efficiency up to 56% and increases the yield per mu by 20.5%.
Description
Technical field
The invention belongs to application of the agriculture microorganism on feces of livestock and poultry is processed, it is related to a kind of feces of livestock and poultry and becomes thoroughly decomposed bacterium
Agent and its using method and made biological organic fertilizer.
Background technology
At present, China's Amount of poultry excrements is modern equivalent to 3.4 times of trade waste discharge capacity up to 18.84 hundred million t
10 years afterwards, also by with 8% speed increase, feces of livestock and poultry not processed and applied livestock and poultry breeding industry due to most of, to water
The direct or indirect negative effect that body, air, soil, health and ecosystem are caused, processes and solves feces of livestock and poultry
The environmental problem for causing, is current problem anxious to be resolved.Feces of livestock and poultry production is quickly processed using feces of livestock and poultry decomposing microbial inoculum
Organic fertilizer, is the Main for solving problem above both at home and abroad, and this technology at home and abroad has been obtained for being widely applied.
At present, domestic feces of livestock and poultry decomposing microbial inoculum has often related only to the decomposition of cellulose and protein in feces of livestock and poultry
Ability, function is often more single, and microbial inoculum itself and extension product organic fertilizer performance are single, and added value is not high, agriculturally
Income cannot be improved naturally, so as to affect the smooth solution of problem above.
Based on this, spy proposes the present invention.The invention considers the particularity and extension product of feces of livestock and poultry itself to be had
The disease-resistant performance of the deodoriging properties of the functional characteristic of machine fertilizer, such as decomposing microbial inoculum, decomposing microbial inoculum and its extension product organic fertilizer
With bacterial manure performance etc..
The content of the invention
It is an object of the invention to provide a kind of decomposing microbial inoculum and its using method dedicated for processing feces of livestock and poultry, and
With the microbial inoculum and the bio-organic fertilizer of special process fermenting and producing.
The present invention provide a kind of feces of livestock and poultry decomposing microbial inoculum, it is characterised in that it by decomposable asymmetric choice net cellulose, protein, remove
Smelly, disease-resistant and bacterial manure several functions various bacterial strains are composited, and its active component includes bacillus subtilises（Bacillus
subtilis）, streptomyces microflavus（Streptomyces microflavus）, long handle Trichoderma spp.（Trichoderma
longbrachiatum）And Bacillus mucilaginosus（Bacillus mucilaginosus）, it is by following portions by weight
Each single strain fermented product composition：0.5~2 part of bacillus subtilises, 0.5~2 part of streptomyces microflavus, 0.5~2 part of long handle Trichoderma spp. and
0.5~2 part of Bacillus mucilaginosus；Poultry manure decomposing agent living bacteria count is 6 × 108Individual/g.
Concretely Chinese agriculture Microbiological Culture Collection administrative center deposit number is ACCC to the bacillus subtilises
11089 bacterial strain.Concretely Chinese agriculture Microbiological Culture Collection administrative center deposit number is the streptomyces microflavus
The bacterial strain of ACCC 40027.Concretely Chinese agriculture Microbiological Culture Collection administrative center deposit number is the long handle Trichoderma spp.
The bacterial strain of ACCC 30150.The Bacillus mucilaginosus concretely Chinese agriculture Microbiological Culture Collection administrative center preservation
Numbering is the bacterial strain of ACCC 10013.
The feces of livestock and poultry decomposing microbial inoculum has several functions, including fermentation maturity and deodorization can be carried out to feces of livestock and poultry,
Anti- cucumber fusarium axysporum and crops can be applied to can increase production.Described decomposing microbial inoculum can be used for cattle, pig, chicken, sheep, horse, duck etc.
The quick composting of feces of livestock and poultry, and can in right amount add other organic materials, such as brown coal.
The present invention further discloses the using method of feces of livestock and poultry decomposing microbial inoculum, it is characterised in that enter by the steps
OK：
1st, the carbon-nitrogen ratio of feces of livestock and poultry is adjusted（25~30:1）And moisture（55~60%, with handss one hold handss seam drip for
Preferably）, 1 kilogram of composite bacteria agent capable is added per 5 side's feces of livestock and poultry, turn over and smash uniform.
2nd, by feces of livestock and poultry heap trapezoidal, 1 meter of the heap top width of growth that pretreatment is good, 2 meters of heap bottom width, heap high summer and autumn 0.8~1
Rice, 1.2~1.4 meters of winter-spring season, depending on heap length is according to place, per heap volume 5 sides are no less than.
3rd, after fermentation starts, temperature of charge is worked as（20~30 centimetres of depths）Kept for three days when rising to 55 DEG C, then turn over and smash one
It is secondary.If fermentation initial stage temperature does not rise, moisture is adjusted to recommendation ratio and turned over daily and smash once until heap temperature rises.
4th, after heap temperature rises to again 55 DEG C, kept for three days, then turn over and smash once；
5th, according to heap tender feeling condition carry out turning over smashing afterwards, temperature upward period is not turned over to be smash, temperature stops rising then to turn over smashing, and lays equal stress on
Multiple the step for, when heap temperature is identical with outdoor temperature, moisture is down to below 30%, that is, sweat is completed, whole fermentation
Cycle is usually 15~25 days.
The present invention further discloses poultry manure decomposing microbial inoculum becomes thoroughly decomposed and the application in deodorization in livestock manure fermented, and
Application in arviculture volume increase, wherein being applied to mu volume increase 17.4% in Caulis et Folium Brassicae capitatae plantation.
The invention also discloses a kind of bio-organic fertilizer, it is characterised in that it is sent out by the feces of livestock and poultry decomposing microbial inoculum
Ferment feces of livestock and poultry becomes thoroughly decomposed and forms, wherein adding 1 kilogram of feces of livestock and poultry decomposing microbial inoculum, described feces of livestock and poultry per 5 side's feces of livestock and poultry
Parts by weight it is as follows：40~60 parts of chicken manure；20~40 parts of cattle manure；5~15 parts of pig manure；5~15 parts of brown coal.
The described chicken manure content of organic matter >=45%, total nitrogen content >=2.5%；The cattle manure content of organic matter >=50%；Pig manure is organic
Matter content >=45%；Lignite humic acid content >=50%.
The present invention further discloses the production method of bio-organic fertilizer, it is characterised in that carry out by the steps：
1st, the mixing of raw material：Fully mixed with batch mixer chicken manure, cattle manure, pig manure and brown coal are weighed by weight；
2nd, add water：By water and mixed material 1:1 weight ratio adds water, and control water content is 50~60%；
3rd, feces of livestock and poultry decomposing agent is added：1 kilogram of composite bacteria agent capable is added per 5 side's feces of livestock and poultry；
4th, bank up specification:By regulate water and add composite bacteria agent capable feces of livestock and poultry heap grow up trapezoidal, 1 meter of heap top width,
2 meters of heap bottom width, 0.8~1 meter of heap high summer and autumn, 1.2~1.4 meters of winter-spring season depending on heap length is according to place, are no less than per heap volume
5 sides；
5th, the control of turning time:Kept for three days when 20~30 centimetres of depths temperature of charge rise to 55 DEG C, then turn over and smash
Once, after heap temperature rises to again 55 DEG C, kept for three days, then turn over and smash once, when temperature is more than 70 DEG C, need to be turned over immediately
Smash, suppression of the high temperature to growth of microorganism is eliminated by cooling, so as to promote the activity of a large amount of mesophilic bacterias, accelerate entering for compost
Journey；
6th, turning is required：Turn over and to accomplish during pile tune, even, broken, it is exactly that original top section in pile is transferred to earth's surface to adjust
Become underclad portion, and underclad portion is transferred to top layer and is changed into top section, to reach the purpose of abundant fermentation；Even is exactly in pile
Raw material mixes also uneven part, and mixing work is carried out again；Broken is exactly the massive zymolysis thing to being formed during the fermentation
Carry out broken work；
7th, the addition of object bacteria：After fermentation the phase, i.e., when fermentation starts cooling, add Bacillus mucilaginosus single strain
Microbial inoculum, is 1kg per square object material addition；
8th, fermentation termination control：When heap temperature is identical with outdoor temperature, moisture is down to less than 30%, that is, complete biological organic
The whole fermentation production process of fertilizer, whole cycle is usually 15~20 days, 25~30 days time in winter；
9th, fermented quality control：Crushed after the completion of fermentation, sieved, detected, weighed and packed, bio-organic fertilizer is produced
Product production is completed, and bio-organic fertilizer organic matter requires to reach 45%~55%, N, P, K total nutrient 5%, and living bacteria count ＞ 0.2 ×
108Individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
The present invention further discloses application of the biological organic fertilizer in terms of vegetable disease-resistant effect of increasing production.The biological organic
Fertilizer is applied to have obvious disease-resistant yield-increasing effect in Herba Alii fistulosi plantation, and preventive effect is increased production up to 20.5% up to 56%.
The decomposing microbial inoculum energy quick composting feces of livestock and poultry that the present invention is provided, with good deodoriging properties, and then output is high
Quality can be disease-resistant bio-organic fertilizer, can avoid because feces of livestock and poultry becomes thoroughly decomposed that the pest and disease damage that thoroughly do not brought is high, emergence rate
The disadvantage such as low, burn seedlings, dead seedling, weeds are more.Can significantly be increased by the become thoroughly decomposed bio-organic fertilizer product of production of described decomposing microbial inoculum
Plus the content of the nutrient such as the soil organism and phosphorus, potassium, quantity of useful microbe in nutrient conversion efficiency and product is improved, promote
Soil remediation, culture fertility can reduce fertilizer application amount, while strengthening the disease resistance of crop, improve crop quality, reduce and throw
Enter, improve yield, promote to increase income.
Description of the drawings：
Fig. 1 is the preparation technology flow chart of bio-organic fertilizer.
Specific embodiment
Below example facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method, if no special instructions, is conventional method.Test material used in following embodiments, if no special instructions, is certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following examples, is respectively provided with three repetitions and tests, and as a result makes even
Average.
Following bacterial strain is all obtained from Chinese agriculture Microbiological Culture Collection administrative center (Agricultural Culture
Collection of China, abbreviation ACCC)：
Bacillus subtilises（Bacillus subtilis）：ACCC deposit numbers are 11089, abbreviation ACCC11089；
Streptomyces microflavus（Streptomyces microflavus）：ACCC deposit numbers are 40027, abbreviation ACCC
40027；
Long handle Trichoderma spp.（Trichoderma longbrachiatum）：ACCC deposit numbers are 30150, referred to as
ACCC30150；
Bacillus mucilaginosus（Bacillus mucilaginosus）：ACCC deposit numbers are 10013, referred to as
ACCC10013。
Embodiment 1：
The preparation of culture medium and strain
First, the preparation of culture medium
1st, Rhizoma Solani tuber osi, glucose agar medium（PDA）
200 grams of the Rhizoma Solani tuber osi of skin is removed, is cut into small pieces, the 1000ml that adds water boils 30 minutes, potato ball is filtered off, by filtrate
1000ml, plus 20 grams of glucose are complemented to, 15 grams of agar dissolves rear subpackage, and 15 pounds sterilize 30 minutes, and pH natures prepare liquid
Agar is not added with during culture medium.
2nd, nutrient agar
It is made up of peptone 5g, Sodium Chloride 5g, Carnis Bovis seu Bubali cream 3g and distilled water 1000ml.pH7.0（Solid medium adds fine jade
Fat 15-20g）.
3rd, Gao Shi synthesizes No. 1 culture medium
KNO3 1g, K2HPO4 0.5g, NaCl 0.5g, FeSO47H2O 0.01g, MgSO47H2O 0.5g soluble starches
20g, pH7.2~7.4, distilled water 1000ml.
4th, B. mucilaginocus culture medium
Yeast extract 0.4g, Mannitol 10.0g, K2HPO4 0.5g、MgSO47H2O 0.2g、FeCl3 0.005g、CaCO3
1.0g, distilled water 1000.0ml, PH7.0-7.2, when preparing solid medium 15 grams of agar are added.
5th, solid fermentation culture medium
Testa Tritici 80g, bean cake 10g, (NH4)2SO41.0g, KH2PO40.2g, MgSO4·7H200.05g, 80mL distilled water.
2nd, the activation of strain
Bacillus subtilises bacterium is seeded in nutrient agar culture medium；Long handle Trichoderma spp. is seeded in PDA culture medium；Carefully
Yellow streptomycete is seeded in Gao Shi and synthesizes in No. 1 culture medium；Bacillus mucilaginosus are seeded in B. mucilaginocus culture medium
Bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp. and Bacillus mucilaginosus are carried out into respectively inclined-plane switching, is connect
Enter respective ramp culture medium, activation culture 3-7 days.
3rd, prepared by single strain
1st, the culture and fermentation of bacillus subtilises
Slant medium and Shake flask medium are all nutrient agar, and when preparing solid medium 15 grams of agar are added, and are planted
Sub- tank and fermentation tank culture medium are 1000kg water, the bean milk of 8.75kg Semen sojae atricolor, the starch of 6.5kg, 1kg glucoses,
CaCO3100g, FeCl310g, pH7.0, Jing after 121 DEG C of real tanks sterilize 30 minutes, cultivate 24~36 hours at 28 DEG C~30 DEG C, when
Bacterium number reaches 10 × 10 in per ml fermentation liquids8Individual/g.Then the fermentation liquid by weight respectively than 3 parts is adsorbed on 1 part of Vermiculitum
Room temperature dries and obtain bacillus subtilises single strain microbial inoculum.
2nd, the culture and fermentation of Bacillus mucilaginosus
Slant medium and Shake flask medium are all B. mucilaginocus culture medium, and seed tank and fermentation tank culture medium are 1000kg
Water, the bean milk of 8.75kg Semen sojae atricolor, the starch of 6.5kg, 1kg glucoses, CaCO3100g, FeCl3The real tanks of 121 DEG C of 10g, pH7.0, Jing
After sterilizing 30 minutes, cultivate 48 hours at 28 DEG C~30 DEG C, when bacterium number reaches 5 × 10 in every ml fermentation liquids8Individual/g.Then press and divide
Fermentation liquid of the other weight than 3 parts is adsorbed in room temperature on 1 part of Vermiculitum and dries and obtain Bacillus mucilaginosus single strain microbial inoculum.
3rd, the culture and fermentation of long handle Trichoderma spp.
Inclined-plane and Shake flask medium are Rhizoma Solani tuber osi, glucose agar medium, and slant medium adds agar.Liquid culture
During 500ml triangular flasks are sub-packed in after base is sterilized, each funguses slant strains of access, shaking table speed 180r/min, 28 DEG C~30 DEG C
Culture 48 hours, big when occurring density in shaking flask, mycelium pellet is not substantially muddy.Seed tank and fermentation cylinder for fermentation culture medium
For the cooking liquor of 1000kg water, the bean milk of 8.75kg Semen sojae atricolor, the starch of 6.5kg, 1kg glucoses and 2kg Rhizoma Solani tuber osis, pH7.0, Jing
After 121 DEG C of real tanks sterilize 30 minutes, cultivate 48 hours at 28 DEG C~30 DEG C, after density big mycelium pellet occurs in fermentation liquid, connect
Plant in solid fermentation culture medium, inoculum concentration 10%, 121 DEG C, 0.1MPa autoclaving 30min, 25 DEG C of solid fermentation culture 48h,
Spore count is up to 6 × 108Individual/g.
4th, the culture and fermentation of streptomyces microflavus
Inclined-plane and Shake flask medium are that Gao Shi synthesizes No. 1 culture medium, and slant medium adds agar.Fluid medium Jing is gone out
During 500ml triangular flasks are sub-packed in after bacterium, each funguses slant strains are accessed, shaking table speed 180r/min, 28 DEG C~30 DEG C cultures 5~
7 days, big when occurring density in shaking flask, mycelium pellet was not substantially muddy.Seed tank and fermentation cylinder for fermentation culture medium are
The cooking liquor of 1000kg water, the bean milk of 8.75kg Semen sojae atricolor, the starch of 6.5kg, 1kg glucoses and 2kg Rhizoma Solani tuber osis, pH7.0, Jing 121
After DEG C real tank sterilizes 30 minutes, cultivate 48 hours at 28 DEG C~30 DEG C, after density big mycelium pellet occurs in fermentation liquid, be inoculated into
In solid fermentation culture medium, inoculum concentration 10%, 121 DEG C, 0.1MPa autoclaving 30min, 25 DEG C of solid fermentation cultures 5~7 days,
Spore count is up to 6 × 108Individual/g.
4th, decomposing microbial inoculum（Represented with composite bacteria agent capable below）Preparation
Bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp. and the Bacillus mucilaginosus list bacterium produced as stated above
It is 0.5~2 to plant weight ratio:0.5~2:0.5~2:0.5~2.Mixing is obtained composite bacteria agent capable after mixing thoroughly, and composite bacteria agent capable is effectively lived
Bacterium number reaches 6 × 108Individual/g.
Embodiment 2：
Composite bacteria agent capable is constituted
A kind of feces of livestock and poultry decomposing microbial inoculum, is formulated as follows（By weight）：
The composition of the feces of livestock and poultry decomposing microbial inoculum of table 1
| Title | Bacillus subtilises | Streptomyces microflavus | Long handle Trichoderma spp. | Bacillus mucilaginosus |
| （1） | 0.5 | 2.0 | 1.0 | 2.0 |
| （2） | 1.0 | 1.5 | 2.0 | 1.5 |
| （3） | 1.5 | 1.0 | 1.5 | 1.0 |
| （4） | 2.0 | 0.5 | 0.5 | 0.5 |
Wherein living bacteria count is 6 × 108Individual/g；
The proportioning of the bio-organic fertilizer raw materials for production of table 2（Weight percent）
| Title | Chicken manure（%） | Cattle manure（%） | Pig manure（%） | Brown coal（%） |
| （1） | 40 | 40 | 10 | 10 |
| （2） | 45 | 30 | 15 | 10 |
| （3） | 50 | 30 | 5 | 15 |
| （4） | 60 | 20 | 15 | 5 |
Embodiment 3：
Mutual antagonistic experiment between bacterial strain
Bacillus subtilises after activation, streptomyces microflavus, long handle Trichoderma spp. and Bacillus mucilaginosus are carried out between bacterial strain
Mutual antagonistic experiment, combination of two is divided into into 12 kinds of compound modes, each three repetition of combination.
Bacteriostatic test is carried out using steel ring method, bacterial strain is cultivated 3-7 days in corresponding culture medium（190r/min, 28 DEG C）System
Into fermentation liquid.Take one of which bacterium（A）Fermentation liquid 0.1mL be applied on PDA plate, then in the solid medium of each flat board
On put the steel rings of two in advance sterilizings, then any bacterium outside the A bacterium of 0.25mL is added in each steel ring（B）Fermentation
Liquid, each is processed in triplicate, only to connect the plate of A bacterium as control.28 DEG C of cultures and routine observation antibacterial situation.The results are shown in Table
3。
Antagonistic effect between the strain of table 3
| Bacillus subtilises | Streptomyces microflavus | Long handle Trichoderma spp. | Bacillus mucilaginosus | |
| Bacillus subtilises | - | - | - | |
| Streptomyces microflavus | - | - | - | |
| Long handle Trichoderma spp. | - | - | - | |
| Bacillus mucilaginosus | - | - | - |
Note："-" represents that Antagonistic reaction is negative, and "+" represents that Antagonistic reaction is positive.
Result of the test shows between bacillus subtilises, streptomyces microflavus, 4 kinds of bacterium of long handle Trichoderma spp. and Bacillus mucilaginosus
Unrestraint is acted on, and Antagonistic reaction is feminine gender.
Embodiment 4：
The cellulose and the measure of breaks down proteins ability of single bacterial strain and composite bacteria agent capable
First, cellulase activity assay method
1st, reagent and solution
Substrate solution：0.625 g sanloses are accurately weighed, 100.0 mL sodium phosphate buffers (0.2 are dissolved in
M, pH 6.0）, heated and stirred is allowed to dissolve.
DNS developers：10.0 g 3 are weighed, 5- dinitrosalicylic acids are dissolved in distilled water, add 20.0 g hydroxides
Sodium, 200.0 g sodium potassium tartrate tetrahydrates and 500.0 ml water, add the g of re-distilled phenol 2.0, anhydrous sodium sulfite 0.5 after heating for dissolving
G is cooled down after all dissolvings, is dissolved to 1000.0 mL.
2nd, instrument and equipment
Spectrophotometer, electronic balance, agitator incubator.
3rd, Specification Curve of Increasing
Standard glucose solution：25.0mL is settled to distillation water dissolution 25.0mg glucoses.
5 test tubes with 20.0 ml scales are taken, by upper table reagent is measured.Often respectively add the M hydrogen-oxygens of 1.0 ml 2.0 in pipe
Change sodium solution and 2.0 ml DNS nitrite ions, after accurately 5.0 min of heating are placed in boiling water bath after shaking up, flowing water cooling, distillation
Water is settled to 20.0 ml, shakes up the OD values that each pipe is determined at 490 nm, and as abscissa, OD values are the μ g numbers with glucose
Vertical coordinate, draws standard curve.
The Specification Curve of Increasing table of table 4
| Test tube number | Standard glucose solution（ml） | Sodium phosphate buffer (0.2M pH 6.0)（ml） | Glucose amount in test tube（μg） |
| 1 | 0 | 5 | 0 |
| 2 | 0.4 | 4.6 | 400 |
| 3 | 0.7 | 4.2 | 800 |
| 4 | 1.6 | 3.4 | 1600 |
| 5 | 3.2 | 1.8 | 3200 |
4th, former state enzyme liquid
10 times of 5.0g samples distilled water diluting is weighed, fully vibration, static 30min is filtered, and its filtrate is former state enzyme
Liquid.
5th, determination step
Take 3 test tubes with 20.0ml scales, 1 as blank, remaining 2 used as parallel sample pipe.Sample
Guan Zhongjia 1.0ml former state enzyme liquids, are then separately added into the substrate solution that 4.0ml is pre-heated to 60 DEG C, at 60 DEG C in 3 test tubes
20min is reacted in water-bath to take out, the 2.0M sodium hydroxide solutions and 2.0ml DNS nitrite ions for adding 1.0ml immediately is often managed, and is shaken
1.0ml former state enzyme liquids are added in control tube after even.3 test tubes are put in boiling water bath, are taken out immediately after colour developing 5min, flowed
Water cooling, is dissolved to 20.0ml, and its OD value is surveyed at 490nm with spectrophotometer.
6th, cellulase activity is calculated:
Glucose μ g numbers, U=are converted into according to standard curve（M1-M0）/20
In formula：U：The enzyme activity of sample（μg/min）； M0：Control glucose amount（μg）
M1：Example weight after decomposition（μg）； 20：The enzyme-to-substrate response time（min）
1ml former state enzyme liquids, the μ g glucoses of generation per minute 1 are defined as 1 enzyme activity unit（u）.
2nd, proteinase activity assay method
1st, reagent：According to the regulation in QB/T 1803.
2nd, the preparation of former state enzyme liquid
Weigh sample 10.0g（Or 10.0ml）, add the triangular flask equipped with bead（150ml）In, add certain body
Long-pending phosphate buffer（pH7.5）Dissolving, 200 r/min shaking tables vibration 30min, then four layers of filtered through gauze, filtrate then at
3000r/min is centrifuged 20min.Supernatant after centrifugation is according to enzyme activity buffer（pH7.5）Debita spissitudo is diluted to, for surveying
It is on probation.
3rd, determination step：Other simply have in concrete measure according to the regulation of QB/T1803 and change at two：One is reaction
Time（After adding casein）30min is changed to by 10min；Two is that former static filtration is changed to that 5min filtrations are centrifuged.
3rd, enzyme assay result
Bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp., Bacillus mucilaginosus and composite bacteria agent capable will be prepared by upper
The method of stating carries out cellulase and protease activity determination, and measurement result see the table below.
The enzyme activity implementations of table 5
| Process | Cellulase activity (u/ml) | Proteinase activity (u/ml) |
| Bacillus subtilises | 68.00 | 47.20 |
| Streptomyces microflavus | 12.51 | - |
| Long handle Trichoderma spp. | 95.08 | 15.74 |
| Bacillus mucilaginosus | 15.64 | 8.94 |
| Composite bacteria agent capable | 60.08 | 21.37 |
Testing result shows, by bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp., 4 kinds of bacterial strains of Bacillus mucilaginosus
The composite bacteria agent capable of composition has very strong cellulose and breaks down proteins ability, higher than organic matter decomposing inoculant national Specification
Cellulase activity 30 (u/ml) and proteinase activity 15 (u/ml) requirement, composite bacteria agent capable can be used as organic material composting
Agent is used.
Embodiment 5：
Single bacterial strain is determined with the fermentative deodorizing ability of composite bacteria agent capable
First, agents useful for same and fermentation substrate
1st, sulfuric acid solution：Sulphuric acid 0.005mol, with distilled water 1L is settled to.
2nd, starch indicator solution：1g starch adds distilled water 10ml, in injecting the distilled water that 200ml boils, micro-boiling 2min.
3rd, bromocresol green ethanol solution：0.10g bromocresol greens, with ethanol 100ml is settled to.
4th, C.I. 13020 .-bromocresol green indicator：The 0.1% bromocresol green ethanol solution and 0.2% methyl of 1 volume of 3 volumes
Red ethanol solution mixing.
5th, acetic acid zinc solution：Weigh 50g zinc acetates to be dissolved in a small amount of distilled water, add 2 to drip glacial acetic acid, stir to solution and become
It is limpid, add 2 to drip ethanol, it is settled to 1L with distilled water.
6th, C.I. 13020. ethanol solution is prepared：0.20g C.I. 13020 .s, with ethanol 100ml is settled to.
7th, boric acid solution：20.41g boric acid and 1000ml distilled water.
8th, iodine standard solution：13g iodine is dissolved in 100mL distilled water with 35g potassium iodide, and with distilled water 1000mL is settled to,
Shake up and be stored in brown bottle.
9th, hypo solution：The water sodium thiosulfate of 13g five and 0.5g sodium carbonate, are settled to 1L, slowly with distilled water
10min is boiled, in being contained in brown bottle.
10th, hydrochloric acid（1+1）Solution：By 1 volume of water and the hydrochloric acid of 1 volume 37%.
11st, fermentative deodorizing substrate：It is made up of dried poultrymanure 60g, powder of straw 10g and water, water content is 50%.
2nd, assay method
The main component of the foul smell that compost is produced is ammonia and hydrogen sulfide, and the harm to people and animals is very big, main in embodiment
The deodorizing capability of microbial bacterial agent is evaluated using ammonia detection, sulfurated hydrogen detection and organoleptic detection.
1st, ammonia detection method
The ammonia in gas is absorbed with 2% boric acid solution, is then titrated with 0.005mol/L sulfuric acid solutions, first dripped during titration
Methylate is red-and bromocresol green mixed indicator 5-6 drops are in the boric acid solution for absorbing ammonia then molten with 0.005mol/L sulphuric acid
Drop is fixed to arrive terminal up to pink, according to the consumption of sulfuric acid solution（Volume）Calculate ammonia level.
Reaction equation is：(1)NH3+H3BO3→NH4H2BO3；(2)NH4H2BO3+H2SO4→(NH4)2SO4+2H3BO3。
The computing formula of the amount of the material of ammonia：n1=0.005*（V1-V01）*10-3；n1The amount of the material of-ammonia
（mol）, the substance withdrawl syndrome of 0.005-sulphuric acid（mol/L）, V1The consumption of sulfuric acid solution when-test solution is titrated（ml）, V01—
The consumption of sulfuric acid solution during blank titration（ml）.
2nd, sulfurated hydrogen detection method
Iodimetric titration is, with the hydrogen sulfide in acetic acid zinc-iron alloy solution unstripped gas, to add excess iodine standardized titration molten in slightly acidic solution
Liquid is S2-Simple substance S is oxidized to, remaining iodine is titrated with hypo solution, according to hypo solution consumption, is calculated
Hydrogen sulfide content；By 5mL iodine standard solutions, 5mL hydrochloric acid（1+1）Solution adds the 50g/L zinc acetate solutions for absorbing hydrogen sulfide
In, confined reaction 5 minutes is titrated, the starch indicator of Deca 0.5% during nearly terminal, extremely with 0.05mol/L hypo solutions
Become colorless, the volume of record hypo solution used, you can calculate the content of hydrogen sulfide.
The reaction equation of iodometric titrationiodimetry titration：（1）H2S+Zn2+→ZnS↓+2H+；(2)ZnS+I2→Zn2++2I-+S↓；(3)I2+
2S2O3 2-→2I-+S4O6 2-。
The computing formula of the amount of the material of hydrogen sulfide：n2=[0.5-0.05*（V02-V2）/2]*10-3；n2The thing of-hydrogen sulfide
The amount of matter（mol）, the amount of the material of 0.5-standard iodine solution（mol）, the substance withdrawl syndrome of 0.05-hypo solution
（mol/L）, V02The consumption of hypo solution during-blank titration（ml）, V2Hypo solution when-test solution is titrated
Consumption（ml）.
3rd, experimental technique
Bacillus subtilises after activation, Bacillus licheniformis, sporotrichum thermophile, aspergillus oryzae and Trichoderma viride are used respectively
Fluid medium culture 2 days, obtains the bacterium solution of each bacterial strain.
The bacterium solution and composite bacteria agent capable of each bacterial strain are tested as follows respectively：Bacterium solution is connected on into fermentative deodorizing Medium Culture,
Mix in 500mL beakers（Every part of fermentative deodorizing substrate is inoculated with 10mL bacterium solutions）, as experiment sample, fermentative deodorizing substrate is set
Used as check sample, each sample kraft paper and one layer of plastic paper are sealed.Each sample（Six kinds of experiment samples and a kind of control sample
This）Three parallel processing are set, and each parallel processing arranges three repetitions, as a result takes the meansigma methodss of three repetitions.At first
Contain in parallel middle placement 20mL 2% boric acid solution small beaker to absorb sweat in the ammonia that produces, the 7th day with acid
Titration measuring；Produce during the 50g/L acetic acid zinc solutions of 20mL are contained in second parallel middle placement to absorb sweat
Hydrogen sulfide, use iodometric determination within the 7th day；3rd parallel, carries out sense organ measure within the 7th day.
4th, experimental result
The results are shown in Table 6.
The fermentative deodorizing ability of table 6
| Process | Foul smell grade | Ammonia level（10-3mol） | Hydrogen sulfide content（10-3mol） |
| Bacillus subtilises | + | 0.00260 | 0.32321 |
| Streptomyces microflavus | + | 0.00250 | 0.32509 |
| Long handle Trichoderma spp. | +++ | 0.00325 | 0.43461 |
| Bacillus mucilaginosus | ++ | 0.00310 | 0.40098 |
| Composite bacteria agent capable | + | 0.00220 | 0.30765 |
| Control | ++++ | 0.00713 | 0.65432 |
Note：How much expression sense organs of "+" determine foul smell grade, and "+" represents that sense organ measure is more smelly.
Show with reference to the testing result of organoleptic detection and ammonia and hydrogen sulfide content, by bacillus subtilises, thin Huang strepto-
The composite bacteria agent capable deodorizing effect of bacterium, long handle Trichoderma spp. and Bacillus mucilaginosus composition is significantly better than single bacterial strain and control.
Embodiment 6：
Single bacterial strain is determined with the resistances against diseases of composite bacteria agent capable
Bacteriostatic test is carried out using steel ring method, by the bacillus subtilises after activation, streptomyces microflavus, long handle Trichoderma spp., glue
Peptone sample bacillus cereuss and composite bacteria agent capable carry out bacteriostatic test with cucumber fusarium axysporum respectively, each three repetition of combination.
Each bacterial strain is cultivated 2-3 days in corresponding culture medium（190r/min, 28 DEG C）Fermentation liquid is made, by cultured Huang
Cucurbit wilt bacterium makes spore concentration for 106The bacteria suspension of individual/mL.Take cucumber fusarium axysporum bacteria suspension 0.1mL and be applied to PDA plate
On, the steel ring of two sterilizings in advance is then put on the solid medium of each flat board, then add in each steel ring
Above-mentioned each fermented liquid of 0.25mL and composite bacteria liquid, each is processed in triplicate, only to connect cucumber fusarium axysporum bacteria suspension
Plate is control.28 DEG C of cultures and routine observation antibacterial situation.
The each bacterial strain of table 7 and bacteriostasis of the composite bacteria agent capable to cucumber fusarium axysporum
Note："+" represents that Antagonistic reaction is positive, and "+" represents that inhibition is bigger.
Result of the test shows, bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp., Bacillus mucilaginosus and compound bacteria
Agent has fungistatic effect to cucumber fusarium axysporum, and wherein composite bacteria agent capable is most strong.
Embodiment 7：
The effect of increasing production of single bacterial strain and composite bacteria agent capable
1st, test site and varieties of plant
Test vegetable is Caulis et Folium Brassicae capitatae.
2nd, field design
Totally 6 process, each processes 3 repetitions, conventional fertilizer application, plot area 96m2。
Process 1:Bacillus subtilises；Process 2：Streptomyces microflavus；Process 3：Long handle Trichoderma spp.；Process 4：Glue peptone sample spore
Bacillus；Process 5：Composite bacteria agent capable；Process 6：Control.
3rd, result of the test
The each bacterial strain of table 8 and composite bacteria agent capable field effect of increasing production
| Process | Cell average product (kg) | Per mu yield（kg） | Volume increase（%） |
| Process 1 | 480 | 3335 | 14.3 |
| Process 2 | 482 | 3349 | 14.8 |
| Process 3 | 478 | 3321 | 13.8 |
| Process 4 | 490 | 3404 | 16.7 |
| Process 5 | 495 | 3425 | 17.4 |
| Process 6 | 420 | 2918 | - |
Result of the test shows, bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp., Bacillus mucilaginosus and compound bacteria
Agent has effect of increasing production on Caulis et Folium Brassicae capitatae is planted than control, and wherein composite bacteria agent capable is optimal, better than single bacterial strain, can exist as bacterial manure
Used in farmland.
Embodiment 8：
The using method and effect of composite bacteria agent capable
By above example, illustrate by bacillus subtilises, streptomyces microflavus, long handle Trichoderma spp., Bacillus mucilaginosus 4
The composite bacteria agent capable of bacterium composition is planted, the effect with several functions can carry out the quick of feces of livestock and poultry as organic matter decomposing inoculant
Become thoroughly decomposed, the deodorization of feces of livestock and poultry can be carried out as deodorizer, the preventing and treating of crop pest can be carried out as disease-resistant microbial inoculum, can also make
For the cultivation that agricultural microbial agent is applied to crops, play a part of volume increase.
First, the using method of composite bacteria agent capable
1st, the carbon-nitrogen ratio of feces of livestock and poultry is adjusted（25~30:1）And moisture（55~60%, with handss one hold handss seam drip for
Preferably）, 1 kilogram of composite bacteria agent capable is added per 5 side's feces of livestock and poultry, turn over and smash uniform.
2nd, by feces of livestock and poultry heap trapezoidal, 1 meter of the heap top width of growth that pretreatment is good, 2 meters of heap bottom width, heap high summer and autumn 0.8~1
Rice, 1.2~1.4 meters of winter-spring season, depending on heap length is according to place, per heap volume 5 sides are no less than.
3rd, after fermentation starts, temperature of charge is worked as（20~30 centimetres of depths）Kept for three days when rising to 55 DEG C, then turn over and smash one
It is secondary.If fermentation initial stage temperature does not rise, moisture is adjusted to recommendation ratio and turned over daily and smash once until heap temperature rises.
4th, after heap temperature rises to again 55 DEG C, kept for three days, then turn over and smash once；
5th, according to heap tender feeling condition carry out turning over smashing afterwards, temperature upward period is not turned over to be smash, temperature stops rising then to turn over smashing, and lays equal stress on
Multiple the step for, when heap temperature is identical with outdoor temperature, moisture is down to below 30%, that is, sweat is completed, whole fermentation
Cycle is usually 15~25 days.
2nd, the using effect of composite bacteria agent capable
1st, materials and methods
Composting material：Chicken manure, cattle manure, pig manure and brown coal.Weight ratio accounts for 40~60% for chicken manure；Cattle manure accounts for 20~40%；Pig
Excrement 5~15%；Brown coal 5~15%.C/N ratios are 37.
If two process, are processed：Plus the invention feces of livestock and poultry decomposing microbial inoculum；Control:Not with the blank of microbial inoculum, often
Individual process 2T raw materials, three repetitions are averaged, pile type fermentation reactor system.Fermentation period room temperature is down to by fermentation temperature in terms of
Gained is calculated, fermentation is processed two and is sampled analysis, including available N, P, K total amount on the 16th day（%）, C/N ratios, bacterium number and large intestine bar
Bacterium, averages, and observes the physical state of fermentation, as a result as shown in the table:
2nd, result and analysis
The composite bacteria agent capable of table 9 becomes thoroughly decomposed and deodorizing effect
As shown in Table 9, from the point of view of indices, process ferment effect and be better than control, wherein process fermentation temperature is in 2 days
55 DEG C can be reached, control needs 5 days；The result of fermentation shows as processing micro organism quantity growth and breeding to be enlivened, bacterium number and speed
Effect N, P, K content is above as shown in table 5 control, and fermentation period is also greatly shortened, wherein being processed as 16 days, compares 28 days, place
Haircut ferment color pitchy, odorless, and it is light color to compare, and has stink.Fermentation post processing in 16 days and control C/N ratios are respectively
18.6th, than lower, microorganism acts on better to 23.0, C/N to feces of livestock and poultry cellulose degradation.Therefore, from bacterium number, available nutrient,
From the point of view of C/N ratios, abnormal smells from the patient and fermentation period effect, illustrate to add the composite bacteria agent capable to feces of livestock and poultry become thoroughly decomposed and deodorization is all played
Facilitation effect.
Embodiment 9：
Made biological organic fertilizer and Field information effect are produced using composite bacteria agent capable
Under the conditions of certain site facility, there is biodegradation, chemical degradation by adding decomposing microbial inoculum, feces of livestock and poultry
And physical change, produce CO2、H2O and NH3Deng gas and discharge heat, generating can absorb small molecule by crop conversion in a large number
Inorganic and organic nutrient substance, form stable physical arrangement and state, obtain bio-organic fertilizer of the present invention and produce
Product.
First, the production of biological organic fertilizer and technique
1st, raw material and formula：Biological organic fertilizer raw materials for production include chicken manure, cattle manure, four kinds of organic materials of pig manure and brown coal.
By weight, chicken manure accounts for 40~60%；Cattle manure accounts for 20~40%；Pig manure 5~15%；Brown coal 5~15%.
2nd, material choice：The chicken manure content of organic matter >=45%, total nitrogen content >=2.5%；The cattle manure content of organic matter >=50%；Pig
The excrement content of organic matter >=45%；Lignite humic acid content >=50%.
3rd, proportioning raw materials：Weigh by weight 40~60% chicken manure, 20~40% cattle manure, 5~15% pig manure and 5~
15% brown coal.
4th, the mixing of raw material：The dried poultrymanure, dry cow dung, dry pig manure and brown coal that weigh by weight are filled with batch mixer
Divide and mix.
5th, add water：By water:Mixed material 1:1 weight ratio adds water, and control water content is 50~60% or so.
6th, composite bacteria agent capable of the present invention is added：1 kilogram of composite bacteria agent capable is added per 5 side's feces of livestock and poultry
7th, bank up specification：By regulate water and add composite bacteria agent capable feces of livestock and poultry heap grow up trapezoidal, 1 meter of heap top width,
2 meters of heap bottom width, 0.8~1 meter of heap high summer and autumn, 1.2~1.4 meters of winter-spring season depending on heap length is according to place, are no less than per heap volume
5 sides.
8th, the control of turning time：After fermentation of banking up starts, if fermentation initial stage temperature does not rise, such as moisture content not enough, is then adjusted
Whole moisture such as moisture content excess, then must daily be turned over and smash once to recommendation ratio 50~60%, increase oxygen content in pile, promote micro-
Biological fast-growth, until heap temperature rises.Work as temperature of charge（20~30 centimetres of depths）Kept for three days when rising to 55 DEG C, then turned over
Smash once, harmful pathogenic microorganism such as excrement enterobacteria and roundworm egg and worm's ovum are killed with profit.After heap temperature rises to again 55 DEG C, protect
Hold three days, then turn over and smash once.Carry out turning over smashing according to heap tender feeling condition afterwards, temperature upward period is not turned over to be smash, temperature stops rising
Then turn over and smash, when temperature is more than 70 DEG C, need to carry out turning over immediately smashing, suppression of the high temperature to growth of microorganism is eliminated by cooling, from
And promote the activity of a large amount of mesophilic bacterias, accelerate the process of compost.
9th, turning is required：Turn over and to accomplish during pile tune, even, broken.Adjust is exactly that original top section in pile is transferred to earth's surface
Become underclad portion, and underclad portion is transferred to top layer and is changed into top section, to reach the purpose of abundant fermentation；Even is exactly in pile
Raw material mixes also uneven part, and mixing work is carried out again；Broken is exactly the massive zymolysis thing to being formed during the fermentation
Carry out broken work.
10th, the addition of object bacteria：After fermentation the phase, i.e., when fermentation starts cooling, add Bacillus mucilaginosus list bacterium
Strain microbial inoculum, is 1kg per square object material addition.
11st, fermentation termination control：When heap temperature is identical with outdoor temperature, moisture is down to less than 30%, outward appearance pitchy, odorless,
The whole fermentation production process of biological organic fertilizer is completed, whole cycle is usually 15~20 days, 25~30 days time in winter.
12nd, fermented quality control：Crushed after the completion of fermentation, sieved, detected, weighed and packed, bio-organic fertilizer
Production is completed.Bio-organic fertilizer organic matter requires to reach 45%~55%, N, P, K total nutrient 5%, living bacteria count ＞ 0.2
×108Individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
Because the composite bacteria agent capable collects cellulose decomposition, breaks down proteins, deodorization, disease-resistant and bacterial manure several functions in one
Body, after adding feces of livestock and poultry material, on the one hand probioticss decompose the feces of livestock and poultry that becomes thoroughly decomposed and form stable biological organic fertilizer, on the one hand
Itself expanding propagation is carried out using feces of livestock and poultry, own disease-resistant and bacterial manure function is transplanted in the biological organic fertilizer for generating,
The biological organic fertilizer for making generation is equally provided with the multiple performances such as disease-resistant and bacterial manure, sees technological process Fig. 1.
2nd, using method
200~500 kilograms are applied with per mu as base manure, is spread fertilizer over the fields before ploughing and earth's surface, then uniformly apply with being ploughed
Use as base manure in soil；Trench digging is sprinkled into fertilizer in field of vegetables during ditch spread, then moves seedling or dibbling, 200 kilograms of left sides of mu consumption
It is right；Dibbling or when transplanting, can take the method spreaded manuer in holes, and can be put in every cave after the mixing of fertilizer and fine earth, then transplant again or
Dibbling, 10 kilograms or so of mu consumption.
3rd, Field information effect
Trial crops are Herba Alii fistulosi, and soil situation is available nitrogen 73PPm, rapid available phosphorus 33PPm, available potassium 165PPm, organic matter
1.85%, total salt 0.117%, pH7.89, plot area 40m2, test area is 0.36 mu, 10 mu of area of demonstrating.
EXPERIMENTAL DESIGN：If two process：1st, conventional fertilizer application+biological organic fertilizer, 2, conventional fertilizer application（Control）；Three repetitions,
Order is arranged.Result of the test is as shown in table 10：
The biological organic fertilizer Field information effect of table 10
Result of the test shows that biological organic fertilizer of the present invention has obvious disease-resistant yield-increasing effect, and preventive effect increases up to 56%
Produce up to 20.5%.
Claims (2)
1. a kind of preparation method of bio-organic fertilizer, its feature is carried out by the steps：
（1）The mixing of raw material：Fully mixed with batch mixer dried poultrymanure, dry cow dung, dry pig manure and brown coal are weighed by weight,
The parts by weight of described feces of livestock and poultry are as follows：40~60 parts of chicken manure；20~40 parts of cattle manure；5~15 parts of pig manure；Brown coal 5~15
Part；The wherein chicken manure content of organic matter >=45%, total nitrogen content >=2.5%；The cattle manure content of organic matter >=50%；The pig manure content of organic matter
≥45%；Lignite humic acid content >=50%.
（2）Add water：By water and mixed material 1:1 weight ratio adds water, and control water content is 50~60%；
（3）Add feces of livestock and poultry decomposing agent：1 kilogram of composite bacteria agent capable, described feces of livestock and poultry is added to become thoroughly decomposed bacterium per 5 side's feces of livestock and poultry
Agent is composited by decomposable asymmetric choice net cellulose, protein, deodorization, disease-resistant and bacterial manure several functions various bacterial strains, its activity into
Dividing includes bacillus subtilises（Bacillus subtilis）, streptomyces microflavus（Streptomyces microflavus）, it is long
Handle Trichoderma spp.（Trichoderma longbrachiatum）And Bacillus mucilaginosus（Bacillus mucilaginosus）, it
It is made up of each single strain fermented product of following portions by weight：0.5~2 part of bacillus subtilises, 0.5~2 part of streptomyces microflavus,
0.5~2 part of 0.5~2 part of long handle Trichoderma spp. and Bacillus mucilaginosus；Poultry manure decomposing agent living bacteria count is 6 × 108Individual/g；
（4）Bank up specification:By regulate water and add composite bacteria agent capable feces of livestock and poultry heap grow up trapezoidal, 1 meter of heap top width, heap bottom
Wide 2 meters, 0.8~1 meter of heap high summer and autumn, 1.2~1.4 meters of winter-spring season depending on heap length is according to place, per heap volume 5 sides are no less than；
（5）The control of turning time:Kept for three days when 20~30 centimetres of depths temperature of charge rise to 55 DEG C, then turn over and smash one
It is secondary, after heap temperature rises to again 55 DEG C, kept for three days, then turn over and smash once, carry out turning over smashing according to heap tender feeling condition afterwards, in temperature
The period of liter does not turn over and smashes, and temperature stops rising then to turn over smashing, and the step for repetition, when temperature is increased beyond 70 DEG C, need to enter immediately
Row is turned over to be smash, and by cooling suppression of the high temperature to growth of microorganism is eliminated, and so as to promote the activity of a large amount of mesophilic bacterias, accelerates compost
Process；
（6）Turning is required：Turn over and to accomplish during pile tune, even, broken, it is exactly that original top section in pile is transferred to earth's surface to become to adjust
Underclad portion, and underclad portion is transferred to top layer and is changed into top section, to reach the purpose of abundant fermentation；Even is exactly to raw material in pile
The also uneven part of mixing, carries out again mixing work；Broken is exactly that the massive zymolysis thing to being formed during the fermentation is carried out
Broken work；
（7）The addition of object bacteria：After fermentation the phase, i.e., when fermentation starts cooling, add Bacillus mucilaginosus single strain bacterium
Agent, is 1 kilogram per square object material addition；
（8）Fermentation termination is controlled：When heap temperature is identical with outdoor temperature, moisture is down to less than 30%, that is, complete biological organic fertilizer
Whole fermentation production process, whole cycle is usually 15~20 days, 25~30 days time in winter；
（9）Fermented quality is controlled：Crushed after the completion of fermentation, sieved, detected, weighed and packed, bio-organic fertilizer product
Production is completed, and bio-organic fertilizer organic matter requires to reach 45%~55%, N, P, K total nutrient 5%, and living bacteria count ＞ 0.2 ×
108Individual/g, excrement colibacillus group number is negative, induced worm egg death rate 100%.
2. application of the biological organic fertilizer described in claim 1 in terms of vegetable disease-resistant effect of increasing production is improved.
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| CN103232944A (en) * | 2013-04-25 | 2013-08-07 | 南京林业大学 | Microorganism bacterium agent for straw and excrement mixed composting |
| CN103289930A (en) * | 2013-06-05 | 2013-09-11 | 中国海洋大学生物工程开发有限公司 | Microbial rotten bacterium agent as well as preparation method and application thereof |
| CN103787729A (en) * | 2013-09-13 | 2014-05-14 | 保康丰源肥业有限责任公司 | Organic fertilizer and preparation method thereof |
| CN103497899A (en) * | 2013-09-29 | 2014-01-08 | 北京农学院 | Biologically-fermented organic fertilizer and preparation method thereof |
| CN103508760A (en) * | 2013-10-10 | 2014-01-15 | 宣城市南阳生物科技有限公司 | Method for utilizing chicken manure to produce organic fertilizer |
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