CN105250406A - Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof - Google Patents
Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof Download PDFInfo
- Publication number
- CN105250406A CN105250406A CN201510764165.0A CN201510764165A CN105250406A CN 105250406 A CN105250406 A CN 105250406A CN 201510764165 A CN201510764165 A CN 201510764165A CN 105250406 A CN105250406 A CN 105250406A
- Authority
- CN
- China
- Prior art keywords
- radix
- liquid
- herbal medicine
- chinese herbal
- chinese
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and a preparation method thereof. The Chinese herbal medicine fermentation extract liquid is formed by adding bacillus liquid into extract liquid of traditional Chinese medicine in a sealed anaerobic fermentation mode. The Chinese herbal medicine fermentation extract liquid is prepared from, by weight, 2-5 parts of rheum officinale, 2-4 parts of scutellaria baicalensis, 3-5 parts of honeysuckle, 1-2 parts of isatis roots and 1-2 parts of astragalus membranaceus. The Chinese herbal medicine fermentation extract liquid belongs to natural medicine, is free of toxic or side effect and free of drug residue and can achieve the function of preventing diseases as well as improving the immunity of aquatic livestock.
Description
Technical field
The invention belongs to Aquatic product technical field of Chinese medicine, be specifically related to a kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease and preparation method thereof.
Background technology
Although freshwater fish viral disease is uncommon in cultivation, harm is very serious, and especially in annual season in spring and autumn, be the multiple season of fishes virus disease, disease symptom is complicated and changeable, and infectiousness is strong, and mortality rate is high, there is no active drug treatment at present.The viral disease of fish mainly contains infectious hematopoietic necrosis, Pancreatic Necrosis disease, eel Mad swim disease, eel hemorrhagic dehisce disease, mandarin fish infectiousness Liver and kidney downright bad disease, hemorrhagic disease of grass carp, epithelioma papulosum cyprinorum etc.
It is less that fresh-water fishes viral disease Drug therapy is applied, and without efficient medicine, ordinary circumstance adopts disinfectant and antiviral chemicals to prevent and treat, but antiviral drugs just may produce infringement to host cell before inactivation of viruses, and a large amount of chemicals that uses not only can reduce flesh of fish matter quality, and meeting residual toxicity, and then pollute water body environment, reduce the resistance against diseases of Fish self.So the protective agents of development nonhazardous is an important directions of antiviral fish medicine development.Chinese herbal medicine has immunologic active material, can enhancing human body immunity power and regulate body constitution, also can play the effect of deactivation or suppression to virus, and few side effects, toxicity are low, the advantage such as do not develop immunity to drugs because its natural sex has.So it has certain superiority to the control of fishes virus disease.Bacillus cereus is a class aerobic bacteria, can form endospore when environment is bad.Bacillus cereus can produce active very high protease, amylase and lipase, to degrade in addition the enzyme of complex compound in feedstuff simultaneously, not only can decompose rapidly the Organic substances such as residual bait, Excreta and the animal residue in water body, the Eutrophic Extent of obvious reduction breeding water body, purify water, reduce disease to occur, effectively can also improve efficiency of feed utilization, promote aquatic animal to grow.
The culture rarity of Chinese veterinarian Yao Shi China preciousness, with a long history by Chinese herbal treatment Animal diseases, there is again abundant Chinese herbal medicine resource in China simultaneously, utilize Chinese medicine as the key agents of animal diseases control, both solve the drug resistance problems of pathogenic bacterium, turn eliminate the pollution problem that chemical drugs brings to environment.From Chinese herbal medicine, develop novel feed additive and drugs used in aquiculture, the culture fishery of China will be made to develop into pollution-free industry, also enter international market smoothly pave the way for the aquatic products of China can draw.Microbial ecological agent is mainly used in improving water quality, the body health strengthening fish, the commodity nature improving fish and kind performance in aquaculture.Along with the needs of aquatic ecosystem healthy aquaculture, people increase the research dynamics of green, environmental protection medicine and additive, just because of probiotic bacteria and Chinese herbal and crude drugs preparations is nontoxic, noresidue, free of contamination characteristic, the use in conjunction on aquaculture is also increasingly extensive.
Summary of the invention
The object of the present invention is to provide a kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease and preparation method thereof, this Chinese herbal medicine fermented extracted liquid belongs to natural drug, have no side effect, more drug residue free, not only can play the effect of disease preventing and treating, and improve the immunity of aquatic animal.
In order to achieve the above object, the present invention is by the following technical solutions:
The invention provides a kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2 ~ 5, Radix Scutellariae 2 ~ 4, Flos Lonicerae 3 ~ 5, Radix Isatidis 1 ~ 2 and the Radix Astragali 1 ~ 2.
According to the Chinese herbal medicine fermented extracted liquid of above-mentioned control fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2 ~ 3, Radix Scutellariae 3 ~ 4, Flos Lonicerae 3 ~ 4, Radix Isatidis 1 ~ 1.5 and the Radix Astragali 1.5 ~ 2.
According to the Chinese herbal medicine fermented extracted liquid of above-mentioned control fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3, Radix Scutellariae 4, Flos Lonicerae 5, Radix Isatidis 2 and the Radix Astragali 1.
The preparation method of the Chinese herbal medicine fermented extracted liquid of described control fresh-water fishes viral disease, concrete steps are as follows:
(1) Chinese herb rhubarb, Radix Scutellariae, Flos Lonicerae, Radix Isatidis and the Radix Astragali being dried, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) get according to above-mentioned formula the water boiling and extraction that step (1) Chinese medicinal powder adds 8 ~ 10 times amount Chinese medicinal powder amounts, decoct and filter to get filtrate for 1 ~ 2 hour; Medicinal residues add the water boiling and extraction of 8 ~ 10 times of medicinal residues amounts, and decoct and filter to get filtrate for 1 ~ 2 hour, merging filtrate is for subsequent use;
(3) getting bacillus cereus bacterium liquid by weight adds in step (2) gained filtrate, weight ratio 1:(40 ~ 60 of bacillus cereus bacterium liquid and herb liquid), closing anaerobic ferments, and subpackage, must prevent and treat the Chinese herbal medicine fermented extracted liquid of fresh-water fishes viral disease.
The preparation method of described bacillus cereus bacterium liquid is as follows: get Bacillus and carry out slant strains cultivation, by suspension anaerobism amplification culture in the culture medium of correspondence that slant culture produces, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained.
Step (1) bake out temperature is 60 ~ 80 DEG C.
In step (3), fermentation temperature is 30 DEG C ~ 37 DEG C, and fermentation time is 24 ~ 48h.
The culture medium of bacillus cereus is nutrient agar.
in the present invention, each raw material effect is summarized as follows:
Radix Et Rhizoma Rhei: have another name called bright and beautiful stricture of vagina, Huang Liang, another name general, raw army, R.hoofshaped etc., herbaceos perennial.Be used as medicine with root, rhizome, antibacterial action is strong, has a broad antifungal spectrum, under having convergence, rushing down, heat clearing away, removing toxic substances, removing mass, blood stasis removing, increase platelet, promotes the effects such as blood coagulation.For gill rot, gill rot, hemorrhagic disease and white head and mouth fish disease etc.Radix Scutellariae: herbaceos perennial.Be used as medicine with root, have antibacterial, antiviral, calmness, diuresis, guarantor's gallbladder, protect the liver, detoxify, antiinflammatory and make the effects such as blood glucose rising.Be mainly used in preventing and treating gill rot, furuncle, stigmatosis, bovine pasteurellosis, enteritis, caudal peduncle disease etc.
Flos Lonicerae: have another name called Radix Ophiopogonis.Flos Lonicerae can dispelling wind-heat, also kind removing summer-heat blood poison, for various febrile disease, as fever of the body, dermexanthesis, sends out the diseases such as speckle, pyretic toxicity carbuncle sore, laryngopharynx swelling and pain, equal Be very effective.The diseases such as fish beancurd sheet, enteritis can be prevented and treated.
Radix Isatidis: have another name called indigo-blue, Isatis indigotica Fort (Indigofera tinctoria L, Baphicanthus cusia (nees) Brem. Polygonum tinctorium Ait) root, Radix Clerodenri Cyrtophylli.It is cold in nature, and taste is micro-sweet rear bitterness first, has effect of heat-clearing and toxic substances removing, preventing cold, sore-throat relieving.Fish rotten cheek, enteritis, hemorrhage etc. can be prevented and treated.
The Radix Astragali: have another name called continuous stilbene.Main product in the Inner Mongol, Shanxi, Gansu, the ground such as Heilungkiang.The sweet temperature of the Radix Astragali, returns lung meridian, has tonifying Qi and lifting yang, a function that benefit defends consolidating superficial resistance.The Radix Astragali has enhancing human body immunity function, protects the liver, diuresis, anti-stress and antibacterial action more widely.
Bacillus cereus: bacillus cereus belongs to gram positive bacteria, aerobic, can produce spore, be the heterotrophic bacteria that a class has highly active Digest enzyme, high temperature resistant, anti-stress.It can decompose carbon system, nitrogen system, phosphorus system, sulfur system pollutant consumingly, decomposing protein and complicated polysaccharide, water soluble organic substance is decomposed and also has important effect, can compete with the harmful algae in breeding environment and aquatic pathogenic bacteria simultaneously, form dominant population, suppress harmful algae and aquatic pathogenic bacteria.So the application of bacillus cereus in aquaculture is more and more extensive.
compared with prior art, the beneficial effect that obtains of the present invention:
1. Chinese herbal medicine fermented extracted liquid of the present invention belongs to natural drug, has no side effect, more drug residue free, not only can play the effect of disease preventing and treating, and can improve the immunity of aquatic animal.
2. the present invention can be competed with the harmful algae in breeding environment and aquatic pathogenic bacteria by adding of bacillus cereus, form dominant population, suppress harmful algae and aquatic pathogenic bacteria, be conducive to aquaculture, improve breeding environment, decompose water soluble organic substance also has important effect simultaneously.
3. what the present invention adopted Chinese medicine and probiotic bacteria is combined with significant economic benefit and social benefit.
usage and dosage: method of splashing.Prophylactic, this product every bottle 1000ml scope of splashing is 1000ml/10m
2, be used in conjunction 2 ~ 3 days.Therapeutic dose doubles, and is used in conjunction 5 ~ 8 days.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described, but do not limit content of the present invention.
embodiment 1
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2 ~ 5, Radix Scutellariae 2 ~ 4, Flos Lonicerae 3 ~ 5, Radix Isatidis 1 ~ 2 and the Radix Astragali 1 ~ 2.
The preparation method of the Chinese herbal medicine fermented extracted liquid of described control fresh-water fishes viral disease, concrete steps are as follows:
(1) Chinese herb rhubarb, Radix Scutellariae, Flos Lonicerae, Radix Isatidis and the Radix Astragali being dried at temperature is 60 ~ 80 DEG C, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) get according to above-mentioned formula the water boiling and extraction that step (1) Chinese medicinal powder adds 8 ~ 10 times amount Chinese medicinal powder amounts, decoct and filter to get filtrate for 1 ~ 2 hour; Medicinal residues add the water boiling and extraction of 8 ~ 10 times of medicinal residues amounts, and decoct and filter to get filtrate for 1 ~ 2 hour, merging filtrate is for subsequent use;
(3) getting bacillus cereus bacterium liquid by weight adds in step (2) gained filtrate, weight ratio 1:(40 ~ 60 of bacillus cereus bacterium liquid and herb liquid), closing anaerobic ferments, fermentation temperature is 30 DEG C ~ 37 DEG C, fermentation time is 24 ~ 48h, subpackage, must prevent and treat the Chinese herbal medicine fermented extracted liquid of fresh-water fishes viral disease.
The preparation method of described bacillus cereus bacterium liquid is as follows: get Bacillus and carry out slant strains cultivation, by suspension anaerobism amplification culture in the nutrient agar of correspondence that slant culture produces, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained.
embodiment 2
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3, Radix Scutellariae 4, Flos Lonicerae 5, Radix Isatidis 2 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 3
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2, Radix Scutellariae 3, Flos Lonicerae 5, Radix Isatidis 1 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 4
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 5, Radix Scutellariae 2, Flos Lonicerae 3, Radix Isatidis 2 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 5
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3, Radix Scutellariae 3, Flos Lonicerae 4, Radix Isatidis 1 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 6
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2, Radix Scutellariae 4, Flos Lonicerae 3, Radix Isatidis 2 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 7
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 4, Radix Scutellariae 4, Flos Lonicerae 3, Radix Isatidis 1 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 8
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2, Radix Scutellariae 2, Flos Lonicerae 3, Radix Isatidis 2 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 9
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3, Radix Scutellariae 4, Flos Lonicerae 5, Radix Isatidis 2 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 10
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2, Radix Scutellariae 2, Flos Lonicerae 5, Radix Isatidis 1 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 11
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 5, Radix Scutellariae 3, Flos Lonicerae 4, Radix Isatidis 2 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 12
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 4, Radix Scutellariae 2, Flos Lonicerae 3.5, Radix Isatidis 2 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 13
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 5, Radix Scutellariae 2, Flos Lonicerae 2.5, Radix Isatidis 1.5 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 14
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3.5, Radix Scutellariae 3, Flos Lonicerae 3.5, Radix Isatidis 1.5 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 15
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2.5, Radix Scutellariae 3.5, Flos Lonicerae 4.5, Radix Isatidis 1 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 16
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3.5, Radix Scutellariae 4, Flos Lonicerae 3.5, Radix Isatidis 1.5 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 17
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 4.5, Radix Scutellariae 2.5, Flos Lonicerae 4, Radix Isatidis 1.5 and the Radix Astragali 2.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 18
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3.5, Radix Scutellariae 2.5, Flos Lonicerae 3.5, Radix Isatidis 1.5 and the Radix Astragali 1.5.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 19
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3.5, Radix Scutellariae 4, Flos Lonicerae 3.5, Radix Isatidis 2 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
embodiment 20
A kind of Chinese herbal medicine fermented extracted liquid preventing and treating fresh-water fishes viral disease, be added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2.5, Radix Scutellariae 2.5, Flos Lonicerae 5, Radix Isatidis 1 and the Radix Astragali 1.
The preparation method of the present embodiment is identical with embodiment 1.
With specific experiment, effect of the present invention is described below:
Test example
Experimental animal: random selecting 400 tail outward appearance is normal, and body constitution is strong, the Ctenopharyngodon idellus of the even about 50g of size.Be divided into and be placed in cultivation in indoor 4 ponds, 100 tail Ctenopharyngodon idellus in each pond.
Test group: test 1 ~ 3 group, the Chinese herbal medicine fermented extracted liquid of preparation in embodiment of splashing respectively 2 ~ 4, matched group does not give any therapeutic regimen, the bait that feed is identical.
Test method: 4 groups, matched group does not add any medicine, a feed basal diet.Other three groups, press 1000ml/10m week about
2dosage to splash a Chinese herbal medicine fermented extracted liquid.Test period is 8 weeks.Period is sooner or later each throws a bait, and it is 15 ~ 20 DEG C that water temperature controls, air compressor according to circumstances regular oxygenation, observes every day and records the health condition of fish; Duration of test does not change water, the residual bait of periodic cleaning and feces.
Curative effect judging standard:
Morbidity: in test, the disease (body surface has obvious pathological changes) of viral disease appears in Ctenopharyngodon idellus;
Dead: the morbidity of off-test Ctenopharyngodon idellus is dead;
Survival: duration of test occurs that symptom does not have Ctenopharyngodon idellus that is dead and no morbidity.
Conclusion: statistical analysis, in test group 1 ~ 3 group, the sickness rate of Ctenopharyngodon idellus is all remarkable in matched group (P < 0.01), and survival rate is higher than 90%.The morbidity number of test group 1 and death toll are starkly lower than test group 2 and 3, and survival number, apparently higher than test group 2 and 3, illustrates that preferred example of the present invention is embodiment 2.As can be seen here, fresh-water fishes viral disease of the present invention has good preventive and therapeutic effect.
Claims (8)
1. prevent and treat the Chinese herbal medicine fermented extracted liquid of fresh-water fishes viral disease for one kind, it is characterized in that being added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2 ~ 5, Radix Scutellariae 2 ~ 4, Flos Lonicerae 3 ~ 5, Radix Isatidis 1 ~ 2 and the Radix Astragali 1 ~ 2.
2. the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 1, it is characterized in that being added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 2 ~ 3, Radix Scutellariae 3 ~ 4, Flos Lonicerae 3 ~ 4, Radix Isatidis 1 ~ 1.5 and the Radix Astragali 1.5 ~ 2.
3. the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 1, it is characterized in that being added by the extracting solution of Chinese medicine that bacillus cereus bacterium is liquid-tight to be closed anaerobic fermentation and form, wherein said Chinese medicine is made up of the component of following weight portion: Radix Et Rhizoma Rhei 3, Radix Scutellariae 4, Flos Lonicerae 5, Radix Isatidis 2 and the Radix Astragali 1.
4. a preparation method for the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease as claimed in claim 1, is characterized in that concrete steps are as follows:
(1) Chinese herb rhubarb, Radix Scutellariae, Flos Lonicerae, Radix Isatidis and the Radix Astragali being dried, pulverizing is that 40 ~ 80 order powder are for subsequent use;
(2) get according to above-mentioned formula the water boiling and extraction that step (1) Chinese medicinal powder adds 8 ~ 10 times amount Chinese medicinal powder amounts, decoct and filter to get filtrate for 1 ~ 2 hour; Medicinal residues add the water boiling and extraction of 8 ~ 10 times of medicinal residues amounts, and decoct and filter to get filtrate for 1 ~ 2 hour, merging filtrate is for subsequent use;
(3) getting bacillus cereus bacterium liquid by weight adds in step (2) gained filtrate, weight ratio 1:(40 ~ 60 of bacillus cereus bacterium liquid and herb liquid), closing anaerobic ferments, and subpackage, must prevent and treat the Chinese herbal medicine fermented extracted liquid of fresh-water fishes viral disease.
5. the preparation method of the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 4, is characterized in that the preparation method of bacillus cereus bacterium liquid is as follows:
Get Bacillus and carry out slant strains cultivation, by suspension anaerobism amplification culture in the culture medium of correspondence that slant culture produces, to exponential phase clump count higher than 1.0 × 10
10during CFU/mL only, corresponding bacterium liquid is obtained.
6. the preparation method of the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 4, is characterized in that: step (1) bake out temperature is 60 ~ 80 DEG C.
7. the preparation method of the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 4, is characterized in that: in step (3), fermentation temperature is 30 DEG C ~ 37 DEG C, and fermentation time is 24 ~ 48h.
8. the preparation method of the Chinese herbal medicine fermented extracted liquid of control fresh-water fishes viral disease according to claim 5, is characterized in that: the culture medium of bacillus cereus is nutrient agar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510764165.0A CN105250406A (en) | 2015-11-11 | 2015-11-11 | Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510764165.0A CN105250406A (en) | 2015-11-11 | 2015-11-11 | Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105250406A true CN105250406A (en) | 2016-01-20 |
Family
ID=55090541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510764165.0A Pending CN105250406A (en) | 2015-11-11 | 2015-11-11 | Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105250406A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106728967A (en) * | 2017-01-10 | 2017-05-31 | 杨涛 | It is a kind of to treat medicine of hypertension and preparation method thereof |
| CN109675001A (en) * | 2019-01-25 | 2019-04-26 | 沧州北通达海洋工程机械科技有限公司 | The Chinese medicine composition for preventing and treating cage culture fresh water fish disease |
| CN112156175A (en) * | 2020-11-09 | 2021-01-01 | 柳州市绿威生物科技有限公司 | Composition for degrading drug residues in live fish and preparation method thereof |
| CN112853002A (en) * | 2021-02-23 | 2021-05-28 | 广州力必拓生物科技有限公司 | Method for preventing and controlling iridovirus disease of Micropterus salmoides |
| CN112899399A (en) * | 2021-02-03 | 2021-06-04 | 广州力必拓生物科技有限公司 | Accurate prevention and control overall solution technical scheme for infectious spleen and kidney necrosis virus diseases of mandarin fish |
| CN112921118A (en) * | 2021-02-03 | 2021-06-08 | 广州力必拓生物科技有限公司 | Accurate prevention and control method for infectious spleen and kidney necrosis virus disease of micropterus salmoides |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433795A (en) * | 2002-01-23 | 2003-08-06 | 贵州海盾生物科技开发有限责任公司 | Traditional Chinese granule medicine for preventing and caring prawn viral and bacterial disease |
| WO2010095858A2 (en) * | 2009-02-17 | 2010-08-26 | 한국생명공학연구원 | Pharmaceutical composition containing rhei rhizoma extract, fractions thereof, or stilbene-based compounds for preventing or treating diseases mediated by intercellular adhesion molecule-1 |
| CN102068492A (en) * | 2011-01-05 | 2011-05-25 | 湖南时代阳光药业股份有限公司 | Anti-virus Chinese herbal medicine composition, traditional Chinese medicine composition and preparation method thereof |
| CN102885901A (en) * | 2011-07-20 | 2013-01-23 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating black gill disease of fishes |
-
2015
- 2015-11-11 CN CN201510764165.0A patent/CN105250406A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433795A (en) * | 2002-01-23 | 2003-08-06 | 贵州海盾生物科技开发有限责任公司 | Traditional Chinese granule medicine for preventing and caring prawn viral and bacterial disease |
| WO2010095858A2 (en) * | 2009-02-17 | 2010-08-26 | 한국생명공학연구원 | Pharmaceutical composition containing rhei rhizoma extract, fractions thereof, or stilbene-based compounds for preventing or treating diseases mediated by intercellular adhesion molecule-1 |
| CN102068492A (en) * | 2011-01-05 | 2011-05-25 | 湖南时代阳光药业股份有限公司 | Anti-virus Chinese herbal medicine composition, traditional Chinese medicine composition and preparation method thereof |
| CN102885901A (en) * | 2011-07-20 | 2013-01-23 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating black gill disease of fishes |
Non-Patent Citations (2)
| Title |
|---|
| 李清: "《水生动物疾病与安全用药手册》", 30 April 2014, 海洋出版社 * |
| 高春生等: "《渔场多发疾病防控手册》", 31 March 2012, 河南科学技术出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106728967A (en) * | 2017-01-10 | 2017-05-31 | 杨涛 | It is a kind of to treat medicine of hypertension and preparation method thereof |
| CN109675001A (en) * | 2019-01-25 | 2019-04-26 | 沧州北通达海洋工程机械科技有限公司 | The Chinese medicine composition for preventing and treating cage culture fresh water fish disease |
| CN112156175A (en) * | 2020-11-09 | 2021-01-01 | 柳州市绿威生物科技有限公司 | Composition for degrading drug residues in live fish and preparation method thereof |
| CN112899399A (en) * | 2021-02-03 | 2021-06-04 | 广州力必拓生物科技有限公司 | Accurate prevention and control overall solution technical scheme for infectious spleen and kidney necrosis virus diseases of mandarin fish |
| CN112921118A (en) * | 2021-02-03 | 2021-06-08 | 广州力必拓生物科技有限公司 | Accurate prevention and control method for infectious spleen and kidney necrosis virus disease of micropterus salmoides |
| CN112853002A (en) * | 2021-02-23 | 2021-05-28 | 广州力必拓生物科技有限公司 | Method for preventing and controlling iridovirus disease of Micropterus salmoides |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105250406A (en) | Chinese herbal medicine fermentation extract liquid for preventing and treating viral diseases of freshwater fish and preparation method thereof | |
| CN105174493B (en) | A kind of preparation method and applications of fermented type Chinese herbal medicine improver of water quality used for aquiculture | |
| CN102550875B (en) | Compound pig feed additive with effect of improving pig immunity | |
| CN102366023A (en) | Method for preparing cow feed additive containing traditional Chinese medicine active probiotics | |
| CN114601076B (en) | Compound feed for promoting mandarin fish ingestion and preventing and treating mandarin fish virus diseases and preparation method thereof | |
| CN107432263A (en) | The foster method of full ecological fish duck set | |
| CN105767543A (en) | Stomach-strengthening and digestion-promoting traditional Chinese medicine micro-ecological preparation for piglet and preparation method thereof | |
| CN105194041A (en) | Chinese herbal medicinal fermented extract liquid for preventing and treating bacterial diseases of freshwater fish and preparation method of Chinese herbal medicinal fermented extract liquid | |
| CN106343255A (en) | Feed capable of promoting toxin expelling of freshwater shrimps and preparation method thereof | |
| CN105232850A (en) | Chinese herbal medicine fermentative extracting solution for preventing and controlling parasitic diseases of freshwater fish and preparation method thereof | |
| CN104887843B (en) | Traditional Chinese medicine composition for preventing and treating fish gill rot disease and preparation method thereof | |
| CN107233428A (en) | A kind of fishes and shrimps enteritis pesticide control and preparation method thereof | |
| CN102764308A (en) | Drug for preventing bacterial diseases of fish and preparation method thereof | |
| CN109497310A (en) | One boar combines Se-enriched feedstuff with Chinese herbal medicine | |
| CN107714799A (en) | A kind of Chinese medicine composition and preparation method for being used to prevent and treat diarrhea of pigs | |
| CN101647519A (en) | Traditional Chinese medicine feed additive for treating infectious fowl pox disease | |
| CN106039138A (en) | Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture | |
| CN102640861B (en) | Fish feed containing traditional Chinese medicine component for preventing fish enteritis | |
| CN104147467B (en) | Traditional Chinese medicine for treating endotoxemia of poultry and livestock and preparation process thereof | |
| CN112933194A (en) | Traditional Chinese medicine composition and traditional Chinese medicine preparation for preventing and treating fish ichthyophthirius multifiliis | |
| CN106359473A (en) | Fermented bacteria liquid capable of preventing and controlling pawpaw mosaic diseases and preparation method thereof | |
| CN110050903A (en) | A kind of cultivation Chinese herbal medicine ferment and preparation method thereof | |
| CN105168310A (en) | Traditional Chinese medicine composition used for bacterial diseases of turtles | |
| CN101879238A (en) | Medicinal composition for preventing and controlling chicken colibacillosis and preparation method thereof | |
| CN111419954A (en) | Antiviral traditional Chinese medicine composition for shrimps and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160120 |