CN104542730B - A kind of multifunction activity biological insecticides and preparation method thereof - Google Patents
A kind of multifunction activity biological insecticides and preparation method thereof Download PDFInfo
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- CN104542730B CN104542730B CN201510004304.XA CN201510004304A CN104542730B CN 104542730 B CN104542730 B CN 104542730B CN 201510004304 A CN201510004304 A CN 201510004304A CN 104542730 B CN104542730 B CN 104542730B
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Abstract
The present invention relates to multifunction activity biological insecticides and preparation method thereof, can effectively it solve the problems, such as in agricultural plant production largely using soil pollution caused by high poison persistent pesticide is serious, quality of agricultural product declines, its technical solution solved is that the insecticide is made of the raw material of following percentage by weight meter:Biogas slurry 62 68%, cigarette ink 8 12%, Fructus meliae toosendan 8 12%, pumpkin seeds 4 6%, sulphur 1 2%, alum 1 2%, frutus cnidii 3 5%, radix glycyrrhizae 1.5 2.5% and active biochemical bacteria 0.8 1.2%;The cigarette ink is remaining vein after tobacco leaf rebaking factory and cigar mill's tobacco leaf baking, is ground into a section shape, 0.8 1.2cm of length;The active biochemical bacteria is active biochemical bacteria prepared by patent No. ZL200610107370.0 preparation methods, the insecticide that the present invention produces contains high activity flora, common subterranean pest-insect and ground pest and disease damage can be killed, it is environmentally safe, ecological, environmental protective, its product purpose is wide, and using effect is good, is the innovation on biological insecticides.
Description
Technical field
The present invention relates to agriculture field, particularly a kind of multifunction activity biological insecticides and preparation method thereof.
Background technology
Microbial technique is prepared in proportion of crop planting, biological pesticide, solves herding agricultural product food security, protects agriculture ring
The field such as border and raising agriculture production technology level all has broad application prospects, and developed country is in agricultural production to micro-
The application of biology is quite varied, and microbial technique is solving plant agricultural product residual caused by high poison persistent pesticide improper use
Effective way is provided in terms of the problems such as thing is exceeded, and pest and disease damage easily occurs.China produces biological agriculture using microbial technique
Medicine is started late, far behind developed country, but the needs of development and the actual production with science and technology, production scale and level
It there has been and significantly lifted.In recent years, the different fertilizer of existing more than 300 families and Pesticidal products register in the Ministry of Agriculture, production, also have
Many products are just in AsTl deposit, and total output is more than 3,500,000 tons.21 century is that a biology profound influence human society is each
In the century in field, the application development impetus of the agriculture microorganism on biological pesticide and organic fertilizer is gratifying, big promising.Mesh
Before, more than 300 plants of China's agricultural microbial preservation strain, but for various reasons, China's beneficial microorganism breeding amount is small, breeding
It is horizontal low, it is impossible to meet needs of production, research and develop cultivating excellent microorganism species, and apply it to actual production
Cheng Zhong, is the key subjects that scientific worker faces all the time.
The content of the invention
For the above situation, to solve the defect of the prior art, the purpose of the present invention is just to provide a kind of multifunction activity
Biological insecticides and preparation method thereof, can effectively solve soil caused by largely using high poison persistent pesticide in agricultural plant production
The problem of earth is seriously polluted, quality of agricultural product declines.
The technical solution that the present invention solves is that the insecticide is made of the raw material of following percentage by weight meter:Biogas slurry 62-
68%th, cigarette ink 8-12%, Fructus meliae toosendan 8-12%, pumpkin seeds 4-6%, sulphur 1-2%, alum 1-2%, frutus cnidii 3-5%, radix glycyrrhizae 1.5-
2.5% and active biochemical bacteria 0.8-1.2%;The biogas slurry is derived from the urine of provincial standardization cultivation pasture generation through supporting
Methane station fermentation after the isabelline liquid water that produces, or for cow dung, rabbit excrement, chicken manure mixed fermentation forms, be that people are widely known
The bio-organic fertilizer that has both of a kind of quick-acting and long-term effect(Known technology), the cigarette ink is tobacco leaf rebaking factory and cigarette
Remaining vein after factory's tobacco leaf baking, is ground into a section shape, length 0.8-1.2cm, the active biochemical bacteria is the patent No.
Active biochemical bacteria prepared by ZL200610107370.0 preparation methods, is specifically:Rumen fluid is extracted first, then carries out classification opportunistic pathogen
Expand bacterium culture, method is in the Rumen of livestock for directly acquiring from the healthy ruminant domestic animal stomach slaughtered or being ruminated with conduit from health
Suction out, carry out level-one biochemistry opportunistic pathogen and expand culture, i.e., be put into seed cylinder temperature be 45 DEG C, volume be 6 times of amounts of rumen fluid
Water, the opportunistic pathogen water for being made 7 times of rumen fluid opportunistic pathogen amount mix that liquid is spare, then by by the straw powder 2% in terms of mixing liquid weight by opportunistic pathogen water, beautiful
Rice flour or high-quality hay 0.5%, salt 0.1% and ammonium hydrogen carbonate 0.6%-0.8% are mixed evenly and culture medium are made, and are in pH value
7.2 ± 0.2, when temperature is 42 DEG C ± 2 DEG C, Manufactured opportunistic pathogen water is mixed into liquid inoculation in the medium, immediately with plastics fabric width
Mouth, capping, cause anaerobic environment, after sealing 2-3 days, reach filter paper disintegration, then carry out secondary biochemical and strain subculture, institute again
The secondary biochemical and strain subculture said, are that the water that the bacterium solution that the biochemical expansion training of level-one has been grown is added to 3 times of amounts of its volume are made two
Level expands bacterium solution, and it is 7.2 ± 0.2 to inoculate in PH, and temperature is in 42 DEG C ± 2 DEG C of culture medium, and culture medium with two level by expanding bacterium solution
Weight meter:Straw powder 2%, fine fodder corn flour or high-quality hay 0.5%, salt 0.1% and ammonium hydrogen carbonate 0.6-0.8% are mixed
Uniformly be made, after inoculation, sealed immediately with plastic cloth, be capped, cause anaerobic environment, after sealing 2-3 days, reach filter paper be disintegrated,
Fiber hydrolization rate is 1.5-3%, protein increase by 50%, is required active biochemical bacteria.
The method for producing insecticide, comprises the following steps:
1)By said components compatibility, biogas slurry, cigarette ink, Fructus meliae toosendan, pumpkin seeds, sulphur, alum, frutus cnidii and radix glycyrrhizae are mixed
It is even, glass jar tank is put into, active biochemical bacteria is added, obtains mixture;
2)Mixture is sealed, when 15-27 DEG C of anaerobic fermentation 48-72 is small, obtains fermentate;
3)Fermentate is filtered, the dregs of a decoction is removed, obtains liquid, is multifunction activity biological insecticides, dispenses.
Biological insecticides produced by the invention are in dark brown, containing high activity flora, can kill common subterranean pest-insect and
Ground pest and disease damage, environmentally safe, the ferment bacterium of similar Japan, EM, ten thousand fields are fermented three big beneficial bacterium brands, used in the present invention
Active bacteria is the active bacteria product of inventor's independent research, has originality, the biocidal activity produced using the present invention
Height, ecological, environmental protective, its product purpose is wide, and using effect is good, is the innovation on biological insecticides.
Embodiment
The embodiment of the present invention is described in further detail with reference to embodiments.
Embodiment 1
The insecticide is made of the raw material of following percentage by weight meter:Biogas slurry 62%, cigarette ink 12%, Fructus meliae toosendan 11%, pumpkin
Son 6%, sulphur 1%, alum 2%, frutus cnidii 3%, radix glycyrrhizae 1.8% and active biochemical bacteria 1.2%.
Embodiment 2
The insecticide is made of the raw material of following percentage by weight meter:Biogas slurry 65%, cigarette ink 10%, Fructus meliae toosendan 10%, pumpkin
Son 5%, sulphur 1.5%, alum 1.5%, frutus cnidii 4%, radix glycyrrhizae 2% and active biochemical bacteria 1%.
Embodiment 3
The insecticide is made of the raw material of following percentage by weight meter:Biogas slurry 68%, cigarette ink 8%, Fructus meliae toosendan 9%, pumpkin seeds
4%th, sulphur 2%, alum 1%, frutus cnidii 5%, radix glycyrrhizae 2.2% and active biochemical bacteria 0.8%.
Method for producing insecticide described in embodiment 1-3 with Summary preparation method, i.e.,:
1)Biogas slurry, cigarette ink, Fructus meliae toosendan, pumpkin seeds, sulphur, alum, frutus cnidii and radix glycyrrhizae are mixed, are put into glass jar tank,
Active biochemical bacteria is added, obtains mixture;
2)Mixture is sealed, when 15-27 DEG C of anaerobic fermentation 48-72 is small, obtains fermentate;
3)Fermentate is filtered, the dregs of a decoction is removed, obtains liquid.As multifunction activity biological insecticides, packing, weight is every
5 kilograms of bucket, according to the description of product, dosage is 1 barrel per acre.
It is 1 barrel of progress field trial according to dosage per acre through experiment with biological insecticides of the present invention, wherein, green vegetables, spinach
Each 2 mu of dish, Chinese cabbage, wheat, corn, make field trial by Nanyang Institute of Technology plant institute doctor in Nanyang Wancheng District, even
Continue 3 years growth periods in above-mentioned vegetables and crops, to foliage-spray 2-3 times, it is demonstrated experimentally that being killed by the use of this product as biology
Worm agent, is up to the common agricultural insect pests control such as blade face aphid, cabbage caterpillar, wheat red spider, Apopnyliaflavoviyens and cutworm
96%, and to plant without poisoning, it is also good substantially without toxic action, ecological, environmental protective Property comparison to pest natural enemy, it was demonstrated that the present invention
The liquid fermented contains active insecticidal components, which is 2 years.
The innovation of the present invention is:Discarded biogas slurry, cigarette ink and Chinese herbal medicine are sent out using active bacteria technology first
Ferment, produces efficient, environmental-friendly biological bactericide, avoids the environmental pollution of farm's biogas slurry, realizes straw biological
The ecological circulation of resource utilizes, and creates the energy conservation and environmental protection benefit of " achieving many things at one stroke ".
Compared with prior art, the present invention has the following advantages:
1. pest and disease damage preventive effect is good
In biological insecticides prepared by the present invention, the crucial active ingredients in insecticidal action are essentially from Chinese herbal medicine.
Microbial fermentation Chinese herbal medicine, by materials such as the organic acids, alcohol and enzyme produced during fermentating metabolism, dissolves the ore deposit in Chinese herbal medicine
The sterilization component such as element, decomposition, the vegetable soda in conversion Chinese herbal medicine, flavonoids, terpenes, can improve more than 30 times of drug effect, add
Tens kinds of high rhzomorphs that upper metabolism produces, can greatly improve the effect of diseases prevention insect prevention.
2. cost is low, efficient
Biological insecticides prepared by the present invention, cost is low, and compared with general chemistry pesticide, cost is only the 70% of its cost.
In addition, by the medicament fiting chemical Pesticide use, half pesticide dosage can be both reduced, drug effect can also be promoted, reduces and uses
Cost.Moreover, the technology of preparing is easy to operate, it is easy to grasp, input-output ratio is high.
It is 3. ecological friendly
The insecticide belongs to biological pesticide insecticides, and application effect is good, to other no toxic biological, no sharp aroma,
And residual quantity of the high-toxic pesticide in agricultural product can be reduced, overcomes high concentration agricultural chemical taste to be polluted caused by air ambient,
Meet ecological friendly requirement.
In short, the present invention is microorganism, biogas slurry, cigarette ink and Chinese herbal medicine connected applications, scale raising is not only solved
The pollution problem of excrement thing biogas slurry, the recycling of light industry enterprise waste is completed using cigarette ink, creates new value, it was found that one
The new way that bar agricultural wastes recycle, while meet the requirement of agricultural sustainable development, ecological, environmental protective characteristic is preferable, promotes
Have a extensive future, especially the popularization in Route watershed, biological insecticides of the present invention can kill leaf
The common agricultural pests such as face aphid, cabbage caterpillar, wheat red spider, Apopnyliaflavoviyens and cutworm, it is proposed that peasant household uses sprayer
Spray and dress seed use, and production technology of the present invention is complete, and operating method is simple, and product practice effect is good, and no sharp aroma, has
The effect of plant common pests are prevented, purposes is wide, and value is big, there is wide development prospect, can bring huge economy,
Social benefit and ecological benefits.
Claims (4)
1. a kind of multifunction activity biological insecticides, it is characterised in that the insecticide is by the raw material of following percentage by weight meter
It is made:Biogas slurry 62-68%, cigarette ink 8-12%, Fructus meliae toosendan 8-12%, pumpkin seeds 4-6%, sulphur 1-2%, alum 1-2%, frutus cnidii 3-
5%th, radix glycyrrhizae 1.5-2.5% and active biochemical bacteria 0.8-1.2%;By said components compatibility, by biogas slurry, cigarette ink, Fructus meliae toosendan, pumpkin seeds,
Sulphur, alum, frutus cnidii and radix glycyrrhizae mix, and are put into glass jar tank, add active biochemical bacteria, obtain mixture;Mixture is sealed,
When 15-27 DEG C of anaerobic fermentation 48-72 is small, fermentate is obtained;Fermentate is filtered, the dregs of a decoction is removed, obtains liquid, is multifunction activity
Biological insecticides, packing;The cigarette ink is remaining vein after tobacco leaf rebaking factory and cigar mill's tobacco leaf baking, is ground into section
Shape, length 0.8-1.2cm;The active biochemical bacteria is raw for activity prepared by patent No. ZL200610107370.0 preparation methods
Change bacterium, be specifically:Rumen fluid is extracted first, then carries out classification opportunistic pathogen and expands bacterium culture, and method is from the healthy ruminant domestic animal slaughtered
Directly acquired in stomach, carry out level-one biochemistry opportunistic pathogen and expand culture, i.e., be put into seed cylinder temperature be 45 DEG C, volume be rumen fluid
The water of 6 times of amounts, the opportunistic pathogen water for being made 7 times of rumen fluid opportunistic pathogen amount mix that liquid is spare, then by by the stalk in terms of mixing liquid weight by opportunistic pathogen water
Powder 2%, corn flour or high-quality hay 0.5%, salt 0.1% and ammonium hydrogen carbonate 0.6%-0.8% are mixed evenly and culture medium are made,
It is 7.2 ± 0.2 in pH value, when temperature is 42 DEG C ± 2 DEG C, Manufactured opportunistic pathogen water is mixed into liquid inoculation in the medium, is used immediately
Plastic cloth sealing, capping, cause anaerobic environment, after sealing 2-3 days, reach filter paper disintegration, then carry out secondary biochemical and bacterium again
Kind subculture, described secondary biochemical and strain subculture, are that the bacterium solution that the biochemical expansion training of level-one has been grown is added 3 times of amounts of its volume
Water is made two level and expands bacterium solution, and it is 7.2 ± 0.2 to inoculate in PH, and temperature is that culture medium is by with two in 42 DEG C ± 2 DEG C of culture medium
Level expands bacterium solution weight meter:Straw powder 2%, fine fodder corn flour or high-quality hay 0.5%, salt 0.1% and ammonium hydrogen carbonate 0.6-0.8%
It is mixed evenly and is made, after inoculation, sealed immediately with plastic cloth, is capped, cause anaerobic environment, after sealing 2-3 days, reaches filter
Paper disintegration, fiber hydrolization rate are 1.5-3%, protein increase by 50%, are required active biochemical bacteria.
2. multifunction activity biological insecticides according to claim 1, it is characterised in that the insecticide is by following weight
The raw material of percentages is made:Biogas slurry 62%, cigarette ink 12%, Fructus meliae toosendan 11%, pumpkin seeds 6%, sulphur 1%, alum 2%, frutus cnidii 3%,
Radix glycyrrhizae 1.8% and active biochemical bacteria 1.2%.
3. multifunction activity biological insecticides according to claim 1, it is characterised in that the insecticide is by following weight
The raw material of percentages is made:Biogas slurry 65%, cigarette ink 10%, Fructus meliae toosendan 10%, pumpkin seeds 5%, sulphur 1.5%, alum 1.5%, cnidium monnieri
Son 4%, radix glycyrrhizae 2% and active biochemical bacteria 1%.
4. multifunction activity biological insecticides according to claim 1, it is characterised in that the insecticide is by following weight
The raw material of percentages is made:It is biogas slurry 68%, cigarette ink 8%, Fructus meliae toosendan 9%, pumpkin seeds 4%, sulphur 2%, alum 1%, frutus cnidii 5%, sweet
Grass 2.2% and active biochemical bacteria 0.8%.
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CN105724450B (en) * | 2016-01-28 | 2018-08-24 | 山东十方环保能源股份有限公司 | A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry |
CN105941497A (en) * | 2016-05-13 | 2016-09-21 | 开县雄腾养殖股份合作社 | Botanical insecticide prepared from biogas slurry and traditional Chinese medicines |
CN106719934A (en) * | 2016-11-28 | 2017-05-31 | 国营全椒县棉花原种总场 | A kind of corn seed seed dressing |
CN106818909A (en) * | 2016-12-31 | 2017-06-13 | 新昌县云河虫害防治技术开发有限公司 | A kind of multifunctional insecticide agent and application thereof |
CN107950605A (en) * | 2017-11-30 | 2018-04-24 | 钟山佳欣果蔬有限责任公司 | The microcapsule formulation of citrus orchard pest control |
CN109805034A (en) * | 2019-02-27 | 2019-05-28 | 杨沾 | Degradable efficient greenbelt herbicide of one kind and preparation method thereof |
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CN1944624A (en) * | 2006-10-31 | 2007-04-11 | 南阳兴农生物技术开发有限公司 | Method for cultivating and preparing active biochemical bacteria |
CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
CN102265908B (en) * | 2010-06-04 | 2013-09-25 | 郑州大学 | Tobacco matrix composite biopesticide and preparation method thereof |
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CN101108780A (en) * | 2006-07-18 | 2008-01-23 | 郭以强 | Biological pesticide.foliage fertilizer |
CN1944624A (en) * | 2006-10-31 | 2007-04-11 | 南阳兴农生物技术开发有限公司 | Method for cultivating and preparing active biochemical bacteria |
CN102265908B (en) * | 2010-06-04 | 2013-09-25 | 郑州大学 | Tobacco matrix composite biopesticide and preparation method thereof |
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