CN105724450B - A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry - Google Patents
A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
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Abstract
The invention discloses a kind of methods for the pesticide preparing prevention bradysia odoriphaga using biogas slurry, take biogas slurry and herb liquid, complex micro organism fungicide is added, be uniformly mixed and pesticide is made, and the pesticide biogas slurry and herb liquid volume ratio are 200 235:85.The beneficial effects of the invention are as follows：The control method biogas slurry of low cost generated using kitchen garbage solves the process problem of product after garbage disposal, of low cost, turn waste into wealth.Nontoxic to people and animals, green pollution-free can replace domestic chemistry used at present, chemical industry synthesis pesticide to the not broken ring of the Nature, free from environmental pollution.The raw material sources of the present invention are extensive, and manufacture craft is simple, and energy consumption is small, and manufacturing cost is cheap.Leek solves without any pesticide residue, the edible safety problem of leek.Control effect is stablized.The control method is tested by large area, after this method, it was demonstrated that the control effect of bradysia odoriphaga is preferable, not existing visible bradysia odoriphaga harm, while can greatly increase the yield of leek.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly to the method for a kind of pesticide preparing prevention bradysia odoriphaga using biogas slurry.
Background technology
Bradysia odoriphaga is the larva of Diptera category Bradysia odoriphaga.Bradysia odoriphaga foraging territories are wider, can endanger Liliaceae, Chenopodiaceae,
30 various vegetables such as composite family, Curcurbitaceae, Cruciferae, Umbelliferae, the wherein aggrieved most serious of leek.The master that leek is planted in China
More universal, occurring area up to 90% occurs for producing region, the worm.Leek field attacked trees rate causes fragrant-flowered garlic generally 20% or more
Dish production loss is between 30%~80%.Statistics shows the probability that is infected by bradysia odoriphaga of leek after growth 2 years up to 80%
Left and right, in particular with the increase of anti-season leek and hotbed chives production, leek is increased year by year by the probability that bradysia odoriphaga infects, some ground
Area nearly reaches 100%.Bradysia odoriphaga is a kind of subterranean pest-insect of stubbornness, is the crushing pest for endangering leek growth.Bradysia odoriphaga larva master
It is clustered in underground, on the bulb of leek underground part, moth food bulb and tender shoots.After leek is aggrieved, commodity value is gently then influenced,
It is heavy then leek bulb is caused partly or wholly to rot, make the ground blade of leek it is thin and weak it is withered and yellow, wilt and rot or even dead in flakes,
Greatly compromise the interests of fragrant-flowered garlic agriculture.
A large amount of generations of bradysia odoriphaga are rapid plus its breeding, seriously affected the economic benefit of leek.It is carried to effect a radical cure bradysia odoriphaga
Root is gone out and has dialled native Disinsection method, fly net and sugar-vinegar liquid trapping method, foundation earthing, the decomposed deep placement of organic fertilizer, pick root at crop rotation
Cool soil, plant ash pull out dry, bioanalysis etc., and still, these methods have heavy workload, and efficiency is low, and input cost is high, goes out except fragrant-flowered garlic
The deficiencies of less effective of maggot.Killing bradysia odoriphaga at present, quickly and effectively method is still chemical method.The profession of national approved applications is anti-
The drug of bradysia odoriphaga is controlled mostly based on the strong phoxim of absorbability (organophosphorus insecticide) and chlopyrifos, but the harvest time of leek
It is short, not up to safety interval of pesticide pollution when leek is gathered in, and fragrant-flowered garlic agriculture has also been unable to get using the quantity and frequency of such drug
Effect control；While killing bradysia odoriphaga, soil causes its degradation speed slow the strong adsorptivity of pesticide, on the leek of some areas
Excessive pesticide residues also tend to be absorbed into vivo by leek.With the application of such pesticide, bradysia odoriphaga is also more next to the resistance of pesticide
It is stronger, force fragrant-flowered garlic agriculture go to apply more high poison in violation of rules and regulations, more effective 3911,1605 etc. pesticides form vicious circle, lead to leek agriculture
Medicine residual is exceeded.The leek after bradysia odoriphaga is killed due to the use of highly toxic pesticide, growth and development is unaffected, meanwhile, in leek
Can not also it differentiate in appearance, therefore, often this " malicious leek " is eaten in daily life, malicious leek event takes place frequently in recent years, even
Continuous malicious leek exposure event causes human body greatly to injure severe patient's even threat to life so that consumer " talks fragrant-flowered garlic color
Become ", seriously affect the economic interests of vegetable grower and the food security of consumer.
In addition the applications of pesticide have been arrived in natural environment, although having achieved the purpose that want, also bring pollution simultaneously
Problem.If environment and Pesticide Residues amount are exceeded, the mankind, other organisms or even the entire ecosystem can be caused
Harmful effect.Pesticide enters after natural ecological environment, can enter human body by approach such as respiratory tract, alimentary canal, skins.Such as
Fruit reagent toxicity is larger, it is possible to cause acute poisoning or subacute poisoning, or even cause life danger.Although some medicaments
Acute toxicity is little, but if property is stablized, is not easily decomposed, disappears after use, will remain in water, air, crops, agricultural production
In product or aquatic products, livestock products, human body is eventually entered into, slow poisoning is caused.Meanwhile pollution by pesticides can also cause other biological
Poisoning is even dead, so that destroying the ecological balance, it is therefore necessary to residual and the pollution for controlling pesticide, to reduce to human society
Influence and harm.
In addition, the yield of China's kitchen garbage is increasing in recent years, estimation account for the 50% of domestic waste total amount with
On；It endangers the appearance of the city health for directly affecting city, leads to insect pest, the plague of rats or even transmission；More seriously, part
Kitchen garbage is unprocessed and is processed into " gutter oil " into market or flows to underground farm as feed, seriously threatens people
The life and health of the people.The main processing ways of kitchen garbage include both at home and abroad at present：Burning, compost, landfill, Fodder making and detest
Aerobe fermentation.A variety of different processing methods and equipment have also been invented in people.Such as application number：201010224280.6 specially
The sharp entitled integrated conduct method using kitchen garbage gas system fertilizer.Application number：201110339482.X patent name is a kind of
Municipal organic waste combined anaerobic treatment process and equipment.Application number 201210532285.4, the recycling of patent name kitchen garbage
The method utilized, a kind of Food waste treatment method of 201210567061.7 patent name of application number etc. are disclosed rubbish
Rubbish is divided into biogas after carrying out anaerobic fermentation, and biogas slurry and biogas residue handle application respectively.
Biogas slurry is otherwise known as anaerobic fermented liquid, is the various organic matters such as people, animal excrement and agricultural crop straw, weeds through anaerobism
Liquid residue after fermentation.The conventional nutrients that crops need in biogas slurry have the multiple elements such as nitrogen, phosphorus, potassium, magnesium, calcium few
Amount trace element, active organic component have the plant hormones such as humic acid, heteroauxin, lactic acid bacteria, gibberellin, the basic element of cell division, B
Family vitamin has B1, B2, B5, B6, B11, B12, also contain there are many amino acid, protein, hydrolase and a variety of inorganic acids and
The organic molecules such as certain antibiotic, coordinative role is present in the form of available nutrient and biological pesticide mutually between these ingredients
In biogas slurry, it directly can rapidly be absorbed and kill harmful levels of pathogens and worm's ovum and promote the growth of the plants such as crops and flowers,
Therefore biogas slurry could also say that a kind of medicine fertilizer.
But these effective component contents are relatively low in biogas slurry, for concentration generally 10% or so, remaining is all free state
Water and plant fiber, silt, bacterium, clay, colloid, pesticide residue, suspended matter and the organic matter containing fishy odor, are used as
Often dosage is big when Crop fertilizer, and effect is poor, thus peasant is unwilling to make fertilizer using biogas slurry more.If biogas slurry is arranged everywhere
It puts, not only waste of resource, but also pollutes environment, it is of high cost if handled by sewage, and be difficult to handle.Crops and flower
The plants such as grass directly apply this biogas slurry, do not meet agricultural health standard, can cause the secondary pollution in farmland, can also be to growth conditions
And growing environment adversely affects, also the amount of directly this biogas slurry of application is also bigger, and this certainly will increase biogas slurry
Transportation cost and labor intensity still more use fertile season, biogas slurry that cannot be fully used, the unordered discharge of extra biogas slurry non-
The pollution and waste for causing environment need further processed in order to avoid transmission and soil pollution as fertilizer
Rear is handled to can be used.
The great attention of the leek safety in production of people is aroused in poisoning caused by a large amount of excessive problems of pesticide residue.It is many
More agriculture practitioners also recognizes the seriousness of such event, also has lofty ideals for finding environmentally friendly method, also work out
The biological agent of some prevention bradysia odoriphagas, but since cost is higher, effect does not obtain on a large scale not as good as reasons such as chemical pesticides
It is effective to promote and apply.
Therefore, how it is cheap effectively kill bradysia odoriphaga, improve the safety of food, people made to trust that edible leek is ability
One of the important topic of field technique personnel research.And the generation of kitchen garbage, current environment ability to bear is seriously tested, it will
Classify after rubbish timely processing and rationally utilize, turns waste into wealth extremely urgent.
Invention content
In order to meet the needs of administering bradysia odoriphaga and making full use of biogas slurry, biogas slurry system is utilized an embodiment of the present invention provides a kind of
The method of the pesticide of standby prevention bradysia odoriphaga.
Enterococcus faecalis CGMCC No.1.125, Trichoderma viride are CGMCC No.3.2942 and Lactobacillus rhamnosus CGMCC
No.1.12734 is purchased from Chinese microorganism strain preservation reason committee common micro-organisms center.
Carrying out the biogas slurry of processing generation using kitchen garbage in the prior art can use, but biogas slurry must be taken from just
The marsh gas fermentation pool of normal aerogenesis 3 months or more to be fermented using kitchen garbage.
In order to achieve the above-mentioned object of the invention, the present invention provides a kind of sides for the pesticide preparing prevention bradysia odoriphaga using biogas slurry
Method takes biogas slurry and herb liquid, and complex micro organism fungicide is added, and is uniformly mixed and pesticide is made, biogas slurry and herb liquid in the pesticide
Volume ratio is 200-235:85.
The complex micro organism fungicide includes enterococcus faecalis CGMCC No.1.125, Trichoderma viride CGMCC
No.3.2942 and Lactobacillus rhamnosus CGMCCNo.1.12734.
In the pesticide：The quantity of enterococcus faecalis CGMCC No.1.125 is 5.3~9.5 × 108CFU/ml, green wood
The quantity of mould CGMCC No.3.2942 is 4.0~6.0 × 108CFU/ml, Lactobacillus rhamnosus CGMCCNo.1.12734's
Quantity is 8.9~9.5 × 108 CFU /ml。
Chinese medicine in the herb liquid includes following raw medicinal material：Humulus grass, hairyvein agrimony, purslane, peach leaf, is done Fructus meliae toosendan
Tongue, oldham groundsel herb, Herba Beronicastri brunoniani and deflection evaluation.
The mass parts of each raw medicinal material are in the herb liquid：18-25 parts of humulus grass, 15-19 parts of Fructus meliae toosendan, hairyvein agrimony 8-16
Part, 28-32 parts of purslane, 4-10 parts of peach leaf, tongue 22-32 parts dry, 5-8 parts of oldham groundsel herb, 4-13 parts of Herba Beronicastri brunoniani and deflection evaluation
3-7 parts.
The mass parts of each raw medicinal material are in the herb liquid：21 parts of humulus grass, 19 parts of Fructus meliae toosendan, 8 parts of hairyvein agrimony, purslane
5 parts of 30 parts, 4 parts of peach leaf, 32 parts of dry tongue, 5 parts of oldham groundsel herb, 4 parts of Herba Beronicastri brunoniani and deflection evaluation.
The mass parts of each raw medicinal material are in the herb liquid：18 parts of humulus grass, 16 parts of Fructus meliae toosendan, 12 parts of hairyvein agrimony, dent
7 parts of amaranth 28,10 parts of peach leaf, 29 parts of dry tongue, 6 parts of oldham groundsel herb, 8 parts of Herba Beronicastri brunoniani and deflection evaluation.
The mass parts of each raw medicinal material are in the herb liquid：25 parts of humulus grass, 15 parts of Fructus meliae toosendan, 16 parts of hairyvein agrimony, dent
3 parts of 32 parts of amaranth, 8 parts of peach leaf, 22 parts of dry tongue, 8 parts of oldham groundsel herb, 13 parts of Herba Beronicastri brunoniani and deflection evaluation.
The biogas slurry is derived from the marsh gas fermentation pool to ferment using kitchen garbage of normal gas producing 3 months or more.
The preparation method of the herb liquid includes the following steps：
Humulus grass, oldham groundsel herb, Fructus meliae toosendan and hairyvein agrimony are mixed to obtain mixture one by the first step in proportion, are 70% with determining alcohol
~80% ethyl alcohol is as solvent, and using percolation, slowly diacolation, collection percolate depressurize with the speed of 0.3-0.8ml per minute
It is concentrated and dried, obtains dry cream, be ground into powder one, the quality for the ethyl alcohol being added is 3~5 times of one mass of mixture；
Purslane, peach leaf, dry tongue, Herba Beronicastri brunoniani and deflection evaluation are mixed, obtain mixture two, add by second step in proportion
Enter the distilled water relative to 9~10 times of two mass of mixture, decoct 2~4 hours, filtering obtains the first filtered fluid；Into filter residue
The distilled water relative to 3~5 times of filter residue quality is added, decocts 1~2 hour, filtering obtains the second filtered fluid；Merge the first mistake
Filtrate and the second filtered fluid are concentrated under reduced pressure, dry, obtain dry cream, are ground into powder two；
Third walks, and the powder two that powder one and second step that the first step obtains obtain is mixed, is added relative to mixed powder
The distilled water of 3~5 times of amounts of last quality, 0.5~0.8 times of honey measured, stir evenly acquisition herb liquid；
Or the preparation method of the herb liquid includes the following steps：
Step 1：It by the raw medicinal material, is mixed in the ratio, it is the 6- of raw material gross weight to add suitable water, water consumption
It 10 times, impregnates 1-2 hours；
Step 2：Soaked raw medicinal material elder generation intense fire is fried open, turns that slow boiling is continuous to be decocted, after decocting 1-2 hour, filters to obtain and filter
The water of 3-4 times of the filter residue weight is added in filter residue for liquid one and filter residue, and intense fire is fried open, and turns that slow boiling is continuous to be decocted, and decocts 0.5-1 hours
Afterwards, it filters to take filtrate and obtains filtrate two, filtrate one and filtrate two are uniformly mixed to the herb liquid of the as present invention.
The advantageous effect that technical solution provided in an embodiment of the present invention is brought is：
The control method biogas slurry of low cost generated using kitchen garbage solves the process problem of product after garbage disposal,
It is of low cost, turn waste into wealth.Biogas slurry plants effective organic fertilizer source as leek, and supply leek grows required nutrient,
Effectively raise leek yield and quality.It is nontoxic to people and animals, green pollution-free, to the not broken ring of the Nature, not dirty
Environment is contaminated, domestic chemistry used at present, chemical industry synthesis pesticide can be replaced.The raw material sources of the present invention are extensive, manufacture craft letter
Single, energy consumption is small, and manufacturing cost is cheap.Leek solves without any pesticide residue, the edible safety problem of leek.
Control effect is stablized.The control method is tested by large area, after this method, it was demonstrated that the control effect of bradysia odoriphaga
Preferably, not existing visible bradysia odoriphaga harm, while can greatly increase the yield of leek.
Specific implementation mode
Bad for current pesticide improvement bradysia odoriphaga effect, pesticide residue is high, and problem of high cost, the present invention provides a kind of
The method that the pesticide of prevention bradysia odoriphaga is prepared using biogas slurry.
The pharmacology of each Chinese medicine material medicinal material is as follows in pesticide：
Humulus grass：Alias uproots plants after their edible portions have been harvested, draws and draw rattan, cairo morningglory root or leaf, le grass, great Ye cairo morningglory root or leafs, draw dog egg, cut people rattan.Source Moraceae
The herb of humulus plants humulus grass.Nature and flavor are sweet, bitter, tremble with fear.It is clearing heat and detoxicating, inducing diuresis to remove edema.For pulmonary tuberculosis hectic fever, enterogastritis, dysentery
Disease, cold, fever, difficult urination, pyelonephritis, acute nephritis, cystitis, stone in urinary system；Carbuncle furuncle poison is controlled in external application, eczema,
Venomous snake bite.
Fructus meliae toosendan：《National Chinese herbal medicine compilation》Alias FRUCTUS TOOSENDAN, melia toosendan are real.This product is the drying and ripening of Meliaceae plant melia toosendan
Fruit.Hardship is trembled with fear；It is slightly poisonous.Return liver, small intestine, bladder warp.Easypro liver promoting qi circulation and relieving pain, expelling parasite.For chest side of body, abdominal distention, colic,
Abdominal pain due to enterositosis.
Hairyvein agrimony：《National Chinese herbal medicine compilation》Alias Agrimony, fortune silene herb with root, wolf's fang grass, goldentop fossilia dentis mastodi, Huanglong's tail, dry foot
Mattress.This product is the dry aerial parts of rosaceous plant hairyvein agrimony.It is bitter, puckery, it puts down.Converge to heart and liver channels.Astringing to arrest bleeding, preventing malaria, only
Dysentery, removing toxic substances.For hemoptysis, spit blood, metrostaxis, malaria, bloody flux, de-power internal lesion caused by overexertion, carbuncle sore tumefacting virus, under pruritus vulvae band.
Purslane：Acid is trembled with fear.Clearing heat and promoting diuresis, removing pattogenic heat from the blood and toxic material from the body.For bacillary dysentery, acute gastroenteritis, acute appendicitis,
Mastitis, hemorrhoid hemorrhage, leukorrhea；Furuncle swelling toxin, eczema, herpes zoster are controlled in external application.
Peach leaf：This product is the leaf of rosaceous plant peach.Summer and autumn harvest, fresh or dried.Hardship is put down.Clearing heat and detoxicating, desinsection stops
Itch.For malaria, carbuncle furuncle, hemorrhoid, eczema, Trichomonas vaginalis.
Dry tongue：Alias stone hair, sea sedge, sea sedge dish, green moss, Ulva lactuca L, edible seaweed.Medicine elementary source：For Ulvaceae plant Enteromorpha,
The frond of Enteromorpha, flat Enteromorpha, marginal tuber etc..《Chinese book on Chinese herbal medicine》《Dictionary of medicinal plant》It is salty；It is cold；It is slightly poisonous.Return lung；Spleen；Kidney
Through.Softening and resolving hard mass；Resolving sputum disperse accumulation；Removing toxicity for detumescence.Main goiter and tumor；Scrofula；Carbuncle swells；Boil；Dyspepsia；Malnutrition due to parasitic infestation；It is bored that gastral cavity abdomen is swollen；Nosebleed epistaxis.
Oldham groundsel herb：Alias Huang chrysanthemum lotus, cat ear, wild flax leaf, viola japonica.Medicine elementary source：For the complete of feverfew Pu root
Grass.Acrid flavour；It is bitter；It is cool in nature；Mild toxicity.It is clearing heat and detoxicating；Dampness removing；Promoting blood circulation.Main ulcerative carbuncle pyogenic infections；Urinary infection；Eczema；Traumatic injury.
【It takes passages】《Chinese book on Chinese herbal medicine》.
Herba Beronicastri brunoniani：Alias high mountain wingystem veronicastrum herb, black cohosh root, cough medicine, leaf lower red, Slight Cold medicine, it is counter carry on the back it is red.Scrophulariaceae Herba Beronicastri brunoniani,
It is used as medicine with root-like stock.It is bitter in taste, it trembles with fear.Eliminating inflammation and expelling toxin, relieving cough and reducing sputum, depressing Qi and relieving asthma, swelling and pain relieving.Cure mainly tonsillitis, stomach energy
Bitterly, cystitis, mastitis, carbuncle sore tumefacting virus, dysentery, chronic bronchitis etc..【It takes passages】《National Chinese herbal medicine compilation》.
Deflection evaluation：Alias south manyleaf meadowure rhizome and root, manyleaf meadowure rhizome and root, Radix et Rhizoma Thalictri, wintergreen barberry root.Medicine elementary source：For ranunculaceae plant
The root and rhizome of deflection evaluation.Bitter；It is cold in nature.Return stomach；Large intestine；Liver Channel.Heat-clearing and damp-drying drug；Purging intense heat and detonicating.Main damp-heat dysentery；It is yellow
Subcutaneous ulcer；Leukorrhea；Acute toothache；Red eye, swell pain；Sore swollen toxin.【It takes passages】《Chinese book on Chinese herbal medicine》.
The present invention will be described in detail below with reference to the drawings of preferred embodiments, whereby to the present invention how applied technology method
Technical problem is solved, and the realization process for reaching technique effect can fully understand and implement.
What biogas slurry was derived from sub- kitchen garbage treatment plant normal gas producing of Chongqing City's black stone 3 months or more utilizes kitchen garbage to carry out
The fermentation vat of fermentation.
Embodiment 1
A method of the pesticide preparing prevention bradysia odoriphaga using biogas slurry takes biogas slurry and herb liquid, and composite microbial bacteria is added
Agent is uniformly mixed and pesticide is made, and the pesticide biogas slurry and herb liquid volume ratio are 200:85.
Complex micro organism fungicide is enterococcus faecalis CGMCC No.1.125, Trichoderma viride CGMCC No.3.2942 and sandlwood
Sugared lactobacillus CGMCCNo.1.12734.
In pesticide：The quantity of enterococcus faecalis CGMCC No.1.125 is 5.3 × 108CFU/ml, Trichoderma viride CGMCC
The quantity of No.3.2942 is 6.0 × 108The quantity of CFU/ml, Lactobacillus rhamnosus CGMCCNo.1.12734 are 8.9 × 108
CFU /ml。
The mass parts of each raw medicinal material are in herb liquid：Humulus grass 21kg, Fructus meliae toosendan 19kg, hairyvein agrimony 8kg, purslane
30kg, peach leaf 4kg, dry tongue 32kg, oldham groundsel herb 5kg, Herba Beronicastri brunoniani 4kg and deflection evaluation 5kg.
What biogas slurry was derived from sub- kitchen garbage treatment plant normal gas producing of Chongqing City's black stone 3 months or more utilizes kitchen garbage to carry out
The marsh gas fermentation pool of fermentation.
The preparation method of herb liquid includes the following steps：
Humulus grass, oldham groundsel herb, Fructus meliae toosendan and hairyvein agrimony are mixed to obtain mixture one by the first step in proportion, are 70% with determining alcohol
~80% ethyl alcohol is as solvent, and using percolation, slowly diacolation, collection percolate are concentrated under reduced pressure with the speed of 0.5ml per minute
And it is dry, dry cream is obtained, is ground into powder one, the quality for the ethyl alcohol being added is 4 times of one mass of mixture；
Purslane, peach leaf, dry tongue, Herba Beronicastri brunoniani and deflection evaluation are mixed, obtain mixture two, add by second step in proportion
Enter the distilled water relative to 10 times of two mass of mixture, decoct 3 hours, filtering obtains the first filtered fluid；Filter residue is added opposite
It in 4 times of distilled water of filter residue quality, decocts 1 hour, filtering obtains the second filtered fluid；Merge the first filtered fluid and the second filtering
Liquid is concentrated under reduced pressure, dry, obtains dry cream, is ground into powder two；
Third walks, and the powder two that powder one and second step that the first step obtains obtain is mixed, is added relative to mixed powder
The distilled water of 4 times of amounts of last quality, 0.6 times of honey measured, stir evenly acquisition herb liquid.
Embodiment 2
A method of the pesticide preparing prevention bradysia odoriphaga using biogas slurry takes biogas slurry and herb liquid, and composite microbial bacteria is added
Agent is uniformly mixed and pesticide is made, and the pesticide biogas slurry and herb liquid volume ratio are 210:85.
Complex micro organism fungicide is enterococcus faecalis CGMCC No.1.125, Trichoderma viride is CGMCC No.3.2942 and mouse
Lee's sugar lactobacillus CGMCCNo.1.12734.
In pesticide：The quantity of enterococcus faecalis CGMCC No.1.125 is 9.5 × 108CFU/ml, Trichoderma viride CGMCC
The quantity of No.3.2942 is 5.0 × 108The quantity of CFU/ml, Lactobacillus rhamnosus CGMCCNo.1.12734 are 9 × 108
CFU /ml。
The mass parts of each raw medicinal material are in herb liquid：Humulus grass 18kg, Fructus meliae toosendan 16kg, hairyvein agrimony 12kg, purslane 28,
Peach leaf 10kg, dry tongue 29kg, oldham groundsel herb 6kg, Herba Beronicastri brunoniani 8kg and deflection evaluation 7kg.
What biogas slurry was derived from sub- kitchen garbage treatment plant normal gas producing of Chongqing City's black stone 3 months or more utilizes kitchen garbage to carry out
The fermentation vat of fermentation.
The preparation method of herb liquid includes the following steps：
Step 1：It by raw medicinal material, mixes in proportion, it is 10 times of raw material gross weight to add suitable water, water consumption, impregnates 1
Hour；
Step 2：Soaked raw medicinal material elder generation intense fire is fried open, turns that slow boiling is continuous to be decocted, after decocting 2 hours, is filtered to get filtrate
One and filter residue, the water of 4 times of the filter residue weight is added in filter residue, intense fire is fried open, and turns that slow boiling is continuous to be decocted, after decocting 0.5 hour, filtering
It takes filtrate to obtain filtrate two, filtrate one and filtrate two is uniformly mixed to the herb liquid of the as present invention.
Embodiment 3
A method of the pesticide preparing prevention bradysia odoriphaga using biogas slurry takes biogas slurry and herb liquid, and composite microbial bacteria is added
Agent is uniformly mixed and pesticide is made, and the pesticide biogas slurry and herb liquid volume ratio are 235:85.
Complex micro organism fungicide is enterococcus faecalis CGMCC No.1.125, Trichoderma viride is CGMCC No.3.2942 and mouse
Lee's sugar lactobacillus CGMCCNo.1.12734.
In pesticide：The quantity of enterococcus faecalis CGMCC No.1.125 is 9 × 108CFU/ml, Trichoderma viride CGMCC
The quantity of No.3.2942 is 4.0 × 108The quantity of CFU/ml, Lactobacillus rhamnosus CGMCCNo.1.12734 are 9.5 × 108
CFU /ml。
The mass parts of each raw medicinal material are in herb liquid：Humulus grass 25kg, Fructus meliae toosendan 15kg, hairyvein agrimony 16kg, purslane
32kg, peach leaf 8kg, dry tongue 22kg, oldham groundsel herb 8kg, Herba Beronicastri brunoniani 13kg and deflection evaluation 3kg.
What biogas slurry was derived from sub- kitchen garbage treatment plant normal gas producing of Chongqing City's black stone 3 months or more utilizes kitchen garbage to carry out
The fermentation vat of fermentation.
The preparation method of herb liquid includes the following steps：
Step 1：It by raw medicinal material, mixes in proportion, it is 6 times of raw material gross weight to add suitable water, water consumption, and it is small to impregnate 2
When；
Step 2：Soaked raw medicinal material elder generation intense fire is fried open, turns that slow boiling is continuous to be decocted, after decocting 1 hour, is filtered to get filtrate
One and filter residue, the water of 3 times of the filter residue weight is added in filter residue, intense fire is fried open, and turns that slow boiling is continuous to be decocted, and after decocting 1 hour, is filtered to take
Filtrate obtains filtrate two, and filtrate one and filtrate two are uniformly mixed to the herb liquid of the as present invention.
1, test method
In Qingdao Vegetable Base, experiment leek kind is that the only son and heir is red, and experiment sets 20 cells, and 5 processing are each to handle
4 repetitions, each plot experiment ground area are 0.5 mu.
1-3 processing is respectively the pesticide prepared using embodiment 1-3 in 5 processing,
Wherein processing 4 directly uses biogas slurry treatment for control group 1；
Processing 5 is blank control group 2, and blank is handled without any method；
Pouring mode is to be poured along root.
2, investigation content
Aggrieved strain rate investigation：With each 10 pier of 2 row of center of housing estate, statistics healthy tree and (, the lodging yellow, soft with blade tip of aggrieved strain number
Subject to).5 before dispenser and after dispenser, 12,20,30d respectively investigate 1 time, investigate 5 times altogether.
Insect population is investigated：The insect population of random searching check plot before dispenser.
Insect population of surviving is investigated：The heart digs 5 pier leek, the inside and outside maggot number of surviving of statistics bulb in the cell for last 1 investigation.
Insect population number is recorded, Revision insect recluced rate is calculated.
Investigate total strain number in attacked trees rate (%)=aggrieved strain number × 100/
Control effect (%)=（The aggrieved aggrieved strain rate of strain rate-treatment region in check plot）The aggrieved strain rate in × 100/ check plot
Insect population control effect (%)=（Check plot work borer population-treatment region work borer population）× 100/ check plot work borer population
Each processing prevention bradysia odoriphaga field control effectiveness test result referring to table 1, using rear leek per mu yield joined by each embodiment
It is shown in Table 2.
1 each processing of table prevents bradysia odoriphaga field control effectiveness test result
2 each embodiment of table uses the per mu yield of rear leek（kg）
Prevention bradysia odoriphaga effect is can be seen that by above-mentioned data clearly, and leek plant strain growth is vigorous, significantly increases
Production.Treatment effect after applying pesticides is all it is obvious that the processing insect population control effect for wherein only applying biogas slurry reaches 83.17%.But
It is the pesticide improvement bradysia odoriphaga effect highly significant of embodiment 1 and embodiment 2, it might even be possible to completely kill bradysia odoriphaga.
The per mu yield of leek is than before by the apparent volume increase of the per mu yield of bradysia odoriphaga insect pest after applying pesticides, volume increase reach 100% with
On, than 40% or more the leek per mu yield volume increase for only applying biogas slurry.It can be seen that the synergy of the pesticide of the present invention is remarkably reinforced.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of method for the pesticide preparing prevention bradysia odoriphaga using biogas slurry, which is characterized in that take biogas slurry and herb liquid, be added compound
Microbial bacterial agent is uniformly mixed and pesticide is made, and biogas slurry and herb liquid volume ratio are 200-235 in the pesticide:85；
The complex micro organism fungicide include enterococcus faecalis CGMCC No.1.125, Trichoderma viride CGMCC No.3.2942 and
Lactobacillus rhamnosus CGMCC No.1.12734；
In the pesticide：The quantity of enterococcus faecalis CGMCC No.1.125 is 5.3~9.5 × 108CFU/ml, Trichoderma viride
The quantity of CGMCC No.3.2942 is 4.0~6.0 × 108The number of CFU/ml, Lactobacillus rhamnosus CGMCC No.1.12734
Amount is 8.9~9.5 × 108CFU /ml；
The mass parts of each raw medicinal material are in the herb liquid：18-25 parts of humulus grass, 15-19 parts of Fructus meliae toosendan, 8-16 parts of hairyvein agrimony,
28-32 parts of purslane, 4-10 parts of peach leaf, tongue 22-32 parts dry, 5-8 parts of oldham groundsel herb, 4-13 parts of Herba Beronicastri brunoniani and deflection evaluation 3-7
Part.
2. according to the method described in claim 1, it is characterized in that, the mass parts of each raw medicinal material are in the herb liquid：Humulus
Grass 21 parts, 19 parts of Fructus meliae toosendan, 8 parts of hairyvein agrimony, 30 parts of purslane, 4 parts of peach leaf, 32 parts of dry tongue, 5 parts of oldham groundsel herb, 4 parts of Herba Beronicastri brunoniani and
5 parts of deflection evaluation.
3. according to the method described in claim 1, it is characterized in that, the mass parts of each raw medicinal material are in the herb liquid：Humulus
18 parts of grass, 16 parts of Fructus meliae toosendan, 12 parts of hairyvein agrimony, 28 parts of purslane, 10 parts of peach leaf, 29 parts of dry tongue, 6 parts of oldham groundsel herb, Herba Beronicastri brunoniani 8
7 parts of part and deflection evaluation.
4. according to the method described in claim 1, it is characterized in that, the mass parts of each raw medicinal material are in the herb liquid：Humulus
25 parts of grass, 15 parts of Fructus meliae toosendan, 16 parts of hairyvein agrimony, 32 parts of purslane, 8 parts of peach leaf, 22 parts of dry tongue, 8 parts of oldham groundsel herb, 13 parts of Herba Beronicastri brunoniani
With 3 parts of deflection evaluation.
5. according to the method described in claim 1, it is characterized in that, the biogas slurry is derived from the biogas of normal gas producing 3 months or more
Fermentation vat.
6. according to any methods of claim 1-5, which is characterized in that the preparation method of the herb liquid includes following step
Suddenly：
Humulus grass, oldham groundsel herb, Fructus meliae toosendan and hairyvein agrimony are mixed to obtain mixture one by the first step in proportion, with determining alcohol be 70%~
80% ethyl alcohol is as solvent, and using percolation, slowly diacolation, collection percolate, decompression are dense with the speed of 0.3-0.8ml per minute
It contracts drying, obtains dry cream, be ground into powder one, the quality for the ethyl alcohol being added is 3~5 times of one mass of mixture；
Second step mixes purslane, peach leaf, dry tongue, Herba Beronicastri brunoniani and deflection evaluation in proportion, obtains mixture two, and phase is added
It for two mass of mixture, 9~10 times of distilled water, decocts 2~4 hours, filtering obtains the first filtered fluid；It is added into filter residue
It relative to 3~5 times of distilled water of filter residue quality, decocts 1~2 hour, filtering obtains the second filtered fluid；Merge the first filtered fluid
With the second filtered fluid, it is concentrated under reduced pressure, it is dry, dry cream is obtained, powder two is ground into；
Third walks, and the powder two that powder one and second step that the first step obtains obtain is mixed, is added relative to mixed-powder matter
The distilled water of 3~5 times of amounts of amount, 0.5~0.8 times of honey measured, stir evenly acquisition herb liquid；
Or the preparation method of the herb liquid includes the following steps：
Step 1：By the raw medicinal material, mixed in the ratio, it is 6-10 times of raw material gross weight to add suitable water, water consumption,
It impregnates 1-2 hours；
Step 2：Soaked raw medicinal material elder generation intense fire is fried open, turns that slow boiling is continuous to be decocted, after decocting 1-2 hours, filters to get filtrate one
And filter residue, the water of 3-4 times of the filter residue weight is added in filter residue, intense fire is fried open, and turns that slow boiling is continuous to be decocted, after decocting 0.5-1 hours, mistake
Leaching filtrate obtains filtrate two, and filtrate one and filtrate two are uniformly mixed to the herb liquid of the as present invention.
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