CN1944624A

CN1944624A - Method for cultivating and preparing active biochemical bacteria

Info

Publication number: CN1944624A
Application number: CN 200610107370
Authority: CN
Inventors: 曹明军; 陈万朝; 曹爽
Original assignee: XINGNONG BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd NANYANG
Current assignee: XINGNONG BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd NANYANG
Priority date: 2006-10-31
Filing date: 2006-10-31
Publication date: 2007-04-11
Anticipated expiration: 2026-10-31
Also published as: CN100523168C

Abstract

The present invention is process of cultivating and preparing active biochemical bacteria for degrading cellulose, hemicellulose and lignin biochemically to raise the content of stalk coarse protein and amino acids. The process of cultivating and preparing active biochemical bacteria includes the following steps: adding water to rumen content or rumen juice collected from ruminant; inoculating to the culture medium comprising table salt, licorice powder, ammonium bicarbonate, etc; the first anaerobic fermentation to proliferate; adding water and culture medium and the second fermentation to obtain the active biochemical bacteria liquid product. The active biochemical bacteria liquid product has wide application foreground.

Description

The cultivation preparation method of active biochemical bacteria

One, technical field

The present invention relates to a kind of cultivation preparation method that the high-efficiency activated biochemical bacteria of multiple efficacies is arranged.

Two, background technology

The agricultural microorganism has broad application prospects in fields such as proportion of crop planting industry, livestock and poultry breeding industry, culture fishery, processing of farm products, agriculture environmental protection and agriculture production technologies.Developed country's using microbe technology in agriculture production is very extensive at present; from the application development of past single culture to the high reactivity flora; be widely used in and produce biological organic fertilizer, biological pesticide and biological fodder; be used for agricultural products fresh-keeping or be used for environment protection (sewage rubbish processing), as enzymatic microorganism, EM, ten thousand fields of Japan three famous brands of fermenting.The strain more than 3000 of China's present agriculture microorganism storehouse preservation bacterial classification, and beneficial microorganism 2003 statistics that is used for bio-feritlizer kind more than 80, the bacterial classification kind that is particularly useful for fertiliser production is few, the bacterium amount is few.China utilizes the microorganisms producing bio-organic fertilizer just to begin starting, and is also very backward.Along with development of science and technology and production actual needs, have 300 tame biological organic fertilizer products in Ministry of Agriculture's registration, production by the end of the year ends 2003, also have many registrations of declaring, ultimate production surpasses 3,500,000 tons, is 8 times of nineteen ninety-five.Find out that from above the application development impetus of agriculture microorganism on organic fertilizer is gratifying, the situation is pressing, rising greatly.21 century is biology century of profound influence human society every field more.But, for various reasons, the present beneficial microorganism breeding amount of China is little, can not satisfy the actual needs in the production, bacterial classification, multi-functional good microorganism species are cultivated in research and development, be the key subjects that China scientific and technological circle face, it is the problem that solves of making great efforts that beneficial microorganism is cultivated breeding.

Three, summary of the invention

At above situation, the present invention's purpose just provides a kind of cultivation preparation method of high-efficiency activated biochemical bacteria, to solve in the production beneficial microorganism is needed, the biochemical degradation Mierocrystalline cellulose, hemicellulose, xylogen, increase straw crude protein matter, the problem of aminoacids content, the technical scheme of its solution is: ruminant domestic animal rumen content or the gastric juice that will adopt add entry, be inoculated in by salt, licorice powder, carry out one-level biochemistry in the substratum of compositions such as bicarbonate of ammonia, under suitable temperature and pH value, carry out anaerobically fermenting, expand add again after numerous entry and substratum with the biochemical identical condition of one-level under carry out secondary biochemical and make active biochemical bacteria (liquid) and get final product, the inventive method is simple, cultivate effective, its biochemical bacteria purposes is wide, is worth greatly, and wide development application prospect and huge economic benefit are arranged.

Four, embodiment

Below in conjunction with practical situation the specific embodiment of the present invention is elaborated.

The present invention at first extracts rumen fluid, rumen fluid is meant the content or the gastric juice of ruminant domestic animal cud, method is sucking-off in the domestic animal cud that directly obtains from the healthy ruminant domestic animal stomach of slaughtering or ruminate from health with conduit, after adding water expansion bacterium liquid, add substratum again and carry out the former bacterium expansion bacterium cultivation of classification (the useful active biochemical bacteria that rumen fluid contains is called former bacterium), the biochemical expansion of elder generation's one-level is cultivated by bacterium, carry out secondary biochemical and bacterial classification subculture again, the biochemical former bacterium enlarged culturing of said one-level, be that to put into temperature in the seed cylinder be 45 ℃, it is standby that volume is that former bacterium water that the water of 6 times of amounts of rumen fluid is made 7 times of amounts of the former bacterium of rumen fluid mixes liquid, again will be by the straw powder 2% of mixing liquid weight in former bacterium water, fine fodder Semen Maydis powder or high-quality hay 0.5%, salt 0.1% and bicarbonate of ammonia 0.6%-0.8% mixing and stirring are made substratum, at pH value is 7.2 ± 0.2, when temperature is 42 ℃ ± 2 ℃, Manufactured former bacterium water is mixed liquid to be seeded in the substratum, seal with plastic cloth immediately, add a cover, cause anaerobic environment, seal after 2-3 days, observe filter paper disintegration situation, as decomposing fiber, the index of xylogen, disintegration gets final product as filter paper; And then carry out secondary biochemical and bacterial classification subculture;

Said secondary biochemical and bacterial classification subculture, be to enlarge training and grow water that good bacterium liquid adds 3 times of amounts of its volume and make secondary and expand bacterium liquid (be bacterium liquid behind the one-level biochemistry add water enlarge 4 times and become secondarys to expand the bacterium liquid) one-level is biochemical, inoculating at PH is 7.2 ± 0.2, when temperature is 42 ℃ ± 2 ℃ in the substratum, substratum is by expand bacterium liquid weight in secondary: straw powder 2%, fine fodder Semen Maydis powder or high-quality hay 0.5%, salt 0.1% and bicarbonate of ammonia 0.6-0.8% mixing and stirring are made, after the inoculation, seal with plastic cloth immediately, add a cover, cause anaerobic environment, seal after 2-3 days, the filter paper disintegration, the fiber rate of decomposition is 1.5-3%, and its protein increases by 50% approximately, promptly can be required active biochemical bacteria (liquid).The general sustainable 2-3 of bacterial classification subculture month.The fiber rate of decomposition of high quality seed (bacterium) liquid is generally 15%-30%, and true protein increases by 50% approximately.If do not reach this two indexs, and the biochemical ability drop of seed (bacterium) liquid, filter paper can not disintegration, the then production of hybrid seeds again in 2-3 days.If necessary, also can carry out multistage expansion bacterium such as three grades, level Four, same, the secondary of method, one by one stating more.

Said ruminant domestic animal is generally ox or sheep; In order to guarantee the sufficient amount of former bacterium, from the domestic animal cud of ruminating, getting the preceding quantity that as far as possible increases bacterial classification in the cud of rumen fluid (gastric juice in the stomach), method is, before extracting rumen fluid 3-5 days, give an amount of fine fodder Semen Maydis powder of ruminant domestic animal (ox, sheep) supplementary feeding or high-quality legume forage etc.Said straw powder is that corn stalk or wheat straw or peanut seedling or sweet potato vine are pulverized the powder of making.Fine fodder also can be wheat bran, concentrated feed commonly used, soya-bean cake, peanut cake etc.

All on sale on the above-mentioned said various raw materials markets.Huge meaning of the present invention is:

1, makes biochemical feed be used to feed pig, chicken, duck and fish etc. with high-efficiency activated biochemical bacteria, can substitute the fine fodder of 50%-80%, through experiment, feed pigs with the stalk biochemistry feed that contains 60%, day weight gain 550 grams, every pig can be saved the fine fodder double centner when delivering for sale, feeds duck 25 days, under the suitable situation of average every weightening finish, can save about 0.6 yuan of feeding cost.Fed 2-3 time in general day, every day, scale of feeding was no more than 80% of fine fodder.Stalk can improve greatly through nutritive ingredient after the biochemical treatment, quality deliquescing, glutinous, and have sour fragrance, domestic animal happiness food.Stalk behind the biochemistry has the robust fibre of 15%-20% to be decomposed, and reaches as high as 35%; True protein increases more than 50%, and contains 18 seed amino acids; Crude fat increases more than 60%, and voltaile fatty acid also significantly increases.Feed ox with biochemical feed, can substitute the fine fodder of 50%-90%.

2, the high-efficiency activated biochemical bacteria of the present invention's cultivation is used to produce fertilizer and not only contains nitrogen, phosphorus, potassium, trace element, and also has multivitamin, amino acid, saccharide compound.Plurality of enzymes such as matter compounds, Mierocrystalline cellulose, nucleic acid and SOD, effective chessom, culture fertility, preserve moisture and fertility, improve soil CEC value, reduce the EC value, improve ground temperature 2-4 degree, well developed root system, it is healthy and strong to grow, and percentage of fertile fruit improves 15-25%, ripen in advance 8-12 days, keep soil suitable substance P H value, the effect in improvement saltings is arranged; Reduce disease and pest and take place, reduce chemical pesticide consumption and number of times, improve crop quality, reduce the agricultural-food nitrate content greatly, pol improves 2-2.5 degree, storage endurance; Farm crop are improved output more than 25%, reduce cost, and the input-output ratio height, technology is grasped easily;

3, the high-efficiency activated biochemical bacteria of the present invention can be used for biochemical Chinese medicine, makes disinfectant use in agriculture, agrochemical.Medicament in the microbial biochemical, by materials such as the organic acid of metabolism generation, pure and mild enzymes, ore deposit element in the dissolving Chinese medicine, sterilization components such as the vegeto-alkali in decomposition, the conversion Chinese medicine, flavonoid, the rare class of terpene, improve drug effect more than 30 times, add tens kinds of high rhzomorphs that metabolism produces, can improve the effect of prevention and elimination of disease and pests greatly.Traditional Chinese medicine bactericide, sterilant can also the fiting chemical agricultural chemicals use, and not only can reduce half pesticide dosage, can also increase drug effect, and spray back 5-7 days can degrading pesticide, significantly reduce the residual quantity of agricultural chemicals in Pesticidal products.

4, cost is low, high efficiency.

The active biochemical bacteria of being made by the present invention is used to produce biochemical feed, and its biochemical feed is compared with the urea ammoniated feed, and cost only is 70% of a urea ammoniated feed.Ammoniated forage generally will add about 4% urea in stalk, promptly per 1000 kilograms of stalks need be with 40 kilograms urea; And biochemical feed only need add 0.5-1.0 kilogram active biochemical bacteria, has both reduced cost, alleviated livestock industry and agriculture contradiction of striving chemical fertilizer again, and meat is greatly improved also.

5, crop stalk is fully used, and has overcome the pollution that the burning because of straw causes air ambient.

In a word, of the present invention succeeding in developing, the application of having opened up little living mushroom, its popularization is come, and economic and social benefit is huge, makes to benefit the whole society.

Claims

1, a kind of cultivation preparation method of active biochemical bacteria, it is characterized in that, at first extract rumen fluid, carry out the former bacterium of classification again and expand the bacterium cultivation, method is sucking-off in the domestic animal cud that directly obtains from the healthy ruminant domestic animal stomach of slaughtering or ruminate from health with conduit, carry out the biochemical former bacterium enlarged culturing of one-level, promptly putting into temperature in the seed cylinder is 45 ℃, volume is the water of 6 times of amounts of rumen fluid, the mixed liquid of former bacterium water of making 7 times of amounts of the former bacterium of rumen fluid is standby, again will be by the straw powder 2% of mixing liquid weight in former bacterium water, fine fodder Semen Maydis powder or high-quality hay 0.5%, salt 0.1% and bicarbonate of ammonia 0.6%-0.8% mixing and stirring are made substratum, at pH value is 7.2 ± 0.2, when temperature is 42 ℃ ± 2 ℃, Manufactured former bacterium water is mixed liquid to be seeded in the substratum, seal with plastic cloth immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach the filter paper disintegration, and then carry out secondary biochemical and bacterial classification subculture, said secondary biochemical and bacterial classification subculture, be to enlarge training and grow water that good bacterium liquid adds 3 times of amounts of its volume and make secondary and expand bacterium liquid one-level is biochemical, inoculating at PH is 7.2 ± 0.2, when temperature is 42 ℃ ± 2 ℃ in the substratum, substratum is by expand bacterium liquid weight in secondary; Straw powder 2%, fine fodder Semen Maydis powder or high-quality hay 0.5%, salt 0.1% and bicarbonate of ammonia 0.6-0.8% mixing and stirring are made, after the inoculation, seal with plastic cloth immediately, add a cover, cause anaerobic environment, seal after 2-3 days, reach the filter paper disintegration, the fiber rate of decomposition is that 1.5-3%, protein increase by 50% approximately, is required active biochemical bacteria.

2, the cultivation preparation method of active biochemical bacteria according to claim 1 is characterized in that, said ruminant domestic animal is ox or sheep.

3, the cultivation preparation method of active biochemical bacteria according to claim 1 is characterized in that, before said extraction ruminant domestic animal rumen fluid 3-5 days, gives an amount of fine fodder or the high-quality legume forage of ruminant domestic animal supplementary feeding.

4, the cultivation preparation method of active biochemical bacteria according to claim 1 is characterized in that, the straw powder is that corn stalk or wheat straw or peanut seedling or sweet potato vine are pulverized the powder of making.