CN104887843B - Traditional Chinese medicine composition for preventing and treating fish gill rot disease and preparation method thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating fish gill rot disease and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for preventing and treating gill rot diseases of fishes and a preparation method thereof. The traditional Chinese medicine composition contains active ingredients, wherein the active ingredients are prepared from rhubarb, houttuynia cordata, verbena, sophora flavescens and gallnut. The traditional Chinese medicine composition has the effects of clearing away heat and toxic materials, resisting bacteria and killing insects, astringing to stop bleeding and promoting tissue regeneration, can be effectively used for preventing and treating gill rot diseases of fishes caused by various factors, and has the advantages of wide raw material source, safety, environmental protection, simple preparation process, low production cost and the like.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition for preventing and treating fish gill rot diseases and a preparation method thereof, and belongs to the field of veterinary medicines.
Background
At present, the continuous deep development of the large-scale aquaculture industry in China has the problems that fish diseases frequently outbreak, especially fish gill rot diseases cause huge loss to the aquaculture industry in China, and the fish gill rot diseases become important factors influencing and restricting the development of the aquaculture industry in China. The occurrence of gill rot, enteritis, red skin disease and hemorrhagic disease of grass carp is subjected to popular observation and recording for 6 years in Nanning city by the research institute of aquatic products in Guangxi, and the research and statistical results show that the average annual incidence rate of the gill rot disease is 62.5%, the economic loss caused by the disease is far more than the influence caused by other diseases, and the gill rot disease becomes an important reason for economic loss. The most direct damage of gill rot disease of fishes is that the production performance of the fishes is reduced, even a great amount of fishes die seriously, and meanwhile, the culture cost of farmers is increased.
At present, most of the medicines for preventing and treating fish gill rot diseases are antibiotics and chemically synthesized antibacterial medicines, such as enrofloxacin, doxycycline, florfenicol, sulfonamides, berberine hydrochloride, benzimidazoles and the like. Due to the wide application of antibacterial drugs, the drug-resistant strains are more and more, so that the treatment effect of the drugs is reduced, and the gill rot disease is more and more difficult to control. Meanwhile, the use of chemical drugs also affects the safety of animal food and is harmful to human health. Chinese patents CN103169747A, CN101961034A and CN103169780A are medicines for treating the fish diseases, and are all prepared by mixing traditional Chinese medicines and western medicines (ciprofloxacin, florfenicol, isoniazid and the like), wherein the western medicines have high cost and certain drug resistance and toxic and side effects.
The traditional Chinese herbal medicine is natural organic matter, and has the characteristics of safety, low toxicity, difficulty in generating drug resistance, low residue and the like. The complex chemical components of the Chinese herbal medicines are the material basis of the multi-component combined effect, the traditional Chinese herbal medicines are mostly compound compounds formed by matching a plurality of medicines, and are different from western medicines, the complex components in the traditional Chinese herbal medicines can generate the multi-component effect on the fish body, have a plurality of action targets and have the combined effect. The traditional Chinese herbal medicines can improve the production performance of fish, improve meat quality, enhance immune function, reduce stress, delay disease onset, relieve symptoms, promote recovery of pathological changes, prevent and treat complications or secondary infection, strengthen body resistance, eliminate pathogenic factors and take both principal and secondary aspects into consideration. In addition, the Chinese herbal medicines do not destroy normal flora in intestinal tracts and in water body environment, are beneficial to the growth of beneficial bacteria, can improve the feed conversion rate and reduce the culture cost while preventing and treating diseases. Chinese patents CN103432261A and CN102716247A are pure traditional Chinese medicine compositions for treating the fish diseases, but the former medicine compositions have more components and higher cost, and the latter medicine compositions are oral liquid, are inconvenient to use in actual production and are not suitable for large-scale cultivation medicine.
Disclosure of Invention
The invention aims to provide a safe and environment-friendly traditional Chinese medicine composition for effectively preventing and treating fish gill rot diseases, which can be effectively used for enhancing the immunity of aquaculture animals, improving the body resistance and preventing and treating the fish gill rot diseases.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition for preventing and treating the fish gill-rot disease.
The purpose of the invention is realized by the following technical scheme:
the invention provides a traditional Chinese medicine composition for preventing and treating fish gill rot diseases, which contains active ingredients, wherein the active ingredients are prepared from rheum officinale, houttuynia cordata, verbena officinalis, sophora flavescens and gallnut.
Wherein the raw materials comprise the following components in parts by weight: 1-50 parts of rheum officinale, 1-30 parts of houttuynia cordata, 1-30 parts of verbena, 1-30 parts of sophora flavescens and 1-20 parts of gallnut.
Further, the raw materials comprise the following components in parts by weight: 5-40 parts of rheum officinale, 5-25 parts of houttuynia cordata, 5-25 parts of verbena officinalis, 5-25 parts of sophora flavescens and 2-15 parts of gallnut.
Furthermore, the raw materials comprise the following components in parts by weight: 10-30 parts of rheum officinale, 10-20 parts of houttuynia cordata, 10-20 parts of verbena officinalis, 10-20 parts of sophora flavescens and 5-10 parts of gallnut.
The traditional Chinese medicine composition is a traditional Chinese medicine extract.
The traditional Chinese medicine composition can be directly added into feed to prevent and treat gill rot diseases of aquatic animals.
The invention also provides a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
weighing the raw materials according to the weight parts, crushing, adding water which is 5-15 times of the total weight of the medicines, decocting for 1-3 times, 1-3 hours each time, combining the decoctions, filtering, standing the filtrate for 6-12 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
The principle of the formula of the invention is as follows:
1) rhubarb: has strong inhibiting effect on most gram positive and negative bacteria, has antiviral effect, and has hemostatic and coagulation promoting effects;
2) houttuynia cordata: has the effect of inhibiting the growth of various microorganisms. Can regulate body defense factors of animals and improve immunity. Simultaneously has the functions of easing pain, stopping bleeding, inhibiting serous secretion, promoting tissue regeneration and the like;
3) verbena: clearing away heat and toxic materials, inhibiting bacteria, and stopping bleeding;
4) flavescent sophora root: clear heat and dry dampness, kill parasites and remove accumulation, induce diuresis. Against common pathogenic fungi;
5) gallnut: has effects in resisting gram-positive and gram-positive bacteria. Has good astringing effect on skin, mucosa, ulcer, etc. Has certain inhibitory effect on dermatophytes. Can accelerate blood coagulation and stop bleeding.
The invention has the advantages and positive effects that:
1) the traditional Chinese medicine composition can enhance the organism immunity of aquaculture animals and improve the disease resistance;
2) the traditional Chinese medicine composition is a traditional Chinese medicine extract, has obvious curative effect, quick response and small dosage, and can effectively prevent and treat gill rot diseases of fishes;
3) the traditional Chinese medicine composition is convenient to use and can be uniformly mixed;
4) the traditional Chinese medicine composition is prepared from natural traditional Chinese medicinal materials, is safe and environment-friendly, has small toxic and side effects, and does not generate drug resistance and drug residues.
The drug formula of the invention selects drugs according to the pharmacological action aiming at different causes and symptoms of gill rot of fishes, can comprehensively solve the gill rot disease of fishes, and can take the root cause and symptoms into consideration. The Chinese veterinary medicine is developed by adopting natural Chinese herbal medicine raw materials through processing measures such as extraction and the like, and is efficient, safe, green and environment-friendly. Compared with the prior art, the medicament has the advantages of pure Chinese medicinal formula, only five Chinese medicinal materials, wide raw material source, safety, environmental protection, simple preparation process and low production cost, and can prevent and treat gill rot diseases of fishes caused by various factors. The medicine can improve the drug effect and curative effect of the Chinese herbal medicine, solve the problems of slow effect and poor curative effect of the Chinese herbal medicine, and reduce the toxic and side effect of the medicine; in addition, the problems of drug residue, drug resistance and the like caused by the application of the existing health-care drugs can be avoided, the health and safety of aquaculture animals are promoted, and the safety of animal food is guaranteed.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
50 parts of rhubarb, 30 parts of heartleaf houttuynia herb, 30 parts of verbena, 1 part of lightyellow sophora root and 20 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 10 times of the total weight of the five medicines, decocting for 2 times, decocting for 2 hours each time, combining the decoctions, filtering, standing the filtrate for 6 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 2
10 parts of rhubarb, 20 parts of heartleaf houttuynia herb, 20 parts of verbena, 20 parts of lightyellow sophora root and 10 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 5 times of the total weight of the five medicines, decocting for 2 times, 3 hours each time, combining the decoctions, filtering, standing the filtrate for 9 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 3
50 parts of rhubarb, 30 parts of heartleaf houttuynia herb, 30 parts of verbena, 30 parts of lightyellow sophora root and 1 part of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 15 times of the total weight of the five medicines, decocting for 3 times and 2 hours each time, combining the decoctions, filtering, standing the filtrate for 6 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 4
50 parts of rhubarb, 1 part of heartleaf houttuynia herb, 30 parts of verbena, 30 parts of lightyellow sophora root and 1 part of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 10 times of the total weight of the five medicines, decocting for 2 times, 2 hours each time, combining the decoctions, filtering, standing the filtrate for 6 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 5
50 parts of rhubarb, 30 parts of heartleaf houttuynia herb, 1 part of verbena, 1 part of lightyellow sophora root and 20 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 10 times of the total weight of the five medicines, decocting for 2 times, 2 hours each time, combining the decoctions, filtering, standing the filtrate for 9 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 6
35 parts of rhubarb, 20 parts of heartleaf houttuynia herb, 20 parts of verbena, 10 parts of lightyellow sophora root and 13 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 5 times of the total weight of the five medicines, decocting for 1 time and 3 hours each time, combining decoctions, filtering, standing the filtrate for 12 hours, taking supernate, concentrating, and drying to be powder to obtain the traditional Chinese medicine composition.
Example 7
15 parts of rhubarb, 10 parts of heartleaf houttuynia herb, 10 parts of verbena, 10 parts of lightyellow sophora root and 13 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 15 times of the total weight of the five medicines, decocting for 3 times and 2 hours each time, combining the decoctions, filtering, standing the filtrate for 12 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 8
30 parts of rhubarb, 10 parts of heartleaf houttuynia herb, 20 parts of verbena, 20 parts of lightyellow sophora root and 10 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 10 times of the total weight of the five medicines, decocting for 2 times, 2 hours each time, combining the decoctions, filtering, standing the filtrate for 9 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 9
20 parts of rhubarb, 15 parts of heartleaf houttuynia herb, 15 parts of verbena, 15 parts of lightyellow sophora root and 5 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 10 times of the total weight of the five medicines, decocting for 2 times, 3 hours each time, combining the decoctions, filtering, standing the filtrate for 9 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 10
10 parts of rhubarb, 10 parts of heartleaf houttuynia herb, 15 parts of verbena, 15 parts of lightyellow sophora root and 5 parts of Chinese gall
Weighing the raw materials according to the weight, crushing, adding water which is 15 times of the total weight of the five medicines, decocting for 3 times and 2 hours each time, combining the decoctions, filtering, standing the filtrate for 6 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Example 11
30 parts of rhubarb, 10 parts of heartleaf houttuynia herb, 15 parts of verbena, 15 parts of lightyellow sophora root and 10 parts of Chinese gall.
Weighing the raw materials according to the weight, crushing, adding water which is 5 times of the total weight of the five medicines, decocting for 1 time and 3 hours each time, combining the decoctions, filtering, standing the filtrate for 12 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
Experimental example 1 observation of prevention and treatment effects of bacterial gill-rot disease of grass carp using the pharmaceutical composition of traditional Chinese medicine prepared in example 1 of the present invention
In the last 5 th month of 2013, the experiment was carried out in the clinical laboratory of Dabei agricultural fish farming. The grass carp of clinical trial was purchased from Huairou south China wholesale market in Beijing, with a specification of about 50 g of healthy grass carp, and 270 tails, and temporarily cultured in an aquarium in a laboratory for 5 days. Experiments are divided into 3 groups, the group 1 is the medicine group, the traditional Chinese medicine composition prepared in the embodiment 1 of the invention is fed after the gill rot disease is infected, the medicine in the embodiment 1 of the invention is added according to the weight of 100 mg/kg of fish body, and the continuous 5 days are carried out; the group 2 is an infection control group, and no medicine is fed after the gill rot disease is infected; group 3 was a healthy control group, and was not administered for infection. 30 of each group were provided with 3 replicates, as shown in Table 1. Using a cylindrical suspension of Cellophilus (10)4cfu/mL) on the gill filaments of the experimental fishes of the infection control group and the drug group, and the gill rot symptoms of the fishes are all appeared after 3 days. The test water is aerated tap water with pH 7.3, ammoniacal nitrogen 0.50 mg/L and nitrous acid nitrogen 0.02 mg/L, and is continuously aerated with water temperature (28 +/-1) DEG C.
The calculation method comprises the following steps: mortality = number of dead animals/number of test animals × 100%, cure = number of cured animals/number of test animals × 100%. The criteria for cure are: the fish moves, eats and has normal body color, the gill silk is observed by naked eyes without symptoms of congestion, swelling, bleeding and the like, and the mucus secretion of the gill silk is normal.
TABLE 1 test results of the drug of example 1 of the present invention for treating bacterial gill-rot disease of grass carp
According to statistical analysis (shown in table 1), the clinical test result shows that the medicine disclosed by the embodiment 1 of the invention has an obvious effect of treating the gill-rot disease of the grass carp, the cure rate is up to 96.67%, and the average cure rate is 94.44%.
Experimental example 2 comparative observation of prevention and treatment effects of the pharmaceutical composition of the present invention prepared in example 2 and other drugs for preventing and treating bacterial gill-rot disease of grass carp
In late 5 th month of 2013, the experiment was carried out in the clinical laboratory of Dabei agricultural fish farming. The grass carp of clinical trial was purchased from Huairou south China wholesale market in Beijing, with a specification of about 50 g of healthy grass carp, 360 tails, and temporarily cultured in an aquarium in a laboratory for 5 days. The experiment was divided into 4 groups using a cylindrical suspension of Cellophilus (10)4 cfu/mL) on the gill filaments of the experimental fishes of the infection control group and the drug group, and the gill rot symptoms of the fishes are all appeared after 3 days. The group 1 is the drug group of the invention, the traditional Chinese medicine composition prepared in the embodiment 2 of the invention is fed after the gill-rot disease is infected, and the drug of the embodiment 2 of the invention is added according to the weight of 100 mg/kg of fish body for 5 days continuously; the group 2 is medicine 1, and after gill rot disease infection, a control medicine 1 (prepared according to Chinese patent CN 103432261A) is fed according to the weight of 100 mg/kg fish body; the 3 rd group is medicine 2, and after gill rot disease infection, a control medicine 2 (prepared according to Chinese patent CN 1143509A) is fed according to the weight of 100 mg/kg fish; group 4 was a healthy control group, and was not administered for infection. 30 of each group were provided with 3 replicates, as shown in Table 2. The test water is aerated tap water with pH 7.3, ammoniacal nitrogen 0.50 mg/L and nitrous acid nitrogen 0.02 mg/L, and is continuously aerated with water temperature (28 +/-1) DEG C.
The calculation method comprises the following steps: mortality = number of dead animals/number of test animals × 100%, cure = number of cured animals/number of test animals × 100%. The criteria for cure are: the fish moves, eats and has normal body color, and the gill silk is observed by naked eyes without congestion and swelling symptoms, and the mucus secretion of the gill silk is normal.
TABLE 2 test results of the drug of example 2 of the present invention for treating bacterial gill-rot disease of grass carp
According to statistical analysis (shown in table 2), the average cure efficiency of the drug for treating the gill-rot disease of the grass carp in the embodiment 2 of the invention is 95.56%, and the treatment effect is obviously better than that of the control drug 1 group and the control drug 2 group.
Experimental example 3 observation of the prevention and treatment effect of the bacterial gill-rot disease of carp using the pharmaceutical composition of traditional Chinese medicine prepared in the present invention example 3
In 6 months in 2013, carps cultured by a farmer in Anxiang county, Heidel, Hunan province die about 100 fish every day, and the death number is more than one day. The pond is rectangular and has a north-south direction. The water surface area is about 8 mu, the average water depth is 1.5 m, 14 thousands of seedlings are stocked, and the specification is 14-16 seedlings/kg. The sick fish can move freely, slowly, inappetence or not, and blackish body color, especially heavy head, black skin at later stage, and the body surface is almost the same as normal fish at the initial stage of illness, and when severe, the head muscle is uneven, the eye is inward sunken, the eyeball is protruded, and the anus is red and swollen. When the branchial cover is uncovered, the branchial filaments are festered and attached with mucus and sludge, and when the branchial cover is serious, the inner surface of the branchial cover is corroded into a transparent plaque which is similar to a circle, and the transparent plaque is commonly called as a skylight. The pond carp floats at abnormal night (often in advance), dead fish bodies beaten by an aerator impeller on the pond surface float on the water surface, the fish cannot be taken actively or even not taken, and the fish roams in the afternoon or the dead fish in the later half night is serious in the later period of morbidity. When the medicine is used for treatment, the medicine is uniformly mixed with feed according to the proportion of 100 mg/kg of fish weight for 5 days, the death is rapidly controlled, and the phenomenon of dead fish is basically avoided by 4 days.
Test example 4 observation of prevention and treatment effects of acute gill rot disease of grass carp by using the traditional Chinese medicine composition prepared in example 4 of the present invention
In 2013, in the beginning of 9 months, one farmer in Hanshou county, Hunan province suffers from acute gill rot of grass carp. The grass carp is mainly cultured by the culture house, the fish specification is 0.25-0.3 kg/tail, the pond depth of 8 mu is 1.5 m, gill rot disease occurs after water changes, gill deformity occurs, the tail end of gill filaments is white, part of gill parts are black, microscopic examination diseased fishes have no insects and no ascites, and death of the grass carp is increased to about 100 after several days. The medicine is used for treatment, the medicine is uniformly mixed with feed according to the weight ratio of 100 mg/kg of fish body weight for 5 days, the fish can eat freely, the death amount of the fish is not increased on the 1 st day, the death amount of the fish is reduced to more than 60 fish on the 2 nd day, the death amount of the fish is reduced to about 10 fish on the 4 th day, and no dead fish exists on the 5 th day.
Test example 5 observation of the effect of preventing and treating gill-rot disease of carp caused by spider using the Chinese medicinal composition prepared in example 5 of the present invention
In late 9 th month of 2013, the aquaculture pond of Tianjin Diwu Yiyu carp has a specification of about 75 g. About 10 deaths are caused every day when the fish is just started to have diseases, the number of deaths reaches 500 in 5 days, the diseased fish is checked to have gill rot, gill filament bleeding and mucus increasing at different degrees, and a large number of trichodinas are attached to the diseased fish in microscopic examination. The medicine is used for treatment, the medicine is uniformly mixed with feed according to the proportion of 100 mg/kg of fish weight for 5 days, the death rate is rapidly reduced to more than 20 after the medicine is taken for 3 days, no trichodina on the gill part and the body surface is detected, and no dead fish occurs on the 5 th day.
Test example 6 observation of prevention and treatment effects of the traditional Chinese medicine composition prepared in the invention example 6 on gill rot disease of rainbow trout
In the last 10 th month of 2013, in a rainbow trout aquaculture plant in Huairou district, Beijing City, fry weight is about 10-20 g, 48 fish died in day 1, the number of dead fish reaches 400 fish after 7 days, diseased fish show no clustering in water, body is blackened, swimming slowly, and the diseased fish are mostly dispersed on two sides of a water outlet, and food intake of diseased fish is obviously reduced and is less than half of normal food intake. In the disease attack period, a plurality of disinfectants such as strong chlorine, and the like are adopted for in-pond disinfection, and the effect of orally taking antibiotic medicines is not obvious. The diseased fish has complete body surface and the end of each fin declines and is light white. The gill cover is incomplete and falls off, gill threads swell, the end parts are rotten, mucus is arranged around the gill threads, and the color of the gill threads is grey brown. The medicine is used for treatment, the medicine is uniformly mixed with feed according to the weight ratio of 100 mg/kg of fish body weight for 5 days, the dead fish amount is not increased on the 1 st day, the dead fish amount is reduced to more than 10 on the 3 rd day, and no dead fish occurs on the 5 th day.
Experimental example 7 observation of prevention and treatment effects of gill rot disease of grass carp caused by dactylogyrus using the Chinese medicinal composition prepared in example 7 of the present invention
In 10 late ten days of 2013, a pond for raising grass carp mainly is used for mixed culture of the grass carp in Yiyang city of Hunan province, and the weight of the grass carp is about 500 g. Four and fifty fish dead per day and the number of dead fish increased each day. The food intake of sick fishes is obviously reduced, the bodies of the sick fishes are thin, the sick fishes mostly float on the water surface or irregularly sink and float, the floating head phenomenon is frequent, and the fishes do not push up water after the aerator is started. The gill filament of the diseased fish is pale, light red or bright red, mucus is increased, the gill filament is swollen, the gill cover is open, the gill loses blood, dyspnea and dull spirit. The gill silk microscopic examination shows that a large number of finger ring worms are parasitized. The medicine is used for treatment, the medicine is uniformly mixed with feed according to the weight ratio of 100 mg/kg of fish body weight and is continuously fed for 5 days, the dead fish quantity is controlled on the 1 st day, the death number is reduced to 35, more than 10 fish on the 2 nd day and no dead fish on the 3 rd day, the fish in the whole pond is fed for two days in succession, the fish shows active eating, no floating head phenomenon occurs, no parasite of gill silk body is detected by microscopic examination, and gill silk lesion is recovered to be normal.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the structure of the present invention in any way. Any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention still fall within the scope of the technical solution of the present invention.
Claims (3)
1. A Chinese medicinal composition for preventing and treating fish gill rot disease contains active ingredients, wherein the active ingredients are prepared from radix et rhizoma Rhei, herba Houttuyniae, herba Verbenae, radix Sophorae Flavescentis and Galla chinensis; the preparation method of the traditional Chinese medicine composition for preventing and treating fish gill-rot diseases comprises the following steps: weighing the raw materials according to the weight, crushing, adding water which is 5-15 times of the total weight of the five medicines, decocting for 1-3 times, 1-3 hours each time, combining decoctions, filtering, standing the filtrate for 6-12 hours, taking supernate, concentrating, drying and crushing to obtain the traditional Chinese medicine composition;
the raw materials comprise, by weight, 1-50 parts of rheum officinale, 1-30 parts of houttuynia cordata, 1-30 parts of verbena, 1-30 parts of radix sophorae flavescentis and 1-20 parts of gallnut.
2. The pharmaceutical composition of claim 1, wherein the composition is an extract.
3. A method for preparing the traditional Chinese medicine composition for preventing and treating the fish gill-rot disease according to claim 1 or 2, which comprises the following steps:
weighing the raw materials according to the weight parts, crushing, adding water which is 5-15 times of the total weight of the medicines, decocting for 1-3 times, 1-3 hours each time, combining the decoctions, filtering, standing the filtrate for 6-12 hours, taking the supernatant, concentrating, drying and crushing to obtain the traditional Chinese medicine composition.
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| CN105213500A (en) * | 2015-11-11 | 2016-01-06 | 郑州后羿制药有限公司 | A kind of control fresh-water fishes mycotic Chinese herbal medicine fermented extracted liquid and preparation method thereof |
| CN105726900A (en) * | 2016-04-26 | 2016-07-06 | 遵义石林水产养殖有限责任公司 | Composition for preventing and treating fish skin disease and preparation method thereof |
| CN111789898A (en) * | 2020-06-17 | 2020-10-20 | 湖南康易达绿茵科技有限公司 | Aquatic pesticide and production process thereof |
| CN112656863A (en) * | 2020-12-31 | 2021-04-16 | 西藏自治区农牧科学院水产科学研究所 | Traditional Chinese medicine preparation for preventing and treating gill rot disease of Tibet plateau Gymnocypris przewalskii and application |
| CN114848698A (en) * | 2022-06-10 | 2022-08-05 | 保定冀中药业有限公司 | Artemisia apiacea and dichroa febrifuga effervescent granules for treating bacterial gill-rot disease and preparation method thereof |
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| CN101112429A (en) * | 2007-08-02 | 2008-01-30 | 王安林 | Medicine for treating hemorrhage and fin rot of aquatic animals |
| CN102716247A (en) * | 2012-06-28 | 2012-10-10 | 天津生机集团股份有限公司 | Traditional Chinese medicine oral liquid for preventing and treating bacterial gill rotting disease and preparation method thereof |
| CN102764308A (en) * | 2012-08-14 | 2012-11-07 | 天津生机集团股份有限公司 | Drug for preventing bacterial diseases of fish and preparation method thereof |
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| CN101112429A (en) * | 2007-08-02 | 2008-01-30 | 王安林 | Medicine for treating hemorrhage and fin rot of aquatic animals |
| CN102716247A (en) * | 2012-06-28 | 2012-10-10 | 天津生机集团股份有限公司 | Traditional Chinese medicine oral liquid for preventing and treating bacterial gill rotting disease and preparation method thereof |
| CN102764308A (en) * | 2012-08-14 | 2012-11-07 | 天津生机集团股份有限公司 | Drug for preventing bacterial diseases of fish and preparation method thereof |
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