CN104542730A - Multifunctional active bioinsecticide and preparation method thereof - Google Patents

Multifunctional active bioinsecticide and preparation method thereof Download PDF

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Publication number
CN104542730A
CN104542730A CN201510004304.XA CN201510004304A CN104542730A CN 104542730 A CN104542730 A CN 104542730A CN 201510004304 A CN201510004304 A CN 201510004304A CN 104542730 A CN104542730 A CN 104542730A
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liquid
bacterium
active
alum
bioinsecticide
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CN104542730B (en
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王建钦
丁传雨
韩雪梅
曹孟
崔燕
李秀清
周相林
曹明军
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Abstract

The invention relates to a multifunctional active bioinsecticide and preparation method thereof, which can effectively solve the problems that as a great amount of high-toxicity and high-residual pesticides are used in production of agricultural plants, the soil is polluted seriously, and the quality of agricultural products is reduced. According to the technical scheme, the bioinsecticide is prepared from the following raw materials in percentage by weight: 62-68% of biogas slurry, 8-12% of smoke ink, 8-12% of Fructus Meliae Toosendan, 4-6% of pumpkin seeds, 1-2% of sulfur, 1-2% of alum, 3-5% of frutus cnidii, 1.5-2.5% of radix Glycyrrhiza, and 0.8-1.2% of active biological fungus; the smoke ink is remaining veins after tobacco leaves in a tobacco redrying factory and a cigarette factory are roasted, and is grinded into sections with the length of 0.8-1.2cm; the active biological fungus is prepared by adopting a preparation method in the patent with the patent number of ZL200610107370.0, the produced bioinsecticide contains high-activity flora, can kill commonly underground and ground pests, has no environment pollution and is ecological and environmentally friendly, and the bioinsecticide is wide in applications, good in use effect, and is a creation of the bioinsecticides.

Description

A kind of multifunction activity biological insecticides and preparation method thereof
Technical field
The present invention relates to agriculture field, particularly a kind of multifunction activity biological insecticides and preparation method thereof.
Background technology
Microbial technique is at proportion of crop planting; prepared by biopesticide; solve herding agricultural product food security; the fields such as protecting agriculture environment and raising agriculture production technology level all have broad application prospects; developed country is very extensive to the application of microorganism in agricultural production; microbial technique exceeds standard solving the plant agricultural product residue that causes of high malicious persistent pesticide improper use, and damage by disease and insect problem aspect easily occurs etc. provides effective way.China utilizes microbial technique to produce biopesticide and starts late, and lag far behind developed country, but along with the development of science and technology and the needs of actual production, production scale and level there has been and significantly promote.In recent years, the fertilizer that existing more than 300 families are different and Pesticidal products are in Ministry of Agriculture's registration, production, and also have many products just at AsTl deposit, gross yield is more than 3,500,000 tons.21 century is century of a biology profound influence human society every field, and the application development impetus of agriculture microorganism on biopesticide and organic manure is gratifying, rising greatly.At present, the strain more than 300 of China's agricultural microbial preservation bacterial classification, but for various reasons, China's beneficial microorganism breeding amount is little, breeding level is low, can not meet needs of production, research and development cultivating excellent microorganism species, and apply it in actual production process, be the key subjects that scientific worker faces all the time.
Summary of the invention
For above-mentioned situation, for solving the defect of prior art, the object of the present invention is just to provide a kind of multifunction activity biological insecticides and preparation method thereof, effectively can solve during agricultural plant is produced the problem that the soil pollution using high malicious persistent pesticide to cause in a large number is serious, quality of agricultural product declines.
The technical scheme that the present invention solves is that this insecticide is made up of the raw material of following weight percent meter: natural pond liquid 62-68%, cigarette ink 8-12%, Fructus meliae toosendan 8-12%, pumpkin seeds 4-6%, sulphur 1-2%, alum 1-2%, frutus cnidii 3-5%, Radix Glycyrrhizae 1.5-2.5% and active biochemical bacteria 0.8-1.2%, described natural pond liquid is the isabelline liquid water deriving from urine generation after the fermentation of supporting methane station that provincial standardization cultivation pasture produces, or be cow dung, rabbit excrement, chicken manure mixed culture fermentation forms, a kind of quick-acting of widely knowing of people and the long-lasting bio-organic fertilizer (known technology) had both, described cigarette ink is remaining vein after tobacco leaf rebaking factory and cigar mill's tobacco leaf baking, the section of being ground into shape, length 0.8-1.2cm, described active biochemical bacteria is active biochemical bacteria prepared by patent No. ZL200610107370.0 preparation method, specifically: first extract rumen fluid, carry out the former bacterium of classification again and expand bacterium cultivation, method is sucking-off in the Rumen of livestock that directly obtains from the healthy ruminant domestic animal stomach of slaughtering or ruminate from health with conduit, carry out the biochemical former bacterium of one-level and expand cultivation, namely in seed cylinder, put into temperature is 45 DEG C, volume is the water of rumen fluid 6 times amount, it is for subsequent use that the former bacterium water making rumen fluid former bacterium 7 times amount mixes liquid, again by by the powder of straw 2% of mixing liquid weighing scale with former bacterium water, corn flour or high-quality hay 0.5%, salt 0.1% and carbonic hydroammonium 0.6%-0.8% mixing and stirring make medium, be 7.2 ± 0.2 at pH value, when temperature is 42 DEG C ± 2 DEG C, Manufactured former bacterium water is mixed liquid inoculation in the medium, seal with Polypropylence Sheet immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach filter paper disintegration, and then carry out secondary biochemical and bacterial classification subculture, said secondary biochemical and bacterial classification subculture, that the water bacterium liquid that one-level biochemistry expansion training has been grown being added its volume 3 times amount makes secondary expansion bacterium liquid, inoculating at PH is 7.2 ± 0.2, temperature is in the medium of 42 DEG C ± 2 DEG C, medium is by expand bacterium liquid weighing scale with secondary: straw powder 2%, fine fodder corn flour or high-quality hay 0.5%, salt 0.1% and carbonic hydroammonium 0.6-0.8% mixing and stirring are made, after inoculation, seal with Polypropylence Sheet immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach filter paper disintegration, fiber hydrolization rate is 1.5-3%, protein increases by 50%, be required active biochemical bacteria.
This method for producing insecticide, comprises the following steps:
1) by said components compatibility, by natural pond liquid, cigarette ink, Fructus meliae toosendan, pumpkin seeds, sulphur, alum, frutus cnidii and Radix Glycyrrhizae mixing, put into glass jar tank, add active biochemical bacteria, obtain mixture;
2) sealed by mixture, 15-27 DEG C of anaerobic fermentation 48-72 hour, obtains fermentation product;
3) filtered by fermentation product, the removing dregs of a decoction, obtain liquid, are multifunction activity biological insecticides, packing.
The biological insecticides that the present invention produces are pitchy, containing high activity flora, common soil insect and ground damage by disease and insect can be killed, environmentally safe, the ferment bacterium of similar Japan, EM, ten thousand field fermentation three large beneficial bacterium brands, the present invention's active bacteria used is the active bacteria product of inventor's independent research, has originality, and the biocidal activity using the present invention to produce is high, ecological, environmental protective, its product purpose is wide, and result of use is good, is the innovation on biological insecticides.
Embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.
Embodiment 1
This insecticide is made up of the raw material of following weight percent meter: natural pond liquid 62%, cigarette ink 12%, Fructus meliae toosendan 11%, pumpkin seeds 6%, sulphur 1%, alum 2%, frutus cnidii 3%, Radix Glycyrrhizae 1.8% and active biochemical bacteria 1.2%.
Embodiment 2
This insecticide is made up of the raw material of following weight percent meter: natural pond liquid 65%, cigarette ink 10%, Fructus meliae toosendan 10%, pumpkin seeds 5%, sulphur 1.5%, alum 1.5%, frutus cnidii 4%, Radix Glycyrrhizae 2% and active biochemical bacteria 1%.
Embodiment 3
This insecticide is made up of the raw material of following weight percent meter: natural pond liquid 68%, cigarette ink 8%, Fructus meliae toosendan 9%, pumpkin seeds 4%, sulphur 2%, alum 1%, frutus cnidii 5%, Radix Glycyrrhizae 2.2% and active biochemical bacteria 0.8%.
Method for producing insecticide described in embodiment 1-3 with the preparation method of summary of the invention part, that is:
1) by natural pond liquid, cigarette ink, Fructus meliae toosendan, pumpkin seeds, sulphur, alum, frutus cnidii and Radix Glycyrrhizae mixing, put into glass jar tank, add active biochemical bacteria, obtain mixture;
2) sealed by mixture, 15-27 DEG C of anaerobic fermentation 48-72 hour, obtains fermentation product;
3) filtered by fermentation product, the removing dregs of a decoction, obtain liquid.Be multifunction activity biological insecticides, packing, weight is 5 kilograms every barrel, is 1 barrel according to the description of product every mu consumption.
Use biological insecticides of the present invention, through test, be 1 barrel according to every mu of consumption and carry out field trial, wherein, green vegetables, spinach, Chinese cabbage, wheat, each 2 mu of corn, field trial is made in Wancheng District, Nanyang by plant institute of Nanyang Institute of Technology doctor, the continuous 3 years vegetative period above-mentioned vegetables and crops, to foliage-spray 2-3 time, experiment proves, with this product as biological insecticides, to blade face aphid, cabbage caterpillar, wheat red spider, the common agricultural insect pests control such as Apopnyliaflavoviyens and cutworm is all up to 96%, and to plant without poisoning, to pest natural enemy also substantially without toxic action, ecological, environmental protective Property comparison is good, prove that the liquid that the present invention ferments contains active insecticidal components, this medicament term of validity is 2 years.
Innovation of the present invention is: adopt active bacteria technology to ferment to discarded natural pond liquid, cigarette ink and Chinese herbal medicine first, produce efficient, eco-friendly biological bactericide, avoid the environmental pollution of plant's natural pond liquid, the ecological circulation achieving straw biological resource utilizes, and creates the energy conservation and environmental protection benefit of " achieving many things at one stroke ".
The present invention compared with prior art, has the following advantages:
1. damage by disease and insect preventive effect is good
In biological insecticides prepared by the present invention, the crucial active ingredients in insecticidal action is mainly from Chinese herbal medicine.Fermentable Chinese herbal medicine, rely on the materials such as organic acid, alcohol and the enzyme produced in fermentating metabolism process, dissolve the ore deposit element in Chinese herbal medicine, decomposition, the sterilization component such as vegetable alkaloid, flavonoids, terpenes transformed in Chinese herbal medicine, drug effect more than 30 times can be improved, add and greatly can improve the effect of diseases prevention insect protected by tens kinds of high rhzomorphs that metabolism produces.
2. cost is low, and efficiency is high
Biological insecticides prepared by the present invention, cost is low, and compared with general chemistry agricultural chemicals, cost is only 70% of its cost.In addition, by this medicament fiting chemical Pesticide use, both can reduce half pesticide dosage, can also drug effect be promoted, reduce use cost.And this technology of preparing is simple to operate, be easy to grasp, input-output ratio is high.
3. ecological friendly
This insecticide belongs to biopesticide insecticides, and application effect is good, no toxic biological to other, without sharp aroma, and the residual quantity of high-toxic pesticide in agricultural product can be reduced, overcome the pollution that high concentration agricultural chemical taste causes air ambient, meet ecological friendly requirement.
In a word, the present invention is microorganism, natural pond liquid, cigarette ink and Chinese herbal medicine connected applications, not only solve the pollution problem of scale raising excrement thing natural pond liquid, cigarette ink is used to complete the recycling of light industry enterprise refuse, create the value made new advances, find the new way of an agricultural wastes recycling, meet the requirement of agricultural sustainable development simultaneously, ecological, environmental protective characteristic is better, popularizing application prospect is wide, especially in the popularization of Route watershed, biological insecticides of the present invention can kill blade face aphid, cabbage caterpillar, wheat red spider, the common pest such as Apopnyliaflavoviyens and cutworm, suggestion peasant household adopts sprayer to spray and dress seed use, production technology of the present invention is complete, method of operating is simple, product practice effect is good, without sharp aroma, there is effect of plant common pests control, purposes is wide, be worth large, there is wide development prospect, huge economy can be brought, social benefit and ecological benefits.

Claims (5)

1. multifunction activity biological insecticides, it is characterized in that, this insecticide is made up of the raw material of following weight percent meter: natural pond liquid 62-68%, cigarette ink 8-12%, Fructus meliae toosendan 8-12%, pumpkin seeds 4-6%, sulphur 1-2%, alum 1-2%, frutus cnidii 3-5%, Radix Glycyrrhizae 1.5-2.5% and active biochemical bacteria 0.8-1.2%, described cigarette ink is remaining vein after tobacco leaf rebaking factory and cigar mill's tobacco leaf baking, the section of being ground into shape, length 0.8-1.2cm, described active biochemical bacteria is active biochemical bacteria prepared by patent No. ZL200610107370.0 preparation method, specifically: first extract rumen fluid, carry out the former bacterium of classification again and expand bacterium cultivation, method is sucking-off in the Rumen of livestock that directly obtains from the healthy ruminant domestic animal stomach of slaughtering or ruminate from health with conduit, carry out the biochemical former bacterium of one-level and expand cultivation, namely in seed cylinder, put into temperature is 45 DEG C, volume is the water of rumen fluid 6 times amount, it is for subsequent use that the former bacterium water making rumen fluid former bacterium 7 times amount mixes liquid, again by by the powder of straw 2% of mixing liquid weighing scale with former bacterium water, corn flour or high-quality hay 0.5%, salt 0.1% and carbonic hydroammonium 0.6%-0.8% mixing and stirring make medium, be 7.2 ± 0.2 at pH value, when temperature is 42 DEG C ± 2 DEG C, Manufactured former bacterium water is mixed liquid inoculation in the medium, seal with Polypropylence Sheet immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach filter paper disintegration, and then carry out secondary biochemical and bacterial classification subculture, said secondary biochemical and bacterial classification subculture, that the water bacterium liquid that one-level biochemistry expansion training has been grown being added its volume 3 times amount makes secondary expansion bacterium liquid, inoculating at PH is 7.2 ± 0.2, temperature is in the medium of 42 DEG C ± 2 DEG C, medium is by expand bacterium liquid weighing scale with secondary: straw powder 2%, fine fodder corn flour or high-quality hay 0.5%, salt 0.1% and carbonic hydroammonium 0.6-0.8% mixing and stirring are made, after inoculation, seal with Polypropylence Sheet immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach filter paper disintegration, fiber hydrolization rate is 1.5-3%, protein increases by 50%, be required active biochemical bacteria.
2. multifunction activity biological insecticides according to claim 1, it is characterized in that, this insecticide is made up of the raw material of following weight percent meter: natural pond liquid 62%, cigarette ink 12%, Fructus meliae toosendan 11%, pumpkin seeds 6%, sulphur 1%, alum 2%, frutus cnidii 3%, Radix Glycyrrhizae 1.8% and active biochemical bacteria 1.2%.
3. multifunction activity biological insecticides according to claim 1, it is characterized in that, this insecticide is made up of the raw material of following weight percent meter: natural pond liquid 65%, cigarette ink 10%, Fructus meliae toosendan 10%, pumpkin seeds 5%, sulphur 1.5%, alum 1.5%, frutus cnidii 4%, Radix Glycyrrhizae 2% and active biochemical bacteria 1%.
4. multifunction activity biological insecticides according to claim 1, it is characterized in that, this insecticide is made up of the raw material of following weight percent meter: natural pond liquid 68%, cigarette ink 8%, Fructus meliae toosendan 9%, pumpkin seeds 4%, sulphur 2%, alum 1%, frutus cnidii 5%, Radix Glycyrrhizae 2.2% and active biochemical bacteria 0.8%.
5. claim 1 or the multifunction activity process for preparing bio-insecticide described in any one of 2-4, is characterized in that, comprise the following steps:
1) by said components compatibility, by natural pond liquid, cigarette ink, Fructus meliae toosendan, pumpkin seeds, sulphur, alum, frutus cnidii and Radix Glycyrrhizae mixing, put into glass jar tank, add active biochemical bacteria, obtain mixture;
2) sealed by mixture, 15-27 DEG C of anaerobic fermentation 48-72 hour, obtains fermentation product;
3) filtered by fermentation product, the removing dregs of a decoction, obtain liquid, are multifunction activity biological insecticides, packing.
CN201510004304.XA 2015-01-06 2015-01-06 A kind of multifunction activity biological insecticides and preparation method thereof Active CN104542730B (en)

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CN105724450A (en) * 2016-01-28 2016-07-06 山东十方环保能源股份有限公司 Method for preparing pesticide capable of preventing and treating Chinese chive maggots from biogas slurry
CN105941497A (en) * 2016-05-13 2016-09-21 开县雄腾养殖股份合作社 Botanical insecticide prepared from biogas slurry and traditional Chinese medicines
CN106719934A (en) * 2016-11-28 2017-05-31 国营全椒县棉花原种总场 A kind of corn seed seed dressing
CN106818909A (en) * 2016-12-31 2017-06-13 新昌县云河虫害防治技术开发有限公司 A kind of multifunctional insecticide agent and application thereof
CN107950605A (en) * 2017-11-30 2018-04-24 钟山佳欣果蔬有限责任公司 The microcapsule formulation of citrus orchard pest control
CN109805034A (en) * 2019-02-27 2019-05-28 杨沾 Degradable efficient greenbelt herbicide of one kind and preparation method thereof

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105724450A (en) * 2016-01-28 2016-07-06 山东十方环保能源股份有限公司 Method for preparing pesticide capable of preventing and treating Chinese chive maggots from biogas slurry
CN105724450B (en) * 2016-01-28 2018-08-24 山东十方环保能源股份有限公司 A method of preparing the pesticide of prevention bradysia odoriphaga using biogas slurry
CN105941497A (en) * 2016-05-13 2016-09-21 开县雄腾养殖股份合作社 Botanical insecticide prepared from biogas slurry and traditional Chinese medicines
CN106719934A (en) * 2016-11-28 2017-05-31 国营全椒县棉花原种总场 A kind of corn seed seed dressing
CN106818909A (en) * 2016-12-31 2017-06-13 新昌县云河虫害防治技术开发有限公司 A kind of multifunctional insecticide agent and application thereof
CN107950605A (en) * 2017-11-30 2018-04-24 钟山佳欣果蔬有限责任公司 The microcapsule formulation of citrus orchard pest control
CN109805034A (en) * 2019-02-27 2019-05-28 杨沾 Degradable efficient greenbelt herbicide of one kind and preparation method thereof

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