CN110511892A - A kind of organic fertilizer fermentation microbial inoculum and its application - Google Patents

A kind of organic fertilizer fermentation microbial inoculum and its application Download PDF

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Publication number
CN110511892A
CN110511892A CN201910790412.2A CN201910790412A CN110511892A CN 110511892 A CN110511892 A CN 110511892A CN 201910790412 A CN201910790412 A CN 201910790412A CN 110511892 A CN110511892 A CN 110511892A
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parts
organic fertilizer
fermentation
fermenting agent
microbial inoculum
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Inventor
王逊
韦宗海
陆海英
韦青华
伍雄斌
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Guangxi Fufeng Agriculture And Animal Husbandry Group Co Ltd
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Guangxi Fufeng Agriculture And Animal Husbandry Group Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442Chitinase (3.2.1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014Chitinase (3.2.1.14)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Fertilizers (AREA)
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Abstract

The invention discloses a kind of organic fertilizer fermentation microbial inoculum and its application, the fermenting agent is made of the strain of following parts by weight: 5-10 parts of bacillus subtilis, 3-5 parts of actinomyces, 8-15 parts of Thermophilic Bacteria, 3-5 parts of aspergillus oryzae, 3-5 parts of koning trichoderma, 1-3 parts of Sphingol single-cell, 0.5-1.5 parts of vibrio harveyi and 0.5-1.5 parts of chitinase.Fermenting agent is applied to production organic fertilizer, is to be inoculated in fermenting agent in the organic fertilizer raw material that fowl and animal excrement, stalk, sludge, the dregs of a decoction are constituted with the 0.01-0.05% for organic fertilizer weight, is uniformly mixed and carries out heap fermentation, to get organic fertilizer after fermentation 7-15 days.The organic fertilizer of fermenting agent production provided by the invention can not only shorten fermentation time, and the nutrient of activating soil promotes plant growth, moreover it is possible to increase microbial bacteria, improve the insect pest characteristic of crops.

Description

A kind of organic fertilizer fermentation microbial inoculum and its application
Technical field
The present invention relates to organic fertilizer technical field, specifically a kind of organic fertilizer fermentation microbial inoculum and its application.
Background technique
Soil in planting process using chemical fertilizer can increasing both production and income to a certain extent, but be used for a long time chemical fertilizer can cause very More problems such as lack organic matter, soil hardening occur, reduce water holding water holding capacity, reduce the drawbacks such as soil property.And a large amount of chemical fertilizer Farmland is applied, practical efficiency is about 30%, and it is most of to flow into rivers and lakes with rainwater or agricultural drain, and cause water quality rich One of the principal element of nutrient laden pollution.Organic fertilizer can effectively improve soil physical chemistry situation and biological nature, enhance the guarantor of soil Crop quality and yield can be improved on the high soil of the content of organic matter in water, fertilizer conservation, feeder capability.
Organic fertilizer not only influences crop yield, also there is certain influence on soil physico-chemical property.By to alkaline land soil Analysis of physical and chemical property, in conjunction with Different Crop kind, rational proportion N P and K, microelement in appropriate supplement, while being added beneficial Microbial strains improve rhizosphere, and culture fertility improves soil aggregate structure, improves alkaline land soil, inhibit disease Opportunistic pathogen breeding and the erosion to crop root, promote crop root growth, improve seed germination rate and survival rate of seedlings, further increase Crop survival rate, increases soil fertility, and finally improves fertilizer utilization.Present most organic fertilizer is all by with special effect Can microbial fermentation made of, containing a large amount of beneficial microbes, after being manured into soil, can nutrient in activating soil, improve plant The nutrient environment of object, or generate active material stimulation plant growth, inhibit pest and disease damage.
Therefore, studying a kind of efficient organic fertilizer microbial inoculum is Developing green agriculture, the demand of the ecological agriculture, to promotion China's agricultural is fast-developing to play great meaning.
The disclosure of background above technology contents is only used for auxiliary and understands inventive concept and technical solution of the invention, not The prior art for necessarily belonging to present patent application shows above content in the applying date of present patent application in no tangible proof In the case where having disclosed, above-mentioned background technique should not be taken to the novelty and creativeness of evaluation the application.
Summary of the invention
It is an object of that present invention to provide a kind of organic fertilizer fermentation microbial inoculum and its utilize the organic fertilizer of fermenting agent production. The organic fertilizer of the fermenting agent production of offer can not only shorten fermentation time, and the nutrient of activating soil promotes plant growth, also It can increase microbial bacteria, improve the insect pest characteristic of crops.
In order to achieve the goal above, The technical solution adopted by the invention is as follows:
The present invention provides a kind of organic fertilizer fermentation microbial inoculum, and the fermenting agent is made of the strain of following parts by weight: withered grass bud 5-10 parts of spore bacillus, 3-5 parts of actinomyces, 8-15 parts of Thermophilic Bacteria, 3-5 parts of aspergillus oryzae, 3-5 parts of koning trichoderma, Sphingol single-cell 1-3 parts, 0.5-1.5 parts of vibrio harveyi and 0.5-1.5 parts of chitinase.
Preferably, the fermenting agent is made of the strain of following parts by weight: 7 parts of bacillus subtilis, actinomyces 4 Part, 12 parts of Thermophilic Bacteria, 3.5 parts of aspergillus oryzae, 4.5 parts of koning trichoderma, 2 parts of Sphingol single-cell, 0.8 part of vibrio harveyi and chitin 1.2 parts of enzyme.
Another object of the present invention is to provide the applications of the organic fertilizer fermentation microbial inoculum: the microbial inoculum has for producing Machine fertilizer, the additive amount of microbial inoculum are the 0.01-0.05% of organic fertilizer weight.
Another object of the present invention also provides the method for the organic fertilizer of fermenting agent production, include the following steps: by Fermenting agent is inoculated in the organic fertilizer raw material that fowl and animal excrement, stalk, sludge, the dregs of a decoction are constituted, and is uniformly mixed and is carried out heap fermentation, hair To get organic fertilizer after ferment 7-15 days.
Preferably, fowl and animal excrement in the organic fertilizer raw material, stalk, sludge, the dregs of a decoction weight ratio be 50-100:30-50: 20-40:5-10。
Preferably, it is first sprayed with biogas slurry so that the water content of organic fertilizer raw material is 30-50% before the fermentation.
Compared with prior art, it advantages of the present invention and has the beneficial effect that
1, fermenting agent provided by the invention can heat production during growth and breeding, temperature reaches a certain level Thermophilic Bacteria and starts to make With making material be maintained at the condition of high temperature, efficiently kill disinfect pathogen, parasitic ovum and weed seed, strain metabolite include Protease, cellulase etc. have protein, cellulose and macromolecular in the effective ingredient of plant growth and decomposition feces of livestock and poultry Machine object decomposes material further decomposed, it is decomposed after organic manure in remaining bacillus subtilis, actinomyces, crust The effects of plain enzyme etc. is applied to field together with organic fertilizer, can play antibacterial, insect prevention, volume increase.
2, the present invention using fowl and animal excrement, stalk, sludge, the dregs of a decoction as organic fertilizer raw material, these raw materials it is effective at Point can mutually coordinated and cooperation, it is balanced provide growth to crops needed for various nutriments, while the dregs of a decoction have it is wide The pathogen inhibiting effect of spectrum and bactericidal effect, and sprayed during the fermentation with biogas slurry, in biogas slurry nutrition rich at Dividing has facilitation to seed sprouting, growth of seedling and drought resisting, promotes the growth of plant.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.
Embodiment 1
A kind of organic fertilizer fermentation microbial inoculum, the fermenting agent are made of the strain of following parts by weight: 8 parts of bacillus subtilis, 4 parts of actinomyces, 12 parts of Thermophilic Bacteria, 3 parts of aspergillus oryzae, 4 parts of koning trichoderma, 2.5 parts of Sphingol single-cell, 1.2 parts of vibrio harveyi and 0.8 part of chitinase.
Embodiment 2
A kind of organic fertilizer fermentation microbial inoculum, the fermenting agent are made of the strain of following parts by weight: 6 parts of bacillus subtilis, 3.5 parts of actinomyces, 10 parts of Thermophilic Bacteria, 5 parts of aspergillus oryzae, 3 parts of koning trichoderma, 1.5 parts of Sphingol single-cell, 1.2 parts of vibrio harveyi With 0.8 part of chitinase.
Embodiment 3
A kind of organic fertilizer fermentation microbial inoculum, the fermenting agent are made of the strain of following parts by weight: 7 parts of bacillus subtilis, 4.5 parts of actinomyces, 12 parts of Thermophilic Bacteria, 4.5 parts of aspergillus oryzae, 5 parts of koning trichoderma, 2 parts of Sphingol single-cell, 1.0 parts of vibrio harveyi With 1.2 parts of chitinase.
Embodiment 4
A kind of organic fertilizer fermentation microbial inoculum, the fermenting agent are made of the strain of following parts by weight: 10 parts of bacillus subtilis, 3 parts of actinomyces, 13 parts of Thermophilic Bacteria, 3.5 parts of aspergillus oryzae, 4 parts of koning trichoderma, 1.5 parts of Sphingol single-cell, 0.8 part of vibrio harveyi With 1 part of chitinase.
Embodiment 5
A kind of organic fertilizer fermentation microbial inoculum, the fermenting agent are made of the strain of following parts by weight: 8 parts of bacillus subtilis, 4 parts of actinomyces, 11 parts of Thermophilic Bacteria, 4 parts of aspergillus oryzae, 5 parts of koning trichoderma, 2 parts of Sphingol single-cell, 1.2 parts of vibrio harveyi and first 0.8 part of shell element enzyme.
Application Example
The fermenting agent that above-described embodiment 1-5 is obtained is inoculated in fowl and animal excrement, stalk, dirt with the 0.03% of organic fertilizer weight It in the organic fertilizer raw material that mud, the dregs of a decoction are constituted, is uniformly mixed and carries out heap fermentation, to get organic fertilizer after fermentation 10 days, before fermentation first It is sprayed with biogas slurry so that the water content of organic fertilizer raw material is 45%.Fowl and animal excrement, stalk, sludge, the dregs of a decoction in the organic fertilizer raw material Weight ratio be 80:50:40:10.Referring to organic fertilizer standard (NY 525-2012) relevant regulations to embodiment 6-12 and right The organic fertilizer of ratio (agriculture Citroen zx bio-bacterial manure leavening) carries out organic matter, total nutrient and the measurement beneficial to bacterial content.As a result such as Following table:
Table 1: the effective constituent determination result of the organic fertilizer of fermenting agent fermentation of the present invention
Product Organic matter (%) Total nutrient (N, P2O5,K2O%) Beneficial bacterium (cfu, hundred million/g)
Embodiment 1 53.6 13.1 10.9
Embodiment 2 54.1 14.5 10.1
Embodiment 3 52.8 12.6 9.2
Embodiment 4 51.4 13.5 9.6
Embodiment 5 55.2 13.7 10.4
Comparative example 48.6 6.3 1.2
It learns from the above, using fermenting agent of the present invention compared with commercial product, the organic fertilizer fertilizer efficiency of preparation is higher.
The above content is combine it is specific/further detailed description of the invention for preferred embodiment, cannot Assert that specific implementation of the invention is only limited to these instructions.General technical staff of the technical field of the invention is come It says, without departing from the inventive concept of the premise, some replacements or modifications can also be made to the embodiment that these have been described, And these substitutions or variant are regarded as belonging to the scope of protection of the present invention.

Claims (6)

1. a kind of organic fertilizer fermentation microbial inoculum, it is characterised in that: the fermenting agent is made of the strain of following parts by weight: withered grass 5-10 parts of bacillus, 3-5 parts of actinomyces, 8-15 parts of Thermophilic Bacteria, 3-5 parts of aspergillus oryzae, 3-5 parts of koning trichoderma, Sphingomonas 1-3 parts of bacterium, 0.5-1.5 parts of vibrio harveyi and 0.5-1.5 parts of chitinase.
2. organic fertilizer fermentation microbial inoculum according to claim 1, it is characterised in that: the fermenting agent is by following parts by weight Strain composition: 7 parts of bacillus subtilis, 4 parts of actinomyces, 12 parts of Thermophilic Bacteria, 3.5 parts of aspergillus oryzae, 4.5 parts of koning trichoderma, sheath ammonia 2 parts of alcohol monad, 0.8 part of vibrio harveyi and 1.2 parts of chitinase.
3. the application of organic fertilizer fermentation microbial inoculum as claimed in claim 1 or 2, it is characterised in that: the microbial inoculum has for producing Machine fertilizer, the additive amount of microbial inoculum are the 0.01-0.05% of organic fertilizer weight.
4. the method for the organic fertilizer of fermenting agent production as described in claim 1, characterized by the following steps: will send out Yeast-like fungi agent is inoculated in the organic fertilizer raw material that fowl and animal excrement, stalk, sludge, the dregs of a decoction are constituted, and is uniformly mixed and is carried out heap fermentation, fermentation To get organic fertilizer after 7-15 days.
5. the method for the organic fertilizer of fermenting agent production according to claim 4, it is characterised in that: in the organic fertilizer raw material Fowl and animal excrement, stalk, sludge, the dregs of a decoction weight ratio be 50-100:30-50:20-40:5-10.
6. the method for the organic fertilizer of fermenting agent production according to claim 4, it is characterised in that: first used before the fermentation Biogas slurry is sprayed so that the water content of organic fertilizer raw material is 30-50%.
CN201910790412.2A 2019-08-26 2019-08-26 A kind of organic fertilizer fermentation microbial inoculum and its application Pending CN110511892A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113603540A (en) * 2021-08-25 2021-11-05 广西益楠山农牧科技有限公司 Organic fertilizer additive, organic fertilizer and preparation method thereof
CN115894097A (en) * 2022-12-07 2023-04-04 御兴堂(北京)生物科技有限责任公司 Compound oligosaccharide microbial inoculum for treating livestock and poultry manure and application thereof, and organic fertilizer and preparation method and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN104030746A (en) * 2014-06-24 2014-09-10 湖南豫园生物科技有限公司 Organic material decomposition agent, biological organic fertilizer containing tobacco wastes, and production method and application method of biological organic fertilizer
CN105524870A (en) * 2016-01-20 2016-04-27 杭州富阳高博信息技术服务有限公司 Environmentally-friendly preparation product capable of promoting straw degradation
CN108624539A (en) * 2018-06-08 2018-10-09 河北木美土里科技有限公司 A kind of stalk and cow dung superhigh temperature decomposing agent and its preparation and application

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102531766A (en) * 2011-09-15 2012-07-04 北京世纪阿姆斯生物技术股份有限公司 Microbial decomposing agent and production method thereof
CN104030746A (en) * 2014-06-24 2014-09-10 湖南豫园生物科技有限公司 Organic material decomposition agent, biological organic fertilizer containing tobacco wastes, and production method and application method of biological organic fertilizer
CN105524870A (en) * 2016-01-20 2016-04-27 杭州富阳高博信息技术服务有限公司 Environmentally-friendly preparation product capable of promoting straw degradation
CN108624539A (en) * 2018-06-08 2018-10-09 河北木美土里科技有限公司 A kind of stalk and cow dung superhigh temperature decomposing agent and its preparation and application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113603540A (en) * 2021-08-25 2021-11-05 广西益楠山农牧科技有限公司 Organic fertilizer additive, organic fertilizer and preparation method thereof
CN115894097A (en) * 2022-12-07 2023-04-04 御兴堂(北京)生物科技有限责任公司 Compound oligosaccharide microbial inoculum for treating livestock and poultry manure and application thereof, and organic fertilizer and preparation method and application thereof

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